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中国动物传染病学报 2023年第3期31卷 74-81页

作者：侯立婷张媛媛于晓明杜露平张元鹏程海卫乔绪稳李丁郑其升陈瑾江苏省农业科学院动物免疫工程研究所南京210014 江苏省农业科学院国家兽用生物制品工程技术研究中心南京210014 兽用生物制品(泰州)国泰技术创新中心泰州225300 省部共建国家重点实验室培育基地—江苏省食品质量安全重点实验室南京210014

本研究将葡萄球菌A蛋白人工合成类似物的二聚体(DD)与口蹄疫的GHloop串联,按照大肠杆菌偏好性密码子优化合成GHloop-DD,并评价其免疫原性。将GHloop-DD插入到原核表达载体pET-32a中,鉴定阳性后转入*** BL21进行诱导表达。利用SDS-PAGE与... 详细信息

本研究将葡萄球菌A蛋白人工合成类似物的二聚体(DD)与口蹄疫的GHloop串联,按照大肠杆菌偏好性密码子优化合成GHloop-DD,并评价其免疫原性。将GHloop-DD插入到原核表达载体pET-32a中,鉴定阳性后转入*** BL21进行诱导表达。利用SDS-PAGE与Western blot对目的基因表达产物的可溶性进行分析。利用镍亲和层析柱纯化目的蛋白;利用BCA试剂盒测定重组蛋白的浓度,将重组蛋白(2.5μg/只)与206佐剂乳化,制备疫苗,免疫5周龄小鼠,同时设商品化合成肽苗对照与空白对照。免疫后28 d采集试验小鼠血清,利用口蹄疫病毒多肽ELISA抗体检测试剂盒检测免疫小鼠血清的抗体水平;利用流式细胞仪测定淋巴结B细胞的数量及骨髓中长寿浆细胞的数量;利用荧光定量检测相关趋化因子的表达水平;利用细胞因子检测试剂盒检测脾脏淋巴细胞刺激后上清液中的细胞因子的水平。SDS-PAGE结果表明,GHloop-DD基因在大肠杆菌中以可溶形式获得高水平表达;Western blot结果显示,表达的重组蛋白能够与特异性抗体发生反应。免疫试验结果表明,GHloop-DD不但能够刺激免疫小鼠产生高水平的多肽特异性ELISA抗体,而且能够提高淋巴结B细胞的数目及相关趋化因子水平,使骨髓中长寿浆细胞的数量和脾脏淋巴细胞刺激后上清液中细胞因子的水平显著提高。以上结果表明,本研究制备的GHloop-DD具有良好的免疫原性,为口蹄疫多肽疫苗的研制提供了新的方向。

关键词： GHloop-DD 体液免疫 B细胞长寿浆细胞

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Optimal Clearing Model of Centralized Market Considering Path Constraints

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IOP Conference Series: Earth and Environmental Science 2021年第1期657卷

作者： Zhao Zheng Kening chen Yu Jiang xuwen Liu State Grid Energy Research Institute Beijing China State Grid JiangSu Electric Power Co. Ltd Jiangsu China

China's electricity market is now still in the mode of 'Unified market and two-tier operation' including provincial and inter-provincial market. The inter-provincial market is divided into the medium and long-term market and spot market. This study takes the inter provincial spot market as the research object, and proposes a unified optimization clearing algorithm for inter provincial spot market considering path constraints. The proposed method is able to handle AC-DC hybrid electricity network, and sort and determine the trading pairs using power flow tracing algorithm. The power market software PLEXOS and practical market data are used to simulate the proposed model, and the results show the accuracy and effectiveness.

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全装配式钢-混凝土混合框架结构体系生产与施工技术研究

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上海建设科技 2023年第5期 1-4页

作者：亓立刚马明磊陈越时张士前常红城刘笑天肖绪文中国建筑第八工程局有限公司上海200122 中国工程院

针对中建八局自主研发的全装配式钢-混凝土混合框架结构,进行了相关生产和施工技术研究。为提高预制构件精度和现场施工效率,研究团队开发了一系列工艺流程、生产模具、施工工装和智能装备。预制节点和整体式预制柱分别采用立式和横式... 详细信息

