To utilize wave energy, we propose a power take-off device mainly composed of a novel buoy and a high-temperature superconducting synchronous reluctance linear generator (HTS-SRLG). We describe the conceptual structur...
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To utilize wave energy, we propose a power take-off device mainly composed of a novel buoy and a high-temperature superconducting synchronous reluctance linear generator (HTS-SRLG). We describe the conceptual structure and function of the buoy, which makes use of seawater to provide more power to the HTS-SRLG. Then, we introduce the configuration and the key electrical design process of the HTS-SRLG. Design result of the HTS-SRLG is presented and compared with that of counterparts to demonstrate its advantages in performance and in application. This article is conducted from the viewpoint of promoting the industrial application of HTS machines. The proposed buoy gives an insight of improving the utilizing efficiency of wave energy. And the HTS-SRLG contributes to simplify the generator cooling.
The processing quality of the block hole system affects the working performance of the marine diesel engine block directly. Choosing an appropriate combination of process parameters is a prerequisite to improve the ac...
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Somatic embryogenesis (SE) is a critical transition from vegetative to embryogenic growth in higher plants;however, few studies have investigated the mechanism that regulates SE initial differentiation. Most cotton va...
Somatic embryogenesis (SE) is a critical transition from vegetative to embryogenic growth in higher plants;however, few studies have investigated the mechanism that regulates SE initial differentiation. Most cotton varieties have not undergone regeneration by SE, so only a few varieties can be used in genetic engineering. Here, two varieties of cotton with different SE capabilities (HD, higher differentiation and LD, lower differentiation) were analyzed by high throughout RNA-Seq at the preinduction stage (0h) and two induction stages (3h and 3d) under callus-induction medium (CIM). About 1150 million clean reads were obtained from 98.21% raw data. Transcriptomic analysis revealed that "protein kinase activity" and "oxidoreductase activity" were highly represented GO terms during the same and different treatment stages among HD and LD. Moreover, several stress-related transcription factors might play important roles in SE initiation. The SE-related regulation genes (SERKs) showed different expression patterns between HD and LD. Furthermore, the complex auxin and ethylene signaling pathway contributes to initiation of differentiation in SE. Thus, our RNA-sequencing of comparative transcriptome analysis will lay a foundation for future studies to better define early somatic formation in cotton with different SE capabilities.
As the most abundant internal mRNA modification, N6-methyladenosine (m6A) RNA methylation has been found to influence many biological events including bone mesenchymal stem cells (BMSCs) osteogenic differentiation. YT...
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As the most abundant internal mRNA modification, N6-methyladenosine (m6A) RNA methylation has been found to influence many biological events including bone mesenchymal stem cells (BMSCs) osteogenic differentiation. YTH N6-methyladenosine RNA binding protein C2 (YTHDC2) is an m6A reading protein with the ability to mediate the decay of combined methylated mRNA, however its role in BMSCs osteogenic differentiation remains unknown. In this study, we first found an increase of RUNX family transcription factor 2 (RUNX2) expression and a decrease of YTHDC2 expression during the process of BMSCs osteogenic dif-ferentiation. Furthermore, we transfected BMSCs with YTHDC2 interference fragment, resulting in an increased content of RUNX2 mRNA and protein inside BMSCs. Finally, through RNA Immunoprecipitation experiments, we confirmed that YTHDC2 protein can bind to RUNX2 mRNA and accelerate its decomposition. Moreover, the immunofluorescence staining also showed a negative correlation between YTHDC2 and RUNX2. In conclusion, during BMSCs osteogenic differentiation, YTHDC2 protein showed decreased expression, resulting in a higher level of RUNX2 (mRNA and protein) expression inside cells, indicating YTHDC2 as a promising molecular target for the regulation of BMSCs osteogenic differentiation.
In the process design and reuse of marine component products, there are a lot of heterogeneous models, causing the problem that the process knowledge and process design experience contained in them are difficult to ex...
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