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Histopathological analysis and the immune related gene expression profiles of mandarin fish (Siniperca chuatsi) infected with Aeromonas hydrophila

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FISH & SHELLFISH IMMUNOLOGY 2018年 83卷 410-415页

作者： Chen, Nan Jiang, Jingjing Gao, Xiaojian Li, Xixi Zhang, Yue liu, Xiaodan Yang, Hui Bing, xuwen Zhang, Xiaojun Yangzhou Univ Coll Anim Sci & Technol Yangzhou 225009 Jiangsu Peoples R China Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Key Lab Freshwater Fisheries & Germplasm Utilizat Minist Agr Wuxi 214081 Peoples R China

Hemorrhagic septicemia of mandarin fish (Siniperca chuatsi) was mainly caused by Aeromonas hydrophila which was an opportunistic pathogen. In recent years, the disease has caused tremendous economic loss with high morbidity and mass mortality in the mandarin fish breeding industry. Histopathological analysis and the immune related gene expression profiles of mandarin fish (S. chuatsi) infected with A. hydrophila were investigated in this study. Transmission electron microscopy (TEM) images showed that the cells of A. hydrophila densely covered with a mass of fimbriae. Histopathological analysis revealed that inflammation, vacuolization and extensive necrosis existed in the gill, liver, spleen and head kidney of the diseased fish. Quantitative real-time PCR was performed to measure mRNA expression levels for six immune related genes in mandarin fish after A. hydrophila infection. The transcriptional analysis of these immune related genes demonstrated that the expression levels of major histocompatibility complex class II (MHC II), T cell receptor alpha (TCR alpha), tumor necrosis factor alpha (TNF alpha), CC chemokine 3, interleukin 8 (IL-8) and Hepcidin were strongly up-regulated in spleen and head kidney of mandarin fish post-infection. These results will contribute to further study on the pathogenesis and host defensive system in A. hydrophila infection.

关键词： Mandarin fish Aeromonas hydrophila Histopathology Immune related genes

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球磨工艺对铜基摩擦材料混合料的影响Ⅰ:对粒度分布的影响

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粉末冶金技术 2016年第6期34卷 444-449页

作者：殷德政石增敏张大勇刘旭文三峡大学材料与化工学院湖北宜昌443000 天津瑞克曼德科技发展有限公司天津300050

采用单因素试验方法,研究了行星式球磨工艺参数如球料比、球磨时间、磨球直径、醇料比等对铜基粉末冶金摩擦材料混合料粒度分布的影响规律。结果表明,随球料比的增加,混合料的平均粒径不断减小,球料比为15∶1时,混合料的平均粒径最小,为... 详细信息

采用单因素试验方法,研究了行星式球磨工艺参数如球料比、球磨时间、磨球直径、醇料比等对铜基粉末冶金摩擦材料混合料粒度分布的影响规律。结果表明,随球料比的增加,混合料的平均粒径不断减小,球料比为15∶1时,混合料的平均粒径最小,为1.345μm;球磨时间对混合料平均粒径的影响规律是随着球磨时间的增加混合料平均粒径先减小后增大;一定范围内的醇料比在湿磨过程中对研磨效率影响不大;相比于刚玉磨球,钢质磨球对于混合料的研磨效率更好。

关键词：铜基摩擦材料粉末冶金球磨工艺粒度分布

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The infection of red seabream iridovirus in mandarin fish (Siniperca chuatsi) and the host immune related gene expression profiles

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FISH & SHELLFISH IMMUNOLOGY 2018年 74卷 474-484页

作者： liu, Xiaodan Chen, Nan Gao, Xiaojian Zhang, Yingying Li, Xixi Zhang, Yue Bing, xuwen Huang, Hezhong Zhang, Xiaojun Yangzhou Univ Coll Anim Sci & Technol Yangzhou 225009 Jiangsu Peoples R China Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Minist Agr Key Lab Freshwater Fisheries & Germplasm Utilizat Wuxi 214081 Peoples R China Soochow Univ Fisheries Res Inst Sch Basic Med & Biol Sci Suzhou 215123 Peoples R China

