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经皮穴位电刺激对行脊柱开放性手术的老年患者围术期神经认知障碍及临床预后的影响

温州医科大学学报 2024年第1期54卷 1-6页

作者：余露钱旭雯李国艳汤雯倩李春黇刘国凯北京中医药大学东直门医院麻醉科北京100700 北京中医药大学东方医院麻醉科北京100078 北京中医药大学东直门医院针灸科北京100700

目的:探讨经皮穴位电刺激(TEAS)合谷、内关二穴辅助全身麻醉对老年脊柱开放性手术患者围术期神经认知障碍(PND)及临床预后的影响。方法:选取2020年8月至2022年3月北京中医药大学东直门医院骨科脊柱开放性手术患者为研究对象。采取随机... 详细信息

目的:探讨经皮穴位电刺激(TEAS)合谷、内关二穴辅助全身麻醉对老年脊柱开放性手术患者围术期神经认知障碍(PND)及临床预后的影响。方法:选取2020年8月至2022年3月北京中医药大学东直门医院骨科脊柱开放性手术患者为研究对象。采取随机对照原则,将符合纳入标准的64例患者随机分为单纯全身麻醉组(对照组)和穴位电刺激复合全身麻醉组(实验组),每组32例。实验组在麻醉诱导前30 min直至手术结束时选择合谷穴、内关穴与神经刺激仪连接,给予电刺激。对照组在合谷和内关两穴贴电极片并与神经刺激仪连接,但不给予电刺激,其余治疗同实验组。观察术后7 d谵妄(POD)的发生率,在排除POD的情况下30 d内神经认知恢复延迟(DNR)的发生率,在麻醉前、气管插管时、切皮时、置入椎弓根钉时、术毕时患者脑电双频谱指数(BIS)值的变化,以及30 d内术后并发症(切口感染、恶心呕吐)发生情况。结果:64例患者均顺利完成手术。两组患者的基线值差异无统计学意义(P>0.05)。术毕时,实验组BIS值高于对照组(P<0.05);实验组术后30 d内的DNR发生率低于对照组(P<0.05);术后30 d内呕吐例数实验组低于对照组,差异有统计学意义(P<0.05)。结论:TEAS与静脉全身麻醉结合用于老年人脊柱开放性手术可降低PND的发生率及术后呕吐发生率,加快苏醒,改善患者预后。

关键词：经皮穴位电刺激简易精神状态检测量表老年脊柱开放手术临床预后围术期神经认知障碍

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Free-standing polydopamine films generated in the presence of different metallic ions: the comparison of reaction process and film properties

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RSC ADVANCES 2018年第33期8卷 18347-18354页

作者： Han, xuwen tang, Feng Jin, Zhaoxia Renmin Univ China Dept Chem Beijing 100872 Peoples R China

Polydopamine is widely used in surface modification, nanofiltration, photonic devices and drug delivery. The formation mechanism and properties of polydopamine are modified by the experimental conditions. Herein we demonstrated a comparison study of free-standing polydopamine films generated at the air-solution interface and their corresponding nanoparticles in solutions, in the presence of various metallic cations, Na+, Ca2+, Mg2+ and Co2+. Adding metallic ions influenced the intermediates in dopamine polymerization, and in turn the morphology and properties of the produced free-standing polydopamine films. Moreover, we observed that the polymerization process accompanying the stratification determines the formation of free-standing films at the air-solution interface: the fast polymerization of dopamine in a Co2+ environment leads to a rugged film surface and porous film body, whereas the comparatively slow polymerization of dopamine under conditions of other metallic ions results in a smooth and solid film. In addition, the water contact angles of the upper and lower surface of the polydopamine films were different. This investigation enriches our knowledge of dopamine polymerization in different environments, which is particularly useful for further application of free-standing polydopamine films.

关键词： Polymerization

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活血通降方对反流性食管炎模型大鼠食管动力、血清炎症因子及食管下括约肌SCF/c-kit信号通路的影响

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中医杂志 2022年第3期63卷 269-275页

作者：刘琰唐艳萍刘磊李培彩刘茜刘思雨郝旭雯杨磊天津中医药大学研究生院天津市301617 天津市南开医院

目的探讨活血通降方治疗反流性食管炎的可能作用机制。方法将60只大鼠随机分为空白组、模型组及活血通降方低、高剂量组,每组15只。除空白组外,其余各组均采用改良部分贲门肌切开术+外置幽门部分结扎术制备反流性食管炎模型大鼠。造模... 详细信息

