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中国环境监测 2021年第1期37卷 1-10页

作者：朱冰清姜晟蔡琨张小琼李旭文徐东炯刘佳江苏省环境监测中心江苏南京210019 江苏省常州环境监测中心江苏省环境保护水环境生物监测重点实验室江苏常州213001 中国环境科学研究院北京100012

生物监测可以系统反映污染物对生物生长的影响及其在生物体内的转化和迁移,在水环境监测与生态健康管理中的重要性日益突出。伴随着工业化的快速发展,中国水环境污染问题依然严峻,工业废水治理和排放问题仍旧突出。为进一步保障工业废... 详细信息

生物监测可以系统反映污染物对生物生长的影响及其在生物体内的转化和迁移,在水环境监测与生态健康管理中的重要性日益突出。伴随着工业化的快速发展,中国水环境污染问题依然严峻,工业废水治理和排放问题仍旧突出。为进一步保障工业废水出水及受纳水体水质安全,迫切需要在工业废水监测中引入生物监测技术。对传统微生物群落监测法、水生生物毒性监测法和基于分子生物学技术的微生群落监测法等在工业废水监测领域的研究及应用进行了综述,并对存在的问题进行了总结。建议后续进一步完善工业废水生物监测体系时,参考国外成熟经验,制定出符合国内需求及特征的工业废水生物监测方法与评价标准,以便更好地掌握工业区整体环境健康及污染状况。

关键词：生物监测工业废水环境监测

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Disrupting Tumor Lactate Homeostasis to Sensitize Chemo-Immunotherapy Using a Glucose-Disguised Lactate Interceptor

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ACS nano 2025年第23期19卷 2025 Jun 5页

作者： xuwen Li Shuo Geng Qinjun Chen Boyu Su Haolin Song Jingyi Zhou Ru jia Hongrui Fan Chufeng Li Yu Wang Zonghua Tian Tao Sun Chen jiang Department of Pharmaceutics School of Pharmacy Fudan University Shanghai 201203 China. Key Laboratory of Smart Drug Delivery Ministry of Education Fudan University Shanghai 201203 China. State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan University Shanghai 201203 China. Department of Digestive Diseases National Regional Medical Center Binhai Campus of the First Affiliated Hospital Fujian Medical University Fuzhou 350212 China.

Aberrantly elevated lactate flux in tumors is increasingly recognized as a key driver of metabolic symbiosis, immunosuppression, and, ultimately, immunogenic chemotherapy resistance. Here, we propose a precise lactate homeostasis modulation strategy that selectively intercepts intracellular lactate molecules in highly glycolytic tumor cells. Targeting monocarboxylate transporter 4 (MCT4), a key lactate efflux transporter overexpressed in tumor cells, we developed a glucose-disguised delivery system for precise transport of regulatory molecules into glycolysis-dependent tumor cells. By modulating lactate-mediated crosstalk between heterogeneous tumor subpopulations (glycolysis-dependent and lactate-consuming cells) and immune cells, this strategy effectively disrupts lactate-driven metabolic cooperation within the tumor niche, which may contribute to overcoming lactate-associated resistance to chemo-immunotherapy.

关键词： chemo-immunotherapy sensitization immune activation lactate metabolic regulation metabolic symbiosis tumor microenvironment

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冬凌草甲素抗炎机制研究进展

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中国野生植物资源 2023年第2期42卷 88-93页

作者：李彩芸陈旭雯岳飞燕 Sani Ibrahim 张艳岳嘉朱向东樊景春甘肃中医药大学公共卫生学院甘肃兰州730101 甘肃中医药大学基础医学院甘肃兰州730101 宁夏医科大学中医学院宁夏银川750004 甘肃中医药大学教学实验实训中心甘肃兰州730101 甘肃中医药大学循证医学中心甘肃兰州730101 西北环境与营养相关疾病的中医药防控协同创新中心甘肃兰州730101

