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振动与冲击 2023年第20期42卷 293-299页

作者：陈宇谢明亮景旭文邹晓峰刘金锋闫德俊江苏科技大学机械工程学院江苏镇江212100 中船黄埔文冲船舶有限公司广东省舰船先进焊接技术企业重点实验室广州510720

为研究齿轮间接触碰撞及啮合间隙等对舰炮随动系统性能及振动特性的影响,建立了考虑齿轮啮合、随动系统等多非线性因素的舰炮机电液耦合动力学模型。其中,基于改进的Lankarani-Nikravesh接触算法及齿轮间时变啮合刚度计算方法,建立了考... 详细信息

为研究齿轮间接触碰撞及啮合间隙等对舰炮随动系统性能及振动特性的影响,建立了考虑齿轮啮合、随动系统等多非线性因素的舰炮机电液耦合动力学模型。其中,基于改进的Lankarani-Nikravesh接触算法及齿轮间时变啮合刚度计算方法,建立了考虑时变啮合刚度的齿轮传动多体接触动力学模型。数值计算结果表明,随动系统中齿轮啮合会使舰炮的振动频率变高,降低随动系统的控制性能,但比例积分微分(proportional-integral-derivative,PID)控制器可在一定程度上抑制齿轮间接触非线性的不利影响。该研究可为舰炮炮控性能的预测和优化提高提供参考。

关键词：舰炮随动系统齿轮啮合间隙接触动力学仿真

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Co-regulation of indole glucosinolates and camalexin biosynthesis by CPK5/CPK6 and MPK3/MPK6 signaling pathways^°°

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Journal of Integrative Plant Biology 2020年第11期62卷 1780-1796页

作者： Liuyi Vang yan Zhang Rongxia Guan Sen Li xuwen Xu Shuqun Zhang Juan Xu State Key Lab oratory of Plant Physiology and Biochem istry College of Life SciencesZhejiang UniversityHangzhouZhejiang 310058China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Division of Biochemistry University of MissouriColumbiaMO 65211USA

Secondary plant metabolites,represented by indole glucosinolates(IGS)and camalexin,play important roles in Arabidopsis ***,we demonstrated the importance of MPK3 and MPK6,two closely related MAPKs,in regulating Botrytis cinerea(Bc)-induced IGS and camalexin *** we report that CPK5 and CPK6,two redundant calcium-dependent protein kinases(CPKs),are also involved in regulating the biosynthesis of these secondary *** loss-of-function of both CPK5 and CPK6 compromises plant resistance to *** profiling of CPK5-VK transgenic plants,in which a truncated constitutively active CPK5 is driven by a steroidinducible promoter,revealed that biosynthetic genes of both IGS and camalexin pathways are coordinately upregulated after the induction of CPK5-VK,leading to high-level accumulation of camalexin and 4-methoxyindole-3-ylmethylglucosinolate(4MI3G).Induction of camalexin and 4MI3G,as well as the genes in their biosynthesis pathways,is greatly compromised in cpks cpk6 mutant in response to *** a conditional cpk5 cpk6 mpk3 mpk6 quadruple mutant,Be resistance and induction of IGS and camalexin are further reduced in comparison to either cpks cpk6 or conditional mpk3 mpk6 double mutant,suggesting that both CPK5/CPK6 and MPK3/MPK6 signaling pathways contribute to promote the biosynthesis of 4MI3G and camalexin in defense against Be.

关键词： indole coordinate constitutive

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Single-Atom Fe-Anchored Nano-Diamond With Enhanced Dual-Enzyme Mimicking Performance for H2O2 and Glutathione Detection

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FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 2022年 9卷 790849页

作者： Liu, Ying yan, Jianghong Huang, Yu Sun, Zhiheng Zhang, Huijing Fu, Lihaoyuan Li, xuwen Jin, Yongri Jilin Univ Coll Chem Changchun Peoples R China First Clin Hosp Jilin Prov Acad Tradit Chinese Med Changchun Peoples R China

