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Microenvironment-tailored micelles restrain carcinoma-astrocyte crosstalk for brain metastasis

JOURNAL OF CONTROLLED RELEASE 2022年 349卷 520-532页

作者： Zhao, Zhenhao Zhang, Yujie Li, Chao Li, xuwen Chu, Yongchao Guo, Qin Zhang, Yiwen Xia, Weiyi Liu, Peixin Chen, Hongyi Wang, Yu Li, Chufeng Sun, Tao jiang, Chen Fudan Univ Sch Pharm Dept Pharmaceut Key Lab Smart Drug Delivery Shanghai 201203 Peoples R China Fudan Univ MOE Frontiers Ctr Brain Sci Shanghai 201203 Peoples R China

Breast-to-brain metastatic cells can interact with the surrounding cells, including astrocytes and microglia, to generate a pro-tumorigenic niche. Breast-to-brain metastasis can be treated using a dual strategy of eliminating metastatic tumor cells and normalizing their localized microenvironment. The effective accumulation of drugs at the action site of metastasis is crucial to realizing the above strategy, especially when dealing with the bloodbrain barrier (BBB)-penetrating and tumor-targeting tactics. Here, we establish an in-situ microenvironmenttailored micelle (T-M/siRNA) to co-deliver therapeutic siRNA and paclitaxel (PTX) into the breast-to-brain metastasis. Anchored with a D-type cyclic peptide, T-M/siRNA can penetrate the BBB and subsequently target the brain metastases. Upon internalization by metastatic tumor cells, T-M/siRNA can release PTX in the highlevel glutathione (GSH), resulting in killing cancer cells. Meanwhile, the micellar structure is dissociated, resulting in lowering the charge density to release the loaded siRNA that can targeted downregulate the expression of protocadherin 7 (PCDH7). Treatment of model mice revealed that T-M/siRNA can inhibit the abnormal activation of astrocytes and immunosuppressive activation of microglia, resulting in significantly enhanced synergistic anti-tumor efficacy. This study indicates that the micelle system can serve as a hopeful strategy to treat breast-to-brain metastasis.

关键词： Breast -to -brain metastasis Micelle Microenvironment Protocadherin 7 Paclitaxel

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Interparticle Charge-Transport-Enhanced Electrochemiluminescence of Quantum-Dot Aerogels

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ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2023年第2期62卷 e202214487-e202214487页

作者： Gao, xuwen jiang, Guocan Gao, Cunyuan Prudnikau, Anatol Huebner, Rene Zhan, Jinhua Zou, Guizheng Eychmueller, Alexander Cai, Bin Shandong Univ Sch Chem & Chem Engn Jinan 250100 Peoples R China Tech Univ Dresden Phys Chem D-01069 Dresden Germany Helmholtz Zentrum Dresden Rossendorf Inst Ion Beam Phys & Mat Res D-01328 Dresden Germany

Electrochemiluminescence (ECL) represents a widely explored technique to generate light, in which the emission intensity relies critically on the charge-transfer reactions between electrogenerated radicals. Two types of charge-transfer mechanisms have been postulated for ECL generation, but the manipulation and effective probing of these routes remain a fundamental challenge. Here, we demonstrate the design of quantum dot (QD) aerogels as novel ECL luminophores via a versatile water-induced gelation strategy. The strong electronic coupling between adjacent QDs enables efficient charge transport within the aerogel network, leading to the generation of highly efficient ECL based on the selectively improved interparticle charge-transfer route. This mechanism is further verified by designing CdSe-CdTe mixed QD aerogels, where the two mechanistic routes are clearly decoupled for ECL generation. We anticipate our work will advance the fundamental understanding of ECL and prove useful for designing next-generation QD-based devices.

关键词： Charge Transfer Electrochemiluminescence Electrochemistry Quantum Dots Sol-Gel Processes

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BMP亚家族成员中影响绵羊繁殖性能的基因研究进展

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家畜生态学报 2024年第7期45卷 1-8页

作者：刘梦兰邵旭文单雪松姜怀志吉林农业大学动物科学技术学院长春130118 农业农村部畜禽资源(羊)评价利用重点实验室长春130118

排卵数和产羔数是反映绵羊繁殖性状的关键性指标,也是绵羊重要的经济性状,二者属于中低遗传力性状,受基因型、环境和营养状况等多重因素共同影响。由BMP、GDF和AMH等组成的BMP亚家族是TGF-β超家族的重要组成部分,BMPR1B、BMP15和GDF9... 详细信息

