检索结果-内蒙古大学图书馆

Integrated analyses of multi-omic data derived from paired primary lung cancer and brain metastasis reveal the metabolic vulnerability as a novel therapeutic target

引用

GENOME MEDICINE 2024年第1期16卷 1-30页

作者： Duan, Hao ren, Jianlan Wei, Shiyou Yang, Zhenyu Li, Chuan Wang, Zhenning Li, Meichen Wei, Zhi Liu, Yu Wang, Xiuqi Lan, Hongbin Zeng, Zhen Xie, Maodi Xie, Yuan Wu, suwen Hu, Wanming Guo, Chengcheng Zhang, Xiangheng Liang, Lun Yu, Chengwei Mou, Yanhao Jiang, Yu Li, Houde Sugarman, Eric Deek, Rebecca A. Chen, Zexin Li, Tao Chen, Yaohui Yao, Maojin Chen, Likun Liu, Lunxu Zhang, Gao Mou, Yonggao Sun Yat Sen Univ Canc Ctr Guangdong Prov Clin Res Ctr Canc State Key Lab Oncol South ChinaCanc Ctr Guangzhou 510060 Peoples R China New Jersey Inst Technol Ying Wu Coll Comp Dept Comp Sci Newark NJ 07102 USA Sichuan Univ West China Hosp Inst Thorac Oncol Dept Thorac Surg Chengdu 610041 Peoples R China Southern Med Univ Affiliated Hosp 10 Dongguan Peoples Hosp Dept Neurosurg Dongguan 523018 Peoples R China Sun Yat Sen Univ Guangdong Prov Clin Res Ctr Canc Dept Med Oncol State Key Lab Oncol South ChinaCanc Ctr Guangzhou 510060 Peoples R China Univ Hong Kong Prince Philip Dent Hosp Fac Dent Hong Kong Peoples R China Sichuan Univ West China Hosp Natl Local Joint Engn Res Ctr Translat Med Anesthe Dept AnesthesiolLab Mitochondrial Metab & Periope Chengdu 610041 Peoples R China Sun Yat Sen Univ Canc Ctr Guangdong Prov Clin Res Ctr Canc State Key Lab Oncol South ChinaCanc Ctr Guangzhou 510060 Peoples R China Sun Yat Sen Univ Guangdong Prov Clin Res Ctr Canc Dept Pathol State Key Lab Oncol South ChinaCanc Ctr Guangzhou 510060 Peoples R China Guangxi Med Univ Affiliated Hosp 1 Dept Neurosurg Nanning 530021 Peoples R China Sun Yat Sen Univ Lingnan Hosp Affiliated Hosp 3 Dept Neurosurg Guangzhou 510530 Peoples R China Sichuan Univ West China Hosp Dept Neurosurg Chengdu 610041 Peoples R China Philadelphia Coll Osteopath Med Philadelphia PA 19131 USA Univ Penn Perelman Sch Med Dept Biostat Epidemiol & Informat Philadelphia PA 19104 USA Guangdong Res Ctr Organoid Engn & Technol Guangzhou 510535 Peoples R China Guangzhou Med Univ Affiliated Hosp 1 Guangzhou Inst Resp Dis State Key Lab Resp Dis Guangzhou 510182 Peoples R China

