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中国组织工程研究 2023年第12期27卷 1884-1891页

作者：叶旭文顾勇陈亮苏州大学附属第一医院江苏省苏州市215000

背景:椎间盘退变机制复杂,炎症通路激活、炎症因子泛滥、活性氧过度积累等均可加速椎间盘退变进程。姜黄素是从中药材姜黄中提取的天然药物,具有抑制炎症、抗氧化应激等药理作用。目的:探讨负载姜黄素的缓释微球局部注射延缓大鼠椎间盘... 详细信息

背景:椎间盘退变机制复杂,炎症通路激活、炎症因子泛滥、活性氧过度积累等均可加速椎间盘退变进程。姜黄素是从中药材姜黄中提取的天然药物,具有抑制炎症、抗氧化应激等药理作用。目的:探讨负载姜黄素的缓释微球局部注射延缓大鼠椎间盘退变的疗效。方法:①采用微流控技术制备负载姜黄素的甲基丙烯酸酰化明胶缓释微球,评估其体外释药和降解情况;②将缓释微球与未载药微球分别与髓核细胞共培养,以单独培养的细胞为对照,将3组细胞分别培养于正常环境和氧化应激环境(加入H_(2)O_(2))中,通过CCK-8、活/死染色实验评估细胞的活性与增殖情况,qRT-PCR实验检测相关因子的表达水平;③将30只SD大鼠随机分为正常组、退变组、未载药微球组、姜黄素组、缓释微球组,每组6只,在大鼠尾椎7-8和8-9节段经皮穿刺建立椎间盘退变模型,未载药微球组、姜黄素组、缓释微球组退变的椎间盘内注射对应的溶液。4周后,通过影像学与组织学观察椎间盘组织结构改变。结果与结论:①缓释微球可缓慢持续释放姜黄素,至28 d时释放姜黄素总量为(84.11±2.71)%;在含有胶原酶的PBS中,缓释微球质量逐渐减小,至第5周时消失。②CCK-8和活/死染色实验显示,在正常环境下,缓释微球不会影响髓核细胞的增殖活力;在氧化应激环境下,相比未载药微球,缓释微球可维持髓核细胞的增殖活力。qRT-PCR实验显示,相较于正常环境,氧化应激环境激活了髓核细胞中多种炎症因子mRNA的过量表达;在氧化应激环境下,相较于未载药微球组与对照组,缓释微球组肿瘤坏死因子α、白细胞介素1β、白细胞介素6及基质金属蛋白酶3的mRNA表达降低,Ⅱ型胶原和蛋白聚糖的mRNA表达升高。③动物体内实验结果显示,未载药微球组4周后的影像学与组织学评估与退变组比较无明显差异;姜黄素组的椎间隙高度及MRI评分在第1周时较退变组和未载药微球组虽有一定程度改善,但在第4周时其影像学与组织学评估均不如缓释微球组。④结果表明,姜黄素缓释微球能够抑制H_(2)O_(2)诱导的髓核细胞凋亡、延缓椎间盘退变进程,其机制与抑制核因子κB通路、降低氧化应激水平有关。

关键词：微流控技术纳米粒微球冷冻干燥姜黄素椎间盘退变甲基丙烯酸酐化明胶

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Organic metal matrix Mil-88a nano-enzyme for joint repair in the osteoarthritis mouse model

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FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 2023年 11卷 1164942页

作者： Hu, Hao Huang, Xu Dai, Yankun Zhu, Kairun ye, xuwen Meng, Shengdong Zhang, Qing Xie, Xueguan Huaian Second Peoples Hosp Huaian Peoples R China

Introduction: In this paper we tried to conduct a novel nanomaterial strategy to overcome osteoarthritis (OA) in a mouse model. Methods: In this regard, after synthesizing the Mil-88a nanozyme, as a certain Fe-MOF, its toxic effects were detected by CCK-8 method and live-dead staining. The OA model of mouse was constructed, and paraffin sections of joints were taken for histological evaluation. In addition, immunofluorescence and immunohistochemistry were used to identify the OA progression and OARSI was used to evaluate the OA grades. We observed that Mil-88a could be easily synthesized and has high biocompatibility. Results: We observed that Mil-88a could significantly promote the expression of OA anabolism-related genes such as Col2 and also significantly inhibit the expression of OA catabolism-related genes MMP13. Besides, we observed better OARSI score in animals treated with Mil-88a nano-enzyme loading on organic metal matrix. Discussion: Overall, Mil-88a nano-enzyme could be used as a novel strategy to treat OA.

