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Elucidating Orbital Delocalization Effects on Boosting Electrochemiluminescence Efficiency of Carbon Nitrides

ADVANCED OPTICAL MATERIALS 2022年第18期10卷

作者： Fang, Yanfeng Hou, Yuhua Yang, Hong Chen, Ran Li, Wang Ma, Jin Han, Dan cao, xuwen Liu, Songqin Shen, Yanfei Zhang, Yuanjian Southeast Univ Jiangsu Engn Lab Smart Carbon Rich Mat & Device Jiangsu Prov Hitech Key Lab Biomed Res State Key Lab BioelectSch Chem & Chem Engn Nanjing 211189 Peoples R China Southeast Univ Med Sch Nanjing 211189 Peoples R China

Highly efficient inter-conversion of different types of energy is the core of science and technology. Among them, electrochemiluminescence (ECL), an emission of light excited by electrochemical reactions, has drawn attention as a powerful tool across diverse fields in addressing global energy, environment, and health challenges. Nonetheless, the ECL efficiency (phi(ECL)) of most luminophores in aqueous solutions is low, significantly hampering their broad applications. Along this line, developing ECL luminophores with high phi(ECL) and understanding the associated intrinsic factors is highly envisioned. Herein, taking carbon nitride (CN) with rigid 2D backbones as an emerging model luminophore, it is reported that the orbital delocalization is a unified and quantifiable factor for its phi(ECL). Behind the complicated transformation of molecular structures of cyano-terminal groups and triazine/heptazine basal frameworks, the orbital delocalization of CN is found to be generally improved at an elevated condensation temperature. Such intrinsic evolution in electronic structure favored the electron injection in excitation and follow-up photon emission in ECL for CN. As a result, the cathodic phi(ECL) of CN is remarkably improved to a new milestone of 24-fold greater than the previous record.

关键词： carbon nitrides electrochemiluminescence energy conversion efficiency orbital delocalization photoelectrochemistry

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Quantitative evaluation of O2 activation half-reaction for Fe-N-C in oxidase-like activity enhancement

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CATALYSIS SCIENCE & TECHNOLOGY 2021年第22期11卷 7255-7259页

作者： Chen, Xinghua Zhu, Caixia Xu, Yuan Wang, Kaiyuan cao, xuwen Shen, Yanfei Liu, Songqin Zhang, Yuanjian Southeast Univ Sch Chem & Chem Engn Jiangsu Engn Lab Smart Carbon Rich Mat & Device Jiangsu Prov Hitech Key Lab Biomed ResState Key Nanjing 211189 Peoples R China Southeast Univ Med Sch Nanjing 210009 Peoples R China

The oxidase-like activity of high-performance Fe-N-C was enhanced by up to 55% by using a secondary N-containing precursor in pyrolysis. Moreover, the exploration of the O-2 activation half-reaction using electrochemical methods was disclosed to be a practically facile approach to quantitatively evaluate the factors associated with the activity.

关键词： Iron compounds

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EAT-2 attenuates C. elegans development via metabolic remodeling in a chemically defined food environment

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CELLULAR AND MOLECULAR LIFE SCIENCES 2023年第8期80卷 205-205页

作者： cao, xuwen Xie, Yusu Yang, Hanwen Sun, Peiqi Xue, Beining Garcia, L. Rene Zhang, Liusuo Chinese Acad Sci Inst Oceanol CAS & Shandong Prov Key Lab Expt Marine Biol Qingdao 266071 Peoples R China Qingdao Natl Lab Marine Sci & Technol Lab Marine Biol & Biotechnol Qingdao 266237 Peoples R China Chinese Acad Sci Ctr Ocean Mega Sci 7 Nanhai Rd Qingdao 266071 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Texas A&M Univ Dept Biol College Stn TX 77843 USA Shandong Univ Inst Marine Sci & Technol 72 Binhai Rd Qingdao 266237 Peoples R China

