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Synthesis and bioactivity of thiazolidin-2-cyanamide derivatives against type III secretion system of Xanthomonas oryzae on rice

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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 2018年第Jul.期149卷 89-97页

作者： Xiang, xuwen Tao, Hui jiang, Shan Zhang, Lian-Hui Cui, Zi-Ning South China Agr Univ State Key Lab Conservat & Utilizat Subtrop Agrobi Integrat Microbiol Res Ctr Guangdong Prov Key Lab Microbial Signals & Dis Co Guangzhou 510642 Guangdong Peoples R China Guizhou Univ Minist Educ Key Lab Green Pesticide & Agr Bioengn Guiyang 550025 Guizhou Peoples R China

Targeting virulence factors of bacterial without affecting their growth and survival, has been an initiative strategy for the development of novel anti-microbial agents. The type III secretion system (T3SS), one of essential and highly conserved virulence factors in most Gram-negative pathogenic bacteria, has been regarded as an effective target that developed new anti-microbial drugs. Xanthomonas oryzae pv. oryzae (Xoo) is one of the most Important bacterial pathogens on rice, which causes leaf blight disease. To discover potential anti-virulence agents against the pathogens, a new series of thiazolidin-2-cyanamide derivatives containing 5-phenyl-2-furan were designed and synthesized. Their structures were characterized by H-1 NMR, C-13 NMR, MS, and elemental analysis. All the title compounds inhibited the promoter activity of a harpin gene hpa1, significantly, that were further checked for the impact on bacterial growth and on the hypersensitive response (HR) caused by Xoo on non-host tobacco plants. The results indicated that treatment of Xoo with the title compounds II-2,II-3 and II-4 resulted in significantly attenuated HR without affecting bacterial growth or survival. Moreover, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that the expression of the Xoo T3SS was suppressed by treatment with the three inhibitors. The mRNA levels of representative genes in the hrp (hypersensitive response and pathogenicity) cluster, as well as the regulatory genes hrpG and hrpX, were reduced. Finally, the in vivo test demonstrated that the compounds could reduce the disease symptoms of Xoo on the rice cultivar (Oryza sativa) IR24.

关键词： Xanthomonas oryzae pv. oryzae (Xoo) Type III secretion system (T3SS) Small molecule inhibitors Virulence factors Thiazolidin-2-cyanamide Synthesis

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Epidemiological investigation of feline infectious peritonitis in cats living in Harbin, Northeast China from 2017 to 2019 using a combination of an EvaGreen-based real-time RT-PCR and serum chemistry assays

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MOLECULAR AND CELLULAR PROBES 2020年第0期49卷 101495-000页

作者： Guan, Xueting Li, Hua Han, Meijing Jia, Shuo Feng, Baohua Gao, xuwen Wang, Zhuo jiang, Yanping Cui, Wen Wang, Li Xu, Yigang Northeast Agr Univ Heilongjiang Key Lab Anim Dis Control & Pharmaceu Harbin Peoples R China Minist Agr China Key Lab Anim Pathogen Biol Northeast Sci Inspect Stn Harbin Peoples R China

Feline infectious peritonitis (FIP) is caused by the FIP virus (FIPV), a highly virulent mutant form of feline coronavirus (FCoV). This disease is one of the most important infectious diseases in cats, and it is associated with high mortality, particularly among younger cats. In this study, we isolated a wild-type FIPV HRB-17 epidemic strain from the blood sample of household pet cat exhibiting the characteristic wet-form FIP symptoms, which has been confirmed further by animal infection. Further, we developed an EvaGreen-based real-time RT-PCR assay for the accurate detection of FCoV based on the amplification of the highly conserved FIPV N gene. Then, using a combination of the real-time RT-PCR approach and a serum chemistry assay, we performed an epidemiological survey of FIPV infection in cats living in Harbin City, Northeast China. The results indicated that the EvaGreen-based real-time RT-PCR assay can be used for screening FCoV infection in the affected cats at an analytical detection limit of 8.2 x 10(1) viral genome copies/A, but could not effectively distinguish FIPVs from FECVs. Additionally, the results of the epidemiological survey investigating feline blood samples (n = 1523) collected between July 2017 to July 2019 revealed an FIPV prevalence of approximately 12% (189/1523). Maybe, the prevalence would be less than 12% due to the real-time RT-PCR assay could not accurately differentiate FIPV and FECV. Nevertheless, it still highlighted the severity of the FIP epidemic in cats and reiterated the urgent need to develop effective anti-FIP therapeutic agents and anti-FIPV vaccines. As pet cats are household animals, risk communication and continuous region-extended surveillance cat programs are recommended.

