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Novel expression profiles of microRNAs suggest that specific miRNAs regulate gene expression for the sexual maturation of female Schistosoma japonicum after pairing

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PARASITES & VECTORS 2014年第1 p177-177期7卷 1-15页

作者： Sun, Jun Wang, suwen Li, Chen ren, Yijiu Wang, Jinqiang Tongji Univ Sch Med Inst Infect Dis & Vaccine Dev Shanghai 200092 Peoples R China

Background: Schistosoma japonicum is one of the major causative agents of schistosomiasis. The pairing of males and females leads to female sexual maturation and maintains this mature state. However, the mechanisms by which pairing facilitates sexual maturation are yet to be investigated. Methods: Parasites isolated from single-and double-sex cercariae-infected mice were analyzed by Solexa to uncover pair-regulated miRNA profiles. To reveal the biological functions of differentially expressed miRNAs among the samples, we predicted the target genes of these differentially expressed miRNAs and compared the gene expression between 23-d-old female schistosomula from double-sex infections (23DSI) and 23-d-old female schistosomula from single-sex infections (23SSI) by analyzing digital gene expression profiling (DGE). KEGG pathway analysis was used to investigate the relevant biological processes of these target genes to understand the significance of differentially expressed miRNAs after pairing. Results: The differentially expressed miRNA profiles of female 18-and 23-d post-single-and double-sex infections were analysed by Solexa. Similar miRNA profiles were observed in 18SSI and 18DSI, with the presence of identically expressed high-abundance miRNA, such as miRNA-1, miRNA-71b-5p and let-7. By contrast, in 23DSI and 23SSI, most of these high-abundance miRNAs were down-regulated. Furthermore, among all samples, bantam was distinctly up-regulated in 23 DSI, and miR-1, miR-71, miR-7-5p, and miR-7 were distinctly up-regulated in 23SSI. The transcriptomes of 23DSI and 23SSI revealed that the predicted target genes of miRNA-1, miRNA-71, miRNA-7, and miR-7-5p were associated with the ribonucleoprotein complex assembly and microtubule-based process. Conversely, the predicted target genes of bantam were related to the embryo development, development of primary sexual characteristics and regulation of transcription. KEGG pathway analysis revealed that in unpaired females, the hig

关键词： Schistosoma japonicum Pairing Solexa miRNA bantam miRNA-1 miRNA-71 miRNA-7 miR-7-5p

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Continuous pearl-necklace-shaped In2O3 ceramic nanofibers: Preparation, characterization and gas sensing properties

Continuous pearl-necklace-shaped In2O3 ceramic nanofibers: P...

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作者： Lu, Qifang Wang, Cuiqing Liu, suwen ren, Manman Key Lab. of Proc. and Test. Technol. of Glass and Funct. Ceramics of Shandong Province Shandong Institute of Light Industry Jinan 250353 China Lunan Research Institute of Coal Chemistry Jining 272000 China

Continuous pearl-necklace-shaped In2O3 ceramic nanofibers with diameters around 30∼100nm have been fabricated by electrospinning combined with annealing process used indium nitrate and poly(vinylpyrrolidone) (PVP) as raw materials. The specific surface area of In2O3 ceramic nanofibers comes up to 100m2/g. The morphology and chemical structure of the as-prepared and calcined samples were analyzed by scanning electron microscopy (SEM), thermal gravimetric analysis (TGA), high-resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques. The gas sensor fabricated by In2O3 ceramic nanofibers sintered at 700°C has special gas sensing properties including high response and selectivity, fast response and recovery time to ethanol gas. © 2011 The Japan Institute of Metals.

