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IEEE Design and Diagnostics of Electronic Circuits and Systems (DDECS)

作者： Jie Pan Fanyang Li Yidong Yuan Tianting Zhao Hongwei Shen Liguo Wen Yi Hu Jiazhen Jin shuwen wu Beijing Smart-chip Microelectronics Technology Co. Ltd. Beijing China College of Physics and Information Engineering Fuzhou University Fuzhou China

This paper proposes a low-noise high-voltage rail-to-rail operational amplifier based on BCD process. For the pro-tection of low-voltage devices in input stage, this paper proposes a common-mode voltage following circuit which not only realizes the stable performance of low-voltage input devices but also improves the gain under full supply voltage. In order to improve the response speed of large signals, this paper proposes a dynamic slew-rate enhancement technology to achieve the characteristics of low quiescent current and large slew-rate. Besides, this paper optimized the low-frequency noise of the proposed architecture. The circuit uses 180nmBCD process, and the simulation results show that after applying the above technology, the supply voltage range is 3.5-36 V, the quiescent current is about 0.99 mA, the equivalent input noise is 0.82 uVp-p, the slew-rate is 15.2 V/us, the setting time is 0.66 us and the open loop gain is 135 dB in full supply voltage range.

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A case of chronic wounds caused by Sporothrix schenckii infection was rapidly detected by metagenomic next generation sequencing

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HELIYON 2024年第2期10卷 e24420页

作者： Jin, Wenxiang Liu, Yong Ning, Qiuyue wu, shuwen Su, Sibiao Zheng, Dongyan Ma, Shasha Zou, Jun Yang, Min Hu, Diefei Ding, Huarong Dept Infect Dis KingMed Diagnost Guangzhou 510005 Guangdong Peoples R China Guangxi Med Univ Affiliated Hosp 1 Dept Infect Dis Nanning 530021 Guangxi Peoples R China Guangxi Med Univ Affiliated Hosp 1 Dept Gastroenterol Nanning 530021 Guangxi Peoples R China Guangxi Med Univ Affiliated Hosp 1 Dept Dermatol & Venereol Nanning 530021 Guangxi Peoples R China Fourth Peoples Hosp Nanning Dept Infect Dis Nanning 530023 Guangxi Peoples R China Guangxi Med Univ Affiliated Hosp 1 Dept Burns & Plast Surg Nanning 530021 Guangxi Peoples R China

The dimorphic fungus Sporothrix schenckii is widely distributed in soil, vegetation, and decaying organic matter, and can cause sporotrichosis when the patient's skin trauma was exposed to contaminated material with Sporothrix spp. The cases of Sporothrix schenckii infection in chronic wounds are rarely reported. Here we reported a 53 -year -old male construction worker who was admitted to our hospital on July 9, 2022, without underlying disease presented with a painless subcutaneous hard nodule on his right calf, which later ulcerated and oozed, with an enlarged wound and no fever during the course of the disease. His procalcitonin, C -reactive protein, erythrocyte sedimentation rate increased, and necrotic histopathology suggested chronic granulomatous inflammation. Then his necrotic tissue and pus were sent for metagenomic next generation sequencing(mNGS), the result reported Sporothrix schenckii after 43 hours, which was consistent with the result of culture after 18 days. mNGS might be more useful and valuable in diseases such as sporotrichosis where it is difficult to see the yeast cells in the tissues.

