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A maize NAC transcription factor, ZmNAC34, negatively regulates starch synthesis in rice

PLANT CELL REPORTS 2019年第12期38卷 1473-1484页

作者： Peng, Xiaojian Wang, Qianqian Wang, Yu Cheng, Beijiu zhao, Yang Zhu, suwen Anhui Agr Univ Sch Life Sci Natl Engn Lab Crop Stress Resistance Breeding Hefei 230036 Anhui Peoples R China Anhui Acad Agr Sci Inst Hort Hefei 230016 Anhui Peoples R China

Key message ZmNAC34 might function as an important regulator of starch synthesis by decreasing total starch accumulation and soluble sugar content and increasing amylose fractions. Starch is a major component in endosperm and directly influences seed yield and the cooking quality of cereal grains. Starch is synthesized through a series of complex biological processes. Nevertheless, the mechanism by which starch biosynthesis is regulated in maize is still unclear. In this study, ZmNAC34, a NAC transcription factor related to starch synthesis, was screened based on transcriptome sequencing data. Subsequent qRT-PCR analysis showed that ZmNAC34 is specifically expressed in maize endosperm. Transactivation and subcellular localization assays revealed that ZmNAC34 possesses two characteristics of transcription factors: nuclear localization and transactivation activity. Overexpression of ZmNAC34 in rice decreased total starch accumulation and soluble sugar content, while increased amylose fractions. Meanwhile, the transgenic seeds exhibited alterant starch structure and abnormal morphology. In addition, compared with WT seeds, most of the 17 starch biosynthesis-related genes were significantly upregulated in transgenic seeds from 6 to 15 DAP (day after pollination). These data reveal that ZmNAC34 might function as an important regulator of starch synthesis, thus providing a new perspective on controlling seed yield and quality.

关键词： Maize ZmNAC34 Endosperm Starch synthesis

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Physical interaction of tropomyosin 3 and STIM1 regulates vascular smooth muscle contractility and contributes to hypertension

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BIOMEDICINE & PHARMACOTHERAPY 2021年 134卷 111126-111126页

作者： Xu, Fangfang Zhu, Jinhang Chen, Ye He, Ke Guo, Jizheng Bai, suwen zhao, Ren Du, Juan Shen, Bing Anhui Med Univ Sch Basic Med Sci 81 Mei Shan Rd Hefei 230032 Anhui Peoples R China Anhui Med Univ Affiliated Hosp 1 Dept Cardiol Hefei 230032 Anhui Peoples R China

Scope: Tropomyosin (TPM), an actin-binding protein widely expressed across different cell types, is primarily involved in cellular contractile processes. We investigated whether TPM3 physically and functionally interacts with stromal interaction molecule 1 (STIM1) to contribute to vascular smooth muscle cell (VSMC) contraction, store-operated calcium entry (SOCE), and high-salt intake-induced hypertension in rats. Methods and results: Analysis of a rat RNA-seq data set of 80 samples showed that the STIM1 and Tpm3 transcriptome expression pattern is highly correlated, and co-immunoprecipitation results indicated that TPM3 and STIM1 proteins physically interacted in rat VSMCs. Immunohistochemical data displayed obvious co-localization of TPM3 and STIM1 in rat VSMCs. Knockdown of TPM3 or STIM1 in VSMCs with specific small interfering RNA significantly suppressed contractions in tension measurement assays and decreased SOCE in calcium assays. Rats fed a high-salt diet for 4 weeks had significantly higher systolic blood pressure than controls, with significantly increased contractility and markedly increased TPM3 and STIM1 expression levels in the mesenteric resistance artery (shown by tension measurements and immunoblotting, respectively). Additionally, high salt environment in vitro induced significant enhancement of TPM3 and STIM1 expression levels in VSMCs. Conclusions: We showed for the first time that TPM3 and STIM1 physically and functionally interact to contribute to VSMC contraction, SOCE, and high-salt intake-induced hypertension. Our findings provide mechanistic insights and offer a potential therapeutic target for high-salt intake-induced hypertension.