关键词： Thermogravimetric analysis

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Preparation and photoluminescence of Dy³⁺-doped Sr₅(PO₄)₃Cl nanorods

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JOURNAL OF ALLOYS AND COMPOUNDS 2006年第1-2期425卷 261-263页

作者： Xiu, Zhiliang Liu, suwen ren, Ming Liu, Jian Pan, Jie Cui, Xiaopeng Light Ind Shandong Coll Dept Mat Sci & Engn Jinan 250100 Peoples R China Shandong Pharmaceut Glass Co Ltd Zibo 256100 Peoples R China

Dy3+-doped Srs(PO4)(3)Cl nanocrystals were prepared by a precipitation method. Transmission electron microscopy (TEM) image showed that the products were mainly rod-like structures with diameters of similar to 50 nm and lengths of 300-500 nm. The photoluminescence properties of Dy3+ in Sr-5(PO4)(3)Cl host and luminescence quenching were discussed. (c) 2006 Elsevier B.V. All rights reserved.

关键词： nanorods precipitation photoluminescence

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Improved luminescence properties of europium complexes encapsulated into mesoporous SBA-15

Improved luminescence properties of europium complexes encap...

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作者： Li, suwen Song, Hongwei Li, Wenlian Lu, Shaozhe ren, Xinguang Key Laboratory of Excited State Physics Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences 16 Eastern Nan-Hu Road Changchun 130033 China Graduate School Chinese Academy of Sciences Beijing 100039 China

Two kinds of Eu3+ complexes-Eu(TTA)3(TPPO) 2 (TTA: thenoyltrifluoroacetone, TPPO: triphenylphosphineoxide) and Eu(BA)3(TPPO)2 (BA: 1-benzoylacetone) - were fully encapsulated, uniformly distributed into the channels of unmodified and modified SBA-15, and structurally characterized. The luminescent properties of the encapsulated complexes were systematically studied in contrast to the pure complexes. The results indicate that the excitation bands assigned to the π-π* electron transition of the ligands for Eu3+ complexes encapsulated in SBA-15 were split into different components, and the 5D0-7F0 transitions became partly allowed. The emission lines for the 5D0-7F 2 transitions became broader and the relative intensity for different crystal field components varied greatly in comparison to the pure complex. Most importantly, the photostability and thermostability of the emissions improved considerably. Copyright © 2008 American Scientific Publishers All rights reserved.

关键词： Europium compounds

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Synthesis and optical properties of Pb-doped Srs(PO₄)₃Cl nanorods

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JOURNAL OF ALLOYS AND COMPOUNDS 2007年第1-2期441卷 219-221页

作者： Xiu, Zhiliang Liu, suwen Xu, Fengxiu ren, Min Pan, Tie Cui, Xiaopeng Yu, Weina Yu, Jiaoxian Shandong Inst Light Ind Dept Mat Sci & Engn Jinan 250353 Peoples R China

Pb-doped Sr-5(PO4)(3)Cl nanocrystals with rod-like structures were synthesized by a simple precipitation method. The nanorods had diameters of similar to 50 nm and lengths of 150-350 nm observed by transmission electron micrograph (TEM). The photoluminescence spectra revealed that a strong red emission at 725 nm was ascribed to Pb-related charge-transfer transition. The luminescence intensities were strongly dependent on the concentration of Pb 21 ions. (C) 2006 Published by Elsevier B.V.

关键词： nanorods precipitation photoluminescence

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Novel energy-transfer route and enhanced luminescent properties in YVO4:Eu³⁺/YBO₃:Eu³⁺ composite

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CHEMISTRY OF MATERIALS 2006年第18期18卷 4526-4532页

作者： Pan, Guohui Song, Hongwei Bai, Xue Liu, Zhongxin Yu, Hongquan Di, Weihua Li, suwen Fan, Libo ren, Xinguang Lu, Shaozhe Chinese Acad Sci Changchun Inst Opt Fine Mech & Phys Key Lab Excited State Phys Changchun 130033 Peoples R China Chinese Acad Sci Grad Sch Beijing 100039 Peoples R China

