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环境工程技术学报 2019年第6期9卷 666-672页

作者：孙艺欣曹刚戴景富张春宇沈志强宋玉栋何绪文周岳溪中国矿业大学(北京)化学与环境工程学院北京100083 中国环境科学研究院水污染控制技术研究中心北京100012 环境基准与风险评估国家重点实验室中国环境科学研究院北京100012 中国石油天然气股份有限公司吉林石化分公司吉林吉林132022

聚丙烯酸钠对阴离子交换膜的污染是影响双极膜电渗析回收丙烯酸丁酯废水中有机酸的关键因素之一。针对该问题研究开发了聚丙烯酸钠污染后离子交换膜的清洗方法,并采用膜电阻、迁移数、表面X射线光电子谱(XPS)分析等对膜清洗效果进行了... 详细信息

聚丙烯酸钠对阴离子交换膜的污染是影响双极膜电渗析回收丙烯酸丁酯废水中有机酸的关键因素之一。针对该问题研究开发了聚丙烯酸钠污染后离子交换膜的清洗方法,并采用膜电阻、迁移数、表面X射线光电子谱(XPS)分析等对膜清洗效果进行了表征。NaOH、NaCl和HCl溶液3种清洗液对离子交换膜的机械清洗及超声强化清洗效果表明,HCl溶液对聚丙烯酸钠污染阴离子交换膜的清洗效果最好,超声波可促进NaOH、NaCl和HCl溶液的清洗效果。采用2.0 mol L的HCl溶液对受污染阴离子交换膜进行超声清洗,其膜电阻和迁移数可基本恢复到未污染膜的水平。

关键词：聚丙烯酸钠离子交换膜污染膜清洗超声强化清洗

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Data Report: Molecular and Isotopic Compositions of the Extracted Gas from China's First Offshore Natural Gas Hydrate Production Test in South China Sea

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ENERGIES 2018年第10期11卷 2793-2793页

作者： Ye, Jianliang Qin, xuwen Qiu, Haijun Xie, Wenwei Lu, Hongfeng Lu, Cheng Zhou, Jianhou Liu, Jiyong Yang, Tianbang cao, Jun Sa, Rina China Geol Survey Guangzhou Marine Geol Survey Guangzhou 510760 Guangdong Peoples R China

Three hundred gas samples recovered from SHSC-4 during China's first gas hydrate production test in the South China Sea were examined for gas component and isotopic composition. According to the gas chromatography analysis, all the gas samples from SHSC-4 are predominated by CH4, with minor N-2 + O-2, as well as trace amounts of CO2, C2H6, and C3H8. No H2S was detected. The molecular and isotopic data of the gas samples fall into the region of mixed origin on the plot of C-1/(C-2 + C-3) - C-13(1), which is close to the microbial origin. The discrimination diagram of C-13(1) - D-CH4 shows that the methane in all of the samples is of microbial origin, and is derived from the CO2 reduction.

关键词： isotopic composition methane gas hydrate South China Sea

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Identification and characterization of a LTR retrotransposon from the genome of Cyprinus carpio var. Jian

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GENETICA 2016年第3期144卷 325-333页

作者： cao, Liping Yin, Guojun cao, Zheming Bing, xuwen Ding, Weidong Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Minist Agr Key Lab Freshwater Fisheries & Germplasm Resource Wuxi 214081 Peoples R China

A Ty3/gypsy-retrotransposon-type transposon was found in the genome of the Jian carp (Cyprinus carpio var. Jian) in a previous study (unpublished), and was designated a JRE retrotransposon (Jian retrotransposon). The full-length JRE retrotransposon is 5126 bp, which includes two long terminal repeats of 470 bp at the 5' end and 453 bp at the 3' end, and two open reading frames between them: 4203 bp encoding the group-specific antigen (GAG) and polyprotein (POL). The pol gene has a typical Ty3/gypsy retrotransposon structure, and the gene order is protease, reverse transcriptase, RNase H, and integrase (PR-RT-RH-IN). A phylogenetic analysis of the pol gene showed that it has similarities of 40.7, 40, and 32.8 %, to retrotransposons of Azumapecten farreri, Mizuhopecten yessoensis, and Xiphophorus maculatus, respectively. Therefore, JRE might belong to the JULE retrotransposon family. The copy number of the JRE transposon in the genome of the Jian carp is 124, determined with real-time quantitative PCR. The mRNA of the JRE retrotransposon is expressed in five Jian carp tissues, the liver, kidney, blood, muscle, and gonad, and slightly higher in the kidney and liver than in the other tissues.