关键词： TPM3 STIM1 High-salt intake Vascular smooth muscle cell Store-operated calcium entry Vasoconstriction

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深水水下井口头系统紧锁机构分析

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石油矿场机械 2018年第2期47卷 63-68页

作者：孙子刚关清涛赵苏文李健王养锋中海油能源发展股份有限公司工程技术深圳分公司广东深圳518067

锁紧机构作为连接高低压井口头的重要部件,决定了深水井口头系统在多种外载条件下工作的稳定性和可靠性,对深水油气田作业安全至关重要。介绍了国内外几种典型水下高低压井口头系统及锁紧机构类型,对比分析了不同类型水下高低压井口头... 详细信息

锁紧机构作为连接高低压井口头的重要部件,决定了深水井口头系统在多种外载条件下工作的稳定性和可靠性,对深水油气田作业安全至关重要。介绍了国内外几种典型水下高低压井口头系统及锁紧机构类型,对比分析了不同类型水下高低压井口头系统采用的锁紧机构及其锁紧原理和特点,并对Dril-Quip公司SS-15型井口头系统采用有限元进行了力学分析。结论表明,采取较大预紧力的锁紧机构,可以有效地提高低压水下井口头系统的抗疲劳能力。对深水作业时选择高低压井口头具有一定的指导意义。

关键词：水下井口头系统锁紧机构工作原理有限元分析

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2型糖尿病胰岛素治疗患者运动现状的调查研究

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中国医药科学 2023年第2期13卷 106-109页

作者：冯苏文许方蕾张丽亚赵春艳同济大学医学院上海200092 同济大学附属同济医院护理部上海200065 同济大学附属同济医院内分泌科上海200065 同济大学附属东方医院护理部上海200129

目的调查上海市2型糖尿病胰岛素治疗患者的运动现状,分析存在的问题,为指导患者开展运动管理提供依据。方法 2021年5-6月采用自制调查问卷进行便利抽样,调查上海市社区卫生服务中心和综合性医院的533例2型糖尿病胰岛素治疗患者,了解胰... 详细信息

目的调查上海市2型糖尿病胰岛素治疗患者的运动现状,分析存在的问题,为指导患者开展运动管理提供依据。方法 2021年5-6月采用自制调查问卷进行便利抽样,调查上海市社区卫生服务中心和综合性医院的533例2型糖尿病胰岛素治疗患者,了解胰岛素治疗和运动现状。结果胰岛素保存完全正确率为22.33%,胰岛素笔针头每次更换率为35.46%,胰岛素注射准时率为73.92%;调查对象中52.35%运动时长为30~60 min,32.27%运动强度为中等强度,41.65%运动频率为3~4 d/周,70.17%未进行运动前后的热身和放松,83.86%未进行运动时心率监测,56.85%运动无专业指导。结论 2型糖尿病胰岛素治疗患者运动管理不完善,运动前准备不充分,运动过程监管和运动后评价缺失。

关键词： 2型糖尿病胰岛素治疗运动疗法横断面研究

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5-HT_2C Receptor Structures Reveal the Structural Basis of GPCR Polypharmacology

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CELL 2018年第4期172卷 719-+页

作者： Peng, Yao McCorvy, John D. Harpsoe, Kasper Lansu, Katherine Yuan, Shuguang Popov, Petr Qu, Lu Pu, Mengchen Che, Tao Nikolajsen, Louise F. Huang, Xi-Ping Wu, Yiran Shen, Ling Bjorn-Yoshimoto, Walden E. Ding, Kang Wacker, Daniel Han, Gye Won Cheng, Jianjun Katritch, Vsevolod Jensen, Anders A. Hanson, Michael A. zhao, suwen Gloriam, David E. Roth, Bryan L. Stevens, Raymond C. Liu, Zhi-Jie ShanghaiTech Univ iHuman Inst Shanghai 201210 Peoples R China Kunming Med Univ Inst Mol & Clin Med Yunnan Key Lab Stem Cell & Regenerat Med Kunming 650500 Yunnan Peoples R China Chinese Acad Sci Inst Biophys Natl Lab Biomacromol Beijing 100101 Peoples R China Univ North Carolina Chapel Hill Dept Pharmacol Chapel Hill NC 27599 USA Univ Copenhagen Dept Drug Design & Pharmacol Univ Pk 2 DK-2100 Copenhagen Denmark Ecole Polytech Fed Lausanne Lab Phys Chem Polymers & Membranes CH B3 495Batiment CHStn 6 CH-1015 Lausanne Switzerland Univ Southern Calif Michelson Ctr Bridge Inst Dept Biol Sci Los Angeles CA 90089 USA Univ Southern Calif Michelson Ctr Bridge Inst Dept Chem Los Angeles CA 90089 USA Moscow Inst Phys & Technol Dolgoprudnyi 141700 Russia Univ North Carolina Chapel Hill NIMH PDSP Chapel Hill NC 27599 USA ShanghaiTech Univ Sch Life Sci & Technol Shanghai 201210 Peoples R China GPCR Consortium San Marcos CA 92078 USA Univ North Carolina Chapel Hill Eshelman Sch Pharm Div Chem Biol & Med Chem Chapel Hill NC 27599 USA

