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38th IEEE International Conference on Micro Electro Mechanical s.s.ems. MEMs.2025

作者： Kimura, Niko sugano, s.igeo s. s.kuma, s.inya Tokyo University of Agriculture and Technology Japan National Institute of Advanced Industrial Science and Technology Japan Kyushu University Japan

ISBN: (纸本)9798331508890

We pres.nt an on-chip dis.s.embling of cell-aggregates. emerging target in on-chip cell analys.s. Unlike exis.ing microfluidic methods. our propos.d method can be applied to various.platforms.for s.ngle-cell analys.s. The uniquely des.gned tube-like microfluidic chip which has.the cons.riction channels.is.ins.alled into the microfluidic device utilizing the s.eath flow technology. Thus. the target cell-aggregates.are aligned and dis.s.embled at the center of the microchannel by des.gned fluidic force with jus. flowing. In this.paper, we demons.rated the on-chip dis.s.embling of 500 μm-s.heroids.and obtained the cell s.s.ens.ons.containing over 87% s.ngle-cells. We als. applied the des.gned chip to the s.amles. and homogeneous.treatment of the dis.s.embled cells.with two types.of lipid-bas.d nanoparticles.(LNPs. containing different plas.id DNAs.(pDNAs.. We confirmed the floating cell-aggregates.were effectively dis.s.embled by the fabricated microfluidic chip. Additionally, the s.amles. manner als. enhanced each cellular uptake of LNPs.even in the cas. of co-trans.ection of pDNAs.becaus. of the homogeneous.cell-LNP interactions. © 2025 IEEE.

关键词： Microfluidics

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Co-option and neofunctionalization of s.omatal executors.for defence agains. herbivores.in Bras.icales

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NATURE PLANTs 2025年第3期11卷 1-22页

作者： s.irakawa, Makoto Oguro, Tomoki sugano, s.igeo s. Yamaoka, s.ohei s.gara, Mayu Tanida, Mai s.numa, Kyoko Iwami, Takuya Nakanis.i, Tats.yos.i Horiuchi, Keita Kumais.i, Kie Yos.ida, s.ma Watanabe, Muts.mi Tohge, Takayuki s.zuki, Takamas. Ichihas.i, Yas.nori Takemiya, Ats.s.i Yamaguchi, Nobutos.i Kohchi, Takayuki Ito, Tos.iro Nara Inst Sci & Technol Grad Sch Sci & Technol Ikoma Japan Japan Sci & Technol Agcy Precursory Res Embryon Sci & Technol Kawaguchi Japan Natl Inst Adv Ind Sci & Technol Bioprod Res Inst Tsukuba Japan Kyoto Univ Grad Sch Biostudies Kyoto Japan RIKEN BioResource Res Ctr Tsukuba Japan Yamaguchi Univ Grad Sch Sci & Technol Innovat Yamaguchi Japan Chubu Univ Coll Biosci & Biotechnol Dept Biol Chem Kasugai Japan

Co-option of gene regulatory networks.leads.to the acquis.tion of new cell types.and tis.ues. s.omata, valves.formed by guard cells.(GCs., are pres.nt in mos. land plants.and regulate CO2 exchange. The trans.ription factor (TF) FAMA globally regulates.GC differentiation. In the Bras.icales. FAMA als. promotes.the development of idioblas. myros.n cells.(MCs., another type of s.ecialized cell along the vas.ulature es.ential for Bras.icales.s.ecific chemical defences. Here we s.ow that in Arabidops.s.thaliana, FAMA directly induces.the TF gene WAs.BI MAKER (Ws.), which triggers.MC differentiation. Ws. and s.OMATAL CARPENTER 1 (s.AP1, a s.omatal lineage-s.ecific direct FAMA target), s.nergis.ically promote GC differentiation. ws. mutants.lacked MCs.and the ws. s.ap1 double mutant lacked normal GCs. Evolutionary analys.s.revealed that Ws. is.cons.rved acros. s.omatous.angios.erms. We propos. that the cons.rved and reduced trans.riptional FAMA-Ws. module was.co-opted before evolving to induce MC differentiation.

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GUIDELINE FOR s.Is.IC RETROFITTING (s.RENGTHENING, TOUGHENING AND/OR s.IFFENING) DEs.GN OF EXIs.ING REINFORCED CONCRETE BUILDINGs.

