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Up-regulated microRNAs in blastocoel fluid of human implanted embryos could control circuits of pluripotency and be related to embryo competence

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JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 2025年 1-15页

作者： Battaglia, Rosalia Caponnetto, Angela Ferrara, Carmen Fazzio, Anna Barbagallo, Cristina Stella, Michele Barbagallo, Davide Ragusa, Marco Vento, Maria Elena Borzi, Placido Scollo, Paolo Carli, Luca Feichtinger, Michael Kasapi, Evangelia Tsakos, Elias Palini, Simone sierka, wojciech Pecorino, Basilio Campitiello, Maria Rosaria Ronsini, Carlo Purrello, Michele Valerio, Domenico Longobardi, Salvatore D'Hooghe, Thomas Di Pietro, Cinzia Univ Catania Dept Biomed & Biotechnol Sci Sect Biol & Genet G Sichel I-95123 Catania Italy Univ Enna Kore Dept Med & Surg I-94100 Enna Italy Cannizzaro Hosp IVF Unit Catania Italy Wunschbaby Inst Feichtinger Vienna Austria EmbryoClin IVF Thessaloniki Greece Cervesi Hosp IVF Unit Cattolica Italy Gyncentrum Sp Zelazna 1 PL-40851 Katowice Poland ASL Salerno Dept Obstet & Gynecol & Physiopathol Human Reprod Salerno Italy Univ Campania Luigi Vanvitelli Dept Woman Child & Gen & Specialized Surg Naples Italy Inst Genet Res IRG I-80143 Naples Italy Merck Serono SpA Global Clin Dev Rome Italy Merck KGaA Darmstadt Germany KU Leuven Univ Leuven Dept Dev & Regenerat Biomed Sci Grp Leuven Belgium

Purpose The paper aims to investigate the biological role of microRNAs secreted by preimplantation embryo into the blastocoel fluid and to detect a distinctive molecular signature for identifying embryos with the highest implantation potential. Methods We carried on a multicenter retrospective study involving five European IVF centers. We collected 112 blastocoel fluid samples from embryos on day 5 post-fertilization, cultured individually, along with data on blastocyst grade and embryo transfer outcomes. Using a custom TLDA Array, we compared the expression levels of 89 miRNAs between 33 fluids from high-quality implanted embryos and 30 fluids from high-quality not-implanted embryos. Expression differences were assessed using SAM and t-test. Additionally, correlation and function enrichment analysis and network construction were conducted to identify the biological roles of deregulated microRNAs. Results We identified six up-regulated microRNAs in the blastocoel fluid from implanted embryos, significantly and positively correlated across all samples (r >= 0.7;P <= 0.05). They could take part in pluripotency circuits, regulating and being regulated by transcription factors associated with stemness, cell growth, and embryo development. The ROC curve analysis confirmed the potential of these miRNAs as implantation classifiers. Conclusion The six miRNAs up-regulated in blastocoel fluid from implanted embryos may represent a functional molecular signature for evaluating blastocyst quality and identifying the most competent embryos. Their evaluation associated with non-invasive preimplantation genetic testing, integrating epigenetic and genomic analyses, could enhance implantation grade and allow for identification of the euploid embryo not able to implant.

关键词： Blastocoel fluid Embryo development Embryo implantation Human blastocyst MicroRNAs

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First records of Chirothrips ambulans (Thysanoptera, Thripidae) in Slovakia

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BIOLOGIA 2006年第5期61卷 641-642页

作者： sierka, wojciech Silesian Univ Dept Zool Fac Biol & Environm Protect PL-40007 Katowice Poland

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A comparative analysis of non-invasive preimplantation genetic testing for aneuploidies using next-generation sequencing on spent culture media versus trophectoderm

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EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY 2024年 293卷 220-220页

作者： Bednarska-Czerwinska, Anna Strychalska, Anna sierka, wojciech Mercik, Dariusz Morawiec, Emilia Pudelko, Adam Smolen-Dzirba, Joanna Kokot, Anna Jodlowiec-Lubanska, Natalia Simka-Lampa, Klaudia Wroblewska, Urszula Mermer, Patrycja Gyncentrum Genet Iaboratory Sosnowiec Poland Univ Technol Katowice Fac Med Zabrze Poland