Glutathione (GSH) is an important antioxidant and free radical scavenger that converts harmful toxins into harmless substances and excretes them out of the body. In the present study, we successfully prepared single-atom iron oxide-nanoparticle (Fe-NP)-modified nanodiamonds (NDs) named Fe-NDs via a one-pot in situ reduction method. This nanozyme functionally mimics two major enzymes, namely, peroxidase and oxidase. Accordingly, a colorimetric sensing platform was designed to detect hydrogen peroxide (H2O2) and GSH. Owing to their peroxidase-like activity, Fe-NDs can oxidize colorless 3,3 ',5,5 '-tetramethylbenzidine (TMB) into blue with sufficient linearity at H2O2 concentrations of 1-60 mu M and with a detection limit of 0.3 mu M. Furthermore, using different concentrations of GSH, oxidized TMB can be reduced to TMB, and the color change from blue to nearly colorless can be observed by the naked eye (linear range, 1-25 mu M;detection limit, 0.072 mu M). The established colorimetric method based on oxidase-like activity can be successfully used to detect reduced GSH in tablets and injections with good selectivity and high sensitivity. The results of this study exhibited reliable consistency with the detection results obtained using high-performance liquid chromatography (HPLC). Therefore, the Fe-NDs colorimetric sensor designed in this study offers adequate accuracy and sensitivity.

关键词： nanodiamond single-atom Fe peroxidase-like and oxidase-like activity colorimetric sensor glutathione

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人工养殖黄河鲤形态特征、染色体核型及肌肉乳酸脱氢酶的电泳分析

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南方农业学报 2019年第8期50卷 1844-1850页

作者：秦改晓张潇齐子鑫唐国盘王新华徐文彦郭国军河南牧业经济学院动物科技学院

【目的】从形态特征、染色体核型和生化遗传3个层面评估人工养殖黄河鲤(Cyprinus carpio haematopterus)种质资源状况,为探讨种质鉴定在黄河鲤资源保护和良种选育中的应用提供参考依据。【方法】采用形态学方法测定30尾人工养殖黄河鲤... 详细信息

【目的】从形态特征、染色体核型和生化遗传3个层面评估人工养殖黄河鲤(Cyprinus carpio haematopterus)种质资源状况,为探讨种质鉴定在黄河鲤资源保护和良种选育中的应用提供参考依据。【方法】采用形态学方法测定30尾人工养殖黄河鲤样品的可量性状和可数性状,通过聚丙烯酰胺凝胶垂直板电泳检测其肌肉乳酸脱氢酶(LDH)的表达情况,并借助肾细胞滴片—空气干燥法对人工养殖黄河鲤的染色体数目及其核型进行分析。【结果】人工养殖黄河鲤体形呈梭形,头小而腹部圆,口端位,呈马蹄形,须2对;体侧鳞片呈金黄色,腹部色淡而白,臀鳍、尾柄及尾鳍下叶为橙红色,胸鳍和腹鳍呈桔黄色;鳍式为背鳍D.ⅲ-16~20、臀鳍A.ⅲ-5;齿式为1·1·3/3·1·1;左侧第一鳃弓外侧鳃耙数为19~25条。人工养殖黄河鲤肌肉LDH酶带条数分为2条和5条两种类型。人工养殖黄河鲤染色体数目2n=100,染色体相对长度范围在1.20%~2.96%,其染色体核型公式为18m+38sm+22st+22t,臂数(NF)=156。【结论】人工养殖黄河鲤肌肉LDH出现与种质标准SC 1041—2001《黄河鲤》中不同的条带类型,推测黄河鲤人工养殖群体已出现种质混杂现象。因此,在黄河鲤品种改良或增殖放流前必须对其种质状况进行系统鉴定和评价,原良种场在制种保种过程中应加强不同类养殖群体的隔离或防止混杂,以确保黄河鲤种质纯正。

关键词：黄河鲤形态特征染色体核型乳酸脱氢酶(LDH)

