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AIMP2 restricts EV71 replication by recruiting SMURF2 to promote the degradation of 3D polymerase

Virologica Sinica 2024年第4期39卷 632-644页

作者： Junrui Ren Lei Yu Qiuhan Zhang Pengyu Ren Yumeng Cai Xueyun Wang Ke Lan shuwen wu State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan430072China Medical Research Institute Wuhan UniversityWuhan430072China Taikang Center for Life and Medical Sciences Wuhan UniversityWuhan430072China

Hand,foot and mouth disease(HFMD),mainly caused by enterovirus 71(EV71),has frequently occurred in the Asia-Pacific region,posing a significant threat to the health of infants and young ***,research on the infection mechanism and pathogenicity of enteroviruses is increasingly becoming *** 3D polymerase,as the most critical RNA-dependent RNA polymerase(RdRp)for EV71 replication,is widely targeted to inhibit EV71 *** this study,we identified a novel host protein,AIMP2,capable of binding to 3D polymerase and inhibiting EV71 *** investigations revealed that AIMP2 recruits the E3 ligase SMURF2,which mediates the polyubiquitination and degradation of 3D ***,the antiviral effect of AIMP2 extended to the CVA16 and CVB1 *** research has uncovered the dynamic regulatory function of AIMP2 during EV71 infection,revealing a novel antiviral mechanism and providing new insights for the development of antienteroviral therapeutic strategies.

关键词： AIMP2 Enterovirus 71(EV71) 3D polymerase E3 ligase SMURF2 Ubiquitination

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Effect of chiral camphor thiolate ligands on the structure and stability of Au19 nanoclusters

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INORGANIC CHEMISTRY FRONTIERS 2025年第7期12卷 2872-2880页

作者： Zhang, Xiaoya wu, shuwen Li, Qinzhen Chai, Jinsong Gao, Guiqi Huang, Baoyu Yang, Sha Zhu, Manzhou Anhui Univ Inst Phys Sci Hefei 230601 Anhui Peoples R China Anhui Univ Inst Informat Technol Hefei 230601 Anhui Peoples R China Anhui Univ Ctr Atom Engn Adv Mat Dept Chem Key Lab Struct & Funct Regulat Hybrid MatMinist E Hefei 230601 Anhui Peoples R China Anhui Univ Anhui Prov Key Lab Chem Inorgan Organ Hybrid Funct Hefei 230601 Anhui Peoples R China Hunan Inst Engn Coll Mat & Chem Engn Dept Hunan Prov Key Lab Environm Catalysis & Waste Xiangtan 411104 Peoples R China

Based on their well-defined atomic structures, chiral clusters can be utilized to describe the origin of nanomaterial-like chirality, laying the foundation for the application of chiral materials. However, their chiral application is limited owing to chiral phosphines, amines, and carboxylic acids forming protected clusters with weak chiral stability, while thiol-protected clusters are stable;however, there are relatively few types of chiral thiols available yet, and they have a high price. In this study, chiral camphor thiols were designed and prepared from natural products. Based on the novel chiral thiol ligands, a pair of novel R/S-Au19(SR)13DPPM chiral clusters were obtained. Their structural anatomies showed that their chiral origins were diverse and their core distortion was more significant compared with their counterparts. Subsequent stability tests showed that both of these were stable in thermal, alkaline, and reducing environments, thus offering a solid foundation for their application in the corresponding environments. Importantly, the chiral thiol ligands designed in this study could provide new opportunities for the preparation of stable chiral nanoclusters and their applications.

关键词： Ligands

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Transcriptomic study of the role of MeFtsZ2-1 in pigment accumulation in cassava leaves

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BMC GENOMICS 2024年第1期25卷 448页

作者： Zang, Yuwei wu, Kunlin Liu, Liangwang Ran, Fangfang Wang, Changyi wu, shuwen Wang, Dayong Guo, Jianchun Min, Yi Hainan Univ Sch Life & Hlth Dept Biosci Haikou 570228 Hainan Peoples R China Hainan Univ Sch Pharmaceut Sci Lab Biopharmaceut & Mol Pharmacol Haikou 570228 Hainan Peoples R China Hainan Univ Key Lab Trop Biol Resources Minist Educ China Haikou 570228 Hainan Peoples R China Chinese Acad Trop Agr Sci Inst Trop Biotechnol Sanya Inst Sanya 572000 Hainan Peoples R China

