目的探讨鼻中隔偏曲(NSD)对患者中鼻甲(MT)位置的影响及其对鼻中隔成形术的临床意义。方法对2018年2月—2019年12月因NSD行鼻中隔成形术联合MT手术的100例患者的鼻窦CT进行回顾性分析。利用GE医疗图像系统(Centricity Enterprise Web 3...
详细信息
目的探讨鼻中隔偏曲(NSD)对患者中鼻甲(MT)位置的影响及其对鼻中隔成形术的临床意义。方法对2018年2月—2019年12月因NSD行鼻中隔成形术联合MT手术的100例患者的鼻窦CT进行回顾性分析。利用GE医疗图像系统(Centricity Enterprise Web 3)测量NSD的偏曲程度和鼻腔凹、凸侧MT的结构指标,分析MT结构指标与NSD的偏曲程度之间的关系。结果鼻腔凸侧MT宽度(4.56±1.55)mm显著小于凹侧(7.01±1.75)mm(P<0.01),凸侧MT内侧缘距离中线的距离(5.04±1.37)mm显著大于凹侧(1.48±1.50)mm(P<0.01)。MT宽度与NSD的偏曲程度在凹侧呈正相关(r=0.245;P<0.05),在凸侧呈负相关(r=-0.420;P<0.01)。MT到中线的距离与NSD的偏曲程度在凹侧呈负相关(r=-0.430;P<0.01),在凸侧呈正相关(r=0.549;P<0.01)。结论NSD患者凹侧MT代偿性肥大并向中线移位,凸侧MT适应性缩小并远离中线。在鼻中隔成形术中,对包括泡状中鼻甲(CB)在内的肥大MT有必要给予更多关注。
Background and aims The crude extract of the endophyte Paecilomyces variotii known as ZhiNengCong (ZNC) has function of promoting plant growth and enhancing disease resistance and is widely used in China. Our study ai...
详细信息
Background and aims The crude extract of the endophyte Paecilomyces variotii known as ZhiNengCong (ZNC) has function of promoting plant growth and enhancing disease resistance and is widely used in China. Our study aims to evaluate the molecular mechanisms of plant growth promotion and disease protection. Methods We generated transcriptome profiles from ZNC-treated seedlings using RNA sequencing. The function of salicylic acid (SA) in ZNC-mediated immunity was examined using SA biosynthesis and signaling pathway mutants. The concentrations of nitrogen (N) and phosphorus (P) in seedlings under ZNC treatment were measured. The effect of ZNC on the level of the hormone auxin in roots was tested using transgenic plants containing DR5::GFP. Results ZNC exhibited ultrahigh activity in promoting plant growth and enhancing disease resistance, even at concentrations as low as 1-10 ng/ml. ZNC induced ROS accumulation, callose deposition, and expression of PR genes. SA biosynthesis and signaling pathways were required for the ZNC-mediated defense response. Moreover, in improving plant growth, ZNC increased the level of auxin in root tips and regulated the absorption of N and P. Conclusion According to these results, ZNC is a highly effective plant elicitor that promotes plant growth by inducing auxin accumulation at the root tip at low concentrations and enhances plant disease resistance by activating the SA signaling pathway at high concentrations.
A series of cinnamoyl tethered betulinic acid derivatives g1 - g39 was synthesized and evaluated for their inhibitory activity toward α-glucosidase. All the compounds showed excellent inhibition against α-glucosidas...
A series of cinnamoyl tethered betulinic acid derivatives g1 - g39 was synthesized and evaluated for their inhibitory activity toward α-glucosidase. All the compounds showed excellent inhibition against α-glucosidase, in which compound g26 exhibited the most potent α-glucosidase inhibitory activity with an IC 50 value of 1.18 ± 0.12 μM. Inhibition kinetics demonstrated that compound g26 was a non-competitive inhibitor for α-glucosidase. Fluorescence quenching, 3D fluorescence and circular dichroism spectra suggested that compound g26 could cause the conformational changes of α-glucosidase. Molecular docking and molecular dynamics simulation revealed that compound g26 could well interact with the amino acid residues located close to the active sites of α-glucosidase through hydrogen-bonding and hydrophobic interactions. Oral glucose tolerance test showed that compound g26 could improve glucose tolerance. The present results indicated that compound g26 has the potential to become α-glucosidase inhibitor for the treatment of type 2 diabetes.
Androgen receptor (AR), as a member of the nuclear receptor (NR) superfamily, regulates the gene transcription in response to the sequential binding of diverse agonists and coactivators. Great progress has been made i...
详细信息
Androgen receptor (AR), as a member of the nuclear receptor (NR) superfamily, regulates the gene transcription in response to the sequential binding of diverse agonists and coactivators. Great progress has been made in studies on the pharmacology and structure of AR, but the atomic level mechanism of the bidirectional communications between the ligand-binding pocket (LBP) and the activation function-2 (AF2) region of AR remains poorly understood. Therefore, in this study, molecular dynamics (MD) simulations and free energy calculations were carried out to explore the interactions among water, agonist (DHT) or antagonist (HFT), AR, and coactivator (SRC3). Upon the binding of an agonist (DHT) or antagonist (HFT), the LBP structure would transform to the agonistic or antagonistic state, and the conformational changes of the LBP would regulate the structure of the AF2 surface. As a result, the binding of the androgen DHT could promote the recruitment of the coactivator SRC3 to the AF2, and on the contrary, the binding of the antagonist HFT would induce a perturbation to the shape of the AF2 and then weaken its accommodating capability of the coactivators with the LXXLL motif. The simulation results illustrated that the DHT-AR binding affinity was enhanced by the association of the coactivator SRC3, which would reduce the conformational fluctuation of the AR-LBD and expand the size of the AR LBP. On the other hand, the coactivator-to-HFT allosteric pathway, which involves the SRC3, helix 3 (H3), helix 4 (H4), the loop (L1-3) between helix 1 (H1) and H3, and HFT, was characterized. The HFT's skewness and different interactions between HFT and the LBP were observed in the SRC3-present AR The mutual communications between the AF2 surface and LBP, together with the processes involving the interplay of the ligand binding and coactivator recruitment events, would help in understanding the association of coactivators and rationally develop potent drugs to inhibit the activity o
暂无评论