检索结果-内蒙古大学图书馆

A highly sensitive one-step nanobody-based immunoassay to specifically detect antibodies against fowl adenovirus serotype 4

引用

POULTRY SCIENCE 2025年第4期104卷 104970页

作者： Ji, Pinpin Zhang, Hao Xiri, Yangzong Li, Ziling Liu, Zhixiang Dan, Miao Li, Xiaoxuan Sun, xuwen zhao, Qin Sun, Yani Northwest A&F Univ Coll Vet Med Dept Prevent Vet Med Yangling 712100 Shaanxi Peoples R China Minist Agr Sci Observing & Expt Stn Vet Pharmacol & Diagnost Yangling 712100 Shaanxi Peoples R China

Hepatitis-hydropericardium syndrome, caused by fowl adenovirus serotype 4 (FAdV-4), has resulted in significant economic damage to the poultry industry. To monitor viral exposure and vaccine efficacy, some traditional antibody-based immunoassays have been developed for detecting anti-FAdV-4 antibodies. However, these assays have some drawbacks including multi-step operations and higher production cost. Recently, nanobodies are regarded as a promising tool for developing immunoassays. In the study, 23 nanobodies against FAdV-4 were screened and expressed with horseradish peroxidase (HRP) in the HEK293T cells. Then, the FAdV-4-Nb28-HRP fusion protein was selected for developing competitive enzyme-linked immunoassays (cELISA) to detect antiFAdV-4 antibodies in the chicken sera. The optimal concentrations and dilutions for the coating antigen, fusion protein and testing sera were determined to be 400 ng/well, 1:80 and 1:20, respectively. After the coated plates were vacuumized and stored, the operation of cELISA to detect clinical chicken sera was only one-step and the full time was 75 min. The cELISA also exhibited high sensitivity, specificity, reproducibility and good agreement with the commercial ELISA kit. When the sequential sera from the challenged chickens were tested, the cELISA showed superior sensitivity compared with the commercial ELISA kit. Moreover, epitope mapping revealed that the nanobody specifically recognized the sites GLN235 ASN236 SER238 of the fiber-1 protein, highly conserved among different FAdV-4 isolates and different from the FAdV-1 and-8. The results indicated that cELISA can specifically detect anti-FAdV-4 antibodies. Collectively, the developed one-step nanobody-based cELISA is an ideal method for epidemiological investigation and vaccine immune evaluation of FAdV-4.

关键词： Nanobody One-step competitive ELISA FAdV-4 antibodies Fiber-1 protein

来源：评论

学校读者我要写书评

暂无评论

同构型知识基础合作研发伙伴的优势

引用

科技管理研究 2018年第2期38卷 131-138页

作者：曾德明赵旭雯冯科湖南大学工商管理学院湖南长沙410082

在专利竞赛的压力下,企业普遍选择知识基础多元化、网络化路线。基于汽车产业1999—2010年专利数据和负二项混合回归模型的实证研究表明,企业自身知识基础多元化与创新产出正相关,而自身知识基础网络密度与创新产出负相关;合作研发伙伴... 详细信息

在专利竞赛的压力下,企业普遍选择知识基础多元化、网络化路线。基于汽车产业1999—2010年专利数据和负二项混合回归模型的实证研究表明,企业自身知识基础多元化与创新产出正相关,而自身知识基础网络密度与创新产出负相关;合作研发伙伴知识基础多元化正向调节自身知识基础多元化,合作研发伙伴知识基础网络密度正向调节自身知识基础网络密度。同构型合作研发伙伴有利于企业自身吸收能力的释放,对于提升其创新产出更有优势。

关键词：合作研发知识基础多元化知识基础网络创新产出

来源：评论

学校读者我要写书评

暂无评论

番茄红素通过SIRT1/NF-κB轴抑制肾癌786-O细胞增殖且促进其凋亡

引用

中国肿瘤生物治疗杂志 2023年第9期30卷 789-796页

作者：刘伟杨树芹赵序雯滕州市中心人民医院病理科山东滕州277500

目的:探讨番茄红素通过沉默信息调节因子1(SIRT1)/核因子-κB(NF-κB)轴对肾癌786-O细胞增殖、凋亡的影响。方法:常规培养人正常肾细胞HK-2和人肾癌细胞786-O,实验分为对照组(0.1%DMSO)、顺铂组(40μg/mL)、番茄红素低质量浓度(2.5μg/... 详细信息

