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Therapeutic Effects of Valeriana jatamansi on Ulcerative Colitis: Insights into Mechanisms of Action through Metabolomics and Microbiome Analysis

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JOURNAL OF PROTEOME RESEARCH 2023年第8期22卷 2669-2682页

作者： Wang, Lixia Zhang, Hai Tang, Fei Yan, Hongling Feng, wuwen Liu, Juan Wang, Yulan Tan, Yuzhu Chen, Hulan Chengdu Univ Tradit Chinese Med Pharm Coll Key Lab Southwestern Chinese Med Resources Chengdu 611137 Peoples R China Nanyang Technol Univ Singapore Phenome Ctr Lee Kong Chian Sch Med Singapore 639798 Singapore

Ulcerativecolitis (UC), belonging to inflammatory boweldisease(IBD), is a chronic and relapsing inflammatory disorder of the gastrointestinaltract, which has not been completely cured in patients so far. Valeriana jatamansi is a Chinese medicine used clinicallyto treat "diarrhea," which is closely related to *** study was to elucidate the therapeutic effects of V. jatamansi extract (VJE) on dextran sodium sulfate(DSS)-induced UC in mice and its underlying mechanism. In this work,VJE effectively ameliorates the symptoms and histopathological scoresand reduces the production of inflammatory factors in UC mice. Thecolon untargeted metabolomics analysis and 16S rDNA sequencing showedremarkable differences in colon metabolite profiles and intestinalmicrobiome composition between the control and DSS groups, and VJEintervention can reduce these differences. Thirty-two biomarkers werefound and modulated the primary pathways including pyrimidine metabolism,arginine biosynthesis, and glutathione metabolism. Meanwhile, twelvesignificant taxa of gut microbiota were found. Moreover, there isa close relationship between endogenous metabolites and intestinalflora. These findings suggested that VJE ameliorates UC by inhibitinginflammatory factors, recovering intestinal maladjustment, and regulatingthe interaction between intestinal microbiota and host ***, the intervention of V. jatamansi is a potential therapeutic treatment for UC.

关键词： Valeriana jatamansi ulcerative colitis metabolites gut microbiota correlation analysis

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Oxidation behavior and atomic structural transition of size-selected coalescence-resistant tantalum nanoclusters

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NANOTECHNOLOGY 2024年第31期35卷 315603-315603页

作者： Hu, Shengyong Hu, Kuojuei Zhang, Yongxin Shah, Syed Adil Zhao, Zixiang Zuo, Zewen Lu, Siqi Tang, Sichen Zhu, wuwen Fang, Liu Song, Fengqi Nanjing Univ Collaborat Innovat Ctr Adv Microstruct Sch Phys Natl Lab Solid State Microstruct Nanjing 210093 Peoples R China Nanjing Univ Inst Atom Mfg Suzhou Campus Nanjing 215163 Peoples R China Shenzhen Univ Sch Biomed Engn Hlth Sci Ctr Shenzhen 518060 Guangdong Peoples R China

Herein a series of size-selected Ta-N (N = 147, 309, 561, 923, 1415, 2057, 6525, 10 000, 20 000) clusters are generated using a gas-phase condensation cluster beam source equipped with a lateral time-of-flight mass-selector. Aberration-corrected scanning transmission electron microscopy (AC-STEM) imaging reveals good thermal stability of Ta N clusters in this study. The oxidation-induced amorphization is observed from AC-STEM imaging and further demonstrated through x-ray photoelectron spectroscopy and energy-dispersive spectroscopy. The oxidized Ta predominantly exists in the +5 oxidation state and the maximum spontaneous oxidation depth of the Ta cluster is observed to be 5 nm under prolonged atmosphere exposure. Furthermore, the size-dependent sintering and crystallization processes of oxidized Ta N clusters are observed with an in situ heating technique, and eventually, ordered structures are restored. As the temperature reaches 1300 degrees C, a fraction of oxidized Ta309 clusters exhibit decahedral and icosahedral structures. However, the five-fold symmetry structures are absent in larger clusters, instead, these clusters exhibit ordered structures resembling those of the crystalline Ta2O5 films. Notably, the sintering and crystallization process occurs at temperatures significantly lower than the melting point of Ta and Ta2O5, and the ordered structures resulting from annealing remain well-preserved after six months of exposure to ambient conditions.