Drugs frequently require interactions with multiple targets-via a process known as poly-pharmacology-to achieve their therapeutic actions. Currently, drugs targeting several serotonin receptors, including the 5-HT2C receptor, are useful for treating obesity, drug abuse, and schizophrenia. The competing challenges of developing selective 5-HT2C receptor ligands or creating drugs with a defined poly-pharmacological profile, especially aimed at G protein-coupled receptors (GPCRs), remain extremely difficult. Here, we solved two structures of the 5-HT2C receptor in complex with the highly promiscuous agonist ergotamine and the 5-HT2A-C receptor-selective inverse agonist ritanserin at resolutions of 3.0 angstrom and 2.7 angstrom, respectively. We analyzed their respective binding poses to provide mechanistic insights into their receptor recognition and opposing pharmacological actions. This study investigates the structural basis of polypharmacology at canonical GPCRs and illustrates how understanding characteristic patterns of ligand-receptor interaction and activation may ultimately facilitate drug design at multiple GPCRs.

关键词： GPCR serotonin 2C receptor ergotamine ritanserin polypharmacology selectivity crystal structures

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剖宫产术后再次妊娠合并前置胎盘孕妇胎盘植入的风险评估及不良结局预测全国多中心回顾性研究

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中华妇产科杂志 2023年第1期58卷 26-36页

作者：白桂芹陈蔚琳黄向华赵绍杰赵淑萍陈秀娟陈素文杨华卢霞刘冠媛陈琼华张林爱金力西安交通大学第一附属医院基因联合实验室西安710061 中国医学科学院北京协和医院妇产科北京100730 河北医科大学第二医院妇产科石家庄050000 江苏省无锡市妇幼保健院妇科无锡214002 青岛大学附属妇女儿童医院妇科青岛266034 福建省妇幼保健院妇科福州350001 首都医科大学附属北京妇产医院计划生育科北京100026 天津市中心妇产科医院计划生育科天津300011 新疆医科大学附属第六医院妇产科乌鲁木齐830002 首都医科大学附属北京朝阳医院妇产科北京100020 厦门大学附属第一医院妇产科厦门361003 山西省妇幼保健院计划生育科太原030013

目的探讨剖宫产术后再次妊娠合并前置胎盘孕妇的胎盘植入性疾病(PAS)影像学诊断及血管阻断方式对妊娠结局的影响。方法采用全国多中心回顾性研究,于2018年1月1日至12月31日选取12家三级甲等医院的剖宫产术后再次妊娠合并前置胎盘的妊娠... 详细信息