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Effects of plant functional group and reclamation treatments on microbial networks and nutrient limitation in initial soil developed on spoil heaps after hard coal mining

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APPLIED SOIL ECOLOGY 2025年 208卷

作者： Bierza, wojciech Trzebny, Artur Kompala-Baba, Agnieszka Wos, Bartlomiej Pietrzykowski, Marcin Jozefowska, Agnieszka Pajak, Marek Otremba, Krzysztof sierka, Edyta Univ Silesia Katowice Inst Biol Biotechnol & Environm Protect Ul Jagiellonska 28 PL-40032 Katowice Poland Adam Mickiewicz Univ Fac Biol Mol Biol Tech Lab ul Uniwersytetu Poznanskiego 6 PL-61614 Poznan Poland Agr Univ Krakow Fac Forestry Dept Ecol Engn & Forest Hydrol Al 29 Listopada 46 PL-31425 Krakow Poland Agr Univ Krakow Fac Agr & Econ Dept Soil Sci & Agrophys al Mickiewicza 21 PL-30120 Krakow Poland Poznan Univ Life Sci Fac Environm Engn & Mech Engn Dept Soil Sci Land Reclamat & Geodesy Ul Piatkowska 94 PL-60649 Poznan Poland

Plant functional groups (PFGs), together with the type of reclamation treatment applied, can influence the changes in soil nutrient availability. The stoichiometric imbalance between resources can alter the activity and structure of microbial communities which play a crucial role in regulating carbon and nutrient cycling in initial soil ecosystems. However, the response of microbial networks within the soil food web in spoil heaps to stoichiometric imbalances has not yet been investigated. In this study, we examine how soil microbial networks webs respond to elemental limitation in unreclaimed sites on carboniferous bare rock and reclaimed with topsoiling in the presence of two PFGs, i.e. grasses and forbs, which are commonly used in the spoil heap reclamation processes. To understand these relationships, soil physicochemical properties, enzyme stoichiometry, abundances and community compositions of soil bacteria and fungi were investigated. We found that the availability of P most limited the formation of soil microbial networks. Both types of PFGs stimulated the formation of complex soil microbial networks but didn't affect the nutrient limitation of the soil microbial community differently. Soil microbiome in bare rock was P-limited and characterized by disturbed structure and lower diversity. Topsoiling increased biodiversity and improved the structure of soil microbial communities. The application of topsoiling showed no positive effect on the stability and complexity of the soil microbial networks. Our findings indicate that P-limitation needs to be considered in understanding and predicting biogeochemical cycles on unreclaimed heaps where spontaneous succession occurs. Topsoiling appears to be a good way to reclaim heaps with an adequate nitrogen supply to the soil.

关键词： Extracellular enzymes stoichiometry Ecological network Forbs Grasses Novel ecosystems Topsoiling

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Photosynthetic response of Solidago gigantea Aition and Calamagrostis epigejos L. (Roth) to complex environmental stress on heavy metal contaminated sites

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SCIENTIFIC REPORTS 2024年第1期14卷 1-20页

作者： Baba, wojciech Kompala-Baba, Agnieszka sierka, Edyta Bierza, wojciech Natl Res Inst Inst Technol & Life Sci Al Hrabska 3 PL-05090 Raszyn Poland Univ Silesia Katowice Inst Biol Biotechnol & Environm Protect Fac Nat Sci PL-40032 Katowice Poland

Studies of in situ plant response and adaptation to complex environmental stresses, are crucial for understanding the mechanisms of formation and functioning of ecosystems of anthropogenically transformed habitats. We study short- and long-term responses of photosynthetic apparatus (PSA) and anti-oxidant capacity to complex abiotic stresses of common plants Calamagrostis epigejos and Solidago gigantea in semi-natural (C) and heavy metal contaminated habitats (LZ). We found significant differences in leaf pigment content between both plant species growing on LZ plots and their respective C populations. The average values of leaf chlorophyll indexes were 27% lower in the LZ populations of both species and significantly lower in Sg plants in comparison to Ce ones. The average values of the anthocyanin index in CeLZ and SgLZ populations were significantly higher (by 18%) than in their respective controls. In both Ce and Sg plants occurring on LZ plots, the average leaf flavonol indexes were higher than on their controls by 31% and 15% and this index was significantly higher in SgLZ population than CeLZ and CeC plants (by 34% and 54%, respectively). Both Ce and Sg populations growing on LZ plots showed significantly lower photosynthetic rate (A), transpiration rate (E) and stomatal conductance (gs) in comparison to controls. On the other hand, a significantly higher photosynthetic rate was detected in SgLZ than in CeLZ populations. The catalase activities were significantly higher than recorded in Sg than in Ce tissues, irrespective of the plot type. They were also higher in LZ populations than those in controls for both species. Moreover, the H2O2 content in Sg tissues was significantly higher than those in Ce. Hydrogen peroxide content in CeLZ and SgLZ were respectively 39% (non-significant) and 57% higher, compared to their controls. The reverse pattern was found in the case of MDA, whose concentration was significantly higher in the leaves of Ce population compared t

