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生物工程学报 2017年第2期33卷 217-227页

作者：侯立婷陈瑾乔绪稳于晓明侯继波郑其升李槿年安徽农业大学动物科技学院安徽合肥230036 国家兽用生物制品工程技术研究中心江苏南京210014 江苏省动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009

验证基于革兰氏阳性增强基质(Gram-positive enhancer matrix,GEM)展示口蹄疫病毒细菌样颗粒(Bacteria-like particles,BLP)疫苗的可行性。按照大肠杆菌偏好性密码子优化合成基于猪口蹄疫病毒Mya98株序列的3种抗原基因设计,并将其插入... 详细信息

验证基于革兰氏阳性增强基质(Gram-positive enhancer matrix,GEM)展示口蹄疫病毒细菌样颗粒(Bacteria-like particles,BLP)疫苗的可行性。按照大肠杆菌偏好性密码子优化合成基于猪口蹄疫病毒Mya98株序列的3种抗原基因设计,并将其插入到含有锚钩蛋白基因的原核表达载体p QZ-PA,鉴定阳性后转入Escherichia coli BL21,进行诱导表达。利用SDS-PAGE与Western blotting对目的基因表达及产物的可溶性进行分析。利用GEM颗粒纯化目的蛋白,制备细菌样颗粒疫苗抗原;利用BCA试剂盒测定重组蛋白的浓度,将重组蛋白与白油佐剂乳化,制备疫苗,免疫5周龄小鼠,同时设商品化多肽苗对照与空白对照,免疫后不同时间采集试验小鼠血清,利用口蹄疫病毒多肽EliSA抗体检测试剂盒和O型口蹄疫抗体液相阻断酶联免疫(Enzyme-linked immunosorbent assay,EliSA)检测试剂盒检测免疫小鼠血清的抗体水平;利用噻唑蓝比色法(Methylthiazolyldiphenyl-tetrazolium bromide,MTT)测定淋巴细胞增殖情况;利用荧光定量PCR方法检测相关细胞因子表达,评价细胞免疫水平。SDS-PAGE结果表明,设计在大肠杆菌中的3种口蹄疫病毒抗原基因均以可溶形式获得高效表达;Western blotting结果显示,表达的重组蛋白能够与口蹄疫病毒阳性血清发生反应,利用GEM颗粒能够实现重组蛋白的一步离心纯化,制备BLP疫苗抗原;免疫试验结果表明,设计的重组抗原B(T1BT2)4B不但能够刺激免疫小鼠产生更高水平的多肽特异性EliSA抗体与口蹄疫特异性液相阻断抗体,而且产生了更高水平的脾淋巴细胞增殖及Th1型的细胞因子分泌。初步实验结果表明,本研究制备的BLP疫苗GEM-B(T1BT2)4B具有良好的免疫原性,为研究口蹄疫病毒基因工程亚单位疫苗开辟了一条新的思路。

关键词：口蹄疫病毒 VP1蛋白可溶性表达细菌样颗粒疫苗免疫原性

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陆地棉GhSAD2基因表达载体的构建、转化及抗寒性初步分析

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华北农学报 2017年第6期32卷 60-66页

作者：蔡曼李卫华柳延涛余渝孔宪辉王娟王旭文刘丽新疆农垦科学院棉花研究所农业部西北内陆区棉花生物学与遗传育种重点实验室国家棉花改良中心新疆生产建设兵团分中心新疆石河子832000 石河子大学新疆兵团绿洲生态农业重点实验室新疆石河子832003 新疆农垦科学院作物研究所新疆石河子832000

为研究Δ9-硬脂酰-ACP脱氢酶基因的功能及其与植物抗寒性的相关性,以陆地棉GhSAD2基因(KX197920)为目的基因,构建植物表达载体pBI121-GhSAD2,将该表达载体通过电击法导入根癌农杆菌GV3101中,采用根癌农杆菌介导的叶盘法转化烟草。通过PC... 详细信息

为研究Δ9-硬脂酰-ACP脱氢酶基因的功能及其与植物抗寒性的相关性,以陆地棉GhSAD2基因(KX197920)为目的基因,构建植物表达载体pBI121-GhSAD2,将该表达载体通过电击法导入根癌农杆菌GV3101中,采用根癌农杆菌介导的叶盘法转化烟草。通过PCR和GUS活性染色鉴定外源GhSAD2基因是否导入并整合到烟草基因组上转录表达。将转pBI121-GhSAD2型和野生型烟草置于4℃下处理48 h后,检测其电解质渗漏率并观察其表型。研究结果证明目的基因GhSAD2已整合到烟草及棉花的基因组中,共获得转基因植株9株,转化率为75%。陆地棉GhSAD2基因能够显著增强非低温驯化植物烟草的抗寒性,从而提高植物细胞的低温防御能力,为进一步探究该基因的功能鉴定了基础。