关键词： Cyprinus carpio var. Jian Retrotransposon Copy number Fluorescent in situ hybridization

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中国城镇化发展缘何未能促进城乡居民消费同步增长?——基于省际面板数据的实证研究

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南京社会科学 2017年第9期 20-27,44页

作者：齐红倩席旭文曹莉吉林大学数量经济研究中心暨商学院吉林大学商学院

缩小城乡收入差距、扩大农村消费是城镇化的重要目标。但在过去30多年中,中国出现了城镇化快速发展和城乡居民消费差距扩大并存的现象。对此,本文在理论分析城镇化对城乡居民消费影响机理的基础上,运用2002-2015年中国省际面板数据,通... 详细信息

缩小城乡收入差距、扩大农村消费是城镇化的重要目标。但在过去30多年中,中国出现了城镇化快速发展和城乡居民消费差距扩大并存的现象。对此,本文在理论分析城镇化对城乡居民消费影响机理的基础上,运用2002-2015年中国省际面板数据,通过建立PVAR模型实证检验城镇化发展对城乡消费增长的影响,发现中国"城市偏向"的城镇化发展模式通过提升城市居民收入水平、强化农村居民预防性储蓄动机以及引致农业转移人口家庭福利损失等途径抑制了城乡消费的同步增长。基于PSTR模型的进一步研究表明,城镇化对城乡消费水平的影响效果随着收入的增加呈现出较为平坦的"S"型特征,城市和农村地区进入影响显著区制的收入"门限"分别为7778元和5367元,但城市地区达到"门限"值的时间远远早于农村地区。

关键词：城镇化城乡差距消费 PVAR模型 PSTR模型

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Comparative Transcriptome Analysis of SE initial dedifferentiation in cotton of different SE capability

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SCIENTIFIC REPORTS 2017年第1期7卷 8583-8583页

作者： cao, Aiping Zheng, Yinying Yu, Yu Wang, xuwen Shao, Dongnan Sun, Jie Cui, Baiming Shihezi Univ Key Lab Oasis Ecoagr Coll Agr Shihezi Peoples R China Shihezi Univ Coll Life Sci Shihezi Peoples R China XinJiang Acad Agr & Reclamat Sci Cotton Res Inst Shihezi Peoples R China

Somatic embryogenesis (SE) is a critical transition from vegetative to embryogenic growth in higher plants;however, few studies have investigated the mechanism that regulates SE initial differentiation. Most cotton varieties have not undergone regeneration by SE, so only a few varieties can be used in genetic engineering. Here, two varieties of cotton with different SE capabilities (HD, higher differentiation and LD, lower differentiation) were analyzed by high throughout RNA-Seq at the preinduction stage (0h) and two induction stages (3h and 3d) under callus-induction medium (CIM). About 1150 million clean reads were obtained from 98.21% raw data. Transcriptomic analysis revealed that "protein kinase activity" and "oxidoreductase activity" were highly represented GO terms during the same and different treatment stages among HD and LD. Moreover, several stress-related transcription factors might play important roles in SE initiation. The SE-related regulation genes (SERKs) showed different expression patterns between HD and LD. Furthermore, the complex auxin and ethylene signaling pathway contributes to initiation of differentiation in SE. Thus, our RNA-sequencing of comparative transcriptome analysis will lay a foundation for future studies to better define early somatic formation in cotton with different SE capabilities.

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黄鳝粘液雌雄差异的cDNA-SRAP分析

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华中农业大学学报 2017年第3期36卷 74-78页

作者：胡彬曹哲明丁炜东邴旭文南京农业大学无锡渔业学院无锡214182 中国水产科学研究院淡水渔业研究中心无锡214081

采用cDNA-SRAP技术对黄鳝雌雄粘液进行基因差异表达分析,筛选黄鳝体表粘液中与性别相关的分子标记。从64对引物组合中筛选出12对引物组合,共获得123个稳定清晰的扩增位点,并筛选出4条雌雄有差异的条带。这4条特异性条带经过回收、克隆... 详细信息

采用cDNA-SRAP技术对黄鳝雌雄粘液进行基因差异表达分析,筛选黄鳝体表粘液中与性别相关的分子标记。从64对引物组合中筛选出12对引物组合,共获得123个稳定清晰的扩增位点,并筛选出4条雌雄有差异的条带。这4条特异性条带经过回收、克隆、测序后,将测序结果进行Blast分析,结果发现在GenBank中只有片段1与白鳍豚硫酸盐/硫代硫酸盐CYSA样进口ATP结合盒蛋白有较高的同源性。根据片段1的序列设计1对引物进行半定量RT-PCR反应,结果发现,片段1在雌雄黄鳝体表粘液均可获得扩增条带,但其在雄性粘液中的表达量显著高于雌性。这可能是由于雌雄黄鳝粘液中所存在的表皮细胞和粘液腺细胞有差异所致。

