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Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus

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SPRINGERPLUS 2016年第1期5卷 338页

作者： Zheng, Yao Qu, Jianhong Qiu, Liping Fan, Limin Meng, Shunlong Song, Chao Bing, xuwen chen, Jiazhang Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr 9 Shanshui East Rd Wuxi 214081 Jiangsu Peoples R China Sci Observing & Expt Stn Fishery Resources & Envi Wuxi 214081 Peoples R China Nanjing Agr Univ Wuxi Fisheries Coll Wuxi 214081 Peoples R China

The normal dose of 17 alpha-methyltestosterone (MT) used in fish farming was 60 mg/L, and now the analysis of residual androgens was carried out in waste water obtained from the Beijing area, which could be detected in levels ranging from 4.1 to 7.0 ng/L. For the purpose of aquatic early warning, the present study clearly demonstrated that chronic exposure by higher concentration of MT than environmental relevant concentrations could trigger oxidative stress response to juvenile tilapia by modulating hepatic antioxidant enzyme activities and gene transcription. Some antioxidative parameters (T-GSH, GSH/GSSG and MDA) were significant decreased under 0.5 mg/L MT exposure at 7 and 14 days. Some antioxidant enzymes (SOD, CAT and GST) and transcriptional changes (sod and cat) were revealed significant decreases for MT treated groups at 7 days. Total antioxidant capacity was significant increased only in 5 mg/L MT exposure groups, but GR activities were not affected all through the whole exposure period. Almost all of the antioxidant enzymatic genes detected in the present study were showed significant increments for MT exposure both at 14 and 21 days, and the genotoxicity profile of antioxidant enzymatic genes were revealed dose-dependent manner. This study presented evidence that MT could result in oxidative stress response in the early stages of GIFT tilapia.

关键词： 17 alpha-Methyltestosterone Antioxidant enzyme Genotoxicity Oreochromis niloticus Oxidative stress

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氧化还原修复重金属污染土壤的文献计量学分析

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环境保护科学 2017年第2期43卷 116-123页

作者：马妍陈家琪徐东耀左海清门铭何绪文中国矿业大学(北京)化学与环境工程学院北京100083

基于Web of Science和中国知网(CNKI)的数据源,选择对应数据库、设定年限,检索相关文献和专利,运用文献计量学方法,并借助Origin、Excel等绘图制表工具对数据结果进行了统计、分析和对比,比较直观地展现出重金属污染土壤氧化还原修复技... 详细信息

基于Web of Science和中国知网(CNKI)的数据源,选择对应数据库、设定年限,检索相关文献和专利,运用文献计量学方法,并借助Origin、Excel等绘图制表工具对数据结果进行了统计、分析和对比,比较直观地展现出重金属污染土壤氧化还原修复技术的总体情况、国内外发展现状和趋势、国内外发展差距,并从关键词的发展演替角度分析研究热点与方向,为氧化还原修复技术相关学科发展与研究等提供决策参考。

关键词：氧化还原重金属土壤修复文献计量学

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Interdiffusivities and atomic mobilities in FCC Co-Mo-W alloys

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CALPHAD-COMPUTER COUPLING OF PHASE DIAGRAMS AND THERMOCHEMISTRY 2015年 49卷 35-40页

作者： He, xuwen Zhang, Weibin Yan, Mingyuan chen, Chong Du, Yong Zhang, Lijun Huang, Bai-yun Cent S Univ State Key Lab Powder Met Changsha 410083 Hunan Peoples R China

By employing six groups of bulk diffusion couples together with electron probe microanalysis technique, the composition dependence of ternary interdiffusion coefficients in Co-rich fcc Co-Mo-W alloys at 1373 K was determined via the Whittle and Green method. The experimental interdiffusion coefficients were critically assessed to obtain the atomic mobilities of Co, Mo and Win fcc Co-Mo-W alloys by using the DICTRA (Diffusion-Controlled TRAnsformations) software package. Comprehensive comparisons between the calculated and experimental data show that a good agreement is obtained for diffusivities in binary Co-Mo and ternary Co-Mo-W systems. In addition, a further verification of the obtained atomic mobilities was carried out through comparing the model-predicted concentration profiles/diffusion paths of several diffusion couples with the corresponding experimental data. The results indicate that the atomic mobilities can reproduce the experiment data reasonably well. This work contributes to the establishment of a Co-based kinetic database for computational design of superalloys. (C) 2015 Elsevier Ltd. All rights reserved.

