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Gut microbiota analysis of juvenile genetically improved farmed tilapia (Oreochromis niloticus) by dietary supplementation of different resveratrol concentrations

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FISH & SHELLFISH IMMUNOLOGY 2018年 77卷 200-207页

作者： Zheng, Yao Wu, Wei Hu, Gengdong Qiu, Liping Meng, Shunlong Song, Chao Fan, Limin Zhao, Zhixiang Bing, xuwen chen, Jiazhang Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr 9 Shanshui East Rd Wuxi 214081 Jiangsu Peoples R China Minist Agr Fishery Ecoevironm Monitoring Ctr Lower Reaches Y Wuxi 214081 Jiangsu Peoples R China Minist Agr Lab Qual & Safety Risk Assessment Aquat Prod Envi Wuxi 214081 Jiangsu Peoples R China Nanjing Agr Univ Wuxi Fishery Coll Wuxi 214081 Jiangsu Peoples R China Minist Agr Key Lab Control Qual & Safety Aquat Prod Wuxi Jiangsu Peoples R China

The genetically improved farmed tilapia (GIFT, Oreochromis niloticus) is cultured widely for production of freshwater fish in China, while streptococcosis, likely related to pathogenic infections, occurs frequently in juvenile, mother, and operated GIFT. The gut microbiota plays an important role in nutrient digestibility in animals, and resveratrol (RES) has been used in feed for different freshwater fish species. Therefore, understanding changes in the tilapia gut microbiota across different concentrations of dietary RES supplementation is extremely important. The gut microbiota population in tilapia at 45 d after supplementation with different concentrations (0, 0.025, 0.05, 0.1 g/kg) of dietary RES was assessed by 16S rDNA gene sequencing. A total of 5445 operational taxonomic units were identified from all samples, and 14 phyla and 81 families were identified from all fecal samples. The bacteria of the phylum Firmicutes were significantly enriched in the 0.025 g/kg RES group when compared with the controls. Proteobacteria, Firmicutes and Cyanobacteria were the most dominant three phyla in all samples. With the increasing concentrations, the proportion of beneficial microbial taxa (Acetobacteraceae and Methylobacteriaceae) increased, whereas the proportion of harmful microbial taxa decreased, eg. Streptococcaceae except for 0.1 g/kg RES groups. RES did not affect the richness and diversity in tilapia gut microbiota. These findings provide information on the diversity and differences in GIFT gut microbiota database, and may contribute to developing strategies for management of diseases and long-term sustainability of O. niloticus culture.

关键词： Resveratrol GIFT Gut microbiota 16S rDNA Disease defense

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Production and Water Quality Responses in Tilapia Aquaculture Tanks Receiving Organic Fertilizers and Algae

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Agricultural Biotechnology 2019年第4期8卷 156-162页

作者： Shunlong MENG Dandan LI Liping QIU Gengdong HU Limin FAN Chao SONG Yao ZHENG Wei WU Jiazhang chen xuwen BING Freshwater Fisheries Research Center Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze RiverMinistry of Agriculture/Key Laboratory of Fishery Eco-environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze RiverChinese Academy of Fishery SciencesWuxi 214081China Wuxi Fishery College Nanjing Agricultural UniversityWuxi 214081China

A two-month trial was carried out to evaluate the possibility of using algae and animal manure in tilapia *** the effect of algae,chicken manure and algae mixture together,cattle manure and algae mixture together,chicken-cattle manure and algae mixture together on the water quality and tilapia production was *** results showed that the yield of tilapia were 3.46,4.33,3.81,2.92 and 3.76 kg in Control,Algae,Chicken-A,Cattle-A and C-C-A,respectively,following the order of Algae>Chicken-A>C-C-A>Control>Cattle-A,and tilapia yield in Algae and chicken manure treatment groups increased by 25.0%and 10.1%respectively compared with the *** and chicken manure could increase the tilapia production,but cattle manure has no the *** the effect of algae and animal manure on water quality showed that adding chicken manure into tilapia pond could make water quality decreased at the beginning 20 d,but could increase water quality after 20 d and it can stabilize the phytoplankton structure in aquaculture *** Chlorella vulgaris and Scenedesmus quadricauda into tilapia pond could make water quality in a good state during the aquaculture process and it can stabilize the phytoplankton structure in aquaculture water.