针对中建八局自主研发的全装配式钢-混凝土混合框架结构,进行了相关生产和施工技术研究。为提高预制构件精度和现场施工效率,研究团队开发了一系列工艺流程、生产模具、施工工装和智能装备。预制节点和整体式预制柱分别采用立式和横式生产方式,保证了构件质量。在施工过程中,采用智能调姿技术、高效套筒灌浆技术、精准定位技术和免外架施工技术,实现了高精度、高效施工。临港中建总部基地项目的成功应用,证明了该结构体系在提高施工效率、降低工期和现场作业量方面的优势。该体系有望在建筑行业中得到更广泛应用,为实现绿色建筑和可持续发展作出更大贡献。

关键词：装配式框架结构预制柱-钢梁混合框架生产技术施工技术精准定位

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Reliability of Docking-Based Virtual Screening for GPCR Ligands with Homology Modeled Structures: A Case Study of the Angiotensin II Type I Receptor

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ACS CHEMICAL NEUROSCIENCE 2019年第1期10卷 677-689页

作者： chen, Haiyi Fu, Weitao Wang, Zhe Wang, xuwen Lei, Tailong Zhu, Feng Li, Dan Chang, Shan Xu, Lei Hou, Tingjun Zhejiang Univ Coll Pharmaceut Sci Hangzhou 310058 Zhejiang Peoples R China Zhejiang Univ State Key Lab CAD&CG Hangzhou 310058 Zhejiang Peoples R China Jiangsu Univ Technol Sch Elect & Informat Engn Inst Bioinformat & Med Engn Changzhou 213001 Peoples R China

The number of solved G-protein-coupled receptor (GPCR) crystal structures has expanded rapidly, but most GPCR structures remain unsolved. Therefore, computational techniques, such as homology modeling, have been widely used to produce the theoretical structures of various GPCRs for structure-based drug design (SBDD). Due to the low sequence similarity shared by the transmembrane domains of GPCRs, accurate prediction of GPCR structures by homology modeling is quite challenging. In this study, angiotensin II type I receptor (AT1R) was taken as a typical case to assess the reliability of class A GPCR homology models for SBDD. Four homology models of angiotensin II type I receptor (AT1R) at the inactive state were built based on the crystal structures of CXCR4 chemokine receptor, CCRS chemo-kine receptor, and delta-opioid receptor, and refined through molecular dynamics (MD) simulations and induced-fit docking, to allow for backbone and side-chain flexibility. Then, the quality of the homology models was assessed relative to the crystal structures in terms of two criteria commonly used in SBDD: prediction accuracy of ligand-binding poses and screening power of docking-based virtual screening. It was found that the crystal structures outperformed the homology models prior to any refinement in both assessments. MD simulations could generally improve the docking results for both the crystal structures and homology models. Moreover, the optimized homology model refined by MD simulations and induced-fit docking even shows a similar performance of the docking assessment to the crystal structures. Our results indicate that it is possible to establish a reliable class A GPCR homology model for SBDD through the refinement by integrating multiple molecular modeling techniques.

关键词： Docking-based virtual screening homology model class A GPCRs antagonist MD simulation induced-fit docking

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不同化学打顶剂对新疆棉花群体质量的影响

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中国棉花 2021年第10期48卷 21-24页

作者：王刚韩焕勇王旭文陈兵新疆农垦科学院新疆石河子832000

2020年以新陆早74号为研究对象,以人工打顶为对照,研究了甲哌钅翁和氟节胺不同剂量处理对棉花群体质量指标的影响,为棉花化学打顶技术的推广提供科学依据。结果表明:使用2种化学打顶剂后棉花叶面积指数、群体光吸收率、群体光合速率都... 详细信息

2020年以新陆早74号为研究对象,以人工打顶为对照,研究了甲哌钅翁和氟节胺不同剂量处理对棉花群体质量指标的影响,为棉花化学打顶技术的推广提供科学依据。结果表明:使用2种化学打顶剂后棉花叶面积指数、群体光吸收率、群体光合速率都随剂量的增加而下降,中剂量处理干物质积累量和蕾铃分配比最佳,同时中剂量处理的株高、单株果枝数、皮棉产量均高于对照处理。本研究初步明确了2种化学打顶剂不同剂量处理对棉花群体质量指标的影响,并证明了化学打顶替代人工打顶的可行性。

关键词：化学打顶剂群体质量棉花新疆叶面积指数光吸收率光合速率干物质积累量

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基于Sentinel-2与随机森林算法的太湖水生植被分布监测

