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Physical activity levels and influencing factors among pregnant women in China: A systematic review and meta-analysis

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INTERNATIONAL JOURNAL OF NURSING STUDIES 2024年 158卷 104841页

作者： Zhang, Wei Zhang, Le Xu, Ping Guo, Pingping Mao, Minna zhao, Rujia Feng, suwen Zhejiang Univ Womens Hosp Sch Med 1 Xue Shi Rd Hangzhou 310006 Zhejiang Peoples R China Zhejiang Univ Sch Med 866 Yu Hang Tang Rd Hangzhou 310058 Zhejiang Peoples R China

Background: Physical activity during pregnancy has been recommended as an effective measure to prevent various pregnancy complications. However, global physical activity participation during pregnancy is not optimal, and the factors influencing physical activity participation during pregnancy need to be further investigated. In China, where there are no localized guidelines for physical activity during pregnancy, the prevalence of meeting recommended physical activity levels among Chinese pregnant women is still unclear. Objectives: This study aimed to comprehensively review the prevalence of meeting recommended physical activity levels among pregnant women in China and to further explore the factors influencing physical activity participation during pregnancy. Design: This was a systematic review and meta-analysis. Methods: A systematic review was conducted in both English and Chinese databases from inception until August 1, 2023. Two reviewers independently screened literature, assessed study eligibility and extracted data. The Agency for Healthcare Research and Quality was used to evaluate methodological quality of included studies. The pooled prevalence was calculated using a random-effects model. Subgroup analyses were conducted to explore sources of heterogeneity. Results: A total of 12 cross-sectional studies, including 11,323 Chinese pregnant women with a mean age ranging from 27.0 to 33.0 years, met the inclusion criteria. The prevalence of meeting recommended physical activity levels among pregnant women in China was 21.0 % (95 % confidence interval (CI):12.5 %-29.5 %). A higher prevalence of meeting recommended physical activity levels was observed among pregnant women who were in their second trimester, who were living in the southern region, who were assessed by validated questionnaires, and who used 150 min physical activity per week as a criterion for meeting recommendation. Conclusions: The findings suggested a low prevalence of meeting recommended p

关键词： Physical activity Pregnant women China Prevalence Meta-analysis

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Peridynamic modeling of stochastic fractures in bolted glass plates

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MECHANICS RESEARCH COMMUNICATIONS 2022年 122卷

作者： Zhu, Fan zhao, Jidong Ballarini, Roberto Peng, Shitao Chen, suwen Hong Kong Univ Sci & Technol Dept Civil & Environm Engn Clearwater Bay Hksar Peoples R China Univ Houston Dept Civil & Environm Engn Cullen Coll Engn Houston TX 77204 USA Tongji Univ Coll Civil Engn Shanghai Peoples R China Tongji Univ State Key Lab Disaster Reduct Civil Engn Shanghai Peoples R China

This paper presents a peridynamics-based computational approach for modeling fractures in bolted glass plates that features an explicit consideration of randomly distributed micro-flaws. The glass fabrication procedures are assumed to produce a stochastic distribution of a single population of Pareto-distributed surface flaws and mono-sized edge flaws. Numerical simulations are presented of the cracking that develops for varying geometrical configurations of the glass plates under in-plane loads, using a plane stress ordinary state-based peridynamics formulation. The fracture patterns and failure loads predicted by the models agree reasonably well with corre-sponding experimental observations. The results from a series of simulations suggest that the distribution of strength can be fitted by the Weibull distribution or normal distribution, but is strongly dependent on the assumed values of edge flaw density and flaw depth.

关键词： Peridynamics Glass fracture Bolt connection Flaw Strength statistics

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Molecular Basis for the Final Oxidative Rearrangement Steps in Chartreusin Biosynthesis

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JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2018年第34期140卷 10909-10914页

作者： Wang, Yi Shuang Zhang, Bo Zhu, Jiapeng Yang, Cheng Long Guo, Yu Liu, Cheng Li Liu, Fang Huang, Huiqin zhao, suwen Liang, Yong Jiao, Rui Hua Tan, Ren Xiang Ge, Hui Ming Nanjing Univ Inst Funct Biomol Sch Life Sci State Key Lab Pharmaceut Biotechnol Nanjing 210023 Jiangsu Peoples R China Nanjing Univ Chinese Med Sch Med & Life Sci State Key Lab Cultivat Base TCM Qual & Efficacy Nanjing 210023 Jiangsu Peoples R China Shanghai Tech Univ iHuman Inst Shanghai 201210 Peoples R China Chinese Acad Trop Agr Sci Minist Agr Key Lab Biol & Genet Resources Trop Crops Inst Trop Biosci & Biotechnol Haikou 571101 Hainan Peoples R China Nanjing Univ Sch Chem & Chem Engn Jiangsu Key Lab Adv Organ Mat State Key Lab Coordinat Chem Nanjing 210023 Jiangsu Peoples R China