目的探讨剖宫产术后再次妊娠合并前置胎盘孕妇的胎盘植入性疾病(PAS)影像学诊断及血管阻断方式对妊娠结局的影响。方法采用全国多中心回顾性研究,于2018年1月1日至12月31日选取12家三级甲等医院的剖宫产术后再次妊娠合并前置胎盘的妊娠晚期单胎妊娠孕妇共747例。采用单因素及多因素logistic回归分析,观察剖宫产术后再次妊娠合并前置胎盘孕妇严重不良结局(子宫切除、术中出血量≥1000 ml、术中诊断PAS)的危险因素;观察产前超声和磁共振成像(MRI)检查在胎盘PAS及严重不良结局预测中的作用。根据是否行血管介入(子宫动脉栓塞术或腹主动脉球囊阻断术)分为阻断组(106例)与未阻断组(641例),比较两组孕妇的母儿结局。结果(1)一般情况:747例剖宫产术后再次妊娠合并前置胎盘孕妇的子宫切除率为10.4%(78/747),术中出血量≥1000 ml者占55.8%(417/747),术中确诊PAS者占47.5%(355/747)。子宫破裂的发生率为0.8%(6/747)。(2)严重不良结局的危险因素:子宫切除的危险因素是血管阻断方式和术中出血量,子宫动脉栓塞术者子宫切除的可能性是腹主动脉球囊阻断术者的5.319倍(95%CI为1.346~21.018);术中出血量≥1000 ml的危险因素是剖宫产术次数、超声检查提示PAS及可疑PAS、术中是否诊断PAS、完全性前置胎盘;术中诊断PAS的危险因素是子宫瘢痕厚度、超声检查提示PAS及可疑PAS、MRI检查提示PAS及可疑PAS、完全性前置胎盘;(3)超声和MRI检查在预测PAS中的作用:超声检查预测PAS的敏感度为47.5%,特异度为88.4%;kappa值为0.279(P<0.001),一致性一般。MRI检查预测PAS的敏感度为79.2%,特异度为97.8%;kappa值为0.702(P<0.001),一致性较好。超声且MRI检查均提示PAS孕妇的术中出血量及子宫切除率均显著高于仅超声或仅MRI检查提示PAS的孕妇。(4)血管阻断对妊娠结局的影响:阻断组与未阻断组孕妇的术中出血量、术中出血量≥1000 ml发生率分别比较,差异均无统计学意义(P均>0.05)。阻断组腹主动脉球囊阻断者、子宫动脉栓塞者与未阻断组孕妇的术中出血量无显著差异(P=0.409);阻断组子宫动脉栓塞孕妇的子宫切除率显著高于腹主动脉球囊阻断孕妇[分别为39.3%(22/56)、10.0%(5/50),P=0.001]。结论剖宫产术后再次妊娠合并前置胎盘的妊娠晚期孕妇,MRI检查比超声检查预测PAS的一致性好,超声检查结合MRI检查可有效地预测子宫切除率和术中出血量。血管阻断不能降低术中出血量,子宫动脉栓塞孕妇的子宫切除率高于腹主动脉球囊阻断孕妇。

关键词：剖宫产术前置胎盘失血,手术侵入性胎盘子宫切除术回顾性研究

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黄河流域十大孔兑野生种子植物区系组成及特征研究

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广东水利水电 2023年第9期 44-49页

作者：史世斌李素文李婉娇钟鑫鑫赵宏胜内蒙古自治区水利科学研究院呼和浩特010052 宁城县水利局内蒙古宁城024299 国网内蒙古东部电力有限公司呼和浩特010010 内蒙古农业大学沙漠治理学院呼和浩特010018

由于十大孔兑所处地理位置特殊(上游为丘陵沟壑区,中游为风沙区,下游为平原区),导致其生态类型复杂多样,而植被是表观生态系统特征的重要组分。该文进行大量野外实地调查并结合文献查阅,对十大孔兑野生种子植物进行调查、统计,同时对区... 详细信息

由于十大孔兑所处地理位置特殊(上游为丘陵沟壑区,中游为风沙区,下游为平原区),导致其生态类型复杂多样,而植被是表观生态系统特征的重要组分。该文进行大量野外实地调查并结合文献查阅,对十大孔兑野生种子植物进行调查、统计,同时对区域内植物的种类组成、水分生态类型、生活型以及种的地理成分和资源价值进行分析。结果表明:①十大孔兑地域共有野生种子植物278种,隶属于55科,180属;其中裸子植物3科,3属,4种;被子植物52科,177属,274种。②植物水分生态类型以中生植物为主;生活型以草本植物为主,区系中种的地理成分较为复杂,以东古北极分布种最多,反映了研究植物区域生境类型多样及地域性强的特征。③植物资源价值以药用植物资源较丰富,其次是植物蛋白和氨基酸植物资源。珍稀濒危保护植物5种,其中国家Ⅱ级保护植物3种。十大孔兑位于黄河流域中上游,关乎黄河流域安全发展,而植被特征可以直接反映生态系统的稳定性,在今后对十大孔兑生态评价过程中,可以定期进行植被情况调查,据此科学地判断十大孔兑生态恢复境况,并借此完善黄河流域种子植物资源数据库。