BackgroundLung cancer brain metastases (LC-BrMs) are frequently associated with dismal mortality rates in patients with lung cancer;however, standard of care therapies for LC-BrMs are still limited in their efficacy. A deep understanding of molecular mechanisms and tumor microenvironment of LC-BrMs will provide us with new insights into developing novel therapeutics for treating patients with ***, we performed integrated analyses of genomic, transcriptomic, proteomic, metabolomic, and single-cell RNA sequencing data which were derived from a total number of 154 patients with paired and unpaired primary lung cancer and LC-BrM, spanning four published and two newly generated patient cohorts on both bulk and single cell *** uncovered that LC-BrMs exhibited a significantly greater intra-tumor heterogeneity. We also observed that mutations in a subset of genes were almost always shared by both primary lung cancers and LC-BrM lesions, including TTN, TP53, MUC16, LRP1B, RYR2, and EGFR. In addition, the genome-wide landscape of somatic copy number alterations was similar between primary lung cancers and LC-BrM lesions. Nevertheless, several regions of focal amplification were significantly enriched in LC-BrMs, including 5p15.33 and 20q13.33. Intriguingly, integrated analyses of transcriptomic, proteomic, and metabolomic data revealed mitochondrial-specific metabolism was activated but tumor immune microenvironment was suppressed in LC-BrMs. Subsequently, we validated our results by conducting real-time quantitative reverse transcription PCR experiments, immunohistochemistry, and multiplexed immunofluorescence staining of patients' paired tumor specimens. Therapeutically, targeting oxidative phosphorylation with gamitrinib in patient-derived organoids of LC-BrMs induced apoptosis and inhibited cell proliferation. The combination of gamitrinib plus anti-PD-1 immunotherapy significantly improved survival of mice bearing LC-BrMs. Patients with a hig

关键词： Lung cancer brain metastases (LC-BrMs) Mitochondrial-specific metabolism Tumor immune microenvironment

来源：评论

学校读者我要写书评

暂无评论

Pressure-enhanced thermopower of ionic thermoelectric gelatin for identifying materials with different thermal conductivities

引用

Nano Energy 2025年 142卷

作者： Wang, Jiabin Xu, suwen Qian, Weiqi Hasan, Md Al Mahadi ren, Zilin Yang, Ya Education Department of Guangxi Zhuang Autonomous Region School of Physical Science & Technology Guangxi University Nanning530004 China CAS Center for Excellence in Nanoscience Beijing Key Laboratory of Micro-nano Energy and Sensor Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences Beijing101400 China School of Nanoscience and Technology University of Chinese Academy of Sciences Beijing100049 China

As the human body's most vital environmental interface, the skin integrates diverse sensory receptors enabling simultaneous perception of multiple stimuli through multisensory processing. However, the realization of multifunctional sensing system on robots or prosthetics remains a huge challenge. Here we report a multifunctional tactile sensor fabricated from ionic thermoelectric gelatin that achieves simultaneous pressure and temperature detection. The thermoelectric effect of this ionic thermoelectric gelatin is mainly achieved through the synergy of the thermodiffusion effects and the thermogalvanic effect of ions. In addition, by applying pressure, the thermoelectric effect of this ionic thermoelectric gelatin can be changed (the thermopower of the ionic thermoelectric gelatin increases from 1.21 mV/K to 1.67 mV/K, representing a 38 % increase), so as to enable the sensor to sense external forces. By comparing the thermoelectric effect driven by temperature difference and the thermoelectric effect driven by pressure-temperature difference, there is a difference in the voltage response time. This allows for the decoupling of signals at the backend with only a simple algorithm. Using this sensor, we achieved a 95.31 % accuracy rate in identifying different materials. The multifunctional tactile sensor can promote the development of robots and better assist the disabled in restoring their sensory abilities. © 2025 Elsevier Ltd

关键词： Positive temperature coefficient

来源：评论

学校读者我要写书评

暂无评论

水下井口系统拉伸与弯曲测试工装设计与分析

引用

石油机械 2023年第2期51卷 56-63页

作者：赵苏文赵维青李健邓玉明任志泉唐龙张晏铭中海油能源发展股份有限公司工程技术深水钻采技术公司中国石油大学(华东)海洋油气装备与安全技术研究中心

水下井口系统在深水油气开采作业期间承受复杂载荷的作用,极易发生强度破坏和结构失效。目前主要通过数值模拟的手段对水下井口系统进行分析,无法准确评估水下井口在工程应用时的安全性,研制水下井口系统的专用测试设备并分析其试验过... 详细信息

