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A Low-cost and Rapid Method for Determination of Five Flavonoids in Herbal Medicines with Rutin by HPLC-UV at Equal Absorption Wavelength

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CURRENT ANALYTICAL CHEMISTRY 2025年第2期21卷 124-133页

作者： Qian, Zhengming He, Zhuobin Lian, Dongyin Huang, Qi Tan, Guoying xie, juying Xiangnan Univ Coll Med Imaging Lab & Rehabil Chenzhou Peoples R China Dongguan HEC Cordyceps R&D Co Ltd Key Lab State Adm Tradit Chinese Med Dongguan Peoples R China

Background Flavonoid is a type of active constituent in herbs and always used as the quality control markers of herbal medicines. Owing to the extensive diversity of flavonoids, numerous reference compounds are necessitated for the analysis of flavonoids, and some are usually very expensive, which engenders challenges in the analysis of flavonoids in herbal medicines. Consequently, the development of a simple, rapid, and reference compounds saving method is important for the determination of flavonoids in herbal *** In order to develop a high-performance liquid chromatography (HPLC) method for the determination of 5 flavonoids (mangiferin, hesperidin, baicalin, buddleoside, and rutin) in five herbal medicines (Anemarrhenae rhizome, Sophorae flos, Citri reticulatae pericarpium, Scutellariae radix, and Chrysanthemi indici flos) with *** Five herbal medicine samples were prepared according to the Chinese Pharmacopoeia which includes ultrasound and reflux methods. The separation of the sample was performed on a PoroShell 120 EC-C18 (4.6 mmx100 mm, 2.7 mu m) by gradient elution with 0.1% formic acid and acetonitrile at a flow rate of 1.0 mL/min. The wavelengths were set as follows: Anemarrhenae rhizome (363 nm), Sophorae flos (256 nm), Citri reticulatae pericarpium (236 nm), Scutellariae radix (263 nm), Chrysanthemi indici flos (354 nm).Results The method validation showed that the established HPLC method was accurate and stable for quantitative analysis of flavonoids in five herbal medicines. The comparative analysis revealed that the determination results of the current HPLC method and Chinese Pharmacopoeia method are consistent, exhibiting less than 1% relative error. Remarkably, the developed HPLC method needs one cheapest reference compound (rutin) and costs 8 min for sample HPLC *** The developed HPLC method for quantitative analysis of five flavonoids in five herbal medicines is simple, rapid, and reference compound saving

关键词： Flavonoids rutin herbal medicine HPLC equal absorption wavelength quantitative analysis quality control

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Elucidating the role of KCTD10 in coronary atherosclerosis: Harnessing bioinformatics and machine learning to advance understanding

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SCIENTIFIC REPORTS 2025年第1期15卷 1-18页

作者： Hu, Xiaomei Liang, Fanqi Zheng, Man xie, juying Wang, Shanxi Xiangnan Univ Coll Med Imaging Lab & Rehabil Chenzhou 423000 Hunan Peoples R China Xiangnan Univ Rehabil Dept Affiliated Hosp 25 Peoples West Rd Chenzhou 423000 Hunan Peoples R China Hunan Univ Tradit Chinese Med Affiliated Hosp 1 Changsha 410007 Hunan Peoples R China Dongying Peoples Hosp Dongying Hosp Shandong Prov Hosp Grp Dongying 257091 Shandong Peoples R China

Atherosclerosis (AS) is increasingly recognized as a chronic inflammatory disease that significantly compromises vascular health and serves as a major contributor to cardiovascular diseases. KCTD10, a protein implicated in a variety of biological processes, has garnered significant attention for its role in cardiovascular diseases and metabolic regulation. As a member of the KCTD protein family, KCTD10 is characterized by the presence of a T1 domain that interacts with voltage-gated potassium channels, a critical interaction for modulating channel activity and intracellular signal transduction. In our study, KCTD10 was identified as a focal point through an integrative analysis of differentially expressed genes (DEGs) across multiple datasets (GSE43292 and GSE9820) from the GEO database, aligned with immune-related gene sets from the ImmPort database. Advanced analytical tools, including Lasso regression and Support Vector Machine-Recursive Feature Elimination (SVM-RFE), were employed to refine our gene selection. We further applied Gene Set Enrichment Analysis (GSEA) and Gene Set Variation Analysis (GSVA) to these gene sets, revealing significant enrichment in immune-related pathways. The relationship between KCTD10 expression and immune processes was examined using CIBERSORT and ESTIMATE algorithms to assess tumor microenvironment characteristics, suggesting increased immune cell infiltration associated with higher KCTD10 expression. Validation of these findings was conducted using data from the GSE9820 dataset. Among 10 DEGs linked with KCTD10, 13 were identified as hub genes through LASSO and SVM-RFE analyses. Functional assays highlighted KCTD10's role in enhancing viral defense mechanisms, cytokine production, and immune cascades. Notably, KCTD10 expression correlated positively with several immune cells, including naive CD4 + T cells, eosinophils, resting NK cells, neutrophils, M0 macrophages, and particularly M1 macrophages, indicating a significant associat

