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A Highly Efficient BODIPY Based Turn-off Fluorescent Probe for Detecting Cu²⁺

JOURNAL OF FLUORESCENCE 2020年第4期30卷 883-890页

作者： Sun, Ruopei Wang, Lusheng Jiang, Chao du, Ziyao Chen, suwen Wu, Wangsuo Lanzhou Univ Sch Nucl Sci & Technol 222 Tianshui South Rd Lanzhou Gansu Peoples R China

Based on boron-dipyrromethene (BODIPY), taking 2-hydroxy-N-(2-hydroxyphenyl)benzamide as recognition site, a new fluorescent probe HHPBA-BODIPY aimed at sensitively detecting Cu ions was designed, synthesized and *** emission spectra of HHPBA-BODIPY exhibited an intensive green fluorescence around 510 nm, with a maximum absorption near 500 nm. When Cu2+ ions are present, the fluorescence at 510 nm can be quenched with a good linearity between the copper ion concentrationand the fluorescence intensity and the detection limit is 0.35 mu M. HHPBA-BODIPY is also selective toward Cu2+, while other metal ions show no interfere except Fe3+ and Cr3+ ions. In addition, HHPBA-BODIPY also proved efficient to detect Cu2+ in water samples which offers the possibility to detect trace amount of Cu2+ for environmental monitoring. Copper ions;BODIPY;fluorescent probe.

关键词： BODIPY Fluorescent probe Copper ion detection Cu2+

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Role of tumour-derived exosomes in metastasis

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BIOMEDICINE & PHARMACOTHERAPY 2022年第0期147卷 112657-112657页

作者： Bai, suwen Wei, Yuan Liu, Rong Xu, Ruihuan Xiang, Li du, Juan Chinese Univ Hong Kong Longgang Dist Peoples Hosp Shenzhen Shenzhen 518172 Guangdong Peoples R China Chinese Univ Hong Kong Affiliated Hosp 2 Shenzhen 518172 Guangdong Peoples R China Anhui Med Univ Sch Basic Med Sci 81 Meishan Rd Hefei 230032 Anhui Peoples R China Chinese Univ Hong Kong Sch Med 2001 Longxiang Rd Shenzhen 518172 Guangdong Peoples R China

Tumour-derived exosomes (TDEs) are actively produced and released by tumour cells and carry messages from tumour cells to normal or abnormal cells residing at close or distant sites. TDEs participate in every process of tumour metastasis. However, the occurrence and development of tumours depend on the specific functions acquired by tumour cells on the primary and metastatic foci. In this review, we discussed that TDEs regulate the initial mechanism of metastasis, the formation of a pre-metastatic niche, immunosuppression and angiogenesis. In addition, we investigated the signalling pathways and effective components of TDEs and discussed that inhibition of exosomes can inhibit tumour progression. Finally, we discussed the application and future development of TDEs. An understanding of several molecular players and processes involved in metastasis can lead to the development of effective, targeted approaches to prevent metastasis and treat cancer.

关键词： Angiogenesis Extracellular matrix Epithelial -mesenchymal transition Immunosuppression Pre-metastatic niche Tumour-derived exosomes

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Update on histone deacetylase inhibitors in peripheral T-cell lymphoma (PTCL)

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CLINICAL EPIGENETICS 2023年第1期15卷 124-124页

作者： Lu, Guang Jin, Shikai Lin, suwen Gong, Yuping Zhang, Liwen Yang, Jingwen Mou, Weiwei du, Jun Sichuan Univ West China Hosp Dept Hematol Chengdu 610041 Sichuan Peoples R China Shengli Oilfield Cent Hosp Dept Hematol Dongying 257034 Shandong Peoples R China Shanghai Jiao Tong Univ Sch Med Dept Clin Med Shanghai 200025 Peoples R China Shenzhen Peking Univ Hong Kong Univ Sci & Technol Clin Res Inst Med Ctr Shenzhen 518036 Guangdong Peoples R China Shengli Oilfield Cent Hosp Dept Pediat Dongying 257034 Shandong Peoples R China Shanghai Jiao Tong Univ Renji Hosp Sch Med Dept Hematol Shanghai 200127 Peoples R China

Peripheral T-cell lymphomas (PTCLs) are a group of highly aggressive malignancies with generally poor prognoses, and the first-line chemotherapy of PTCL has limited efficacy. Currently, several novel targeted agents, including histone deacetylase inhibitors (HDACis), have been investigated to improve the therapeutic outcome of PTCLs. Several HDACis, such as romidepsin, belinostat, and chidamide, have demonstrated favorable clinical efficacy and safety in PTCLs. More novel HDACis and new combination therapies are undergoing preclinical or clinical trials. Mutation analysis based on next-generation sequencing may advance our understanding of the correlation between epigenetic mutation profiles and relevant targeted therapies. Multitargeted HDACis and HDACi-based prodrugs hold promising futures and offer further directions for drug design.