目的探讨活血通降方治疗反流性食管炎的可能作用机制。方法将60只大鼠随机分为空白组、模型组及活血通降方低、高剂量组,每组15只。除空白组外,其余各组均采用改良部分贲门肌切开术+外置幽门部分结扎术制备反流性食管炎模型大鼠。造模成功后当天开始给药,活血通降方低、高剂量组给药量分别为3.68、7.35 g/(kg·d),空白组和模型组给予生理盐水5 ml灌胃,均连续14天。检测食管下括约肌平均肌张力变化率和平均振幅变化率、胃重量及胃排空率。HE染色观察大鼠食管组织病理形态学变化,并进行食管黏膜病理评分;测定血清中炎症因子白细胞介素8(IL-8)和肿瘤坏死因子α(TNF-α)浓度;免疫荧光法测定Cajal间质细胞(ICCs)数量;免疫印记法检测食管下括约肌中酪氨酸激酶受体(c-kit)、干细胞因子(SCF)、亮氨酸拉链和不育-α基序激酶(ZAK)和磷酸化细胞外信号调节激酶(p-ERK)表达。结果与空白组比较,模型组食管下括约肌平均肌张力变化率和平均振幅变化率、胃排空率、ICCs阳性细胞数、c-kit和SCF表达均降低,胃重量、食管黏膜损伤病理评分、IL-8和TNF-α浓度、ZAK和p-ERK表达均升高(P<0.05或P<0.01)。与模型组相比,活血通降方高剂量组食管下括约肌平均肌张力变化率和平均振幅变化率升高(P<0.01),活血通降方低、高剂量组胃排空率、ICCs阳性细胞数、c-kit和SCF表达均升高,胃重量、食管黏膜病理评分、IL-8和TNF-α浓度、ZAK和p-ERK表达均降低(P<0.05或P<0.01)。活血通降方高剂量组食管下括约肌平均肌张力变化率、平均振幅变化率、ICCs阳性细胞数均高于活血通降方低剂量组(P<0.05或P<0.01)。结论活血通降方可能通过降低食管下括约肌中ZAK表达,抑制p-ERK表达,一方面激活SCF/c-kit信号通路,促进食管及胃动力;另一方面降低血清中IL-8和TNF-α浓度改善食管下括约肌黏膜炎症,从而治疗反流性食管炎,且以高剂量效果更好。

关键词：反流性食管炎活血通降方食管下括约肌 SCF/c-kit信号通路炎症因子食管动力 Cajal间质细胞

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Novel androgen receptor antagonist identified by structure-based virtual screening, structural optimization, and biological evaluation

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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY 2020年 192卷 112156-000页

作者： tang, Qin Fu, Weitao Zhang, Minkui Wang, Ercheng Shan, Lvhu Chai, Xin Pang, Jinping Wang, xuwen Xu, Xiaohong Xu, Lei Li, Dan Sheng, Rong Hou, Tingjun Zhejiang Univ Coll Pharmaceut Sci Hangzhou 310058 Zhejiang Peoples R China Chinese Acad Sci Univ Chinese Acad Sci Zhejiang Canc Hosp Inst Canc Res & Basic Med SciCanc Hosp Hangzhou 310022 Zhejiang Peoples R China Jiangsu Univ Technol Sch Elect & Informat Engn Inst Bioinformat & Med Engn Changzhou 213001 Jiangsu Peoples R China

Androgen receptor (AR) plays important roles in the development of prostate cancer (PCa), and therefore it has been regarded as the most important therapeutic target for both hormone-sensitive prostate cancer (HSPC) and advanced PCa. In this study, a novel hit (C18) with IC50 of 2.4 mu M against AR transcriptional activity in LNCaP cell was identified through structure-based virtual screening based on molecular docking and free energy calculations. The structure-activity relationship analysis and structural optimization of C18 resulted in the discovery of a structural analogue (AT2), a more potent AR antagonist with 16-fold improved anti-AR potency. Further assays indicated that AT2 was capable of effectively inhibiting the transcriptional function of AR and blocking the nuclear translocation of AR like the second-generation AR antagonists. The antagonists discovered in this study may be served as the promising lead compounds for the development of AR-driven PCa therapeutics. (c) 2020 Elsevier Masson SAS. All rights reserved.