关键词： nano-enzyme osteoarthritis mouse model Mil-88a organic metal

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YTHDC2 inhibits rat bone mesenchymal stem cells osteogenic differentiation by accelerating RUNX2 mRNA degradation via m6A methylation

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HELIYON 2023年第8期9卷 e18876页

作者： Ma, Bo Cao, Pei Zhang, Lichen Zhu, Hongyi ye, xuwen Wang, Lingjun Chen, Liang Peking Univ Dept Trauma & Orthoped Peoples Hosp Beijing Peoples R China Nankai Univ Sch Med Tianjin Peoples R China Soochow Univ Affiliated Hosp 1 Dept Gen Surg Suzhou 215006 Jiangsu Peoples R China Soochow Univ Affiliated Hosp 1 Orthoped Inst Dept Orthoped Suzhou 215006 Jiangsu Peoples R China

As the most abundant internal mRNA modification, N6-methyladenosine (m6A) RNA methylation has been found to influence many biological events including bone mesenchymal stem cells (BMSCs) osteogenic differentiation. YTH N6-methyladenosine RNA binding protein C2 (YTHDC2) is an m6A reading protein with the ability to mediate the decay of combined methylated mRNA, however its role in BMSCs osteogenic differentiation remains unknown. In this study, we first found an increase of RUNX family transcription factor 2 (RUNX2) expression and a decrease of YTHDC2 expression during the process of BMSCs osteogenic dif-ferentiation. Furthermore, we transfected BMSCs with YTHDC2 interference fragment, resulting in an increased content of RUNX2 mRNA and protein inside BMSCs. Finally, through RNA Immunoprecipitation experiments, we confirmed that YTHDC2 protein can bind to RUNX2 mRNA and accelerate its decomposition. Moreover, the immunofluorescence staining also showed a negative correlation between YTHDC2 and RUNX2. In conclusion, during BMSCs osteogenic differentiation, YTHDC2 protein showed decreased expression, resulting in a higher level of RUNX2 (mRNA and protein) expression inside cells, indicating YTHDC2 as a promising molecular target for the regulation of BMSCs osteogenic differentiation.

关键词： BMSCs Osteogenic differentiation m6A YTHDC2 RUNX2

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Why they don't trust the media: The Chinese public's perception and evaluation of news media in the social media era, taking the Liu Xuezhou incident as an example

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GLOBAL MEDIA AND CHINA 2024年第4期9卷 521-541页

作者： ye, Xiaoyan Sun, xuwen Qufu Normal Univ Qufu Peoples R China Shenzhen Univ Shenzhen Peoples R China

With the advent of the post-pandemic era characterised by increasing social uncertainty, the public is placing higher demands on the trust in news media. According to the 2023 Edelman Trust Barometer, the image of the media in the public's mind is deteriorating. Why don't they trust the media? What are the reasons given by the public when the media is denounced as unscrupulous? Taking the Liu Xuezhou incident as a typical case, the study extracts key elements from 5,225 related microblog texts, identifies the main topics of public discussion with the help of LDA topic modelling, clarifies the referential dimension of unscrupulous media with the help of pointwise mutual information, and analyzes netizens' evaluations related to different referential levels of the media through visual semantic networks, which can help formulate dialectical thinking on media trust in the context of China in the era of social media.

关键词： LDA topic modelling media trust positive trust social media

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Design and Stage Analysis of a Non-Invasive Monitoring System for Determining Formaldehyde Release From Wood-Based Panels by the Chamber Method

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IEEE ACCESS 2024年 12卷 137952-137969页

作者： ye, Jinfei Lu, Yang Wang, Kuokuo Jiao, Qingchun Chen, xuwen Wang, Lijun Zhejiang Jinhui Digital Technol Co Ltd Hangzhou 310015 Zhejiang Peoples R China Zhejiang Light Ind Prod Inspect & Res Inst Hangzhou 310018 Zhejiang Peoples R China Zhejiang Univ Sci & Technol Sch Automat & Elect Engn Hangzhou 310023 Peoples R China Zhejiang Inst Standardizat Zhijiang Standardizat T Hangzhou 310063 Zhejiang Peoples R China Technol Innovat Ctr State Market Regulat Managemen Hangzhou 310063 Zhejiang Peoples R China