关键词： Lead Zinc Plants Ecophysiology Expansive herbs Chlorophyll fluorescence Post-industrial wastelands

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Eco-physiological responses of Calamagrostis epigejos L (Roth) and Solidago gigantea Aition to complex environmental stresses in coal-mine spoil heaps

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LAND DEGRADATION & DEVELOPMENT 2021年第18期32卷 5427-5442页

作者： Kompala-Baba, Agnieszka sierka, Edyta Bierza, wojciech Baba, wojciech Blonska, Agnieszka Wozniak, Gabriela Univ Silesia Katowice Inst Biol Biotechnol & Environm Protect Fac Nat Sci Jagiellonska 28 PL-40032 Katowice Poland Inst Ecol Ind Areas Katowice Poland

We present a study of short and long-term responses of photosynthetic apparatus and anti-oxidant capacity to complex abiotic stresses of Calamagrostis epigejos and Solidago gigantea commonly occurring in seminatural habitats and novel ecosystems of coal-mine spoil heaps. Drought/salinity and elevated temperature were dominant abiotic stressors triggering both species- and habitat-specific responses, confirmed by ChlF induction curves analysis. Although in both species a decrease in net photosynthetic rate (A) and transpiration rate (E) in spoil heap were observed, Ce showed higher (A) on both habitats in comparison to Sg. Moreover, we found higher H2O2 concentration in Sg leaves as compared to Ce leaves, large differences in catalase (CAT) activity and the reverse pattern of lipid peroxidation in Sg and Ce populations, suggesting species-specific differences in antioxidative mechanisms. The Sg individuals developed structural and functional adaptations to protect PSA against drought/salinity stresses (lower leaf chlorophyll, higher flavonoids content, ChlF parameters: Vi, Vj, dV(G)/dto). The Ce populations have higher values of JIP parameters related to the electron transfer site within PSI. Mechanisms of plant species adaptation to industrial areas are crucial for species selection and planning effective reclamation of them. In novel ecosystems of spoil heaps both species responded differently to complex abiotic stresses in comparison to seminatural ones that enable them to gain success on both sites. They can spontaneously colonize such areas, create permanent plant cover, and produce large amounts of biomass. Further research on plant traits response and adaptation to complex environmental stresses on industrial habitats are needed.

关键词： abiotic stresses chlorophyll fluorescence novel ecosystems species response stress markers

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Nutrient uptake efficiency and stoichiometry for different plant functional groups on spoil heap after hard coal mining in Upper Silesia, Poland

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SCIENCE OF THE TOTAL ENVIRONMENT 2024年 924卷 171612页

作者： Wos, Bartlomiej sierka, Edyta Kompala-Baba, Agnieszka Bierza, wojciech Chodak, Marcin Pietrzykowski, Marcin Agr Univ Krakow Fac Forestry Dept Ecol Engn & Forest Hydrol Al 29 Listopada 46 PL-31425 Krakow Poland Univ Silesia Katowice Inst Biol Biotechnol & Environm Protect Jagiellonska 28 PL-40032 Katowice Poland AGH Univ Sci & Technol Dept Environm Management & Protect Al Mickiewicza 30 PL-30059 Krakow Poland

