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Low-Triggering-Potential and Narrow-Potential-Window Electrochemiluminescence of Silver Nanoclusters for Gene Assay

ANALYTICAL CHEMISTRY 2024年第17期96卷 6652-6658页

作者： Ai, Yaojia Gao, xuwen Ren, Xiaoxuan li, Mengwei Zhang, Bin Zou, Guizheng Shandong Univ Sch Chem & Chem Engn Jinan 250100 Peoples R China

A low-triggering potential and a narrow-potential window are anticipated to decrease the electrochemical interference and cross talk of electrochemiluminescence (ECL). Herein, by exploiting the low oxidative potential (0.82 V vs Ag/AgCl) of dihydrolipoic acid-capped sliver nanoclusters (DHLA-AgNCs), a coreactant ECL system of DHLA-AgNCs/hydrazine (N2H4) is proposed to achieve efficient and oxidative-reduction ECL with a low-triggering potential of 0.82 V (vs Ag/AgCl) and a narrow-potential window of 0.22 V. The low-triggering-potential and narrow-potential-window nature of ECL can be primarily preserved upon labeling DHLA-AgNCs to probe DNA and immobilizing DHLA-AgNCs onto the Au surface via sandwiched hybridization, which eventually enables a selective ECL strategy for the gene assay at +0.82 V. This gene assay strategy can sensitively determine the gene of human papillomavirus from 10 to 1000 pM with a low limit of detection of 5 pM (S/N = 3) and would open a way to improve the applied ECL bioassay.

关键词： Assays Electrodes Gold Particulate matter Precursors

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Binding characteristics of Pb and Zn to low-temperature feces-based biochar-derived DOM revealed by EEM-PARAFAC combined with general and moving-window two-dimensional correlation analyses

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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 2023年第10期30卷 27525-27538页

作者： He, Changjun He, xuwen Yuan, Run li, Na Jiang, Jinyuan Chinese Res Inst Environm Sci Res Ctr Environm Pollut Control Technol Beijing 100012 Peoples R China China Univ Min & Technol Beijing Sch Chem & Environm Engn Beijing 100083 Peoples R China

Pyrolysis carbonization of human feces has shown potential for converting feces biomass into a soil amendment. However, little is known about the interactions of DOM derived from feces-based biochar produced at low-temperature with heavy metals (HMs). In this study, the binding properties of Pb(II) and Zn(II) with DOM derived from feces-based biochar produced at low pyrolysis temperatures were investigated using EEM-PARAFAC combined with general, and moving-window two-dimensional correlation analyses (2D-COS). The results revealed that DOM from biochar produced at 280 degrees C exhibited a higher Pb(II) and Zn(II) affinity and more binding sites than DOM produced at 380 degrees C. The fulvic-like and humic-like components exhibited obvious fluorescence quenching after the heavy metal addition, and the complexes formed with Pb(II) and Zn(II) were more stable. C-H groups exhibited the fastest response to Pb(II) and Zn(II) binding in the FB280 DOM, while the COO- groups of carboxylic acids in the FB380 DOM exhibited the fastest response to Pb(II) and Zn(II). Moreover, the mutation concentration range of components and functional groups in DOM, as analyzed by MW2D-COS, was greater for Zn(II) than for Pb(II). These results provide a more detailed molecular-level understanding of the interaction mechanisms between heavy metals and feces-based biochar-derived DOM and the effect of HM concentration on DOM binding. Further, these results will help to provide a reasonable reference for feces management and feces-based biochar in controlling soil HMs.

关键词： Human feces Biochar Dissolved organic matter Heavy metals binding Moving-window analysis

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Influence of initial microstructure on surface integrity of ultrasonic impacting strengthened 34CrNi3MoA steel crankshaft

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JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY 2024年第2期38卷 683-689页

作者： Chen, Jianzhi Zhuge, Yunhai Ren, Hailiang Jing, xuwen Wang, Yan li, Guochao Jiangsu Univ Sci & Technol Sch Mech Engn Zhenjiang 212003 Peoples R China Henan Diesel Engine Heavy Ind Co Ltd Luoyang 471039 Peoples R China

The ultrasonic impacting process was carried out on the fillet of 34CrNi3MoA steel crankshaft treated by quenching and tempering, medium frequency quenching and gas nitriding respectively, and the variations of surface integrity of crankshaft were analyzed. The results show that the Ra of fillet can be decreased to below 0.2 mu m after the ultrasonic impacting. The ultrasonic impacted crankshaft with initial process of gas nitriding has the highest hardness but the lowest hardness increase rate due to its high deformation resistance of nitriding layer. The ultrasonic impacting can transfer the tensile residual stress to the compressive residual stress on the fillet, the specimens with initial process of gas nitriding exhibit the highest compressive residual stress of -412.17 MPa. The fatigue performance model of the crankshaft was established based on Basquin and Goodman equations, the crankshaft treated by gas nitriding and ultrasonic impacting is supposed to have the highest fatigue limit.

