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Detection of changes in biological characteristics of Aeromonas veronii TH0426 after deletion of lsrB gene by homologous recombination

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MICROBIAL PATHOGENESIS 2023年 174卷 105938页

作者： Gao, Hongmiao Wang, Hong Zhang, Meng Shan, Xiaofeng Qian, Aidong Zhang, Lei Zhang, Dongxing Sun, wuwen Jilin Agr Univ Coll Vet Med Coll Anim Sci & Technol Jilin Prov Engn Res Ctr Anim ProbiotKey Lab Anim Changchun 130118 Jilin Peoples R China

Aeromonas veronii is a widespread pathogenic microorganism that can infect humans, animals, and a variety of aquatilia, at the same time, can cause diseases, mainly sepsis and ulcer syndrome. In this research, we first deleted the gene of lsrB's nucleotide sequences by homologous recombination. The results showed that the median lethal dose (LD50) of the mutant strain (Delta lsrB) for zebrafish was 1.28-times higher than that of the TH0426 strain. The toxicity of TH0426 to epithelioma papulosum cyprini (EPC) cells was 1.15-times and 1.64-times higher than that of Delta lsrB, 1 and 2 h after infection. The production ability of the biofilm of Delta lsrB decreased by 1.38-times, and the adhesion ability of Delta lsrB to EPC cells greatly decreased by 1.96-times than the TH0426. The result of motility detection pointed out that the swimming ability of Delta lsrB was down by 1.67-times. The results indicated that almost all of them lost their flagella after deleting the lsrB gene. In general, the virulence of TH0426 was reduced after deleting the lsrB gene. The final results point out that the lsrB gene of TH0426 is related to motility, biofilm formation, adhesion, and virulence.

关键词： Aeromonas veronii TH0426 strain lsrB gene Motility Virulence

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Correction of the pathogenic mutation in TGM1 gene by adenine base editing in mutant embryos

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MOLECULAR THERAPY 2022年第1期30卷 175-183页

作者： Dang, Lu Zhou, Xueliang Zhong, Xiufang Yu, Wenxia Huang, Shisheng Liu, Hanyan Chen, Yuanyuan Zhang, wuwen Yuan, Lihua Li, Lei Huang, Xingxu Li, Guanglei Liu, Jianqiao Tong, Guoqing Guangzhou Med Univ Affiliated Hosp 3 Key Lab Major Obstetr Dis Guangdong Prov Dept Obstet & GynecolCtr Reprod Med Guangzhou Peoples R China Guangzhou Med Univ Affiliated Hosp 3 Key Lab Reprod Med Guangdong Prov Guangzhou Peoples R China Shanghai Univ Tradit Chinese Med Shuguang Hosp Dept Reprod Ctr Shanghai 201203 Peoples R China ShanghaiTech Univ Gene Editing Ctr Sch Life Sci & Technol Shanghai 201210 Peoples R China

A couple diagnosed as carriers for lamellar ichthyosis, an autosomal recessive rare disease, encountered two pregnancy losses. Their blood samples showed the same heterozygous c.607C>T mutation in the TGM1 gene. However, we found that about 98.4% of the sperm had mutations, suggesting possible de novo germline mutation. To explore the probability of correcting this mutation, we used two different adenine base editors (ABEs) combined with related truncated single guide RNA (sgRNA) to repair the pathogenic mutation in mutant zygotes. Our results showed that the editing efficiency was 73.8% for ABEmax-NG combined with 20-bp-length sgRNA and 78.7% for Sc-ABEmax combined with 19-bp-length sgRNA. The whole-genome sequencing (WGS) and deep sequencing analysis demonstrated precise DNA editing. This study reveals the possibility of correcting the genetic mutation in embryos with the ABE system.