Various plant functional groups (PFGs) used in the reclamation of post-mining heaps may differ in their nutrient uptake efficiency and thus in their effect on the ecosystem development. The effect of PFGs may be additionally modified by the applied reclamation measures such as e.g. topsoiling. In this study we compared the nutrient uptake efficiencies and plant stoichiometry for two PFGs (grasses and forbs) growing on the sites reclaimed by applying topsoil (TS) and unreclaimed sites on carboniferous bare rock (BR) in hard coal spoil heap in Upper Silesia (southern Poland). Basic soil parameters, including pH, texture, soil organic carbon, and nutrients (N, P, K, Ca, and Mg), were measured, and the aboveground plant biomass and nutrient content in plant tissue were determined. Forbs were characterized by a larger biomass and higher nutrient concentrations (except for P) than grasses. The TS treatment supported higher concentrations of N and P in plant tissues but not to the level ensuring more significant primary biomass production. The nutrient concentration and elemental stoichiometry in plant tissue indicated that N was the primary limiting element. However, the major growth limitation for Nfixing forbs was from P. Forbs were much more efficient in nutrient uptake than grasses, independent of the reclamation treatment. Therefore, they stimulate nutrient cycling in the restored ecosystems more than grasses.

关键词： Forbs Grasses Topsoiling Nutrient uptake efficiency Elemental ratios

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Functional ecosystem parameters: Soil respiration and diversity of mite (Acari, Mesostigmata) communities after disturbance in a Late Cambrian bedrock environment

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LAND DEGRADATION & DEVELOPMENT 2022年第17期33卷 3343-3357页

作者： Wozniak, Gabriela Kamczyc, Jacek Bierza, wojciech Blonska, Agnieszka Kompala-Baba, Agnieszka sierka, Edyta Jagodzinski, Andrzej M. Univ Silesia Katowice Inst Biol Biotechnol & Environm Protect Fac Nat Sci Jagiellonska 28 PL-40032 Katowice Poland Poznan Univ Life Sci Fac Forestry & Wood Technol Dept Game Management & Forest Protect Wojska Polskiego 71C PL-60625 Poznan Poland Polish Acad Sci Inst Dendrol Dept Ecol Kornik Poland

We analyzed the changes in ecosystem functions (soil respiration and Mesostigmata mite abundance, species richness and diversity) on various habitats after flooding by highly mineralized and acidic drainage water with fine As-rich pyrite sediments, on a fragment of a natural ecosystem. In total, 177 plots that represented six types of habitats (undisturbed: pine mixed forests, Salix spp. thickets, Juncus effusus communities, wet meadows, and disturbed: bare ground and dead Salix spp. thickets) were sampled twice, in June 2019 and September 2020. Our study revealed that flooding affected essential ecosystem parameters, such as soil Mesostigmata mite abundance, species richness and diversity, and soil respiration, via an extreme decrease of soil pH. In total, 968 mites were collected from pooled data from the two samplings. Mite abundance, species richness and diversity were mainly shaped by habitat type and soil pH, and partially by soil respiration. These parameters were lower in disturbed habitats (bare ground and dead Salix spp. thickets) as compared with undisturbed ones. The highest mean mite density was recorded from mixed forests (4750 +/- 600 ind. m(-2)) and wet meadows (2678 +/- 361 ind. m(-2)), whereas the lowest in bare ground (449 +/- 113 ind. m(-2)) and dead Salix spp. thickets (537 +/- 146 ind. m(-2)). We noticed that mite diversity may be helpful to predict future changes in the physicochemical parameters of soils in disturbed areas. The vegetation patches built up by species adapted to grow in habitats characterized by relatively high metal and metalloid content (J. effusus and Salix spp.) represent an intermediate state of function of ecosystems in the study area.

关键词： arsenic waters biotic and abiotic soil parameters fine As-rich pyrite geological deposits flooding plant communities vegetation types

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Arbuscular mycorrhizal fungi (AMF) root colonization dynamics of Molinia caerulea (L.) Moench. in grasslands and post-industrial sites

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ECOLOGICAL ENGINEERING 2016年第0期95卷 817-827页

作者： Baba, wojciech Blonska, Agnieszka Kompala-Baba, Agnieszka Malkowski, Lukasz Ziemer, Barbara sierka, Edyta Nowak, Teresa Wozniak, Gabriela Besenyei, Lynn Jagiellonian Univ Inst Bot Dept Plant Ecol Lubicz 46 PL-31512 Krakow Poland Univ Silesia Dept Bot & Nat Protect Jagiellonska 28 PL-40032 Katowice Poland Wolverhampton Univ Fac Sci & Engn Wolfruna St Wolverhampton W Midlands England