Oxidative rearrangements play key roles in introducing structural complexity and biological activities of natural products biosynthesized by type II polyketide synthases (PKSs). Chartreusin (1) is a potent antitumor polyketide that contains a unique rearranged pentacyclic aromatic bilactone aglycone derived from a type II PKS. Herein, we report an unprecedented dioxygenase, ChaP, that catalyzes the final a-pyrone ring formation in 1 biosynthesis using flavin-activated oxygen as an oxidant. The X-ray crystal structures of ChaP and two homologues, docking studies, and site-directed mutagenesis provided insights into the molecular basis of the oxidative rearrangement that involves two successive C-C bond cleavage steps followed by lactonization. ChaP is the first example of a dioxygenase that requires a flavin-activated oxygen as a substrate despite lacking flavin binding sites, and represents a new class in the vicinal oxygen chelate enzyme superfamily.

关键词： Peptides and proteins Monomers Biosynthesis Aromatic compounds Rearrangement

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Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein

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PLACENTA 2022年 130卷 46-52页

作者： Yu, Yan Peng, Qiaohua zhao, Peng Wang, Lingfang Weng, Yang Chen, Xiaojing Li, Xingxia Feng, suwen Wang, Xinyu Lu, Weiguo Xie, Xing Cheng, Xiaodong Zhejiang Univ Womens Hosp Sch Med Dept Gynaecol Oncol Hangzhou Zhejiang Peoples R China Zhejiang Univ Womens Hosp Sch Med Key Lab Womens Reprod Hlth Zhejiang Prov Hangzhou Zhejiang Peoples R China

Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in ***: Choriocarcinoma cell lines, JAR and JEG-3, were transfected with lentivirus carrying the MIIP-inter-fering RNA (to downregulate MIIP expression) or left untransfected (negative control). Cell migration and in-vasion were studied using transwell migration assays and scratch assays. In vivo tumour burden was studied using tumour xenograft models in specific-pathogen-free nude mice and live imaging. We elucidated possible molecular signalling pathways using western ***: In transwell migration and scratch assays MIIP-downregulated JAR and JEG-3 cells migrated and invaded faster compared to their respective negative control cells. Migration and invasion by the MIIP-upregulated SWAN cells was slower than that by negative control SWAN cells. Live imaging revealed that bioluminescence values were higher in MIIP-downregulated tumours than in the negative control tumours. Mice with MIIP-down-regulated tumours had higher serum human chorionic gonadotropin (HCG) levels than those with negative control tumours. The MIIP expression was negatively correlated with that of histone deacetylase (HDAC6) and positively correlated with that of acetylated ***: Thus, MIIP-by inhibiting cellular motility in choriocarcinoma-acts as a tumour suppressor gene. This highlights a potential therapeutic target for refractory choriocarcinoma. Additionally, HDAC6 and acety-lated alpha-tubulin may be involved in the regulatory effects of MIIP on the biobehaviour of choriocarcinoma cells.

关键词： Choriocarcinoma Migration and invasion inhibitory protein MIIP Invasion Metastasis

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ZMYND8 Reads H3K36me2 to Activate CEBPE Transcription and Suppress Multiple Myeloma Progression through the Inhibition of Adaptive UPR Pathways

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ADVANCED SCIENCE 2025年第20期12卷 e2409219页

作者： Xu, Jiaxuan Dong, Xiaoqing Peng, Yue Yan, Jie Xu, Peipei Li, Feiyu Zhang, suwen Chen, Lanxin Meng, Xingjun Wang, Kangning Xing, Mengying Li, Wenyang Huang, David C. S. zhao, Quan Chen, Bing Nanjing Univ Nanjing Drum Tower Hosp Clin Coll Nanjing Univ Chi China Australia Inst Translat Med Sch Life Sci Affiliated HospMed SchDept HematolState Key Lab Nanjing 210023 Peoples R China Nanjing Univ Chinese Med Sch Hlth Preservat & Rehabil Nanjing 210023 Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Inst Basic Med Sci Beijing 100005 Peoples R China Univ Melbourne Walter & Eliza Hall Inst Med Res Dept Med Biol Melbourne Vic 3052 Australia