关键词： correction pathogenic mutation TGM1 gene adenine base editing mutant embryos

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Interactions between gut microbiota and polyphenols: A mechanistic and metabolomic review

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PHYTOMEDICINE 2023年 119卷 154979页

作者： Cheng, Hao Zhang, Dandan Wu, Jing Liu, Juan Zhou, Yaochuan Tan, Yuzhu Feng, wuwen Peng, Cheng Chengdu Univ Tradit Chinese Med Sch Pharm State Key Lab Southwestern Chinese Med Resources Chengdu Peoples R China Chengdu Univ Tradit Chinese Med Sch Pharm Minist Educ Key Lab Standardizat Chinese Herbal Med Chengdu Peoples R China Hosp Chengdu Univ Tradit Chinese Med Chengdu 610072 Peoples R China Chengdu Univ Tradit Chinese Med Sch Basic Med Sci Chengdu 611137 Peoples R China Chengdu Univ Tradit Chinese Med Sch Pharm State Key Lab Characterist Chinese Med Resources S Chengdu Peoples R China

Background: Polyphenols are a class of naturally sourced compounds with widespread distribution and an extensive array of bioactivities. However, due to their complex constituents and weak absorption, a convincing explanation for their remarkable bioactivity remains elusive for a long time. In recent years, interaction with gut microbiota is hypothesized to be a reasonable explanation of the potential mechanisms for natural compounds especially polyphenols. Objectives: This review aims to present a persuasive explanation for the contradiction between the limited bioavailability and the remarkable bioactivities of polyphenols by examining their interactions with gut microbiota. Methods: We assessed literatures published before April 10, 2023, from several databases, including Scopus, PubMed, Google Scholar, and Web of Science. The keywords used include "polyphenols", "gut microbiota", "short-chain fatty acids", "bile acids", "trimethylamine N-oxide", "lipopolysaccharides" "tryptophan", "dopamine", "intestinal barrier", "central nervous system", "lung", "anthocyanin", "proanthocyanidin", "baicalein", "caffeic acid", "curcumin", "epigallocatechin-3-gallate", "ferulic acid", "genistein", "kaempferol", "luteolin", "myricetin", "naringenin", "procyanidins", "protocatechuic acid", "pterostilbene", "quercetin", "resveratrol", etc. Results: The review first demonstrates that polyphenols significantly alter gut microbiota diversity (alpha- and beta-diversity) and the abundance of specific microorganisms. Polyphenols either promote or inhibit microorganisms, with various factors influencing their effects, such as dosage, treatment duration, and chemical structure of polyphenols. Furthermore, the review reveals that polyphenols regulate several gut microbiota metabolites, including short-chain fatty acids, dopamine, trimethylamine N-oxide, bile acids, and lipopolysaccharides. Polyphenols affect these metabolites by altering gut microbiota composition, modifying microbial enzyme

关键词： Polyphenols Gut microbiota Short-chain fatty acids Bile acids Tryptophan Intestinal barrier

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电网变电运维风险管控及技术检修措施研究

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电工技术 2024年第S01期 360-362,365页

作者：杜伍文臧伶俐国网新疆电力有限公司阿克苏供电公司新疆阿克苏843000

在电网变电运维领域,风险管控和技术检修是维持系统稳定和安全运行的关键。随着技术的发展,特别是智能化和自动化技术的应用,电网变电运维面临着新的挑战和机遇。首先阐述了电网变电运维风险管控及技术检修的重要性,然后深入分析当前电... 详细信息

在电网变电运维领域,风险管控和技术检修是维持系统稳定和安全运行的关键。随着技术的发展,特别是智能化和自动化技术的应用,电网变电运维面临着新的挑战和机遇。首先阐述了电网变电运维风险管控及技术检修的重要性,然后深入分析当前电网变电运维中的主要风险隐患问题,包括自然环境等外部风险、工作人员操作不规范、电网变电运维设备缺陷设备老化等因素。然后,提出电网变电运维风险管控及技术检修措施,包括建立健全变电运维管理体系、提升工作人员的风险防控意识、科学运用检测技术和方法、加强运维全过程精细化管控等,旨在为电网变电运维的风险管控及技术检修提供参考,以适应智能化时代的要求,确保电网系统的高效和安全运行。