关键词： size-selected Ta- N clusters oxidation thermal stability amorphization sintering crystallization

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Coalescence behavior of size-selected gold and tantalum nanoclusters under electron beam irradiation: insights into nano-welding mechanisms

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NANOSCALE ADVANCES 2024年第16期6卷 4237-4246页

作者： Hu, Shengyong Shah, Syed Adil Shah, Syed Niaz Ali Zhao, Zixiang Zhu, wuwen Zhang, Yongxin Lu, Siqi Tang, Sichen Hu, Kuo-Juei Song, Fengqi Nanjing Univ Collaborat Innovat Ctr Adv Microstruct Sch Phys Natl Lab Solid State Microstruct Nanjing 210093 Peoples R China Nanjing Univ Inst Atom Mfg Suzhou Campus Nanjing 215163 Peoples R China Shenzhen Univ Hlth Sci Ctr Sch Biomed Engn Shenzhen 518060 Guangdong Peoples R China King Fahd Univ Petr & Minerals Innovat & Technol Transfer Dhahran 31261 Saudi Arabia

The emerging technique of nano-welding (NW) via precisely regulating the fusion of nanoclusters (NCs) in nanotechnology has attracted significant attention for its innovative approach. Employing the gas-phase condensation cluster source with a lateral time-of-flight (TOF) mass-selector, size-selected gold (Au), and tantalum (Ta) NCs were prepared. This study explores the coalescence behavior of size-selected Au and Ta NCs under electron beam irradiation, aiming to investigate the related mechanism governing the welding process. Intrinsically driven by the reduction of excess surface energy, electron beam induces atomic thermal migration, fostering sintering neck growth at cluster interfaces. During this process, atomic diffusion and recrystallization enable NCs to alter shape while retaining stable facet planes. Aberration-corrected scanning transmission electron microscopy (AC-STEM) showcases the formation of single or polycrystalline sintered clusters, during which some lattice distortions can be eliminated. Interestingly, oxidized Ta clusters experience knock-on damage caused by elastic scattering of electron beams, partially deoxidizing them. Additionally, electron-phonon inelastic scattering transforms oxidized Ta clusters from amorphous to crystalline structures. Moreover, the quantum size effect and surface effect of NCs facilitate the surpassing of miscibility limits during Au-Ta heterogeneous welding processes. This investigation bridges the gap between fundamental research on cluster materials and their practical applications. The emerging technique of nano-welding (NW) via precisely regulating the fusion of nanoclusters (NCs) in nanotechnology has attracted significant attention for its innovative approach.

关键词： Electrons

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Runx1 Deficiency Promotes M2 Macrophage Polarization Through Enhancing STAT6 Phosphorylation

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INFLAMMATION 2023年第6期46卷 2241-2253页

作者： Zhou, Siyuan Zhao, Ting Chen, Xuqiong Zhang, wuwen Zou, Xiaoyi Yang, Yi Wang, Qinshi Zhang, Ping Zhou, Tong Feng, Tongbao Nanjing Med Univ Affiliated Changzhou Peoples Hosp 2 Dept Clin Lab Changzhou 213000 Jiangsu Peoples R China Shanghai Jiao Tong Univ Ruijin Hosp Sch Med Dept Pediat Shanghai 200025 Peoples R China

Our previous study had demonstrated that Runx1 promoted LPS-induced macrophage inflammatory response, however, the role of Runx1 in M2 macrophage polarization still remains largely unknown. This study was conducted to investigate the role of Runx1 in IL-4/IL-13-induced M2 macrophage polarization and its potential regulatory mechanism. We found that exposure of macrophages to IL-4/IL-13 induced a remarkable increasement in Runx1 expression level. Specifically, we established genetically modified mice lacking Runx1 in myeloid cells, including macrophages. RNA-Seq was performed to identify differentially expressed genes (DEGs) between Runx1 knockout and WT control bone marrow-derived macrophages (BMDMs). We identified 686 DEGs, including many genes which were highly expressed in M2 macrophage. In addition, bioinformatics analysis indicated that these DEGs were significantly enriched in extracellular matrix-related processes. Moreover, RT-qPCR analysis showed that there was an obvious upregulation in the relative expression levels of M2 marker genes, including Arg1, Ym1, Fizz1, CD71, Mmp9, and Tgm2, in Runx1 knockout macrophages, as compared to WT controls. Consistently, similar results were obtained in the protein and enzymatic activity levels of Arg1. Finally, we found that the STAT6 phosphorylation level was significantly enhanced in Runx1 knockout macrophages, and the STAT6 inhibitor AS1517499 partly reduced the upregulated effect of Runx1 deficiency on the M2 macrophage polarization. Taken together, Runx1 deficiency facilitates IL-4/IL-13-induced M2 macrophage polarization through enhancing STAT6 phosphorylation.

