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畜牧与兽医 2023年第9期55卷 42-46页

作者：张元鹏侯立婷杜露平于晓明李兰杨利张浩明王义伟乔绪稳程海卫秦竹陈瑾郑其升江苏省农业科学院动物免疫工程研究所江苏南京210014 江苏省农业科学院国家兽用生物制品工程技术研究中心江苏南京210014 江苏省动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009 江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地江苏南京210014 兽用生物制品(泰州)国泰技术创新中心江苏泰州1225300

有研究证实,霍乱毒素(CT)A1亚基与葡萄球菌G蛋白的D肽偶联构建的融合蛋白CTA1-DD具有良好的佐剂效果。为了获得重组CTA1-DD蛋白在枯草芽胞杆菌中的表达,将CTA1-DD基因连接到表达载体pHT43中,构建重组枯草杆菌表达载体pHT43-CTA1-DD。将... 详细信息

有研究证实,霍乱毒素(CT)A1亚基与葡萄球菌G蛋白的D肽偶联构建的融合蛋白CTA1-DD具有良好的佐剂效果。为了获得重组CTA1-DD蛋白在枯草芽胞杆菌中的表达,将CTA1-DD基因连接到表达载体pHT43中,构建重组枯草杆菌表达载体pHT43-CTA1-DD。将重组载体转化到枯草芽胞杆菌WB600中,用IPTG诱导重组菌表达,发酵液离心后取上清液,通过SDS-PAGE和Western blot方法分析蛋白表达情况。发酵上清液经Ni-IDA琼脂糖树脂纯化,与流感病毒HA抗原混合后共同滴鼻免疫小鼠后采集小鼠血清和支气管肺泡灌洗液检测IgG和IgA的抗体水平。结果显示:重组CTA1-DD蛋白在枯草芽胞杆菌中能够分泌表达,在发酵罐中发酵36 h情况下蛋白表达量最高,目的蛋白大小在42 kDa左右,蛋白产量最高能到700μg/mL,Western blot检测目的蛋白正确表达。重组蛋白经过Ni-IDA琼脂亲和层析纯化后与HA抗原混和滴鼻免疫小鼠,ELISA结果显示CTA1-DD蛋白组针对HA抗原能够诱导更高的血清IgG和黏膜IgA抗体水平,说明枯草芽胞杆菌表达的CTA1-DD蛋白具有佐剂活性。试验结果为进一步研究CTA1-DD蛋白在黏膜佐剂的大规模生产应用奠定了基础。

关键词： CTA1-DD蛋白枯草芽胞杆菌表达黏膜免疫

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Modeling Hydro-Dynamics in a Harbor Area in the Daishan Island, China

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WATER 2019年第2期11卷 192页

作者： Li, Yuting Song, Zhiyao Peng, Guoqiang Fang, xuwen Li, Ruijie chen, Peng Hong, Haoyuan Nanjing Normal Univ Key Lab Virtual Geog Environm Nanjing 210023 Jiangsu Peoples R China State Key Lab Cultivat Base Geog Environm Evolut Nanjing 210023 Jiangsu Peoples R China Jiangsu Ctr Collaborat Innovat Geog Informat Reso Nanjing 210023 Jiangsu Peoples R China Anhui Transport Survey & Design Inst Co Ltd Hefei 230000 Anhui Peoples R China Hohai Univ Key Lab Coastal Disaster & Def Minist Educ Nanjing 210098 Jiangsu Peoples R China Hohai Univ Coll Oceanog Nanjing 210098 Jiangsu Peoples R China

This study presents an incorporation and application of a two-dimensional, unstructured-grid hydrodynamic model with a suspended sediment transport module in Daishan, China. The model is verified with field measurement data from 2017: water level, flow velocities and suspended sediment concentration (SSC). In the application on the Daishan, the performance of the hydrodynamic model has been satisfactorily validated against observed variations of available measurement stations. Coupled with the hydrodynamic model, a sediment transport model has been developed and tested. The simulations agreed quantitatively with the observations. The validated model was applied to the construction of breakwaters and docks under a different plan. The model can calculate the flow field and siltation situation under different breakwater settings. After we have analyzed the impact of existing breakwater layout schemes and sediment transport, a reasonable plan will be selected. The results show that the sea area near the north of Yanwo Shan and Dongken Shan has a large flow velocity exceeding 2.0 m/s and the flow velocity within the isobath of 5 m is small, within 0.6 m/s. According to the sediment calculation, the dock project is feasible. However, the designed width of the fairway should be increased to ensure the navigation safety of the ship according to variation characteristics of cross flow velocity in channel.