The aims of this studies were: (i) to examine the influence of heavy metal content (Zn, Cd, Pb, Fe, Cu) and other physico-chemical soil parameters on the level of root colonization of Molinia caerulea and (ii) to relate root colonisation parameters and soil variables to Molinia caerulea abundance in two contrasting habitats (grasslands and heavy metal contaminated sites). The sites differ significantly in terms of bio-available heavy metal contents, particularly Zn (34 times more than grasslands), soil texture, CaCO3, organic matter (LOI%), Mg and nitrate content. Principal Component Analysis showed the strong negative correlations between frequency of mycorrhization (F), arbuscular abundance (A%) and intensity of root cortex colonisation (M%) and concentration of bio-available Zn and Cd. Moreover, no positive correlation between root colonization of Molinia and its abundance was found. The frequency of mycorrhization of root fragments (F%) was only slightly different between these two habitats, whereas the intensity of root cortex colonisation (M%) and relative arbuscular abundance (A%) were significantly lower (3 and 4 times respectively) on the post-industrial sites. The bioavailable Zn content in the substratum of post-industrial sites was strongly negatively correlated with species richness, Shannon diversity index and Evenness. In contrast, these relationships were not statistically significant in grasslands. Based on obtained results we could draw a model of possible relationships between root colonization of Molinia, HM content and Molinia abundance on grasslands and post-industrial sites. Bioavailable Zn content in the soil is a one of main factors influencing the Molinia community diversity. In the grasslands, lower amounts of bioavailable Zn, resulted in higher species richness (R) and species diversity (H) which in turn lead to higher root colonization. On the other hand, on the post-industrial sites, the elevated bioavailable Zn content strongly decreas

关键词： Arbuscular mycorrhizal fungi Heavy metals Contaminated sites Grasses Physico-chemical soil properties Reclamation

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Vegetation diversity on coal mine spoil heaps - how important is the texture of the soil substrate?

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BIOLOGIA 2019年第4期74卷 419-436页

作者： Kompala-Baba, Agnieszka Bierza, wojciech Blonska, Agnieszka sierka, Edyta Magurno, Franco Chmura, Damian Besenyei, Lynn Radosz, Lukasz Wozniak, Gabriela Univ Silesia Katowice Fac Biol & Environm Protect Jagiellonska Str 28 PL-40032 Katowice Poland Univ Bielsko Biala Inst Environm Protect & Engn Willowa 2 PL-43309 Bielsko Biala Poland Univ Wolverhampton Sch Sci Fac Sci & Engn Wulfruna St Wolverhampton WV1 1LY W Midlands England

The relationship between the size of the particle fractions of the soil substrate and the diversity of the spontaneously developing vegetation was investigated on coal mine spoil heaps in Upper Silesia (Southern Poland). The analyses were based on 2567 research plots of developed spontaneous vegetation and their associated soil substrate samples collected from 112 coal mine spoil heaps. For each research plot the prevailing particle size fraction was determined (stones, gravel, sand, silt), the species composition and abundance was recorded and the species richness (S), Shannon-Wiener diversity index (H), Simpson (C) and Evenness (E) indices were used to determine species diversity. From a total of 119 research plots (in all particle size fraction categories), the values of 15 physicochemical properties (pH, electrical conductivity, water holding capacity, moisture, carbon content, total N, available P, Mg and exchange cations Ca, Mg, K, Na, fine particles (%), gravel (%), stone (%)) were obtained to asses their impact on the floristic composition of vegetation patches using Canonical Correspondence Analysis (CCA). Additionally, functional traits of the dominant species of each vegetation patch (life forms, life strategies and socio-ecological groups), were selected to analyse their relation to substrate texture. It was shown that the highest species richness and the highest values for Shannon-Wiener diversity index, as well as Simpson and Evenness indices, were obtained in plots formed on stones. Moreover, the greatest variation in the participation of species representing different habitats, life forms, and life strategies was found on gravelly substrates. Contrary to our expectations, the vegetation diversity (in terms of both species and their functional traits) was not highest in habitats with a high composition of fine size particles.

关键词： Soil texture Vegetation Coal mining post-industrial sites

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