关键词： Sporotrichosis mNGS Culture Dimorphic fungus

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Identification of the 4cl Family in Cassava and Expression Pattern Analysis of the Me4cl32 Gene

SSRN

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SSRN 2023年

作者： Ran, Fangfang Wang, Changyi Zang, Yuwei Liu, Liangwang wu, shuwen Wang, Congcong Wang, Dayong Min, Yi Department of Biosciences School of Life and Health Hainan University Hainan Haikou570228 China Laboratory of Biopharmaceuticals and Molecular Pharmacology School of Pharmaceutical Sciences Hainan University Hainan Haikou570228 China

The 4-coumarate coenzyme A ligase (4CL) plays a critical role in the phenylpropane metabolic pathway and is a key enzyme in plant growth metabolism and stress responses. In this study, 50 Me4CLs were identified within the cassava genome utilizing bioinformatics methods, and a comprehensive analysis of the cassava 4CL gene family was conducted. The results showed that the 50 4CL proteins were divided into four subfamilies, and members within the same subfamily share similar or identical gene structures. Co-linearity analysis revealed that cassava and rubber trees have the highest number of homologous genes, indicating a close homologous relationship between them. Analysis of 15 cis-acting elements in the promoter region of Me4CL32 reveals the presence of hormone-responsive elements such as gibberellin, auxin, abscisic acid, and as well as elements related to meristematic tissue regulation. The results of quantitative real-time PCR (qRT-PCR) showed alterations in Me4CL32 gene expression in response to abiotic stressors (drought, salt, cold, heat) as well as GA3 and ABA hormonal stimuli, implying the capacity of Me4CL32 to react to both environmental pressures and hormone stimuli. The RNA-seq transcriptome and single-cell transcriptome were opted to analyze the expression pattern of Me4CLs. Additionally, the subcellular localization of the Me4CL32 demonstrated the confinement of the Me4CL32 protein within cassava leaf chloroplasts. Delving into the functionality of this gene family aids in comprehending the growth, development, and stress resistance mechanisms within plants. Furthermore, it furnishes a theoretical underpinning for future investigations concerning resilient cassava germplasm and the enhancement of cassava's environmental tolerance. © 2023, The Authors. All rights reserved.

关键词： Proteins

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Single amino acid variations drive functional divergence of cytochrome P450s in Helicoverpa species

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INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 2022年第0期146卷 000-000页

作者： Shi, Yu Sun, Shuo Zhang, Yujun He, Yingshi Du, Minghong OReilly, Andrias O. wu, shuwen Yang, Yihua wu, Yidong Nanjing Agr Univ Coll Plant Protect Nanjing 210095 Peoples R China Liverpool John Moores Univ Sch Biol & Environm Sci Liverpool England

Divergence of gene function is a hallmark of evolution, but assessing such divergence in one species or between species requires information on functional alterations of the alleles and homologs. Here, we explore the functional divergence of two paralogs, CYP6AE19 and CYP6AE20, from Helicoverpa armigera, and two close orthologs, CYP6B8 and CYP6B7, from two related species (Helicoverpa zea and H. armigera);although there is high sequence identity within each pair of enzymes, the latter P450 of each pair has lost metabolic competence towards the plant allelochemical xanthotoxin. Multiple chimeric and single/double site mutants were created by exchanging the diverse substrate recognition sites (SRSs) and amino acids within each pair of P450s. Heterologous expression in Sf9 cells and in vitro metabolism studies showed that the exchange of SRS4 swapped the activity of CYP6AE19 and CYP6AE20, and subsequent site-directed mutagenesis demonstrated that the CYP6AE20 V318M substitution causes a gain-of-function towards xanthotoxin. Meanwhile, a single amino acid substitution (L489P) in SRS6 was found to swap activity between the CYP6B orthologs. Sequence alignments of CYP6AE paralogs and all reported insect xanthotoxin-metabolizing P450s suggest M318 and P489 are essential for the catalytic activities of CYP6AE paralogs and CYP6B orthologs, respectively, but P450s in different subfamilies may have different mechanisms towards the same substrate. Our findings demonstrate that a single amino acid substitution can suffice to alter substrate metabolism and this functional divergence resulting from natural mutations will help to further our understanding of the process of natural selection of P450 genes and their role in insect-host plant interactions.