水下井口系统在深水油气开采作业期间承受复杂载荷的作用,极易发生强度破坏和结构失效。目前主要通过数值模拟的手段对水下井口系统进行分析,无法准确评估水下井口在工程应用时的安全性,研制水下井口系统的专用测试设备并分析其试验过程中的结构稳定性具有重要意义。根据水下井口系统实际装配结构,自主设计了水下井口承受悬挂、拉伸、弯曲载荷下的专用测试工装。为论证测试工装结构在测试试验过程中是否会发生强度破坏,基于ANSYS Worbench建立了3种测试工装的有限元模型,分析得到不同工况下测试工装应力响应云图。计算结果表明:悬挂载荷测试时,测试工装最大应力为597.50 MPa;弯曲载荷测试时,测试工装最大应力为349.34 MPa;拉伸载荷测试时,测试工装最大应力为179.14 MPa。各部件应力均未超出材料屈服强度,能够保证测试试验的稳定运行。研究结果可为水下井口系统测试工装的研究设计提供参考。

关键词：水下井口载荷测试工装有限元分析强度分析 ANSYS Workbench

来源：评论

学校读者我要写书评

暂无评论

Physical interaction of tropomyosin 3 and STIM1 regulates vascular smooth muscle contractility and contributes to hypertension

引用

BIOMEDICINE & PHARMACOTHERAPY 2021年 134卷 111126-111126页

作者： Xu, Fangfang Zhu, Jinhang Chen, Ye He, Ke Guo, Jizheng Bai, suwen Zhao, ren Du, Juan Shen, Bing Anhui Med Univ Sch Basic Med Sci 81 Mei Shan Rd Hefei 230032 Anhui Peoples R China Anhui Med Univ Affiliated Hosp 1 Dept Cardiol Hefei 230032 Anhui Peoples R China

Scope: Tropomyosin (TPM), an actin-binding protein widely expressed across different cell types, is primarily involved in cellular contractile processes. We investigated whether TPM3 physically and functionally interacts with stromal interaction molecule 1 (STIM1) to contribute to vascular smooth muscle cell (VSMC) contraction, store-operated calcium entry (SOCE), and high-salt intake-induced hypertension in rats. Methods and results: Analysis of a rat RNA-seq data set of 80 samples showed that the STIM1 and Tpm3 transcriptome expression pattern is highly correlated, and co-immunoprecipitation results indicated that TPM3 and STIM1 proteins physically interacted in rat VSMCs. Immunohistochemical data displayed obvious co-localization of TPM3 and STIM1 in rat VSMCs. Knockdown of TPM3 or STIM1 in VSMCs with specific small interfering RNA significantly suppressed contractions in tension measurement assays and decreased SOCE in calcium assays. Rats fed a high-salt diet for 4 weeks had significantly higher systolic blood pressure than controls, with significantly increased contractility and markedly increased TPM3 and STIM1 expression levels in the mesenteric resistance artery (shown by tension measurements and immunoblotting, respectively). Additionally, high salt environment in vitro induced significant enhancement of TPM3 and STIM1 expression levels in VSMCs. Conclusions: We showed for the first time that TPM3 and STIM1 physically and functionally interact to contribute to VSMC contraction, SOCE, and high-salt intake-induced hypertension. Our findings provide mechanistic insights and offer a potential therapeutic target for high-salt intake-induced hypertension.

关键词： TPM3 STIM1 High-salt intake Vascular smooth muscle cell Store-operated calcium entry Vasoconstriction

来源：评论

学校读者我要写书评

暂无评论

上海市2010—2020年健康人群麻疹和风疹及流行性腮腺炎病毒抗体水平研究

引用

中华预防医学杂志 2022年第8期56卷 1095-1100页

作者：杨玉颖汤素文唐伟范佳磊李智杨佳炜任佳李崇山上海市疾病预防控制中心病原生物检定所上海200336 上海市闵行区疾病预防控制中心传染病防治科上海201101

目的了解2010—2020年上海市健康人群麻疹、风疹及流行性腮腺炎(流腮)病毒的血清IgG抗体水平,分析不同年龄组人群的抗体变化趋势。方法于2010年1月至2020年12月,将上海市10828名未患麻疹、风疹和流腮的健康人群纳入研究,分为0~7月龄、8... 详细信息