排卵数和产羔数是反映绵羊繁殖性状的关键性指标,也是绵羊重要的经济性状,二者属于中低遗传力性状,受基因型、环境和营养状况等多重因素共同影响。由BMP、GDF和AMH等组成的BMP亚家族是TGF-β超家族的重要组成部分,BMPR1B、BMP15和GDF9是目前与绵羊繁殖性能相关性研究最为深入的基因。该文对BMP亚家族成员中提升绵羊排卵数及产羔数的基因的SNP位点进行归纳,总结了这些基因影响繁殖性能的SNP位点的发现,品种、定位、表型,以及对排卵数和产羔率的影响机制,旨在为绵羊多胎品种的选育和高效繁殖提供理论参考。

关键词：突变位点繁殖性状 BMPs 主效基因绵羊

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Screening and Expression Analysis of POD Gene in POD-H2O2 Pathway on Bud Dormancy of Pear (Pyrus pyrifolia)

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FORESTS 2024年第3期15卷 434页

作者： Xiang, xuwen Song, Keke Li, Yinyin Zhang, Chenyu Zhou, Ruiqi Feng, Yu You, Jingnan Wu, Jingdong Zhang, Yanhui jiang, Cuicui Li, Yongyu Fujian Agr & Forestry Univ Coll Hort Key Lab Minist Educ Genet Breeding & Multiple Utilizat Cro Fuzhou 350002 Peoples R China Fujian Agr & Forestry Univ Inst Nat Prod Hort Plants Fuzhou 350002 Peoples R China Agr & Rural Bur Jianning Cty Econ Crop Stn Sanming 354500 Peoples R China Agr & Rural Bur Yongtai Cty Econ Crop Stn Fuzhou 350700 Peoples R China Fujian Acad Agr Sci Fruit Res Inst Fuzhou 350002 Peoples R China

Bud endodormancy represents a pivotal and intricate biological process influenced by both genetic and epigenetic factors, the exact mechanism of which remains elusive. Hydrogen peroxide (H2O2) functions as a signalling molecule in the regulation of dormancy, with peroxidase (POD) playing a crucial role in governing H2O2 levels. Our prior transcriptomic and metabolomic investigations into diverse pear dormancy phases posited that POD predominantly oversees pear bud dormancy. In this study, we utilised qRT-PCR to screen the most significantly expressed gene, Pyrus pyrifolia POD4-like (PpPOD4-like), from seven POD genes. Subsequently, H2O2 test kits, overexpression methods, and subcellular localisation techniques were employed to assess changes in H2O2 content, POD activity, PpPOD4-like expression, and its cellular positioning during pear bud dormancy. Subcellular localisation experiments revealed that PpPOD4-like is situated on the cell membranes. Notably, H2O2 content exhibited a rapid increase during endodormancy and decreased swiftly after ecodormancy. The fluctuation pattern of POD activity aligned with that of H2O2 content. Additionally, PpPOD4-like expression was markedly upregulated, displaying an overall upward trajectory. Our findings indicate that PpPOD4-like modulates H2O2 levels by regulating POD activity, thereby actively participating in the intricate regulation of pear dormancy processes.

关键词： endodormancy overexpression H2O2 POD genes

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Recent advances in the synthesis of biodegradable polyesters by sustainable polymerization: lipase-catalyzed polymerization

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RSC ADVANCES 2020年第59期10卷 36230-36240页

作者： Liu, Ying Song, Lijie Feng, Na jiang, Wei Jin, Yongri Li, xuwen Jilin Univ Coll Chem 2699 Qianjin Rd Changchun 130012 Jilin Peoples R China Jilin Prov Acad Tradit Chinese Med Clin Hosp 1 Changchun 130021 Peoples R China Zhengzhou Univ Acad Med Sci Dept Mol Pathol Applicat Ctr Precis MedAffiliated Hosp 2 Zhengzhou 450052 Henan Peoples R China

Over the past three decades, enzymatic polymerization has dramatically developed and gradually broadened as a creative methodology in the construction of polymeric materials with tailor-made structures and properties. Compared with transition metal catalyst polymerizations, enzymatic polymerization is more attractive in the biomedicine field due to the metal-free residue, good biocompatibility, and few by-products. Meanwhile, enzymatic polymerization has far more activity towards macrolides. In this review, the synthesis of lipase-catalyzed polymer materials is systematically summarized, focusing on the synthesis of the complex and well-defined polymers. The enzymatic polyester synthesis was then discussed concerning the different reaction types, including ring-opening polymerization, polycondensation, a combination of ring-opening polymerization with polycondensation, and chemoenzymatic polymerization. Besides, exploration of novel biocatalysts and reaction media was also described, with particular emphasis on the enzymes obtainedviaimmobilization or protein engineering strategies, green solvents, and reactors. Finally, recent developments in catalytic kinetics and mechanistic studies through the use of spectroscopy, mathematics, and computer techniques have been introduced. Besides, we addressed the remaining central issues in enzymatic polymerization and discussed current studies aimed at providing answers.