关键词： Cytochrome P450 Functional divergence Detoxification Xanthotoxin Molecular modelling

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HIMO: A New Benchmark for Full-Body Human Interacting with Multiple Objects

arXiv

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arXiv 2024年

作者： Lv, Xintao Xu, Liang Yan, Yichao Jin, Xin Xu, Congsheng wu, shuwen Liu, Yifan Li, Lincheng Bi, Mengxiao Zeng, Wenjun Yang, Xiaokang MoE Key Lab of Artificial Intelligence AI Institute Shanghai Jiao Tong University Shanghai China Ningbo Institute of Digital Twin Eastern Institute of Technology Ningbo China NetEase Fuxi AI Lab China

Generating human-object interactions (HOIs) is critical with the tremendous advances of digital avatars. Existing datasets are typically limited to humans interacting with a single object while neglecting the ubiquitous manipulation of multiple objects. Thus, we propose HIMO, a large-scale MoCap dataset of full-body human interacting with multiple objects, containing 3.3K 4D HOI sequences and 4.08M 3D HOI frames. We also annotate HIMO with detailed textual descriptions and temporal segments, benchmarking two novel tasks of HOI synthesis conditioned on either the whole text prompt or the segmented text prompts as fine-grained timeline control. To address these novel tasks, we propose a dual-branch conditional diffusion model with a mutual interaction module for HOI synthesis. Besides, an auto-regressive generation pipeline is also designed to obtain smooth transitions between HOI segments. Experimental results demonstrate the generalization ability to unseen object geometries and temporal compositions. Our data, codes, and models will be publicly available for research purposes. Copyright © 2024, The Authors. All rights reserved.

关键词： Benchmarking

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Plasma proteomic analysis reveals altered protein abundances in HIV-infected patients with or without non-Hodgkin lymphoma

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JOURNAL OF MEDICAL VIROLOGY 2022年第8期94卷 3876-3889页

作者： Zhuang, Ke Zhang, Yongxi Mo, Pingzheng Deng, Liping Jiang, Yong Yu, Lei Mei, Fanghua Huang, Shaoxin Chen, Xi Yan, Yajun Tang, Hongbin Li, Xiangdong Xiong, Yong wu, shuwen Ke, Hengning Gui, Xien Lan, Ke Wuhan Univ ABSL 3 Lab State Key Lab Virol Ctr Anim Expt Wuhan Hubei Peoples R China Wuhan Univ Dept Infect Dis Zhongnan Hosp Wuhan Hubei Peoples R China Wuhan Univ Coll Life Sci Modern Virol Res Ctr State Key Lab Virol Wuhan Hubei Peoples R China Hubei Prov Ctr Dis Control & Prevent Wuhan Hubei Peoples R China SpecAlly Life Technol Co Ltd Wuhan Hubei Peoples R China

The identification of circulating proteins associated with acquired immunodeficiency syndrome-related non-Hodgkin lymphoma (AIDS-NHL) may help in the development of promising biomarkers for screening, diagnosis, treatment, and prognosis. Here, we used quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify differentially expressed proteins (DEPs) in plasma collected from patients with AIDS-NHL and human immunodeficiency virus (HIV)-infected patients without NHL (HIV+). Proteins with a log2 (fold change) in abundance >0.26 and p < 0.05 were considered differentially abundant. In total, 84 DEPs were identified, among which 20 were further validated as potential biomarkers, with immunoglobulin and complement components being the most common proteins. Some of the proteins were further verified in a retrospective analysis of the medical records of patients in a larger cohort. These markedly altered proteins were found to mediate pathophysiological pathways that likely contribute to AIDS-NHL pathogenesis, such as the humoral immune response, complement activation, and complement and coagulation cascades. Our findings provide a new molecular understanding of AIDS-NHL pathogenesis and provide new evidence supporting the identification of these proteins as possible biomarkers in AIDS-NHL.