关键词： Diffusion couple Atomic mobility Fcc Co-Mo-W alloys DICTRA Fick's law

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水培大蒜技术在水产养殖中应用可能性初步研究

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中国农学通报 2017年第32期33卷 155-158页

作者：范立民邴旭文裘丽萍孟顺龙宋超陈家长中国水产科学研究院淡水渔业研究中心/农业部长江下游渔业生态环境监测中心/中国水产科学研究院内陆生态环境和资源重点开放实验室江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214081

旨在研究水培大蒜在水产养殖中应用的基础性问题。通过在红斑马鱼(Brachydanio rerio)养殖缸中浮床种养大蒜(Allium sativum L.)试验,研究水培大蒜对红斑马鱼自然死亡率的影响试验;通过在异育银鲫(Carassius auratus gibelio)养殖缸中... 详细信息

旨在研究水培大蒜在水产养殖中应用的基础性问题。通过在红斑马鱼(Brachydanio rerio)养殖缸中浮床种养大蒜(Allium sativum L.)试验,研究水培大蒜对红斑马鱼自然死亡率的影响试验;通过在异育银鲫(Carassius auratus gibelio)养殖缸中浮床种植大蒜试验,研究水培大蒜对养殖水体水质的影响。结果表明:当大蒜种植量达到4.75 g/L时斑马鱼的自然死亡率显著增加,这可能与溶解氧的降低有关;大蒜种植使得水体总磷、总氮、氨氮、高锰酸盐指数和亚硝态氮水平均发生显著下降,其中对氨氮的影响最显著。水培大蒜可以改善养殖水体水质,但要注意控制种养密度,水培大蒜在水产养殖中的应用具有一定的前景。

关键词：水培大蒜水产养殖水质净化

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BTK INHIBITOR

BTK INHIBITOR

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作者： WANG, Xuehai WU, chengde XU, Yong SHEN, Chunli LI, Li'e HU, Guoping YUE, Yang LI, Jian GUO, Diliang SHI, Nengyang HUANG, Lu chen, Shuhui TU, Ronghua YANG, Zhongwen ZHANG, xuwen XIAO, Qiang TIAN, Hua YU, Yanping chen, Hailiang SUN, Wenjie HE, Zhenyu SHEN, Jie YANG, Jing TANG, Jing ZHOU, Wen YU, Jing ZHANG, Yi LIU, Quan

Provided are a series of BTK inhibitors, and specifically disclosed are a compound, pharmaceutically acceptable salt thereof, tautomer thereof or prodrug thereof represented by formula (I), (II), (III) or (IV).

标准号: EP3246317(A4)

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二溴海因对吉富罗非鱼血浆抗氧化酶基因表达的影响

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环境科学与技术 2017年第2期40卷 39-43页

作者：郑尧邴旭文裘丽萍范立民宋超孟顺龙刘涛孙博李丹丹张聪陈家长中国水产科学研究院淡水渔业研究中心农业部淡水鱼类遗传育种与养殖生物学重点开放实验室农业部长江下游渔业资源环境科学观测实验站中国水产科学研究院内陆渔业生态环境和资源重点开放实验室江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214081

研究不同时间点(1、2、4、8、16 d)及修复期(26 d)、不同浓度(0、0.06、0.3、1.5 mg/L)二溴海因(DBDMH)对雄性吉富罗非鱼血浆中抗氧化酶基因的基因毒性,筛选稳定的生物标志物。实时荧光定量PCR方法检测DBDMH对超氧化物歧化酶(sod)、谷... 详细信息

研究不同时间点(1、2、4、8、16 d)及修复期(26 d)、不同浓度(0、0.06、0.3、1.5 mg/L)二溴海因(DBDMH)对雄性吉富罗非鱼血浆中抗氧化酶基因的基因毒性,筛选稳定的生物标志物。实时荧光定量PCR方法检测DBDMH对超氧化物歧化酶(sod)、谷胱甘肽-S-转移酶(gst)、谷胱甘肽还原酶(gr)、谷胱甘肽过氧化物酶1(gpx1)、过氧化氢酶(cat)基因表达的影响。中浓度组雄性吉富罗非鱼血浆抗氧化酶基因(除gr)表达受DBDMH胁迫诱导显著上调;各DBDMH浓度组1 d(sod,gst,gpx1),2 d(gst,gr)抗氧化基因表达呈现浓度依赖性下降,4 d sod,gpx1基因表达呈现浓度依赖性上升;8 d中浓度DBDMH处理组基因表达(gst,gr,gpx1)显著低于低浓度组;恢复试验阶段,中、高浓度DBDMH组各抗氧化酶基因表达均不能恢复到正常水平。0.3 mg/L DBDMH显著增强雄性吉富罗非鱼血浆所测抗氧化酶基因的表达(除1 d gpx1、cat,8和16 d gr),此浓度下抗氧化酶基因作为标志物较合适。

关键词：吉富罗非鱼二溴海因血浆抗氧化酶基因

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Structural Determinants for the Strict Monomethylation Activity by Trypanosoma brucei Protein Arginine Methyltransferase 7

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STRUCTURE 2014年第5期22卷 756-768页

