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Biological analysis of phage vB_AhaP_PT2 and treatment rescued crucian carp infected with Aeromonas hydrophila

AQUACULTURE 2025年第Part2期595卷

作者： Liang, Shuang Liang, Ruiqi Raza, Sayed Haidar Abbas Huang, Qixing Li, Tingxuan Bai, Huifang Feng, Chao Xin, Liang Guo, Hui Wang, Shun Yu, Jiahao Cao, Yuan Liu, Ning Almalki, Fatemah Zhang, Dongxing Sun, wuwen Zhang, Lei Jilin Agr Univ Coll Anim Sci & Technol Changchun 130000 Peoples R China Changchun Borui Technol Co Ltd Changchun 130000 Peoples R China Yangzhou Univ Coll Vet Yangzhou 225009 Peoples R China South China Agr Univ Guangdong Prov Key Lab Food Qual & Safety Guangzhou 510642 Peoples R China Jilin Lanpu Haoye Technol Co Changchun 130000 Peoples R China Northeast Agr Univ Coll Vet Med Harbin 150030 Peoples R China Shaqra Univ Coll Sci & Humanities Al Quwaiiyah Biol Dept Al Quwaiiyah 19257 Saudi Arabia

Phage therapy is an alternative approach to overcoming the problem of multidrug resistance in bacteria. In this study, we isolated and purified a virulent phage named vB_AhaP_PT2 from A. hydrophila-TPS. We examined the biological characteristics and genome sequences of vB_AhaP_PT2. After intraperitoneal injection of bacteriophage (8 x 107 PFU/mL) into crucian carp, most of the phage distributed in the liver, spleen, kidney, intestine, and peripheral blood, and disappeared within 72 h. No substantial adverse symptoms were found in major organs after receiving phage injections continuously for 14 days. Vitro and Vivo results indicated that phage can effectively eliminate A. hydrophila-TPS. The phage vB_AhaP_PT2 (8 x 107 PFU/mL, 100 mu L) can effectively protect the fish that exhibited a double median lethal dose of bacteria (2 x 106 CFU/fish). Although phage vB_AhaP_PT2 triggered the generation of phage-specific antibodies in carp, when the antibody titer was maximum, 8 x 107 PFU doses of bacteriophage could still protect crucian carp with lethal bacterial infections, the survival rate could reach 80%. We also found that members of the genus Aeromonas could enter and colonize the intestine. The phage vB_AhaP_PT2 reduced the number of colonies of the genus Aeromonas. However, no significant changes were observed in alpha-diversity and beta-diversity parameters, which suggested that the consumed phage had little effect on the intestine microflora. All the above results suggested that vB_AhaP_PT2 was a safe and effective therapeutic that can be considered as a potential tool to treat the infection caused by A. hydrophilaTPS in aquaculture.

关键词： Aeromonas hydrophila Bacteriophage therapy Distribution of phage Antibody

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Inversion method of hydraulic conductivity for steady-state problem based on reduced-order model constructed by improved greedy sampling method

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ADVANCES IN WATER RESOURCES 2022年 166卷

作者： Qian, wuwen Chai, Junrui Zhao, Xinyu Niu, JingTai Xiao, Fang Deng, Zhiping Nanchang Inst Technol Coll Water Conservancy & Ecol Engn Nanchang 330099 Peoples R China Xian Univ Technol State Key Lab Ecohydraul Northwest Arid Reg China Xian 710048 Peoples R China

To solve the time-consuming parameter inversion problem based on swarm evolution algorithm, this study proposes an improved greedy sampling method (GSM) (IGSM) based on model reduction technology to rapidly estimate the hydraulic conductivity of steady-state seepage problem. Different from the traditional GSM, IGSM neither needs to estimate the posterior error bound of the reduced-order model (ROM) nor does it require a training sample set to train the ROM. Instead, the observation data are directly applied to the training process of ROM. Each iterative process of IGSM includes a parameter inversion process to obtain the parameters that best match the observation information and use it to update the current ROM. The improved differential evolution algorithm (e.g., MMRDE) is used as the search algorithm, and the resulting algorithm is denoted as IGSMMMRDE. After comparative testing, IGSM-MMRDE was found to have higher accuracy and lower calculation cost than GSM-MMRDE. In addition, IGSM-MMRDE is also capable of high-dimensional parameter inversion problems and parameter inversion with a wider search range. After applying IGSM-MMRDE to the inversion of the rock mass hydraulic conductivity of the natural seepage field in the dam site area of a hydropower station, IGSM-MMRDE converges quickly and takes much less time than ADINA-MMRDE based on the full-order model (approximately 0.31% of ADINA-MMRDE). The performance results are even better than the ADINA-MMRDE using the fixed evolutionary generation. Therefore, the advantages of using IGSM in the rapid inversion of the initial seepage field of large-scale projects are extremely evident.

