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A FRET-based fluorescent probe for mercury ions in water and living cells

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2016年 165卷 99-105页

作者： Zhang, Bo Ma, Pinyi Gao, Dejiang Wang, Xinghua Sun, Ying song, Daqian Li, xuwen Jilin Univ Coll Chem Qianjin St 2699 Changchun 130012 Peoples R China Changchun Jilin Univ Little Swan Instruments Co Ltd Chuangxin Rd 1203 Changchun 130012 Peoples R China

On the basis of fluorescence resonance energy transfer (FRET), a new rhodamine derivative (DRh) was synthesized as a ratiometric fluorescent probe for detecting Hg2+ in water and living cells samples. The recognition properties of the probe DRh with metal ions had been investigated in H2O/CH3CN (9:1, v/v;Tris-HCl 50 mmol L-1;pH = 7.0) solution by the UV-Vis spectrophotometry and the fluorescence spectrophotometry. The results showed that the probe DRh exhibited the selective recognition of Hg2+. Upon the addition of Hg2+, the spirolactam ring of probe DRh was opened. The 1:1 stoichiometric structure between DRh and Hg2+ were supported by Job's plot, MS and OFT theoretical calculations. The linearly fluorescence intensity ratio (I-582/I-538) is proportional to the concentration of Hg2+ in the range 0-30 mu mol L-1. The limit of detection (LOD) of Hg2+ is 0.008 mu mol L-1 (base on S/N = 3). The present probe was applied to the determination of Hg2+ in neutral water samples and gave recoveries ranging from 104.5 to 107.9%. Furthermore, the fluorescent probe also can be applied as a bioimaging reagent for Hg2+ detection in HeLa cells. (C) 2016 Elsevier B.V. All rights reserved.

关键词： Mercury ions Fluorescence probe Rhodamine FRET Water Living cells DFT

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中国南海天然气水合物第二次试采主要进展

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中国地质 2020年第3期47卷 557-568页

作者：叶建良秦绪文谢文卫卢海龙马宝金邱海峻梁金强陆敬安匡增桂陆程梁前勇魏士鹏于彦江刘春生李彬申凯翔史浩贤卢秋平李晶寇贝贝宋刚李博张贺恩陆红锋马超董一飞边航中国地质调查局北京100037 中国地质调查局广州海洋地质调查局广东广州510075 中国地质调查局勘探技术研究所河北廊坊065000 北京大学北京100871 中国石油集团海洋工程有限公司北京100102 中国地质调查局油气调查中心北京100083 南方海洋科学与工程广东省实验室(广州) 广东广州510000

泥质粉砂型天然气水合物被认为是储量最大开采难度亦最大的水合物储层,2017年南海天然气水合物试采,初步验证了此类水合物储层具备可开采性。在总结前次试采认识的基础上,对试采矿体进行优选、精细评价、数值与试验模拟和陆地试验,中国... 详细信息

泥质粉砂型天然气水合物被认为是储量最大开采难度亦最大的水合物储层,2017年南海天然气水合物试采,初步验证了此类水合物储层具备可开采性。在总结前次试采认识的基础上,对试采矿体进行优选、精细评价、数值与试验模拟和陆地试验,中国地质调查局于2019年10月-2020年4月在南海水深1225 m神狐海域进行了第二次天然气水合物试采。本次试采攻克了钻井井口稳定性、水平井定向钻进、储层增产改造与防砂、精准降压等一系列深水浅软地层水平井技术难题,实现连续产气30 d,总产气量86.14×104m3,日均产气2.87×104m3,是首次试采日产气量的5.57倍,大大提高了日产气量和产气总量。试采监测结果表明,整个试采过程海底、海水及大气甲烷含量无异常。本次成功试采进一步表明,泥质粉砂储层天然气水合物具备可安全高效开采的可行性。

关键词：南海天然气水合物第二次试采水平井储层改造环境影响油气资源工程神狐海域南海

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浮床虎杖对不同浓度铵盐的降解效果研究

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中国农学通报 2019年第2期35卷 153-157页

作者：郑尧胡庚东裘丽萍吴伟孟顺龙范立民宋超邴旭文陈家长中国水产科学研究院淡水渔业研究中心农业部水产品质量安全环境因子风险评估实验室(无锡)江苏无锡214081

本研究旨在探究浮床虎杖对不同浓度氨氮的降解效果。通过室内大桶构建浮床虎杖,添加不同浓度组铵盐(0,1,5,10 mg/L)测定TN、NH_4^+-N、NO_3--N、NO_2--N、TP等水质指标。结果表明第5～13天4种不同浓度铵盐TN浓度下降,第17天升高;10 mg/... 详细信息