目的:探讨番茄红素通过沉默信息调节因子1(SIRT1)/核因子-κB(NF-κB)轴对肾癌786-O细胞增殖、凋亡的影响。方法:常规培养人正常肾细胞HK-2和人肾癌细胞786-O,实验分为对照组(0.1%DMSO)、顺铂组(40μg/mL)、番茄红素低质量浓度(2.5μg/mL)组、番茄红素高质量浓度(5μg/mL)组、番茄红素(5μg/mL)+EX527(SIRT1抑制剂)(3μmol/L)组。CCK-8法、克隆形成实验检测各组HK-2、786-O细胞的增殖能力,流式细胞术检测各组786-O细胞的凋亡,RH123、DCFH-DA染色分别检测各组786-O细胞的线粒体膜电位(MMP)、活性氧(ROS)水平,WB法检测各组786-O细胞中凋亡相关蛋白BAX、Bcl-2、C-casp3和SIRT1/NF-κB轴相关蛋白SIRT1、p-NF-κB蛋白的表达。786-O细胞移植瘤实验检测番茄红素低(5 mg/kg)、高质量浓度(20 mg/Kg)、顺铂(2 mg/kg)、番茄红素(20 mg/kg)+EX527(10 mg/kg)对移植瘤生长的影响,TUNEL法检测各组移植瘤组织中的细胞凋亡。结果:番茄红素呈剂量依赖性地抑制786-O细胞的增殖活性,番茄红素、顺铂均明显抑制786-O细胞的克隆形成能力且促进其凋亡,细胞中MMP损伤率升高而ROS水平降低,凋亡相关蛋白BAX、C-casp3表达均显著升高(均P<0.05)而Bcl-2表达下调(P<0.05),SIRT1表达显著升高(P<0.05)而p-NF-κB的表达显著降低(P<0.05),上述作用均可被EX527逆转;番茄红素、顺铂抑制786-O细胞移植瘤的生长且促进其细胞凋亡,其作用也能被EX527逆转。结论:番茄红素通过上调SIRT1、抑制NF-κB通路的激活进而抑制786-O细胞增殖且诱导其凋亡。

关键词：肾癌 786-O细胞番茄红素沉默信息调节因子1 核因子-κB 增殖凋亡

来源：评论

学校读者我要写书评

暂无评论

Combining UAV multisensor field phenotyping and genome-wide association studies to reveal the genetic basis of plant height in cotton (Gossypium hirsutum)

引用

PLANT PHENOMICS 2025年第1期7卷

作者： Fan, Liqiang Yang, Jiajie Wang, xuwen Liu, zhao Xu, Bowei Liu, Li Gao, Chenxu Ai, Xiantao Li, Fuguang Gao, Lei Yu, Yu Yang, Zuoren Chinese Acad Agr Sci Inst Cotton Res State Key Lab Cotton Biobreeding & Integrated Util Anyang 455000 Henan Peoples R China CAAS Inst Western Agr Xinjiang Key Lab Crop Gene Editing & Germplasm Inn Changji 831100 Xinjiang Peoples R China Xinjiang Acad Agr & Reclamat Sci Cotton Res Inst Northwest Inland Reg Key Lab Cotton Biol & Genet B Minist Agr Urumqi Peoples R China Zhengzhou Univ Sch Agr Sci State Key Lab Cotton Biobreeding & Integrated Util Zhengzhou Res Base Zhengzhou 450001 Henan Peoples R China Xinjiang Univ Res Inst Coll Smart Agr Urumqi 830046 Xinjiang Peoples R China Xinjiang Agr Univ Coll Agr Engn Res Ctr Cotton Minist Educ 311 Nongda East Rd Urumqi 830052 Peoples R China

Plant height (PH) is a key agronomic trait influencing plant architecture. Suitable PH values for cotton are important for lodging resistance, high planting density, and mechanized harvesting, making it crucial to elucidate the mechanisms of the genetic regulation of PH. However, traditional field PH phenotyping largely relies on manual measurements, limiting its large-scale application. In this study, a high-throughput phenotyping platform based on UAV-mounted RGB and light detection and ranging (LiDAR) was developed to efficiently and accurately obtain time series PHs of 419 cotton accessions in the field. Different strategies were used to extract PH values from two sets of sensor data, and the extracted values were used to train using linear regression and machine learning methods to obtain PH predictions. These predictions were consistent with manual measurements of the PH for the LiDAR (R2 1/4 0.934) and RGB (R2 1/4 0.914) data. The predicted PH values were used for GWAS analysis, and 34 PH-related genes, two of which have been demonstrated to regulate PH in cotton, namely, GhPH1 and GhUBP15, were identified. We further identified significant differences in the expression of a new gene named GhPH_UAV1 in the stems of the G. hirsutum cultivar ZM24 harvested on the 15th, 35th, and 70th days after sowing compared with those from a dwarf mutant (pag1), which presented shortened stem and internode phenotypes. The overexpression of GhPH_UAV1 significantly promoted cotton stem development, whereas its knockout by CRISPR-Cas9 dramatically inhibited stem growth, suggesting that GhPH_UAV1 plays a positive regulatory role in cotton PH. This field-scale high-throughput phenotype monitoring platform significantly improves the ability to obtain high-quality phenotypic data from large populations, which helps overcome the imbalance between massive genotypic data and the shortage of field phenotypic data and facilitates the integration of genotype and phenotype research for cr