关键词： Feline infectious peritonitis virus N gene EvaGreen-based real-time 12T-PCR Hyperglobulinemia Epidemiological survey

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Research on Virtual Power Plant's Participation in Power Market under the Background of Energy Internet in China

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IOP Conference Series: Earth and Environmental Science 2021年第1期621卷

作者： Yang Su Chen Kening Wang Yang jiang Yu Ding Yu Liu xuwen Ma Qiuyang State Grid Energy Research Institute Beijing 102209 China State Grid JiangSu Electric Power Co. Ltd. JiangSu 210008 China

Under the background of energy internet, a large influx of market entities participates in the interaction of 'source network and load storage' and becomes an important resource for system regulation through the electricity marketization. Among them, the virtual power plant involves several market participators such as the distributed generation, demand side adjustable resources, distributed energy storage. The research of the operation mechanism and market-oriented trading organization for the virtual power plant becomes a popular topic in recent years. This paper proposes a novel mechanism for virtual power plants to participate in the electricity trading market, including the trading categories and auxiliary service which can be provided by the virtual power plant.

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Macrophage-Disguised Manganese Dioxide Nanoparticles for Neuroprotection by Reducing Oxidative Stress and Modulating Inflammatory Microenvironment in Acute Ischemic Stroke

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ADVANCED SCIENCE 2021年第20期8卷 e2101526页

作者： Li, Chao Zhao, Zhenhao Luo, Yifan Ning, Tingting Liu, Peixin Chen, Qinjun Chu, Yongchao Guo, Qin Zhang, Yiwen Zhou, Wenxi Chen, Hongyi Zhou, Zheng Wang, Yu Su, Boyu You, Haoyu Zhang, Tongyu Li, xuwen Song, Haolin Li, Chufeng Sun, Tao jiang, Chen Fudan Univ Sch Pharm Dept PharmaceutState Key Lab Med Neurobiol Key Lab Smart Drug DeliveryMinist EducInst Brai Shanghai 201203 Peoples R China Fudan Univ Sch Pharm Inst Brain Sci MOE Frontiers Ctr Brain SciDept Pharmaceut Shanghai 201203 Peoples R China

Reperfusion injury is still a major challenge that impedes neuronal survival in ischemic stroke. However, the current clinical treatments are remained on single pathological process, which are due to lack of comprehensive neuroprotective effects. Herein, a macrophage-disguised honeycomb manganese dioxide (MnO2) nanosphere loaded with fingolimod (FTY) is developed to salvage the ischemic penumbra. In particular, the biomimetic nanoparticles can accumulate actively in the damaged brain via macrophage-membrane protein-mediated recognition with cell adhesion molecules that are overexpressed on the damaged vascular endothelium. MnO2 nanosphere can consume excess hydrogen peroxide (H2O2) and convert it into desiderated oxygen (O-2), and can be decomposed in acidic lysosome for cargo release, so as to reduce oxidative stress and promote the transition of M1 microglia to M2 type, eventually reversing the proinflammatory microenvironment and reinforcing the survival of damaged neuron. This biomimetic nanomedicine raises new strategy for multitargeted combined treatment of ischemic stroke.

关键词： biomimetic nanoparticles inflammatory microenvironment microglia polarization neuroprotection O-2 generation reperfusion injury reactive oxygen species (ROS) consumption

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StraPep: a structure database of bioactive peptides

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DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION 2018年第2018期2018卷 1-N.PAG页

作者： Wang, Jian Yin, Tailang Xiao, xuwen He, Dan Xue, Zhidong jiang, Xinnong Wang, Yan Huazhong Univ Sci & Technol Coll Life Sci & Technol Key Lab Mol Biophys Minist Educ Wuhan 430074 Hubei Peoples R China Wuhan Univ Renmin Hosp Reprod Med Ctr Wuhan 430060 Hubei Peoples R China Huazhong Univ Sci & Technol Sch Software Engn Wuhan 430074 Hubei Peoples R China Sun Yat Sen Univ Affiliated Hosp 1 Dept Neurol Guangzhou 510080 Guangdong Peoples R China

Bioactive peptides, with a variety of biological activities and wide distribution in nature, have attracted great research interest in biological and medical fields, especially in pharmaceutical industry. The structural information of bioactive peptide is important for the development of peptide-based drugs. Many databases have been developed cataloguing bioactive peptides. However, to our knowledge, database dedicated to collect all the bioactive peptides with known structure is not available yet. Thus, we developed StraPep, a structure database of bioactive peptides. StraPep holds 3791 bioactive peptide structures, which belong to 1312 unique bioactive peptide sequences. About 905 out of 1312 (68%) bioactive peptides in StraPep contain disulfide bonds, which is significantly higher than that (21%) of PDB. Interestingly, 150 out of 616 (24%) bioactive peptides with three or more disulfide bonds form a structural motif known as cystine knot, which confers considerable structural stability on proteins and is an attractive scaffold for drug design. Detailed information of each peptide, including the experimental structure, the location of disulfide bonds, secondary structure, classification, post-translational modification and so on, has been provided. A wide range of user-friendly tools, such as browsing, sequence and structure-based searching and so on, has been incorporated into StraPep. We hope that this database will be helpful for the research community.