关键词：陆地棉 GhSAD2基因表达载体构建遗传转化

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神华上湾煤恒温阶段直接液化反应动力学

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化工学报 2017年第4期68卷 1398-1406页

作者：单贤根李克健章序文王洪学曹雪萍江洪波翁惠新华东理工大学石油加工研究所上海200237 中国神华煤制油化工有限公司上海研究院上海201108

为考察神华上湾煤的直接液化性能及反应动力学,以加氢蒽油-洗油混合油作为溶剂、负载型FeOOH作为催化剂,在0.01 t·d^(-1)煤直接液化连续实验装置上考察了不同反应温度(435~465℃)、不同停留时间(7~110 min)下液化产品组成的演变规... 详细信息

为考察神华上湾煤的直接液化性能及反应动力学,以加氢蒽油-洗油混合油作为溶剂、负载型FeOOH作为催化剂,在0.01 t·d^(-1)煤直接液化连续实验装置上考察了不同反应温度(435~465℃)、不同停留时间(7~110 min)下液化产品组成的演变规律。研究发现,随着煤的裂解及加氢反应的进行,煤及沥青类物质(PAA)收率不断减小,重质液化产物逐步向轻质液化产物转化。当反应温度为455℃、停留时间为90 min时,煤转化率为90.41%(质量分数),油收率为61.28%(质量分数)。随着反应条件进一步苛刻,油收率下降。基于上湾煤直接液化反应特性及其产物收率变化规律建立了11集总煤直接液化反应动力学候选模型,以BFGS优化算法对实验数据搜索、选优,确定了动力学模型参数。检验结果表明所建立的动力学模型可用于恒温阶段直接液化行为的模拟计算。

关键词：煤直接液化反应动力学集总模拟模型

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Biomedical Network Regulated by Acanthopanacis Cortex (Wu-Jia-Pi) for Ossification 12

Biomedical Network Regulated by Acanthopanacis Cortex (Wu-Ji...

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12th International Conference on Natural Computation, Fuzzy Systems and Knowledge Discovery (ICNC-FSKD)

作者： Zhan, Junping Guo, Hongtao Yang, Yun Qi, Yutao li, Miaofeng He, xuwen Zheng, Guang Henan Univ TCM Affiliated Hosp 1 Zhengzhou 450000 Peoples R China Henan Univ TCM Affiliated Hosp 2 Zhengzhou 450002 Peoples R China Lanzhou Univ Sch Informat Sci & Engn Lanzhou 730000 Peoples R China

ISBN: (纸本)9781509040933

Acanthopanacis Cortex (AC) is a Chinese herbal medicine (Wu-Jia-Pi in PinYin) which is recognized with therapeutic effect of "strengthening bone and sinew", yet its regulating biological network is still obscure. In this study, started from AC's chemical compounds, their direct target proteins were filtered out. Then these targeted proteins were annotated with biological processes under the framework of Gene Ontology (GO) terms' hierarchical networks which were overrepresented and enriched with them with statistic significant p-value set to 0.05. In the biological process hierarchy networks, ossification formed a big one with bone formation associated biological processes. Further protein-protein interaction analysis with these targeted proteins and bone expressed ones formed a clear and concise protein-regulation network for ossification which should be responsible for the therapeutic effect of "strengthening bone and sinew". This study proposed a new approach of analyzing biological functions of a specified Chinese herbal medicine targeting certain therapeutic effect.

关键词： Acanthopanacis Cortex ossification Chinese herbal medicine protein-protein interaction biomedical network

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Tumor Suppressor Folliculin Regulates mTORC1 through Primary Cilia

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JOURNAL OF BIOLOGICAL CHEMISTRY 2016年第22期291卷 11689-11697页

作者： Zhong, Mingming Zhao, xuwen li, Jinmei Yuan, Wenjie Yan, Gonghong Tong, Mingming Guo, Shuguang Zhu, Yichao Jiang, Yong liu, Yongjian Jiang, Yu Southern Med Univ Minist Educ China State Key Lab Organ Failure ResDept Pathophysiol Key Lab Transcript & ProteGuangdong Prov Key Lab Guangzhou 510515 Guangdong Peoples R China Univ Pittsburgh Sch Med Dept Pharmacol & Chem Biol Pittsburgh PA 15261 USA Nanjing Med Univ Dept Physiol Nanjing 211166 Jiangsu Peoples R China