In this paper, YVO4: Eu3+/YBO3:Eu3+ composite was fabricated by chemical corrosion through a two-step hydrothermal process, i.e., first preparation of YBO3:Eu3+ nanocrystals and subsequent chemical corrosion by Na3VO4 solution. A novel energy-transfer and luminescent route via UV and VUV excitation was identified (YBO3 phase -> YVO4 phase -> Eu3+ ions in the YVO4 phase), which remained in the sample annealed at high temperature. In this composite high quantum yield was maintained, and considerably improved color purity was induced. A corrosive interface between the YVO4: Eu3+ and YBO3: Eu3+ phases was identified by the site-selective excitation technique, where the local environment surrounding Eu3+ was extremely complex. It is believed that, through further optimization, this novel composite can be candidate phosphors for red PDPs and Hg-free fluorescent lamp in the future.

关键词： Composites Energy transfer Ions Physical and chemical processes Nanocrystals

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Polyol-mediated syntheses and characterizations of NaYF₄, NH₄Y₃F₁₀ and YF₃ nanocrystals/sub-microcrystals

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MATERIALS RESEARCH BULLETIN 2008年第8-9期43卷 2130-2136页

作者： Qin, Ruifei Song, Hongwei Pan, Guohui Hu, Lanying Yu, Hongquan Li, suwen Bai, Xue Fan, Libo Dai, Qilin ren, Xinguang Zhao, Haifeng Wang, Tie Chinese Acad Sci Changchun Inst Opt Fine Mech & Phys Key Lab Excited State Phys Changchun Peoples R China Chinese Acad Sci Grad Sch Beijing 100039 Peoples R China

In this paper, NaYF4 nanocrystals and NH4Y3F10 sub-microcrystals were prepared using a polyol method. After being annealed, NH4Y3F10 can convert into YF3, which is a very efficient host matrix for upconversion. The structures of obtained NaYF4, NH4Y3F10 and YF3 samples are pure cubic phase, a mixture of cubic and hexagonal phase and pure orthorhombic phase, respectively. The size of obtained NaYF4 nanocrystals is about 20 nm, and those of NH4Y3F10 and YF3 sub-microcrystals are both about 200 nm. When co-doped with Er3+ and Yb3+, NaYF4 and YF3 samples can emit bright light under 978 nm excitation. Upconversion mechanisms in Yb3+/Er3+ co-doped NaYF4 and YF3 samples were discussed. (c) 2007 Elsevier Ltd. All rights reserved.

关键词： fluorides nanostructures luminescence

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Highly luminescent YVO₄-Eu³⁺ nanocrystals coating on wirelike Y(OH)₃-Eu³⁺ and Y₂O₃-Eu³⁺ microcrystals by chemical corrosion

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JOURNAL OF PHYSICAL CHEMISTRY C 2007年第33期111卷 12472-12477页

作者： Pan, Guohui Song, Hongwei Bai, Xue Fan, Libo Yu, Hongquan Dai, Qilin Dong, Biao Qin, Ruifei Li, suwen Lu, Shaozhe ren, Xinguang Zhao, Haifeng Chinese Acad Sci Changchun Inst Opt Fine Mech & Phys Key Lab Excited State Phys Changchun 130033 Peoples R China Chinese Acad Sci Grad Sch Beijing 100039 Peoples R China

Highly luminescent YVO4-Eu3+ nanocrystals (NCs) coating on wirelike Y(OH)(3)-Eu3+ and Y2O3-Eu3+ microcrystals were synthesized by a chemical corrosion method, that is, first preparation of Y(OH)(3)-Eu3+ wirelike microcrystals by a hydrothermal method, and subsequently chemical corrosion with NaVO4 solutions through a second hydrothermal treatment. The crystal structure, morphology, and optical properties of the composites were characterized and compared with those of the general nanoparticles (NPs) obtained by a one-step hydrothermal process. The results indicate that chemical corrosion method yields high-quality YVO4-Eu3+ NCs in the composite grown from exterior to interior, compared with the directly hydrothermal NPs grown from interior to exterior. In addition, luminescent intensity for YVO4-Eu3+ in the composite was remarkably increased compared with that of NPs, due to more efficient energy transfer from VO43- to Eu3+ ions, and was further increased up to level of the the bulk materials after being annealed at 500 degrees C for 2 h. The local environment surrounding Eu3+ in the composite is more complex due to the presence of corrosive interface.