关键词： Reduced-order Greedy snapshot Hydraulic conductivity Inversion method Steady-state seepage

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Natural products: Harnessing the power of gut microbiota for neurological health

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PHYTOMEDICINE 2024年 135卷 156019页

作者： Cheng, Hao Liu, Juan Zhang, Dandan Wu, Jing Wu, Jinlu Zhou, Yaochuan Tan, Yuzhu Feng, wuwen Peng, Cheng Chengdu Univ Tradit Chinese Med Sch Pharm State Key Lab Southwestern Chinese Med Resources Chengdu Peoples R China Hosp Chengdu Univ Tradit Chinese Med TCM Regulating Metab Dis Key Lab Sichuan Prov Chengdu 610072 Peoples R China Chengdu Univ Tradit Chinese Med Sch Basic Med Sci Chengdu 611137 Peoples R China

Background: Neurological diseases are the primary cause of disability and death and impose substantial financial burdens. However, existing treatments only relieve symptoms and may cause many adverse effects. Natural products are a promising source of neurological therapeutic agents due to their excellent neuroprotective effect and safety. The gut microbiota has an essential impact on maintaining brain homeostasis via the gut-brain axis. Multiple investigations show that natural products offer neuroprotective effects by regulating gut microbiotadriven signaling networks. Objectives: This review aims to provide a systematic review of how natural products promote neurological health by harnessing the power of gut microbiota. Methods: The pre-January 1, 2024 literature was gathered from several databases, including Scopus, PubMed, Google Scholar, and Web of Science, utilizing appropriate keywords. The gathered publications underwent a review process and were classified based on their study content, specifically focusing on the impact of natural products on gut microbiota and neurological health. Results: Here, we review how natural products promote neurological health by regulating the gut microbiotabrain axis. Specifically, we focus on the following areas. (1) Altering microorganism community structure, including increasing alpha-diversity and altering (1-diversity. (2) Regulating the population of certain bacteria, including enriching beneficial microorganisms Akkermansia and Bifidobacterium, , and inhibiting potentially hazardous microorganisms Bilophila, Klebsiella, , and Helicobacter. . (3) Regulating microbial neuroactive metabolites levels, including short-chain fatty acids, tryptophan and its derivatives, trimethylamine N-oxide, dopa/ dopamine, gamma-aminobutyric acid, and lipopolysaccharide. Furthermore, we review how natural products promote neurological health by regulating intestinal barrier homeostasis. Conclusion: Natural products promote neurological hea

关键词： Natural products Gut microbiota Neurological diseases Neuroinflammation Short-chain fatty acids Lipopolysaccharide

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Intracage vibrations and Zeeman effect in Y3N@C80 single-molecule transistors

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APPLIED PHYSICS LETTERS 2024年第15期125卷

作者： Chen, Jun Zhu, wuwen Xi, Caigan Zhou, Yuanbiao Shui, Yuan Qi, Haoran Liu, Xinrong Mao, Yifu Zhang, Minhao Yang, Tao Tan, Yuanzhi Nanjing Univ Collaborat Innovat Ctr Adv Microstruct Natl Lab Solid State Microstruct Nanjing 210093 Peoples R China Nanjing Univ Sch Phys Nanjing 210093 Peoples R China Nanjing Univ Inst Atom Mfg Suzhou 215163 Peoples R China Xiamen Univ Coll Chem & Chem Engn State Key Lab Phys Chem Solid Surfaces Xiamen 361005 Peoples R China Xi An Jiao Tong Univ Sch Phys MOE Key Lab Nonequilibrium Synth & Modulat Condens Xian 710049 Peoples R China

Clusterfullerenes, which contain a cluster rather than single-atom inclusions, exhibit more complex internal structures and greater degrees of freedom for motion. Trimetallic nitride clusterfullerenes have attracted significant attention due to their diversity and potential applications, among which Y3N@C-80 stands out for its charge-transfer characteristics in electronic excitations, owing to the unique distribution of molecular orbitals near the Fermi level. Here, we have fabricated single-molecule transistor devices using Y3N@C-80. Transport measurements at liquid helium temperature revealed a series of excited state energy levels, which were matched to corresponding vibrational modes through comparison with Raman spectra and density functional theory calculations. Additionally, we measured charge-state-dependent magnetic responses, revealing the electron and spin-filling patterns of the molecular orbitals in Y3N@C-80. These results enhance our understanding of the dynamics and molecular spin-orbit characteristics of clusterfullerenes, indicating their potential for multifunctional applications.