关键词： 34CrNi3MoA steel Crankshaft Fatigue performance Ultrasonic impacting Surface integrity

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Unveiling the relationship between dislocation structure evolution and strain hardening behavior of S32654 super-austenitic stainless steel

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JOURNAL OF MATERIALS SCIENCE 2024年第27期59卷 12715-12731页

作者： liao, Luhai Zhang, He Hu, Shengbo Guo, Rui Yuan, xuwen li, Fengguang Hubei Univ Automot Technol Sch Mat Sci & Engn Shiyan 442002 Peoples R China Wuhan Univ Sci & Technol Key Lab Ferrous Met & Resources Utilizat Minist Educ Wuhan 430081 Peoples R China Wuhan Univ Sci & Technol Hubei Prov Key Lab New Proc Ironmaking & Steel Mak Wuhan 430081 Peoples R China

Understanding the relationship between dislocation structure evolution and strain hardening behavior is crucial for the development of high-strength and high-ductility alloys. In this work, the strain hardening mechanism of S32654 super-austenitic stainless steel is studied by electron backscatter diffraction and transmission electron microscopy. S32654 is characterized by a constant high strain hardening rate accompanied by 835 +/- 10 MPa ultimate tensile strength and 67 +/- 3% elongation. Deformation microstructures at different strain levels are investigated in order to unveil the influence of microstructure on mechanical properties. Research results indicate that although the strain hardening curve of S32654 shows similarities to high-strength twinning-induced plasticity (TWIP) steels, deformation-induced twinning is not the primary strain hardening mechanism for S32654. With increasing deformation, the evolution of dislocation structures in S32654 is mainly dominated by the continuous refinement of slip bands. The spacing between slip bands, as a microstructural parameter, can describe the flow stress curve. The TWIP effect only contributes to the strain hardening of S32654 in the later stages of deformation.

关键词： Strain hardening

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Macrophage-Disguised Manganese Dioxide Nanoparticles for Neuroprotection by Reducing Oxidative Stress and Modulating Inflammatory Microenvironment in Acute Ischemic Stroke

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ADVANCED SCIENCE 2021年第20期8卷 e2101526页

作者： li, Chao Zhao, Zhenhao Luo, Yifan Ning, Tingting liu, Peixin Chen, Qinjun Chu, Yongchao Guo, Qin Zhang, Yiwen Zhou, Wenxi Chen, Hongyi Zhou, Zheng Wang, Yu Su, Boyu You, Haoyu Zhang, Tongyu li, xuwen Song, Haolin li, Chufeng Sun, Tao Jiang, Chen Fudan Univ Sch Pharm Dept PharmaceutState Key Lab Med Neurobiol Key Lab Smart Drug DeliveryMinist EducInst Brai Shanghai 201203 Peoples R China Fudan Univ Sch Pharm Inst Brain Sci MOE Frontiers Ctr Brain SciDept Pharmaceut Shanghai 201203 Peoples R China

Reperfusion injury is still a major challenge that impedes neuronal survival in ischemic stroke. However, the current clinical treatments are remained on single pathological process, which are due to lack of comprehensive neuroprotective effects. Herein, a macrophage-disguised honeycomb manganese dioxide (MnO2) nanosphere loaded with fingolimod (FTY) is developed to salvage the ischemic penumbra. In particular, the biomimetic nanoparticles can accumulate actively in the damaged brain via macrophage-membrane protein-mediated recognition with cell adhesion molecules that are overexpressed on the damaged vascular endothelium. MnO2 nanosphere can consume excess hydrogen peroxide (H2O2) and convert it into desiderated oxygen (O-2), and can be decomposed in acidic lysosome for cargo release, so as to reduce oxidative stress and promote the transition of M1 microglia to M2 type, eventually reversing the proinflammatory microenvironment and reinforcing the survival of damaged neuron. This biomimetic nanomedicine raises new strategy for multitargeted combined treatment of ischemic stroke.