关键词： Histone deacetylase HDACi PTCL Romidepsin Belinostat Chidamide

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Exocyst controls exosome biogenesis via Rab11a

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MOLECULAR THERAPY NUCLEIC ACIDS 2022年第0期27卷 535-546页

作者： Bai, suwen Hou, Wenxuan Yao, Yanheng Meng, Jialin Wei, Yuan Hu, Fangfang Hu, Xianyu Wu, Jing Zhang, Ning Xu, Ruihuan Tian, Faqing Wang, Benguo Liao, Hailan du, Yinan Fang, Haoshu He, Wei Liu, Yehai Shen, Bing du, Juan Chinese Univ Hong Kong Longgang Dist Peoples Hosp Shenzhen Shenzhen 518172 Guangdong Peoples R China Chinese Univ Hong Kong Affiliated Hosp 2 Shenzhen 518172 Guangdong Peoples R China Anhui Med Univ Sch Basic Med Sci 81 Meishan Rd Hefei 230032 Anhui Peoples R China Anhui Med Univ Dept Urol Affiliated Hosp 1 Inst UrolAnhui Prov Key Lab Genitourinary Dis Hefei 230022 Peoples R China Anhui Med Univ Dept Gen Surg Affiliated Hosp 1 Hefei 230022 Anhui Peoples R China Anhui Med Univ Dept Otolaryngol Affiliated Hosp 1 Hefei 230022 Anhui Peoples R China Chinese Univ Hong Kong Sch Med 2001 Longxiang Rd Shenzhen 518172 Guangdong Peoples R China

Tumor cells actively release large quantities of exosomes, which pivotally participate in the regulation of cancer biology, including head and neck cancer (HNC). Exosome biogenesis and release are complex and elaborate processes that are considered to be similar to the process of exocyst-mediated vesicle delivery. By analyzing the expression of exocyst subunits and their role in patients with HNC, we aimed to identify exocyst and its functions in exosome biogenesis and investigate the molecular mechanisms underlying the regulation of exosome transport in HNC cells. We observed that exocysts were highly expressed in HNC cells and could promote exosome secretion in these cells. In addition, downregulation of exocyst expression inhibited HN4 cell proliferation by reducing exosome secretion. Interestingly, immunofluorescence and electron microscopy revealed the accumulation of multivesicular bodies (MVBs) after the knockdown of exocyst. Autophagy, the major pathway of exosome degradation, is not activated by this intracellular accumulation of MVBs, but these MVBs are consumed when autophagy is activated under the condition of cell starvation. Rab11a, a small GTPase that is involved in MVB fusion, also interacted with the exocyst. These findings suggest that the exocyst can regulate exosome biogenesis and participate in the malignant behavior of tumor cells.

关键词： exocyst exosome biogenesis head and neck cancer Rab11a multivesicular bodies intraluminal vesicles

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The role of transient receptor potential channels in metastasis

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BIOMEDICINE & PHARMACOTHERAPY 2023年 158卷 114074-114074页

作者： Bai, suwen Wei, Yuan Liu, Rong Chen, Yuhua Ma, Wanling Wang, Minghua Chen, Li Luo, Yumei du, Juan Chinese Univ Hong Kong Longgang Dist Peoples Hosp Shenzhen Shenzhen Guangdong Peoples R China Chinese Univ Hong Kong Affiliated Hosp 2 Sch Med Shenzhen Guangdong Peoples R China Anhui Med Univ Sch Basic Med Sci 81 Meishan Rd Hefei 230032 Anhui Peoples R China Sun Yat sen Univ Affiliated Hosp 7 Dept Obstet & gynecol Zhenyuan Rd Shenzhen 518107 Guangdong Peoples R China Chinese Univ Hong Kong Ciechanover Inst Precis & Regenerat Med Sch Med Shenzhen 518172 Guangdong Peoples R China Chinese Univ Hong Kong Longgang Dist Peoples Hosp Shenzhen Shenzhen 518172 Guangdong Peoples R China Chinese Univ Hong Kong Affiliated Hosp 2 Sch Med Shenzhen 518172 Guangdong Peoples R China Chinese Univ Hong Kong Sch Med 2001 Longxiang Rd Shenzhen 518172 Guangdong Peoples R China

Metastasis is the hallmark of failed tumor treatment and is typically associated with death due to cancer. Transient receptor potential (TRP) channels affect changes in intracellular calcium concentrations and participate at every stage of metastasis. Further, they increase the migratory ability of tumor cells, promote angiogenesis, regulate immune function, and promote the growth of tumor cells through changes in gene expression and function. In this review, we explore the potential mechanisms of action of TRP channels, summarize their role in tumor metastasis, compile inhibitors of TRP channels relevant in tumors, and discuss current challenges in research on TRP channels involved in tumor metastasis.