关键词： Coronary Atherosclerosis (AS) KCTD10 Lasso regression SVM-RFE Autoimmune inflammatory disorder

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A new longwave radiation model for application to atmospheric problems

A new longwave radiation model for application to atmospheri...

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作者： Warner, juying xie University of Maryland College Park

学位级别：Ph.D.

The accuracy of radiation models has become a critical issue in climate studies. In this dissertation, eight existing research and operation radiation models are compared against the accurate surface observations with high spectral resolution to determine the absolute accuracy of the current models. Moreover, a new accurate narrow band longwave radiative transfer model for clear-sky conditions is developed. The model is used to calculate the case study sensitivities previously studied by the international InterComparison of Radiation Codes used in Climate Models (ICRCCM), particularly the sensitivities of the important greenhouse gases water vapor and carbon dioxide. In the model development we first show that traditional techniques for estimating Malkmus statistical model parameters from the line compilation and line-by-line models can not be trusted to give accurate transmittance function. We then describe a new technique that calculates water vapor line transmittances with good agreement with line-by-line (LBLRTM) calculations (i.e., with rms errors less than 0.01 for more than 97% of the intervals). The water vapor continuum is included in a manner consistent with the water vapor line absorption. The modeling of the gases CO$\sb2$, O$\sb3$, N$\sb2$O and CH$\sb4$ adopts the Malkmus formula with the parameters fit to LBLRTM transmittances. Fluxes calculated with the model agree with LBLRTM to about 1 W/m$\sp2$ for the entire vertical range of the atmosphere for several test cases. The heating rate errors are reduced by as much as 0.25$\sp\circ$C/day below the tropopause for the test cases compared with the original narrow band model. The model is validated with surface observations, and is compared with the other models. Both LBLRTM and the new model are documented for ICRCCM test cases, and the performances of the 'average' ICRCCM climate models are evaluated with respect to the LBLRTM calculations.

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Analysis of ginseng root and leaf by multiple columns and detections liquid chromatography

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JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 2020年第13-14期43卷 464-473页

作者： Qian, Zhengming Huang, Qi Li, Chunhong Chen, Jing Li, Gang Ma, Hongyan xie, juying Xiangnan Univ Sch Rehabil Chenzhou 423000 Peoples R China Sunshine Lake Pharma Co LTD Key Lab State Adm Tradit Chinese Med Dongguan Peoples R China Jilin Acad Agr Sci Agr Environm & Resources Res Ctr Changchun Peoples R China Guangdong Pharmaceut Univ Sch Tradit Chinese Med Guangzhou 510006 Peoples R China

A multiple columns and detections liquid chromatography system, including size exclusion chromatography (SEC) and reversed phase liquid chromatography (RPLC), for the analysis of macromolecules and micromolecules in ginseng root and leaf was developed. The columns were connected by two switching valves. Macromolecules were separated on a SEC column (TSK gel SuperMultipore PW-H column, 6 mmx 150 mm, 8 mu m) by isocratic elution of 50 mM ammonium acetate aqueous solution, 0.3 mL/min of flow rate and detected by evaporative light scattering detection (ELSD). Micromolecules were analyzed on a Poroshell RP column (Agilent Poroshell 120 SB-Aq column, 4.6 mm x 50 mm, 2.7 mu m) with gradient elution of water and acetonitrile, 0.6 mL/min of flow rate and detected by ultraviolet detection (UV). As a result, in the macromolecules chromatogram of ginseng root sample showed two main peaks while only one major peak for ginseng leaf. For micromolecules analysis, 17 compounds (3 nucleosides + 14 saponins) and 17 compounds (3 nucleosides + 1 flavonoid + 13 saponins) were found in ginseng root and leaf, respectively. The developed method is helpful for the quality evaluation of ginseng root and leaf.

