A novel storage and extraction method was developed for biofluids. Plasma spiked with four types of minor ginsenosides (Rg(2), Rg(3), Rh-1 and Rh-2) was used as a model biofluid to evaluate this method. The method was...
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A novel storage and extraction method was developed for biofluids. Plasma spiked with four types of minor ginsenosides (Rg(2), Rg(3), Rh-1 and Rh-2) was used as a model biofluid to evaluate this method. The method was based on solid-phase adsorption and ultrasonic-assisted nebulization coupled with solid phase extraction (SPA-UANE-SPE). Diatomaceous earth was used as an adsorbent to store the plasma for long periods of time at room temperature. Ultrasonic-assisted nebulization system-solid phase extraction was used to extract the four minor ginsenosides from the plasma. Some experimental parameters, such as the type and amount of the adsorbent, the ultrasonic-assisted nebulization extraction time, the pH of the extraction solution, the concentration of ZnSO4, the amount of C-18, and the type and volume of the eluent were investigated and optimized. Under the optimized conditions, the recoveries of four minor ginsenosides were in the range of 78.5-91.4%, and the RSDs are in the range of 3.5-4.4%. For the storage stability test, the stability of the method was better than that of 4 degrees C storage and was similar to that of -20 degrees C storage. Compared with other methods, the proposed method had a better extraction performance and was advantageous in terms of ease of operation, stable long term storage, and minimal requirements for storage conditions. Thus, this method may be effective for sample preparation in biofluid analysis.
Single walled carbon nanotube network (SWCNTN) was coated by tetrahedral amorphous carbon (ta-C) using a pulsed Filtered Cathodic Vacuum Arc system to form a SWCNTN-ta-C composite film. The effects of SWCNTN areal cov...
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Single walled carbon nanotube network (SWCNTN) was coated by tetrahedral amorphous carbon (ta-C) using a pulsed Filtered Cathodic Vacuum Arc system to form a SWCNTN-ta-C composite film. The effects of SWCNTN areal coverage density and ta-C coating thickness on the composite film properties were investigated. X-Ray photoelectron spectroscopy measurements prove the presence of high quality sp(3) bonded ta-C coating on the SWCNTN. Raman spectroscopy suggests that the single wall carbon nanotubes (SWCNTs) forming the network survived encapsulation in the ta-C coating. Nano-mechanical testing suggests that the ta-C coated SWCNTN has superior wear performance compared to uncoated SWCNTN. (C) 2015 AIP Publishing LLC.
Three hundred gas samples recovered from SHSC-4 during China's first gas hydrate production test in the South China Sea were examined for gas component and isotopic composition. According to the gas chromatography...
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Three hundred gas samples recovered from SHSC-4 during China's first gas hydrate production test in the South China Sea were examined for gas component and isotopic composition. According to the gas chromatography analysis, all the gas samples from SHSC-4 are predominated by CH4, with minor N-2 + O-2, as well as trace amounts of CO2, C2H6, and C3H8. No H2S was detected. The molecular and isotopic data of the gas samples fall into the region of mixed origin on the plot of C-1/(C-2 + C-3) - C-13(1), which is close to the microbial origin. The discrimination diagram of C-13(1) - D-CH4 shows that the methane in all of the samples is of microbial origin, and is derived from the CO2 reduction.
Uterine contraction is one of the most important indication in the labor progression. Electrohysterogram (EHG) is a promising method for monitoring uterine contraction and discriminating efficient and inefficient cont...
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ISBN:
(纸本)9781509028092
Uterine contraction is one of the most important indication in the labor progression. Electrohysterogram (EHG) is a promising method for monitoring uterine contraction and discriminating efficient and inefficient contractions. This study aims to analyze the difference of EHG signals between two groups. EHG signals are recorded with abdominal electrodes from 20 pregnant women, including 10 in term labor group and 10 in non-labor group. Typical linear and nonlinear characteristics of EHG signals, including root mean square (RMS), peak frequency (PF), median frequency (MDF), mean frequency (MNF), parameters from wavelet decomposition (W4, W5) and time reversibility (Tr) are extracted. These characteristics are compared between contraction and non-contraction in term labor group and non-labor group. The result shows that RMS, W4 and W5 of contraction are significantly larger than non-contraction both within term labor group and between two groups (all p<0.001). However, MDF and MNF are significantly smaller (all p<0.05). Furthermore, all characteristics of non-contraction show no significant difference between two groups, except MNF. The variability of RMS, W4, W5 and Tr of contraction are significantly larger than non-contraction both within term labor group and between two groups (all p<0.05, with p<0.001 for W5 and Tr). However, the variability of MDF, PF and MNF are significantly smaller (all p<0.05). Moreover, the variability of all characteristics of non-contractionshows no significant difference between two groups, except MNF. We have shown that characteristics of EHG signals and their variability during contraction are quite different from non-contraction. Therefore, it is feasible to separate uterine contractions and monitor uterine activity with EHG signals.
Combined with the modified damage imaging algorithm based on Reconstruction Algorithm for the Probabilistic Inspection of Damage (RAPID), a baseline-free detection method of the Lamb waves based on time reversal metho...
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Combined with the modified damage imaging algorithm based on Reconstruction Algorithm for the Probabilistic Inspection of Damage (RAPID), a baseline-free detection method of the Lamb waves based on time reversal method is developed to detect the delamination in the composite plate. Through the analysis of the focus position of time reversal signal, a new calculation method of Damage Index (DI) values is proposed. The modified damage imaging algorithm based on RAPID is integrated with two image fusion methods: full summation method and full multiplication method. According to the analysis of limitations of the modified damage imaging algorithm, the transducer array is adopted, and the problem of the uneven probability distribution caused by the uneven density of sensing network is discussed. Modified methods are proposed to eliminate the influence of the uneven probability distribution and improve the accuracy and reliability of the detection results. The parameter significantly affects the effective detection range of each sensing path in the modified damage imaging algorithm. The value of parameter was experimentally determined and reasonably applied to detect the delamination. Based on the calculation of the DI of individual sensing path and the imaging results of delamination, the identification and localization of delamination were experimentally achieved. The imaging results demonstrated the problem of the uneven probability distribution and the validity of modified methods. Copyright (c) 2016 John Wiley & Sons, Ltd.
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