关键词：电网变电运维风险隐患问题技术检修措施

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Interactions between polysaccharides and gut microbiota: A metabolomic and microbial review

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FOOD RESEARCH INTERNATIONAL 2022年第0期160卷 111653页

作者： Zhang, Dandan Liu, Juan Cheng, Hao Wang, Hongliang Tan, Yuzhu Feng, wuwen Peng, Cheng Chengdu Univ Tradit Chinese Med Sch Pharm State Key Lab SouthWestern Chinese Med Resources Chengdu 611137 Peoples R China Chengdu Univ Tradit Chinese Med Key Lab Minist Educ Standardizat Chinese Med Chengdu 611137 Peoples R China Chengdu Univ Tradit Chinese Med Sch Basic Med Sci Chengdu 611137 Peoples R China

In recent years, gut microbiota has become a hot topic because of its important role in health and diseases. Gut microbiota can produce a series of metabolites such as short chain fatty acids (SCFAs), secondary bile acids, tryptophan and indole derivatives. Over the past decade, a large number of studies have shown that oral polysaccharides can be transported to the colon and interact with gut microbiota. However, comprehensive summarization of the interactions between polysaccharides and gut microbiota is still lacking, especially from the perspective of other gut microbiota metabolites such as trimethylamine and tryptophan. In this review, we first summarized the comprehensive interactions between polysaccharides and gut microbiota, including (1) modulation of gut microbiota composition by polysaccharides, (2) gut microbiota metabolizes polysaccharides to SCFAs, and (3) polysaccharides modulate the production of gut microbiota metabolites including trimethylamine, tryptophan, lipopolysaccharides, etc. In addition, we also discussed the indirect effects of polysaccharides on intestinal barriers and gave our perspectives on future research on polysaccharides. This review not only helps explain the underlying mechanism of polysaccharides, but also provides a reasonable reference for the better utilization of polysaccharides.

关键词： Polysaccharides Gut microbiota Short chain fatty acids Trimethylamine-N-oxide Intestinal barriers

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Fish-derived lactic acid bacteria supplementation enhanced the immunity and resistance in Crucian carp (Carassius auratus)

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AQUACULTURE REPORTS 2024年 36卷

作者： Li, Ruoming Zhang, Meng Zhou, Yuhui Wei, Daimin Yang, Yinghui Gao, Dongpeng Shan, Xiaofeng Sun, wuwen Dong, Hao Wang, Guiqin Jilin Agr Univ Coll Anim Sci & Technol Changchun 130118 Jilin Peoples R China Jilin Agr Univ Coll Life Sci Changchun 130118 Jilin Peoples R China

This study attempted to evaluate the potential for biofilm formation and the probiotic properties of lactic acid bacteria (LAB) isolated from the Carassius auratus intestine and to determine their antibacterial activity against crucial injurious bacteria of fish. The biological features test was carried out to obtain LAB with well -resistant pH, trypsin, bile and antibiotic challenge, and biofilm formation capacity. The most promising LAB isolates, y11 and y78 were identified as Lactobacillus johnsonii and Weissella confusa using 16S ribosomal RNA gene sequencing. We discovered that oral LAB additives improved immunity in the C. auratus by increasing immune -related expression of immunoglobulin M (IgM), superoxide dismutase (SOD), alkaline phosphatase (AKP), and lysozyme (LYS) in serum and interleukins (IL -10 and IL -1(i), interferon-gamma (IFN-gamma), and tumor necrosis factor-alpha (TNF-alpha) in organs. After the introduction of Aeromonas veronii TH0426 for 15 days, the survival rate of C. auratus was determined as follows: the protection rate of group y11 was 45 %, and group y78 was 65 % when challenged with 10 9 CFU/mL density. This experiment successfully selected two LAB strains as the bioprotective agents and provided theoretical support for further LAB application in aquaculture.