关键词： Ginseng leaf ginseng root HPLC multiple columns multiple detections

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The Effect of Kinesitherapy on Bone Mineral Density in Primary Osteoporosis: A Systematic Review and Meta-Analysis of Randomized Controlled Trial

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EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2020年第1期2020卷 5074824页

作者： Wang, Shanxi Li, Shuzhen xie, Xing xie, juying Xiangnan Univ Coll Rehabil Chenzhou 423000 Peoples R China Xiangnan Univ Affiliated Hosp Chenzhou 423000 Peoples R China

Objective. Osteoporosis (OP) is a well-established age-related disease, pathologically characterized by bone microarchitectural deterioration, increased fragility, and low BMD. Primary osteoporosis (POP) is the most common type of ***. Publications pertaining to the effectiveness of kinesitherapy on BMD in POP from PubMed, SCI, Cochrane Library, Embase, VIP, CNKI, and Wanfang Database were retrieved from their inception to October ***. A total of 21 studies with 1840 participants were included. The results of the meta-analysis revealed that kinesitherapy plus antiosteoporosis medications had a positive effect on lumbar spine BMD when the duration of intervention was 6 months (MD = 0.11 g/cm(2);95% CI: 0.06-0.15;P<0.0001) or >6 months (MD = 0.04 g/cm(2);95% CI: 0.02-0.06;P<0.0001) compared with antiosteoporosis medications alone. Additional kinesitherapy plus antiosteoporosis medications were associated with improved femoral neck BMD compared with antiosteoporosis medications alone (MD = 0.09 g/cm(2);95% CI: 0.03-0.16;P=0.004).Conclusions. Kinesitherapy plus antiosteoporosis medications significantly improved lumbar spine and femoral neck BMD in the current low-quality evidence. Additional high-quality evidence is required to confirm the effect of kinesitherapy on BMD in patients with POP.

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High performance multichannel scaler based on C8051F020 MCU

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He Jishu/Nuclear Techniques 2008年第5期31卷 379-383页

作者： Huang, Shun Zhao, Xiuliang Zhao, Yanhui xie, juying Yu, Hong Zhang, Meiqin School of Nuclear Science and Technology Nanhua University Hengyang 421001 China Department of Physics and Electronic Information Science Hengyang Normal University Hengyang 421008 China School of Electrical Engineering Nanhua University Hengyang 421001 China

A sixteen-channel scaler was designed with a C8051F020 microcontroller unit (MCU) and INTEL 8254-2 timer-counter chips. Software controlling techniques were used to increase the counting capacity up to 2.8 × 1014 by using software counters, to correct errors caused by frequency changes of the crystal oscillator by adjusting software timer to improve the timing accuracy, and to avoid the potential interference by designing digital filters. In addition, the maximal counting rate reached 1 × 107 s-1.

关键词： C8051F020 Counting capacity INTEL8254-2 Maximal counting rate Software filter

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Rapid determination of multiple components in herbal medicine using a single reference compound by high-performance liquid chromatography at equal absorption wavelength: Case study of Magnoliae officinalis cortex

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SEPARATION SCIENCE PLUS 2024年第1期7卷

作者： Qian, Zhengming Chen, Jing Lei, Qinggui Tan, Guoying Zou, Yuansheng Peng, Gang xie, juying Li, Wenqing Xiangnan Univ Coll Med Imaging Lab & Rehabil Chenzhou Peoples R China Dongguan HEC Cordyceps R&D Co Ltd Key Lab State Adm Tradit Chinese Med Dongguan Peoples R China Lingnan Tradit Chinese Med Tablets Co Ltd Guangzhou Peoples R China