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蒙古红鲌形态特征及其两种同工酶的电泳分析

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南方农业学报 2019年第1期50卷 194-200页

作者：秦改晓齐子鑫唐国盘王新华徐文彦郭国军路畅河南牧业经济学院动物科技学院郑州450046

【目的】从形态特征和生化遗传两个角度丰富蒙古红鲌(Erythroculter mongolicus)种质资源的研究内容,筛选出蒙古红鲌的特征生化遗传参数,为其种质标准制定、种质资源保护及良种选育提供理论依据。【方法】采用传统形态学方法测定蒙古红... 详细信息

【目的】从形态特征和生化遗传两个角度丰富蒙古红鲌(Erythroculter mongolicus)种质资源的研究内容,筛选出蒙古红鲌的特征生化遗传参数,为其种质标准制定、种质资源保护及良种选育提供理论依据。【方法】采用传统形态学方法测定蒙古红鲌的可数和可量性状,并结合聚丙烯酰胺凝胶电泳(PAGE)检测蒙古红鲌心脏、眼睛晶状体、肌肉、肾脏及肝脏等5种组织中乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)的表达情况。【结果】蒙古红鲌的主要形态特征:头部背面平坦,口端位,下颌略比上颌长;腹棱自腹鳍基部至肛门;身体上半部浅棕色、下部银白色,胸腹鳍浅黄色,尾鳍下叶鲜红色;鳍式为背鳍D.ⅲ-7和臀鳍A.ⅲ-19～22;齿式为2·4·5(4)/5(4)·4·2或2·4·4/5·3·2:左侧第一鳃弓外侧鳃耙数为18～20。从蒙古红鲌心脏、眼睛晶状体、肌肉、肾脏和肝脏组织中均能检测到LDH和MDH,但不同组织中的酶带数目和活性各不同,呈明显的组织特异性,其中,LDH酶带条数分别为6、6、6、7和8条,MDH酶带条数分别为3、3、5、5和4条。各样本蒙古红鲌眼睛晶状体的LDH酶带数及其活性基本相同,均呈单态,表达稳定,且分离效果好。【结论】LDH和MDH在蒙古红鲌体内分布较广泛,且具有组织特异性,其中眼睛晶状体LDH表达丰富且活性稳定,可作为鉴定蒙古红鲌种质的特征生化遗传参数。

关键词：蒙古红鲌形态特征乳酸脱氢酶(LDH) 苹果酸脱氢酶(MDH) 组织特异性特征生化遗传参数

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Fabrication and properties of MEMS compatible energetic arrays based on carbon-based copper azide

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APPLIED SURFACE SCIENCE 2022年 577卷 150643-150643页

作者： Liu, xuwen Hu, yan Chen, Bingwen Wei, Hai Su, Jiaxin yang, Caimin Hu, Jiaheng Ye, Yinghua Shen, Ruiqi Nanjing Univ Sci & Technol Sch Chem & Chem Engn Nanjing 210094 Peoples R China Minist Ind & Informat Technol Micronano Energet Devices Key Lab Nanjing 210094 Peoples R China

Copper azide is a promising primary explosive with low toxicity and high energy density. However, due to the extreme electrostatic sensitivity, it does not have many practical applications in the miniaturized pyrotechnics. In this work, we constructed an energetic composite, embedding in oriented carbon nanotubes (CNTs) arrays grown on a silicon substrate, which is compatible with the micmfabrication technique of Micro-Electro-Mechanical Systems (MEMS). Since it has excellent electrical conductivity and mechanical strength, oriented CNTs, as nano-containers of Cu(N-3)(2), can availably reduce the electrostatic sensitivity and bring about the directional output of detonation energy. The oriented CNTs arrays were prepared by magnetron sputtering, anodization and chemical vapor deposition successively. Electrochemical deposition was used to deposit copper nanoparticles in the cavities of CNTs, which were converted into copper azide by subsequent in-situ azide reaction. The electrostatic sensitivity test, thermal performance analysis and laser ignition experiment were conducted on the energetic composite, respectively. The results show that the energetic composite kept safe under an electrostatic stimulation of 3.18 mJ, while original copper azide exploded when subjected to stimulation of 0.05 mJ. The energetic composite opens a new route of introducing hazardous primary explosives into micro-initiators and modern pyrotechnics.