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环境监控与预警 2023年第6期15卷 42-49页

作者：张悦陈冰李旭文牛志春姜晟李继影崔嘉宇余悠然江苏省环境监测中心江苏南京210019 江苏省生态环境大数据有限公司江苏南京210019 南京信息工程大学江苏南京210044 江苏省苏州环境监测中心江苏苏州215004

基于2018—2021年太湖地区哨兵2号(Sentinel-2)卫星遥感影像和随机森林算法,结合植被敏感指数,开展太湖水生植被分布监测,综合分析了水生植被的时空变化特征,初步揭示了太湖水生植被的动态变化。结果表明,太湖水生植被分布面积有逐年上... 详细信息

基于2018—2021年太湖地区哨兵2号(Sentinel-2)卫星遥感影像和随机森林算法,结合植被敏感指数,开展太湖水生植被分布监测,综合分析了水生植被的时空变化特征,初步揭示了太湖水生植被的动态变化。结果表明,太湖水生植被分布面积有逐年上升的趋势,且年间变化具有明显的单峰型特征,表现为春季沉寂,夏季快速增长,秋季达到暴发期,冬季面积开始减退;太湖水生植被主要分布于东太湖、东部沿岸和贡湖,类型以沉水植被为主,其中浮叶植被主要分布于东太湖,挺水植被主要分布于东太湖沿岸的浅水区域;沉水植被主要分布于东部沿岸和贡湖;水生植被主要分布的东太湖水域,其水质总体优于太湖其他水域。

关键词：哨兵2号卫星随机森林模型水生植被水质太湖

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Mapping human tissues with highly multiplexed RNA in situ hybridization

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NATURE COMMUNICATIONS 2024年第1期15卷 2511页

作者： Kalhor, Kian chen, Chien-Ju Lee, Ho Suk Cai, Matthew Nafisi, Mahsa Que, Richard Palmer, Carter R. Yuan, Yixu Zhang, Yida Li, xuwen Song, Jinghui Knoten, Amanda Lake, Blue B. Gaut, Joseph P. Keene, C. Dirk Lein, Ed Kharchenko, Peter V. Chun, Jerold Jain, Sanjay Fan, Jian-Bing Zhang, Kun Univ Calif San Diego Dept Bioengn La Jolla CA 92093 USA Univ Calif San Diego Program Bioinformat & Syst Biol La Jolla CA 92093 USA Univ Calif San Diego Dept Elect Engn La Jolla CA USA Sanford Burnham Prebys Med Discovery Inst La Jolla CA USA Univ Calif San Diego Program Biomed Sci La Jolla CA USA Harvard Med Sch Dept Biomed Informat Boston MA USA Altos Labs San Diego CA 94022 USA Washington Univ Sch Med Dept Med St Louis MO USA Washington Univ Sch Med Dept Pathol & Immunol St Louis MO USA Univ Washington Dept Lab Med & Pathol Sch Med Seattle WA USA Allen Inst Brain Sci Seattle WA 98103 USA Illumina San Diego CA USA

In situ transcriptomic techniques promise a holistic view of tissue organization and cell-cell interactions. There has been a surge of multiplexed RNA in situ mapping techniques but their application to human tissues has been limited due to their large size, general lower tissue quality and high autofluorescence. Here we report DART-FISH, a padlock probe-based technology capable of profiling hundreds to thousands of genes in centimeter-sized human tissue sections. We introduce an omni-cell type cytoplasmic stain that substantially improves the segmentation of cell bodies. Our enzyme-free isothermal decoding procedure allows us to image 121 genes in large sections from the human neocortex in <10 h. We successfully recapitulated the cytoarchitecture of 20 neuronal and non-neuronal subclasses. We further performed in situ mapping of 300 genes on a diseased human kidney, profiled >20 healthy and pathological cell states, and identified diseased niches enriched in transcriptionally altered epithelial cells and myofibroblasts.