冬凌草为唇形科香茶属植物,性微寒,有活血止痛、解热降噪和清热解毒的功效,近年来研究发现其主要活性成分冬凌草甲素具有降压、抗氧化、抗菌、抗肿瘤、抗凋亡、增强机体免疫等多种药理作用。基于现代医学研究,从核因子κB(NF-κB)、丝... 详细信息

冬凌草为唇形科香茶属植物,性微寒,有活血止痛、解热降噪和清热解毒的功效,近年来研究发现其主要活性成分冬凌草甲素具有降压、抗氧化、抗菌、抗肿瘤、抗凋亡、增强机体免疫等多种药理作用。基于现代医学研究,从核因子κB(NF-κB)、丝裂原活化蛋白激酶(MAPK)和NOD样受体热蛋白结构域相关蛋白3(NLRP3)等信号通路方面,对冬凌草甲素在呼吸系统炎症、消化系统炎症、神经性炎症、肾损伤性炎症、血管炎等疾病抗炎作用机制的研究进展进行综述,为研究与开发以冬凌草甲素为原料的抗炎新药提供参考。

关键词：冬凌草甲素炎症 NF-κB MAPK NLRP3

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Oral immunization of mice with a probiotic Lactobacillus casei constitutively expressing the α-toxoid induces protective immunity against Clostridium perfringens α-toxin

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VIRULENCE 2019年第1期10卷 166-179页

作者： Gao, xuwen Ma, Yingying Wang, Zhuo Bai, Jing jia, Shuo Feng, Baohua jiang, Yanping Cui, Wen Tang, Lijie Li, Yijing Wang, Li Xu, Yigang Northeast Agr Univ Coll Vet Med Harbin Heilongjiang Peoples R China China Minist Agr Key Lab Anim Pathogen Biol Northeastern Sci Inspect Stn Harbin Heilongjiang Peoples R China Northeast Agr Univ Heilongjiang Key Lab Anim Dis Control & Pharmaceu Harbin Heilongjiang Peoples R China

Clostridium perfringens alpha-toxin is one of the major virulence factors during C. perfringens infection, causing hemolysis of erythrocytes in various species. Here, genetically engineered Lactobacillus casei (pPG-alpha/L. casei 393) constitutively expressing the toxoid of C. perfringens alpha-toxin was generated and its immunogenicity in mice for induction of protective immunity against the alpha-toxin was evaluated via oral immunization. The alpha-toxoid was constitutively expressed by pPG-alpha/L. casei 393 without a specific inducer, as confirmed by western blotting, laser confocal microscopy, and flow cytometry. In an experiment on BALB/c mice to evaluate the oral immunogenicity of pPG-alpha/L. casei 393, significant levels of a specific secretory IgA (sIgA) antibody in the intestinal mucus and feces and an IgG antibody in the serum of the probiotic vaccine group were detected after booster immunization (p < 0.05) as compared with the pPG/L. casei 393 and PBS control groups. These antibodies effectively neutralized C. perfringens natural alpha-toxin. Moreover, significantly higher levels of cytokines IL-2, IL-4, IL-10, IL-12, IL-17, and interferon (IFN) gamma in the serum and increased proliferation of spleen lymphocytes obtained from mice orally immunized with pPG-alpha/L. casei 393 were detected. With a commercial C. perfringens type A inactivated vaccine as a control, immune protection provided by the probiotic vaccine against C. perfringens alpha-toxin was evaluated, and 90% and 80% protection rates were observed, respectively. Therefore, strain pPG-alpha/L. casei 393 effectively elicited mucosal, humoral, and cellular immunity, suggesting that pPG-alpha/L. casei 393 is a promising candidate for development of a vaccine against C. perfringens alpha-toxin.