本研究旨在探究浮床虎杖对不同浓度氨氮的降解效果。通过室内大桶构建浮床虎杖,添加不同浓度组铵盐(0,1,5,10 mg/L)测定TN、NH_4^+-N、NO_3--N、NO_2--N、TP等水质指标。结果表明第5～13天4种不同浓度铵盐TN浓度下降,第17天升高;10 mg/L铵盐组NH_4^+-N浓度第5～13天下降;敞水区5和10 mg/L铵盐组NO_3--N浓度先升高后降低,根系区分别在第5天和第9天达到峰值;10 mg/L铵盐组NO_2--N浓度第5天升高至峰值第13天降至0;对照组TP浓度高于其他铵盐浓度组。浮床虎杖可在第9～13天内能造成TN、NH_4^+-N、NO_3--N、NO_2--N浓度下降,但TP浓度降至0需要更长时间。

关键词：水质虎杖铵盐降解

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“空心菜-水芹”轮作对养殖池塘水质和底质环境的影响

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上海海洋大学学报 2018年第1期27卷 98-105页

作者：郑尧陈家长胡庚东赵志祥孟顺龙范立民宋超邴旭文中国水产科学研究院淡水渔业研究中心江苏无锡214081 农业部水产品质量安全环境因子风险评估实验室江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214081

通过测定TOC、COD、Chl、TN、NH_4^+-N、NO_2^--N、NO_3^--N、TP、PO_4^(3-)-P等水质指标和底泥中TOC、TN、TP指标,探究"空心菜-水芹"轮作模式对不同养殖品种和养殖数量情况下养殖池塘水质和底质环境的影响。结果表明,在轮作... 详细信息

通过测定TOC、COD、Chl、TN、NH_4^+-N、NO_2^--N、NO_3^--N、TP、PO_4^(3-)-P等水质指标和底泥中TOC、TN、TP指标,探究"空心菜-水芹"轮作模式对不同养殖品种和养殖数量情况下养殖池塘水质和底质环境的影响。结果表明,在轮作模式前期,空心菜(Ipomoea aquatica)种植能显著降低甘露青鱼(Mylopharyngodon piceus)养殖场TOC、Chl、TN、NH_4^+-N、NO_2^--N、NO_3^--N、PO_4^(3-)-P等水质指标,能显著降低苏州经济鱼亲本塘TOC、COD、Chl、NH_4^+-N、NO_2^--N、NO_3^--N、TP等水质指标。轮作后期水芹(Oenanthe stolonifera)种植能降低甘露青鱼养殖场TOC、NO_3^--N、TP等水质指标,降低苏州经济鱼亲本塘TOC、COD、NH_4^+-N、NO_3^--N、TP等水质指标。轮作前、后期均能降低底质TOC、TN和TP含量。"空心菜-水芹"轮作模式能显著降低养殖池塘水体中TOC、NH_4^+-N、NO_3^--N、TP指标和底泥中TOC、TN、TP指标。

关键词：水质底泥轮作原位修复

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Research of the assessable method of postpartum hemorrhage

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TECHNOLOGY AND HEALTH CARE 2016年第Suppl 2期24卷 S465-S469页

作者： Chi, Zhenyu Zhang, song Wang, Yang Yang, Lin Yang, Yimin Li, xuwen Beijing Univ Technol Coll Life Sci & Bioengn Beijing Peoples R China Shenzhen Huada Gene Res Inst Shenzhen Guangdong Peoples R China

BACKGROUND: Postpartum hemorrhage (PPH) is the most common cause of parturient death worldwide [1]. However, most cases of PPH can be avoided. OBJECTIVE: This paper employs statistical methods to screen risk factors of PPH and calculate relevant parameters. METHODS: Multivariable logistic regression analysis was applied to obtain the regression equation and odds ratio (OR) value. The determined risk factors were assigned comprehensive and reasonable scores according to scientific relevance and reasoning according to the OR value. P-PPH values were calculated in order to assess the morbidity of PPH. RESULTS: The scores of pregnant women could be intuitively used to show the risk of getting PPH. CONCLUSIONS: Through the above methods, a comprehensive risk evaluation method of detecting PPH was developed.