目的了解2010—2020年上海市健康人群麻疹、风疹及流行性腮腺炎(流腮)病毒的血清IgG抗体水平,分析不同年龄组人群的抗体变化趋势。方法于2010年1月至2020年12月,将上海市10828名未患麻疹、风疹和流腮的健康人群纳入研究,分为0~7月龄、8~11月龄、1~2、3~4、5~6、7~9、10~14、15~19、20~29、30~39、40~49、50岁及以上12个年龄组。采集血清样本后,采用ELISA法检测血清中麻疹、风疹及流腮病毒IgG抗体水平,并分析抗体阳性率和抗体水平的差异。结果10828名对象年龄M(Q1,Q3)为8岁(9月龄,20岁);男性占48.34%(5234/10828),女性占50.92%(5514/10828),性别信息不详者占0.74%(80/10828);MMR免疫史不详者占27.03%(2927/10828)。10828名对象麻疹、风疹及流腮病毒IgG抗体总阳性率分别为76.78%、64.46%和64.29%,各年份麻疹、风疹及流腮病毒血清IgG抗体阳性率差异均有统计学意义(χ^(2)_(麻疹)=1049.50,P<0.001;χ^(2)_(风疹)=1103.11,P<0.001;χ^(2)_(流腮)=782.59,P<0.001)。麻疹、风疹及流腮病毒IgG抗体GMC分别为541.45 mIU/ml、31.76 IU/ml和133.73 U/ml,各年份麻疹、风疹及流腮病毒血清IgG抗体GMC差异均具有统计学意义(F麻疹=180.74,P<0.001;F风疹=189.95,P<0.001;F_(流腮)=122.40,P<0.001)。麻疹病毒抗体阳性率总体高于风疹和流腮病毒的阳性率,差异具有统计学意义(χ^(2)=518.09,P<0.001)。结论上海市健康人群麻疹病毒IgG抗体水平较高,风疹和流腮病毒IgG抗体水平略低。

关键词：麻疹风疹流行性腮腺炎抗体监测

来源：评论

学校读者我要写书评

暂无评论

山东省不同酿酒葡萄种植园土壤质量现状分析

引用

山东农业科学 2021年第7期53卷 82-89页

作者：任艳华王超萍房经贵卢素文胡文效南京农业大学园艺学院江苏南京210018 山东省葡萄研究院山东济南250100 齐鲁工业大学生物工程学院山东济南250353

以山东省12个酿酒葡萄种植园土壤为研究对象,对酿酒葡萄种植园0~60cm土层的10个土壤理化指标进行调查分析,并采用综合指数评价法对种植园土壤质量进行综合性评价。结果表明:不同酿酒葡萄种植园土壤的理化指标差异显著,且随着土层深度的... 详细信息

以山东省12个酿酒葡萄种植园土壤为研究对象,对酿酒葡萄种植园0~60cm土层的10个土壤理化指标进行调查分析,并采用综合指数评价法对种植园土壤质量进行综合性评价。结果表明:不同酿酒葡萄种植园土壤的理化指标差异显著,且随着土层深度的增加,有机质含量和主要养分含量呈下降趋势。参照第二次全国土壤普查推荐的土壤肥力分级标准分级,不同酿酒葡萄种植园土壤在有机质方面,2、3、4级分别占16.70%、25.00%和58.33%;在全氮含量方面,8.33%、33.33%、33.33%和25.00%的样本分属于3、4、5、6级;在碱解氮方面,8.33%、25.00%、50.00%和16.67%的样本分别属于2、3、4、5级;在有效磷含量方面,16.67%、50.000%、16.67%和16.67%的样本分属2、3、4、5级;在速效钾含量方面,25.00%、33.33%、33.33%和8.33%的样本分属于2、3、4、5级。综合评价得出烟台种植园评分最高,土壤综合质量最好。针对上述酿酒葡萄种植园土壤的养分含量特征,建议对于不同种植园有针对性地制定土壤管理方案和改良措施,提升土壤质量。

