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Biocompatibility and Effectiveness Evaluation of a New Hemostatic Embolization Agent: Thrombin Loaded Alginate Calcium Microsphere

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BIOMED RESEARCH INTERNATIONAL 2017年第20170101期2017卷 1875258-1875258页

作者： Xuan, Fengqi Rong, Jingjing Liang, Ming Zhang, xuwen Sun, Jingyang zhao, Lijun Li, Yang Liu, Dan Li, Fei Wang, Xiaozeng Han, Yaling Gen Hosp Shenyang Mil Reg Dept Cardiol Shenyang 110016 Liaoning Peoples R China Dezhou Peoples Hosp Electrocardiogram Lab Dezhou 253000 Shandong Peoples R China Dezhou Peoples Hosp Dept Ophthalmol Dezhou 253000 Shandong Peoples R China

Background. Until now, there has been no ideal embolization agent for hemorrhage in interventional treatment. In this study, the thrombin was encapsulated in alginate calcium microsphere using electrostatic droplet technique to produce new embolization agent: thrombin loaded alginate calcium microspheres (TACMs). Objectives. The present work was to evaluate the biocompatibility and hemostatic efficiency of TACMs. Methods. Cell cytotoxicity, hemolysis, and superselective embolization of dog liver arteries were performed to investigate the biocompatibility of TACMs. To clarify the embolic effect of TACMs mixed thrombus in vivo, hepatic artery injury animal model of 6 beagles was established and transcatheter artery embolization for bleeding was performed. Results. Coculture with VECs revealed the noncytotoxicity of TACMs, and the hemolysis experiment was negligible. Moreover, the histological study of TACMs in liver blood vessel showed signs of a slight inflammatory reaction. The results of transcatheter application of TACMs mixed thrombus for bleeding showed that the blood flow was shut down completely after the TACMs mixed thrombus was delivered and the postprocedural survival rate of animal models at 12 weeks was 100%. Conclusions. With their good biocompatibility and superior hemostatic efficiency, TACMs might be a promising new hemostatic agent with a wide range of potential applications.

关键词： BLOOD analysis HEMORRHAGE treatment ALGINATES ANIMAL experimentation BIOLOGICAL assay BIOLOGICAL models BIOMEDICAL materials BLOOD circulation BODY temperature BODY weight CALCIUM CATHETERIZATION CELL culture CELL physiology DOGS ELECTROCARDIOGRAPHY ENZYMES EPITHELIAL cells HEMOLYSIS & hemolysins HEMOSTATICS HEPATIC artery INFLAMMATION LIVER MATERIALS testing PROBABILITY theory FINANCING of research SCANNING electron microscopy STATIC electricity THROMBIN TOXICITY testing THERAPEUTIC embolization TREATMENT effectiveness DATA analysis software DESCRIPTIVE statistics IN vitro studies KRUSKAL-Wallis Test IN vivo studies

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A Dopant Replacement-Driven Molten Salt Method toward the Synthesis of Sub-5-nm-Sized Ultrathin Nanowires

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SMALL 2020年第23期16卷 2001098-2001098页

作者： Mao, Yihua Yang, xuwen Gong, Wenbin Zhang, Jing Pan, Ting Sun, Hongzhao Chen, Zhigang Wang, Zhen Zhu, Junfa Hu, Jun Cong, Shan Geng, Fengxia zhao, Zhigang Chinese Acad Sci Key Lab Nanodevices & Applicat Suzhou Inst Nanotech & Nanobion Suzhou 215123 Peoples R China Xuzhou Univ Technol Sch Phys & Energy Xuzhou 221018 Jiangsu Peoples R China Univ Sci & Technol China Natl Synchrotron Radiat Lab Hefei 230029 Peoples R China Chinese Acad Sci Key Lab Multifunct Nanomat & Smart Syst Suzhou 215123 Peoples R China Soochow Univ Coll Energy Suzhou 215123 Peoples R China Chinese Acad Sci Suzhou Inst Nanotech & Nanobion Div Nanomat Nanchang 330200 Jiangxi Peoples R China