关键词： Prostate cancer Androgen receptor Structure-based virtual screening Antagonist Molecular docking

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Synthesis and bioactivity of 1,3-thiazolidine-2-thione derivatives against type III secretion system of Xanthomonas oryzae

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BIOORGANIC & MEDICINAL CHEMISTRY 2019年第15期27卷 3364-3371页

作者： Tao, Hui Tian, Hao Jiang, Shan Xiang, xuwen Ahmed, Wasim tang, Riyuan Cui, Zi-Ning South China Agr Univ Integrat Microbiol Res Ctr State Key Lab Conservat & Utilizat Subtrop Agrobi Guangdong Prov Key Lab Microbial Signals & Dis Co Guangzhou 510642 Guangdong Peoples R China South China Agr Univ Dept Appl Chem Coll Mat & Energy Guangzhou 510642 Guangdong Peoples R China

Targeting virulence factors of bacterial without affecting their growth and survival, has been an initiative strategy for the development of novel anti-microbial agents. The type III secretion system (T3SS), one of essential and highly conserved virulence factors in most Gram-negative pathogenic bacteria, has been regarded as an effective target that developed new anti-microbial drugs. Xanthomonas oryzae pv. oryzae (Xoo) is one of the most important bacterial pathogens on rice, which causes leaf blight disease. To discover potential anti-virulence agents against the pathogens, a new series of 1,3-thiazolidine-2-thione derivatives containing 5-phenyl-2-furan were designed and synthesized. Their structures were characterized by H-1 NMR, C-13 NMR, MS, and elemental analysis. All the title compounds inhibited the promoter activity of a harpin gene hpa1, significantly, that were further checked for the impact on bacterial growth. The results indicated that treatment of Xoo with the title compound III-7 did not affect bacterial growth or survival. Moreover, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that the expression of the Xoo T3SS was suppressed by treatment with the inhibitor. The mRNA levels of representative genes in the hrp (hypersensitive response and pathogenicity) cluster, as well as the regulatory genes hrpG and hrpX, were reduced. Finally, the in vivo test demonstrated that the compounds could reduce the disease symptoms of Xoo on the rice cultivar (Oryza sativa) IR24.

关键词： Xanthomonas oryzae pv. oryzae (Xoo) Type III secretion system (T3SS) Small molecule inhibitors Virulence factors 1,3-Thiazolidine-2-thione

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HKUST封装磷钼酸催化乙二醇与异丁烯选择性醚化的研究

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南京工业大学学报（自然科学版） 2022年第2期44卷 141-147页

作者：蒙旭雯李纪元周广飞张竹修汤吉海崔咪芬乔旭南京工业大学化工学院材料化学工程国家重点实验室江苏南京211800

采用一锅法制备金属有机框架材料HKUST封装不同含量磷钼酸的HPMo@HKUST催化剂,通过X线衍射、傅里叶变换红外光谱、N2吸附脱附、吡啶红外光谱、热分析等手段对催化剂的晶型、结构、孔径、酸位、热稳定性等进行表征,考察HPMo@HKUST在乙二... 详细信息

采用一锅法制备金属有机框架材料HKUST封装不同含量磷钼酸的HPMo@HKUST催化剂,通过X线衍射、傅里叶变换红外光谱、N2吸附脱附、吡啶红外光谱、热分析等手段对催化剂的晶型、结构、孔径、酸位、热稳定性等进行表征,考察HPMo@HKUST在乙二醇与异丁烯选择性醚化制备乙二醇单叔丁基醚反应中的催化性能并进行工艺优化。结果表明:HPMo@HKUST催化剂成功封装磷钼酸,在维持HKUST晶型结构的同时,提高了催化剂的稳定性,且引入的Brönsted酸位能提高催化乙二醇与异丁烯选择性醚化的催化活性与产物选择性。乙二醇单叔丁基醚的选择性随着磷钼酸负载量的增加先降低后升高。10%HPMo@HKUST催化剂在异丁稀与乙二醇摩尔比6、催化剂用量占乙二醇质量的30%、100℃反应1 h条件下,乙二醇转化率为88%,乙二醇单叔丁基醚选择性为81%。

关键词：磷钼酸 HKUST 乙二醇单叔丁基醚异丁烯选择性醚化

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Synthesis and biological evaluation of 1,3,4-thiadiazole derivatives as type III secretion system inhibitors against Xanthomonas oryzae

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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 2019年 160卷 87-94页

作者： Tao, Hui Tian, Hao Jiang, Shan Xiang, xuwen Lin, Yinuo Ahmed, Wasim tang, Riyuan Cui, Zi-Ning South China Agr Univ Guangdong Prov Key Lab Microbial Signals & Dis Co Integrat Microbiol Res Ctr State Key Lab Conservat & Utilizat Subtrop Agrobi Guangzhou 510642 Guangdong Peoples R China South China Agr Univ Coll Mat & Energy Dept Appl Chem Guangzhou 510642 Guangdong Peoples R China