关键词：十大孔兑种子植物生活型水分生态型地理成分资源价值

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Crystal structure of the Frizzled 4 receptor in a ligand-free state

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NATURE 2018年第7720期560卷 666-+页

作者： Yang, Shifan Wu, Yiran Xu, Ting-Hai de Waal, Parker W. He, Yuanzheng Pu, Mengchen Chen, Yuxiang DeBruine, Zachary J. Zhang, Bingjie Zaidi, Saheem A. Popov, Petr Guo, Yu Han, Gye Won Lu, Yang Suino-Powell, Kelly Dong, Shaowei Harikumar, Kaleeckal G. Miller, Laurence J. Katritch, Vsevolod Xu, H. Eric Shui, Wenqing Stevens, Raymond C. Melcher, Karsten zhao, suwen Xu, Fei ShanghaiTech Univ iHuman Inst Shanghai Peoples R China Van Andel Res Inst Innovat & Integrat Program Ctr Canc & Cell Biol Grand Rapids MI USA Harbin Inst Technol Sch Life Sci & Technol Ctr Life Sci Harbin Heilongjiang Peoples R China ShanghaiTech Univ Sch Life Sci & Technol Shanghai Peoples R China Chinese Acad Sci Shanghai Inst Biol Sci Inst Biochem & Cell Biol Shanghai Peoples R China Univ Chinese Acad Sci Beijing Peoples R China Univ Southern Calif Dept Biol Sci Bridge Inst Los Angeles CA 90089 USA Univ Southern Calif Dept Chem Bridge Inst Los Angeles CA 90089 USA Moscow Inst Phys & Technol Dolgoprudnyi Russia Mayo Clin Dept Mol Pharmacol & Expt Therapeut Scottsdale AZ USA Chinese Acad Sci Shanghai Inst Mat Med VARI SIMM Ctr Key Lab Receptor Res Shanghai Peoples R China Chinese Acad Sci Shanghai Inst Mat Med Ctr Struct & Funct Drug Targets Shanghai Peoples R China

Frizzled receptors (FZDs) are class-F G-protein-coupled receptors (GPCRs) that function in Wnt signalling and are essential for developing and adult organisms(1,2). As central mediators in this complex signalling pathway, FZDs serve as gatekeeping proteins both for drug intervention and for the development of probes in basic and in therapeutic research. Here we present an atomic-resolution structure of the human Frizzled 4 receptor (FZD4) transmembrane domain in the absence of a bound ligand. The structure reveals an unusual transmembrane architecture in which helix VI is short and tightly packed, and is distinct from all other GPCR structures reported so far. Within this unique transmembrane fold is an extremely narrow and highly hydrophilic pocket that is not amenable to the binding of traditional GPCR ligands. We show that such a pocket is conserved across all FZDs, which may explain the long-standing difficulties in the development of ligands for these receptors. Molecular dynamics simulations on the microsecond timescale and mutational analysis uncovered two coupled, dynamic kinks located at helix VII that are involved in FZD4 activation. The stability of the structure in its ligand-free form, an unfavourable pocket for ligand binding and the two unusual kinks on helix VII suggest that FZDs may have evolved a novel ligand-recognition and activation mechanism that is distinct from that of other GPCRs.

关键词： Cell signalling X-ray crystallography

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新疆红狐体表犬硬蜱的分子生物学和雄蜱形态学研究

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中国寄生虫学与寄生虫病杂志 2023年第6期41卷 739-743页

作者：李逢时赵姗姗谭文波吾热力哈孜·哈孜汗谷新利王素文刘钢王远志石河子大学医学院新疆石河子832003 石河子大学动物科技学院新疆石河子832003

目的对来源于新疆博尔塔拉蒙古自治州和昌吉回族自治州的野外红狐尸首体表的疑似硬蜱进行形态学和分子生物学鉴定。方法于2018—2019年采集新疆博尔塔拉蒙古自治州和昌吉回族自治州的野外红狐尸首体表的硬蜱,体视显微镜进行形态学观察... 详细信息