关键词： Transient Receptor Potential Channels Metastasis Epithelial-mesenchymal transition Angiogenesis Immune Modulation

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Multi-task unscented Kalman inversion (MUKI): a derivative-free joint inversion framework and its application to joint inversion of geophysical data

arXiv

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arXiv 2022年

作者： Wang, Longlong Chen, Yun Liu, Youshan du, Nanqiao Li, Wei suwen, Junliu State Key Laboratory of Lithospheric Evolution Institute of Geology and Geophysics Chinese Academy of Sciences China College of Earth and Planetary Sciences University of Chinese Academy of Sciences Beijing China CAS Center for Excellence in Deep Earth Science Guangzhou China Department of Earth Science University of Toronto Toronto Canada Hubei Subsurface Multi-Scale Imaging Key Laboratory Institute of Geophysics and Geomatics China University of Geosciences Wuhan China Key Laboratory of Earth and Planetary Physics Institute of Geology and Geophysics Chinese Academy of Sciences Beijing China

In the geophysical joint inversion, the gradient and Bayesian Markov Chain Monte Carlo (MCMC) sampling-based methods are widely used owing to their fast convergences or global optimality. However, these methods either require the computation of gradients and easily fall into local optimal solutions, or cost much time to carry out the millions of forward calculations in a huge sampling space. Different from these two methods, taking advantage of the recently developed unscented Kalman method in computational mathematics, we extend an iterative gradient-free Bayesian joint inversion framework, i.e., Multitask unscented Kalman inversion (MUKI). In this new framework, information from various observations is incorporated, the model is iteratively updated in a derivative-free way, and a Gaussian approximation to the posterior distribution of the model parameters is obtained. We apply the MUKI to the joint inversion of receiver functions and surface wave dispersion, which is well-established and widely used to construct the crustal and upper mantle structure of the earth. Based on synthesized and real data, the tests demonstrate that MUKI can recover the model more efficiently than the gradient-based method and the Markov Chain Monte Carlo method, and it would be a promising approach to resolve the geophysical joint inversion problems. Copyright © 2022, The Authors. All rights reserved.

关键词： Surface waves

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Endothelial progenitor cell-derived exosomes ameliorate endothelial dysfunction in a mouse model of diabetes

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BIOMEDICINE & PHARMACOTHERAPY 2020年 131卷 110756-110756页

作者： Bai, suwen Yin, Qianqian Dong, Tao Dai, Fang Qin, Ying Ye, Li du, Juan Zhang, Qiu Chen, Hongbo Shen, Bing Anhui Med Univ Sch Basic Med Sci Hefei 230032 Anhui Peoples R China Anhui Med Univ Maternal & Child Hlth Hosp Dept Obstet & Gynecol 15 Yimin Rd Hefei 230000 Anhui Peoples R China Anhui Med Univ Affiliated Hosp 1 Dept Endocrinol Hefei 230022 Anhui Peoples R China Anhui Med Univ Affiliated Hosp 2 Dept Endocrinol Hefei 230022 Anhui Peoples R China Anhui Med Univ Affiliated Hosp 1 Dept Neurol Hefei 230032 Anhui Peoples R China

Atherosclerosis is a serious cardiovascular complication of diabetes characterized by inflammation and endothelial damage. Indeed, dysfunction of the endothelium is considered an early marker of atherosclerosis. Endothelial progenitor cells (EPCs) are prerequisites for blood vessels lined with endothelial cells (ECs), which produce many factors to regulate blood vessel function. Importantly, EPCs also repair some dysfunctions in ECs. Exosomes have been associated with the occurrence and development of disease. Here, we analyzed the microRNAs (miRNAs) contained in exosomes derived from EPCs by using next-generation sequencing. We found that most of the top 10 highest expressed miRNAs in these exosomes were related to atherosclerosis. In a mouse model of atherosclerotic diabetes, treatment with EPC-derived exosomes significantly reduced the production of diabetic atherosclerotic plaques and inflammatory factors. In an in vitro assay examining the contractility of the thoracic aorta from these mice, the addition of EPC-derived exosomes significantly ameliorated the observed endothelium-dependent contractile dysfunction. Taken together, these results indicated that EPC-derived exosomes ameliorated atherosclerotic endothelial dysfunction in a mouse model of atherosclerotic diabetes. Thus, the present study provides a potential therapeutic application of EPC-derived exosomes in cardiovascular disease.