The high-temperature molten-salt method is an important inorganic synthetic route to a wide variety of morphological phenotypes. However, its utility is limited by the fact that it is typically incapable of producing ultrathin (<5 nm diameter) nanowires, which have a crucial role in novel nanotechnology applications. Herein, a rapid molten salt-based synthesis of sub-5-nm-sized nanowires of hexagonal tungsten oxide (h-WO3) that is critically dependent on a substantial proportion of molybdenum (Mo) dopant is described. This dopant-driven morphological transition in tungsten oxide (WO3) may be attributable to the collapse of layered structure, followed by nanocluster aggregation, coalescence, and recrystallization to form ultrathin nanowires. Interestingly, due to the structural properties of h-WO3, the thus-formed ultrathin nanowires are demonstrated to be excellent photocatalysts for the production of ammonia (NH3) from nitrogen (N-2) and water. The ultrathin nanowires exhibit a high photocatalytic NH3-production activity with a rate of 370 mu mol g(-1) h(-1) and an apparent quantum efficiency of 0.84% at 420 nm, which is more than twice that obtained from the best-performing Mo-doped W18O49 nanowire catalysts. It is envisaged that the dopant replacement-driven synthetic protocol will allow for rapid access to a series of ultrathin nanostructures with intriguing properties and increase potential applications.

关键词： dopant replacement hexagonal structures molten-salt synthesis tungsten oxide ultrathin nanostructures

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高矿化度矿井水资源化工程实践

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水处理技术 2010年第7期36卷 129-131,135页

作者：赵曙光李福勤候明刚何绪文刘双红衡水天鹰环境工程有限公司河北衡水053000 中国矿业大学化环学院北京100083 河北工程大学城建学院河北邯郸056038 汾西矿业集团公司山西介休032000

介绍了高矿化度矿井水处理工程的设计与运行参数。预处理采用中和絮凝沉淀及机械过滤工艺,其后采用反渗透除盐。实际运行结果表明,矿井水中加入25mg·L-1的PAC和0.6mg·L-1的PAM后,絮凝沉淀效果好,再经过滤及反渗透工艺处理,出... 详细信息

介绍了高矿化度矿井水处理工程的设计与运行参数。预处理采用中和絮凝沉淀及机械过滤工艺,其后采用反渗透除盐。实际运行结果表明,矿井水中加入25mg·L-1的PAC和0.6mg·L-1的PAM后,絮凝沉淀效果好,再经过滤及反渗透工艺处理,出水的电导率稳定在30μS·cm-1,可直接用于配乳用水。

关键词：矿井水高矿化度混凝沉淀机械过滤反渗透

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Identification of genetic loci associated with rough dwarf disease resistance in maize by integrating GWAS and linkage mapping

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PLANT SCIENCE 2022年 315卷 111100-111100页

作者： zhao, Meiai Liu, Shuangshuang Pei, Yuhe Jiang, xuwen Jaqueth, Jennifer S. Li, Bailin Han, Jing Jeffers, Daniel Wang, Jiabo Song, Xiyun Qingdao Agr Univ Coll Life Sci Key Lab Plant Biotechnol Univ Shandong Prov Qingdao 266109 Peoples R China Qingdao Agr Univ Coll Agron Key Lab Qingdao Major Crop Germplasm Resource Inn Qingdao 266109 Peoples R China Corteva Agrisci 7300 NW 62nd Ave Johnston IA 50131 USA Shandong Denghai Pioneer Jinan 254000 Shandong Peoples R China Yunnan Acad Agr Sci Yunnan Off Kunming 650205 Yunnan Peoples R China Southwest Minzu Univ Minist Educ Key Lab Qinghai Tibetan Plateau Anim Genet Resour Chengdu 160041 Sichuan Peoples R China

Maize rough dwarf disease (MRDD) is a viral disease that causes substantial yield loss, especially in China's summer planted maize area. Discovery of resistance genes would help in developing high-yielding resistant maize hybrids. Genome-wide association studies (GWASs) have advanced quickly and are now a powerful tool for dissecting complex genetic architectures. In this study, the disease severity index (DSI) of 292 maize inbred lines and an F6 linkage population were investigated across multiple environments for two years. Using the genotypes obtained from the Maize SNP 50K chip, a GWAS was performed with four analytical models. The results showed that 22 SNPs distributed on chromosomes 1, 3, 4, 6, 7 and 8 were significantly associated with resistance to MRDD (P<0.0001). The SNPs on chromosomes 3, 6 and 8 were consistent with the quantitative trait locus (QTL) regions from linkage mapping in an RIL population. Candidate genes identified by GWAS included an LRR receptor-like serine/threonine-protein kinase (GRMZM2G141288), and a DRE-binding protein (GRMZM2G006745). In addition, we performed an allele variation analysis of the SNP loci selected by GWAS and linkage mapping and found that the main alleles of the two SNP loci PZE_101170408 and PZE_106082685 on chromosome 1 differed in terms of disease-resistant materials and disease-susceptible materials. The identified SNPs and genes provide useful information for MRDD-related gene cloning and insights on the underlying disease resistance mechanisms, and they can be used in marker-assisted breeding to develop MRDD-resistant maize.