关键词： Carassius auratus Lactic acid bacteria Biofilm Immunity Bacteriostatic ability

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基于有限元形函数的钻孔水头插值方法

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水资源与水工程学报 2021年第2期32卷 202-208页

作者：钱武文肖芳南昌工程学院水利与生态工程学院江西南昌330029

水头观测数据对于水工渗流参数反演至关重要。针对观测点位置不在有限元网格节点上的水头插值问题,为了避免重新剖分有限元网格,提出了一套使用等参单元形函数作为插值函数的有限元插值函数法,用于插值计算插值点的水头值。为了确定插... 详细信息

水头观测数据对于水工渗流参数反演至关重要。针对观测点位置不在有限元网格节点上的水头插值问题,为了避免重新剖分有限元网格,提出了一套使用等参单元形函数作为插值函数的有限元插值函数法,用于插值计算插值点的水头值。为了确定插值点的归属单元,给出了确定点与平面单元和空间单元的拓扑关系判别法。采用改进的差分进化算法反求插值点对应的局部坐标,从而能利用单元形函数直接插值观测点的水头值。使用二维和三维算例测试了有限元插值函数法的插值性能。结果表明:计算的结果可靠,计算时长为毫秒级。

关键词：水工渗流有限元形函数拓扑关系水头插值逆变换

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Dual-channel biosensor for simultaneous detection of S. typhimurium and L. monocytogenes using nanotags of gold nanoparticles loaded metal-organic frameworks

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ANALYTICA CHIMICA ACTA 2023年 1279卷 341816页

作者： Ye, Yongkang Yan, wuwen Wang, Tingting Zhang, Chenlu Wang, Kaicheng Lu, Yuexi Zheng, Haisong Tao, Yunlai Cao, Xiaodong Li, Yunfei Hefei Univ Technol Sch Food & Biol Engn Hefei 230009 Peoples R China Technol Ctr Hefei Customs Dist Hefei 230022 Peoples R China Anhui Inst Food & Drug Inspect Hefei 230051 Peoples R China North Minzu Univ Sch Biol Sci & Engn Yinchuan 750021 Peoples R China

Simultaneous detection of multiple foodborne pathogens is of great importance for ensuring food safety. Herein, we present a sensitive dual-channel electrochemical biosensor based on copper metal organic frameworks (CuMOF) and lead metal organic framework (PbMOF) for simultaneous detection of Salmonella typhimurium (S. typhimurium) and Listeria monocytogenes (L. monocytogenes). The MOF-based nanotags were prepared by functionalizing gold nanoparticles loaded CuMOF (Au@CuMOF) and PbMOF (Au@PbMOF) with signal DNA sequences 1 (sDNA1) and sDNA2, respectively. By selecting invA of S. typhimurium and inlA gene of L. monocytogenes as targe sequences, a sandwich-typed dual-channel biosensor was developed on glassy carbon electrodes (GCE) through hybridization reactions. The sensitive detection of S. typhimurium and L. monocytogenes was achieved by the direct differential pulse voltametric (DPV) signals of Cu2+ and Pb2+. Under optimal conditions, channel 1 of the biosensor showed linear range for invA gene of S. typhimurium in 1 x 10(-14)-1 x 10(-8) M with low detection limit (LOD) of 3.42 x 10(-16) M (S/N = 3), and channel 2 of the biosensor showed linear range for inlA gene of L. monocytogenes in 1 x 10(-13)-1 x 10(-8) M with LOD of 6.11 x 10(-15) M (S/N = 3). The dual channel biosensor showed good selectivity which were used to detect S. typhimurium with linear range of 5-1.0 x 10(4) CFU mL(-1) (LOD of 2.33 CFU mL(-1)), and L. monocytogenes with linear range of 10 1.0 x 10(4) CFU mL(-1) (LOD of 6.61 CFU mL(-1)).

