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SSRN 2024年

作者： Shen, Yuanyuan Li, Kai Zhang, Wenkai Li, xuwen song, Jian Dong, Yaohua Dong, Lihua Institute of Marine Materials Science and Engineering College of Ocean Science and Engineering Shanghai Maritime University Shanghai201306 China Shanghai Spaceflight Precision Machinery Institute Shanghai201600 China

The microbial diversity and the effect of microorganism on cutting fluids were analyzed. The surface cutting fluid from the machine tool tank was dominated by aerobic bacteria, while the bottom mainly harbored anaerobic and facultative microorganisms, with Yarrowia lipolytica being the dominant fungus. The obligate anaerobic bacteria were present in the cutting fluid. The secretion of acidic substances and the consumption of triethanolamine and rganophosphate esters led to a significant decrease in the cutting fluid pH and corrosion inhibition capability. Moreover, during the late stage, the number of fungi increased. The resulting fungal mycelium and porous extracellular polymeric substance secreted by microorganisms caused flocculation of the effective components in the cutting fluid, resulting in reduced lubricity and poor stability. © 2024, The Authors. All rights reserved.

关键词： Mycelium

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Rapid analysis of soluble solid content in navel orange based on visible-near infrared spectroscopy combined with a swarm intelligence optimization method

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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2020年第0期228卷 117815-000页

作者： song, Jie Li, Guanglin Yang, Xiaodong Liu, xuwen Xie, Lin Southwest Univ Coll Engn & Technol Chongqing 400715 Peoples R China

Navel orange is a very popular fruit which is rich in nutrition necessary to human health. Nowadays, rapid, nondestructive and pollution-free analysis of internal organic compounds of fruit is an important and promising technology. The purpose of this paper is to present a swarm intelligence optimization method to extract the feature information of visible-near infrared (Vis-NIR) spectra of navel orange for rapid and nondestructive analysis of soluble solid content (SSC) in navel orange. This method was developed on particle swarm optimization (PSO) and named as piecewise particle swarm optimization (PPSO). The experimental results showed that the PPSO algorithm proposed in this paper overcame the disadvantage of PSO's premature convergence. The PLS model based on variables selected by PPSO for nondestructively detecting SSC of navel orange yield promising results, as the standard deviation of prediction (SEP) was 0.427 degrees Brix while the standard error of laboratory (SEL) was 0.22 degrees Brix. It indicated that the application of near infrared spectroscopy (NIRS) technology combined with PPSO for rapid analysis of soluble solid content in navel orange was feasible. (C) 2019 Elsevier B.V. All rights reserved.

关键词： Piecewise PSO Wavelength variables selection NIR SSC Navel orange

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End-to-End Individual Pig Detection Based on Transfer Learning

End-to-End Individual Pig Detection Based on Transfer Learni...

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Pattern Recognition and Artificial Intelligence (PRAI), International Conference on

作者： Zihan Huang Hao Li Xiaorui Xiong xuwen He Rui song Mengzhen Huang School of Mathematics and Computer Science Wuhan Polytechnic University Wuhan China

Nowadays, monitoring animals manually on a large scale is challenging, while external devices can provide more prosperous and accurate information. Therefore, there are already applications incorporating wearable sensors to monitor individual pigs. With continued breakthroughs in computer vision, low-cost visible light cameras can help monitor pigs. CNN-based object detection methods have been applied to individual pig detection, but most rely on hand-designed components and require prior knowledge. Based on previous researchers' progress, we propose using DETR, an end-to-end object detection method, to detect individual pigs. Also, to adapt to our natural pig farm environment, we trained the model with transfer learning to achieve an AP of 0.995 on our collected dataset.

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Mechanistic analysis of the coordination between MbEGS4 and MbEOMT in methyleugenol synthesis by Melaleuca bracteata F. Muell

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INDUSTRIAL CROPS AND PRODUCTS 2024年 222卷

作者： Xiang, xuwen song, Keke Lin, Yongsheng You, Jingnan Xu, Yan Niu, Xianqian Zheng, Guohua Wu, Yingxiang Pan, Heli Li, Yongyu Fujian Agr & Forestry Univ Coll Hort Key Lab Minist Educ Genet Breeding & Multiple Utilizat Cro Fuzhou 350002 Peoples R China Fujian Agr & Forestry Univ Inst Nat Prod Hort Plants Fuzhou 350002 Peoples R China Fujian Agr & Forestry Univ Coll Life Sci Fuzhou 350002 Peoples R China Fujian Agr & Forestry Univ Coll Landscape Architecture & Art Fuzhou 350002 Peoples R China Fujian Inst Trop Crops Zhangzhou 363001 Peoples R China Qingyuan Agr Sci & Technol Extens Serv Ctr Qingyuan 511518 Peoples R China