关键词：黄鳝粘液 cDNA-SRAP技术性别特异分子标记

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CEPC Technical Design Report - Accelerator (v2)

arXiv

引用

arXiv 2023年

作者： Abdallah, Waleed De Freitas, Tiago Carlos Adorno Afanaciev, Konstantin Ahmad, Shakeel Ahmed, Ijaz Ai, Xiaocong Aleem, Abid Altmannshofer, Wolfgang Alves, Fabio An, Weiming An, Rui Anderle, Daniele Paolo Antusch, Stefan Arai, Yasuo Arbuzov, Andrej Arhrib, Abdesslam Ashry, Mustafa Bai, Sha Bai, Yu Bai, Yang Bairathi, Vipul Balazs, Csaba Bambade, Philip Ban, Yong Bandyopadhyay, Triparno Bao, Shou-Shan Barber, Desmond P. Bat, Ayse Batozskaya, Varvara Behera, Subash Chandra Belyaev, Alexander Bertucci, Michele Bi, Xiao-Jun Bi, Yuanjie Bian, Tianjian Bianchi, Fabrizio Biekötter, Thomas Biglietti, Michela Bilanishvili, Shalva Binglin, Deng Bodrov, Denis Bogomyagkov, Anton Bondarenko, Serge Boogert, Stewart Boonekamp, Maarten Borri, Marcello Bosotti, Angelo Boudry, Vincent Boukidi, Mohammed Boyko, Igor Bozovic, Ivanka Bozzi, Giuseppe Brient, Jean-Claude Budzinskaya, Anastasiia Bukhari, Masroor Bytev, Vladimir Cacciapaglia, Giacomo Cai, Hua Cai, Wenyong Cai, Wujun Cai, Yijian Cai, Yizhou Cai, Yuchen Cai, Haiying Cai, Huacheng Calibbi, Lorenzo Cang, Junsong cao, Guofu cao, Jianshe Chance, Antoine Chang, Xuejun Chang, Yue Chang, Zhe Chang, Xinyuan Chao, Wei Chatrabhuti, Auttakit Che, Yimin Che, Yuzhi Chen, Bin Chen, Danping Chen, Fuqing Chen, Fusan Chen, Gang Chen, Guoming Chen, Hua-Xing Chen, Huirun Chen, Jinhui Chen, Ji-Yuan Chen, Kai Chen, Mali Chen, Mingjun Chen, Mingshui Chen, Ning Chen, Shanhong Chen, Shanzhen Chen, Shao-Long Chen, Shaomin Chen, Shiqiang Chen, Tianlu Chen, Wei Chen, Xiang Chen, Xiaoyu Chen, Xin Chen, Xun Chen, Xurong Chen, Ye Chen, Ying Chen, Yukai Chen, Zelin Chen, Zilin Chen, Boping Chen, Chunhui Cheng, Hok Chuen Cheng, Huajie Cheng, Shan Cheng, Tongguang Chi, Yunlong Chimenti, Pietro Chiu, Wen Han Cho, Guk Chu, Ming-Chung Chu, Xiaotong Chu, Ziliang Coloretti, Guglielmo Crivellin, Andreas Cui, Hanhua Cui, Xiaohao Cui, Zhaoyuan D'Anzi, Brunella Dai, Ling-Yun Dai, Xinchen Dai, xuwen De Maria, Antonio De Filippis, Nicola De La Taille, Christophe De Mori, Francesca De Sio, Chiara Del Core, Elisa Deng, S Agh University of Science and Technology Faculty of Physics and Applied Computer Science Krakow Poland Anhui University Anhui Hefei China Applied Physics Department Federal Urdu University of Arts Science and Technology Islamabad Pakistan Banaras Hindu University Institute of Science Varanasi India Bandirma Onyedi Eylul University Bandirma Turkey Beihang University Beijing China Beijing Computational Science Research Center Beijing China Beijing Conveyi Ltd Beijing China Beijing Glass Research Institute Co. Ltd Beijing China Beijing Institute of Technology Beijing China Beijing Normal University Beijing China Brown University ProvidenceRI United States Budker Institute of Nuclear Physics Novosibirsk Russia Cadi Ayyad University Laboratory of Fundamental and Applied Physics Polydisciplinary Faculty Marrakech Morocco Cairo University Giza Egypt Center for Theory and Computation National Tsing Hua University Hsinchu Taiwan Central China Normal University Hubei Wuhan China Central South University Hunan Changsha China Centro de Fisica Teorica de Particulas Universidade Tecnica de Lisboa Lisboa Portugal Chengdu KaitengSifang Digital Radio & Tv Equipment Co. Ltd Sichuan Chengdu China China Academy of Engineering Physics Sichuan Mianyang China China Building Materials Academy Beijing China China Center of Advanced Science and Technology Beijing China China Institute of Atomic Energy Beijing China China Jiliang University Zhejiang Hangzhou China China Nuclear Instrument CO. Ltd Beijing China China University of Geosciences Beijing China China University of Geosciences Hubei Wuhan China China University of Mining and Technology Jiangsu Xuzhou China China West Normal University Sichuan Nanchong China Chongqing University Department of Physics Chongqing China Chulalongkorn University Bangkok Thailand City University of Hong Kong Department of Physics Hong Kong Cockcroft Institute Daresbury United Kingdom Cornell University IthacaNY