关键词： Postpartum hemorrhage high risk factors comprehensive scores

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Comparison of electrohysterogram signal measured by surface electrodes with different designs: A computational study with dipole band and abdomen models

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SCIENTIFIC REPORTS 2017年第1期7卷 17282-17282页

作者： Gao, Pei Hao, Dongmei An, Yang Wang, Ying Qiu, Qian Yang, Lin Yang, Yimin Zhang, song Li, xuwen Zheng, Dingchang Beijing Univ Technol Coll Life Sci & Bioengn Beijing 100124 Peoples R China Anglia Ruskin Univ Hlth & Wellbeing Acad Fac Med Sci Chelmsford CM1 1SQ Essex England

Non-invasive measurement of uterine activity using electrohysterogram (EHG) surface electrodes has been attempted to monitor uterine contraction. This study aimed to computationally compare the performance of acquiring EHG signals using monopolar electrode and three types of Laplacian concentric ring electrodes (bipolar, quasi-bipolar and tri-polar). With the implementation of dipole band model and abdomen model, the performances of four electrodes in terms of the local sensitivity were quantified by potential attenuation. Furthermore, the effects of fat and muscle thickness on potential attenuation were evaluated using the bipolar and tri-polar electrodes with different radius. The results showed that all the four types of electrodes detected the simulated EHG signals with consistency. That the bipolar and tri-polar electrodes had greater attenuations than the others, and the shorter distance between the origin and location of dipole band at 20 dB attenuation, indicating that they had relatively better local sensitivity. In addition, ANOVA analysis showed that, for all the electrodes with different outer ring radius, the effects of fat and muscle on potential attenuation were significant (all p < 0.01). It is therefore concluded that the bipolar and tri-polar electrodes had higher local sensitivity than the others, indicating that they can be applied to detect EHG effectively.

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杂草稻灌浆期剑叶的叶绿素荧光特性

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植物生理学报 2017年第9期53卷 1742-1752页

作者：徐文蓉赵灿强胜戴伟民宋小玲南京农业大学杂草研究室南京210095

杂草稻(Oryza sativa ***)是与水稻同属同种的严重影响水稻产量和品质的杂草。杂草稻的早熟特性是其造成严重危害的重要原因之一。目前杂草稻的早熟现象虽有报道,但机制尚不明确。本研究以扬州、泰州、茂名和丹东地区的杂草稻与栽培稻... 详细信息

杂草稻(Oryza sativa ***)是与水稻同属同种的严重影响水稻产量和品质的杂草。杂草稻的早熟特性是其造成严重危害的重要原因之一。目前杂草稻的早熟现象虽有报道,但机制尚不明确。本研究以扬州、泰州、茂名和丹东地区的杂草稻与栽培稻为试验材料,同质园下比较了杂草稻与栽培稻从播种到抽穗、从抽穗到完熟(灌浆期)所需天数的差异,在灌浆期剑叶的叶绿素含量、叶绿素荧光参数,分析它们和杂草稻早熟的关系。结果显示:杂草稻比相应栽培稻从抽穗到完熟所需时间短10～33 d。在灌浆过程中,杂草稻剑叶的叶绿素含量低,或下降速度快于栽培稻,存在早衰现象。杂草稻剑叶的光合性能指数PI_(ABS)、最大光化学效率(φ_(Po))、活性反应中心数(RC/ABS)、用于电子传递的量子产额(φ_(Eo))和光系统II(PSII)受体侧相对电子传递速率(Ψ_0)均在抽穗期最高,整体呈逐渐降低的趋势。栽培稻则大多在乳熟期最高,呈先上升后降低的趋势,但其下降速度快于杂草稻。剑叶的早衰、达到PSII最大光合性能时间提前可能是杂草稻早熟的机制之一。

关键词：杂草稻叶绿素含量快速叶绿素荧光早熟

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Development of a new characteristic parameter - waveform index of finger blood volume pulse

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Computer Assisted Surgery 2016年第sup1期21卷 6-10页

作者： Wu, Wenjie Yang, Lin Zhang, song Hao, Dongmei Yang, Yimin Li, xuwen Wu, Guanghui Zhang, Lei Luo, Zhichang Beijing Univ Technol Coll Life Sci & Bioengn 100 Ping Le Yuan Beijing 100124 Peoples R China Beijing Anzhen Hosp Beijing Peoples R China Beijing Yes Med Devices Co Ltd Beijing Peoples R China