Herbal medicine is a complex system containing numerous bioactive components. Evaluating the quality of herbs often requires analyzing multiple components using high-performance liquid chromatography (HPLC). However, existing HPLC methods are time-consuming and consume large amounts of reference compounds. This study, an ultra-rapid and green assay method for the determination of honokiol and magnolol in Magnoliae Officinalis Cortex using a single reference compound was developed by HPLC at equal absorption wavelength (EAW). The sample was prepared using ultrasonic-assisted matrix solid-phase dispersion and separated with an eco-friendly mobile phase on the Poroshell C18 column. The EAW of homokiol and magnolol was chosen as the detecting wavelength (247 nm). The contents of honokiol and magnolol in six Magnoliae Officinalis Cortex samples obtained by the developed method with a single marker and the external standard method with two markers were comparable. Additionally, the developed HPLC process only needed 4.55 mL green organic solution (ethanol) and 2.5 min, which included sample extraction and separation. The developed HPLC EAW method was ultra-fast, green, and reference compound saving, which was an improved quality control method of Magnoliae Officinalis Cortex and would serve as an example for the determination of multiple components in other herbal medicines using a single reference compound.

关键词： equal absorption wavelength green high-performance liquid chromatography matrix solid-phase dispersion rapid

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加强高校艺术设计专业学生的文化素质培养

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中国科技信息 2008年第21期 271-271页

作者：刘青谢菊英江西农业大学园林与艺术学院

针对艺术设计专业学生存在的文化素质相对较差的特点,深入分析其内在成因,并提出相应的解决方法,为艺术设计专业的深入发展铺陈道路。

关键词：文化素质教学培养艺术设计

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高速公路景观与绿化设计初探

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江西林业科技 2006年第2期34卷 56-58页

作者：刘青谢菊英江西农业大学林学院江西南昌330045

为了把高速公路建成具有个性特色和良好生态的绿色生态走廊,从当前高速公路景观设计中存在的不足出发,对泰井高速公路景观设计的原则与内容进行分析,提出高速公路景观设计要注重生态恢复和路域景观特色构建,并提出一些相应的措施和手段。

关键词：高速公路生态恢复生态走廊景观设计分

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高效液相色谱紫外等吸收波长法快速测定秦皮中秦皮甲素和秦皮乙素

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色谱 2023年第8期41卷 690-697页

作者：钱正明吴梦奇谭国英金李玲李宁谢菊英湘南学院医学影像检验与康复学院湖南郴州423000 东莞市东阳光冬虫夏草研发有限公司广东东莞523850 云南中医药大学中药学院云南昆明650500

研究建立了一种快速、环保和节约对照品的秦皮化学成分含量测定方法。秦皮样品采用25%(v/v)乙醇水溶液超声提取5 min。样品提取溶液采用Poroshell 120 EC-C18色谱柱(50 mm×4.6 mm, 2.7μm)进行分析,0.1%甲酸水溶液-乙腈(94∶6, v/v... 详细信息

研究建立了一种快速、环保和节约对照品的秦皮化学成分含量测定方法。秦皮样品采用25%(v/v)乙醇水溶液超声提取5 min。样品提取溶液采用Poroshell 120 EC-C18色谱柱(50 mm×4.6 mm, 2.7μm)进行分析,0.1%甲酸水溶液-乙腈(94∶6, v/v)等度洗脱,流速1.5 mL/min。采用紫外分光光度仪对等浓度的秦皮甲素和秦皮乙素进行紫外光谱扫描,初步筛选得到2个对照品的等吸收波长,并用高效液相色谱仪对筛选得到的等吸收波长进行确证,从而获得2个对照品的等吸收检测波长341 nm。新建立的高效液相色谱紫外等吸收波长法的方法验证结果显示,秦皮甲素和秦皮乙素在各自的浓度范围内具有良好的线性关系(r=1.000 0),检出限和定量限分别为1.5μmol/L和3.0μmol/L,平均加标回收率分别为99.0%(RSD=1.4%)和97.5%(RSD=0.9%)。通过比较高效液相色谱紫外等吸收波长法和传统高效液相色谱外标法对10批秦皮样品中秦皮甲素和秦皮乙素的含量测定结果,显示两种方法测定结果一致。此外还采用t检验对该方法与《中国药典》2020版方法的含量测定结果进行比较,对比结果表明两种测定方法无显著性差异(P>0.05)。该方法耗时10 min,有害溶剂消耗0.5 mL,只使用1个对照品,具有快速、简便、环保和对照品消耗少的特点,适用于秦皮中秦皮甲素和秦皮乙素的含量测定,为秦皮质量评价的技术提升提供了依据。

关键词：高效液相色谱等吸收波长核壳型色谱柱秦皮甲素秦皮乙素秦皮

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