关键词： Plant height UAV RGB LiDAR Genome-wide association study

来源：评论

学校读者我要写书评

暂无评论

Harnessing conversion bridge strategy by organic semiconductor in polymer matrix memristors for high-performance multi-modal neuromorphic signal processing

引用

INFOMAT 2025年第5期7卷

作者： Dong, Weijia Ji, Xuan An, Chuanbin Xu, Chenhui Zhang, xuwen zhao, Bin Liu, Yuqian Wang, Shiyu Yu, Xi Liu, Xinjun Han, Yang Geng, Yanhou Tianjin Univ Sch Mat Sci & Engn Tianjin Key Lab Mol Optoelect Sci Minist EducCollaborat Innovat Ctr Chem Sci & Engn Tianjin 300350 Peoples R China Tianjin Univ Collaborat Innovat Ctr Chem Sci & Engn Tianjin Key Lab Organ Integrated Circuits Minist Educ Tianjin Peoples R China Tianjin Univ Sch Sci Dept Chem Key Lab Organ Integrated CircuitMinist Educ Tianjin 300072 Peoples R China Tianjin Univ Sch Sci Dept Chem Tianjin Key Lab Mol Optoelect Sci Tianjin 300072 Peoples R China Tianjin Univ Joint Sch Natl Univ Singapore & Tianjin Univ Int Campus Fuzhou Peoples R China Trina Solar State Key Lab Photovolta Sci & Technol Changzhou Peoples R China Tianjin Univ Fac Sci Tianjin Key Lab Low Dimens Mat Phys & Preparat Tec Tianjin 300354 Peoples R China

Organic memristors, integrating chemically designed resistive switching and mechanical flexibility, present promising hardware opportunities for neuromorphic computing, particularly in the development of next-generation wearable artificial intelligence devices. However, challenges persist in achieving high yield, controllable switching, and multi-modal information processing. In this study, we introduce an efficient distribution of conversion bridges (EDCB) strategy by dispersing organic semiconductor (poly[2,5-bis(3-tetradecylthiophen-2-yl)thieno[3,2-b]thiophene], PBTTT) in elastomer (polystyrene-block-poly(ethylene-ran-butylene)-block-polystyrene, SEBS). This innovative approach results in memristors with exceptional yield, high stretchability, and reliable switching performance. By fine-tuning the semiconductor content, we shift the primary charge carriers from ions to electrons, realizing modulable non-volatile, and volatile duo-mode memristors. This advancement enables multi-modal signal processing at distinct operational mechanisms-non-volatile mode for image recognition in convolutional neural networks (CNNs) and volatile mode for dynamic classification and prediction in reservoir computing (RC). A fully analog RC hardware system is further demonstrated by integrating the distinct volatile and non-volatile modes of the EDCB-based memristor into the dynamic neuron network and the linear regression layer of the RC respectively, achieving high accuracy in online arrhythmia detection tasks. Our work paves the way for high-yield organic memristors with mechanical flexibility, advancing efficient multi-mode neuromorphic computing within a unified memristor system integrating volatile and non-volatile ***

关键词： convolutional neural network neuromorphic computing organic memristors reservoir computing stretchable electronics

来源：评论

学校读者我要写书评

暂无评论

A synergistic water-saving strategy combining stage-specific irrigation and application of exogenous thiamine enhances drought resistance of Xinjiang cotton

引用

PLANT AND SOIL 2025年 1-14页

作者： zhao, Fuxiang Xia, Linjie Sun, Simin Li, Hang Rong, Yuxuan Wang, Yuxin Wang, xuwen Kong, Xianhui Zhu, Longfu Zhang, Xianlong Yu, Yu Yang, Xiyan Xinjiang Acad Agr & Reclamat Sci Cotton Inst Shihezi 832000 Xinjiang Peoples R China Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan 430070 Hubei Peoples R China Hubei Hongshan Lab Wuhan Peoples R China