With the extensive application of wood-based panels in the home decor industry, determining formaldehyde release form wood-based panels (FRWBP) is crucial for ensuring environmental air quality and human health. This article addresses the issues of insufficient monitoring of the testing stages for FRWBP and the difficulty in tracing the operation records of operator during determining FRWBP by the chamber method. It proposes a system for monitoring and analyzing the testing stages of FRWBP based on electrical monitoring. The system employs non-invasive sensing technology that does not interfere with normal testing process to collect real-time electrical usage data from the devices. The data is uploaded to an industrial computer for storage and processing. Statistical features of the electrical time series data are extracted using a sliding window approach and optimized with Principal Component Analysis (PCA). Subsequently, the binary tree support vector machine algorithm (BTSVM) optimized by k-means clustering algorithm is used to identify the detection states of the wood-based panels. Experimental results show that the system can achieve traceability of the formaldehyde detection stage records of artificial boards, and the designed algorithm model can also effectively identify the detection status of wood-based panels. The system and methods proposed in this paper have significant prospects for implementation in monitoring and traceability management within the testing industry.

关键词： Environmental monitoring Temperature measurement Support vector machines Sensor phenomena and characterization Noninvasive treatment Formaldehyde Principal component analysis Air quality FRWBP chamber method non-invasive PCA k-means BTSVM

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Mechanistic insights into the selective photocatalytic degradation of dyes over TiO_(2)/ZSM-11

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Frontiers of Environmental Science & Engineering 2023年第8期17卷 127-136页

作者： Zhou Zhang Kai Huo Tingxuan Yan xuwen Liu Maocong Hu Zhenhua Yao Xuguang Liu Tao ye Department of Chemical Engineering Jianghan UniversityWuhan 430056China College of Materials Science and Engineering Qingdao University of Science and TechnologyQingdao 266042China Department of Civil and Environmental Engineering South Dakota School of Mines and TechnologyRapid CitySD 57701USA State Key Laboratory of Precision Blasting Jianghan UniversityWuhan 430056China

Photocatalytic degradation is a promising way to eliminate dye *** this work,a series of TiO2/ZSM-11(TZ)nanocomposites were prepared using a facile solid state dispersion *** orange(MO),methylene blue(MB),and rhodamine B(RhB)were intentionally chosen as target substrates in the photocatalytic degradation *** to pristine TiO2,negative effect was observed on MO degradation while promoted kinetics were collected on MB and RhB over TZ ***,a much higher photocatalytic rate was interestingly achieved on RhB than MB,which indicated that a new factor has to be included other than the widely accepted electrostatic interaction mechanism to fully understand the selective photodegradation *** characterizations showed that TiO2 and ZSM-11 physically mixed and maintained both the whole framework and local structure without chemical *** different trends observed in surface area and the photo-absorption ability of TZ composites with reaction performance further excluded both as the promotion ***,adsorption energies predicted by molecular dynamics simulations suggested that differences in the adsorption strength played a critical *** work provided a deep mechanistic understanding of the selective photocatalytic degradation of dyes reactions,which helps to rationally design highly efficient photocatalysts.

关键词： Selective dye degradation Photocatalysis TiO2 ZSM-11 Chemisorption

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Numerical simulation research on hot stretch straightening of thin-walled T-shaped profile of titanium alloy assisted by resistance heating

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INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY 2022年第9-10期121卷 6483-6495页

作者： Deng, xuwen Hui, Songxiao ye, Wenjun Liu, Rui Huang, Liang GRINM Grp Co LTD State Key Lab Nonferrous Met & Proc Beijing 100088 Peoples R China GRIMAT Engn Inst Co LTD Beijing 101407 Peoples R China Gen Res Inst Nonferrous Met Beijing 100088 Peoples R China Huazhong Univ Sci & Technol Sch Mat Sci & Engn State Key Lab Mat Proc & Die & Mould Technol Wuhan 430074 Peoples R China