关键词： Clostridium perfringens alpha-toxoid genetically engineered Lactobacillus casei oral immunization

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Epidemiological investigation of feline infectious peritonitis in cats living in Harbin, Northeast China from 2017 to 2019 using a combination of an EvaGreen-based real-time RT-PCR and serum chemistry assays

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MOLECULAR AND CELLULAR PROBES 2020年第0期49卷 101495-000页

作者： Guan, Xueting Li, Hua Han, Meijing jia, Shuo Feng, Baohua Gao, xuwen Wang, Zhuo jiang, Yanping Cui, Wen Wang, Li Xu, Yigang Northeast Agr Univ Heilongjiang Key Lab Anim Dis Control & Pharmaceu Harbin Peoples R China Minist Agr China Key Lab Anim Pathogen Biol Northeast Sci Inspect Stn Harbin Peoples R China

Feline infectious peritonitis (FIP) is caused by the FIP virus (FIPV), a highly virulent mutant form of feline coronavirus (FCoV). This disease is one of the most important infectious diseases in cats, and it is associated with high mortality, particularly among younger cats. In this study, we isolated a wild-type FIPV HRB-17 epidemic strain from the blood sample of household pet cat exhibiting the characteristic wet-form FIP symptoms, which has been confirmed further by animal infection. Further, we developed an EvaGreen-based real-time RT-PCR assay for the accurate detection of FCoV based on the amplification of the highly conserved FIPV N gene. Then, using a combination of the real-time RT-PCR approach and a serum chemistry assay, we performed an epidemiological survey of FIPV infection in cats living in Harbin City, Northeast China. The results indicated that the EvaGreen-based real-time RT-PCR assay can be used for screening FCoV infection in the affected cats at an analytical detection limit of 8.2 x 10(1) viral genome copies/A, but could not effectively distinguish FIPVs from FECVs. Additionally, the results of the epidemiological survey investigating feline blood samples (n = 1523) collected between July 2017 to July 2019 revealed an FIPV prevalence of approximately 12% (189/1523). Maybe, the prevalence would be less than 12% due to the real-time RT-PCR assay could not accurately differentiate FIPV and FECV. Nevertheless, it still highlighted the severity of the FIP epidemic in cats and reiterated the urgent need to develop effective anti-FIP therapeutic agents and anti-FIPV vaccines. As pet cats are household animals, risk communication and continuous region-extended surveillance cat programs are recommended.

关键词： Feline infectious peritonitis virus N gene EvaGreen-based real-time 12T-PCR Hyperglobulinemia Epidemiological survey

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大蒜提取物对小鼠肠道菌群调节作用研究

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新疆医科大学学报 2021年第10期44卷 1165-1170页

作者：贾世栋杨浩宋百灵张春子毛旭文新疆医科大学药学院乌鲁木齐830011 新疆医科大学第六附属医院药学部乌鲁木齐830011 埃乐欣药业有限公司乌鲁木齐830011 天然药物活性组分与释药技术重点实验室乌鲁木齐830011

目的探究蒜氨酸、大蒜辣素、大蒜粉对小鼠肠道菌群的调节作用。方法将100只小鼠分为:空白对照组(灌胃蒸馏水,灌胃剂量0.1 mL/10 g体质量),蒜氨酸低(0.0043 mg/g体质量)、中(0.0086 mg/g体质量)、高(0.0172 mg/g体质量)剂量组,大蒜辣素低... 详细信息