Dietary intake and nutrient composition regulate animal growth and development;however, the underlying mechanisms remain elusive. Our previous study has shown that either the mammalian deafness homolog gene tmc-1 or its downstream acetylcholine receptor gene eat-2 attenuates Caenorhabditis elegans development in a chemically defined food CeMM (C. elegans maintenance medium) environment, but the underpinning mechanisms are not well-understood. Here, we found that, in CeMM food environment, for both eat-2 and tmc-1 fast-growing mutants, several fatty acid synthesis and elongation genes were highly expressed, while many fatty acid beta-oxidation genes were repressed. Accordingly, dietary supplementation of individual fatty acids, such as monomethyl branch chain fatty acid C17ISO, palmitic acid and stearic acid significantly promoted wild-type animal development on CeMM, and mutations in either C17ISO synthesis gene elo-5 or elo-6 slowed the rapid growth of eat-2 mutant. Tissue-specific rescue experiments showed that elo-6 promoted animal development mainly in the intestine. Furthermore, transcriptome and metabolome analyses revealed that elo-6/C17ISO regulation of C. elegans development may be correlated with up-regulating expression of cuticle synthetic and hedgehog signaling genes, as well as promoting biosynthesis of amino acids, amino acid derivatives and vitamins. Correspondingly, we found that amino acid derivative S-adenosylmethionine and its upstream metabolite methionine sulfoxide significantly promoted C. elegans development on CeMM. This study demonstrated that C17ISO, palmitic acid, stearic acid, S-adenosylmethionine and methionine sulfoxide inhibited or bypassed the TMC-1 and EAT-2-mediated attenuation of development via metabolic remodeling, and allowed the animals to adapt to the new nutritional niche.

关键词： C. elegans maintenance medium CeMM Development Acetylcholine receptor Fatty acid C17ISO S-adenosylmethionine SAM

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“新丝绸之路经济带”背景下国家物流绩效对我国农产品进口的影响测度

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商业经济研究 2021年第16期 163-166页

作者：曹蓄温中共重庆市万州区委党校重庆404000

随着“新丝绸之路经济带”建设步伐的加快,我国农产品的进口重点逐渐由欧美转向丝路沿线诸国。近年来我国在丝路沿线各国的农产品进口额逐年快速增长,但与持续增长的农产品进口额相比,我国在丝路沿线的物流水平提升相对迟缓,故而物流绩... 详细信息

随着“新丝绸之路经济带”建设步伐的加快,我国农产品的进口重点逐渐由欧美转向丝路沿线诸国。近年来我国在丝路沿线各国的农产品进口额逐年快速增长,但与持续增长的农产品进口额相比,我国在丝路沿线的物流水平提升相对迟缓,故而物流绩效日益成为影响我国农产品进口的重要因素。因此,本文选取我国在2008-2019年间与丝路沿线20个农产品贸易国之间的进口数据作为研究样本,构建回归方程对物流绩效如何影响我国农产品进口进行了测度,旨在明确物流绩效的影响机制以促进我国农产品进口贸易的发展。结果表明:“新丝绸之路经济带”中大多数国家的国际物流水平发展不平衡且质量不高,总体来看尚处于上升阶段;国家物流绩效指数对于农产品进口贸易有着显著影响,且国家物流绩效指数下各分项指标对农产品进口贸易的影响有着显著的差异性,其中物流基础设施建设的程度对其影响最大。

关键词：物流绩效农产品进口 “新丝绸之路经济带” 引力模型物流水平

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Effect of planting salt-tolerant legumes on coastal saline soil nutrient availability and microbial communities

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JOURNAL OF ENVIRONMENTAL MANAGEMENT 2023年第1期345卷 118574页

作者： Zheng, Yanfen cao, xuwen Zhou, Yanan Li, Zhe Yang, Yanzhe Zhao, Donglin Li, Yiqiang Xu, Zongchang Zhang, Cheng-Sheng Chinese Acad Agr Sci Tobacco Res Inst Marine Agr Res Ctr Qingdao 266101 Peoples R China Shandong Univ Inst Marine Sci & Technol Qingdao 266200 Peoples R China Natl Ctr Technol Innovat Comprehens Utilizat Salin Dongying 257300 Peoples R China

Soil salinization is a serious global environmental problem affecting sustainable development of agriculture. Legumes are excellent candidates for the phytoremediation of saline soils;however, how soil microbes mediate the amelioration of coastal saline ecosystems is unknown. In this study, two salt-tolerant legumes, Glycine soja and Sesbania cannabina were planted in coastal saline soil for three years. Soil nutrient availability and microbiota structure (including bacteria, fungi, and diazotrophs) were compared between the phytoremediated soils and control soil (barren land). Planting legumes reduced soil salinity, and increased total carbon, total nitrogen, and NO3  -N contents. Among the soil microbiota, some nitrogen-fixing bacteria (e.g., Azotobacter) were enriched in legumes, which were probably responsible for soil nitrogen accumulation. The complexity of the bacterial, fungal, and diazotrophic networks increased significantly from the control to the phytoremediated soils, suggesting that the soil microbial community formed closer ecological interactions during remediation. Furthermore, the dominant microbial functions were chemoheterotrophy (24.75%) and aerobic chemoheterotrophy (21.97%) involved in the carbon cycle, followed by nitrification (13.68%) and aerobic ammonia oxidation (13.34%) involved in the nitrogen cycle. Overall, our findings suggested that G. soja and S. cannabina legumes were suitable for ameliorating saline soils as they decreased soil salinity and increased soil nutrient content, with microorganisms especially nitrogen-fixing bacteria, playing an important role in this remediation process.