In accordance with the bending deformation of titanium alloy profile generated by hot extrusion, the hot stretch straightening assisted by resistance heating is an effective strategy to improve the straightness accuracy. In order to reveal the heat transfer behavior and deformation mechanism of titanium alloy profile during hot straightening, this study focuses on Ti-6.5Al-2Zr-1Mo-1 V alloy thin-walled T-shaped profile with unequal thicknesses. The flow stress curves of profile within temperatures from 400 to 800 celcius, strain rate at 5 center dot 10(-4) s(-1) were obtained by uniaxial tensile tests. The thermal boundary conditions between profile and air were determined by using inverse analysis experiment. Then, the thermal-electrical-mechanical multi-field coupling numerical simulation model was established. The temperature distribution, stress distribution, and deformation of profile were investigated systematically. The results show that the non-uniform temperature field with low temperature in thinner wall region and high temperature in thicker wall region would form during resistance heating. The stress distribution of stretch straightening process was determined by the temperature distribution and the initial bending shape of profile jointly, thereby affecting the springback deflection and direction after unloading. The established numerical simulation models, which have been verified by process experiments, could lay foundation for the prediction and process optimization in hot stretch straightening of titanium profile.

关键词： Titanium alloy thin-walled T profile Resistance heating Hot stretch straightening Numerical simulation

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Revealing systematic changes in the transcriptome during the transition from exponential growth to stationary phase

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MSYSTEMS 2025年第1期10卷 e0131524页

作者： Lim, Hyun Gyu Gao, ye Rychel, Kevin Lamoureux, Cameron Lou, xuwen A. Palsson, Bernhard O. Inha Univ Dept Biol Sci & Bioengn Incheon South Korea Univ Calif San Diego Dept Bioengn San Diego CA 92093 USA Joint BioEnergy Inst Emeryville CA 94608 USA Shandong Univ Hosp 2 Jinan Shandong Peoples R China Tech Univ Denmark Novo Nord Fdn Ctr Biosustainabil Lyngby Denmark

The composition of bacterial transcriptomes is determined by the transcriptional regulatory network (TRN). The TRN regulates the transition from one physiological state to another. Here, we use independent component analysis to monitor the composition of the transcriptome during the transition from the exponential growth phase to the stationary phase. With Escherichia coli K-12 MG1655 as a model strain, we trigger the transition using carbon, nitrogen, and sulfur starvation. We find that (i) the transition to the stationary phase accompanies common transcriptome changes, including increased stringent responses and reduced production of cellular building blocks and energy regardless of the limiting element;(ii) condition-specific changes are strongly associated with transcriptional regulators (e.g., Crp, NtrC, CysB, Cbl) responsible for metabolizing the limiting element;and (iii) the shortage of each limiting element differentially affects the production of amino acids and extracellular polymers. This study demonstrates how the combination of genome-scale datasets and new data analytics reveals the fundamental characteristics of a key transition in the life cycle of bacteria.

关键词： stationary phase systems biology independent component analysis nutrient starvation stress transcriptome RNA-sequencing

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Efficacy and safety of coenzyme Q10 in heart failure: a meta-analysis of randomized controlled trials

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BMC CARDIOVASCULAR DISORDERS 2024年第1期24卷 592页

作者： Xu, Jiayi Xiang, Luwei Yin, xuwen Song, Haiyan Chen, Chen Yang, Bei ye, Hongfang Gu, Zejuan Nanjing Univ Chinese Med Sch Nursing 138 Xianlin Rd Nanjing 210023 Jiangsu Peoples R China Nanjing Med Univ Sch Nursing 101 Longmian Rd Nanjing 211166 Jiangsu Peoples R China Nanjing Drum Tower Hosp Dept Nursing 123 Zhongshan Rd Nanjing 210008 Jiangsu Peoples R China Nanjing Med Univ Affiliated Hosp 1 Jiangsu Prov Hosp Dept Nursing 300 Guangzhou Rd Nanjing 210029 Jiangsu Peoples R China Soochow Univ Suzhou Med Coll 199 Renai Rd Suzhou 215123 Jiangsu Peoples R China