Background and aimsXinjiang is the largest cotton planting area in China. However, the perennial water shortage in Xinjiang restricts the development of the cotton industry, making drought one of the important limiting factors. The aim of this study was to evaluate the effectiveness of water-saving scheme and thiamine (Thi) application on alleviating the adverse effects of drought stress on the growth and development of *** this study, five Xinjiang-released cotton varieties (Jinken1775, Jinken1565, Huamian702, Zhongmian979, and Jinfeng2) were selected as the research object, and Xinluzao8 served as the drought-sensitive control. Comprehensive drought resistance index (D) of the drought resistance coefficient (DRC) of four yield and four quality traits were used to evaluate the drought resistant ability of the six cotton varieties under different water conditions and Thi spraying *** stage-specific irrigation strategy (60% during vegetative vs. 90% during the flowering and boll stage) concurrently maintained higher drought resistance across cultivars, with Jinken1565 exhibited the highest drought resistance. This scheme reduced irrigation by 26% while sustaining fiber quality. Exogenous 50 mM Thi further enhanced drought tolerance, reducing transpiration rate by 33% and blade damage by 55%. These results demonstrate that stage-specific water management optimizes drought adaptation in cotton cultivation under drought *** study suggests that a 26% water-saving scheme was proposed for most drought-resistant varieties planted in Xinjiang, and an appropriate scheme to achieve stable yield by Thi application during the early drought condition was proposed for varieties with low drought resistant ability.

关键词： Cotton Drought resistance Comprehensive drought resistance index Thiamine Water saving scheme

来源：评论

学校读者我要写书评

暂无评论

Finite element analysis of corn kernel damage: a viscoelastic model approach for impact-induced damage prediction

引用

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 2025年第7期105卷 3993-4004页

作者： zhao, Xuelin Zhang, Qiang Fang, xuwen Zhang, Jinsong Feng, Weijing Zhang, Li Zhou, Deyi Yu, Chunsheng Wang, Hao Gao, Xiaodi Zhou, Haigen Wu, Xinling Jilin Univ Coll Biol & Agr Engn Changchun 130022 Peoples R China

BACKGROUNDIn the present study, we address the quality degradation of corn during post-harvest processing by developing a viscoelastic composite model to predict kernel damage during post-harvest processing. The model, based on high-resolution scanning and inverse modeling techniques, provides an accurate representation of the complex internal components of corn kernels, which allows for the analysis of their stress response and damage susceptibility trends under different impact *** results show that the viscoelastic model has a relative error of 2.4% compared to the drop test data, thus demonstrating that the viscoelastic model has a very high accuracy in predicting impact damage and is able to accurately localize the flour-like endosperm as the main region of impact damage. The model showed a high correlation (0.99) between predicted and experimental damage rates by response surface *** damage during processing is reduced by reducing the proportion of flour-like endosperm in maize kernels. A new method for reducing damage during food handling and processing operations is proposed, providing an important reference for improving kernel durability and contributing to the development of gentler processing techniques to improve the quality of maize products. (c) 2025 Society of Chemical Industry.

关键词： viscoelastic composite model collision damage finite element method burgers model high moisture corn kernel

来源：评论

学校读者我要写书评

暂无评论

长段型先天性巨结肠的临床诊治进展

引用

医学综述 2022年第16期28卷 3259-3263页

作者：赵绪稳胡博天津市儿童医院新生儿外科天津300074

先天性巨结肠又称希尔施普龙病(HD),是儿童常见的消化道发育畸形疾病,有家族遗传倾向,常影响患儿生长发育,也是造成新生儿及小婴儿急性肠梗阻的常见原因之一。尤其是长段型HD,由于狭窄段肠管较长,生后多有胎便排出延迟病史,并且早期即... 详细信息

先天性巨结肠又称希尔施普龙病(HD),是儿童常见的消化道发育畸形疾病,有家族遗传倾向,常影响患儿生长发育,也是造成新生儿及小婴儿急性肠梗阻的常见原因之一。尤其是长段型HD,由于狭窄段肠管较长,生后多有胎便排出延迟病史,并且早期即可出现排便困难、腹胀甚至呕吐等症状,伴随病情进展,并对消化系统造成渐进性损害,可诱发巨结肠相关小肠结肠炎,也是导致患儿死亡的重要原因。手术是唯一有效的根治方法,虽然腹腔镜下根治术明显减少了开腹手术的并发症,手术安全性有所提高并取得巨大进步,但以常见型巨结肠的报道居多,对于手术方式仍有争议。