The Circular Electron Positron Collider (CEPC) is a large scientific projectinitiated and hosted by China, fostered through extensive collaboration withinternational partners. The complex comprises four accelerators: a 30 GeVLinac, a 1.1 GeV Damping Ring, a Booster capable of achieving energies up to180 GeV, and a Collider operating at varying energy modes (Z, W, H, and ttbar).The Linac and Damping Ring are situated on the surface, while the Booster andCollider are housed in a 100 km circumference underground tunnel, strategicallyaccommodating future expansion with provisions for a Super Proton ProtonCollider (SPPC). The CEPC primarily serves as a Higgs factory. In its baselinedesign with synchrotron radiation (SR) power of 30 MW per beam, it can achievea luminosity of 5e34 /cm^2/s^1, resulting in an integrated luminosity of 13 /abfor two interaction points over a decade, producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC\'s capability togenerate 4.3 million Higgs bosons, facilitating precise measurements of Higgscoupling at sub-percent levels, exceeding the precision expected from theHL-LHC by an order of magnitude. This Technical Design Report (TDR) follows thePreliminary Conceptual Design Report (Pre-CDR, 2015) and the Conceptual DesignReport (CDR, 2018), comprehensively detailing the machine\'s layout andperformance, physical design and analysis, technical systems design, R&D andprototyping efforts, and associated civil engineering aspects. Additionally, itincludes a cost estimate and a preliminary construction timeline, establishinga framework for forthcoming engineering design phase and site selectionprocedures. Construction is anticipated to begin around 2027-2028, pendinggovernment approval, with an estimated duration of 8 years. The commencement ofexperiments could potentially initiate in the mid-2030s. © 2023, CC BY-NC-SA.

关键词： Synchrotron radiation

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热应激对银鲫生化指标和HSP70 mRNA表达的影响

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渔业科学进展 2018年第6期39卷 65-71页

作者：周朝伟胡续雯雷骆刘晓庆任胜杰杨旻珉曹高祥陈国梁邓星星李岩李路宽西南大学动物科学学院水产系重庆402460

本研究旨在探讨热应激对银鲫(Carassius auratus gibelio)生化指标及肝脏HSP70 mRNA表达的影响,选取平均体重为(72.8±10.1) g的银鲫,分别在热应激前(25℃,对照组)、热应激后(32℃,0 h、2 h、6 h、10 h)和恢复适温后6 h (25℃,恢复... 详细信息

本研究旨在探讨热应激对银鲫(Carassius auratus gibelio)生化指标及肝脏HSP70 mRNA表达的影响,选取平均体重为(72.8±10.1) g的银鲫,分别在热应激前(25℃,对照组)、热应激后(32℃,0 h、2 h、6 h、10 h)和恢复适温后6 h (25℃,恢复组)的条件下,测定机体血浆乳酸(LD)、血浆葡萄糖(Glu)、肝糖原(Gly)、己糖激酶(HK)、丙酮酸激酶(PK)和肝脏HSP70 m RNA表达量变化。研究表明,实验组乳酸含量从热应激开始至恢复期,差异始终不显著(P>0.05);实验组血浆葡萄糖水平在热应激后0 h、2 h、6 h,极显著高于对照组(P<0.01);实验组肝糖原水平在热应激0 h时,极显著高于对照组(P<0.01),其余时间点差异不显著(P>0.05);己糖激酶活力在热应激后2 h,显著高于对照组(P>0.05);丙酮酸激酶活力在热应激后6h,极显著高于对照组(P<0.01);热应激后2h,肝脏中HSP70 m RNA表达量达到最高(P<0.01),恢复至适温后,表达量与应激前无差异(P>0.05)。本研究为进一步探究银鲫的高温应激作用机制及调控机理提供参考数据,同时,也为银鲫养殖和育种等生产实践提供相应参考依据。