Folliculin (FLCN) is the tumor suppressor associated with Birt-Hogg-Dube (BHD) syndrome that predisposes patients to incident of hamartomas and cysts in multiple organs. Its inactivation causes deregulation in the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway. However, the underlying mechanism is poorly defined. In this study, we show that FLCN is a ciliary protein that functions through primary cilia to regulate mTORC1. In response to flow stress, FLCN associates with LKB1 and recruits the kinase to primary cilia for activation of AMPK resided at basal bodies, which causes mTORC1 down-regulation. In cells depleted of FLCN, LKB1 fails to accumulate in primary cilia and AMPK at the basal bodies remains inactive, thus nullifying the inhibitory effect of flow stress on mTORC1 activity. Our results demonstrate that FLCN is part of a flow sensory mechanism that regulates mTORC1 through primary cilia.

关键词： AMP-activated kinase (AMPK) mammalian target of rapamycin (mTOR) mTOR complex (mTORC) primary cilium tumor suppressor gene FLCN LKB1 folliculin

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Using gastric juice lncRNA-ABHD11-AS1 as a novel type of biomarker in the screening of gastric cancer

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TUMOR BIOLOGY 2016年第1期37卷 1183-1188页

作者： Yang, Yunben Shao, Yongfu Zhu, Mengying li, Qier Yang, Fang Lu, xuwen Xu, Chunjing Xiao, Bingxiu Sun, Yanke Guo, Junming Ningbo Univ Sch Med Dept Biochem & Mol Biol Ningbo 315211 Zhejiang Peoples R China Ningbo Univ Sch Med Zhejiang Key Lab Pathophysiol Ningbo 315211 Zhejiang Peoples R China Ningbo Univ Sch Med Affiliated Hosp Dept Gastroenterol Ningbo 315211 Zhejiang Peoples R China Zhenhai Middle Sch Ningbo Peoples R China

Long noncoding RNAs (lncRNAs) play vital roles in tumorigenesis. However, the diagnostic values of most lncRNAs are largely unknown. To investigate whether gastric juice lncRNA-ABHD11-AS1 can be a potential biomarker in the screening of gastric cancer, 173 tissue samples and 130 gastric juice from benign lesion, gastric dysplasia, gastric premalignant lesions, and gastric cancer were collected. ABHD11-AS1 levels were detected by reverse transcription-polymerase chain reaction. Then, the relationships between ABHD11-AS1 levels and clinicopathological factors of patients with gastric cancer were investigated. The results showed that ABHD11-AS1 levels in gastric cancer tissues were significantly higher than those in other tissues. Its levels in gastric juice from gastric cancer patients were not only significantly higher than those from cases of normal mucosa or minimal gastritis, atrophic gastritis, and gastric ulcers but also associated with gender, tumor size, tumor stage, Lauren type, and blood carcinoembryonic antigen (CEA) levels. More importantly, when using gastric juice ABHD11-AS1 as a marker, the positive detection rate of early gastric cancer patients was reached to 71.4 %. Thanks to the special origin of gastric juice, these results indicate that gastric juice ABHD11-AS1 may be a potential biomarker in the screening of gastric cancer.

关键词： ABHD11-AS1 Gastric cancer Gastric juice Gene diagnosis Long noncoding RNA Tumor marker

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Biological Functional Analysis of Chinese Herbal Medicines Against wind-cold-dampness Syndrome 12

Biological Functional Analysis of Chinese Herbal Medicines A...

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12th International Conference on Natural Computation, Fuzzy Systems and Knowledge Discovery (ICNC-FSKD)

作者： Zheng, Guang Zhang, Yanjing Zhang, He li, Miaofeng He, xuwen Qi, Yutao Zhan, Junping Guo, Hongtao Lanzhou Univ Sch Informat Sci & Engn Lanzhou 730000 Peoples R China Henan Univ TCM Affiliated Hosp 2 Zhengzhou 450002 Peoples R China Henan Univ TCM Affiliated Hosp 1 Zhengzhou 450000 Peoples R China

ISBN: (纸本)9781509040933

Syndrome wind-cold-dampness in traditional Chinese medicine (TCM) can be referred to diseases of osteoarthritis, rheumatoid arthritis, cervical spondylitis, and spondylitis and so on. Over thousand years TCM clinical explorations, this syndrome can be treated with a group of herbal medicines in TCM. However, their molecular level biological functions are still not clear. In this study, a set of algorithms e.g., hypergeometric distribution, Jaccard index, and recursive programming for hierarchy network were deployed in the context gene/protein functional similarity and enrichment analysis. The analysis was mainly focused on the associated herbal medicines, their chemical compounds, target proteins' similarity, and gene ontology terms' hierarchical network overrepresentation through the enrichment analysis of hypergeometric distribution with P-value set to 0.05. As a result, these associated herbs share low level (<0.5) of therapeutic similarities on target proteins, yet with common biological functions for TCM syndrome wind-cold-dampness e.g., signal transduction, transportation, chemical homeostatic and metabolic process.