关键词： Composite energetic materials Copper azide Oriented CNTs array Template method Electrochemical deposition Electrostatic safety

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Establishment of a personalized fetal growth curve model

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TECHNOLOGY AND HEALTH CARE 2021年第1_suppl期29卷 S311-S317页

作者： Bao, Xinyu Wang, yan Zhang, Song yang, Lin Liu, Guoli yang, Yimin Li, xuwen Hao, Dongmei Chen, Aiqing Liu, Xiaohong Shao, Jing Beijing Univ Technol Fac Environm & Life Sci Beijing 100124 Peoples R China Beijing Int Sci & Technol Cooperat Base Intellige Beijing 100124 Peoples R China Peking Univ Peoples Hosp Beijing 100044 Peoples R China Beijing Yes Med Devices Co Ltd Beijing 100152 Peoples R China

BACKGROUND: Fetal weight is one of the important indicators for judging whether fetal growth and development are normal. Fetal weight exceeding the normal range may lead to poor delivery outcomes. OBJECTIVE: We aimed to establish a personalized fetal growth curve in order to effectively monitor fetal growth during pregnancy. Fetal weight can be monitored while fetal growth and development are assessed. METHODS: This study retrospectively analyzed the birth weight and ultrasound database of 3,093 newborns delivered at normal term. The personalized fetal growth curve model was generated based on the birth weight formula established by Gardosi combined with the proportional weight equation. RESULTS: (1) The average birth weight of the single fetus at normal term was 3,457g. (2) According to the regression results of the proportion of fetal weight in full-term pregnancy and gestational week, the proportional weight equation is Weight% = 500.9 + 51.60GA + 1.727GA(2) 0.01718GA(3) (GA is gestational week), R-2 is 98%, P < 0.001. CONCLUSIONS: In this study, the normal birth weight of newborns and normal range of fetal weight can be estimated by using the personalized fetal growth curve model.

关键词： Fetal weight fetal growth curve birth weight proportional weight equation normal range

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The YDA-MKK4/MKK5-MPK3/MPK6 Cascade Functions Downstream of the RGF1-RGI Ligand-Receptor Pair in Regulating Mitotic Activity in Root Apical Meristem

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Molecular Plant 2020年第11期13卷 1608-1623页

作者： Yiming Shao Xinxing Yu xuwen Xu Yong Li Wenxin Yuan yan Xu Chuanzao Mao Shuqun Zhang Juan Xu State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhouZhejiang 310058China Division of Biochemistry University of MissouriColumbiaMO 65211USA Department of Molecular Cellular and Developmental BiologyYale UniversityNew HavenCT 06511USA

The mitotic activity of root apical meristem(RAM)is critical to primary root growth and *** studies have identified the roles of ROOT GROWTH FACTOR 1(RGF1),a peptide ligand,and its receptors,RGF1 INSENSITIVEs(RGIs),a clade of five leucine-rich-repeat receptor-like kinases,in promoting cell division in the RAM,which determines the primary root ***,the downstream signaling components remain *** this study,we identify a complete mitogen-activated protein kinase(MAPK or MPK)cascade,composed of YDA,MKK4/MKK5,and MPK3/MPK6,that functions downstream of the RGF1-RGI ligand-receptor *** to the rgi1/2/3/4/5 quintuple mutant,loss-of-function mutants of MPK3 and MPK6,MKK4 and MKK5,or YDA show a short-root phenotype,which is associated with reduced mitotic activity and lower expression of PLETHORA 1(PLT1)/PLT2 in the ***,MPK3/MPK6 activation in response to exogenous RGF1 treatment is impaired in the rgi1/2/3/4/5 quintuple,yda single,and mkk4 m kk5 double *** analyses demonstrated that the expression of constitutively active MKK4,MKK5,or YDA driven by the RGI2 promoter can rescue the short-root phenotype of the rgi1/2/3/4/5 *** together,these results suggest that the YDA-MKK4/MKK5-MPK3/MPK6 cascade functions downstream of the RGF1-RGI ligand-receptor pair and upstream of PLT1/PLT2 to modulate the stem cell population and primary root growth in Arabidopsis.

