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Purines enrich root-associated Pseudomonas and improve wild soybean growth under salt stress

NATURE COMMUNICATIONS 2024年第1期15卷 3520页

作者： Zheng, Yanfen cao, xuwen Zhou, Yanan Ma, Siqi Wang, Youqiang Li, Zhe Zhao, Donglin Yang, Yanzhe Zhang, Han Meng, Chen Xie, Zhihong Sui, Xiaona Xu, Kangwen Li, Yiqiang Zhang, Cheng-Sheng Chinese Acad Agr Sci Tobacco Res Inst Marine Agr Res Ctr Qingdao 266101 Peoples R China Shandong Univ Inst Marine Sci & Technol Qingdao 266200 Peoples R China Shandong Agr Univ Coll Resources & Environm Natl Engn Lab Efficient Utilizat Soil & Fertilizer Tai An 271018 Peoples R China

The root-associated microbiota plays an important role in the response to environmental stress. However, the underlying mechanisms controlling the interaction between salt-stressed plants and microbiota are poorly understood. Here, by focusing on a salt-tolerant plant wild soybean (Glycine soja), we demonstrate that highly conserved microbes dominated by Pseudomonas are enriched in the root and rhizosphere microbiota of salt-stressed plant. Two corresponding Pseudomonas isolates are confirmed to enhance the salt tolerance of wild soybean. Shotgun metagenomic and metatranscriptomic sequencing reveal that motility-associated genes, mainly chemotaxis and flagellar assembly, are significantly enriched and expressed in salt-treated samples. We further find that roots of salt stressed plants secreted purines, especially xanthine, which induce motility of the Pseudomonas isolates. Moreover, exogenous application for xanthine to non-stressed plants results in Pseudomonas enrichment, reproducing the microbiota shift in salt-stressed root. Finally, Pseudomonas mutant analysis shows that the motility related gene cheW is required for chemotaxis toward xanthine and for enhancing plant salt tolerance. Our study proposes that wild soybean recruits beneficial Pseudomonas species by exudating key metabolites (i.e., purine) against salt stress.

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Transcriptome analysis of blood for the discovery of sex-related genes in ricefield eelMonopterus albus

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FISH PHYSIOLOGY AND BIOCHEMISTRY 2020年第4期46卷 1507-1518页

作者： Ding, Weidong cao, Liping cao, Zheming Bing, xuwen Chinese Acad Fishery Sci Key Lab Freshwater Fisheries & Germplasm Resource Minist Agr Freshwater Fisheries Res Ctr Wuxi 214081 Jiangsu Peoples R China

The blood acts as a transfer channel for a variety of factors in the whole body. The ricefield eel(Monopterus albus) is a protogynous hermaphrodite vertebrate. Until now, no research has reported an analysis of the blood transcriptome during the process of sexual development in the ricefield eel. In this study, the transcriptome sequencing of blood samples from male and female ricefield eels was completed with a total of 34.70 Gb clean data. The clean data of each sample all reached 5.23 GB, and the percent of the Q30 basic group was 88.62% and above. A total of 106,369 unigenes were obtained after assembly, including 13,296 unigenes with a length of more than 1 kb. Further functional annotation analysis showed that there are 28,522 unigenes that can be annotated. The annotations of genes with differential expression revealed that there were 563 genes with significant differential expression in the blood of male and female ricefield eels, including 91 upregulated genes and 472 downregulated genes. Among which, 14 genes may be closely related to sex differentiation, the qPCR was used to confirmed the expression pattern of those genes and result shown that 11 genes were downregulated and 3 genes were upregulated, consistent with the results of our RNA-Seq analysis. This blood transcript dataset will open future research avenues on ricefield eel sex development and differentiation.