关键词： endothelial progenitor cell exosomes endothelium diabetes microRNAs next-generation sequencing

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miRNA-221-3p in Endothelial Progenitor Cell-Derived Exosomes Accelerates Skin Wound Healing in Diabetic Mice

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DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY 2020年 13卷 1259-1270页

作者： Xu, Juan Bai, suwen Cao, Yadi Liu, Lei Fang, Yang du, Juan Luo, Li Chen, Mingwei Shen, Bing Zhang, Qiu Anhui Med Univ Affiliated Hosp 1 Dept Endocrinol 218 Jixi Rd Hefei 230022 Anhui Peoples R China Anhui Med Univ Sch Basic Med Sci Hefei 230032 Anhui Peoples R China

Background: Patients with diabetic cutaneous ulcers experience financial burden and a lower quality of life and life expectancy. Endothelial progenitor cell (EPC)-derived exosomes facilitate skin wound healing by positively modulating vascular endothelial cell function. Exosomes play their important regulatory role through microRNA (miRNA). We explored the potential role and molecular mechanisms of miRNA in EPC-derived exosome healing of diabetic skin wounds. Methods: Exosomes were isolated from the media of EPCs derived from mice bone marrow. High-throughput sequencing was used to detect the expression of exosome miRNA, and miRNA target genes were predicted using online databases. A diabetic mouse skin wound model was established, and wounds were treated with exosomes, miRNA-221-3p, or phosphate-buffered saline. Results: Exosomes from EPCs accelerated skin wound healing in both control and diabetic mice. High-throughput sequencing showed that miRNA-221-3p was highly expressed in EPC-derived exosomes. Skin wound healing in control and diabetic mice was significantly enhanced by EPC-derived exosomes and miRNA-221-3p administration Immunohistochemical analyses showed that EPC-derived exosomes and miRNA-221-3p increased protein expression levels of the angiogenesis-related factors VEGF, CD31 and cell proliferation marker Ki67. Bioinformatics analyses indicated that miRNA-221-3p may be involved in the AGE-RAGE signaling pathway in diabetic complications, cell cycle, and the p53 signaling pathway. Conclusion: We concluded that miRNA-221-3p is one of the high-expressed miRNAs in EPC-derived exosomes and promoted skin wound healing in diabetic mice. The finding uncovers the molecular mechanism of EPC-derived exosomes and provides a potential novel approach to the clinical treatment of diabetic skin wounds.

关键词： endothelial progenitor cells exosome diabetes miRNA-221-3p wound healing

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从《树林风景》文本结构看奥康纳的女性观

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淮海工学院学报（人文社会科学版） 2017年第11期15卷 42-44页

作者：杜素文江苏财会职业学院基础部江苏连云港222061

《树林风景》是美国知名女作家弗兰纳里·奥康纳创作的短篇小说,描述了一对祖孙因家庭生活矛盾冲突最终酿成血案的悲剧。以外孙女玛丽为切入点,围绕着她与外祖父方琼先生的矛盾冲突对该短篇小说进行文本精读,分析和评价了奥康纳的... 详细信息

《树林风景》是美国知名女作家弗兰纳里·奥康纳创作的短篇小说,描述了一对祖孙因家庭生活矛盾冲突最终酿成血案的悲剧。以外孙女玛丽为切入点,围绕着她与外祖父方琼先生的矛盾冲突对该短篇小说进行文本精读,分析和评价了奥康纳的女性观。

关键词：弗兰纳里·奥康纳短篇小说女性观《树林风景》文本结构

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奥康纳短篇小说多符号特征创作风格研究

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淮海工学院学报（人文社会科学版） 2017年第9期15卷 45-47页

作者：杜素文江苏财会职业学院基础部江苏连云港222061

美国南方文学是美国文学史的一个重要组成部分,培养了大批优秀的作家,女作家弗兰纳里·奥康纳就是其中优秀的代表。奥康纳的小说因其与许多流行文学和文化背道而驰而散发着独特的魅力,吸引了广大读者和文学评论家的目光,有关她本人... 详细信息

美国南方文学是美国文学史的一个重要组成部分,培养了大批优秀的作家,女作家弗兰纳里·奥康纳就是其中优秀的代表。奥康纳的小说因其与许多流行文学和文化背道而驰而散发着独特的魅力,吸引了广大读者和文学评论家的目光,有关她本人和其作品的研究经久不衰。在查阅大量史料的基础上,对奥康纳的短篇小说进行深入研究,从多符号性这一特点上解读其短篇小说的创作风格。

关键词：弗兰纳里·奥康纳短篇小说创作风格多符号特征

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