关键词： Phytoremediation Saline soil Nitrogen Microbial community Salt-tolerant legume

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东非鲁伍马盆地下始新统深水沉积储层特征及沉积演化

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海相油气地质 2022年第4期27卷 396-404页

作者：曹全斌鲁银涛陈宇航王瑞峰曹旭文马宏霞庞旭中国石油杭州地质研究院中国科学院海洋地质与环境重点实验室西安石油大学中国石油勘探开发研究院国家海洋局第二海洋研究所

针对东非鲁伍马盆地深水沉积体系演化的复杂性,基于地震资料和钻井资料等资料,对古近系下始新统深水沉积特征及深水沉积体系演化期次进行分析研究。结果表明:下始新统深水沉积体系发育碎屑岩和碳酸盐岩两种岩石类型,天然气主要分布在泥... 详细信息

针对东非鲁伍马盆地深水沉积体系演化的复杂性,基于地震资料和钻井资料等资料,对古近系下始新统深水沉积特征及深水沉积体系演化期次进行分析研究。结果表明:下始新统深水沉积体系发育碎屑岩和碳酸盐岩两种岩石类型,天然气主要分布在泥质含量低、颗粒分选程度高的富砂岩相中;储层成岩作用不强,孔隙类型以原生粒间孔为主;岩相和岩石结构成熟度是影响储层质量的主要因素。下始新统深水沉积体系可以进一步细分为5期沉积。受重力流和底流共同作用,深水沉积体系总体表现为由北向南迁移的特点,主要为水道沉积,晚期受海底底形影响演化为朵体沉积。认为研究区内4口天然气发现井的储层属于3个不同期次的深水沉积砂体,气藏评价需考虑3个气藏砂体之间的连通性。

关键词：深水沉积沉积演化沉积模式鲁伍马盆地东非

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Giants among Cnidaria: Large Nuclear Genomes and Rearranged Mitochondrial Genomes in Siphonophores

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GENOME BIOLOGY AND EVOLUTION 2024年第3期16卷 evae048页

作者： Ahuja, Namrata cao, xuwen Schultz, Darrin T. Picciani, Natasha Lord, Arianna Shao, Shengyuan Jia, Kejue Burdick, David R. Haddock, Steven H. D. Li, Yuanning Dunn, Casey W. Yale Univ Dept Ecol & Evolutionary Biol New Haven CT 06520 USA Shandong Univ Inst Marine Sci & Technol Qingdao 266237 Peoples R China Univ Vienna Dept Neurosci & Dev Biol A-1010 Vienna Austria Harvard Univ Dept Organism & Evolutionary Biol Museum Comparat Zool Cambridge MA USA Yale Univ Dept Mol Cellular & Dev Biol New Haven CT USA Univ Guam Marine Lab Mangilao GU USA Monterey Bay Aquarium Res Inst Moss Landing CA USA

Siphonophores (Cnidaria: Hydrozoa) are abundant predators found throughout the ocean and are important constituents of the global zooplankton community. They range in length from a few centimeters to tens of meters. They are gelatinous, fragile, and difficult to collect, so many aspects of the biology of these roughly 200 species remain poorly understood. To survey siphonophore genome diversity, we performed Illumina sequencing of 32 species sampled broadly across the phylogeny. Sequencing depth was sufficient to estimate nuclear genome size from k-mer spectra in six specimens, ranging from 0.7 to 2.3 Gb, with heterozygosity estimates between 0.69% and 2.32%. Incremental k-mer counting indicates k-mer peaks can be absent with nearly 20x read coverage, suggesting minimum genome sizes range from 1.4 to 5.6 Gb in the 25 samples without peaks in the k-mer spectra. This work confirms most siphonophore nuclear genomes are large relative to the genomes of other cnidarians, but also identifies several with reduced size that are tractable targets for future siphonophore nuclear genome assembly projects. We also assembled complete mitochondrial genomes for 33 specimens from these new data, indicating a conserved gene order shared among nonsiphonophore hydrozoans, Cystonectae, and some Physonectae, revealing the ancestral mitochondrial gene order of siphonophores. Our results also suggest extensive rearrangement of mitochondrial genomes within other Physonectae and in Calycophorae. Though siphonophores comprise a small fraction of cnidarian species, this survey greatly expands our understanding of cnidarian genome diversity. This study further illustrates both the importance of deep phylogenetic sampling and the utility of k-mer-based genome skimming in understanding the genomic diversity of a clade.