Methyleugenol is widely found in essential oils from aromatic plants, attracting insects for pollination and possessing antimicrobial and antioxidant features. Methyleugenol content in the essential oil from Melaleuca bracteata leaves is 90.46 %, making it an excellent material for assessing methyleugenol's biosynthetic pathway. Eugenol synthase (EGS) and eugenol-o-methyltransferase (EOMT) represent key genes in methyleugenol's biosynthetic pathway but their respective functions are unclear. In this study, overexpression and virus-induced gene silencing (VIGS) techniques were applied to investigate the major regulatory gene among MbEGS1, MbEGS2, MbEGS3, and MbEGS4 in the biosynthesis pathway of methyleugenol. We also aimed to examine the coordination mechanism between EGS and EOMT in methyleugenol production by M. bracteata. The data revealed MbEGS4 transcription and methyleugenol content were most significantly increased after overexpression of the MbEGS1-4 gene in M. bracteata and Fragaria vesca. Conversely, MbEGS4 transcription and methyleugenol content were most significantly reduced after MbEGS1-4 gene silencing by VIGS in M. bracteata. These results underscore a major regulatory role for the MbEGS4 gene in the biosynthetic pathway of methyleugenol in M. bracteata. Remarkably, upon overexpression and silencing of MbEOMTs, the transcription levels of MbEGSs were notably upregulated, with MbEGS4 showing the highest increase. Collectively, these findings suggested the presence of a specific transcription factor bound to the promoters of MbEGS and MbEOMT, inducing their expression for the synergistic and efficient production of methyleugenol in M. bracteata.

关键词： Phenylpropene Methyleugenol Biosynthesis Eugenol synthase gene

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土壤中微囊藻毒素的污染特征及其对作物的危害

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农业环境科学学报 2025年第3期44卷 594-604页

作者：汪彤馨宋伟杰周娈陈旭文高彦征南京农业大学土壤有机污染控制与修复研究所南京210095

在蓝藻水华发生过程中常伴随着微囊藻毒素(MCs)的产生。MCs经灌溉、溢流、藻肥施用等方式进入土壤后可被农作物吸收富集,并通过食物链进入人体,严重威胁环境和人体健康。本文分析了土壤中MCs的来源和污染特征,发现灌溉是MCs进入土壤的... 详细信息

在蓝藻水华发生过程中常伴随着微囊藻毒素(MCs)的产生。MCs经灌溉、溢流、藻肥施用等方式进入土壤后可被农作物吸收富集,并通过食物链进入人体,严重威胁环境和人体健康。本文分析了土壤中MCs的来源和污染特征,发现灌溉是MCs进入土壤的主要途径,土壤中MCs的累积量高达276μg·kg^(-1),而且土壤中MCs污染具有普遍性、稳定性以及难去除等特点。本文探究了MCs在农田土壤与粮食、蔬菜作物中的累积、迁移及危害,发现土壤中MCs在作物生长的各个时期均可被吸收累积,其在青菜、油菜中的累积量较高,尤其在成熟作物的可食用部位。MCs在作物体内的累积呈现根>叶>茎的规律。MCs会降低作物种子的发芽率,抑制个体生长,使个体形态发生改变,以及通过降低超氧化物歧化酶、过氧化物酶等酶的活性使生理生化性质发生改变。此外,MCs对作物幼苗的影响大于成熟植株。文章提出了根际效应可能是影响MCs从土壤到作物迁移的主要原因,是未来值得关注的研究方向,该研究为探索MCs的污染问题以及减少其环境风险提供了新的思路。

关键词：蓝藻水华土壤污染蔬菜根际效应迁移

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Screening and Expression Analysis of POD Gene in POD-H2O2 Pathway on Bud Dormancy of Pear (Pyrus pyrifolia)

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FORESTS 2024年第3期15卷 434页

作者： Xiang, xuwen song, Keke Li, Yinyin Zhang, Chenyu Zhou, Ruiqi Feng, Yu You, Jingnan Wu, Jingdong Zhang, Yanhui Jiang, Cuicui Li, Yongyu Fujian Agr & Forestry Univ Coll Hort Key Lab Minist Educ Genet Breeding & Multiple Utilizat Cro Fuzhou 350002 Peoples R China Fujian Agr & Forestry Univ Inst Nat Prod Hort Plants Fuzhou 350002 Peoples R China Agr & Rural Bur Jianning Cty Econ Crop Stn Sanming 354500 Peoples R China Agr & Rural Bur Yongtai Cty Econ Crop Stn Fuzhou 350700 Peoples R China Fujian Acad Agr Sci Fruit Res Inst Fuzhou 350002 Peoples R China

