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Synthesis of monoclinic BiVO₄ microribbons by sol-gel combined with electrospinning process and photocatalytic degradation performances

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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY 2014年第1期70卷 24-32页

作者： Liu, Guoshuai Liu, suwen Lu, Qifang Sun, Haiyan Xu, Fengxiu zhao, Gang Qilu Univ Technol Sch Mat Sci & Engn Jinan 250353 Peoples R China

Quasi one-dimensional (1D) BiVO4 microribbons were prepared by sol-gel combined with electrospinning method. The prepared samples were characterized with thermogravimeter, differential scanning calorimeter, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, transmission electron microscopy and UV-vis absorbance spectroscopy. The as-prepared products showed a well-defined ribbon structure with 2-3 mu m in width, tens of microns in length and about 0.4 mu m in thickness, respectively and exhibited excellent photocatalytic activity in the photodegradation of methylene blue under visible light irradiation.

关键词： BiVO4 Electrospinning Microribbon Photocatalytic activity

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Molecular Evolution of a Subgroup of the WRKY Transcription Factor Family in Gramineae

Molecular Evolution of a Subgroup of the WRKY Transcription ...

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第一届全国玉米生物学学术研讨会

作者：金晶 Haiyang Jiang Lingyan Zhou Yang zhao Zhen Li suwen Zhu Beijiu Cheng Key Laboratory of Crop Biology of Anhui Province Anhui Agricultural University

The WRKY gene family,which encode proteins in the regulation processes of diverse developmental stages,is one of the largest families of transcription factors in higher *** I genes of WRKY family(WRKY I)serve as the node for signal transduction,and play important roles in the response to abiotic stress in *** with those researches in function,molecular evolutionary mechanism in

关键词： Molecular Evolution of a Subgroup of the WRKY Transcription Factor Family in Gramineae

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Molecular Evolution of a Subgroup of the WRKY Transcription Factor Family in Gramineae

Molecular Evolution of a Subgroup of the WRKY Transcription ...

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第一届全国玉米生物学学术研讨会

作者： Jing Jin Haiyang Jiang Lingyan Zhou Yang zhao Zhen Li suwen Zhu Beijiu Cheng Key Laboratory of Crop Biology of Anhui Province Anhui Agricultural UniversityHefeiChina

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软枣猕猴桃嫩枝扦插繁育技术

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吉林林业科技 2015年第3期44卷 54-55页

作者：庞素文赵杨吉林省林业调查规划院吉林长春130022 淄博市野生动植物保护站山东淄博255095

在伏天，选择结果多的枝干上当年萌生的半木质化枝条做插穗，经过50×10－6的萘乙酸溶液浸泡2 h，以木屑、园田土、细河沙（3：1：1）为基质进行插扦育苗，20 d左右可见叶芽膨大，插穗基部愈伤组织开始形成；40 d左右叶芽开始萌动... 详细信息

在伏天，选择结果多的枝干上当年萌生的半木质化枝条做插穗，经过50×10－6的萘乙酸溶液浸泡2 h，以木屑、园田土、细河沙（3：1：1）为基质进行插扦育苗，20 d左右可见叶芽膨大，插穗基部愈伤组织开始形成；40 d左右叶芽开始萌动并逐渐展叶，插穗基部长出1～2条新根，生根率可达90％以上。

关键词：软枣猕猴桃扦插繁育技术

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Identification and characterization of the RCI2 gene family in maize (Zea mays)

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JOURNAL OF GENETICS 2014年第3期93卷 655-666页

作者： zhao, Yang Tong, Haiqing Cai, Ronghao Peng, Xiaojian Li, Xiaoyu Gan, Defang Zhu, suwen Anhui Agr Univ Sch Life Sci Key Lab Crop Biol Anhui Prov Hefei 230036 Peoples R China Anhui Agr Univ Sch Hort Hefei 230036 Peoples R China

Rare-cold-inducible (RCI2) genes are structurally conserved members that encode small, highly hydrophobic proteins involved in response to various abiotic stresses. Phylogenetic and functional analyses of these genes have been conducted in Arabidopsis, but an extensive investigation of the RCI2 gene family has not yet been carried out in maize. In the present study, 10 RCI2 genes were identified in a fully sequenced maize genome. Structural characterization and expression pattern analysis of 10 ZmRCI2s (Zea mays RCI2 genes) were subsequently determined. Sequence and phylogenetic analyses indicated that ZmRCI2s are highly conserved, and most of them could be grouped with their orthologues from other organisms. Chromosomal location analysis indicated that ZmRCI2s were distributed unevenly on seven chromosomes with two segmental duplication events, suggesting that maize RCI2 gene family is an evolutionarily conserved family. Putative stress-responsive cis-elements were detected in the 2-kb promoter regions of the 10 ZmRCI2s. In addition, the 10 ZmRCI2s showed different expression patterns in maize development based on transcriptome analysis. Further, microarray and quantitative real-time PCR (qRT-PCR) analysis showed that each maize RCI2 genes were responsive to drought stress, suggesting their important roles in drought stress response. The results of this work provide a basis for future cloning and application studies of maize RCI2 genes.