关键词： Molecular orbitals

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Study on Influencing Factors of Local Government Implicit Debt in PPP Project Based on Factor Analysis 2nd

Study on Influencing Factors of Local Government Implicit De...

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2nd International Conference on Decision Science and Management, ICDSM 2019

作者： Cao, wuwen Leng, Chao XiHua University No. 999 Jinzhou Road Jinniu District ChengduSichuan China

ISBN: (纸本)9789811535871

In the process of promoting PPP financing model for project construction in China, the scope of implicit debt of local government is not clearly defined, and the debt burden is too large because of the system, norms and professional cognition from the government perspective. Therefore, this paper analyzes the reasons for the formation of local government implicit debt in PPP projects, constructs an index system of influencing factors of local government implicit debt, introduces the factor analysis method to classify and analyze the influencing factors of the implicit debt of local government. © Springer Nature Singapore Pte Ltd. 2020.

关键词： Factor analysis

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Protective immune-response of Aeromonas hydrophila phage lysate in crucian carp against direct virulent challenge with A. hydrophila-TPS

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FISH & SHELLFISH IMMUNOLOGY 2024年 155卷 110011页

作者： Liang, Ruiqi Cui, Ziyin Raza, Sayed Haidar Abbas Li, Tingxuan Zhang, Zhe Huang, Qixing Bai, Huifang Cheng, Yan Du, Bingmei Li, Jinhong Zhu, Wenbin Ren, Xiaochen Cao, Yuan Liu, Ning Althagafi, Hind Jaber Fallatah, Deema Sun, wuwen Zhang, Lei Jilin Agr Univ Coll Anim Sci & Technol Coll Vet Med Changchun 130118 Peoples R China Liaoning Univ Tradit Chinese Med Coll Expt Anim Med Shenyang 116600 Peoples R China Borui Technol Co Ltd Changchun 130000 Peoples R China South China Agr Univ Guangdong Prov Key Lab Food Qual & Safety Guangzhou 510642 Peoples R China Jilin Lanpu Haoye Technol Co Changchun 130000 Peoples R China Northeast Agr Univ Coll Vet Med Harbin Heilongjiang Peoples R China Princess Nourah bint Abdulrahman Univ Coll Sci Dept Biol POB 84428 Riyadh 11671 Saudi Arabia Prince Sattam Bin Abdulaziz Univ Coll Appl Med Sci Dept Med Lab Sci Al Kharj 11942 Saudi Arabia

Aeromonas hydrophila was a common opportunistic pathogen that was widely found in various aquatic environment and could cause multiple infections in humans and animals. The haemorrhagic septicemia and bacterial enteritis in fish triggered by this pathogen led to significant losses in the aquaculture industry. In this study, we aimed to develop a phage lysate vaccine by lysing the A. hydrophila-TPS strain using phage PZY-Ah, which was previously isolated and preserved in the laboratory. First, we focused on optimizing phage lysis conditions, including different host bacteria culture time, phage lysis time, and phage to bacterial ratios. The optimal conditions were established as follows: culturing the A. hydrophila-TPS strain for 6 h, adding phage at a ratio of 1:10, and mixing for 4 h, which resulted in maximum lysis of the host bacteria. Subsequently, we assessed the immune responses of groups receiving formaldehyde-inactivated vaccines compared to those receiving various concentrations of the phage lysate vaccine. Both the TPS-phage lysate and formaldehyde-inactivated vaccine groups exhibited increased levels of specific immune enzymes (ACP, AKP, LZM, SOD, CAT) and cytokines (IL-1 beta, TNF-alpha and IFN-gamma) in serum, as well as enhanced humoral immunity (IgM, C3 and C4) in crucian carp. Furthermore, challenge tests conducted post-immunization demonstrated that the high concentration of the TPS strain-lysate vaccine group (1 x 108 CFU/mL) achieved the highest immune protection rate at 88.89 %. Overall, the development of the TPS-phage lysate vaccine significantly enhanced the immunity of crucian carp, providing a higher level of protection and establishing a foundation for the research and development of phage-based aquatic vaccines.