Bud endodormancy represents a pivotal and intricate biological process influenced by both genetic and epigenetic factors, the exact mechanism of which remains elusive. Hydrogen peroxide (H2O2) functions as a signalling molecule in the regulation of dormancy, with peroxidase (POD) playing a crucial role in governing H2O2 levels. Our prior transcriptomic and metabolomic investigations into diverse pear dormancy phases posited that POD predominantly oversees pear bud dormancy. In this study, we utilised qRT-PCR to screen the most significantly expressed gene, Pyrus pyrifolia POD4-like (PpPOD4-like), from seven POD genes. Subsequently, H2O2 test kits, overexpression methods, and subcellular localisation techniques were employed to assess changes in H2O2 content, POD activity, PpPOD4-like expression, and its cellular positioning during pear bud dormancy. Subcellular localisation experiments revealed that PpPOD4-like is situated on the cell membranes. Notably, H2O2 content exhibited a rapid increase during endodormancy and decreased swiftly after ecodormancy. The fluctuation pattern of POD activity aligned with that of H2O2 content. Additionally, PpPOD4-like expression was markedly upregulated, displaying an overall upward trajectory. Our findings indicate that PpPOD4-like modulates H2O2 levels by regulating POD activity, thereby actively participating in the intricate regulation of pear dormancy processes.

关键词： endodormancy overexpression H2O2 POD genes

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Recent advances in the synthesis of biodegradable polyesters by sustainable polymerization: lipase-catalyzed polymerization

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RSC ADVANCES 2020年第59期10卷 36230-36240页

作者： Liu, Ying song, Lijie Feng, Na Jiang, Wei Jin, Yongri Li, xuwen Jilin Univ Coll Chem 2699 Qianjin Rd Changchun 130012 Jilin Peoples R China Jilin Prov Acad Tradit Chinese Med Clin Hosp 1 Changchun 130021 Peoples R China Zhengzhou Univ Acad Med Sci Dept Mol Pathol Applicat Ctr Precis MedAffiliated Hosp 2 Zhengzhou 450052 Henan Peoples R China

Over the past three decades, enzymatic polymerization has dramatically developed and gradually broadened as a creative methodology in the construction of polymeric materials with tailor-made structures and properties. Compared with transition metal catalyst polymerizations, enzymatic polymerization is more attractive in the biomedicine field due to the metal-free residue, good biocompatibility, and few by-products. Meanwhile, enzymatic polymerization has far more activity towards macrolides. In this review, the synthesis of lipase-catalyzed polymer materials is systematically summarized, focusing on the synthesis of the complex and well-defined polymers. The enzymatic polyester synthesis was then discussed concerning the different reaction types, including ring-opening polymerization, polycondensation, a combination of ring-opening polymerization with polycondensation, and chemoenzymatic polymerization. Besides, exploration of novel biocatalysts and reaction media was also described, with particular emphasis on the enzymes obtainedviaimmobilization or protein engineering strategies, green solvents, and reactors. Finally, recent developments in catalytic kinetics and mechanistic studies through the use of spectroscopy, mathematics, and computer techniques have been introduced. Besides, we addressed the remaining central issues in enzymatic polymerization and discussed current studies aimed at providing answers.

关键词： Ring opening polymerization

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Research on the Application of BIM Technology in Construction Cost Management

Research on the Application of BIM Technology in Constructio...

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作者： Zhi Xu Xingqu Gao Zhongquan song xuwen Tian Xiaohan Jiang Harbin Far Eastinstitute of Technology Northeast Forestry University Engineering Consulting and Design Institute

In the traditional project cost management, due to the influence of many factors, its calculation accuracy is not high enough, which brings great difficulties to the construction project cost management. Using BIM technology to build a cost management model can provide very intuitive analysis results for cost managers, thus providing strong technical support for cost management of construction projects. This paper analyzes the application of BIM technology in construction cost management.

关键词： Construction engineering Cost management Technical BIM

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Retracted on February 24, 2022: The Order and Disorder of Higher Education System 21

Retracted on February 24, 2022: The Order and Disorder of Hi...