关键词： acquired immunodeficiency syndrome-related non-Hodgkin lymphoma (AIDS-NHL) bioinformatics human immunodeficiency virus (HIV) proteomics

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Hexafluoroisopropanol-salt aqueous two-phase system for extraction and purification of chlorogenic acid from ramie leaves

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JOURNAL OF CHROMATOGRAPHY A 2019年 1597卷 196-201页

作者： Huang, Anqi Deng, Wenwen wu, Dandan wu, shuwen Xiao, Yuxiu Wuhan Univ Minist Educ Key Lab Combinatorial Biosynth & Drug Discovery Wuhan 430071 Peoples R China Wuhan Univ Sch Pharmaceut Sci Wuhan 430071 Peoples R China Wuhan Univ Coll Life Sci State Key Lab Virol Wuhan 430072 Peoples R China

Hexafluoroisopropanol (HFIP) was used as a phase-separation solvent to develop novel alcohol-salt aqueous two-phase systems (ATPSs) with various salts. Phase diagram and effective excluded volume (EEV) study proved that HFIP has much better phase-separation ability compared to traditional small molecule alcohols (ethanol, isopropanol and n-propanol). Then, the HFIP-NaCl ATPS was applied for the extraction and purification of chlorogenic acid (CGA) from ramie leaves. Under the optimum conditions (2 M NaCl solution with pH 3.0, the volume ratio of NaCl solution to HFIP at 6, vortex time 5 s and centrifugation time 7 min), the extraction efficiency of CGA in the salt-rich phase was 99.3%, meanwhile the HFIP-rich phase could extract a large amount of impurities. Furthermore, the CGA product with the purity of 91.0% was obtained from the salt-rich phase by semi-preparative liquid chromatography and salt removal, and its chemical structure was identified. Compared with other ATPSs, the HFIP-NaCl ATPS consumed much less organic solvent and salt, but acquired much higher extraction efficiency and obvious impurity-removal effect. Therefore, the HFIP-based alcohol-salt ATPSs are promising in the extraction and purification of CGA and other polar compounds as well. (C) 2019 Elsevier B.V. All rights reserved.

关键词： Hexafluoroisopropanol Aqueous two-phase system Chlorogenic acid Ramie leaves

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基于深度学习的3D点云目标检测研究综述

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遥测遥控 2024年第5期45卷 1-18页

作者：武淑文李燕烯张少琛杨金福北京工业大学北京100124

近年来,3D目标检测作为自动驾驶、移动机器人、虚拟现实等应用产业的重要基础任务,受到了各领域研究人员的广泛关注。其旨在三维空间中对感兴趣目标进行定位与分类,给出相应的3D包围盒,包括目标的位置、大小和方向,为后续对三维场景的... 详细信息

近年来,3D目标检测作为自动驾驶、移动机器人、虚拟现实等应用产业的重要基础任务,受到了各领域研究人员的广泛关注。其旨在三维空间中对感兴趣目标进行定位与分类,给出相应的3D包围盒,包括目标的位置、大小和方向,为后续对三维场景的理解与感知、对车辆的规划与决策提供基础信息。激光雷达传感器捕获的点云因其具有准确的三维信息与深度信息,成为3D目标检测最为常用的输入数据。本文对基于深度学习的3D激光雷达点云目标检测进行综述,总结了点云的数据特点与处理方法,介绍了相应的几类检测方法以及点云和图像融合的多模态检测方法,对不同方法的性能进行对比分析,最后讨论3D点云目标检测未来面临的挑战和发展趋势。

关键词： 3D目标检测点云激光雷达自动驾驶深度学习

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Costimulatory Molecule CD226 Regulates Atopic Dermatitis in a Mouse Model

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JOURNAL OF INVESTIGATIVE DERMATOLOGY 2024年第8期144卷 1743-1753.e4页

作者： Qiao, Wei Duan, Chujun Ma, Jingchang Hu, Wei Xie, Yang Yang, Lu Wang, Tingting wu, shuwen Li, Xuemei Wang, Yuling Cheng, Kun Zhang, Yun Zhang, Yuan Zhuang, Ran Northwestern Polytech Univ Inst Med Res Xian Peoples R China Fourth Mil Med Univ Dept Immunol Xian Peoples R China Fourth Mil Med Univ Otolaryngol Dept Tangdu Hosp Xian Peoples R China Fourth Mil Med Univ Xijing Hosp Dept Dermatol Xian Peoples R China