MeFtsZ2-1 is a key gene for plant plastid division, but the mechanism by which MeFtsZ2-1 affects pigment accumulation in cassava (Manihot esculenta Crantz) through plastids remains unclear. We found that MeFtsZ2-1 overexpression in cassava (OE) exhibited darker colors of leaves, with increased levels of anthocyanins and carotenoids. Further observation via Transmission Electron Microscopy (TEM) revealed no apparent defects in chloroplast structure but an increase in the number of plastoglobule in OE leaves. RNA-seq results showed 1582 differentially expressed genes (DEGs) in leaves of OE. KEGG pathway analysis indicated that these DEGs were enriched in pathways related to flavonoid, anthocyanin, and carotenoid biosynthesis. This study reveals the role of MeFtsZ2-1 in cassava pigment accumulation from a physiological and transcriptomic perspective, providing a theoretical basis for improving cassava quality.

关键词： MeFtsZ2-1 Cassava Physiology Transcriptome Pigment accumulation

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Geothermal assisted Rankine cycle for performance enhancement of a biomass-driven power plant;Thermoeconomic and environmental impact assessment

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PROCESS SAFETY AND ENVIRONMENTAL PROTECTION 2023年 175卷 341-354页

作者： Fan, Xuezhou Moria, Hazim Reda, Shaker A. Aldawi, Fayez Truong, Nguyen wu, shuwen Zhengzhou Univ Sch Ecol & Environm Zhengzhou 450001 Peoples R China Yanbu Ind Coll Dept Mech Engn Technol Yanbu Al Sinaiyah City 41912 Saudi Arabia HUTECH Univ Inst Engn Ho Chi Minh City Vietnam Univ Kitakyushu Fukuoka 8080135 Japan

In view of the environmental concerns of increasing fossil fuel consumption, the renewable energy sources are promising alternatives, among which the biomass and geothermal energies are the two most common and mature ones. In the present research, a novel Electricity Generation System (EGS) driven by hybrid biomass fuel and geothermal source is developed and its performance is compared with Typical EGS (TEGS) driven by only the biomass fuel. The TEGS consists of a gas turbine cycle and a steam Rankine cycle equipped with an open-feed water heater (OFWH). In the proposed Hybrid Electricity Generation System (HEGS), the geothermal energy is deployed to heat the feedwater before entering the OFWH, as a result the bottoming Rankine cycle yields more electricity. However, a comprehensive investigation from technical and economic standpoints is required to compare its performance with the relevant TEGS. Therefore, through the application of thermodynamic, ther-moeconomic, and environmental methodologies, the considered EGSs performance are modeled and compre-hensively analyzed. Based on the modeling results, the maximum exergetic efficiency for the TEGS and HEGS is achieved, respectively as 40.8% and 43.05% under the gas turbine inlet temperature of 1550 K. Final optimal operating conditions are determined through a multi-criteria optimization respect to exergetic efficiency and cost criteria. Under these working conditions, the LCOE and exergetic efficiency for the TEGS are calculated to be respectively as 71.22 $/MWh and 43.22%. At this point, net electricity, total exergy destruction rate, and CO2 emission rate are estimated to be as 7750 kW, 9446 kW, and 0.7401 kg/kWh, respectively.

关键词： Comparative study Electricity generation system Environmental effects Hybrid renewable energy Thermoeconomic

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Functional redundancy in the toxic pathway of Bt protein Cry1Ab, but not Cry1Fa, against the Asian corn borer

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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2025年第16期122卷 e2503674122页

作者： Wang, Xingliang Yue, Yujin Zhai, Yuqian Wang, Falong Zhuang, Xuna wu, shuwen Yang, Yihua Tabashnik, Bruce E. wu, Yidong Nanjing Agr Univ Coll Plant Protect Dept Entomol State Key Lab Agr & Forestry Biosecur Nanjing Peoples R China Univ Arizona Dept Entomol Tucson AZ 85721 USA