关键词： MOLECULAR structure of peptides BIOLOGICAL databases PROTEIN stability

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抗坏血酸对铜胁迫下广藿香幼苗生长、铜积累和抗氧化酶活性的影响

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环境化学 2016年第7期35卷 1431-1437页

作者：李贺勤王维华江绪文青岛农业大学农学与植物保护学院/山东省旱作农业技术重点实验室青岛266109

通过室内水培试验,研究了外源添加抗坏血酸(Ascorbic acid,As A)对铜(Copper,Cu)胁迫下广藿香(Pogostemon cablin(Blanco)Benth.)幼苗生长、Cu^(2+)积累和抗氧化酶活性的影响.结果显示,与对照相比,10—80μmol·L-1Cu^(2+)降低了广... 详细信息

通过室内水培试验,研究了外源添加抗坏血酸(Ascorbic acid,As A)对铜(Copper,Cu)胁迫下广藿香(Pogostemon cablin(Blanco)Benth.)幼苗生长、Cu^(2+)积累和抗氧化酶活性的影响.结果显示,与对照相比,10—80μmol·L-1Cu^(2+)降低了广藿香幼苗的苗长、根长、植株生物量和根系耐性指数,且随Cu^(2+)浓度增大抑制作用增强.与Cu^(2+)胁迫相比,外源添加0.10—0.50 mmol·L^(-1)As A提高了广藿香幼苗的苗长、根长、植株生物量、根系耐性指数、根系活力以及SOD、POD、CAT和APX酶活性,降低其根系相对电导率、MDA含量和Cu^(2+)含量;随As A浓度的增加,苗长、根长、植株生物量、根系耐性指数、根系活力以及POD和CAT活性先升高后下降,相对电导率、MDA含量和Cu^(2+)含量先下降后上升,SOD和APX活性则一直增加.结果显示,外源添加0.25 mmol·L^(-1)的As A能提高广藿香幼苗细胞清除活性氧的能力,一定程度上缓解40μmol·L^(-1)Cu^(2+)胁迫对膜系统的伤害,减轻Cu^(2+)胁迫对广藿香幼苗生长的毒害作用.

关键词：广藿香铜胁迫抗坏血酸幼苗生长抗氧化酶

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Determination of common ginsenosides in Kang'ai injection by aqueous two-phase extraction with deep eutectic solvents and HPLC-UV/DAD

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MICROCHEMICAL JOURNAL 2018年 137卷 302-308页

作者： Li, Peng Zhao, Pengyun Liu, Weijie jiang, Yifu Wang, Wenjiao Bao, Longyue Jin, Yongri Li, xuwen Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China

In this work, four kinds of deep eutectic solvents (DESs), composed of choline chloride (ChCl) and several alcohols, were synthesized and characterized by H-1 NMR and FT-IR spectra. Based on DES ( ChCl and 1,4-butanediol) and inorganic salt solution (dipotassium phosphate, K2HPO4), aqueous two-phase system (ATPS) coupled with high-performance liquid chromatography has been developed and applied. This method was used for the efficient extraction and determination of eight common ginsenosides (including ginsenoside Rg(1), Re, Rf, Rg(2), Rc, Rb-2 and Rd) in the traditional Chinese medicine injection named Kang'ai injection. To achieve optimum extraction performance, the experimental conditions were optimized, including type and amount of DES and salt, ultrasound time, temperature and pH value. In the dynamic linearity range, experimental results showed satisfactory recoveries of analytes ranging from 92.7-110.8% with the relative standard deviations (RSDs) ranging from 1.2-3.9%. It has been proved that DES-ATPS has great potential for determination of active ingredients in traditional Chinese medicine injection as well as samples in other analytical science field. (C) 2017 Elsevier B.V. All rights reserved.