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作者： xuwen Zhang Jingming Chi Jing song Dalian University of Technology China Liaoning Police College China

ISBN: (纸本)9781450385015

NOTICE OF RETRACTION: While investigating potential publication-related misconduct in connection with the ICIMTech 2021 Conference Proceedings, serious concerns were raised that cast doubt on the integrity of the peer-review process and all papers published in the Proceedings of this Conference. The integrity of the entire Conference has been called into question. As a result, of its investigation, ACM has decided to retract the Entire Conference Proceedings and all related papers from the ACM Digital *** of the papers from this Proceeding should be cited in the literature because of the questionable integrity of the peer review process for this Conference.

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窄蒂鼻唇沟皮下蒂皮瓣在鼻部及鼻旁恶性肿瘤切除后创面修复中的应用

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中华解剖与临床杂志 2023年第8期28卷 527-532页

作者：李旭文葛秀玉宋培军徐静蚌埠医学院第一附属医院整形外科蚌埠233004

目的探讨窄蒂鼻唇沟皮下蒂皮瓣在鼻部及鼻旁恶性肿瘤切除术后修复面部缺损的效果。方法回顾性队列研究。纳入蚌埠医学院第一附属医院整形外科2018年6月—2022年6月收治的鼻部及鼻旁体表恶性肿瘤患者40例,其中男13例、女27例,年龄52~93(6... 详细信息

目的探讨窄蒂鼻唇沟皮下蒂皮瓣在鼻部及鼻旁恶性肿瘤切除术后修复面部缺损的效果。方法回顾性队列研究。纳入蚌埠医学院第一附属医院整形外科2018年6月—2022年6月收治的鼻部及鼻旁体表恶性肿瘤患者40例,其中男13例、女27例,年龄52~93(69±12)岁。患者均行肿瘤扩大切除术,根据肿瘤切除后创面修复方法不同分为2组:对照组20例,采用传统的局部转位皮瓣修复;观察组20例,采用窄蒂鼻唇沟皮下蒂推进皮瓣修复。观察并比较2组患者的临床基线资料,皮瓣面积、皮瓣蒂部宽度、皮瓣面积与蒂部宽度比值、手术时间、术中出血量、皮瓣存活率、供瓣区直接缝合率等围术期指标,术后6个月温哥华瘢痕量表(VSS)评分,以及术后创面感染、皮瓣下积液、创面出血、皮瓣对周围组织牵拉、蒂部“猫耳”形成等并发症发生情况。结果2组患者年龄、性别、住院时间以及肿瘤性质、大小、部位等基线资料比较,差异均无统计学意义(P值均>0.05)。观察组皮瓣面积为(6.90±1.94)cm^(2),对照组为(7.80±2.24)cm^(2),差异无统计学意义(t=1.36,P=0.182)。观察组皮瓣蒂部宽度(0.98±0.29)cm、皮瓣面积与蒂宽比值7.35±2.75、手术时间(70.5±16.7)min、术中出血量(47.5±8.5)mL,对照组依次为(2.93±0.65)cm、2.75±0.82、(83.5±19.9)min、(60.5±15.0)mL,组间比较差异均有统计学意义(t=12.25、7.17、2.24、3.36,P值均<0.05)。观察组与对照组皮瓣存活率分别为95.0%(19/20)和90.0%(18/20),差异无统计学意义(χ^(2)=0.00,P=1.000)。观察组供瓣区直接缝合率为95.0%(19/20),高于对照组的60.0%(12/20),差异有统计学意义(χ^(2)=5.16,P=0.023)。术后6个月观察组血管循环VSS评分为(1.40±0.99)分,对照组为(1.65±0.81)分,差异无统计学意义(t=0.87,P=0.388);观察组皮瓣色泽、柔软度、厚度VSS评分及VSS总分分别为(0.90±0.64)、(2.00±1.03)、(1.30±0.92)、(5.60±3.27)分,对照组依次为(1.45±0.76)、(2.65±0.81)、(1.95±0.89)、(7.70±2.66)分,差异均有统计学意义(t=2.48、2.22、2.27、2.23,P值均<0.05)。观察组与对照组术后发生皮瓣对周围组织牵拉分别为7例和14例、蒂部猫耳形成分别为5例和16例,组间比较差异均有统计学意义(χ^(2)=4.91、12.13,P值均<0.05)。2组患者术后切口感染、出血、皮瓣下积液发生率比较,差异均无统计学意义(P值均>0.05)。结论采用窄蒂鼻唇沟皮下蒂皮瓣修复鼻部及鼻旁恶性肿瘤切除后的软组织缺损创面,对蒂部及供瓣区的处理优于传统的局部邻近皮瓣,且有效缩短手术时间、减少出血量、提高术后皮瓣美观度、减少并发症发生。

关键词：外科皮瓣窄蒂鼻唇沟皮下蒂皮瓣鼻部及鼻旁恶性肿瘤创面修复

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