Multiple myeloma (MM) pathogenesis is closely associated with aberrant epigenetic regulation and resulting modifications, such as dimethylation of lysine 36 in histone H3 (H3K36me2). However, the recognition signature of H3K36me2 and its functional role in MM remain largely unknown. Here, the zinc-finger MYND-type-containing 8 (ZMYND8) is identified as a potential reader of the H3K36me2 mark that suppresses MM progression. ZMYND8 knockdown promotes the proliferation and invasion of MM cells. Combined transcriptomic and epigenomic analyses reveal that CCAAT/enhancer-binding protein epsilon (CEBPE) is a direct downstream target of ZMYND8. CEBPE modulates adaptive unfolded protein response (UPR) pathways through the transcriptional repression of ERN1, XBP1, and ATF6 to impair cell survival. Coimmunoprecipitation and chromatin immunoprecipitation assays show that ZMYND8 activates CEBPE expression in an H3K36me2-dependent manner and that its Pro-Trp-Trp-Pro domain is required for binding H3K36me2 modules, leading to CEBPE transcription. Low ZMYND8 expression is significantly correlated with adverse clinicopathological features and poor survival outcomes in MM patients. Furthermore, ZMYND8 upregulation increases the sensitivity of MM cells to carfilzomib. Taken together, these findings demonstrate that ZMYND8 epigenetically activates CEBPE transcription and suppresses MM cell growth by inhibiting the adaptive UPR, suggesting that ZMYND8 can be a novel therapeutic target for patients with MM.

关键词： carfilzomib CEBPE multiple myeloma ZMYND8

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Human Female Reproductive System Organoids: Applications in Developmental Biology, Disease Modelling, and Drug Discovery

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STEM CELL REVIEWS AND REPORTS 2020年第6期16卷 1173-1184页

作者： Cui, Yutong zhao, Huanqiang Wu, suwen Li, Xiaotian Fudan Univ Obstet & Gynaecol Hosp Shanghai Peoples R China Fudan Univ Shanghai Key Lab Female Reprod Endocrine Related Shanghai Peoples R China Fudan Univ Inst Biomed Sci Shanghai Peoples R China

Organoid technique has achieved significant progress in recent years, owing to the rapid development of the three-dimensional (3D) culture techniques in adult stem cells (ASCs) and pluripotent stem cells (PSCs) that are capable of self-renewal and induced differentiation. However, our understanding of human female reproductive system organoids is in its infancy. Recently, scientists have established self-organizing 3D organoids for human endometrium, fallopian tubes, oocyte, and trophoblasts by culturing stem cells with a cocktail of cytokines in a 3D scaffold. These organoids express multicellular biomarkers and show functional characteristics similar to those of their origin organs, which provide potential avenues to explore reproductive system development, disease modelling, and patient-specific therapy. Nevertheless, advanced culture methods, such as co-culture system, 3D bioprinting and organoid-on-a-chip technology, remain to be explored, and more efforts should be made for further elucidation of cell-cell crosstalk. This review describes the development and applications of human female reproductive system organoids.

关键词： Disease modelling 3D remodeling Endometrium Fallopian tube Follicle Organoids Stem cell Trophoblast

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Extra villous trophoblast-derived PDL1 can ameliorate macrophage inflammation and promote immune adaptation associated with preeclampsia

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JOURNAL OF REPRODUCTIVE IMMUNOLOGY 2024年 161卷 104186-104186页

作者： Cui, Yutong Wu, suwen Liu, Ketong zhao, Huanqiang Ma, Bo Gong, Lili Zhou, Qiongjie Li, Xiaotian Fudan Univ Obstet & Gynaecol Hosp Dept Obstet Shanghai Peoples R China Shenzhen Matern & Child Healthcare Hosp Dept Obstet Shenzhen Guangdong Peoples R China Fudan Univ Shanghai Key Lab Female Reprod Endocrine Related D Shanghai Peoples R China