关键词： suspended sediment concentration numerical simulation breakwater the dock project

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A Dopant Replacement-Driven Molten Salt Method toward the Synthesis of Sub-5-nm-Sized Ultrathin Nanowires

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SMALL 2020年第23期16卷 2001098-2001098页

作者： Mao, Yihua Yang, xuwen Gong, Wenbin Zhang, Jing Pan, Ting Sun, Hongzhao chen, Zhigang Wang, Zhen Zhu, Junfa Hu, Jun Cong, Shan Geng, Fengxia Zhao, Zhigang Chinese Acad Sci Key Lab Nanodevices & Applicat Suzhou Inst Nanotech & Nanobion Suzhou 215123 Peoples R China Xuzhou Univ Technol Sch Phys & Energy Xuzhou 221018 Jiangsu Peoples R China Univ Sci & Technol China Natl Synchrotron Radiat Lab Hefei 230029 Peoples R China Chinese Acad Sci Key Lab Multifunct Nanomat & Smart Syst Suzhou 215123 Peoples R China Soochow Univ Coll Energy Suzhou 215123 Peoples R China Chinese Acad Sci Suzhou Inst Nanotech & Nanobion Div Nanomat Nanchang 330200 Jiangxi Peoples R China

The high-temperature molten-salt method is an important inorganic synthetic route to a wide variety of morphological phenotypes. However, its utility is limited by the fact that it is typically incapable of producing ultrathin (<5 nm diameter) nanowires, which have a crucial role in novel nanotechnology applications. Herein, a rapid molten salt-based synthesis of sub-5-nm-sized nanowires of hexagonal tungsten oxide (h-WO3) that is critically dependent on a substantial proportion of molybdenum (Mo) dopant is described. This dopant-driven morphological transition in tungsten oxide (WO3) may be attributable to the collapse of layered structure, followed by nanocluster aggregation, coalescence, and recrystallization to form ultrathin nanowires. Interestingly, due to the structural properties of h-WO3, the thus-formed ultrathin nanowires are demonstrated to be excellent photocatalysts for the production of ammonia (NH3) from nitrogen (N-2) and water. The ultrathin nanowires exhibit a high photocatalytic NH3-production activity with a rate of 370 mu mol g(-1) h(-1) and an apparent quantum efficiency of 0.84% at 420 nm, which is more than twice that obtained from the best-performing Mo-doped W18O49 nanowire catalysts. It is envisaged that the dopant replacement-driven synthetic protocol will allow for rapid access to a series of ultrathin nanostructures with intriguing properties and increase potential applications.

关键词： dopant replacement hexagonal structures molten-salt synthesis tungsten oxide ultrathin nanostructures

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A Novel Flavone from the Leaves of Murraya paniculata

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CHEMISTRY OF NATURAL COMPOUNDS 2018年第6期54卷 1061-1063页

作者： Li, Peng Zhang, Yue Wang, Ziqi chen, Honghui Li, xuwen Jin, Yongri Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China

A phytochemical investigation of the leaves of Murraya paniculata (L.) Jack resulted in a novel compound, 3,5-dihydroxy-5,7,4-trimethoxyflavone, together with 13 known compounds. The structure of the novel compound wa... 详细信息

关键词： Murraya paniculata (L.) Jack leaves flavonoids

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Research on Virtual Power Plant's Participation in Power Market under the Background of Energy Internet in China

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IOP Conference Series: Earth and Environmental Science 2021年第1期621卷

作者： Yang Su chen Kening Wang Yang Jiang Yu Ding Yu Liu xuwen Ma Qiuyang State Grid Energy Research Institute Beijing 102209 China State Grid JiangSu Electric Power Co. Ltd. JiangSu 210008 China

Under the background of energy internet, a large influx of market entities participates in the interaction of 'source network and load storage' and becomes an important resource for system regulation through the electricity marketization. Among them, the virtual power plant involves several market participators such as the distributed generation, demand side adjustable resources, distributed energy storage. The research of the operation mechanism and market-oriented trading organization for the virtual power plant becomes a popular topic in recent years. This paper proposes a novel mechanism for virtual power plants to participate in the electricity trading market, including the trading categories and auxiliary service which can be provided by the virtual power plant.