关键词：银鲫热应激生化指标 HSP70

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Molecular characterization and expression profile of the estrogen receptor α gene during different reproductive phases in Monopterus albus

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SCIENTIFIC REPORTS 2016年第1期6卷 27924-27924页

作者： Ding, Weidong cao, Liping cao, Zheming Bing, xuwen Zhao, Fazhen Nanjing Agr Univ Wuxi Fisheries Coll 9 East Shanshui Rd Wuxi 214081 Peoples R China Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Minist Agr Key Lab Freshwater Fisheries & Germplasm Resource Wuxi 214081 Peoples R China Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Qingdao 266071 Peoples R China

To understand the molecular mechanism of estrogen and to evaluate the role of the estrogen receptor in mediating estrogen action, the full-length cDNA of estrogen receptor alpha (ER alpha) was cloned from Monopterus albus, and its expression pattern and distribution were investigated. The ERa cDNA of M. albus includes an open reading frame of 1863 bp, a 140-bp 5'-untranslated region and a 797-bp 3'-untranslated region. Amino acid sequence homology analysis showed that the Monopterus albus ER alpha has a moderate degree of similarity with Sebastes schlegelii, Zoarces viviparus and Haplochromis burtoni (81.1%, 80.7% and 80.4%, respectively). Quantitative PCR results showed that the highest level of ER alpha expression was in the liver;the next highest level of expression was observed in the gonads, where it was expressed at high levels particularly in the ovary in developmental stages IV and V and in the testis in developmental stage II/III. Immunohistochemistry analysis showed that ER alpha was present as slender particles distributed mainly in the membranes of spermatocytes and oocytes in the testis and ovary, whereas no positive signal was observed in the cytoplasm of sperm cells. This report describes the first molecular characterization of full-length ER alpha and its tissue-specific distribution in M. albus.

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建鲤基因组中一个ty3-gypsy反转录转座子的发现与分析

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中国水产科学 2016年第2期23卷 284-296页

作者：丁炜东曹丽萍曹哲明邴旭文农业部淡水渔业和种质资源利用重点实验室中国水产科学研究院淡水渔业研究中心江苏无锡214081

转座子是动植物基因组的重要组成部分,在前期研究中发现建鲤(Cyprinus carpio ***)基因组中存在一个ty3-gypsy反转录转座子类型的转座子,并将其命名为JRE转座子(Jian carp Retrotransposon,JRE)。为了研究JRE反转录转座子在建鲤基因组... 详细信息

转座子是动植物基因组的重要组成部分,在前期研究中发现建鲤(Cyprinus carpio ***)基因组中存在一个ty3-gypsy反转录转座子类型的转座子,并将其命名为JRE转座子(Jian carp Retrotransposon,JRE)。为了研究JRE反转录转座子在建鲤基因组中的功能,采用PCR扩增、荧光定量PCR和原位杂交等方法对JRE转座子的特性进行了研究。JRE反转录转座子全长5126 bp,具有5?端470 bp和3?端453 bp长末端重复片段(long terminal repeat end,LTR),中间的开放阅读框(ORF)包括核心蛋白基因(gag)和酶基因区域(pol),其长度为4203 bp。pol基因具有典型的ty3-gypsy反转录转座子结构,基因顺序为PR-RT-RH-IN基因。对pol基因的同源分析表明,其与虾夷扇贝(Mizuhopecten yessoensis)、栉孔扇贝(Azumapecten farreri)、大堡礁海绵(Xiphophorus maculates)和斑剑尾鱼(Xiphophorus maculates)pol基因相似性分别为40.7%、40.0%、32.8%和30.1%,因此JRE可能属于JULE反转录转座子家族。采用实时定量PCR对JRE转座子在建鲤基因组内的拷贝数进行了测定,结果表明其拷贝数为124,同时对不同组织中的m RNA表达量的研究表明,JRE转座子在建鲤肝、肾、血、肌肉、性腺5种组织中均有表达,在肾和肝中表达量略高。染色体原位杂交结果表明,JRE转座子在建鲤的染色体上随机分布,没有明显的规律性。本研究表明,JRE转座子具有典型的反转录转座子结构,属于JULE转座子的分枝,在染色体上的分布不多,其转录活性并不是很高,对我们了解建鲤基因组构成和特点增加了知识储备,同时为利用转座子的活性进行转基因研究提供了一种新的途径和工具。

关键词：建鲤反转录转座子拷贝数原位杂交

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