Crops genetically engineered to produce insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) have been used extensively to control some major crop pests, but their benefits decrease when pests evolve resistance. Better understanding of the genetic basis of resistance is needed to effectively monitor, manage, and counter pest resistance to Bt crops. Resistance to Bt proteins in at least 11 species of Lepidoptera, including many important crop pests, is associated with naturally occurring mutations that disrupt one or more of three larval midgut proteins: cadherin and ATP- binding cassette proteins ABCC2 and ABCC3. Here, we determined how CRISPR/Cas9- mediated mutations disrupting cadherin, ABCC2, and ABCC3 singly and in pairs affect resistance to Bt proteins Cry1Ab and Cry1Fa in the Asian corn borer (Ostrinia furnacalis), which is the most damaging pest of corn in Asia and is closely related to the European corn borer (Ostrinia nubilalis), a major pest in Europe and North America. The results from bioassays of six knockout strains and their parent susceptible strain support a model in which Cry1Ab can kill larvae via one path requiring ABCC2 or another path requiring cadherin and ABCC3, whereas Cry1Fa uses only the first path. The model's predictions are generally supported by results from genetic linkage analyses and responses to Cry1Ab and Cry1Fa of Sf9 cells and Xenopus oocytes modified to produce cadherin, ABCC2, and ABCC3 singly or in pairs. The functional redundancy identified here for Cry1Ab could sustain its efficacy against O. furnacalis and may exemplify a widespread natural strategy for delaying resistance.

关键词： Bacillus thuringiensis Ostrinia sustainability resistance management editing

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Integrated "Two-in-One" Strategy for High-Rate Electrocatalytic CO2 Reduction to Formate

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ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2024年第3期64卷 e202415726页

作者： Lei, Peng-Xia Liu, Shao-Qing Wen, Qi-Rui wu, Jia-Yi wu, shuwen Wei, Xiaoxiao Feng, Renfei Fu, Xian-Zhu Luo, Jing-Li Shenzhen Univ Guangdong Res Ctr Interfacial Engn Funct Mat Guangdong Prov Key Lab New Energy Mat Serv Safety Coll Mat Sci & EngnShenzhen Key Lab Energy Electr Shenzhen 518060 Peoples R China Univ Toronto Dept Mat Sci & Engn 184 Coll St Toronto ON M5S 3E4 Canada Canadian Light Source Inc 44 Innovat Blvd Saskatoon SK S7N 0X4 Canada

The electrochemical CO2 reduction reaction (ECR) is a promising pathway to producing valuable chemicals and fuels. Despite extensive studies reported, improving CO2 adsorption for local CO2 enrichment or water dissociation to generate sufficient H* is still not enough to achieve industrial-relevant current densities. Herein, we report a "two-in-one" catalyst, defective Bi nanosheets modified by CrOx (Bi-CrOx), to simultaneously promote CO2 adsorption and water dissociation, thereby enhancing the activity and selectivity of ECR to formate. The Bi-CrOx exhibits an excellent Faradaic efficiency (approximate to 100 %) in a wide potential range from -0.4 to -0.9 V. In addition, it achieves a remarkable formate partial current density of 687 mA cm(-2) at a moderate potential of -0.9 V without iR compensation, the highest value at -0.9 V reported so far. Control experiments and theoretical simulations revealed that the defective Bi facilitates CO2 adsorption/activation while the CrOx accounts for enhancing the protonation process via accelerating H2O dissociation. This work presents a pathway to boosting formate production through tuning CO2 and H2O species at the same time.