关键词： Ring opening polymerization

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Research on the Application of BIM Technology in Construction Cost Management

Research on the Application of BIM Technology in Constructio...

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作者： Zhi Xu Xingqu Gao Zhongquan Song xuwen Tian Xiaohan jiang Harbin Far Eastinstitute of Technology Northeast Forestry University Engineering Consulting and Design Institute

In the traditional project cost management, due to the influence of many factors, its calculation accuracy is not high enough, which brings great difficulties to the construction project cost management. Using BIM technology to build a cost management model can provide very intuitive analysis results for cost managers, thus providing strong technical support for cost management of construction projects. This paper analyzes the application of BIM technology in construction cost management.

关键词： Construction engineering Cost management Technical BIM

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Fe-Mn-Cu-Ce/Al2O3 as an efficient catalyst for catalytic ozonation of bio-treated coking wastewater: Characteristics, efficiency, and mechanism

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ARABIAN JOURNAL OF CHEMISTRY 2023年第1期16卷

作者： Yuan, Run Qin, Yihe He, Can Wang, Zichen Bai, Lu Zhao, Hong jiang, Zijian Meng, Lingyao He, xuwen China Univ Min & Technol Beijing Dept Chem & Environm Engn Beijing 100083 Peoples R China Beijing Acad Sci & Technol Inst Resources & Environm Beijing 100089 Peoples R China

It is important to develop a catalyst that has high catalytic activity and can improve the degradation efficiency of refractory organic pollutants in the catalytic ozonation process. In this study, Fe-Mn-Cu-Ce/Al2O3 was synthesised via impregnation calcination for catalytic ozonation of bio-treated coking wastewater. The physical and chemical characteristics of the catalysts were analysed using X-ray diffraction (XRD), X-ray fluorescence spectrometry (XRF), X-ray photoelec-tron spectroscopy (XPS), scanning electron microscopy (SEM), and Brunauer-Emmett-Teller nitrogen adsorption-desorption methods. The effects of catalyst dosage, pH, and reflux ratio on the degradation efficiency of wastewater were examined in laboratory-scale experiments. The chem-ical oxygen demand (COD) removal rate of bio-treated coking wastewater was estimated to be 52.76 % under optimal conditions. The experiments on the catalytic mechanism demonstrated that the surface hydroxyl formed by the Lewis acid sites on the surface of the catalyst can react with ozone as the active site forming the active oxygen (center dot OH, .O2-, and 1O2), thereby efficiently degrading the organic pollutants in coking wastewater. Furthermore, a pilot-scale experiment on the catalytic ozonation of bio-treated coking wastewater was carried out using an Fe-Mn-Cu-Ce/Al2O3 catalyst, while the effects of the initial pollutant concentration, ozone concentration, and gas flow on the COD removal rate were studied on a pilot scale. It was found that the COD removal rate of the wastewater was ti 60 % under optimal parameters. After the treatment, the wastewater steadily reached the coking wastewater discharge standard (COD < 80 mg/L), while the operating cost of catalytic ozonation reached ti 0.032$/m3, thereby paving the way toward economic engineering applications. The COD degradation kinetics in the bio-treated coking wastewater followed pseudo -second-order kinetics. Three-dimensional fluorescence and gas chromatography-

关键词： Fe-Mn-Cu-Ce Catalytic ozonation Coking wastewater Catalytic mechanism Pilot-scale study

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Molecular and epidemiological characterization of Staphylococcus aureus causing bovine mastitis in China

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MICROBIAL PATHOGENESIS 2024年 191卷 106640页

作者： Zhu, Lingling Lai, Yuxin Li, xuwen Ma, Hui Gong, Fengju Sun, Xueqiang Cao, Aiqiao jiang, Tao Han, Yintao Pan, Zihao Nanjing Agr Univ Coll Vet Med OIE Reference Lab Swine Streptococcosis Nanjing Peoples R China Nanjing Agr Univ Coll Vet Med Nanjing Peoples R China China Anim Hlth & Epidemiol Ctr Qingdao Peoples R China Shenzhen Ctr Anim Dis Control & Prevent Shenzhen Peoples R China Nanjing Univ Jinling Hosp Affiliated Hosp Med Sch Dept Stomatol Nanjing Peoples R China