关键词： Aqueous two-phase system Ginsenosides High-performance liquid chromatography Kang'ai injection

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吉富罗非鱼养殖池塘集成调控技术配比研究

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中国农学通报 2021年第33期37卷 148-152页

作者：孙旋辉蒋咏燚刘文婷郑尧陈家长邴旭文中国水产科学研究院淡水渔业研究中心南京农业大学无锡渔业学院江苏无锡214081

前期研究表明"5%空心菜+10%填料+泼藻"水质调控集成模式值得推广,为进一步验证效果,以吉富罗非鱼养殖池塘为对象,研究不同空心菜种植配比其对池塘水质氮、磷净化效果。通过设置5%、8%和10%空心菜种植比例,测定TN、NH;-N、TP、... 详细信息

前期研究表明"5%空心菜+10%填料+泼藻"水质调控集成模式值得推广,为进一步验证效果,以吉富罗非鱼养殖池塘为对象,研究不同空心菜种植配比其对池塘水质氮、磷净化效果。通过设置5%、8%和10%空心菜种植比例,测定TN、NH;-N、TP、CODMn、TSS、p H和DO等水质指标,并进行环境、效益评估。结果表明:空心菜种植对罗非鱼池塘水体中TN、NH;-N、TP、CODMn、TSS均有一定程度的降低,空心菜种植对TN去除率在10%~15%之间,对TP去除率在20%~40%之间,5%空心菜种植组还对NH;-N、CODMn有较好的去除效果,且效益较好适合推广。

关键词：池塘水质调控原位修复集成中草药鱼菜配比营养盐去除效果成本核算

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Determination of IgG by electron spin resonance spectroscopy using Fe₃O₄ nanoparticles as probe

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MICROCHEMICAL JOURNAL 2018年 141卷 444-450页

作者： Tian, Sizhu jiang, Jia Zang, Shuang Wang, Kun Yu, Yong Li, xuwen Zhang, Hanqi Yu, Aimin Zhang, Ziwei Jilin Univ Coll Chem Qianjin St 2699 Changchun 130012 Jilin Peoples R China Jilin Univ Coll Instrumentat & Elect Engn West Minzhu St 938 Changchun 130061 Jilin Peoples R China

A novel electron spin resonance (ESR) spectroscopy method for determination of rabbit IgG was presented. Anti rabbit IgG antibody was bound to Fe3O4 nanoparticles (FeNPs). The FeNPs were used as probe to give ESR signal. After the optimization of the assay, a molar ratio 500:1 of antibody to FeNPs was selected. The sepharose beads were used as solid phase and used to separate the analyte from the sample solution. Two typical immunoassay formats, sandwich and competitive formats, were applied to the determination of IgG. The analytical sensitivities obtained by sandwich and competitive formats were 0.13 mu g mL(-1) and 0.56 mu g mL(-1), respectively. The two formats were applied to the determination of the rabbit IgG in rabbit serum. The diluted sample was directly analyzed and other additional sample treatment was not required. The analytical results of the spiked samples indicated that the recoveries of the analytes were acceptable. Furthermore, the sample preparation time used in this method is shorter than most existing immunoassay methods.

关键词： Electron spin resonance Sandwich Competitive Fe3O4 nanoparticles Sepharose beads Rabbit serum

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Histopathological analysis and the immune related gene expression profiles of mandarin fish (Siniperca chuatsi) infected with Aeromonas hydrophila

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FISH & SHELLFISH IMMUNOLOGY 2018年 83卷 410-415页

作者： Chen, Nan jiang, Jingjing Gao, Xiaojian Li, Xixi Zhang, Yue Liu, Xiaodan Yang, Hui Bing, xuwen Zhang, Xiaojun Yangzhou Univ Coll Anim Sci & Technol Yangzhou 225009 Jiangsu Peoples R China Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Key Lab Freshwater Fisheries & Germplasm Utilizat Minist Agr Wuxi 214081 Peoples R China

Hemorrhagic septicemia of mandarin fish (Siniperca chuatsi) was mainly caused by Aeromonas hydrophila which was an opportunistic pathogen. In recent years, the disease has caused tremendous economic loss with high morbidity and mass mortality in the mandarin fish breeding industry. Histopathological analysis and the immune related gene expression profiles of mandarin fish (S. chuatsi) infected with A. hydrophila were investigated in this study. Transmission electron microscopy (TEM) images showed that the cells of A. hydrophila densely covered with a mass of fimbriae. Histopathological analysis revealed that inflammation, vacuolization and extensive necrosis existed in the gill, liver, spleen and head kidney of the diseased fish. Quantitative real-time PCR was performed to measure mRNA expression levels for six immune related genes in mandarin fish after A. hydrophila infection. The transcriptional analysis of these immune related genes demonstrated that the expression levels of major histocompatibility complex class II (MHC II), T cell receptor alpha (TCR alpha), tumor necrosis factor alpha (TNF alpha), CC chemokine 3, interleukin 8 (IL-8) and Hepcidin were strongly up-regulated in spleen and head kidney of mandarin fish post-infection. These results will contribute to further study on the pathogenesis and host defensive system in A. hydrophila infection.

关键词： Mandarin fish Aeromonas hydrophila Histopathology Immune related genes

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