Introduction: Severe preeclampsia (sPE) is a systemic syndrome that may originate from chronic inflammation. Maintaining maternal-fetal hemostasis by the co-inhibitory molecule programmed death ligand 1 (PDL1) can be favorable for ameliorating inflammation from immune cells. Apart from programmed death 1 (PD1) expression, decidual macrophages (dMs) produce inflammatory cytokines, in response to cells which express PDL1. However, strong evidence is lacking regarding whether the PDL1/PD1 interaction between trophoblasts and decidual macrophages affects inflammation during sPE development. Methods: To determine whether the trophoblast-macrophage crosstalk via the PDL1/PD1 axis modulates the inflammatory response in sPE-like conditions, at first, maternal-fetal tissues from sPE and normal patients were collected, and the PDL1/PD1 distribution was analyzed by Western blot, immunohistochemistry/ immunofluorescence and flow cytometry. Next, a coculture system was established and flow cytometry was used to identify how PDL1 was involved in macrophage-related inflammation under hypoxic stress. Transcriptional analysis was performed to clarify the inflammation-associated pathway induced by the PDL1/PD1 interaction. Finally, the Nwnitro-L-arginine methyl ester hydrochloride (L-NAME) mouse model was used to examine the effect of PDL1 on macrophage-related inflammation by measuring PE-like symptoms. Results: In maternal-fetal tissue from sPE patients, placental extravillous trophoblasts (EVTs) and dMs had a surprisingly increase of PDL1 and PD1 expression, respectively, accompanied by a higher percentage of CD68 +CD86 + dMs. In vitro experiments showed that trophoblast-derived PDL1 under hypoxia interacted with PD1 on CD14 +CD80 +macrophages, leading to suppression of inflammation through the TNFa-p38/NFxB pathway. Accordingly, the PE-like mouse model showed a reversal of PE-like symptoms and a reduced F4/80 + CD86 + macrophage percentage in the uterus in response to recombinant

关键词： Preeclampsia PDL1 Maternal-fetal interaction Inflammation Adaptation

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Single-cell RNA-seq highlights a specific carcinoembryonic cluster in ovarian cancer

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CELL DEATH & DISEASE 2021年第11期12卷 1-11页

作者： zhao, Hongyu Gao, Yan Miao, Jinwei Chen, suwen Li, Jie Li, Zhefeng Yin, Chenghong Yue, Wentao Capital Med Univ Beijing Obstet & Gynecol Hosp Cent Lab Beijing 100026 Peoples R China Capital Med Univ Beijing Obstet & Gynecol Hosp Dept Gynecol Oncol Beijing 100026 Peoples R China Capital Med Univ Beijing Obstet & Gynecol Hosp Dept Family Planning Beijing 100026 Peoples R China

Expounding the heterogeneity for ovarian cancer (OC) with the cognition in developmental biology might be helpful to search for robust prognostic markers and effective treatments. In the present study, we employed single-cell RNA-seq with ovarian cancers, normal ovary, and embryo tissue to explore their heterogeneity. Then the differentiation process of clusters was explored;the pivotal cluster and markers were identified. Furthermore, the consensus clustering algorithm was used to explore the different clinical phenotypes in OC. At last, a prognostic model was construct and used to assess the prognosis for OCs. As a result, eight diverse clusters were identified, and the similarity existed in some clusters between embryo and tumours based on their gene expression. Meaningfully, a subtype of malignant epithelial cluster, PEG10(+) EME, was associated with poor survival and was an intermediate stage of embryo to tumour. PEG10 was a CSC marker and might influence CSC self-renewal and promote cisplatin resistance via NOTCH pathway. Utilising specific gene profiles of PEG10(+) EME based on public data sets, four phenotypes with different survival and clinical response to anti-PD-1/PD-L1 immunotherapy were identified. These insights allowed for the investigation of single-cell transcriptome of OCs and embryo, which advanced our current understanding of OC pathogenesis and resulted in promising therapeutic strategies.

关键词： Cancer genomics Tumour immunology

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Molecular Mechanism for Ligand Recognition and Subtype Selectivity of α2C Adrenergic Receptor

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CELL REPORTS 2019年第10期29卷 2936-+页