关键词： Algae Chicken manure Cattle manure Tilapia production Water quality

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运用多层螺旋CT探讨第一对角支斑块形成的危险因素

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中华介入放射学电子杂志 2021年第2期9卷 200-203页

作者：陈松谭文斌张慈慈段丽霞熊玉超刘雅慧曾旭文暨南大学附属广州红十字会医院放射科广东广州510220 仁伯爵综合医院影像科澳门999078

目的运用多层螺旋CT探讨第一对角支开口处斑块形成的危险因素。方法收集我院2017年5月至2020年5月同时行冠状动脉CT血管造影(CCTA)和冠状动脉造影的冠心病患者共94例,48例第一对角支开口处有斑块为观察组,46例第一对角支开口处无斑块为... 详细信息

目的运用多层螺旋CT探讨第一对角支开口处斑块形成的危险因素。方法收集我院2017年5月至2020年5月同时行冠状动脉CT血管造影(CCTA)和冠状动脉造影的冠心病患者共94例,48例第一对角支开口处有斑块为观察组,46例第一对角支开口处无斑块为对照组。分析两组的血压、前降支狭窄程度和血流速度、前降支与第一对角支夹角的差异,并分析上述因素对第一对角支斑块形成的影响。结果两组间的血压、前降支狭窄程度和血流速度、前降支与第一对角支夹角的差异具有统计学意义(P<0.05),前降支狭窄程度和血压是第一对角支开口处形成斑块的危险因素,其OR值分别为1.030、1.128。结论血压及前降支的狭窄程度是第一对角支斑块形成的危险因素,且随着血压的增高,前降支狭窄程度的增大,第一对角支开口处斑块的发生率增加。

关键词：多层螺旋CT 冠心病对角支斑块

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An Decomposition Algorithm for Distribution Network Reconfiguration Schedule Considering Demand Response 10

An Decomposition Algorithm for Distribution Network Reconfig...

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IEEE PES Asia-Pacific Power and Energy Engineering Conference (APPEEC)

作者： Li, Zihao Wu, Wenchuan Zhang, Boming Tai, Xue Yang, Jinxi Han, xuwen Zhang, chen Qian, Yuhao Wang, Bo Tsinghua Univ Dept Elect Engineer Beijing 100084 Peoples R China Elect Power Co Ltd State Grid Jiangsu Yangzhou Yangzhou 225000 Jiangsu Peoples R China

ISBN: (纸本)9781538656860

Network reconfiguration and demand response can both reduce the loss and improve the security of the distribution networks (DNs). In this paper, we describe a day-ahead DN reconfiguration schedule model considering demand response, in which network reconfiguration is conducted by distribution system operator (DSO) while the demand response strategies are realized by customer aggregators. To preserve information privacy and reduce computational burden, a two-level decomposition & coordination algorithm is proposed to solve this model, which is based on a log-barrier cost function. In the upper level, the DSO optimizes the network reconfiguration schedule, calculates the barrier cost of every load bus and then sends it to the corresponding customer aggregator. In the lower level, every customer aggregator exploits available controllable demands like heat pump (HP), electric vehicle (EV) to minimize the nodal daily cost based on the barrier cost calculated in DSO. Numerical tests verify the promising convergence of this decomposition algorithm and show a reduction of the total cost in DN.

关键词： distribution network network reconfiguration demand response decomposition algorithm

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基于Ecopath评估蟹-稻复合生态系统营养结构和能量流动

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生态学报 2020年第14期40卷 4852-4862页

作者：汪倩胡庚东宋超李丹丹郑尧裘丽萍范立明孟顺龙陈家长邴旭文中国水产科学研究院淡水渔业研究中心无锡214081 中国水产科学研究院内陆渔业生态环境和资源重点开放实验室无锡214081 农业农村部长江下游渔业生态环境监测中心无锡214081 南京农业大学无锡渔业学院无锡214081

蟹-稻综合种养模式为我国水产养殖以及农业从单一经营转变为复合经营提供了一种新模式,为了促进蟹-稻共作模式的推广和普及,应用Ecopath生态通道模型对该系统进行了营养结构和能量流动的分析。研究结果表明蟹-稻共作生态系统的最高营养... 详细信息