目的探究蒜氨酸、大蒜辣素、大蒜粉对小鼠肠道菌群的调节作用。方法将100只小鼠分为:空白对照组(灌胃蒸馏水,灌胃剂量0.1 mL/10 g体质量),蒜氨酸低(0.0043 mg/g体质量)、中(0.0086 mg/g体质量)、高(0.0172 mg/g体质量)剂量组,大蒜辣素低(0.0019 mg/g体质量)、中(0.0039 mg/g体质量)、高(0.0078 mg/g体质量)剂量组,大蒜粉低(0.1108 mg/g体质量)、中(0.2216 mg/g体质量)、高(0.4432 mg/g体质量)剂量组。灌胃30 d后,采用菌落计数法检测实验前后粪便中乳杆菌、双歧杆菌、肠杆菌、肠球菌、产气荚膜梭菌数量变化。K-B纸片扩散法检测蒜氨酸、大蒜辣素、大蒜粉对5种菌的抑菌作用。结果与对照组比较,给药30 d后蒜氨酸、大蒜辣素、大蒜粉组乳杆菌、双歧杆菌数量增加,产气荚膜梭菌数量减少,差异有统计学意义(P<0.01)。体外抑菌试验表明,肠球菌、产气荚膜梭菌对蒜氨酸均低敏;乳杆菌、双歧杆菌、肠杆菌对蒜氨酸均不敏。大蒜辣素、大蒜粉对肠球菌、肠杆菌、乳杆菌、产气荚膜梭菌具有抑菌作用,并呈现剂量依赖性;对双歧杆菌对均不敏。结论蒜氨酸、大蒜辣素、大蒜粉对肠道菌群具有有益的调节作用,是一种良好的天然有益于肠道菌群的食品。

关键词：蒜氨酸大蒜辣素大蒜粉肠道菌群益生

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DFT Study of Oxygenated Organic Pollutants Catalyzed by Molybdenum Oxides: Comparison of Reaction Mechanisms of MoO_x + HCHO(x = 1, 2, 3)

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Chinese Journal of Structural Chemistry 2019年第8期38卷 1229-1240页

作者： GAO Xiao-Zhen LIANG Yan YANG Jing MENG Xiang-Jun YANG Xiao-Chun jia Jing-Xian xuwen-Guo Yangquan Municipal Key Laboratory of Quantum Manipulation Shanxi Institute of TechnologyYangquan045000China Department of Chemistry Tangshan Normal CollegeTangshan063000China Institute for Physical Chemistry Beijing Institute of TechnologyBeijing100081China

The reaction mechanisms between formaldehyde and MoOx(x = 1, 2, 3) have been studied thoroughly in this paper. Five reaction pathways were found in three reactions(reactions Ⅰ to Ⅲ) through studying the mechanisms of MoOx(x = 1, 2, 3) catalyzing formaldehyde. Different products were obtained from three reactions. Of all three reactions, the barrier energy of Route ⅡA is the lowest(4.70 kcal/mol), which means in MoOx(x = 1, 2, 3), MoO2 has the best catalytic effect. Compared with other similar non-toxic treatments of formaldehyde, our barrier energy is the lowest. In this research, there was no good leaving group of the compound, so the mechanisms are addition reaction. We speculate that there must be an addition reaction to the more complex reactions to molybdenum oxides and aldehydes. As a chemical reagent for removing formaldehyde, it only absorbs formaldehyde and does not emit other toxic substances outward. Molybdenum oxides retain its original structures of the final products, which means it has excellent stability in the reaction of MoOx(x = 1, 2, 3) + HCHO. The mechanisms of all three reactions are addition reactions, but they are entirely different. As the number of oxygen atoms increases, the reaction mechanisms become simpler.

关键词： reaction mechanism formaldehyde elimination molybdenum oxides addition reaction barrier energy

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Determination of IgG by electron spin resonance spectroscopy using Fe₃O₄ nanoparticles as probe

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MICROCHEMICAL JOURNAL 2018年 141卷 444-450页

作者： Tian, Sizhu jiang, jia Zang, Shuang Wang, Kun Yu, Yong Li, xuwen Zhang, Hanqi Yu, Aimin Zhang, Ziwei Jilin Univ Coll Chem Qianjin St 2699 Changchun 130012 Jilin Peoples R China Jilin Univ Coll Instrumentat & Elect Engn West Minzhu St 938 Changchun 130061 Jilin Peoples R China