作者： Wang, Chongyuan Zhu, Yuwei Caceres, Tamar B. Liu, Lei Peng, Junhui Wang, Junchen chen, Jiajing chen, xuwen Zhang, Zhiyong Zuo, Xiaobing Gong, Qingguo Teng, Maikun Hevel, Joan M. Wu, Jihui Shi, Yunyu Univ Sci & Technol China Hefei Natl Lab Phys Sci Microscale & Sch Life Sci Hefei 230027 Anhui Peoples R China Utah State Univ Dept Chem & Biochem Logan UT 84322 USA Argonne Natl Lab Adv Photon Source Chicago IL 60437 USA

Trypanosoma brucei protein arginine methyltransferase 7 (TbPRMT7) exclusively generates monomethylarginine (MMA), which directs biological consequences distinct from that of symmetric dimethylarginine (SDMA) and asymmetric dimethylarginine (ADMA). However, determinants controlling the strict monomethylation activity are unknown. We present the crystal structure of the TbPRMT7 active core in complex with S-adenosyl-L-homocysteine (AdoHcy) and a histone H4 peptide substrate. In the active site, residues E172, E181, and Q329 hydrogen bond the guanidino group of the target arginine and align the terminal guanidino nitrogen in a position suitable for nucleophilic attack on the methyl group of S-adenosyl-L-methionine (AdoMet). Structural comparisons and isothermal titration calorimetry data suggest that the TbPRMT7 active site is narrower than those of protein arginine dimethyltransferases, making it unsuitable to bind MMA in a manner that would support a second turnover, thus abolishing the production of SDMA and ADMA. Our results present the structural interpretations for the monomethylation activity of TbPRMT7.

关键词： PROTEIN arginine methyltransferases METHYLATION TRYPANOSOMA brucei ASYMMETRIC dimethylarginine CRYSTAL structure PEPTIDES HYDROGEN bonding

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HPLC梯度洗脱法测定丙泊酚中/长链脂肪乳注射液的含量及有关物质

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中国药品标准 2016年第5期17卷 347-352页

作者：焦旭雯梁蔚阳陈华广东省药品检验所广州510180

目的:建立测定丙泊酚中/长链脂肪乳注射液中的丙泊酚及其有关物质的HPLC方法。方法:色谱柱为Waters XBridge RP18柱(4.6 mm×150 mm,5μm);流动相为磷酸二氢钠溶液-乙腈,梯度洗脱;柱温为40℃;检测波长为275 nm和254 nm。丙泊酚的测... 详细信息

目的:建立测定丙泊酚中/长链脂肪乳注射液中的丙泊酚及其有关物质的HPLC方法。方法:色谱柱为Waters XBridge RP18柱(4.6 mm×150 mm,5μm);流动相为磷酸二氢钠溶液-乙腈,梯度洗脱;柱温为40℃;检测波长为275 nm和254 nm。丙泊酚的测定采用外标法,有关物质检查采用外标法与自身对照法。结果:丙泊酚与其两杂质能较好分离,相邻各峰间分离度均大于1.5;丙泊酚、杂质Ⅰ和杂质Ⅱ的线性范围分别为97.2~1 457.9μg·m L^(-1)(r=0.999 9)、0.11~1.57μg·m L^(-1)(r=0.999 9)和0.11~1.59μg·m L^(-1)(r=0.999 9);平均回收率(n=9)分别为99.2%(RSD=1.4%)、105.8%(RSD=1.5%)和96.7%(RSD=4.8%)。结论:所建方法简便快速、专属性强、重复性好,可用于丙泊酚中/长链脂肪乳注射液的含量测定和杂质检查。

关键词：高效液相色谱法丙泊酚有关物质含量测定

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用超声波测距的四旋翼无人机三维防撞系统设计

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单片机与嵌入式系统应用 2016年第10期16卷 40-43页

作者：陈根华黎嘉明葛旭文南昌工程学院信息工程学院南昌330099

针对四旋翼无人机碰撞事故多发的问题,设计了一款基于超声波测距原理的四旋翼无人机三维防撞系统。本系统利用超声波技术实现测距,将无人机与周围障碍间三维空间方向上的距离信息采集到控制系统,控制系统根据距离信息控制悬停信号与遥... 详细信息

针对四旋翼无人机碰撞事故多发的问题,设计了一款基于超声波测距原理的四旋翼无人机三维防撞系统。本系统利用超声波技术实现测距,将无人机与周围障碍间三维空间方向上的距离信息采集到控制系统,控制系统根据距离信息控制悬停信号与遥控信号,实现无人机自主防撞,最后通过试验证明了该系统的正确性与合理性。该系统满足了四旋翼无人机安全防撞要求,且具有重量轻、体积小、精度高、安全可靠等特点。

关键词：四旋翼无人机超声波测距三维防撞系统

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The wide utility of rabbits as models of human diseases