关键词： Runx1 M2 macrophage STAT6 bioinformatics analysis

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Essential role of ascO for virulence of Aeromonas veronii and inducing apoptosis

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JOURNAL OF FISH DISEASES 2022年第10期45卷 1477-1489页

作者： Zhao, Tong Yang, Bintong Li, Hongjin Qian, Aidong Cong, Wei Sun, wuwen Kang, Yuanhuan Jilin Agr Univ Coll Anim Sci & Technol Coll Vet Med Changchun 130118 Peoples R China Shandong Univ Marine Coll Weihai Peoples R China

Aeromonas veronii is a significant pathogen that is capable of infecting humans, animals, and aquatic animals. The type III secretion system (T3SS) is intimately associated with bacterial pathogenicity. The ascO gene is an important core component of T3SS in A. veronii, but its function is still unclear. The ascO gene of A. veronii TH0426 was deleted by using the pRE112 suicide plasmid to study its function. The study results showed that the ability of increment ascO to adhere and invade EPC cells was significantly reduced by 1.28 times. The toxicity of the mutant strain Delta ascO to EPC cells was consistently significantly lower than wild-type strain TH0426 at 1, 2, and 4 h. The LD50 values of increment ascO against zebrafish and Carassius auratus (C. auratus) were 53 and 15 times that of the wild-type strain. In addition, the bacterial load of the mutant strain Delta ascO in blood, heart, liver, and spleen was lower than wild-type strain TH0426. The Hoechst staining showed that the apoptotic degree of EPC cells induced by the mutant strain Delta ascO was lower than that of the wild-type strain TH0426. Furthermore, real-time quantitative PCR (RT-qPCR) analysis revealed lower expression levels of pro-apoptotic genes (including cytC, cas3, cas9, TNF-alpha, and IL-1 beta) in C. auratus tissues infected with the mutant strain Delta ascO compared to the wild-type strain TH0426. The results of in vivo and in vitro experiments have shown that ascO gene mutation can reduce the adhesion and toxicity of A. veronii to EPC and reduce the level of apoptosis induced by A. veronii. As a result, these insights will help further elucidate the function of the ascO gene and thus contribute to understanding the pathogenesis of A. veronii.

关键词： Aeromonas veronii ascO cell apoptosis virulence

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The role of protein arginine methyltransferase 7 in human developmentally arrested embryos cultured in vitro

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生物化学与生物物理学报（英文版） 2021年第7期53卷 925-932页

作者： wuwen Zhang Shifeng Li Kai Li Li Li Ping Yin Guoqing Tong Reproductive Medicine Center Shuguang Hospital affiliated to Shanghai University of Traditional Chinese MedicineShanghai 201203China Center for Excellence in Molecular Cell Science Chinese Academy of SciencesShanghai 200031China

Human embryos of in vitro fertilization (IVF) are often susceptible to developmental arrest,which greatly reduces the efficiency of IVF *** recent years,it has been found that protein argi-nine methyltransferase 7 (PRMT7) plays an important role in the process of early embryonic ***,not much is known about the relationship between PRMT7 and developmentally arrested *** role of PRMT7 in developmentally arrested embryos was thus investigated in this *** human embryos from IVF were collected for experimental *** real-time polymerase chain reaction (qRT-PCR) and confocal analyses were used to identify PRMT7 mRNA and protein levels in early embryos at different developmental stages,as well as changes in the methylation levels of ***,PRMT7 was knocked down in the developmentally arrested embryos to observe the further development of these *** results demonstrated that PRMT7 mRNA and protein levels in arrested embryos were significantly increased compared with those in control embryos;meanwhile,the methylation levels of H4R3me2s in arrested embryos were also increased *** of PRMT7 could rescue partially developmentally arrested embryos,and even individual developmentally arrested embryos could develop into *** conclusion,over-expression of PRMT7 dis-rupts the early embryo development process,leading to early embryos developmental arrest,but these developmentally arrested defects could be partially rescued by knockdown of the PRMr7 protein.