关键词： Electrochemical CO2 reduction Formate Defects CO2 adsorption Water dissociation

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Electrodeposited NiFeCoMoW High-Entropy Alloys with Nanoscale Amorphous Structure as Effective Hydrogen Evolution Electrocatalysts

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ACS APPLIED ENERGY MATERIALS 2024年第19期7卷 8412-8422页

作者： Carroll, Zachary Liam Hache, Michel J. R. Wang, Bowen Chen, Lixin wu, shuwen Erb, Uwe Thorpe, Steven Zou, Yu Univ Toronto Dept Mat Sci & Engn Toronto ON M5S 3E4 Canada

The growing demand for hydrogen and the effectiveness of alkaline anion exchange membrane (AEM) electrolyzers has led to an increased interest in finding lower cost alternatives to traditional noble metal electrocatalysts for the hydrogen evolution reaction (HER). High-entropy alloy (HEA) electrocatalysts have received significant attention due to their properties such as high configurational entropy and high lattice distortion which can help promote electrocatalytic reactions. In this work, an aqueously electrodeposited and structurally amorphous NiFeCoMoW HEA was synthesized and investigated for its HER performance. The importance of factors such as surface morphology, chemical composition, and microstructure on the electrochemical activity and stability was also explored. Increased electrochemical activity was observed in the HEA compared to electrodeposited binary alloys, owing to a larger number of active sites with differing electronic structures and adsorption energies. NiFeCoMoW HEAs electrodeposited at a pH of 5 exhibited the smallest Tafel slope of all the tested samples with an average Tafel slope of 83 mV/dec. Meanwhile, the lowest overpotential for 10 mA/cm(GA)(2) of 171 mV was observed in the samples prepared at pH 6, which possessed a higher roughness factor (RF). These results highlight the potential of using HEAs for electrocatalytic applications and demonstrate aqueous electrodeposition as a simple, inexpensive, and scalable synthesis method to produce effective HEA electrocatalysts.

关键词： high-entropy alloy electrocatalysis electrocatalyst hydrogen evolution reaction electrodeposition amorphous

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Lattice Oxygen-Driven Co-Adsorption of Carbon Dioxide and Nitrate on Copper: A Pathway to Efficient Urea Electrosynthesis

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JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2025年第7期147卷 6049-6057页

作者： Wei, Xiaoxiao Liu, Shao-Qing Liu, Hengjie Ding, Yutian Lei, Peng-Xia wu, shuwen Song, Li Fu, Xian-Zhu Luo, Jing-Li Shenzhen Univ Coll Mat Sci & Engn Guangdong Prov Key Lab New Energy Mat Serv Safety Shenzhen Key Lab Energy Electrocatalyt Mat Shenzhen 518060 Peoples R China Univ Sci & Technol China Natl Synchrotron Radiat Lab Hefei 230052 Anhui Peoples R China Univ Toronto Dept Mat Sci & Engn Toronto ON M5S 3E4 Canada

The electrochemical coupling of CO2 and NO3 - on copper-based catalysts presents a sustainable strategy for urea production while simultaneously addressing wastewater denitrification. However, the inefficient random adsorption of CO2 and NO3 - on the copper surface limits the interaction of the key carbon and nitrogen intermediates, thereby impeding efficient C-N coupling. In this study, we demonstrate that the residual lattice oxygen in oxide-derived copper nanosheets (OL-Cu) can effectively tune the electron distribution, thus activating neighboring copper atoms and generating electron-deficient copper (Cu delta+) sites. These Cu delta+ sites enhance CO2 adsorption and stabilize *CO intermediates, which enables the directional NO3 - adsorption at adjacent Cu delta+ sites. This mechanism shortens the C-N coupling pathway and achieves a urea yield of up to 298.67 mmol h-1 g-1 at -0.7 V versus RHE, with an average Faradaic efficiency of 31.71% at a high current density of similar to 95 mA cm-2. In situ spectroscopic measurements confirmed the formation of Cu delta+ sites and tracked the evolution of the key intermediates (i.e., *CO, *NO, *OCNO, and *NOCONO) during urea synthesis. Density functional theory calculations revealed that Cu delta+ sites promote adjacent coadsorption of *CO and *NO3, as well as *OCNO and *NO3, significantly improving C-N coupling kinetics. This study underscores the critical role of lattice oxygen in facilitating adjacent coadsorption and improving C-N coupling selectivity.

关键词： Urea

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A Low-Voltage and LDO-Less Wireless Pressure Sensor Readout System with Current Mode Pseudo PLL and Charge Pump Powered UWB Data Transmission for Implantable Health Monitoring

A Low-Voltage and LDO-Less Wireless Pressure Sensor Readout ...