Background The effectiveness and adverse effects of coenzyme Q10 for heart failure remain unclear owing to small sample sizes and variations in the quality of existing studies in literature. Methods The databases of EMBASE, PubMed, Web of Science, CINAHL databases, Scopus, Cochrane Central Register of Controlled Trials, VIP, Wanfang, and CNKI were searched for randomized controlled trials on the coenzyme Q10-assisted treatment of heart failure. Relevant literature was retrieved, data were extracted, and the risk of bias of the included studies was evaluated by two investigators independently using the Review Manager 5.4 software and the STATA 15 software. Results In total, 33 studies were included in this meta-analysis, which showed that all-cause mortality [RR = 0.64, 95% CI (0.48, 0.85), P = 0.002;GRADE: moderate quality], hospitalization for heart failure [RR = 0.50, 95% CI (0.37, 0.67), P < 0.00001;GRADE: moderate quality], New York Heart Association classification [MD = - 0.29, 95% CI (- 0.39, - 0.19), P < 0.00001;GRADE: low quality], and brain natriuretic peptide level [MD = - 91.97, 95% CI (- 103.11, - 80.83), P < 0.00001;GRADE: low quality] were lower in the coenzyme Q10 group than in the control group. Meanwhile, left ventricular ejection fraction [MD = 0.51, 95% CI (0.31, 0.71), P < 0.00001;GRADE: low quality] and 6-min walk test result [MD = 31.70, 95% CI (19.96, 43.43), P < 0.00001;GRADE: moderate quality] were better than those in the control group. Conclusions According to the existing evidence, coenzyme Q10 reduces all-cause mortality, hospitalization for heart failure, New York Heart Association classification, and brain natriuretic peptide level and improves left ventricular ejection fraction and 6-min walk test result in those with heart failure without major adverse effects. Trial registration This study protocol was registered in the International Prospective Register of Systematic Reviews (PROSPERO, http://***/prospero), with the

关键词： Heart failure Safety outcomes Coenzyme Q10 Meta analysis Randomized controlled trial

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Nomogram to screen older adult patients attending the radiology department for sarcopenia

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BMC GERIATRICS 2025年第1期25卷 1-9页

作者： Yuan, Guiying ye, Guoxi Hu, Jianguang Hu, Huimin Shi, Chanmei Zhang, ye Huang, Junbing Li, Zhiqiong Zeng, xuwen Tan, Rongshao Xiong, Yuchao Jinan Univ Guangzhou Red Cross Hosp Dept Resp Guangzhou Peoples R China Jinan Univ Guangzhou Red Cross Hosp Dept Radiol 396 Tongfu Rd Guangzhou Guangzhou 510220 Guangdong Provi Peoples R China Jinan Univ Guangzhou Red Cross Hosp Dept Nephrol Guangzhou Peoples R China Jinan Univ Guangzhou Red Cross Hosp Inst Dis Oriented Nutr Res Dept Clin Nutr Guangzhou Peoples R China

BackgroundSarcopenia is an age-related syndrome that can impact the physical and mental health of older adults. However, it is often overlooked in clinical practice. Therefore, we aim to construct a nomogram based on simplified discriminant parameters for screening older adult patients for sarcopenia *** cross-sectional study included 654 patients aged >= 60 years who underwent an examination in the radiology department between October 2023 and June 2024. Patients were diagnosed with sarcopenia according to the method and cutoff value criteria proposed the Asian Working Group on Sarcopenia (AWGS) 2019 criteria. Calf circumference (CC), SARC-F score, mid-upper arm circumference (MUAC), and SARC-CalF score were used as simplified discriminant parameters for sarcopenia. The discriminative ability of these parameters for sarcopenia was assessed using receiver operating characteristic analysis. Additionally, we included each screening parameter and evaluated it's important for screening for the presence of sarcopenia via univariate and multivariate logistic regression analysis to develop a new screening nomogram model. The performance of the nomogram was evaluated using receiver operating characteristic curves, and the performance of the nomogram model was compared to that of CC, SARC-F, MUAC, and the SARC-CalF using the Delong *** the 654 subjects, 120 (18.3%) were diagnosed with sarcopenia, and the areas under the curve (AUCs) of the CC, SARC-F, MUAC, and SARC-CalF were 0.73, 0.61, 0.66, and 0.70, respectively. The multivariate analysis results revealed that older age, male sex, low CC, low MUAC, and low strength were related to sarcopenia. A nomogram model constructed with these five variables had an AUC of 0.84. The DeLong test showed that the diagnostic efficacy of the joint model was significantly higher than that of CC, SARC-F, MUAC, and *** simple nomogram based on simplified discriminant parameters offers personalized

关键词： Sarcopenia Nomogram Calf circumference SARC-F SARC-CalF

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