关键词： Blood Transcriptome Sex development genes Ricefield eel

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Transcriptomic responses of the liver of mandarin fish (Siniperca chuatsi) under hypoxic stress

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JOURNAL OF FISH BIOLOGY 2023年第1期103卷 44-58页

作者： Ding, Weidong cao, Liping cao, Zheming Bing, xuwen Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Key Lab Freshwater Fisheries & Germplasm Resources Minist Agr Wuxi 214081 Peoples R China

Environmental hypoxia is becoming more prevalent in aquatic environments of mandarin fish (Siniperca chuatsi) aquaculture because of eutrophication and climate change. Little information is available on the molecular mechanisms of the detrimental effects of hypoxia in this species. In this study, the authors assembled a transcriptome for mandarin fish exposed to lower oxygen conditions at different times (24 and 96 h). The antioxidant enzymatic activities of catalase, glutathione, superoxide dismutase, glutamic pyruvic transaminase and malondialdehyde significantly increased at 6 or 12 h but decreased after reaching a climax during 96 h hypoxia stress. The gene ontology study revealed 27,616 transcripts, whereas the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed 25 linked pathways. Significant changes in the expression of certain genes involved in protein processing in the endoplasmic reticulum, the calcium signalling system and inositol phosphate metabolism were discovered using the KEGG pathway analysis. In the liver, 97 genes were differentially expressed between the control and experimental groups. The expression level of 28 differentially expressed genes (DEGs) under different hypoxic stress conditions was detected using real-time PCR and compared to transcriptome sequencing results. The result showed that some genes in the experimental group associated with hypoxic stress, such as hif, ho-1a, ho-1b, igfbp1, hsp90 alpha and hsp90 beta, were significantly upregulated compared with those in the control group. The large amount of transcriptome data from this research has enlarged the mandarin fish gene and genome bioinformation. The identified DEGs and pathways are useful in further studies of biological responses to hypoxia.

关键词： differentially expressed genes hypoxia mandarin fish transcriptome

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Natural passivation behavior and its influence on chloride-induced corrosion resistance of stainless steel in simulated concrete pore solution

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JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T 2020年第6期9卷 12378-12390页

作者： Yuan, xuwen Wang, Xuan cao, Yang Yang, Huaiyu Chinese Acad Sci Inst Met Res Shenyang 110016 Peoples R China Univ Sci & Technol China Sch Mat Sci & Engn Hefei 230026 Peoples R China

This study presents a combined electrochemical and X-ray photoelectron spectroscopy (XPS) surface analytical investigation on the natural passivation process and its effect on Cl- induced corrosion resistance of traditional carbon steel, ferritic and austenitic stainless steels (SSs) (i.e. HRB400 steel, 430 and 304 SSs) in simulated concrete pore solution (SPS). It was found that a complete and more compact passive film with a stratified structure could be naturally formed on the tested steel surfaces after different immersion time. The chemical composition of the passive film and its corrosion resistance strongly depended on the alloying element and natural passivation time. For HRB400 steel, the outer layer of natural passive film was mainly composed of Fe3+ species and the inner layer was predominated by Fe2+ ones, while the outer layer of natural passive film formed on SSs surface consisted of Fe and Cr oxides and hydroxides, enriched in Fe3+ species, and an inner layer was made of mixed Fe2+ and Cr3+ oxides (enriched in Fe2+ species). The natural pre-passivation could effectively improve the Cl- induced corrosion resistance of three steels. Compared with HRB400 steel, both austenitic and ferritic SSs exhibited the better corrosion resistance due to the formation of Cr-enriched passive film, of which, 304 SS had the highest pitting corrosion resistance. (C) 2020 The Author(s). Published by Elsevier B.V.