Background: Staphylococcus aureus is one of the most prevalent pathogens in bovine mastitis, which leads to substantial financial losses for the dairy industry. Results: In this study, S. aureus (n = 72) was isolated from 18 dairy farms in 15 provinces across China in 2021. The identification of these isolates at the species level was achieved by employing 16S rRNA sequencing. An isothermal amplification method for auxiliary detection of S. aureus was established, which can be employed not only for laboratory detection but also for point-of-care testing (POCT). Molecular characteristics of S. aureus mastitis in Chinese dairy cows were determined through MLST and spa typing. Finally, methicillin-resistant Staphylococcus aureus (MRSA) and MRSA resistance genes were detected using MIC and PCR amplification techniques. 72 isolates were identified as 12 sequence types (STs) and 7 clonal complexes (CC). ST1/CC1 was the dominant prevalent accounting for 33.3 % of the total, and exhibiting a wide distribution range. In terms of spa types, t114 was the dominant type, accounting for 31.9 % of the total, followed by t529 as the second major type. Four S. aureus strains were classified as MRSA according to their levels of oxacillin resistance (MIC >= 4 mu g/mL). Among these four MRSA strains, one of them was found to be mecA positive. However, the presence of drugresistance genes mecA and mecC was not detected in the remaining three MRSA strains, indicating the possible existence of new resistance genes. Conclusions: Our study investigated the prevalence of S. aureus mastitis in dairy cows in China, while also examined the molecular characteristics and MRSA strains. This information will help with the clinical monitoring, prevention, and control of S. aureus mastitis in dairy cattle.

关键词： Staphylococcus aureus Molecular characterization MRSA MLST Spa

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Research on virtual power plant's participation in power market under the background of energy internet in China 5

Research on virtual power plant's participation in power mar...

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2020 5th International Conference on Renewable Energy and Environmental Protection, ICREEP 2020

作者： Su, Yang Kening, Chen Yang, Wang Yu, jiang Yu, Ding xuwen, Liu Qiuyang, Ma State Grid Energy Research Institute Beijing102209 China State Grid JiangSu Electric Power Co. Ltd. Jiang-Su210008 China

Under the background of energy internet, a large influx of market entities participates in the interaction of "source network and load storage" and becomes an important resource for system regulation through the electricity marketization. Among them, the virtual power plant involves several market participators such as the distributed generation, demand side adjustable resources, distributed energy storage. The research of the operation mechanism and market-oriented trading organization for the virtual power plant becomes a popular topic in recent years. This paper proposes a novel mechanism for virtual power plants to participate in the electricity trading market, including the trading categories and auxiliary service which can be provided by the virtual power plant. © Content from this work may be used under the terms of the Creative Commons Attribution 3.0 Licence.

关键词： Virtual Power Plants

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Gradient tumor microenvironment-promoted penetrating micelles for hypoxia relief and immunosuppression reversion in pancreatic cancer treatment

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ACTA BIOMATERIALIA 2023年第1期167卷 387-400页

作者： Luo, Yifan Li, Chao Zhang, Yiwen Liu, Peixin Chen, Hongyi Zhao, Zhenhao Wang, Yu Zhou, Zheng Song, Haolin Su, Boyu Li, Chufeng Li, xuwen Zhang, Tongyu You, Haoyu Wu, Yuxing Tian, Zonghua Zhang, Shilin Guo, Yun Fan, Hongrui Chen, Qinjun jiang, Chen Sun, Tao Fudan Univ Minhang Hosp Sch Pharm Dept Pharmaceut Shanghai 201203 Peoples R China Fudan Univ MOE Frontiers Ctr Brain Sci Shanghai 201203 Peoples R China

The tumor microenvironment of pancreatic ductal adenocarcinoma (PDAC) is the main block for the penetration of chemotherapy. In the tumor microenvironment, a dense matrix composed of fibrin is formed on the exterior, while the interior is featured by high reduction, hypoxia and low pH. How to match the special microenvironment to on-demand drug release is the key to improve chemotherapeutic efficacy. Herein, a microenvironment-responsive micellar system is developed to deepen tumoral penetration. Briefly, the conjugation of a fibrin-targeting peptide to PEG-poly amino acid has been utilized to achieve accumulation of micelles in the tumor stroma. By modification of micelles with hypoxia-reducible nitroimidazole which becomes protonated under acidic conditions, their surface charge is more positive, facilitating deeper penetration into tumors. Paclitaxel was loaded onto the micelles via a disulfide bond to enable glutathione (GSH)-responsive release. Therefore, the immunosuppressive microenvironment is relived through the alleviation of hypoxia and depletion of GSH. Hopefully, this work could establish paradigms by designing sophisticated drug-delivery systems to tactfully employ and retroact the tamed tumoral microenvironment to improve the therapeutic efficacy based on understanding the multiple hallmarks and learning the mutual regulation.

关键词： Pancreatic cancer Penetration Nitroimidazole Tumor hypoxia Gemcitabine

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