Xanthomonas oryzae pv. oryzae (Xoo) infection directly leads to a severe disease known as leaf blight, which is a major cause of yield loss of rice. Use of traditional bactericides has resulted in severe resistance in pathogenic bacteria. A new approach screening compounds that target the virulence factors rather than killing bacterial pathogens is imperative. In gram-negative bacteria, the type III secretion system (T3SS) is a conserved and significant virulence factor considered as a target for drug development. Therefore, we designed and synthesized a new series of 5-phenyl-2-furan carboxylic acid derivatives stitched with 2-mercapto-1,3,4-thiadiazole. Bioassays revealed that the title candidates attenuated the hypersensitive response through suppressing the promoter activity of a harpin gene hpa1 without affecting bacterial growth. Quantitative real time polymerase chain reaction (qRT-PCR) analysis demonstrated reduced the expression of several genes associated with T3SS, when title compounds were applied. Additionally, hrp gene cluster members, including hrpG and hrpX, had reduced mRNA levels. In vivo greenhouse tests showed that candidate compounds could alleviate the effects of Xoo infection in rice (Oryza sativa) and possess better protective activity against rice bacterial leaf blight than bismerthiazol and thiodiazole copper. All tested compounds were safe to rice. This work suggests there are new safe options for Xoo control in rice from these 1,3,4-thiadiazole derivatives.

关键词： 2-mercapto-1,3,4-thiadiazole Inhibitors Type III secretion system (T3SS) Anti-virulence factors Xanthomonas oryzae pv. oryzae (Xoo)

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Serum metabolomics reveals the intervention mechanism and compatible regularity of Chaihu Shu Gan San on chronic unpredictable mild stress-induced depression rat model

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JOURNAL OF PHARMACY AND PHARMACOLOGY 2020年第8期72卷 1133-1143页

作者： Zhang, Hongye Huang, Huimin Song, Hui Chin, Bonnie Zheng, Hua Ruan, Junxiang Wu, Fang Cheng, Bang Wu, Jinxia Liu, xuwen Liang, Yonghong Song, Fangming Chen, Zhaoni tang, Chaoling Lu, Shiyin Guo, Hongwei Zou, Zhongmei Su, Zhiheng Guangxi Med Univ Pharmaceut Coll Nanning 530021 Peoples R China Shenyang Pharmaceut Univ Dept Clin Pharm Shenyang Peoples R China Guangxi Med Univ Med Sci Res Ctr Nanning Peoples R China Guangxi Med Univ Chinese Minist Educ Key Lab Longev & Aging Related Dis Nanning Peoples R China Guangxi Med Univ Ctr Translat Med Nanning Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Inst Med Plant Dev Beijing 100193 Peoples R China

Objectives To provide a comprehensive study of the intervention mechanism and compatible regularity of Chaihu Shu Gan San (CSGS) in a chronic unpredictable mild stress (CUMS)-induced depression model. Methods Ethological study and ELISA assay were applied to measure the phenotypes of depression after CUMS stimulate and assess the antidepressant activity of fluoxetine, CSGS and its compatibilities. The serum metabolic profile changes were revealed by untargeted Q/TOF MS-based metabolomics followed by multivariate statistical analysis. Key findings CSGS exhibits an significant intervention effect on CUMS-induced depression. After the multivariate statistical analysis, 17 potential serum biomarkers were identified and 16 of them could be regulated by CSGS. The intervention of CSGS on CUMS-induced depression involved five key pathways. Moreover, each functional unit (monarch, minister, assistant and guide medicine) in CSGS regulates different metabolites and metabolic pathways to achieve different effects on antidepressant;however, their intervention efficacies are inferior to the holistic formula, which may be due to the synergism of bioactive ingredients in the seven herbs of CSGS. Conclusions CSGS produced an obvious antidepressant activity. The comprehensive and holistic metabolomics approach could be a powerful tool to study the intervention mechanism and the compatibility rule of traditional Chinese medicine.

关键词： antidepression Chaihu Shu Gan San chronic unpredictable mild stress-induced depression compatibility serum metabolomics ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry

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活血通降方对反流性食管炎大鼠食管损伤和xCT/VEGF-B/GPX4信号通路的影响

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中国中医药信息杂志 2022年第12期29卷 87-92页

作者：刘琰唐艳萍李培彩刘磊刘茜杨磊郝旭雯天津中医药大学研究生院天津301617 天津市南开医院天津300100

目的观察活血通降方对反流性食管炎(RE)大鼠食管损伤的影响,从xCT/VEGF-B/GPX4铁死亡信号通路探讨其作用机制。方法采用改良部分贲门肌切开术+外置幽门部分结扎术制备RE大鼠模型。将成模大鼠随机分为模型组及活血通降方低、高剂量组,每... 详细信息