关键词： human embryo embryonic development developmental arrest protein arginine methyltransferase 7 in vitro fertilization

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Inhibiting TGF-β 1-Mediated Cellular Processes as an Effective Strategy for the Treatment of Pulmonary Fibrosis with Chinese Herbal Medicines

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AMERICAN JOURNAL OF CHINESE MEDICINE 2021年第8期49卷 1965-1999页

作者： Wang, Jing Zhao, Xingtao Feng, wuwen Li, Yunxia Peng, Cheng Chengdu Univ Tradit Chinese Med Sch Pharm State Key Lab Characterist Chinese Med Resources Chengdu 611137 Peoples R China

Pulmonary fibrosis (PF) is a chronic and irreversible interstitial lung disease that even threatens the lives of some patients infected with COVID-19. PF is a multicellular pathological process, including the initial injuries of epithelial cells, recruitment of inflammatory cells, epithelial-mesenchymal transition, activation and differentiation of fibroblasts, etc. TGF-beta 1 acts as a key effect factor that participates in these cellular processes of PF. Recently, much attention was paid to inhibiting TGF-beta 1 mediated cell processes in the treatment of PF with Chinese herbal medicines (CHM), an important part of traditional Chinese medicine. Here, this review first summarized the effects of TGF-beta 1 in different cellular processes of PF. Then, this review summarized the recent research on CHM (compounds, multi-components, single medicines and prescriptions) to directly and/or indirectly inhibit TGF-beta 1 signaling (TLRs, PPARs, micrRNA, etc.) in PF. Most of the research focused on CHM natural compounds, including but not limited to alkaloids, flavonoids, phenols and terpenes. After review, the research perspectives of CHM on TGF-beta 1 inhibition in PF were further discussed. This review hopes that revealing the inhibiting effects of CHM on TGF-beta 1-mediated cellular processes of PF can promote CHM to be better understood and utilized, thus transforming the therapeutic activities of CHM into practice.

关键词： Pulmonary Fibrosis TGF-beta 1 Cellular Processes Traditional Chinese Medicine Review

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基于大语言模型的人文社科类业务智能体构建平台研究

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情报资料工作 2024年第5期45卷 82-91页

作者：刘耀周家辉邱武文中国科学技术信息研究所北京100038 北京大学软件与微电子学院北京102600

[目的/意义]为向人文社科领域研究人员提供业务文本分析与业务模型构建服务,搭建业务智能体构建平台以提供高效支持。[方法/过程]文章以“大模型+智能体”为指导思想,提出大语言模型自动迭代微调机制,基于业务流程认知图谱生成、资源解... 详细信息

[目的/意义]为向人文社科领域研究人员提供业务文本分析与业务模型构建服务,搭建业务智能体构建平台以提供高效支持。[方法/过程]文章以“大模型+智能体”为指导思想,提出大语言模型自动迭代微调机制,基于业务流程认知图谱生成、资源解析工具按需生成、低代码业务功能开发等技术,通过PaaS模式搭建面向人文社科领域的业务智能体构建平台。[结果/结论]平台具备业务资源定制化生成、业务模型自动构建与可视化能力,利用平台构建业务智能体能够进行具体业务分析,为人文社科领域研究工作提供支持。

关键词：大语言模型智能体平台即服务业务模型

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Wuzi Yanzong Pill alleviates spermatogenesis dysfunction by modulating the gut microbial tryptophan metabolites

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ARABIAN JOURNAL OF CHEMISTRY 2024年第7期17卷