Background: Finger blood volume pulse (F-BVP) contains abundant human cardiovascular system information, including information regarding circulation in the heart, blood vessel function, and the microcirculation system. Pulse waveform analysis is a method for analyzing F-BVP. Methods and results: A new parameter of F-BVP, called waveform index (WI), was extracted, defined, subsequently compared between groups of different cardiovascular function, and compared with age and systolic blood pressure. WI values of the negative and positive groups were 0.209 +/- 0.066 (p<.001) and 0.305 +/- 0.066 (p<.001), respectively. The classification threshold value of WI was 0.24, revealing a sensitivity of 77%, a specificity of 68% and a classification accuracy of 73%. The odds ratio (OR) was 7.164, indicating that subjects with WI > 0.24 had a 7.164 times greater risk of suffering from cardiovascular disease than of not suffering from cardiovascular disease. Age and systolic blood pressure had consistent correlation with WI, in addition, WI had the same trend with age and systolic blood pressure. Conclusion: Because WI of F-BVP was determined according to only F-BVP waveform and increases in age and systolic blood pressure, different values of WI represent different cardiovascular physiological conditions. To a certain extent, the changes of WI reflect the changes of human cardiovascular function. Therefore, WI has the potential to be widely used in disease screening and clinical practice.

关键词： Finger blood volume pulse waveform index cardiovascular function classification threshold value

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Computerized analysis of acceleration parameter for the non-stress test normal and potentially abnormal fetuses

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Computer Assisted Surgery 2016年第sup1期21卷 1-5页

作者： Li, Guangfei Zhang, song Yang, Lin Li, Shufang Wang, Yan Hao, Dongmei Yang, Yimin Li, xuwen Zhang, Lei Xu, Mingzhou Beijing Univ Technol Coll Life Sci & Bioengn Beijing Peoples R China Peking Univ Hosp 3 Obstet & Gynecol Beijing Peoples R China Beijing Yes Med Devices Co Ltd Beijing Peoples R China Beijing Aerosp Changfeng Co Ltd Beijing Peoples R China

Non-stress testing (NST) is the primary method of determining fetal condition during the perinatal period, and as such, has high specificity. However, short-term monitoring and visual inspection of the cardiotocogram demonstrates several limitations in understanding fetal status which can be mistaken as predictors of neonatal asphyxia. Fetal electrocardiography (FECG) is a novel, long-term monitoring method which can reflect more objective and accurate fetal information. This article presents experimental results of four fetal heart rate (FHR) acceleration features of 44 fetuses extracted from FECG. The novelty of this approach lies in its combined use of parameters which can express both duration and amplitude of heart rate acceleration. Results demonstrate that most parameters significantly differ between normal fetuses and fetuses with suspected abnormalities. Results are promising for the identification of a set or parameters which may be used as classifiers to improve the success rate when distinguishing between normal and abnormal fetuses.

关键词： Fetal heart rate acceleration FECG gestational age

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结肠憩室炎CT分级及其对临床治疗方案的预测

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中国医学影像技术 2017年第11期33卷 1670-1673页

作者：郭威曾旭文梁治平陈松周洁李艳梅何敏聪暨南大学附属广州红十字会医院广州市红十字会医院放射科广东广州510220

目的探讨结肠憩室炎CT分级及其对临床治疗方案的预测意义。方法回顾性分析41例已确诊为结憩室炎的患者的CT资料并对其进行CT影像学分级,1~3级预测为可行保守治疗,4~5级预测为需行手术治疗,并与临床实际治疗方案进行对照。结果结肠憩室... 详细信息

目的探讨结肠憩室炎CT分级及其对临床治疗方案的预测意义。方法回顾性分析41例已确诊为结憩室炎的患者的CT资料并对其进行CT影像学分级,1~3级预测为可行保守治疗,4~5级预测为需行手术治疗,并与临床实际治疗方案进行对照。结果结肠憩室炎CT主要表现为突出于肠壁的小囊袋状突起,憩室壁及邻近肠壁水肿增厚(40/41,97.56%)、周围脂肪结构紊乱及邻近腹膜增厚(39/41,97.12%)。41例憩室炎患者术前CT分级预测治疗方案的准确率为85.37%(36/41)。结论结肠憩室炎的CT表现有一定的特征性,CT分级有助于临床制定治疗方案。

关键词：憩室炎结肠体层摄影术 X线计算机

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