关键词： Stainless steel Natural passivation Pitting corrosion EIS Cyclic potentiodynamic polarization

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CELF(CUG-BP,Elav-like family)家族研究进展

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鲁东大学学报（自然科学版） 2017年第3期33卷 231-237页

作者：曹绪文刘学敏冯铄淇杨青云李红岩中国海洋大学海洋生命学院海洋生物多样性与进化研究所山东青岛266003

CELF(CUG-BP,Elav-like family)家族作为RNA结合蛋白广泛存在于动物和植物中,其在细胞核和胞质内调控多个转录后RNA加工过程,包括前体mRNA的选择性剪接、mRNA降解和翻译等.CELF家族在人和其他哺乳动物中有6个成员,分别是:CELF1~6,根据... 详细信息

CELF(CUG-BP,Elav-like family)家族作为RNA结合蛋白广泛存在于动物和植物中,其在细胞核和胞质内调控多个转录后RNA加工过程,包括前体mRNA的选择性剪接、mRNA降解和翻译等.CELF家族在人和其他哺乳动物中有6个成员,分别是:CELF1~6,根据进化关系可分为两个亚家族,CELF1~2和CELF3~6亚家族.CELF介导的转录后调控对早期胚胎发育、配子形成以及肌肉和神经系统功能都很重要.此外,CELF还与强直性肌营养不良、免疫细胞功能、癌症发生等密切相关.本文对CELF家族成员的生物学功能以及CELF对人类疾病影响进行了综述.

关键词： CELF蛋白 RNA结合蛋白强直性肌营养不良癌症

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Identification and characterization of a LTR retrotransposon from the genome of Cyprinus carpio var. Jian

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GENETICA 2016年第3期144卷 325-333页

作者： cao, Liping Yin, Guojun cao, Zheming Bing, xuwen Ding, Weidong Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Minist Agr Key Lab Freshwater Fisheries & Germplasm Resource Wuxi 214081 Peoples R China

A Ty3/gypsy-retrotransposon-type transposon was found in the genome of the Jian carp (Cyprinus carpio var. Jian) in a previous study (unpublished), and was designated a JRE retrotransposon (Jian retrotransposon). The full-length JRE retrotransposon is 5126 bp, which includes two long terminal repeats of 470 bp at the 5' end and 453 bp at the 3' end, and two open reading frames between them: 4203 bp encoding the group-specific antigen (GAG) and polyprotein (POL). The pol gene has a typical Ty3/gypsy retrotransposon structure, and the gene order is protease, reverse transcriptase, RNase H, and integrase (PR-RT-RH-IN). A phylogenetic analysis of the pol gene showed that it has similarities of 40.7, 40, and 32.8 %, to retrotransposons of Azumapecten farreri, Mizuhopecten yessoensis, and Xiphophorus maculatus, respectively. Therefore, JRE might belong to the JULE retrotransposon family. The copy number of the JRE transposon in the genome of the Jian carp is 124, determined with real-time quantitative PCR. The mRNA of the JRE retrotransposon is expressed in five Jian carp tissues, the liver, kidney, blood, muscle, and gonad, and slightly higher in the kidney and liver than in the other tissues.

关键词： Cyprinus carpio var. Jian Retrotransposon Copy number Fluorescent in situ hybridization

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Insight into Iron Leaching from an Ascorbate-Oxidase-like Fe−N−C Nanozyme and Oxygen Reduction Selectivity**

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Angewandte Chemie 2023年第27期135卷

作者： xuwen cao Caixia Zhu Qing Hong Xinghua Chen Kaiyuan Wang Prof. Yanfei Shen Prof. Songqin Liu Prof. Yuanjian Zhang Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device Jiangsu Province Hi-Tech Key Laboratory for Bio-Medical Research School of Chemistry and Chemical Engineering Southeast University Nanjing 211189 China Medical School Southeast University Nanjing 210009 China

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Study of university physics teaching based on information technology with education practice

Study of university physics teaching based on information te...

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作者： cao, Yonghua Gao, Yinhao Guo, xuwen Zhang, Lianfeng Mechanical and Electrical Department Henan Institute of Science Technology University Xinxiang Henan China Economics and Management Department Henan Institute of Science Technology University Xinxiang Henan China

ISBN: (纸本)9783642237522

Today's university physics teaching is facing some difficulties such as short teaching hours and richer contents. This article firstly analyses the present situation of university physics. Then it points out that the way out of the university physics teaching reform is the organic integration of information technology and university physics teaching. The additional role of information technology would be gave full play and then the teaching methods can be optimized. According to teaching practice, some suggestions about integrated approach are proposed in this article. © 2011 Springer-Verlag Berlin Heidelberg.