蟹-稻综合种养模式为我国水产养殖以及农业从单一经营转变为复合经营提供了一种新模式,为了促进蟹-稻共作模式的推广和普及,应用Ecopath生态通道模型对该系统进行了营养结构和能量流动的分析。研究结果表明蟹-稻共作生态系统的最高营养级(3.140)高于稻田单作系统的最高营养级(2.676),生态系统的能量流动主要有两条途径,即以沉水植物和其他维管束植物为起点的牧食链及以碎屑为起点的腐质链;蟹-稻共作农田生态系统在6月和10月的Finn′s循环指数(Finn′s cycling index,FCI,0.55和1)和Finn′s循环平均能流路径长度(Finn′s cycling mean path length,FCL,2.066和2.077),稻田单作农田生态系统在6月和10月的FCI(1.14和0.81)和FCL(2.089和2.137),说明蟹-稻共作模式可以形成一个稳定的生态系统,中华绒螯蟹的加入可以促进该完整的生态系统的能量流动,且随着螃蟹的成长,系统成熟度有所提高,只是较单作系统的成熟度低,共作系统有更大的成熟空间;蟹-稻共作农田生态系统在6月和10月的连接指数(Connectance index,CI)分别为0.135和0.135,稻田单作系统在6月和10月的CI为0.188和0.161,共作系统的各功能组之间的连接强度较单作系统弱,稳定程度相对较低,蟹-稻共作农田生态系统的初级生产力显著高于单作农田生态系统,且蟹-稻共作农田生态系统的发育良好。

关键词：生态通道模型蟹-稻复合生态系统营养结构能量流动

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琼脂糖凝胶电泳法测定人血白蛋白及人免疫球蛋白类制品的纯度

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中国药品标准 2020年第2期21卷 159-166页

作者：穆矛刘冰滢陈宇堃焦旭雯梁蔚阳广东省药品检验所广州510663

目的:建立琼脂糖凝胶电泳法测定人血白蛋白及人免疫球蛋白类制品的纯度。方法:采用Helena公司的SPIFE Touch型自动电泳仪及配套的试剂盒,对琼脂糖凝胶电泳法的方法学进行验证。以供试品浓度5%,对33批人血白蛋白样品以及16批人免疫球蛋... 详细信息

目的:建立琼脂糖凝胶电泳法测定人血白蛋白及人免疫球蛋白类制品的纯度。方法:采用Helena公司的SPIFE Touch型自动电泳仪及配套的试剂盒,对琼脂糖凝胶电泳法的方法学进行验证。以供试品浓度5%,对33批人血白蛋白样品以及16批人免疫球蛋白类制品进行纯度测定,并与《中国药典》2015版规定方法(醋酸纤维素薄膜法)的测定结果进行比较。结果:该方法检测正常人血清中白蛋白与免疫球蛋白的RSD分别为1.15%和1.45%,最佳供试品蛋白浓度为5%,仪器通道间差异的RSD小于0.4%,重复性的RSD均小于0.5%。对比两种方法的检测结果,人血白蛋白的两法结果趋势一致且高度相关(Pearson′s r=0.88),但琼脂糖凝胶电泳法比醋酸纤维素薄膜法测定结果低1%~4%;人免疫球蛋白类制品的检测结果两种方法基本一致(Pearson′s r=0.66,两法差值小于1%)。结论:该法经方法学验证,可作为人血白蛋白和人免疫球蛋白类制品的纯度测定替代方法。

关键词：琼脂糖凝胶电泳人血白蛋白人免疫球蛋白纯度

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不同浸种处理对美丽决明和绒果决明种子萌发的效果研究

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种子 2019年第5期38卷 70-73页

作者：陈莹孙苗苗钟丽媚李许文代色平广州市林业和园林科学研究院

以美丽决明和绒果决明为研究材料,观察种子外部形态,测定种子长度、宽度、厚度、千粒重和硬实率,并研究温水浸种(处理温度为20,40,60℃和80℃)、100℃热水浸种(处理时间为5,10 min和20 min)及98%浓硫酸浸种(处理时间为2,5,10,20 min和30... 详细信息

以美丽决明和绒果决明为研究材料,观察种子外部形态,测定种子长度、宽度、厚度、千粒重和硬实率,并研究温水浸种(处理温度为20,40,60℃和80℃)、100℃热水浸种(处理时间为5,10 min和20 min)及98%浓硫酸浸种(处理时间为2,5,10,20 min和30 min)对2种决明属硬实种子萌发的影响。结果表明:美丽决明种子长度(5.44±0.24)mm,宽度(4.39±0.24)mm,厚度(1.49±0.14)mm,千粒重为(2.66±0.07)g,硬实率85.00%;绒果决明种子长度(8.38±0.54)mm,宽度(5.49±0.46)mm,厚度(1.79±0.65)mm,千粒重(5.54±0.03)g,硬实率82.50%。温水浸种处理和浓硫酸浸种处理对美丽决明和绒果决明2种硬实种子的萌芽都表现出明显的促进作用,但不可采用100℃的热水破除种子硬实。本试验中,采用98%浓硫酸浸种美丽决明种子20 min和采用98%浓硫酸浸种绒果决明种子30 min,是打破种子硬实最佳的方法,发芽率分别达到30.00%和90.00%,比对照分别提高了1.61倍和3.75倍。