关键词：酿酒葡萄种植园理化性质土壤质量综合指数评价法

来源：评论

学校读者我要写书评

暂无评论

Allosterically inhibited PFKL via prostaglandin E2 withholds glucose metabolism and ovarian cancer invasiveness

引用

CELL REPORTS 2023年第10期42卷 113246页

作者： Chen, Shengmiao Wu, Yiran Gao, Yang Wu, Chenxu Wang, Yuetong Hou, Chun ren, Miao Zhang, Shuyuan Zhu, Qi Zhang, Jiali Yao, Yufeng Huang, Mei Qi, Yingchuan B. Liu, Xue-Song Horng, Tiffany Wang, Haopeng Ye, Dan Zhu, Zhengjiang Zhao, suwen Fan, Gaofeng ShanghaiTech Univ Sch Life Sci & Technol Shanghai Peoples R China ShanghaiTech Univ iHuman Inst Shanghai Peoples R China Chinese Acad Sci Interdisciplinary Res Ctr Biol & Chem Shanghai Peoples R China Chinese Acad Sci Shanghai Inst Organ Chem Shanghai Peoples R China Fudan Univ Huadong Hosp Shanghai Key Lab Clin Geriatr Med Shanghai Peoples R China Minist Educ Key Lab Metab & Mol Med Shanghai Peoples R China Minist Sci & Technol Shanghai Key Lab Med Epigenet Int Colab Med Epigenet & Metab Shanghai Peoples R China Fudan Univ Inst Biomed Sci Shanghai Med Coll Mol & Cell Biol Lab Shanghai Peoples R China Fudan Univ Sch Life Sci Shanghai Peoples R China

Metastasis is the leading cause of high ovarian-cancer-related mortality worldwide. Three major processes constitute the whole metastatic cascade: invasion, intravasation, and extravasation. Tumor cells often reprogram their metabolism to gain advantages in proliferation and survival. However, whether and how those metabolic alterations contribute to the invasiveness of tumor cells has yet to be fully understood. Here we performed a genome-wide CRISPR-Cas9 screening to identify genes participating in tumor cell dissemination and revealed that PTGES3 acts as an invasion suppressor in ovarian cancer. Mechanistically, PTGES3 binds to phosphofructokinase, liver type (PFKL) and generates a local source of prostaglandin E2 (PGE2) to allosterically inhibit the enzymatic activity of PFKL. Repressed PFKL leads to downgraded glycolysis and the subsequent TCA cycle for glucose metabolism. However, ovarian cancer suppresses the expression of PTGES3 and disrupts the PTGES3-PGE2-PFKL inhibitory axis, leading to hyperactivation of glucose oxidation, eventually facilitating ovarian cancer cell motility and invasiveness.

关键词： CRISPR-Cas9 screening ovarian cancer invasion PTGES3 PFKL PGE2 EMT metastasis TET2 fumarate Research topic(s)CP: Cancer CP: Metabolism

来源：评论

学校读者我要写书评

暂无评论

磁敏感加权成像对出血性脑梗死再灌注出血的临床价值及影像分析

引用

中国当代医药 2022年第8期29卷 130-132,136页

作者：陈素纹龙光峰伍卓彬任泳基广东省江门市人民医院放射科广东江门529000

目的研究对出血性脑梗死患者再灌注出血情况采用磁敏感加权成像(SWI)检查的临床应用价值并对其检查结果进行影像学分析。方法选取2020年1月至2021年3月江门市人民医院放射科收治的出血性脑梗死患者70例作为研究对象。所有患者均采用1.5 ... 详细信息