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Midwest Symposium on Circuits and Systems (MWSCAS)

作者： Fanyang Li shuwen wu Kaiji Liu College of Physics and Information Engineering Fuzhou University China School of Integrated Circuits Tsinghua University China

ISBN: (数字)9798350387179

ISBN: (纸本)9798350387186

This paper presents an implantable pressure sensor readout system (IPRO) which can work at low voltage and does not need internal low dropout regulator (LDO). This paper has made a series of optimizations for power consumption. The power supply part of the IPRO uses 434 MHz energy harvesting (EH), and the corresponding ultra-low power undervoltage lockout (UVLO) circuit is used. The analog front-end (AFE) and signal processing parts use current mode and differential pseudo phase locked loop (DPPLL), which can work stably under low voltage and maintain a certain degree of accuracy and power rejection ability. The wireless data transmission part adopts the impulse ultra-wide band (IR-UWB) scheme. Its ultra-low power consumption allows it to be powered by a charge pump. In order to further optimize power consumption, a simplest encoding circuit suitable for the front-end output form was proposed, and a power oscillator (PO) with accelerated starup was used to optimize area and power consumption. The system is implemented in 180 nm Complementary Metal Oxide Semiconductor (CMOS) process, with a chip area of $1.1 ^*1.0$ mm”, The measurement results indicate that the typical working voltage of the IPRO is 0.8 V, the typical power consumption is 11.8 $\mu \mathrm{W}$ , and the accuracy is 1.11 mmHg. Compared with existing designs, there is a significant optimization in supply voltage and power consumption.

关键词： Wireless communication Pressure sensors Low voltage Wireless sensor networks Power demand Accuracy Low power electronics

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Efficient Synthesis of Glycodiversified Nucleoside Analogues by a Thermophilic Promiscuous Glycosyltransferase

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ACS CATALYSIS 2025年第2期15卷 1217-1229页

作者： Wang, Zhuqing Li, Jiahui Wang, Xueyun Jin, Boyu Zhou, Liwei Zhao, Zili Gu, Meijia Song, Xuemin Wang, Jiahong Deng, Zixin wu, shuwen Zhang, Zhengyu Chen, Wenqing Wuhan Univ Zhongnan Hosp TaiKang Ctr Life & Med Sci Sch Pharmaceut SciDept Anesthesiol Wuhan 430071 Peoples R China Wuhan Univ Key Lab Combinatorial Biosynth & Drug Discovery Minist Educ Wuhan 430071 Peoples R China Wuhan Univ Coll Life Sci State Key Lab Virol Wuhan 430072 Peoples R China Nanjing Forestry Univ Int Innovat Ctr Forest Chem & Mat Nanjing 210037 Peoples R China

3 '-O-beta-Glucosyl purine-related nucleosides are actinobacterial natural products with intricate structures, in which the glucosyl attachment to a nucleoside scaffold is governed by a glycosyltransferase. However, the molecular logic and engineered application of the glycosyltransferase have nearly remained unexplored. Here, we report the discovery, characterization, and exploitation of the thermophilic glycosyltransferase ScaGT. We uncover that ScaGT and its homologue AvpGT indicate prominent promiscuity against both sugar donors and a variety of nucleosides. Remarkably, we have solved the ternary complex structure of AvpGT, unveiling that it employs an unpreceded "twin-tyrosine gate" mechanism for substrate recognition and promiscuity, and we have also realized directed biosynthesis of diversified purine nucleoside analogues with unexpectedly enhanced titer via introduction of the external scaGT or avpGT. Moreover, we reveal that 3 '-O-beta-glucosyl ribavirin exhibits significantly enhanced antiviral activities, thereof showing its promising application potentials. Finally, we have further achieved the gram-scale production of 3 '-O-beta-glucosyl ribavirin by both biocatalytic and fermentation-feeding strategies. These findings expand the biochemical repertoire regarding glycosyltransferase reactions and provide the basis for rapid mining and rational engineering of more related glycosyltransferases toward synthetic biology applications.

关键词： glycosyltransferase promiscuity purine nucleosideanalogues 3 '-O-beta-glucosylribavirin biocatalysis

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