关键词：苏木科美丽决明绒果决明种子硬实萌发

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Non-O1 Vibrio cholerae pathogen from Cyprinus carpio and control with anti-non-O1 V. cholerae egg yolk powder (IgY)

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AQUACULTURE 2017年 479卷 69-74页

作者： Gao, Xiaojian chen, Nan Zhang, Yue Zhang, Xiaojun Bing, xuwen Yangzhou Univ Coll Anim Sci & Technol Yangzhou Jiangsu Peoples R China Chinese Acad Fishery Sci Minist Agr Freshwater Fisheries Res Ctr Key Lab Freshwater Fisheries & Germplasm Utilizat Wuxi Peoples R China

Mass mortality in cultured common carp from a farm of Ganyu county, Jiangsu province, P. R. China was reported. The two dominated isolates named L1 and L2 from diseased common carps were confirmed as non-O1 Vibrio cholerae by biochemical characteristics and molecular tools. Virulence genes (ompW, hlyA, acfA, toxR, ace and rtxC genes) were found to be present in the isolates by PCR, which further confirmed pathogenicity of the isolates. The LD50 value of the isolate L1 to the common carp was 1.1 x 10(6) CFU/mL. In addition, the specific egg yolk powder (IgY) conferred significant protection of common carp against non-O1 V. cholerae. The specific IgY also exhibited significant bacteriolytic effects on the non-O1 V. cholerae pathogen. The results showed that the specific IgY should be an effective implemented feed additive for the biocontrol of non-O1 V. cholerae infection in aquaculture.

关键词： Cyprinus carpio Non-O1 Vibrio cholerae Virulence gene Egg yolk powder (IgY)

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Adhesive Coacervates: Adhesive Coacervates Driven by Hydrogen-Bonding Interaction (Small 43/2020)

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Small 2020年第43期16卷

作者： Qiongyao Peng Jingsi chen Zicheng Zeng Tao Wang Li Xiang xuwen Peng Jifang Liu Hongbo Zeng Department of Chemical and Materials Engineering University of Alberta Edmonton Alberta T6G 1H9 Canada The Fifth Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong 510700 China

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Provenance of uranium in a sediment core from a natural reservoir, South China: Application of Pb stable isotope analysis

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CHEMOSPHERE 2018年 193卷 1172-1180页

作者： Liu, Juan Luo, xuwen Wang, Jin Xiao, Tangfu Yin, Meiling Belshaw, Nick Stanley Lippold, Holger Kong, Lingjun Xiao, Enzhong Bao, Zhi'an Li, Nuo chen, Yongheng Linghu, Wensheng Guangzhou Univ Minist Educ Innovat Ctr Guangzhou 510006 Guangdong Peoples R China Guangzhou Univ Minist Educ Key Lab Waters Safety & Protect Pearl River Delta Guangzhou 510006 Guangdong Peoples R China Univ Oxford Dept Earth Sci Oxford OX1 3AN England Helmholtz Zentrum Dresden Rossendorf eV HZDR Inst Ressourcenokol D-04318 Leipzig Germany Northwest Univ Xian Dept Geol State Key Lab Continental Dynam Xian 710069 Shaanxi Peoples R China Shaoxing Univ Coll Life Sci Coll Chem & Chem Engn Shaoxing 312000 Peoples R China

As part of ongoing environmental investigations of U mining impacts, forty-two sediment samples of a nearly-half-meter-long sediment core retrieved from a natural reservoir near an active uranium (U) mining site, South China were analyzed to quantify the extent of U release and identify U release mechanism within the riverine catchment. Enrichment levels of U was dispersed not only in the surface sediments but also in deep sediments across the depth profile. Further analysis by SEM-EDS and XRD indicated that U partitioning in the depth profile was possibly controlled by complicated interplay of leaching and precipitation cycles of U-bearing minerals. Even with the relative complexity of U dispersal processes within the catchment, the Pb isotopic fingerprinting techniques allowed quantification of source inputs of the sediments by using a binary mixing model. The results revealed that along the depth profile, only 6%-50% of the sediment material is anthropogenically derived from the U ore tailing, with the other predominant proportions originated from geogenically natural weathering of granitic bedrocks. This study highlights the use of Pb isotopes as a powerful tool for quantitatively fingerprinting the sources of U dispersal in the sediment core, and natural-occurring U contamination that may become a hidden geoenvironmental health hazard in this area. (C) 2017 Elsevier Ltd. All rights reserved.

关键词： Uranium migration Pb isotopes Source apportionment Binary mixing Radiogenic

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