关键词： biomimetic nanoparticles inflammatory microenvironment microglia polarization neuroprotection O-2 generation reperfusion injury reactive oxygen species (ROS) consumption

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Financial inclusion, environmental degradation, and the moderating role of ICT: a global perspective

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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 2023年第1期31卷 445-457页

作者： Khan, Karamat Yan, xuwen Zhang, Jie Ullah, Sami li, Chuntao Henan Univ Sch Econ Kaifeng Peoples R China Zhongnan Univ Econ & Law Sch Finance Wuhan Peoples R China Shandong Univ Res Ctr Labour Econ & Human Resources Weihai Shandong Peoples R China Sias Int Univ Sch Business Zhengzhou Henan Peoples R China

This study aims to investigate the global perspective on the relationship between financial inclusion and environmental degradation, taking into account the potential moderating role of information and communication technology (ICT). The research utilizes panel data from 131 countries, covering the period of 1995 to 2019. The findings show that financial inclusion has significant and positive impact on carbon emissions, implying that as financial inclusion increases, so do carbon emissions. Moreover, our findings reveal a significant negative moderating effect of the ICT on the relationship between financial inclusion and carbon emissions. This implies that the impact of financial inclusion on carbon emissions is contingent upon the level of ICT development. The robustness of these findings is confirmed through the use of alternative proxies for the explanatory and moderating variables, as well as alternative estimation methods. The outcomes of this study carry significant implications for both policy and practice.

关键词： Financial inclusion CO2 emissions ICT GMM Global perspective

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Taxonomy and regeneration of a newly recorded Polychaete Capitella teleta(Annelida,Capitellidae)in the coastal water of Shandong,China

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Journal of Oceanology and limnology 2022年第1期40卷 309-321页

作者： Qian li Yongnan li Yue WANG xuwen WU linlin ZHANG CAS and Shandong Province Key Laboratory of Experimental Marine Biology&Center of Deep Sea Research Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China University of Chinese Academy of Sciences Beijing 100049China Department of Marine Organism Taxonomy&Phylogeny Institute of OceanologyChinese Academy of SciencesQingdao 266071China

The polychaete species of Capitella are widely distributed in the China seas,however little is known about Capitella taxonomy,and specimens collected from China have been identifi ed as Capitella capitata(Fabricius,1780)for more than 50 *** was considered to be Arctic and subarctic in distribution,therefore the records of *** in the China seas probably represent other species.A taxonomic study based on the samples collected from the northeast coastal water of Shandong Province reveals a diff erent species,Capitella teleta Blake et al.,2009,which is recorded in the China seas for the fi rst ***,*** can be easily distinguished from *** by the absence of neuropodial capillaries on chaetigers 8 and *** identity of *** is further supported by genetic distance and phylogenetic analyses assessed from mitochondrial cytochrome c oxidase subunit I(COI)*** addition,the regeneration feature of *** was studied through whole mount immunohistochemistry and chemical *** amputation,the wound of *** was healed within 24 h,forming a signifi cant regeneration blastema by 3 days post amputation(dpa).By 5 dpa,muscle tissues regenerated,nerve fi bers also *** 7 dpa,neurites and muscle tissues are both signifi cantly ***,there are more than ten segments regenerated until 16 *** a highly opportunistic species,Capitella teleta is distributed in China,Japan,Korea,North America,and the *** is expected to be an excellent model for studying developmental genetics and evolution of regeneration.

关键词： Annelid Capitella teleta regeneration Chinese waters

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36例急性高原病胸部CT平扫影像分析

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现代医用影像学 2021年第5期30卷 977-981页

作者：陆良其罗杰李旭文上海市松江区泗泾医院放射科上海201600 定日县人民医院放射科西藏自治区日喀则市858200

目的:研究急性高原病患者胸部CT平扫影像学征象,评估胸部CT平扫检查在AHAD诊断方面的应用价值。方法:选取36例临床诊断为AHAD的患者作为观察组、18例正常进藏旅游者作为对照组,观察组中33例AMAD病例根据CAS评分划分为基本正常AMAD组、轻... 详细信息

目的:研究急性高原病患者胸部CT平扫影像学征象,评估胸部CT平扫检查在AHAD诊断方面的应用价值。方法:选取36例临床诊断为AHAD的患者作为观察组、18例正常进藏旅游者作为对照组,观察组中33例AMAD病例根据CAS评分划分为基本正常AMAD组、轻度AMAD组、中度AMAD组、重度AMAD组;比较观察组与对照组间主肺动脉直径(dPA)、主肺动脉与升主动脉直径比值(rPA)的差异;分析观察组胸部CT平扫影像征象。结果:观察组dPA、rPA值显著高于对照组(P<0.05);观察组3例可见肺水肿影像征象(肺内磨玻璃、云絮、斑片样密度增高影),对照组未见到肺水肿影像征象。结论:胸部CT平扫可以通过测量dPA、计算rPA值为AHAD提供客观诊断依据,通过早期发现肺水肿影像征象为确诊HAPE提供证据,胸部CT平扫在AHAD的诊断及治疗中具有重要作用。