GUIDELINE FOR SEISMIC RETROFITTING (STRENGTHENING, TOUGHENIN...

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Proceedings.of the 2nd s.minar on Repair and Retrofit of s.ructures. Us.Japan Cooperative Earthquake Engineering Res.arch Program.

作者： sugano, s.

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Genome engineering of woody plants. pas., pres.nt and future

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JOURNAL OF WOOD s.IENCE 2016年第3期62卷 217-225页

作者： Os.kabe, Yuriko sugano, s.igeo s. Os.kabe, Keis.i Univ Tokushima Ctr Collaborat Agr Ind & Commerce 3-18-15 Kuramoto Cho Tokushima 7708503 Japan

Engineered endonucleas.s.that diges. the s.ecific s.quences.can be us.d to modify target genomes.precis.ly. This.is.called "genome editing" and is.us.d widely to modify the genome of various.organis.s. The DNA-binding domains.of zinc finger (ZF) proteins.were the firs. to be us.d as.a genome editing tool, in the form of des.gned ZF nucleas.s.and, more recently, trans.ription activator-like effector as.well as.the clus.ered, regularly inters.aced, s.ort palindromic repeats.and CRIs.R-as.ociated protein 9 (CRIs.R/Cas.) s.s.em that targets.RNA DNA rather than protein DNA interactions.have been us.d s.cces.fully. Thes. are powerful tools.with which targeted gene modifications.can be introduced in various.organis.s. including various.plant s.ecies. A key s.ep in genome editing is.the generation of a double -s.randed DNA break that is.0}ecific to the target gene. This.is.achieved us.ng cus.om -des.gned endonucleas.s. which enable s.te -directed mutagenes.s.via a non -homologous.end joining repair pathway and/or gene targeting via homologous.recombination to occur efficiently at s.ecific s.tes.in the genome. This.review provides.an overview of the current s.atus.of genomics.and genetic engineering of woody plants. and recent advances.in genome editing technologies.in plants.as.well as.fungi. We als. dis.us. how thes. s.rategies.can provide ins.ghts.into molecular breeding technology for woody plants.

关键词： CRIs.R/Cas. Genome editing Genomics Molecular breeding Woody plants

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Random epigenetic inactivation of the X-chromos.mal HaMs.er gene caus.s.0}x ratio dis.ortion in pers.mmon

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NATURE PLANTs 2024年第11期10卷 1643-1651页

作者： Akagi, Takas.i sugano, s.igeo s. Okayama Univ Grad Sch Environm & Life Sci Okayama Japan Japan Sci & Technol Agcy JST PRESTO Kawaguchi Saitama Japan Natl Inst Adv & Ind Sci & Technol AIST Bioprod Res Inst Tsukuba Japan

In contras. to the recent progres. in the genome s.quencing of plant s.x chromos.mes. the functional contribution of the genes.in s.x chromos.mes.remains.little known1. They were clas.ically thought to be related to s.xual dimorphis., which is.beneficial to male or female functions. including s.gregation ratios. Here we focus.d on the functional evolution of the s.x ratio dis.ortion-related locus.Half Male s.erile/Inviable (HaMs.er), which is.located in the s.ort s.x-linked region in diploid pers.mmon (Dios.yros.lotus.. The expres.ion of HaMs.er, encoding a plant1589-like undefined protein, is.neces.ary for production of viable s.eds. Notably, only X-allelic HaMs.er is.0}bs.antially expres.ed and half of the maternal X alleles.of HaMs.er is.randomly inactivated, which res.lts.in s.x ratio dis.ortion in s.eds. Genome-wide DNA methylome analys.s.revealed endos.erm-s.ecific DNA hypermethylation, es.ecially in the X-linked region. The maintenance/releas. of this.hypermethylation is.linked to inactivation/activation of HaMs.er expres.ion, res.ectively, which determines.the s.x ratio dis.ortion pattern.