目的观察活血通降方对反流性食管炎(RE)大鼠食管损伤的影响,从xCT/VEGF-B/GPX4铁死亡信号通路探讨其作用机制。方法采用改良部分贲门肌切开术+外置幽门部分结扎术制备RE大鼠模型。将成模大鼠随机分为模型组及活血通降方低、高剂量组,每组12只,另取12只作为空白组。造模成功当日开始给药,活血通降方低、高剂量组给药剂量分别为3.68、7.35 g/(kg·d),每日早晚各1次,连续灌胃14 d,空白组和模型组予5 mL生理盐水灌胃。观察大鼠食管黏膜损伤情况,HE染色观察黏膜组织病理变化,试剂盒检测食管组织丙二醛(MDA)、谷胱甘肽(GSH)、活性氧(ROS)含量,Western blot检测食管组织胱氨酸/谷氨酸反向转运体(xCT)、血管内皮生长因子B(VEGF-B)、谷胱甘肽过氧化物酶4(GPX4)蛋白表达,ELISA检测血清白细胞介素(IL)-1β、IL-17、IL-10含量。结果与空白组比较,模型组大鼠食管黏膜损伤明显,肉眼及病理评分显著升高,食管组织MDA、ROS含量增加,GSH含量减少,xCT、VEGF-B、GPX4蛋白表达降低,血清IL-1β、IL-17含量增加,IL-10含量减少,差异均有统计学意义(P<0.05,P<0.01);与模型组比较,活血通降方低、高剂量组大鼠食管黏膜损伤改善,肉眼及病理评分显著降低,食管组织MDA、ROS含量减少,xCT、VEGF-B、GPX4蛋白表达升高,血清IL-1β、IL-17含量减少,IL-10含量增加,活血通降方高剂量组大鼠食管组织GSH含量增加,差异均有统计学意义(P<0.05,P<0.01)。结论活血通降方可缓解RE大鼠食管黏膜炎症,修复食管损伤,其机制可能与激活xCT/VEGF-B/GPX4信号通路,增加其抗氧化性,提高抗氧化因子GSH水平,清除食管中过多的ROS,调节相关炎症因子水平,影响食管细胞铁死亡有关。

关键词：活血通降方反流性食管炎 xCT/VEGF-B/GPX4信号通路铁死亡食管损伤

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Paecilomyces variotii extracts (ZNC) enhance plant immunity and promote plant growth

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PLANT AND SOIL 2019年第1-2期441卷 383-397页

作者： Lu, Chongchong Liu, Haifeng Jiang, Depeng Wang, Lulu Jiang, Yanke tang, Shuya Hou, xuwen Han, Xinyi Liu, Zhiguang Zhang, Min Chu, Zhaohui Ding, Xinhua Shandong Agr Univ State Key Lab Crop Biol Shandong Prov Key Lab Biol Vegetable Dis & Insect Tai An 271018 Shandong Peoples R China Shandong Pengbo Biotechnol Co LTD Tai An 271018 Shandong Peoples R China Shandong Agr Univ Natl Engn Lab Efficient Utilizat Soil & Fertilize Coll Recourses & Environm Tai An 271018 Shandong Peoples R China

Background and aims The crude extract of the endophyte Paecilomyces variotii known as ZhiNengCong (ZNC) has function of promoting plant growth and enhancing disease resistance and is widely used in China. Our study aims to evaluate the molecular mechanisms of plant growth promotion and disease protection. Methods We generated transcriptome profiles from ZNC-treated seedlings using RNA sequencing. The function of salicylic acid (SA) in ZNC-mediated immunity was examined using SA biosynthesis and signaling pathway mutants. The concentrations of nitrogen (N) and phosphorus (P) in seedlings under ZNC treatment were measured. The effect of ZNC on the level of the hormone auxin in roots was tested using transgenic plants containing DR5::GFP. Results ZNC exhibited ultrahigh activity in promoting plant growth and enhancing disease resistance, even at concentrations as low as 1-10 ng/ml. ZNC induced ROS accumulation, callose deposition, and expression of PR genes. SA biosynthesis and signaling pathways were required for the ZNC-mediated defense response. Moreover, in improving plant growth, ZNC increased the level of auxin in root tips and regulated the absorption of N and P. Conclusion According to these results, ZNC is a highly effective plant elicitor that promotes plant growth by inducing auxin accumulation at the root tip at low concentrations and enhances plant disease resistance by activating the SA signaling pathway at high concentrations.

关键词： Plant elicitors Plant growth promotion Plant endophyte Disease resistance Auxin Salicylic acid

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