关键词： Phage lysate vaccine Aeromonas hydrophila Immune protection Crucian carp

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Single-cell transcriptome analysis of human oocyte ageing

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 2021年第13期25卷 6289-6303页

作者： Yuan, Lihua Yin, Ping Yan, Hua Zhong, Xiufang Ren, Chunxia Li, Kai Chin Heng, Boon Zhang, wuwen Tong, Guoqing Shanghai Univ Tradit Chinese Med Shuguang Clin Med Coll Shanghai Peoples R China Shanghai Univ Tradit Chinese Med Shuguang Hosp Ctr Reprod Med Shanghai 200120 Peoples R China Peking Univ Beijing Peoples R China

Oocyte ageing is a key bottleneck and intractable challenge for in vitro fertilization treatment of aged female patients. The underlying molecular mechanisms of human oocyte ageing remain to be elucidated. Hence, this study aims to investigate the key genes and relevant biological signalling pathways involved in human oocyte ageing. We isolated mRNA for single-cell RNA sequencing from MII human oocytes donated by patients undergoing intracytoplasmic sperm injection. Nine RNA-seq datasets were analyzed, which included 6 older patients(average 42.67 +/- 2.25 years) and 3 younger patients (average 25.67 +/- 2.08 years). 481 differentially expressed genes (DEGs) were identified, including 322 upregulated genes enriched in transcription, ubiquitination, epigenetic regulation, and cellular processes, and 159 downregulated genes enriched in ubiquitination, cell cycle, signalling pathway, and DNA repair. The STRING database was used to analyse protein-protein interactions, and the Cytoscape software was used to identify hub genes. From these DEGs, 17 hub genes were identified including 12 upregulated genes (UBE2C, UBC, CDC34, UBR1, KIF11, ASF1B, PRC1, ESPL1, GTSE1, EXO1, UBA1, KIF4A) and 5 downregulated genes (UBA52, UBE2V2, SKP1, CCNB1, MAD2L1). The significant key biological processes that are associated with these hub genes include ubiquitin-mediated proteolysis, ubiquitination-related pathways, oocyte meiosis, and cell cycle. Among these, UBE2C may play a crucial role in human oocyte ageing.

关键词： ageing biological information data mining gene expression human oocyte oocyte meiosis sc RNA‐ seq ubiquitination

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Density functional theory studies on combustion oxidative decomposition mechanism of 3,3,3-trifluoropropene

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COMPUTATIONAL AND THEORETICAL CHEMISTRY 2024年 1241卷

作者： Feng, Biao Chen, wuwen Wang, Jiachang Wu, Hongying Zhang, Zhao Jian, Linrui Li, Siqi Yang, Zhao Wang, Dan Ma, Suxia Taiyuan Univ Technol Coll Elect & Power Engn Key Lab Cleaner Intelligent Control Coal & Elect Minist Educ MOE Taiyuan 030024 Peoples R China Tianjin Univ State Key Lab Engines Tianjin 300350 Peoples R China Taiyuan Univ Technol Key Lab Adv Transducers & Intelligent Control Syst Minist Educ MOE Taiyuan 030024 Peoples R China

The environmentally friendly refrigerant 3,3,3-trifluoropropene (HFO-1243zf) suitable for refrigeration and heat pump systems has flammability. Based on density functional theory, the oxidation decomposition mechanism of 3,3,3-trifluoropropene was studied, including the main initial oxidation decomposition and subsequent reactions. The results indicate that among the initial thermal decomposition reactions, the reaction with the lowest energy barrier is simultaneous elimination of HF, followed by CC bond breaking to form center dot CF3. In the H abstraction reaction, the reaction energy barrier of H atom capture by center dot F is the lowest. In F abstraction reaction, the center dot H is the most active. In the addition reaction, the reaction energy barrier of center dot F is the lowest. The energy barriers of reactions involving different free radicals, from high to low, are: thermal decomposition, F abstraction, H abstraction and addition reaction. The subsequent reactions mainly involve the generation pathways of center dot CF3 and the formation of stable products.