Red Sea bream iridovirus (RSIV) was initially isolated from marine fish, which belongs to Megalocytivirus, Iridoviridae. It can cause great economic losses in fish culture with high morbidity and mortality. In the present study, the pathogenicity and immune response associated with a RSIV genotype megalocytivirus infection were determined in mandarin fish (Siniperca chuatsi). Fish challenged showed typical clinical signs of iridovirus infection, including acute haemorrhages and enlarged visceral organs. Histopathological analysis revealed that extensive necrosis, vacuolization and inflammation were presented in the stomach, spleen, kidney and liver of the diseased fish. Blood cells counting and phagocytic assay indicated that the numbers of the red and white blood cells in the peripheral blood of infected fish increased significantly compared to the control group and the phagocytic percentage of leukocytes peaked at day 6 post infection. Quantitive real-time PCR (qRT-PCR) was also undertaken to analyse the host defensive response in mandarin fish challenged with RSIV. The expression level of ten genes including interferon-related factors (IRFs) IRF1 and IRF7, Mx, Viperin, JAK1, STAT1, TCR alpha, TNF alpha, and IL-8 during experimental infection were monitored at different point of time in liver, spleen and head kidney. Results revealed varying expression profiles and clear transcriptional activation of these immune related genes in different tissues, which will contribute to better understand the pathogenesis and host defensive system in iridovirus invasion.

关键词： Megalocytivirus Red sea bream iridovirus (RSIV) Mandarin fish Histopathological analysis Immune related genes

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Fabrication of CoNPs-embedded porous carbon composites based on morphochemical imprinting strategy for detection of H2O2 released from living cells

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ELECTROCHIMICA ACTA 2019年 321卷

作者： Lu, Nannan Zheng, Bo Gu, Yue Yan, Xiaoyi Zhang, Tingting liu, He Xu, Haixin Xu, Zhiqian Li, xuwen Zhang, Zhiquan Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China Weinan Normal Univ Coll Chem & Mat Weinan City 714099 Peoples R China

Rational construction of biosensor to determine reactive oxygen species (ROS) generated from living cells is one of the great challenges in physiological and pathological fields. Herein, for the first time, a novel Co nanoparticles (CoNPs) embedded walnut-like N-doped porous carbon microsphere (Co@NCS) can be synthesized using dual imprinting strategy by pyrolysis and acid leaching. The distinct morphology and chemical characteristic of melamine cyanurate (MC) aggregates can be imprinted in the carbon source derived from glucose during the pyrolysis process. According to our research, with synergistic effect of CoHPs and NCS, the Co@NCS shows an excellent electrochemical activity toward the reduction of hydrogen peroxide (H2O2). The Co@NCS modified electrode exhibits a wider linear detection response with the range from 0.5 mu M to 7500 mu M and a low detection limit of 88 nM (S/N = 3). Moreover, with desirable selectivity, decent reproducibility and satisfied anti-interference performance, the fabricated biosensor can be applied to determine H2O2 in human serum samples and that released from living cell, indicating the potential applications in the fields of pathological and physiological sensing. (C) 2019 Elsevier Ltd. All rights reserved.

关键词： Co nanoparticles Porous carbon microspheres Biosensor Hydrogen peroxide Living cells

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CELF(CUG-BP,Elav-like family)家族研究进展

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鲁东大学学报（自然科学版） 2017年第3期33卷 231-237页

作者：曹绪文刘学敏冯铄淇杨青云李红岩中国海洋大学海洋生命学院海洋生物多样性与进化研究所山东青岛266003

CELF(CUG-BP,Elav-like family)家族作为RNA结合蛋白广泛存在于动物和植物中,其在细胞核和胞质内调控多个转录后RNA加工过程,包括前体mRNA的选择性剪接、mRNA降解和翻译等.CELF家族在人和其他哺乳动物中有6个成员,分别是:CELF1~6,根据... 详细信息