关键词： maize RCI2 gene phylogenetic relationship gene expression drought

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New strategy for surface functionalization of periodic mesoporous silica based on meso-HSiO1.5

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Journal of the American Chemical Society 2014年第4期136卷 1178-1181页

作者： Xie, Zhuoying Bai, Ling Huang, suwen Zhu, Cun zhao, Yuanjin Gu, Zhong-Ze State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 China Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou 1 Suzhou 215123 China

Organic functionalization of periodic mesoporous silicas (PMSs) offers a way to improve their excellent properties and wide applications owing to their structural superiority. In this study, a new strategy for organic functionalization of PMSs is demonstrated by hydrosilylation of the recently discovered "impossible" periodic mesoporous hydridosilica, meso-HSiO1.5. This method overcomes the disadvantages of present pathways for organic functionalization of PMSs with organosilica. Moreover, compared to the traditional functionalization on the surface of porous silicon by hydrosilylation, the template-synthesized meso-HSiO1.5 is more flexible to access functional-groups-loaded PMSs with adjustable microstructures. The new method and materials will have wider applications based on both the structure and surface superiorities. © 2014 American Chemical Society.

关键词： Hydrosilylation

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共射-共集反馈放大器高频响应仿真与分析

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淮北师范大学学报（自然科学版） 2016年第3期37卷 38-42页

作者：赵鑫王江涛杨一军陈得宝李峥方振国李素文淮北师范大学物理与电子信息学院安徽淮北235000

对共射-共集电压并联负反馈放大电路采用方框图法计算基本放大器和反馈放大器的源互阻增益,结果的正确性经高频小信号等效电路在EWB软件下的仿真和MATLAB结点电压法下的数值解得以验证.软件分析显示,开、闭环的上限频率之比是反馈放大... 详细信息

对共射-共集电压并联负反馈放大电路采用方框图法计算基本放大器和反馈放大器的源互阻增益,结果的正确性经高频小信号等效电路在EWB软件下的仿真和MATLAB结点电压法下的数值解得以验证.软件分析显示,开、闭环的上限频率之比是反馈放大器的源反馈深度,满足单极点开、闭环上限频率间关系,说明电路中有上限频率远小于其它的主极点.采用开路时间常数法讨论下各结电容对上限频率的影响,得到无论开、闭环状态,容量较小的共射电路集电结电容都因回路电阻大,造成极点上限频率低,在频率响应中起主要作用的结论.以上分析方法可用于讨论多极点系统中的各极点频率,以便针对性调整电路参数,改善系统频率特性.

关键词：共射-共集上限频率 MATLAB EWB

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根皮苷对雌性果蝇寿命的影响及其作用机制

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食品科学 2015年第7期36卷 167-171页

作者：赵江陈纯韩晓东刘素稳马娜王浩天津科技大学食品工程与生物技术学院天津300457 天津津酒集团天津300131 河北科技师范学院食品科技学院河北秦皇岛066600 天津科技大学生物工程学院天津300457

目的:研究根皮苷(phloridzin)对雌性果蝇寿命的影响并探究其延缓果蝇衰老的分子机制。方法:在果蝇寿命实验中,采用2日龄雌性果蝇作为研究对象,在其培养基中分别加入0、0.5、2.0、5.0 mg/m L根皮苷进行培养,直至果蝇全部死亡,计算果蝇的... 详细信息

目的:研究根皮苷(phloridzin)对雌性果蝇寿命的影响并探究其延缓果蝇衰老的分子机制。方法:在果蝇寿命实验中,采用2日龄雌性果蝇作为研究对象,在其培养基中分别加入0、0.5、2.0、5.0 mg/m L根皮苷进行培养,直至果蝇全部死亡,计算果蝇的平均寿命和最长寿命;通过白草枯和双氧水急性实验测定根皮苷对果蝇氧化应激损伤的影响;试剂盒法测定果蝇体内抗氧化酶活力;实时荧光定量聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)检测抗氧化基因表达水平。结果:2.0、5.0 mg/m L根皮苷可以显著延长果蝇的平均寿命和最长寿命(P<0.05);根皮苷能够对百草枯和双氧水造成的雌性果蝇氧化应激损伤起到有效保护作用;添加5.0 mg/m L根皮苷可以极显著提高Cu/Zn-超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活力(P<0.01),极显著降低丙二醛(malonaldehyde,MDA)含量(P<0.01);添加5.0 mg/m L根皮苷可以极显著上调Cu/Zn-SOD及CAT m RNA表达水平(P<0.01)。结论:根皮苷能够延缓雌性果蝇衰老,且具有抗氧化作用,这可能与其调控抗氧化基因的表达水平相关。

关键词：果蝇根皮苷寿命抗氧化酶活力基因表达

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Discovery of new enzymes and metabolic pathways by using structure and genome context

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NATURE 2013年第7473期502卷 698-+页