目的研究对出血性脑梗死患者再灌注出血情况采用磁敏感加权成像(SWI)检查的临床应用价值并对其检查结果进行影像学分析。方法选取2020年1月至2021年3月江门市人民医院放射科收治的出血性脑梗死患者70例作为研究对象。所有患者均采用1.5 T超导MR成像仪进行检查,依次进行常规MRI平扫以及磁敏感加权成像检查。以数字减影血管造影技术(DSA)作为诊断金标准,比较不同MRI技术诊断效能、出血体积,并分析两种技术影像学特征。结果磁敏感加权成像检查、常规MRI平扫敏感度比较,差异无统计学意义(P>0.05)。磁敏感加权成像检查出血脑梗死再灌注出血准确度、特异度高于常规MRI平扫准确性,差异有统计学意义(P<0.05)。磁敏感加权成像检查出血体积大于常规MRI平扫出血体积均值,差异有统计学意义(P<0.05)。结论磁敏感加权成像检查对出血性脑梗死患者再灌注出血的准确性、特异度较高,可以尽可能完整扫描脑内出血范围,为临床治疗提供更加可靠的诊断结果,临床值得推广。

关键词：磁敏感加权成像出血性脑梗死再灌注出血临床价值影像学分析

来源：评论

学校读者我要写书评

暂无评论

Insights into the mechanism of cyanobacteria removal by the algicidal fungiBjerkandera adustaandTrametes versicolor

引用

MICROBIOLOGYOPEN 2020年第8期9卷 e1042页

作者： Han, Guomin Ma, Hui ren, Shenrong Gao, Xueyan He, Xiaolong Zhu, suwen Deng, Ruining Zhang, Shihua Anhui Agr Univ Sch Life Sci Hefei 230036 Peoples R China Anhui Agr Univ Natl Engn Lab Crop Stress Resistance Breeding Hefei Peoples R China Anhui Acad Agr Sci Rice Res Inst Key Lab Rice Genet Breeding Anhui Prov Hefei Peoples R China Anhui Agr Univ Inst Appl Math Hefei 230036 Peoples R China

Fungal mycelia can eliminate almost all cocultured cyanobacterial cells within a short time. However, molecular mechanisms of algicidal fungi are poorly understood. In this study, a time-course transcriptomic analysis of algicidal fungusBjerkandera adustaT1 was applied to investigate gene expression and regulation. A total of 132, 300, 422, and 823 differentially expressed genes (DEGs) were identified at 6, 12, 24, and 48 hr, respectively. Most DEGs exhibited high endopeptidase activity, cellulose catabolic process, and transmembrane transporter activity by using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Many decomposition genes encoding endopeptidases were induced a little later inB. adustaT1 when compared with previously investigated algicidal fungusTrametes versicolorF21a. Besides, the accumulated expression of Polysaccharide lyases8 (PL8) gene with peptidoglycan and alginate decomposition abilities was greatly delayed inB. adustaT1 relative toT. versicolorF21a. It was implied that endopeptidases and enzymes of PL8 might be responsible for the strong algicidal ability ofB. adustaT1 as well asT. versicolorF21a.

关键词： Algicidal fungi Algicidal mechanism Decomposition Endopeptidase Polysaccharide lyases8 Transcriptomic analysis

来源：评论

学校读者我要写书评

暂无评论

蒸汽发生器管束支撑结构对传热管完整性的影响分析

引用

压力容器 2020年第10期37卷 52-56页

作者：崔素文杨芝栋任红兵深圳中广核工程设计有限公司广东深圳518172

蒸汽发生器管束支撑结构对传热管完整性影响重大。以梅花孔支撑板和栅格支撑板这两种典型的管束支撑结构为例,分别对管束支撑结构的通透性、流场特性、间隙换热特性、泥渣沉积行为及其对流致振动性能的影响进行研究,以分析其对传热管完... 详细信息

蒸汽发生器管束支撑结构对传热管完整性影响重大。以梅花孔支撑板和栅格支撑板这两种典型的管束支撑结构为例,分别对管束支撑结构的通透性、流场特性、间隙换热特性、泥渣沉积行为及其对流致振动性能的影响进行研究,以分析其对传热管完整性的影响。根据分析结果,针对不同管束支撑结构的改进方向提出了建议。

关键词：蒸汽发生器传热管梅花孔支撑板栅格支撑板热工水力性能流致振动

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：