关键词： Dual-channel biosensor Organometallic frame Foodborne pathogenic bacteria Direct electrochemical signal Simultaneous detection

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Atomic scale analysis of sub-10 nm implantation of size-selected gold clusters into multilayer graphene

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APPLIED PHYSICS LETTERS 2024年第14期125卷

作者： Tang, Sichen Xiang, Jingye Hu, Shengyong Zuo, Zewen Lu, Siqi Zhao, Zixiang Zhang, Yongxin Zhu, wuwen Zheng, Dong Hu, Kuo-juei Zhang, Minhao Song, Fengqi Nanjing Univ Collaborat Innovat Ctr Adv Microstruct Natl Lab Solid State Microstruct Nanjing 210093 Peoples R China Nanjing Univ Sch Phys Nanjing 210093 Peoples R China Atom Mfg Inst AMI Nanjing 211805 Peoples R China

Here, we report the sub-10 nm implantation of size-selected AuN nanoclusters (N = 20-147) into multilayer graphene with different deposition energy (50-15 000 eV) using a magnetron sputtering cluster beam source. Combined with aberration-corrected scanning transmission electron microscopy, we directly characterize the implantation depth of gold clusters and demonstrate the scaling relations between size, deposition energy, and implantation depth of gold clusters. The time-of-flight secondary ion mass spectrometry analysis can also confirm the possibility of ultra-shallow implantation. The results are further corroborated by molecular dynamics simulations of Au-55, which show that at a deposition energy of 15 keV, Au-55 penetrates about 4.45 nm in multilayer graphene, consistent with our experimental result of 4.38 nm, i.e., about 13 atomic layers. Our results reveal the possibility of cluster implantation to atomic precision, thus guiding the fabrication of sub-10 nm electronic devices.

关键词： Secondary ion mass spectrometry

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Effects of body mass index on IVF outcomes in different age groups

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BMC WOMENS HEALTH 2023年第1期23卷 416-416页

作者： Liu, Dan Li, Li Sun, Ningyu Zhang, Xiaole Yin, Ping Zhang, wuwen Hu, Panwei Yan, Hua Zhang, Qinhua Affiliated Shanghai Univ Tradit Chinese Med Shuguang Hosp Dept Reprod Med Shanghai Peoples R China Affiliated Shanghai Univ Tradit Chinese Med Shuguang Hosp Dept Gynecol Shanghai Peoples R China

BackgroundHerein, we aimed to analyse the effects of body mass index (BMI) on the treatment outcomes of in vitro fertilisation (IVF) in a cohort of women undergoing their first IVF *** total of 2311 cycles from 986 women undergoing their first IVF/intracytoplasmic sperm injection cycle with fresh/frozen embryo transfer between January 2018 and December 2021 at the Center of Reproductive Medicine, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, were considered in this retrospective cohort study. First, the included patients were classified into four groups based on their BMI: underweight (BMI < 18.5 kg/m(2), 78 patients), normal weight (18.5 & LE;BMI < 24 kg/m(2), 721patients), overweight (24 & LE;BMI < 28 kg/m(2), 147 patients), and obese (BMI & GE;28 kg/m(2), 40 patients). The IVF outcomes included the Gn medication days;Gn dosage;number of retrieved oocytes, mature oocytes, fertilized oocytes, cleavages, and available embryos and high-quality embryos;implantation rate;clinical pregnancy rate and live birth rate. Next, all the obtained data were segregated into three different subgroups according to the patient age: < 30 years, 30-38 years and > 38 years;the IVF pregnancy outcomes were compared among the *** with the other three groups, the underweight group had a higher number of fertilized oocytes, cleavage and available embryos and a smaller Gn medication days and required a lower Gn dosage. There was no difference in the number of retrieved oocytes and mature oocytes among the groups. Moreover, compared with the women aged 30-38 years in the overweight group, those in the normal weight group had a significantly higher implantation rate, clinical pregnancy rate and live birth rate (p = 0.013 OR 1.75, p = 0.033 OR 1.735, p = 0.020 OR 1.252 respectively). The clinical pregnancy rate was also significantly higher in those aged 30-38 years in the normal weight group than in the obese group (p = 0.036

关键词： BMI Age IVF Pregnancy outcomes

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