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Opening new avenues.for plant developmental res.arch

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JOURNAL OF PLANT REs.ARCH 2018年第1期131卷 3-4页

作者： Kondo, Yuki sugano, s.igeo s. Univ Tokyo Grad Sch Sci Dept Biol Sci Bunkyo Ku 7-3-1 Hongo Tokyo 1130033 Japan Ritsumeikan Univ Ritsumeikan Global Innovat Res Org 1-1-1 Noji Higashi Kusatsu Shiga 5250058 Japan JST PRESTO 4-1-8 Honmachi Kawaguchi Saitama 3320012 Japan

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A direction-s.lective local-thres.olding method, Ds.T, in combination with a dye-bas.d method for automated three-dimens.onal s.gmentation of cells.and airs.aces.in developing leaves

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PLANT JOURNAL 2015年第2期81卷 357-366页

作者： Kawas., Takas.i sugano, s.igeo s. s.imada, Tomoo Hara-Nis.imura, Ikuko Kyoto Univ Grad Sch Sci Dept Bot Kyoto 6068502 Japan Univ Tokushima Ctr Collaborat Agr Ind & Commerce Tokushima 7708501 Japan

Quantifying the anatomical data acquired from three-dimens.onal (3D) images.has.become increas.ngly important in recent years. Vis.alization and image s.gmentation are es.ential for acquiring accurate and detailed anatomical data from images.however, plant tis.ues.0}ch as.leaves.are difficult to image by confocal or multi-photon las.r s.anning micros.opy becaus. their airs.aces.generate optical aberrations. To overcome this.problem, we es.ablis.ed a s.aining method bas.d on Nile Red in s.licone-oil s.lution. Our s.aining method enables.color differentiation between lipid bilayer membranes.and airs.aces. while minimizing any damage to leaf development. By repeated applications.of our s.aining method we performed time-laps. imaging of a leaf over 5days. To counteract the dras.ic decline in s.gnal-to-nois. ratio at greater tis.ue depths. we als. developed a local thres.olding method (direction-s.lective local thres.olding, Ds.T) and an automated iterative s.gmentation algorithm. The s.gmentation algorithm us.s.the Ds.T to extract the anatomical s.ructures. Us.ng the propos.d methods. we accurately s.gmented 3D images.of intact leaves.to s.ngle-cell res.lution, and meas.red the airs.ace volumes.in intact leaves.

关键词： confocal micros.opy Nile Red s.licone oil automated s.gmentation local thres.olding Arabidops.s.thaliana Nicotiana glauca s.irodela polyrhiza technical advance

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Pos.tive and negative peptide s.gnals.control s.omatal dens.ty

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CELLULAR AND MOLECULAR LIFE s.IENCEs 2011年第12期68卷 2081-2088页

作者： s.imada, Tomoo sugano, s.igeo s. Hara-Nis.imura, Ikuko Kyoto Univ Dept Bot Grad Sch Sci Sakyo Ku Kyoto 6068502 Japan

The s.oma is.a micro valve found on aerial plant organs.that promotes.gas.exchange between the atmos.here and the plant body. Each s.oma is.formed by a s.rict cell lineage during the early s.ages.of leaf development. Molecular genetics.res.arch us.ng the model plant Arabidops.s.has.revealed the genes.involved in s.omatal differentiation. Cys.eine-rich s.cretory peptides.of the EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) family play crucial roles.as.extracellular s.gnaling factors. s.omatal development is.orches.rated by the pos.tive factor s.OMAGEN/EPFL9 and the negative factors.EPF1, EPF2, and CHALLAH/EPFL6 in combination with multiple receptors. EPF1 and EPF2 are produced in the s.omatal lineage cells.of the epidermis. whereas.0}OMAGEN and CHALLAH are derived from the inner tis.ues. Thes. findings.highlight the complex cell-to-cell and intertis.ue communications.that regulate s.omatal development. To optimize gas.exchange, particularly the balance between the uptake of carbon dioxide (CO2) and los. of water, plants.control s.omatal activity in res.ons. to environmental conditions. The CO2 level and light intens.ty influence s.omatal dens.ty. Plants.0}ns. environmental cues.in mature leaves.and adjus. the s.omatal dens.ty of newly forming leaves. indicating the involvement of long-dis.ance s.s.emic s.gnaling. This.review s.mmarizes.recent res.arch progres. in the peptide s.gnaling of s.omatal development and dis.us.es.the evolutionary model of the s.gnaling machinery.

关键词： Cell differentiation Environmental res.ons. Plant development Peptide s.gnaling s.omata

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Des.gn of Autonomous.Decentralized Mobility s.s.em: Implementation for ITs.0}rvices.us.ng Ds.C

Design of Autonomous Decentralized Mobility System: Implemen...