关键词： Decomposition Computational chemistry Refrigerant 3,3,3-trifluoropropene

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CDC26 is a key factor in human oocyte aging

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HUMAN REPRODUCTION 2021年第12期36卷 3095-3107页

作者： Li, Li Xia, Ye Yang, Yang Zhang, wuwen Yan, Hua Yin, Ping Li, Kai Chen, Yuanyuan Lu, Lu Tong, Guoqing Shanghai Univ Tradit Chinese Med Reprod Med Ctr Shuguang Hosp 528 Zhangheng Rd Shanghai 201203 Peoples R China

STUDY QUESTION: Is CDC26 a key factor in human oocyte aging? SUMMARY ANSWER: The lack of CDC26 disrupts the oocytes maturation process, leading to oocyte aging, but these defects could be partially rescued by overexpression of the CDC26 protein. WHAT IS KNOWN ALREADY: Age-related oocyte aging is the main cause of female fertility decline. In mammalian oocytes, aberrant meiosis can cause chromosomal abnormalities that might lead to infertility and developmental disorders. CDC26 participates in the meiosis process. STUDY DESIGN, SIZE, DURATION: Differential gene expression in young and old women oocytes were screened by single-cell RNA-seq technology, and the functions of differentially genes were verified on mouse oocytes. Finally, transfection technology was used to evaluate the effect of a differentially expressed gene in rescuing human oocyte from aging. PARTICIPANTS/MATERIALS, SETTING, METHODS: Discarded human oocytes were collected for single-cell RNA-seq, q-PCR and immunocytochemical analyses to screen for and identify differential gene expression. Female KM mice oocytes were collected for IVM of oocytes, q-PCR and immunocytochemical analyses to delineate the relationships between oocyte aging and differential gene expression. Additionally, recombinant lentiviral vectors encoding CDC26 were transfected into the germinal vesicle oocytes of older women, to investigate the effects of the CDC26 gene expression on oocyte development. MAIN RESULTS AND THE ROLE OF CHANCE: Many genes were found to be differentially expressed in the oocytes of young versus old patients via RNA-seq technology. CDC26 mRNA and protein levels in aged oocytes were severely decreased, when compared with the levels observed in young oocytes. Moreover, aged oocytes lacking CDC26 were more prone to aneuploidy. These defects in aged oocytes could be partially rescued by overexpression of the CDC26 protein. LARGE SCALE DATA: N/A. LIMITATIONS, REASONS FOR CAUTION: Our study delineated key steps in

关键词： oocyte aging CDC26 meiosis APC/C infertility

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Immobilizing Graphitic Carbon Nitride on Porous Silica Via Hydrogen Bonds for Photocatalytic Flow Synthesis of Azoxybenzene

SSRN

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SSRN 2023年

作者： Liu, wuwen Zhang, Dongsheng Yue, Huiyu Li, Yajiao Rosei, Federico Liu, Dong Su, Ren Soochow University Suzhou215006 China School of Chemistry and Materials Science University of Science and Technology of China Anhui Hefei230026 China Suzhou Institute for Advanced Research University of Science and Technology of China Jiangsu Suzhou215123 China Department of Chemical and Pharmaceutical Sciences University of Trieste Via Giorgeri 1 Trieste34127 Italy

Heterogeneous photocatalysis is an ideal method for eco-friendly synthesis of value-added chemicals. However, due to the limited penetration depth of photons and inefficient utilization of the catalytic active sites, the scalability of heterogeneous photocatalysis is restrained. Combining heterogeneous photocatalysis and flow system is a promising solution to optimize the light absorption and mass transfer;however, an affordable, practical design of the flow system and the immobilization method of the photocatalyst is still missing. Here, we report a flow system for heterogeneous photocatalytic synthesis of azoxybenzene and its derivatives from reductive coupling of nitrobenzene at a gram-scale using anchored graphitic carbon nitride (gCN) on porous silica (gCN-SiO2). The hydrogen bonds formed between the gCN and the tetramethoxysilane (TMOS) precursor during the dehydrative condensation process prevent unwanted leaching of catalyst, enabling photocatalytic flow synthesis of azoxybenzene with high selectivity, efficiency, and durability. Our work provides an alternative path towards large scale heterogeneous photocatalysis. © 2023, The Authors. All rights reserved.

关键词： Hydrogen bonds

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