关键词： Electrochemistry Luminescence Composites Ions Oxidation

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Improved photoluminescence properties of ternary terbium complexes in mesoporous molecule sieves

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JOURNAL OF PHYSICAL CHEMISTRY B 2006年第46期110卷 23164-23169页

作者： Li, suwen Song, Hongwei Li, Wenlian ren, Xinguang Lu, Shaozhe Pan, Guohui Fan, Libo Yu, Hongquan Zhang, Hui Qin, Ruifei Dai, QiLin Wang, Tie Chinese Acad Sci Changchun Inst Opt Fine Mech & Phys Key Lab Excited State Phys Changchun 130033 Peoples R China Chinese Acad Sci Grad Sch Changchun 130033 Peoples R China

Ternary terbium complexes were fully encapsulated and uniformly distributed into the channels of unmodified and modified mesoporous molecule sieves of SBA-15 and characterized by transmission electron micrographs (TEM), Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible (UV-vis) absorption spectra, inductively coupled plasma-atomic emission spectroscopy (ICP-AES), and elemental analysis. The luminescent properties for the encapsulated complexes were systematically studied in contrast to the pure complexes, including excitation and emission spectra, fluorescence dynamics, photostability under UV exposure, and the temperature dependence of intensity and lifetime. The results indicate that the excitation bands assigned to the pi-pi* electron transition of the ligands for Tb complexes encapsulated in SBA- 15 were split into different components due to decreased symmetry and disappeared at long wavelengths. Owing to suppressed vibration transitions, the outer quantum efficiency of the D-5(4)-F-7(J) (J=0-5) emissions was enhanced largely in comparison to the pure complexes. In addition, the photostability and thermostability of the emissions were also improved considerably.

关键词： TERBIUM PHOTOLUMINESCENCE TRANSMISSION electron microscopy FOURIER transform infrared spectroscopy

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Electrospinning preparation and photoluminescence properties of rare-earth complex/polymer composite fibers

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JOURNAL OF PHYSICAL CHEMISTRY C 2007年第17期111卷 6524-6527页

作者： Zhang, Hui Song, Hongwei Yu, Hongquan Bai, Xue Li, suwen Pan, Guohui Dai, Qilin Wang, Tie Li, Wenlian Lu, Shaozhe ren, Xinguang Zhao, Haifeng Chinese Acad Sci Changchun Inst Opt Fine Mech & Phys Key Lab Excited State Phys Changchun 130033 Peoples R China Chinese Acad Sci Grad Sch Beijing 10039 Peoples R China

Composite fibers of poly(vinyl pyrrolidone) (PVP, M-w approximate to 1 300 000) and europium complex Eu(BA)(3)(TPPO)(2) (BA =1-benzoylacetone;TPPO = triphenylphosphine oxide) with strong photoluminescence were prepared by electrospinning. Their luminescent properties were studied in comparison to that of the pure complex. The results indicate that due to decreased symmetry in the composites the excitation bands are split into two different components, peaking at similar to 246 and similar to 336 nm, respectively, while the ratio of the electronic dipole D-5(0)-F-7(2) transitions to the magnetic dipole D-5(0)-F-7(1) ones in the emission spectra becomes a little larger. The room temperature fluorescence lifetime for the D-5(0) level becomes shorter due to increased radiative and nonradiative transition rates. The temperature dependence of photoluminescence was investigated under 325-nm excitation. It is interesting to observe that in the composite fiber the total emission intensity of the Eu3+ ions changes little below 150 K, while in the pure complex the intensity decreases monotonically with increasing temperature. In addition, the composite fibers exhibit better photostability exposed to ultraviolet lights. These novel luminescent composite fibers have potential application in nanodevices.

关键词： Photoluminescence Fibers Composites Fluorescence Ions

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