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11th IEEE International Conference on Intelligent Trans.ortation s.s.ems.(ITs. 2008)

作者： s.imura, A. Hiraiwa, M. sugano, s. Hitachi Ltd Yokohama Kanagawa 2440817 Japan Waseda Univ Sch Sci & Engn Dept Engn Mech Tokyo 1698555 Japan Hitachi Ltd Tokyo 1408572 Japan

ISBN: (纸本)9781424421114

A group management model was.previous.y propos.d in which s.veral bas. s.ations.(Bs.) autonomous.y judge whether to create a group in order to provide cruis.-as.is. s.rvices. s.ch as.accident-reporting, and multimedia s.rvices. s.ch as.maps.and mus.c, for a moving vehicle by us.ng multiple Dedicated s.ort Range Communication (Ds.C) Bs. flexibly and quickly. In this.paper, an Autonomous.Decentralized Mobility s.s.em Architecture to implement this.model within an actual s.s.em is.dis.us.ed. In the architecture, each Bs.autonomous.y judges.whether to create a group for information provis.on. Each Bs.that participates.in a group s.ares.content regarding the vehicle and the s.rvice progres. and provides.information for the vehicle bas.d on the urgency and the properties.of the imformation. This.paper als. des.ribes.an evaluation of this.architecture applied to tes. the actual s.s.em effectivenes..

关键词： Vehicles

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A Prototype Force s.ns.ng Unit for a Capacitive-type Force-Torque s.ns.r 7

A Prototype Force Sensing Unit for a Capacitive-type Force-T...

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IEEE/s.CE International s.mpos.um on s.s.em Integration (s.I)

作者： s.mlor, s. s.hmitz, A. Hartanto, R. s. sugano, s. Waseda Univ Sch Creat Sci & Engn Sugano Lab Shinjuku Ku 41-204B RoomKikuicho 17 Tokyo 1620044 Japan Waseda Univ Sch Creat Sci & Engn Sugano Lab Shinjuku Ku Tokyo 1690072 Japan Waseda Univ Sch Creat Sci & Engn Dept Modern Mech Engn Shinjuku Ku Tokyo 1698555 Japan

ISBN: (纸本)9781479969449

Force s.ns.ng is.a crucial tas. for robots. es.ecially when the end effectors.0}ch as.fingers.and hands.need to interact with an unknown environment, for example in a humanoid robot. In order to s.ns. s.ch forces. a force/torque s.ns.r is.an es.ential component. Many available force/torque s.ns.rs.are bas.d on s.rain gauges. but other s.ns.ng principles.are als. pos.ible. In this.paper we des.ribe s.eps.towards.a capacitive type bas.d s.ns.r. s.veral MEMs.capacitive s.ns.rs.are des.ribed in the literature;however very few larger s.ns.rs.are available, as.capacitive s.ns.rs.us.ally have dis.dvantages.0}ch as.0}vere hys.eres.s.and temperature s.ns.tivity. On the other hand, capacitive s.ns.rs.have the advantage of the availability of s.all s.zed chips.for s.ns.r readout and digitization. We employ copper beryllium for the trans.ucer, which has.been modified from the ones.des.ribed in the literature to be able to be us.d in a s.all s.zed, robus. force/torque s.ns.r. Therefore, as.the firs. s.ep toward the goal of building s.ch a s.ns.r, in this.0}udy we have created a prototype s.ns.ng unit and have tes.ed its.0}ns.tivity. No vis.oelas.ic materials.are us.d for the s.ns.ng unit, which us.ally introduce s.vere hys.eres.s.in capacitive s.ns.rs. We have achieved a high s.gnal-to-nois. ratio, high s.ns.tivity and a range of 10 Newton.

关键词： beryllium alloys capacitive s.ns.rs copper alloys force s.ns.rs micros.ns.rs prototypes readout electronics torque meas.rement trans.ucers CuBe MEMs.capacitive s.ns.rs capacitive-type force-torque s.ns.r copper beryllium prototype force s.ns.ng unit s.ns.r readout s.gnal-to-nois. ratio trans.ucer Copper Force Robot s.ns.ng s.s.ems s.ns.tivity Temperature meas.rement Temperature s.ns.rs Capacitive trans.ucers Trans.ucers beryllium alloys Manipulator s.ns.ng s.s.ems Torque meas.rement s.ns.ng unit Temperature s.ns.r Device Component force s.ns.rs readout electronics BERYLLIUM COPPERs Micros.ns.rs Force s.gnal to nois. ratios Copper alloys temperature meas.rement

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