CELF(CUG-BP,Elav-like family)家族作为RNA结合蛋白广泛存在于动物和植物中,其在细胞核和胞质内调控多个转录后RNA加工过程,包括前体mRNA的选择性剪接、mRNA降解和翻译等.CELF家族在人和其他哺乳动物中有6个成员,分别是:CELF1~6,根据进化关系可分为两个亚家族,CELF1~2和CELF3~6亚家族.CELF介导的转录后调控对早期胚胎发育、配子形成以及肌肉和神经系统功能都很重要.此外,CELF还与强直性肌营养不良、免疫细胞功能、癌症发生等密切相关.本文对CELF家族成员的生物学功能以及CELF对人类疾病影响进行了综述.

关键词： CELF蛋白 RNA结合蛋白强直性肌营养不良癌症

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IK1 channel agonist zacopride suppresses ventricular arrhythmias in conscious rats with healing myocardial infarction

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LIFE SCIENCES 2019年 239卷 117075页

作者： Zhai, xuwen Qiao, Xi Zhang, Li Wang, Dongming Zhang, Lijun Feng, Qilong Wu, Bowei Cao, Jimin liu, Qinghua Shanxi Med Univ Clin Skills Teaching Simulat Hosp Taiyuan Shanxi Peoples R China Shanxi Med Univ Dept Pathophysiol 56 Xinjian South Rd Taiyuan 030001 Shanxi Peoples R China Childrens Hosp Shanxi Clin Lab Taiyuan Shanxi Peoples R China Shanxi Med Univ Hosp 2 Taiyuan Shanxi Peoples R China Shanxi Med Univ Minist Educ Key Lab Cellular Physiol 56 Xinjian South Rd Taiyuan 030001 Shanxi Peoples R China Shanxi Med Univ Dept Physiol 56 Xinjian South Rd Taiyuan 030001 Shanxi Peoples R China

Aims: Arrhythmogenesis of chronic myocardial infarction (MI) is associated with the prolongation of action potential, reduction of inward rectifier potassium (I-K1, Kir) channels and hyper-activity of Calcium/calmodulin-dependent kinase II (CaMKII) in cardiomyocytes. Zacopride, a selective I-K1 agonist, was applied to clarify the cardioprotection of I-K1 agonism via a CaMKII signaling on arrhythmias post-MI. Methods: Male SD rats were implanted wireless transmitter in the abdominal cavity and subjected to left main coronary artery ligation or sham operation. The telemetric ECGs were monitored per day throughout 4 weeks. At the endpoint, isoproterenol (1.28 mg/kg, i.v.) was administered for provocation test. The expressions of Kir2.1 (dominant subunit of I-K1 in ventricle) and CaMKII were detected by Western-blotting. Key findings: In the telemetric rats post-MI, zacopride significantly reduced the episodes of atrioventricular conduction block (AVB), premature ventricular contraction (PVC), ventricular tachycardia (VT) and ventricular fibrillation (VF), without significant effect on superventricular premature contraction (SPVC). In provocation test, zacopride suppressed the onset of ventricular arrhythmias in conscious PMI or sham rats. The expression of Kir2.1 was significantly downregulated and p-CaMKII was upregulated post-MI, whereas both were restored by zacopride treatment. Significance: I-K1/Kir2.1 might be an attractive target for pharmacological controlling of lethal arrhythmias post MI.

关键词： Inward rectifier potassium channel Zacopride Myocardial infarction Arrhythmias Calcium/calmodulin-dependent kinase II

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Amphipathic Side Chain of a Conjugated Polymer Optimizes Dopant Location toward Efficient N-Type Organic Thermoelectrics

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ADVANCED MATERIALS 2021年第4期33卷 e2006694页