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Electronic structure and quasiparticle state character in the ferromagnetic Kondo lattice CeNiSb2

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Physical Review B 2025年第24期111卷 245113-245113页

作者： Bo Wang xuwen He Wei Feng Dengpeng Yuan Yi Liu Xiangfei Yang Yulu He Qin Liu Qunqing Hao Jian Wu Yun Zhang Zhengtai Liu Mao Ye Jishan Liu Shiyong Tan Qiuyun chen Xinchun Lai Science and Technology on Surface Physics and Chemistry Laboratory Mianyang 621907 China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research Institute Chinese Academy of Sciences Shanghai 201210 China

In a heavy-fermion system, the competition between the Ruderman-Kittel-Kasuya-Yosida (RKKY) interaction and the Kondo effect determines the ground-state properties, including superconductivity, magnetic orderings, and non-Fermi-liquid states. However, the temperature-dependent development of this competition remains unclear, particularly within the framework of ferromagnetic ground states. Here, the electronic structure and heavy quasiparticle band properties are exhaustively studied in CeNiSb2, a prototypical ferromagnetic Kondo lattice, using high-resolution angle-resolved photoemission spectroscopy. Our measurements reveal the three-dimensional electronic structure and Fermi surface topologies in CeNiSb2. The discrepancy of the quasiparticle bands along the Γ-M direction indicates band-dependent hybridization between the f electrons and conduction electrons (c−f hybridization), which may arise from differences in the conduction-band properties. The intensity of the quasiparticle band increases monotonically with decreasing temperature and deviates from the Kondo-like behavior at low temperature. This evolution provides a comprehensive picture of how the RKKY interaction and the Kondo effect develop and influence the behavior of f electrons across the temperature range. Our experimental results offer a spectroscopic study of the quasiparticle band and its temperature-dependent evolution in CeNiSb2, which may be crucial for understanding the underlying physics in heavy fermion compounds.

关键词： Ultraviolet photoelectron spectroscopy

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虎杖对水质和微生物群落结构及功能的影响

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环境科学与技术 2020年第5期43卷 25-32页

作者：陈家长赵志祥施羽露史磊磊郑尧邴旭文中国水产科学研究院淡水渔业研究中心农业部长江下游渔业生态环境监测中心农业部水产品质量安全环境因子风险评估实验室(无锡)江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214081

为研究虎杖种植后池塘水质变化和水体微生物群落碳源利用能力,该研究于室外构建了吉富罗非鱼养殖池塘5%虎杖生态浮床,2017年6-9月持续测定水质,利用Biolog技术分析池塘水体微生物群落结构及功能特征。结果表明6-7月生长期间虎杖种植能... 详细信息

为研究虎杖种植后池塘水质变化和水体微生物群落碳源利用能力,该研究于室外构建了吉富罗非鱼养殖池塘5%虎杖生态浮床,2017年6-9月持续测定水质,利用Biolog技术分析池塘水体微生物群落结构及功能特征。结果表明6-7月生长期间虎杖种植能有效削减池塘水体中TN、TP、Chl.a浓度,在温度较高的8、9月虎杖种植塘TP显著降低(P<0.05);7-9月虎杖种植塘水体微生物AWCD值显著增加(P<0.05),多样性McIntosh和Richness(除9月)指数均显著提高(P<0.05),且水体微生物对聚合糖类、氨基酸类、醇类的代谢能力显著提高(P<0.05)。在吉富罗非鱼养殖池塘种植虎杖,可在改善水质的基础上提高水体微生物群落多样性指数及对碳源的利用能力。

关键词：罗非鱼虎杖浮床微生物群落多样性碳源利用

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Thallium contamination in farmlands and common vegetables in a pyrite mining city and potential health risks

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ENVIRONMENTAL POLLUTION 2019年 248卷 906-915页

作者： Liu, Juan Li, Nuo Zhang, Weilong Wei, Xudong Tsang, Daniel C. W. Sun, Yubing Luo, xuwen Bao, Zhi'an Zheng, Wentao Wang, Jin Xu, Guoliang Hou, Liping chen, Yongheng Feng, Yuexing Guangzhou Univ Key Lab Water Qual & Conservat Pearl River Delta Guangdong Prov Key Lab Radionuclides Pollut Contr Inst Environm Res Greater BaySch Environm Sci & Guangzhou 510006 Guangdong Peoples R China Guangzhou Univ Rural Nonpoint Source Pollut Comprehens Managemen Guangzhou 510006 Guangdong Peoples R China Hong Kong Polytech Univ Dept Civil & Environm Engn Hung Hom Kowloon Hong Kong Peoples R China North China Elect Power Univ Coll Environm Sci & Engn MOE Key Lab Resources & Environm Syst Optimizat Beijing 102206 Peoples R China Northwest Univ Dept Geol State Key Lab Continental Dynam Xian 710069 Peoples R China Guangzhou Univ Sch Life Sci Guangzhou 510655 Guangdong Peoples R China Univ Queensland Sch Earth & Environm Sci Brisbane Qld 4072 Australia