目的对来源于新疆博尔塔拉蒙古自治州和昌吉回族自治州的野外红狐尸首体表的疑似硬蜱进行形态学和分子生物学鉴定。方法于2018—2019年采集新疆博尔塔拉蒙古自治州和昌吉回族自治州的野外红狐尸首体表的硬蜱,体视显微镜进行形态学观察。选取5只代表性成蜱(3♂,2♀),分别提取DNA,PCR扩增线粒体基因片段16S rDNA并测序,序列采用BLAST进行比对,采用最大似然法建立系统进化树,利用MEGA 7.0软件进行同源性分析。结果共采集38只硬蜱,雌雄分别36、2只。镜下可见蜱假头基明显,呈孔区卵圆形,须肢和口下板细长,排列不紧密,口下板长度和假头基宽度大约相等,气门板呈圆形,中心凹陷,盾板无缘垛,无花斑,无眼,肛沟为前沟型,足表面覆盖棕红色釉质,相对纤细,体表附大量细毛。雌蜱盾板覆盖盾板的前半部,生殖孔边缘有生殖帷;雄蜱盾板全部覆盖,肛沟为前沟型,肛侧板和肛板明显,与犬硬蜱雄蜱的形态特点相符。PCR扩增结果显示,5只蜱DNA均扩增出460 bp的目的条带。序列分析结果显示,5只代表蜱的16S rDNA序列相同,与中国新疆塔里木盆地来源的犬硬蜱(GenBank登录号为MG651937)序列一致性高达99.47%,与英国、德国和法国来源的犬硬蜱(GenBank登录号分别为KY962071、KY962069、KY962074)序列一致性均为99.20%。系统进化树分析结果显示,5只代表蜱的序列与中国新疆和法国来源的犬硬蜱聚为一支,与嗜鸟硬蜱、青紫硬蜱有较近的亲缘关系。结论应用形态学和分子生物学相结合的方法鉴定了新疆红狐体表的犬硬蜱,该犬硬蜱与欧洲来源的犬硬蜱有较高同源性。

关键词：犬硬蜱红狐形态学分子生物学新疆

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Wireless Monitoring of Small Strains in Intelligent Robots via a Joule Heating Effect in Stretchable Graphene-Polymer Nanocomposites

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ADVANCED FUNCTIONAL MATERIALS 2020年第13期30卷 1910809-1910809页

作者： Zhang, Ding Xu, suwen zhao, Xue Qian, Weiqi Bowen, Chris R. Yang, Ya Chinese Acad Sci Beijing Inst Nanoenergy & Nanosyst Beijing Key Lab Micronano Energy & Sensor CAS Ctr Excellence Nanosci Beijing 100083 Peoples R China Univ Chinese Acad Sci Sch Nanosci & Technol Beijing 100049 Peoples R China Univ Bath Dept Mech Engn Bath BA2 7AK Avon England Guangxi Univ Sch Phys Sci & Technol Ctr Nanoenergy Res Nanning 530004 Peoples R China

Flexible strain sensors are an important component for future intelligent robotics. However, the majority of current strain sensors must be electrically connected to a corresponding monitoring system via conducting wires, which increases system complexity and restricts the working environment for monitoring strains. Here, stretchable graphene-polymer nanocomposites that act as strain sensors using a Joule heating effect are reported. When the resistance of the sensor changes in response to a strain, the resulting change in temperature is wirelessly detected in an intelligent robot. By engineering and optimizing the surface structure of graphene-polymer nanocomposites, the fabricated strain sensors exhibit excellent stability when subjected to periodic temperature signals over 400 cycles while being periodically strained and deliver a high strain sensitivity of 7.03 x 10(-4) degrees C-1 %(-1) for strain levels of 0% to 30%. As a wearable electronic device, the approach provides the capability to wirelessly monitor small strains for intelligent robots at a high strain resolution of approximate to 0.1%. Moreover, when the strain sensing system operates as a multichannel structure, it allows precise strain detection simultaneously, or in sequence, for each finger of an intelligent robot.

关键词： graphene-polymer joule heating effects strain sensors stretchable sensors wireless monitoring

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