作者： liu, Jian Ye, Gang Potgieser, Hinderikus G. O. Koopmans, Marten Sami, Selim Nugraha, Mohamad Insan Villalva, Diego Rosas Sun, Hengda Dong, Jingjin Yang, xuwen Qiu, Xinkai Yao, Chen Portale, Giuseppe Fabiano, Simone Anthopoulos, Thomas D. Baran, Derya Havenith, Remco W. A. Chiechi, Ryan C. Koster, L. Jan Anton Univ Groningen Zernike Inst Adv Mat Nijenborgh 4 NL-9747 AG Groningen Netherlands Univ Groningen Stratingh Inst Chem Nijenborgh 4 NL-9747 AG Groningen Netherlands King Abdullah Univ Sci & Technol KAUST Phys Sci & Engn Div PSE KAUST Solar Ctr KSC Thuwal 239556900 Saudi Arabia Linkoping Univ Dept Sci & Technol Lab Organ Elect SE-60174 Norrkoping Sweden Univ Ghent Dept Inorgan & Phys Chem Krijgslaan 281S3 B-9000 Ghent Belgium

There is no molecular strategy for selectively increasing the Seebeck coefficient without reducing the electrical conductivity for organic thermoelectrics. Here, it is reported that the use of amphipathic side chains in an n-type donor-acceptor copolymer can selectively increase the Seebeck coefficient and thus increase the power factor by a factor of approximate to 5. The amphipathic side chain contains an alkyl chain segment as a spacer between the polymer backbone and an ethylene glycol type chain segment. The use of this alkyl spacer does not only reduce the energetic disorder in the conjugated polymer film but can also properly control the dopant sites away from the backbone, which minimizes the adverse influence of counterions. As confirmed by kinetic Monte Carlo simulations with the host-dopant distance as the only variable, a reduced Coulombic interaction resulting from a larger host-dopant distance contributes to a higher Seebeck coefficient for a given electrical conductivity. Finally, an optimized power factor of 18 mu W m(-1) K-2 is achieved in the doped polymer film. This work provides a facile molecular strategy for selectively improving the Seebeck coefficient and opens up a new route for optimizing the dopant location toward realizing better n-type polymeric thermoelectrics.

关键词： conjugated polymers dopant location N-type doping organic thermoelectrics Seebeck coefficient solution processing

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Parasitic modes suppression in CW cold tests of 1.3 GHz 9-cell high Q cavities at IHEP

arXiv

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arXiv 2021年

作者： Mi, Zhenghui He, Feisi Pan, Weimin Sha, Peng Zhai, Jiyuan Dai, xuwen Jin, Song Zhang, Zhanjun Dong, Chao liu, Baiqi Zhao, Hui Ge, Rui Zhao, Jianbing Mu, Zhihui Du, Lei Sun, Liangrui Zhang, Liang Yang, Conglai Zheng, Xiaobing Lin, Haiying Wang, Guangwei He, Xiangcong Institute of High Energy Physics Chinese Academy of Sciences Beijing100049 China Key Laboratory of Particle Acceleration Physics & Technology Institute of High Energy Physics Chinese Academy of Sciences Beijing100049 China Center for Superconducting RF and Cryogenics Institute of High Energy Physics Chinese Academy of Sciences Beijing100049 China University of Chinese Academy of Sciences Beijing100049 China

The CW RF test of 1.3 GHz 9-cell cavity in liquid helium bath at 2 K is a very important key point in the cavity procurement. Some problems can be found through the test, according which to optimized and improve the process of cavity. Recently, Medium temperature (mid-T) furnace bake of 1.3 GHz 9-cell cavities have been carried out at IHEP. Through the proceed of mid-T bake, the quality factor of cavity has been greatly improved. While the excitation of the parasitic modes in the high Q cavities CW cold test has been encountered, which implies an error source for the cavity gradient and quality factor determination. In order to ensure the testing accuracy of superconducting cavity, we have improved the testing system. Finally, the parasitic mode is completely suppressed and the CW RF cold test of high Q cavity is guaranteed. © 2021, CC BY.