关键词： Computers

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Multidisciplinary optimization of electromagnetic linear actuators for direct-drive systems with a combination of static and dynamic performance

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COMPEL-THE INTERNATIONAL JOURNAL FOR COMPUTATION AND MATHEMATICS IN ELECTRICAL AND ELECTRONIC ENGINEERING 2023年第2期42卷 476-493页

作者： Chi, xuwen Tan, cao Li, Bo Lu, Jiayu Gu, Chaofan Fu, Changzhong Shandong Univ Technol Sch Transportat & Vehide Engn Zibo Peoples R China Shandong Zhongbaokang Med Equipment Co Zibo Peoples R China

Purpose The purpose of this paper is to solve the common problems that traditional optimization methods cannot fully improve the performance of electromagnetic linear actuators (EMLAs). Design/methodology/approach In this paper, a multidisciplinary optimization (MDO) method based on the non-dominated sorting genetic algorithm-II (NSGA-II) algorithm was proposed. An electromagnetic-mechanical coupled actuator analysis model of EMLAs was established, and the coupling relationship between static/dynamic performance of the actuator was analyzed. Suitable optimization variables were designed based on fuzzy grayscale theory to address the incompleteness of the actuator data and the uncertainty of the coupling relationship. A multiobjective genetic algorithm was used to obtain the optimal solution set of Pareto with the maximum electromagnetic force, electromagnetic force fluctuation rate, time constant and efficiency as the optimization objectives, the final optimization results were then obtained through a multicriteria decision-making method. Findings The experimental results show that the maximum electromagnetic force, electromagnetic force fluctuation rate, time constants and efficiency are improved by 18.1%, 38.5%, 8.5% and 12%, respectively. Compared with single-discipline optimization, the effectiveness of the multidiscipline optimization method was verified. Originality/value This paper proposes a MDO method for EMLAs that takes into account static/dynamic performance, the proposed method is also applicable to the design and analysis of various electromagnetic actuators.

关键词： Electromagnetic linear actuator Fuzzy grayscale theory Multidisciplinary optimization Performance analysis Multicriteria decision-making Mechatronics Actuators Power losses Pareto optimization Coupled systems

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Study of Wall Strain during LaNi5Alloy's Hydrogen Absorption/Desorption Process

Study of Wall Strain during LaNi5Alloy's Hydrogen Absorption...

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2022 International Conference on Energy and Power Engineering, EPE 2022

作者： Yin, xuwen cao, Shuaijie Yang, Fusheng Imin, Rahmatjan School of Mechanical Engineering Xinjiang University Xinjiang Urumqi830017 China School of Chemical Engineering and Technology Xi'an Jiaotong University Shanxi Xi'an710049 China School of Mathematics and Systems Science Xinjiang University Xinjiang Urumqi830017 China

An experimental LaNi5 alloy's hydrogen absorption/desorption system was established to explore the strain generation process mechanism on the reactor wall during the cyclic hydrogen absorption/desorption process of LaNi5 alloy powders at room temperature after different cycles and periods. On this basis, the influence of the hydrogen pressure on the wall strain of the reactor was verified. Results showed that with the increase in cycles, the reactor wall was subjected to an expansion strain, and the closer to the reactor bottom, the greater the strain value. As the hydrogen pressure grew from 3.0 to 4.0 MPa, the strain value at the reactor bottom increased obviously. Still, it did not grow during cycling experiments with hydrogen, indicating that the agglomeration phenomenon was mainly attributed to the pulverization effect after hydrogen absorption by the alloy instead of the gas pressure itself. After 24 cyclic reactions, the wall strain at the 1/5 position from the bottom experienced changes in growth laws since the thermal strain was not enough to influence the expansion strain triggered by agglomeration, and the closer to the reactor top, the more greatly the wall strain was influenced by reaction heat. © Published under licence by IOP Publishing Ltd.

关键词： Binary alloys

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