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基于HX710的车辆称重物联系统设计

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自动化应用 2022年第4期 154-157,162页

作者：陈德富周旭文吴华君邱宝象屈亮亮浙江工业大学信息工程学院浙江杭州310023 万向钱潮股份有限公司浙江杭州311215

目前车辆载荷监测主要采用分离式称重设备进行固定点位称重,这种方式往往存在着精度低、耗时长以及设备铺设困难等问题。针对上述所面临的问题,设计了一种基于HX710的车辆称重物联系统。系统以嵌入式系统、HX710模数转换芯片、NB-IoT模... 详细信息

目前车辆载荷监测主要采用分离式称重设备进行固定点位称重,这种方式往往存在着精度低、耗时长以及设备铺设困难等问题。针对上述所面临的问题,设计了一种基于HX710的车辆称重物联系统。系统以嵌入式系统、HX710模数转换芯片、NB-IoT模块为核心,采集由电阻应变片组成应变电桥的电信号,经过处理后,通过无线通信模块发送至阿里云物联网平台,从而实现远程情况下的车辆载荷实时监测。通过实验验证,该系统实现了预期效果,具有实时性、高精度、装配快捷等特点。

关键词：车载称重 HX710 物联网嵌入式系统 NB-IoT

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Alumina nanoparticle modified phenol-formaldehyde resin as a wood adhesive

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INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES 2018年 81卷 79-82页

作者： Zhang, Rong Jin, Xiaobei Wen, xuwen chen, Qi Qin, Daochun Int Ctr Bamboo & Rattan Wangjing Area 8 Futongdongdajie Beijing Peoples R China

Phenol-formaldehyde resin was synthesized and modified by alumina nanoparticles (ANPs). The crystal form of the ANPs was characterized by X-ray diffraction (XRD), and differential scanning calorimetry (DSC) was used for thermal analysis of the resin. In addition, the resin was applied in plywood manufacture, and the curing time as well as the bonding strength was measured. The results showed, with an appropriate doping amount of ANPs, both the curing rate and the bonding strength increased significantly. The modified resin showed extensive application prospects in the wood construction industry.

关键词： Phenol-formaldehyde alumina nanoparticles plywood bonding strength

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Serum metabolomics reveals the intervention mechanism and compatible regularity of Chaihu Shu Gan San on chronic unpredictable mild stress-induced depression rat model

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JOURNAL OF PHARMACY AND PHARMACOLOGY 2020年第8期72卷 1133-1143页

作者： Zhang, Hongye Huang, Huimin Song, Hui Chin, Bonnie Zheng, Hua Ruan, Junxiang Wu, Fang cheng, Bang Wu, Jinxia Liu, xuwen Liang, Yonghong Song, Fangming chen, Zhaoni Tang, Chaoling Lu, Shiyin Guo, Hongwei Zou, Zhongmei Su, Zhiheng Guangxi Med Univ Pharmaceut Coll Nanning 530021 Peoples R China Shenyang Pharmaceut Univ Dept Clin Pharm Shenyang Peoples R China Guangxi Med Univ Med Sci Res Ctr Nanning Peoples R China Guangxi Med Univ Chinese Minist Educ Key Lab Longev & Aging Related Dis Nanning Peoples R China Guangxi Med Univ Ctr Translat Med Nanning Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Inst Med Plant Dev Beijing 100193 Peoples R China

Objectives To provide a comprehensive study of the intervention mechanism and compatible regularity of Chaihu Shu Gan San (CSGS) in a chronic unpredictable mild stress (CUMS)-induced depression model. Methods Ethological study and ELISA assay were applied to measure the phenotypes of depression after CUMS stimulate and assess the antidepressant activity of fluoxetine, CSGS and its compatibilities. The serum metabolic profile changes were revealed by untargeted Q/TOF MS-based metabolomics followed by multivariate statistical analysis. Key findings CSGS exhibits an significant intervention effect on CUMS-induced depression. After the multivariate statistical analysis, 17 potential serum biomarkers were identified and 16 of them could be regulated by CSGS. The intervention of CSGS on CUMS-induced depression involved five key pathways. Moreover, each functional unit (monarch, minister, assistant and guide medicine) in CSGS regulates different metabolites and metabolic pathways to achieve different effects on antidepressant;however, their intervention efficacies are inferior to the holistic formula, which may be due to the synergism of bioactive ingredients in the seven herbs of CSGS. Conclusions CSGS produced an obvious antidepressant activity. The comprehensive and holistic metabolomics approach could be a powerful tool to study the intervention mechanism and the compatibility rule of traditional Chinese medicine.

关键词： antidepression Chaihu Shu Gan San chronic unpredictable mild stress-induced depression compatibility serum metabolomics ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry

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