作者： Chen, Xiaoyu Xu, Yueming Qu, Lu Wu, Lijie Han, Gye Won Guo, Yu Wu, Yiran Zhou, Qingtong Sun, Qianqian Chu, Cenfeng Yang, Jie Yang, Liu Wang, Quan Yuan, Shuguang Wang, Ling Hu, Tao Tao, Houchao Sun, Yaping Song, Yunpeng Hu, Liaoyuan Liu, Zhi-Jie Stevens, Raymond C. zhao, suwen Wu, Dong Zhong, Guisheng ShanghaiTech Univ iHuman Inst Shanghai 201210 Peoples R China ShanghaiTech Univ Sch Life Sci & Technol Shanghai 201210 Peoples R China Chinese Acad Sci Shanghai Inst Biochem & Cell Biol CAS Ctr Excellence Mol Cell Sci Shanghai Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Chinese Acad Sci Inst Biophys Natl Lab Biomacromol Beijing 100101 Peoples R China Univ Southern Calif Bridge Inst Dept Biol Sci Los Angeles CA 90089 USA Univ Southern Calif Bridge Inst Dept Chem Los Angeles CA 90089 USA Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Guangdong Peoples R China Univ Warsaw Fac Chem Lab Biomodelling PL-02093 Warsaw Poland Univ Warsaw Biol & Chem Res Ctr PL-02093 Warsaw Poland Amgen Biopharmaceut R&D Shanghai Amgen Asia R&D Ctr Shanghai 201210 Peoples R China

Adrenergic G-protein-coupled receptors (GPCRs) mediate different cellular signaling pathways in the presence of endogenous catecholamines and play important roles in both physiological and pathological conditions. Extensive studies have been carried out to investigate the structure and function of beta adrenergic receptors (beta ARs). However, the structure of alpha adrenergic receptors (alpha ARs) remains to be determined. Here, we report the structure of the human alpha(2C) adrenergic receptor (alpha(2C)AR) with the non-selective antagonist, RS79948, at 2.8 angstrom. Our structure, mutations, modeling, and functional experiments indicate that a alpha(2C)AR-specific D206(ECL2)-R409(ECL3)-Y405(6.58) network plays a role in determining alpha(2) adrenergic subtype selectivity. Furthermore, our results show that a specific loosened helix at the top of TM4 in alpha(2C)AR is involved in receptor activation. Together, our structure of human alpha(2C)AR-RS79948 provides key insight into the mechanism underlying the alpha(2) adrenergic receptor activation and subtype selectivity.

关键词： α2C adrenergic receptor subtype selectivity crystal structure GPCRs Raynaud’s syndrome JP1302

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Genome-wide CRISPR screen identifies FAM49B as a key regulator of actin dynamics and T cell activation

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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2018年第17期115卷 E4051-E4060页

作者： Shang, Wanjing Jiang, Yong Boettcher, Michael Ding, Kang Mollenauer, Marianne Liu, Zhongyi Wen, Xiaofeng Liu, Chang Hao, Piliang zhao, suwen McManus, Michael T. Wei, Lai Weiss, Arthur Wang, Haopeng ShanghaiTech Univ Sch Life Sci & Technol Shanghai 201210 Peoples R China Chinese Acad Sci Shanghai Inst Biol Sci Inst Biochem & Cell Biol Shanghai 200031 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Univ Calif San Francisco Dept Microbiol & Immunol WM Keck Ctr Noncoding RNAs Ctr Diabet San Francisco CA 94143 USA ShanghaiTech Univ iHuman Inst Shanghai 201210 Peoples R China Univ Calif San Francisco Dept Med Rosalind Russell & Ephraim P Engleman Rheumatol R Div Rheumatol San Francisco CA 94143 USA Univ Calif San Francisco Howard Hughes Med Inst San Francisco CA 94143 USA Sun Yat Sen Univ Zhongshan Ophthalm Ctr State Key Lab Ophthalmol Guangzhou 510060 Guangdong Peoples R China

Despite decades of research, mechanisms controlling T cell activation remain only partially understood, which hampers T cell-based immune cancer therapies. Here, we performed a genome-wide CRISPR screen to search for genes that regulate T cell activation. Our screen confirmed many of the known regulators in proximal T cell receptor signaling and, importantly, also uncovered a previously uncharacterized regulator, FAM49B (family with sequence similarity 49 member B). FAM49B deficiency led to hyperactivation of Jurkat T cells following T cell receptor stimulation, as indicated by enhancement of CD69 induction, PAK phosphorylation, and actin assembly. FAM49B directly interacted with the active form of the small GTPase Rac, and genetic disruption of the FAM49BRac interaction compromised FAM49B function. Thus, FAM49B inhibits T cell activation by repressing Rac activity and modulating cytoskeleton reorganization.

关键词： genome-wide CRISPR screen TCR signaling FAM49B Rac1 actin cytoskeleton

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