作者： Liu, Juan Feng, wuwen Zhang, Dandan Cheng, Hao Zhou, Yaochuan Wu, Jing Wang, Zixuan Wang, Zhilei Fang, Chunyan Li, Guangsen You, Yaodong Yu, Xujun Chang, Degui Hosp Chengdu Univ Tradit Chinese Med TCM Regulating Metab Dis Key Lab Sichuan Prov Chengdu 610032 Peoples R China Chengdu Univ Tradit Chinese Med Sch Pharm State Key Lab Southwestern Chinese Med Resources Chengdu 611137 Peoples R China Chengdu Univ Tradit Chinese Med Key Lab Minist Educ Standardizat Chinese Med Chengdu 611137 Peoples R China Shanghai Univ Tradit Chinese Med Inst Chinese Mat Med Shanghai Key Lab Complex Prescript Shanghai 201203 Peoples R China Shanghai Univ Tradit Chinese Med MOE Key Lab Standardizat Chinese Med Shanghai 201203 Peoples R China

Wuzi Yanzong Pill (WZYZP), a classic traditional Chinese medicine prescription, has been widely used to alleviate spermatogenesis dysfunction for hundreds of years. However, the molecular mechanisms of WZYZP treatment for spermatogenesis dysfunction remain limited. Here, our results showed that WZYZP significantly improved spermatogenesis and testicular energy metabolism. The 16S rDNA sequencing showed that WZYZP improved gut microbiota dysbiosis, including increased relative abundances of Lactobacillus with the capability of metabolizing Trp. The depletion of gut microbiota by antibiotics suppressed the ability of WZYZP to improve spermatogenesis dysfunction. The untargeted and targeted metabolomics results showed that WZYZP increased Trp metabolites especially aryl hydrocarbon receptor (AhR) ligands. Moreover, WZYZP modulated the AhR/ AMPK pathway to improve the expression of spermatogenesis-related genes. The correlation analysis showed that gut microbiota was significantly correlated with Trp metabolites, and Trp metabolites were closely related to spermatogenesis. Overall, the results demonstrated that WZYZP could regulate gut microbiota, and the gut microbial Trp metabolites further act on testicular energy metabolism, thereby indirectly improving spermatogenic dysfunction. Our study provides a novel research strategy for complementary and alternative medicine in male infertility from the perspective of gut microbial metabolites.

关键词： Wuzi Yanzong Pill Spermatogenesis dysfunction Gut microbiota Tryptophan metabolites Energy metabolism

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Superlattice assembly strategy of small noble metal nanoparticles for surface-enhanced Raman scattering

COMMUNICATIONS MATERIALS

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COMMUNICATIONS MATERIALS 2024年第1期5卷 65页

作者： Yao, Chang Yan, wuwen Dong, Ronglu Dou, Shaobin Yang, Liangbao Univ Sci & Technol China Hefei 230026 Anhui Peoples R China Chinese Acad Sci Hefei Inst Phys Sci Inst Hlth & Med Technol Hefei 230031 Peoples R China Chinese Acad Sci Hefei Canc Hosp Dept Pharm Hefei 230031 Anhui Peoples R China Anhui Cas Art Technol Co Ltd Hefei 230088 Peoples R China

The self-assembly of noble metal nanoparticles into periodic structures has been a theme of great interest for surface-enhanced Raman scattering (SERS) and use in functional devices. However, small nanoparticle self-assembly faces numerous challenges in tunability, referring to controlling their structural properties like structure, gaps, and arrangement. These issues highlight the need for further research and development to enhance the tunability and stability of self-assembled small nanoparticles. Here, we report a general centimeter-scale superlattice assembly strategy for noble metal nanoparticles less than 15 nm in size. Not only is this monolayer superlattice assembly generally applicable to different kinds and sizes of noble metal nanoparticles, but also, the crystal plane spacing can also be quickly and conveniently controlled by changing the ethanol concentration. SERS results reveal that optimized superlattice membranes of noble metal nanoparticles possess high detection sensitivity and ordered hot spots. Therefore, our strategy offers prospects for high-performance SERS substrates based on small noble metal nanoparticle superlattices. Self-assembly of small nanoparticles is difficult to control and the resultant structures have weak stability. Here, a general centimeter-scale superlattice assembly strategy for noble metal nanoparticles of less than 15 nm is used to yield stable hexagonal close-packed monolayers.

关键词： Substrates

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