作者： zhao, suwen Kumar, Ritesh Sakai, Ayano Vetting, Matthew W. Wood, B. McKay Brown, Shoshana Bonanno, Jeffery B. Hillerich, Brandan S. Seidel, Ronald D. Babbitt, Patricia C. Almo, Steven C. Sweedler, Jonathan V. Gerlt, John A. Cronan, John E. Jacobson, Matthew P. Univ Calif San Francisco Dept Pharmaceut Chem San Francisco CA 94143 USA Univ Illinois Inst Genom Biol Urbana IL 61801 USA Albert Einstein Coll Med Dept Biochem Bronx NY 10461 USA Univ Calif San Francisco Dept Bioengn & Therapeut Sci San Francisco CA 94143 USA Univ Illinois Dept Chem Urbana IL 61801 USA Univ Illinois Dept Biochem Urbana IL 61801 USA Univ Illinois Dept Microbiol Urbana IL 61801 USA

Assigning valid functions to proteins identified in genome projects is challenging: overprediction and database annotation errors are the principal concerns(1). We and others(2) are developing computation-guided strategies for functional discovery with 'metabolite docking' to experimentally derived(3) or homology-based(4) three-dimensional structures. Bacterial metabolic pathways often are encoded by 'genome neighbourhoods' (gene clusters and/or operons), which can provide important clues for functional assignment. We recently demonstrated the synergy of docking and pathway context by 'predicting' the intermediates in the glycolytic pathway in Escherichia coli(5). Metabolite docking to multiple binding proteins and enzymes in the same pathway increases the reliability of in silico predictions of substrate specificities because the pathway intermediates are structurally similar. Here we report that structure-guided approaches for predicting the substrate specificities of several enzymes encoded by a bacterial gene cluster allowed the correct prediction of the in vitro activity of a structurally characterized enzyme of unknown function (PDB 2PMQ), 2-epimerization of trans-4-hydroxy-L-proline betaine (tHyp-B) and cis-4-hydroxy-D-proline betaine (cHyp-B), and also the correct identification of the catabolic pathway in which Hyp-B 2-epimerase participates. The substrate-liganded pose predicted by virtual library screening (docking) was confirmed experimentally. The enzymatic activities in the predicted pathway were confirmed by in vitro assays and genetic analyses;the intermediates were identified by metabolomics;and repression of the genes encoding the pathway by high salt concentrations was established by transcriptomics, confirming the osmolyte role of tHyp-B. This study establishes the utility of structure-guided functional predictions to enable the discovery of new metabolic pathways.

关键词： Bacteria/enzymology Bacteria/genetics Bacteria/metabolism Bacterial Proteins/chemistry Bacterial Proteins/genetics Bacterial Proteins/metabolism Enzymes/chemistry Enzymes/genetics Enzymes/metabolism Gene Expression Profiling Genes, Bacterial/genetics Genes, Bacterial/genetics Genome, Bacterial/genetics Genome, Bacterial/genetics Glycolysis Kinetics Metabolic Networks and Pathways/genetics Metabolism Metabolomics Models, Molecular Molecular Sequence Annotation/methods Multigene Family/genetics Operon Operon Structural Homology, Protein Substrate Specificity

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Sesamin ameliorates doxorubicin-induced cardiotoxicity: Involvement of Sirt1 and Mn-SOD pathway

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TOXICOLOGY LETTERS 2014年第2期224卷 257-263页

作者： Su, suwen Li, Qian Liu, Yi Xiong, Chen Li, Junxia Zhang, Rong Niu, Yujie zhao, Lijuan Wang, Yongli Guo, Huicai Hebei Med Univ Key Lab Neural & Vasc Biol Minist Educ Dept Pharmacol Shijiazhuang 050017 Hebei Province Peoples R China Hebei Med Univ Dept Physiol Shijiazhuang 050017 Hebei Province Peoples R China Hebei Med Univ Dept Toxicol Shijiazhuang 050017 Hebei Province Peoples R China

Oxidative stress caused by doxorubicin (DOX) is believed to be a major underlying molecular mechanism of DOX-induced cardiotoxicity. Sesamin (Ses), an active component extracted from sesame seeds, exhibits antioxidative and anti-inflammatory effects. In the present study, possible protective mechanisms of Ses on DOX-induced cardiotoxicity were investigated in rats and cultured H9C2 cells. We demonstrated that Ses exhibits a significant protective effect on cardiac tissue in animal and cell models of DOX-induced cardiac injury. Moreover, Ses can ameliorate DOX-induced oxidative stress and mitochondrial damage. Further studies suggested that Ses is able to up-regulate the protein expression of Mn-SOD in normal rats and to restore the decreased expression of Mn-SOD in DOX-induced cardiac injury rats. Exposure to Ses or DOX alone slightly increased the protein expression of Sirt1;however, a more remarkable increase in Sirt1 protein level was detected in the Ses + DOX group. Treatment with a pan-sirtuin inhibitor (nicotinamide) or a Sirt1-specific inhibitor (EX-527) partially antagonised the effect of Ses on DOX-induced mitochondrial damage and completely abolished the effect of Ses on Mn-SOD expression. These findings indicate that the protective mechanisms of Ses on DOX-induced cardiotoxicity are involved in the alleviation of oxidative stress injury and Mn-SOD dysfunction, partially via the activation of Sirt1. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

关键词： Doxorubicin Sesamin Mn-SOD Sirt1 Cardiotoxicity

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