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EXPERIMENTAL AND MOLECULAR MEDICINE 2018年第5期50卷 66-66页

作者： Esteves, Pedro J. Abrantes, Joana Baldauf, Hanna-Mari BenMohamed, Lbachir chen, Yuxing Christensen, Neil Gonzalez-Gallego, Javier Giacani, Lorenzo Hu, Jiafen Kaplan, Gilla Keppler, Oliver T. Knight, Katherine L. Kong, Xiang-Peng Lanning, Dennis K. Le Pendu, Jacques de Matos, Ana Lemos Liu, Jia Liu, Shuying Lopes, Ana M. Lu, Shan Lukehart, Sheila Manabe, Yukari C. Neves, Fabiana McFadden, Grant Pan, Ruimin Peng, xuwen de Sousa-Pereira, Patricia Pinheiro, Ana Rahman, Masmudur Ruvoen-Clouet, Natalie Subbian, Selvakumar Tunon, Maria Jesus van der Loo, Wessel Vaine, Michael Via, Laura E. Wang, Shixia Mage, Rose Univ Porto CIBIO InBIO Res Network Biodivers & Evolutionary Biol Campus VairaoRua Padre Armando Quintas P-4485661 Vairao Portugal Univ Porto Dept Biol Fac Ciencias Rua Campo Alegre S-N P-4169007 Porto Portugal Inst Invest & Formacao Avancada Ciencias & Tecnol Gandra Portugal LMU Munchen Max von Pettenkofer Inst Fac Med Natl Reference Ctr Retroviruses D-81377 Munich Germany LMU Munchen Gene Ctr Fac Med VirolNatl Reference Ctr Retroviruses D-81377 Munich Germany Univ Calif Irvine Gavin Herbert Eye Inst Lab Cellular & Mol Immunol Sch Med Irvine CA 92697 USA Univ Calif Irvine Sch Med Dept Mol Biol & Biochem Irvine CA 92697 USA Univ Calif Irvine Inst Immunol Sch Med Sch Irvine Irvine CA 92697 USA Univ Massachusetts Med Sch Dept Med Worcester MA 01605 USA Penn State Univ Dept Pathol Hershey PA USA Penn State Univ Dept Microbiol & Immunol Hershey PA USA Penn State Univ Dept Comparat Med Hershey PA USA Univ Leon Inst Biomed IBIOMED Leon 24071 Spain Univ Leon CIBERehd Leon 24071 Spain Univ Washington Dept Med Seattle WA USA Univ Washington Dept Global Hlth Seattle WA 98195 USA Bill & Melinda Gates Fdn Seattle WA USA Loyola Univ Chicago Dept Microbiol & Immunol Maywood IL 60153 USA NYU Sch Med Dept Biochem & Mol Pharmacol New York NY 10016 USA Univ Nantes Univ Angers CRCINA INSERM Nantes France Arizona State Univ Biodesign Inst Ctr Immunotherapy Vaccines & Virotherapy Tempe AZ 85287 USA Univ Arkansas Med Sci Dept Microbiol & Immunol Little Rock AR 72205 USA Univ Porto Inst Biomed Sci Abel Salazar ICBAS Dept Anat Porto Portugal Univ Porto Inst Biomed Sci Abel Salazar ICBAS Unit Multidisciplinary Res Biomed UMIB Porto Portugal Johns Hopkins Univ Sch Med Dept Med Div Infect Dis Baltimore MD 21205 USA Rutgers State Univ PHRI New Jersey Med Sch RBHS Newark NJ USA NIAID Tubercolosis Res Sect Lab Clin Infect Dis Div Intramural ResNIH 9000 Rockville Pike Bethesda MD 20892 USA Univ Cape Town Dept Clin Lab Sci Inst Infect

Studies using the European rabbit Oryctolagus cuniculus contributed to elucidating numerous fundamental aspects of antibody structure and diversification mechanisms and continue to be valuable for the development and testing of therapeutic humanized polyclonal and monoclonal antibodies. Additionally, during the last two decades, the use of the European rabbit as an animal model has been increasingly extended to many human diseases. This review documents the continuing wide utility of the rabbit as a reliable disease model for development of therapeutics and vaccines and studies of the cellular and molecular mechanisms underlying many human diseases. Examples include syphilis, tuberculosis, HIV-AIDS, acute hepatic failure and diseases caused by noroviruses, ocular herpes, and papillomaviruses. The use of rabbits for vaccine development studies, which began with Louis Pasteur's rabies vaccine in 1881, continues today with targets that include the potentially blinding HSV-1 virus infection and HIV-AIDS. Additionally, two highly fatal viral diseases, rabbit hemorrhagic disease and myxomatosis, affect the European rabbit and provide unique models to understand co-evolution between a vertebrate host and viral pathogens.

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