This study investigated the role of CD226 in a 2,4-dinitrochlorobenzene (DNCB)-induced mouse model of atopic dermatitis. The results showed that the lack of CD226 (global and CD4+ T-cell specific) significantly increased ear thickness, reddening, swelling, and scaling of the skin as well as inflammatory cell and mast cell infiltration. RT-qPCR results demonstrated that the mRNA expressions of atopic dermatitis-related inflammatory cytokines and chemokines were markedly increased in the draining lymph nodes and lesioned ear skin tissues of global and CD4+ T-cell-specific CD226-deficient mice compared with that in control mice. In vitro assessment revealed that CD226 directly modulates TGFb-mediated regulatory T (Treg) cell differentiation and proliferation. Notably, Treg cell-specific deletion of CD226 (Cd226fl/flFoxp3cre mice) resulted in more severe dermatitis and epidermal thickening than those observed in littermate mice upon DNCB treatment. Subsequent analysis showed that the infiltration of Treg cells in ear lesions and the number of Tregs in the spleen were significantly reduced in Cd226fl/flFoxp3cre mice after DNCB treatment. In addition, the lack of CD226 induced apoptosis of Treg cells through the activation of caspase 3. Therefore, these results suggest that CD226 has potential efficacy in atopic dermatitis, correlating with Treg cell inhibition.

关键词： atopic dermatitis CD226 CD4+T cell regulatory T cell

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Ablation of CD226 on CD⁴⁺ T cells modulates asthma progress associated with altered IL-10 response and gut microbiota

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INTERNATIONAL IMMUNOPHARMACOLOGY 2023年第1期118卷

作者： Xie, Yang Zhang, Yuan Wang, Tingting Liu, Yitian Ma, Jingchang wu, shuwen Duan, Chujun Qiao, Wei Cheng, Kun Lu, Lianjun Zhuang, Ran Bian, Ka Fourth Mil Med Univ Tangdu Hosp Dept Otorhinolaryngol 1 Xinsi Rd Xian 710038 Shaanxi Peoples R China Northwestern Polytech Univ Inst Med Res 127 West Youyi Rd Xian 710032 Shaanxi Peoples R China Fourth Mil Med Univ Dept Immunol 169 West Changle Rd Xian 710032 Shaanxi Peoples R China Fourth Mil Med Univ Tangdu Hosp Dept Osteol 1 Xinsi Rd Xian 710032 Shaanxi Peoples R China

To investigate the role of the costimulatory molecule CD226 in asthma pathogenesis, we produced a CD4+ T-cell -specific CD226 knockout mice model (Cd226(Delta CD4)) and induced airway allergic inflammation by administering ovalbumin (OVA). Our results revealed alleviated lung inflammation, decreased levels of OVA-specific IgE, and increased levels of IL-10 in the serum of Cd226(Delta CD4) mice (P < 0.05). Moreover, IL-10 levels in CD4+ T cells were significantly elevated in the mediastinal lymph node, spleen, and Peyer's patches in the Cd226(Delta CD4) mice compared with those in controls (P < 0.05 to P < 0.01). Notably, there was a significantly higher IL-10 mRNA levels in the large intestine of the mice (P < 0.05). The protective effect of CD226 deficiency is also associated with the accumulation of gut TCR gamma delta+ intraepithelial lymphocytes and reversion of the gut microbiome dysbiosis. The Bacteroidetes-to-Firmicutes ratio and the abundance of Akkermansia increased in the absence of CD226 after OVA treatment. Our data reveal the synchronous changes in the lung and intestine in OVA-treated CD226-knockout mice, supporting the gut-lung axis concept and providing evidence for novel therapeutic approaches for asthma.

关键词： Asthma T cell CD226 IL-10 Intestinal intraepithelial lymphocytes Gut microbiota

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