关键词：急性高原病急性轻症高原病高原肺水肿 AHAD AMAD HAPE CT平扫

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Mapping human tissues with highly multiplexed RNA in situ hybridization

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NATURE COMMUNICATIONS 2024年第1期15卷 2511页

作者： Kalhor, Kian Chen, Chien-Ju Lee, Ho Suk Cai, Matthew Nafisi, Mahsa Que, Richard Palmer, Carter R. Yuan, Yixu Zhang, Yida li, xuwen Song, Jinghui Knoten, Amanda Lake, Blue B. Gaut, Joseph P. Keene, C. Dirk Lein, Ed Kharchenko, Peter V. Chun, Jerold Jain, Sanjay Fan, Jian-Bing Zhang, Kun Univ Calif San Diego Dept Bioengn La Jolla CA 92093 USA Univ Calif San Diego Program Bioinformat & Syst Biol La Jolla CA 92093 USA Univ Calif San Diego Dept Elect Engn La Jolla CA USA Sanford Burnham Prebys Med Discovery Inst La Jolla CA USA Univ Calif San Diego Program Biomed Sci La Jolla CA USA Harvard Med Sch Dept Biomed Informat Boston MA USA Altos Labs San Diego CA 94022 USA Washington Univ Sch Med Dept Med St Louis MO USA Washington Univ Sch Med Dept Pathol & Immunol St Louis MO USA Univ Washington Dept Lab Med & Pathol Sch Med Seattle WA USA Allen Inst Brain Sci Seattle WA 98103 USA Illumina San Diego CA USA

In situ transcriptomic techniques promise a holistic view of tissue organization and cell-cell interactions. There has been a surge of multiplexed RNA in situ mapping techniques but their application to human tissues has been limited due to their large size, general lower tissue quality and high autofluorescence. Here we report DART-FISH, a padlock probe-based technology capable of profiling hundreds to thousands of genes in centimeter-sized human tissue sections. We introduce an omni-cell type cytoplasmic stain that substantially improves the segmentation of cell bodies. Our enzyme-free isothermal decoding procedure allows us to image 121 genes in large sections from the human neocortex in <10 h. We successfully recapitulated the cytoarchitecture of 20 neuronal and non-neuronal subclasses. We further performed in situ mapping of 300 genes on a diseased human kidney, profiled >20 healthy and pathological cell states, and identified diseased niches enriched in transcriptionally altered epithelial cells and myofibroblasts.

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Effect of High-Temperature Annealing on Microstructure and Mechanical Properties of Extremely Deformed Fe35Ni35Cr20Mn10 High-Entropy Alloy Wire

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JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE 2025年第1期34卷 885-895页

作者： Shi, Mengchuan liao, Hengcheng Zhou, Jun li, Guangjing Cui, Zhan Yan, Tianrui liu, xuwen Southeast Univ Sch Mat Sci & Engn Jiangsu Key Lab Adv Met Mat Nanjing 211189 Peoples R China Jiangsu Univ Sci & Technol Sch Mat Sci & Engn Zhenjiang 212100 Peoples R China

Fe35Ni35Cr20Mn10 high-entropy alloy wire with a diameter of 0.45 mm, prepared by continuous cold-drawing from a rod with a diameter of 6.34 mm, was subjected to high-temperature annealing at 600, 700, 800, 900, and 1000 degrees C for 1 h. The microstructure and mechanical properties in different states were investigated by microscopy observations and tensile testing. The wire in the as-drawn state exhibited the highest strength but the lowest elongation because of its fine multilayer structure and high dislocation density. Annealing, particularly at higher temperatures, considerably reduced the yield strength and caused significant recovery in both the elongation and strain-hardening exponent. Increasing the annealing temperature significantly decreased the dislocation density, notably coarsened the recrystallized grains, and significantly increased the fraction of twin boundaries and mean widths of annealing twins. The yield strength was found to be positively related to the dislocation density, and the elongation exhibited a negative relationship. In addition to the decrease in dislocation density, the formation of annealing twins played an important role in enhancing the elongation.

关键词： cold drawing high-entropy alloy wire high-temperature annealing mechanical properties microstructure

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