A novel electron spin resonance (ESR) spectroscopy method for determination of rabbit IgG was presented. Anti rabbit IgG antibody was bound to Fe3O4 nanoparticles (FeNPs). The FeNPs were used as probe to give ESR signal. After the optimization of the assay, a molar ratio 500:1 of antibody to FeNPs was selected. The sepharose beads were used as solid phase and used to separate the analyte from the sample solution. Two typical immunoassay formats, sandwich and competitive formats, were applied to the determination of IgG. The analytical sensitivities obtained by sandwich and competitive formats were 0.13 mu g mL(-1) and 0.56 mu g mL(-1), respectively. The two formats were applied to the determination of the rabbit IgG in rabbit serum. The diluted sample was directly analyzed and other additional sample treatment was not required. The analytical results of the spiked samples indicated that the recoveries of the analytes were acceptable. Furthermore, the sample preparation time used in this method is shorter than most existing immunoassay methods.

关键词： Electron spin resonance Sandwich Competitive Fe3O4 nanoparticles Sepharose beads Rabbit serum

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油藏型储气库高效除黑油技术研究与实践

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天然气与石油 2021年第3期39卷 10-17页

作者：刘贤明王铁军贾雪松王林峰丛延刚王绪文寇鹏中国石油北京项目管理公司天津设计院天津300457 大港油田天津储气库分公司天津300270 中国石油吉林油田公司天然气部吉林松原138000

在采气期,油藏型储气库采出的天然气会携带少量黑油进入下游低温装置,导致黑油凝固析出,造成设备、管线堵塞,影响装置安全运行。为解决油藏型储气库采出天然气携带黑油的问题,避免析出黑油影响储气库采气装置的正常运行,经分析研究设置... 详细信息

在采气期,油藏型储气库采出的天然气会携带少量黑油进入下游低温装置,导致黑油凝固析出,造成设备、管线堵塞,影响装置安全运行。为解决油藏型储气库采出天然气携带黑油的问题,避免析出黑油影响储气库采气装置的正常运行,经分析研究设置旋流分离器+过滤分离器两级过滤除黑油流程,从天然气中过滤出99.9%以上直径大于1μm的液滴,减少天然气携带黑油进入后续低温处理装置,提高了冬季调峰、储气库供气的安全性及可靠性。两级过滤除黑油技术在中国首座油藏型储气库中已成功应用,为后续同类型储气库的建设提供了经验借鉴。同时,根据除黑油过滤机理,研发出一体化精细除黑油分离器,此分离器能高效地提高油藏型储气库除黑油的效果,建议将分离器设置在采气装置空冷器后。一体化精细除黑油分离器的设置可为后续同类型储气库设计提供指导。

关键词：油藏型储气库除黑油过滤器选型配置

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深层平板载荷试验变形模量计算新方法

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山西建筑 2020年第8期46卷 1-4页

作者：尚许雯尚银生贾迎泽杨进堂孙存珠山西省交通规划勘察设计院有限公司山西太原030012 山西省勘察设计研究院有限公司山西太原030013 山西省建筑科学研究院有限公司山西太原030001

根据Mindlin课题解析解,结合深层平板载荷试验的尺寸确定及试验深度等技术要求,提出一个新的计算变形模量公式。当μ=0.00~0.50;z=5.0 m,10.0 m及趋于无穷大时(工程意义上或d z,d/22z),其相应于修正系数的部分,在不大于0.500的范围内,... 详细信息

根据Mindlin课题解析解,结合深层平板载荷试验的尺寸确定及试验深度等技术要求,提出一个新的计算变形模量公式。当μ=0.00~0.50;z=5.0 m,10.0 m及趋于无穷大时(工程意义上或d z,d/22z),其相应于修正系数的部分,在不大于0.500的范围内,随土类的泊松比增大而增大,其值介于0.375~0.500;高氏修正系数在不小于0.500的范围内,随土类泊松比的增加而增加,其值介于0.500~0.902;顾氏修正系数随试验深度的增加而减小,其值介于0.500~0.555。并指出现行《岩规》推荐的深层平板载荷试验变形模量计算方法建立过程不够严谨,不能满足有关技术要求。

关键词：深层平板载荷试验变形模量 Mindlin解析解泊松比修正系数

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