Wound healing is a complex and dynamic process involving a series of cellular and molecular events. Revascularization, the restoration of blood flow to ischemic or damaged tissue, is a key step in wound healing. Adequ...
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Wound healing is a complex and dynamic process involving a series of cellular and molecular events. Revascularization, the restoration of blood flow to ischemic or damaged tissue, is a key step in wound healing. Adequate vascularization has been recognized as a necessary factor for successful tissue regeneration. In the later stage of revascularization and tissue remodeling in wound healing, stem cells regulate other repair cells and matrix formation by influencing the maturation of blood vessels. The reductive oxidation (REDOX) state may be a key mechanism through stem/progenitor cells to influence endothelial cells to mature blood vessels and improve the quality of healing. Mitochondria may play an important role in this process.
Loss of sweat glands(SwGs)commonly associated with extensive skin defects is a leading cause of hyperthermia and heat *** vivo tissue engineering possesses the potential to take use of the body natural ability to rege...
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Loss of sweat glands(SwGs)commonly associated with extensive skin defects is a leading cause of hyperthermia and heat *** vivo tissue engineering possesses the potential to take use of the body natural ability to regenerate SwGs,making it more conducive to clinical *** recent advances in regenerative medicine,reconstructing SwG tissue with the same structure and function as native tissue remains *** the SwG generation mechanism and developing biomaterials for in vivo tissue engineering is essential for understanding and developing in vivo SwG regenerative ***,we outline the cell biology associated with functional wound healing and the characteristics of bioactive *** critically summarize the recent progress in bioactive material-based cell modulation approaches for in vivo SwG regeneration,including the recruitment of endogenous cells to the skin lesion for SwG regeneration and in vivo cellular reprogramming for SwG *** discussed the re-establishment of microenvironment via bioactive material-mediated ***,we offer promising perspectives for directing in situ SwG regeneration via bioactive material-based cell-free strategy,which is a simple and effective approach to regenerate SwG tissue with both fidelity of structure and ***,we discuss the opportunities and challenges of in vivo SwG regeneration in *** molecular mechanisms and cell fate modulation of in vivo SwG regeneration will provide further insights into the regeneration of patient-specific SwGs and the development of potential intervention strategies for gland-derived diseases.
Endogenous regeneration is becoming an increasingly important strategy for wound healing as it facilitates skin’s own regenerative potential for self-healing,thereby avoiding the risks of immune rejection and exogeno...
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Endogenous regeneration is becoming an increasingly important strategy for wound healing as it facilitates skin’s own regenerative potential for self-healing,thereby avoiding the risks of immune rejection and exogenous ***,currently applied biomaterials for inducing endogenous skin regeneration are simplistic in their structure and function,lacking the ability to accurately mimic the intricate tissue structure and regulate the disordered *** biomimetic biomaterials with precise structure,chemical composition,and biophysical properties offer a promising avenue for achieving perfect endogenous skin ***,we outline the recent advances in biomimetic materials induced endogenous skin regeneration from the aspects of structural and functional mimicry,physiological process regulation,and biophysical property ***,novel techniques including in situ reprograming,flexible electronic skin,artificial intelligence,single-cell sequencing,and spatial transcriptomics,which have potential to contribute to the development of biomimetic biomaterials are ***,the prospects and challenges of further research and application of biomimetic biomaterials are *** review provides reference to address the clinical problems of rapid and high-quality skin regeneration.
Even with many advances in design strategies over the past three decades,an enormous gap remains between existing tissue engineering skin and natural *** available in vitro skin models still cannot replicate the three...
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Even with many advances in design strategies over the past three decades,an enormous gap remains between existing tissue engineering skin and natural *** available in vitro skin models still cannot replicate the three-dimensionality and heterogeneity of the dermal microenvironment sufficiently to recapitulate many of the known characteristics of skin disorder or disease in ***-dimensional(3D)bioprinting enables precise control over multiple compositions,spatial distributions and architectural complexity,therefore offering hope for filling the gap of structure and function between natural and artificial *** understanding of wound healing process and skin disease would thus be boosted by the development of in vitro models that could more completely capture the heterogeneous features of skin ***,we provide an overview of recent advances in 3D skin bioprinting,as well as design concepts of cells and bioinks suitable for the bioprinting *** focus on the applications of this technology for engineering physiological or pathological skin model,focusing more specifically on the function of skin appendages and *** conclude with current challenges and the technical perspective for further development of 3D skin bioprinting.
Chitosan is an excellent carrier material for bioactive substances, and its binding ability is affected by the pH value of surrounding environments. Healthy skin is maintained in a slightly acidic environment, whereas...
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Chitosan is an excellent carrier material for bioactive substances, and its binding ability is affected by the pH value of surrounding environments. Healthy skin is maintained in a slightly acidic environment, whereas the wound healing environment is normally neutral or slightly alkaline. In the present study, the authors proposed developing a thermally stable bioactive chitosan scaffold (T-CS) with pH-responsive exosome adsorption and release functions to promote wound healing. Our results revealed that T-CS could automatically capture exosomes from human umbilical cord mesenchymal stem cells in an acidic environment and release them in alkaline or neutral environments. The exosomes separated by T-CS and the traditional ultracentrifugation (UC) method exhibited similar size and protein markers. furthermore, the exosomal biological activities of the T-CS (T-CS-E) and UC groups exhibited similar anti-inflammatory, proproliferation, promigration, and proendothelial cell-tube formation effects on human umbilical vein endothelial cells. Similar results were achieved in a mouse model by sustainably releasing exosomes. T-CS-E could facilitate wound healing by enhancing cell proliferation, inhibiting wound inflammation, and promoting vascularization. Therefore, this study developed a T-CS scaffold that integrates exosome isolation and application for wound healing, laying the foundation for future clinical use.
Titanium mesh exposure after cranioplasty is the most serious complication of this *** some clinical experience has been gradually accumulated over the years in the diagnosis and treatment of titanium mesh exposure,th...
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Titanium mesh exposure after cranioplasty is the most serious complication of this *** some clinical experience has been gradually accumulated over the years in the diagnosis and treatment of titanium mesh exposure,the treatment is often not standardized and it is difficult to achieve satisfactory repair results due to insufficient understanding of its pathogenesis and concurrent *** normalize the diagnosis and treatment of titanium mesh exposed wounds after cranioplasty and improve the therapeutic effect and the quality of life of patients,the Wound Repair Professional Committee of Chinese Medical Doctor Association organized an expert discussion based on the literature and current diagnosis and treatment status of titanium mesh exposed wounds after cranioplasty at home and abroad,and reached a consensus on the pathogenesis,preventive measures,and diagnosis and treatment strategies of titanium mesh exposed wounds after cranioplasty to provide reference for relevant clinicians.
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