Thallium (Tl) is a trace metal of severe toxicity. Its health concerns via consumption of contaminated vegetables have often been overlooked or underestimated. This study was designed to gain insight into the actual level and distribution characteristics of Tl and metal (loid)s (Pb, Cd, Cr, Sb, Mn, Cu, Zn, Ni, and Co) in agricultural soils and common vegetables cultivated in different zones (upstream, midstream, and downstream) of a densely populated residential area in a typical mine city, which has been open-pit exploiting Tl-bearing pyrite minerals since 1960s. The results show that most of the agricultural soils exhibit contaminated levels of Tl, with Tl contents (upstream: 1.35-4.31 mg/kg, midstream: 2.43 -5.19 mg/kg, and downstream: 0.65-233 mg/kg) mostly exceeding the maximum permissible level (MPL) for agricultural land use (1 mg/kg). Sequential extraction procedure indicates that even Tl is predominantly retained in the residual fraction, significant levels of Tl are still present in the geochemically mobile fractions. Besides, metals like Cu, Cd, Mn, and Co are mostly distributed in the labile fractions. Almost all metal (loid)s in edible parts of the vegetables exceed their corresponding MPL for consumption. The chronic daily intake (CDI) and hazard quotient (HQ) values calculated for inhabitants at different ages indicate non-negligible Tl risks via consumption of local vegetables, especially for children. Therefore, it is critical to establish effective measures for hazardous waste management and enforceable regulations in Tl-polluted area to mitigate potential severe impacts of Tl on human health through food chain. (C) 2019 Elsevier Ltd. All rights reserved.

关键词： Thallium Agricultural soil Vegetable Metal/metalloid contamination Pyrite mining

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基于断路器保护的配电线路最大敷设长度研究

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福建建筑 2016年第1期 95-98页

作者：陈旭文福州市规划设计研究院福建福州350108

根据国家标准GB10963、GB50054、GB/T15544对断路器短路时电磁脱扣的要求,以及结合最小短路电流计算的方法,推导出满足断路器短路保护要求的不同截面配电线路的最大敷设长度计算公式,计算出短路时断路器对配电线路的最大保护长度数值。... 详细信息

根据国家标准GB10963、GB50054、GB/T15544对断路器短路时电磁脱扣的要求,以及结合最小短路电流计算的方法,推导出满足断路器短路保护要求的不同截面配电线路的最大敷设长度计算公式,计算出短路时断路器对配电线路的最大保护长度数值。计算结果表明,低压配电线路并不是在安装了短路保护断路器后就可以无限长敷设,当超过断路器最大保护长度时,断路器将不能可靠跳闸,线路将失去保护。

关键词：断路器配电线路短路保护最大长度瞬时脱扣

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单纯修补术与胃大部切除术治疗急性胃穿孔的效果分析

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中国社区医师 2016年第5期32卷 25-25,27页

作者：陈绪文湖北省武汉市蔡甸区大集街卫生院 430100

目的:探讨单纯修补术与胃大部切除术治疗急性胃穿孔的效果。方法:收治急性胃穿孔患者80例,随机分为对照组和试验组各40例。对照组采取胃大部切除术治疗,试验组采取单纯修补术治疗,比较两组的治疗效果。结果:试验组的住院时间、胃肠道功... 详细信息

目的:探讨单纯修补术与胃大部切除术治疗急性胃穿孔的效果。方法:收治急性胃穿孔患者80例,随机分为对照组和试验组各40例。对照组采取胃大部切除术治疗,试验组采取单纯修补术治疗,比较两组的治疗效果。结果:试验组的住院时间、胃肠道功能恢复时间以及总有效率明显优于对照组(P<0.05)。结论:对急性胃穿孔患者采取单纯修补术进行治疗,能够有效缩短患者的住院时间和胃肠道功能恢复时间,治疗效果显著。

关键词：单纯修补术胃大部分切除术急性胃穿孔

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