关键词： root meristem MPK3/MPK6 RGF1-RGIs PLT1/PLT2 signaling pathway Arabidopsis

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Fetal growth prediction: Establishing fetal growth prediction curves in the second trimester

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TECHNOLOGY AND HEALTH CARE 2021年第1_suppl期29卷 S345-S350页

作者： Wang, yan Bao, Xinyu Zhang, Song yang, Lin Liu, Guoli yang, Yimin Li, xuwen Hao, Dongmei Chen, Aiqing Liu, Xiaohong Shao, Jing Peking Univ Peoples Hosp Beijing 100044 Peoples R China Beijing Univ Technol Fac Environm & Life Sci Beijing 100124 Peoples R China Beijing Int Sci & Technol Cooperat Base Intellige Beijing 100124 Peoples R China Beijing Yes Med Devices Co Ltd Beijing 100152 Peoples R China

BACKGROUND: Monitoring fetal weight during pregnancy has a guiding role in prenatal care. OBJECTIVE: To establish a personalized fetal growth curve for effectively monitoring fetal growth during pregnancy. METHODS: (1) This study retrospectively analyzed the birth weight database of 2,474 singleton newborns delivered normally at term. The personalized fetal growth curve model was formed by combining the estimating birth weight of newborns with the proportional weight formula. (2) Multiple linear stepwise regression method was used to estimate the birth weight of newborns. RESULTS: (1) Delivery gestational age, weight at first visit, maternal height, pre-pregnancy body mass index, fetal sex, parity had significant effects on birth weight. Based on these parameters, the formula for calculating term optimal weight was obtained (R-2 = 22.8%, P < 0.001). (2) The personalized fetal growth curve was obtained according to the epidemiological factors input model of each pregnant woman. CONCLUSIONS: A model of personalized fetal growth curve can be established, and be used to evaluate fetal growth and development through estimated fetal weight monitoring.

关键词： Fetal weight growth charts personal health services

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骨肉瘤中PD-1和CTLA-4的表达与患者临床病理特征及预后的相关性

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肿瘤防治研究 2023年第1期50卷 63-68页

作者：闫广宁喻玲赖续文叶丹丽王蔚王卓才中国人民解放军南部战区总医院病理科广州510010

目的探讨PD-1和CTLA-4在骨肉瘤中的表达及其临床病理意义。方法收集2007—2016年南部战区总医院初诊初治骨肉瘤患者58例,运用免疫组织化学EnVision法检测PD-1、CTLA-4蛋白的表达。结果PD-1阳性31例(53.4%),阴性27例(46.6%);CTLA-4阳性19... 详细信息

目的探讨PD-1和CTLA-4在骨肉瘤中的表达及其临床病理意义。方法收集2007—2016年南部战区总医院初诊初治骨肉瘤患者58例,运用免疫组织化学EnVision法检测PD-1、CTLA-4蛋白的表达。结果PD-1阳性31例(53.4%),阴性27例(46.6%);CTLA-4阳性19例(32.8%),阴性39例(67.2%);PD-1和CTLA-4双阳性12例(20.7%),双阴性20例(34.5%),单阳性26例(44.8%)。PD-1阳性与是否行新辅助化疗、肿瘤复发和转移有关,PD-1阳性患者无病生存期及总生存期更短(P<0.05);CTLA-4阳性与Ennecking分期较晚有一定关联(P=0.051);PD-1和CTLA-4双阳性表达较双阴性和单阳性患者术后复发和转移比例显著升高(P<0.05),且生存期更短(P<0.05)。结论PD-1和CTLA-4阳性表达的患者预后不良,双阳性患者的预后较差,可作为骨肉瘤免疫治疗的有效依据。

关键词：骨肉瘤 PD-1 CTLA-4 预后

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