关键词： Maize MRDD GWAS Linkage mapping Candidate genes

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芬顿-臭氧氧化工艺用于工业园区污水处理厂技术改造

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水处理技术 2023年第7期49卷 137-141页

作者：刘娟沈军贾新强赵伟项绪文山东省环科院环境工程有限公司山东济南250013

山东某工业园污水处理厂主要处理工业废水和少量生活污水,因进水水量水质变化较大,进水和出水均超标较多。为了确保出水达标排放,新建调节池、事故池、芬顿池、臭氧接触池等对污水处理厂进行技术改造。技术改造后,CODcr、BOD5、SS、NH3-... 详细信息

山东某工业园污水处理厂主要处理工业废水和少量生活污水,因进水水量水质变化较大,进水和出水均超标较多。为了确保出水达标排放,新建调节池、事故池、芬顿池、臭氧接触池等对污水处理厂进行技术改造。技术改造后,CODcr、BOD5、SS、NH3-N、TN、TP去除率分别提升42.87%、92.71%、87.27%、52.34%、60.41%和63.63%,出水稳定达到《城镇污水处理厂污染物排放标准》(GB 18918-2002)一级A标准。

关键词：工业废水达标排放技术改造

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聚丙烯化学改性方法

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现代塑料加工应用 2010年第6期22卷 55-59页

作者：王鉴王绪文李安莲赵洪坤乔岩东北石油大学化学化工学院黑龙江大庆163318

综述了聚丙烯的化学改性方法,包括接枝、交联、共聚等,接枝主要包括熔融接枝、溶液接枝、悬浮接枝、辐射接枝、超临界CO_2协助接枝、固相接枝及非极性单体在PP上的接枝,交联改性主要包括有机氧化物交联、氮化物交联、辐射交联及等离子... 详细信息

综述了聚丙烯的化学改性方法,包括接枝、交联、共聚等,接枝主要包括熔融接枝、溶液接枝、悬浮接枝、辐射接枝、超临界CO_2协助接枝、固相接枝及非极性单体在PP上的接枝,交联改性主要包括有机氧化物交联、氮化物交联、辐射交联及等离子体交联,最后简要介绍了共聚改性以及PP改性的最新进展。

关键词：聚丙烯接枝交联共聚改性技术发展趋势

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A new nanobody-enzyme fusion protein-linked immunoassay for detecting antibodies against influenza A virus in different species

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JOURNAL OF BIOLOGICAL CHEMISTRY 2022年第12期298卷 102709页

作者： Ji, Pinpin Wang, Kun Zhang, Lu Yan, Zhenda Kong, Min Sun, xuwen Zhang, Qiang Zhou, Ning Liu, Baoyuan Zhou, En-Min Sun, Yani Wang, Xinjie zhao, Qin Northwest A&F Univ Dept Vet Prevent Med Coll Vet Med Yangling Shaanxi Peoples R China Chinese Acad Agr Sci Guangdong Lab Lingnan Modern Agr Agr Genom Inst Shenzhen Shenzhen BranchGenome Anal LabMinist Agr & Rura Shenzhen Peoples R China

Circulation of influenza A virus (IAV), especially within poultry and pigs, continues to threaten public health. A simple and universal detecting method is important for monitoring IAV infection in different species. Recently, nanobodies, which show advantages of easy gene editing and low cost of production, are a promising novel diagnostic tool for the monitoring and control of global IAVs. In the present study, five nanobodies against the nucleoprotein of H9N2 IAV were screened from the immunized Bactrian camel by phage display and modified with horseradish peroxidase (HRP) tags. Out of which, we determined that H9N2-NP-Nb5-HRP can crossreact with different subtypes of IAVs, and this reaction is also blocked by positive sera for antibodies against different IAV subtypes. Epitope mapping showed that the nanobody-HRP fusion recognized a conserved conformational epitope in all subtypes of IAVs. Subsequently, we developed a nanobody-based competitive ELISA (cELISA) for detecting anti-IAV antibodies in different species. The optimized amount of coating antigen and dilutions of the fusion and testing sera were 100 ng/well, 1:4000, and 1:10, respectively. The time for operating the cELISA was approximately 35 min. The cELISA showed high sensitivity, specificity, reproducibility, and stability. In addition, we found that the cELISA and hemagglutination inhibition test showed a consistency of 100% and 87.91% for clinical and challenged chicken sera, respectively. Furthermore, the agreement rates were 90.4% and 85.7% between the cELISA and commercial IEDXX ELISA kit. Collectively, our developed nanobody-HRP fusion–based cELISA is an ideal method for monitoring IAV infection in different species.