关键词： k-mer spectra genome skimming genome size mitochondrial genomes

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Purines enrich root-associated Pseudomonas and improve wild soybean growth under salt stress

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NATURE COMMUNICATIONS 2024年第1期15卷 3520页

作者： Zheng, Yanfen cao, xuwen Zhou, Yanan Ma, Siqi Wang, Youqiang Li, Zhe Zhao, Donglin Yang, Yanzhe Zhang, Han Meng, Chen Xie, Zhihong Sui, Xiaona Xu, Kangwen Li, Yiqiang Zhang, Cheng-Sheng Chinese Acad Agr Sci Tobacco Res Inst Marine Agr Res Ctr Qingdao 266101 Peoples R China Shandong Univ Inst Marine Sci & Technol Qingdao 266200 Peoples R China Shandong Agr Univ Coll Resources & Environm Natl Engn Lab Efficient Utilizat Soil & Fertilizer Tai An 271018 Peoples R China

The root-associated microbiota plays an important role in the response to environmental stress. However, the underlying mechanisms controlling the interaction between salt-stressed plants and microbiota are poorly understood. Here, by focusing on a salt-tolerant plant wild soybean (Glycine soja), we demonstrate that highly conserved microbes dominated by Pseudomonas are enriched in the root and rhizosphere microbiota of salt-stressed plant. Two corresponding Pseudomonas isolates are confirmed to enhance the salt tolerance of wild soybean. Shotgun metagenomic and metatranscriptomic sequencing reveal that motility-associated genes, mainly chemotaxis and flagellar assembly, are significantly enriched and expressed in salt-treated samples. We further find that roots of salt stressed plants secreted purines, especially xanthine, which induce motility of the Pseudomonas isolates. Moreover, exogenous application for xanthine to non-stressed plants results in Pseudomonas enrichment, reproducing the microbiota shift in salt-stressed root. Finally, Pseudomonas mutant analysis shows that the motility related gene cheW is required for chemotaxis toward xanthine and for enhancing plant salt tolerance. Our study proposes that wild soybean recruits beneficial Pseudomonas species by exudating key metabolites (i.e., purine) against salt stress.

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Gill and brain transcriptomic analysis of mandarin fish(Siniperca chuatsi) reveals hypoxia-induced mitochondrial dysfunction and modulation of metabolism

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COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 2025年 53卷 101367页

作者： Ding, Weidong cao, Liping cao, Zheming Bing, xuwen Chinese Acad Fishery Sci Minist Agr Freshwater Fisheries Res Ctr Key Lab Freshwater Fisheries & Germplasm Resources Wuxi 214081 Peoples R China

The oxygen content in the fish ponds is facing greater challenges than before in the aquaculture of mandarin fish (Siniperca chuatsi) due to the change of climate and eutrophication. Until now, little is known about the molecular mechanisms underlying the harmful effects of hypoxia on this species. In this work, we built transcriptomes for the mandarin fish that were exposed to decreased oxygen concentration at two times points (24 hand 96 h). The respiratory metabolism activities of pyruvate kinase (PK), hexokinase (HK), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) had different significantly changes during hypoxic treatment. Histological observation of the gill and brain also revealed some damages by hypoxia. A total of 196,355 transcripts were involved in the Gene Ontology analysis, and the numbers of differentially expressed genes (DEGs) in the brain and the gill between the control and experiment groups are 141 and 552 respectively involved in the different hypoxic stress time. The DEGs were then analyzed using KEGG enrichment analysis. The results showed significant differences in the expression of some genes involved in ribosome pathways,biosynthesis of amino acids, hippo signaling pathway, and pentose phosphate pathway,glycolysis/gluconeogenesis pathway and the TCA cycle. The huge number of transcriptome sequences collected in this study has enhanced the mandarin fish gene resources, and the identified DEGs and related pathway analysis give essential information for understanding biological responses to hypoxia.

关键词： Mandarin fish Transcriptome Hypoxia DEGs

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社交媒体在突发事件报道中的传播研究

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湘南学院学报 2020年第3期41卷 53-56页

作者：曹栩雯湖南大学新闻传播与影视艺术学院湖南长沙410000

社交媒体利用其传播速度快、受众范围广、信息新鲜等特点,在一些重大突发事件的报道中发挥了重要作用,成为突发事件报道的重要平台。社交媒体的即时性加速了信息的传播,让受众得到一手资讯,并赋予受众互动的权利。探讨了社交媒体在突发... 详细信息

社交媒体利用其传播速度快、受众范围广、信息新鲜等特点,在一些重大突发事件的报道中发挥了重要作用,成为突发事件报道的重要平台。社交媒体的即时性加速了信息的传播,让受众得到一手资讯,并赋予受众互动的权利。探讨了社交媒体在突发事件报道中的应用及其所带来的问题。

关键词：时效性舆论引导互动新闻失实人文关怀专业性

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