关键词： bioinformatics wind-cold-dampness Chinese herbal medicine biological function hypergeometric distribution

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Research on Integrated Optimization Design Method for Diesel Engine Valve Train

Manufacturing Technology

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Manufacturing Technology 2016年第6期16卷 60-61页

作者： Zhou, Honggen Jing, xuwen He, Shuai li, Lei Institute of Advanced Manufacturing Technology Jiangsu University of Science and Technology Zhenjiang Jiangsu212003 China Engineering research department Saitama Institute of Technology Fukaya Saitama369-0293 Japan

In order to have a good performance of gas exchange in working process, a function of six order polynomial dynamic cam was established in this paper. The influence of the maximum ample factor to valve including the maximum positive and negative accelerations and the minimum curvature radius was analyzed by NLPQL. On this basis, multi-body dynamic analysis for diesel engine valve train was done, valve train dynamic model was established by ADAMS, multidisciplinary optimization process of cam profile and its model was put forward, and ISIGHT software was used to optimization and calculation. Finally, a set of different cam parameters and corresponding valve performance parameters was established, multipart integrated design and multidisciplinary optimization for diesel engine valve cam was realized. This paper provides a new method for the multicomponent integration design and multidisciplinary integration optimization for diesel engine valve train. © 2016. Published by Manufacturing Technology. All Rights Reserved.

关键词： Diesel engines

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混凝—气浮工艺预处理ABS树脂生产废水

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化工环保 2017年第1期37卷 55-61页

作者：罗梦何绪文林宏宇刘仪生吴佳兴李焱中国矿业大学(北京)化学与环境工程学院北京100083 中国石油吉林石化分公司合成树脂厂吉林吉林132021

采用混凝—气浮工艺对ABS树脂生产过程中的丁二烯聚合工段和乳液接枝工段混合废水进行预处理,优化了工艺条件。实验结果表明:最佳药剂组合为CaCl_2和阳离子型聚丙烯酰胺(FO4440SSH),最佳Ca Cl2投加量为75 mg/L,最佳FO4440SSH投加量为10 ... 详细信息

采用混凝—气浮工艺对ABS树脂生产过程中的丁二烯聚合工段和乳液接枝工段混合废水进行预处理,优化了工艺条件。实验结果表明:最佳药剂组合为CaCl_2和阳离子型聚丙烯酰胺(FO4440SSH),最佳Ca Cl2投加量为75 mg/L,最佳FO4440SSH投加量为10 mg/L,最佳废水p H范围为5~7;最优操作条件为以288 r/min的转速搅拌混凝1 min,再以72 r/min的转速搅拌絮凝20 min;混凝阶段的最佳G值为159.9 s^(-1)、GT值为9 594,絮凝阶段的最佳G值为24.5 s^(-1)、GT值为29 400;优化条件下,废水的浊度与COD去除率均可达98%以上。

关键词：丙烯腈一丁二烯一苯乙烯共聚物(ABS) 废水处理混凝絮凝气浮 G确莹

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新疆兵团机采棉发展现状与建议

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塔里木大学学报 2018年第3期30卷 57-61页

作者：余渝孔宪辉刘丽王旭文韩焕勇王方永陈兵刘景德新疆农垦科学院棉花研究所/农业部西北内陆区棉花生物学与遗传育种重点实验室/棉花遗传改良与高产栽培兵团重点实验室新疆石河子832000

棉花是兵团农业的支柱产业,兵团棉花产量占全国的四分之一以上。兵团机械化采棉经过二十年的发展已走在全国的前列,但机采棉生产仍然存在适宜机采棉花品种少、种质资源匮乏、机采棉种植技术有待于进一步研究完善、脱叶催熟剂使用亟待规... 详细信息

棉花是兵团农业的支柱产业,兵团棉花产量占全国的四分之一以上。兵团机械化采棉经过二十年的发展已走在全国的前列,但机采棉生产仍然存在适宜机采棉花品种少、种质资源匮乏、机采棉种植技术有待于进一步研究完善、脱叶催熟剂使用亟待规范、清理加工技术不优化、残膜污染严重等问题,导致近年机采棉质量下滑。今后需加强机采棉种质资源创新与新品种选育,完善机采棉配套栽培技术体系,改造生产机具、加工装备以及残膜污染治理技术。

关键词：机采棉品种种植加工发展现状

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