关键词： Superfluid helium

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基于家庭医生签约服务的社区老年2型糖尿病患者居家护理方案构建研究

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哈尔滨医药 2021年第6期41卷 40-42页

作者：郑连花何旭文刘国莲牛萌仇艳敏买娟娟白亚茹宁夏医科大学护理学院宁夏银川750004 三二○一医院陕西汉中723000 宁夏医科大学总医院宁夏银川750004

目的在家庭医生签约服务的基础上,构建一套较为科学、实用的社区老年2型糖尿病患者居家护理方案。方法通过文献回顾、问卷调查、半结构式访谈及专家会议,确定基于家庭医生签约服务的社区老年2型糖尿病患者居家护理方案的框架及具体内容... 详细信息

目的在家庭医生签约服务的基础上,构建一套较为科学、实用的社区老年2型糖尿病患者居家护理方案。方法通过文献回顾、问卷调查、半结构式访谈及专家会议,确定基于家庭医生签约服务的社区老年2型糖尿病患者居家护理方案的框架及具体内容。结果参会专家判断依据系数为0.910,熟悉程度系数为0.820,权威系数为0.865,构建基于家庭医生签约服务的社区老年2型糖尿病患者居家护理方案内容包括居家护理方案的目标和宗旨、居家护理服务团队、居家护理服务流程及内容三部分。结论本研究构建的居家护理方案科学、合理、可靠,可为社区医疗机构开展老年2型糖尿病患者居家护理服务提供依据。

关键词：家庭医生签约服务老年人 2型糖尿病居家护理方案专家会议法

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基于催化臭氧氧化去除煤化工废水中污染物——苯系物

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洁净煤技术 2020年第1期26卷 207-212页

作者：彭思伟何绪文白玉勇谷小兵刘海洋大唐环境产业集团股份有限公司北京100097 中国矿业大学(北京)化学与环境工程学院北京100083

苯、甲苯、乙苯和二甲苯(BTEX)是煤化工废水中典型的难降解有机污染物,通常情况下BTEX较难通过传统的化学氧化技术去除。笔者自主制备了多孔臭氧催化剂,并对催化剂进行表征分析;考察了催化臭氧化降解BTEX的最佳反应条件,并对不同反应体... 详细信息

苯、甲苯、乙苯和二甲苯(BTEX)是煤化工废水中典型的难降解有机污染物,通常情况下BTEX较难通过传统的化学氧化技术去除。笔者自主制备了多孔臭氧催化剂,并对催化剂进行表征分析;考察了催化臭氧化降解BTEX的最佳反应条件,并对不同反应体系中自由基的激发情况进行比对;在此基础上探究催化臭氧化对BTEX的去除机理,为BTEX在实际处理过程中的技术应用提供理论基础。XRD分析结果表明,多孔臭氧催化剂含有氧化铝、氧化硅等,且含有沸石结构的化合物。XPS分析结果表明,所合成的催化剂含Si、O、Cu、Fe、Mn、Al等元素。SEM结果表明,催化剂由许多不规则的细小块状粉末构成,且表面非常蓬松,堆叠成多级结构,使催化剂呈多孔性。比表面积分析表明,催化剂的比表面积为20.8 m^2/g,孔隙直径主要集中在3.8 nm。使用该催化剂对BTEX进行催化臭氧化试验,结果表明,反应温度为30℃、溶液pH=8、臭氧投加量为3.5 mg/L、催化剂投加量为5 g/L时,BTEX的降解效果最佳。在该反应体系中有机物去除率为99.1%,其中苯、甲苯、乙苯、二甲苯的去除率分别为95.6%、98.2%、100%、100%。ESR分析结果表明,催化臭氧化反应体系中羟基自由基和超氧自由基的激发强度明显高于臭氧氧化反应体系,这是因为本文制备的催化剂含有Al、Fe、Mn、Cu氧化物,使催化反应过程中负载的金属氧化物价态间相互变化,转移的电子可促进臭氧分子分解,从而产生更多的自由基。催化臭氧化技术是以羟基自由基为主导,超氧自由基、催化剂吸附为辅助,协同实现煤化工废水中典型有机污染物BTEX的高效去除。

关键词：苯系物催化臭氧化煤化工废水自由基典型有机污染物

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