关键词：长段型先天性巨结肠肠梗阻腹腔镜

来源：评论

学校读者我要写书评

暂无评论

高频超声对新生儿坏死性小肠结肠炎需手术治疗的预测价值

引用

中华新生儿科杂志（中英文） 2024年第1期39卷 18-22页

作者：杨薇裴广华徐魏军赵绪稳天津市儿童医院(天津大学儿童医院)超声科天津300074 天津市儿童医院(天津大学儿童医院)新生儿外科天津300074

目的探讨高频超声对新生儿坏死性小肠结肠炎(necrotizing enterocolitis,NEC)需手术治疗的预测价值。方法选择2018年1月至2021年12月天津市儿童医院新生儿外科诊断为Bell分期Ⅱ期以上的NEC患儿进行回顾性分析。根据是否手术分为手术组... 详细信息

目的探讨高频超声对新生儿坏死性小肠结肠炎(necrotizing enterocolitis,NEC)需手术治疗的预测价值。方法选择2018年1月至2021年12月天津市儿童医院新生儿外科诊断为Bell分期Ⅱ期以上的NEC患儿进行回顾性分析。根据是否手术分为手术组和非手术组,比较两组超声检查肠蠕动、肠壁血流灌注、肠壁积气、门静脉积气、腹腔积液内透声、腹腔积液深度、肠壁厚度情况,采用logistic回归分析NEC患儿需手术干预的危险因素,并做受试者工作特征曲线分析,计算联合因子和单一因子预测NEC患儿急性期需手术治疗的价值。结果共纳入40例,手术组18例,非手术组22例。两组超声检查肠壁积气、腹腔积液深度差异无统计学意义(P>0.05);手术组肠蠕动减弱、门静脉积气、肠壁血流灌注减少、腹腔积液透声差比例均高于非手术组,肠壁厚度小于非手术组(P<0.05)。Logistic回归分析显示,腹腔积液透声差、肠壁变薄、肠壁血流灌注减少及肠蠕动减弱对NEC患儿需手术治疗有较高的预测效能。受试者工作特征曲线分析显示预测NEC患儿需手术治疗的肠壁厚度临界值为1.2 mm,曲线下面积0.746,敏感度87.5%,特异度65.6%;联合因子预测NEC患儿需手术干预的曲线下面积为0.867,敏感度96.9%,特异度75.8%。结论高频超声显示肠蠕动减弱、肠壁血流灌注减少、肠壁变薄及腹腔积液内透声差对NEC急性期是否需手术治疗有较高的预测效能。

关键词：新生儿坏死性小肠结肠炎超声肠壁积气门静脉积气

来源：评论

学校读者我要写书评

暂无评论

A Methionine Allocation Nanoregulator for the Suppression of Cancer Stem Cells and Support to the Immune Cells by Epigenetic Regulation

引用

ADVANCED SCIENCE 2025年第15期12卷 e2415207页

作者： Su, Boyu Chen, Qinjun Li, xuwen Fang, Mingzhu Wang, Yu Song, Haolin You, Haoyu Zhou, Zheng Wu, Yuxing zhao, Zhenhao Chen, Yun Fan, Hongrui Li, Chufeng Jiang, Chen Sun, Tao Fudan Univ State Key Lab Med Neurobiol Sch Pharm Dept PharmaceutKey Lab Smart Drug DeliveryMinist Shanghai 201203 Peoples R China MOE Frontiers Ctr Brain Sci Shanghai 201203 Peoples R China Fujian Med Univ Affiliated Hosp 1 Natl Reg Med Ctr Dept Digest Dis Binhai Campus Fuzhou 350212 Peoples R China Quzhou Fudan Inst Quzhou 324003 Peoples R China

Epigenetic dysregulation is prevalent in human cancers, affecting gene expression and metabolic patterns to meet the demands of malignant evolution and abnormal epigenetic processes, and resulting in a protumor immune microenvironment. Tumors require a steady supply of methionine for maintaining epigenetic flexibility, which is the only exogenous precursor of methyl donor S-adenosylmethionine for methylation, crucial for their resistance to therapies and survival in a nutrient-deficient microenvironment. Thus, tumor cells upregulate the Lat4 transporter to compete and deprive methionine in the microenvironment, sustaining their malignant phenotypes and also impairing immune cell functions. Addressing this methionine addiction is the key to overcoming drug resistance and improving immune response. Despite the challenge of lacking specific Lat4 inhibitors, an oxaliplatin prodrug crosslinked fluorinated polycation/anti-Lat4 small interfering RNA complex nanoregulator (AS-F-NP) has been designed and developed here. This nanoregulator restricted the greedy methionine uptake of tumor cells by knocking down Lat4, which in turn inhibited the malignant evolution of the tumor while restoring the viability and function of tumor-infiltrating immune cells.

关键词： cancer therapy epigenetic immune methionine oxaliplatin siRNA

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：