关键词： influenza A virus antibodies against IAVs nucleoprotein nanobody nanobody-HRP fusion protein competitive ELISA

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饥饿对不同体重组团头鲂肌肉和血清生化成分的影响

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中国农学通报 2010年第24期26卷 408-411页

作者：胡海彦宋迁红韩军涛赵永锋邴旭文中国水产科学研究院淡水渔业研究中心江苏无锡214081 河北邯郸市峰峰矿区基层动物防疫监督站河北邯郸056200

在水温20±1℃条件下,研究饥饿对不同体重团头鲂(141.3±4.23g,96.5±2.88g和76.8±3.17g)肌肉和血清生化成分的影响。结果表明各实验组体重均在饥饿14天后出现显著下降(P0.05),21～28天期间才显著下降(P0.05)。

在水温20±1℃条件下,研究饥饿对不同体重团头鲂(141.3±4.23g,96.5±2.88g和76.8±3.17g)肌肉和血清生化成分的影响。结果表明各实验组体重均在饥饿14天后出现显著下降(P<0.05),至28天时平均降幅为18.78%。饥饿过程中肌肉水分和粗灰分含量均逐步上升,粗蛋白和粗脂肪含量则持续下降,粗脂肪和粗蛋白含量分别在饥饿14天和21天时出现显著下降(P<0.05)。血清中血糖浓度相比甘油三酯和总胆固醇对饥饿更为敏感,饥饿7天后即出现显著下降(P<0.05),总胆固醇浓度在饥饿14天后差异尚不显著(P>0.05),21～28天期间才显著下降(P<0.05),甘油三酯浓度则在饥饿期间持续显著下降(P<0.05)。上述结果说明饥饿对团头鲂肌肉和血清生化成分的影响显著(P<0.05),体重差异对上述主要指标的影响不显著(P>0.05)。

关键词：团头鲂饥饿肌肉血清生化成分

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虎杖对水质和微生物群落结构及功能的影响

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环境科学与技术 2020年第5期43卷 25-32页

作者：陈家长赵志祥施羽露史磊磊郑尧邴旭文中国水产科学研究院淡水渔业研究中心农业部长江下游渔业生态环境监测中心农业部水产品质量安全环境因子风险评估实验室(无锡)江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214081

为研究虎杖种植后池塘水质变化和水体微生物群落碳源利用能力,该研究于室外构建了吉富罗非鱼养殖池塘5%虎杖生态浮床,2017年6-9月持续测定水质,利用Biolog技术分析池塘水体微生物群落结构及功能特征。结果表明6-7月生长期间虎杖种植能... 详细信息

为研究虎杖种植后池塘水质变化和水体微生物群落碳源利用能力,该研究于室外构建了吉富罗非鱼养殖池塘5%虎杖生态浮床,2017年6-9月持续测定水质,利用Biolog技术分析池塘水体微生物群落结构及功能特征。结果表明6-7月生长期间虎杖种植能有效削减池塘水体中TN、TP、Chl.a浓度,在温度较高的8、9月虎杖种植塘TP显著降低(P<0.05);7-9月虎杖种植塘水体微生物AWCD值显著增加(P<0.05),多样性McIntosh和Richness(除9月)指数均显著提高(P<0.05),且水体微生物对聚合糖类、氨基酸类、醇类的代谢能力显著提高(P<0.05)。在吉富罗非鱼养殖池塘种植虎杖,可在改善水质的基础上提高水体微生物群落多样性指数及对碳源的利用能力。

关键词：罗非鱼虎杖浮床微生物群落多样性碳源利用

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脑缺血后microPET的动态研究

脑缺血后microPET的动态研究

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中华医学会第十三次全国神经病学学术会议

作者：任传成 xuwen Gao Gang Liu Heng zhao 复旦大学附属上海市第五人民医院神经科 Department of Neurosurgery/Radiology Stanford UniversityUSA 200240

目的观察脑缺血后脑代谢的动态变化。方法雄性S-D大鼠4只,周龄11-12周,体重280-330g,dMCAO建立局灶性脑缺血模型。分别于缺血前、缺血10小时、48小时、5天行异氟烷麻醉,FDG(F-2-脱氧葡萄糖,2-[18F]fluoro-2-deoxy-D-glucose)尾静脉

关键词： microPET 缺血区梗塞面积动态研究

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