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NT-seq: a chemical-based sequencing method for genomic methylome profiling

GENOME BIOLOGY 2022年第1期23卷 1页

作者： li, xuwen Guo, Shiyuan Cui, Yan Zhang, Zijian Luo, Xinlong Angelova, Margarita T. Landweber, Laura F. Wang, Yinsheng Wu, Tao P. Baylor Coll Med Dept Mol & Human Genet Houston TX 77030 USA Univ Calif Riverside Genet Genom & Bioinformat Grad Program Riverside CA 92521 USA Columbia Univ Dept Biochem & Mol Biophys New York NY USA Columbia Univ Dept Biol Sci New York NY 10027 USA Univ Calif Riverside Dept Chem Riverside CA 92521 USA Baylor Coll Med Huffington Ctr Aging Houston TX 77030 USA Baylor Coll Med Dan L Duncan Comprehens Canc Ctr Houston TX 77030 USA

DNA methylation plays vital roles in both prokaryotes and eukaryotes. There are three forms of DNA methylation in prokaryotes: N-6-methyladenine (6mA), N-4-methylcytosine (4mC), and 5-methylcytosine (5mC). Although many sequencing methods have been developed to sequence specific types of methylation, few technologies can be used for efficiently mapping multiple types of methylation. Here, we present NT-seq for mapping all three types of methylation simultaneously. NT-seq reliably detects all known methylation motifs in two bacterial genomes and can be used for identifying de novo methylation motifs. NT-seq provides a simple and efficient solution for detecting multiple types of DNA methylation.

关键词： DNA methylation Next-generation sequencing Whole-genome epigenetic profiling

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Hierarchical flower-like manganese oxide/polystyrene with enhanced oxidase-mimicking performance for sensitive colorimetric detection of glutathione

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MICROCHIMICA ACTA 2022年第2期189卷 63-63页

作者： liu, Ying Yan, Jianghong Sun, Zhiheng Huang, Yu li, xuwen Jin, Yongri Jilin Univ Coll Chem 2699 Qianjin Rd Changchun 130012 Peoples R China Jilin Prov Acad Tradit Chinese Med Clin Hosp 1 Changchun 130021 Peoples R China

Glutathione (GSH) is an important antioxidant and free radical scavenger that converts harmful toxins into harmless substances and excretes them out of the body. In this paper, 3D hierarchical flower-like nanozyme named MnO2/PS (polystyrene) was successfully prepared by template method for the first time. After the systematical studies, MnO2/PS nanozyme was evaluated to possess favorable oxidase activity and direct 3,3',5,5'-tetramethylbenzidine (TMB) catalytic ability in the near-neutral environment at room temperature. With the addition of different concentrations of GSH, oxidized TMB can be reduced to TMB with the whole process from blue to nearly colorless be observed by naked eyes. In addition, there is a good linear relationship in the range 1-50 mu M and a detection limit of 0.08 mu M. The method proposed can be successfully applied to the detection of reduced GSH in tablets and injections with good selectivity and high sensitivity. The analysis results exhibited good consistency with the results obtained by HPLC.

关键词： Oxidase-like activity Manganese oxide Flower-like nanozyme GSH detection Colorimetric sensor

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Adaptive Temporal High-pass Infrared Non-uniformity Correction Algorithm Based on Guided Filter 2021

Adaptive Temporal High-pass Infrared Non-uniformity Correcti...

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7th International Conference on Computing and Artificial Intelligence, ICCAI 2021

作者： Zhang, Y.I. li, xuwen Zheng, Xin Wu, Qiang Beijing University of Technology Beijing China

ISBN: (纸本)9781450389501

In the scene-based infrared focal plane non-uniformity correction algorithm, the temporal high-pass filter non-uniformity correction algorithm is widely cited in various system platforms due to its low hardware platform requirements, but this algorithm is prone to "ghosting"problems and the corrected image still contains certain non-uniformity problems. To solve these problems, this paper proposes an adaptive temporal high-pass filter algorithm based on guided filter. The algorithm uses guided filter to accurately separate high-frequency components containing a large amount of fixed noise and image details, and performs image motion through adjacent frame residual images detection to determine whether to use high-frequency components for iterative operations in the temporal high-pass algorithm, and use the current image detail information to adaptively update the correction parameters of the temporal high-pass filter to avoid the accumulation of image detail information to complete the entire infrared non-uniformity correction . The algorithm in this paper uses actual infrared scene images to compare with the traditional temporal high-pass filter algorithm and bilateral filtering temporal high-pass algorithm. The results show that the algorithm proposed in this paper not only has a good correction effect, but also effectively suppresses the "ghosting"problem. © 2021 ACM.

关键词： High pass filters

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Regulation from gradient to near periodic sequence during anionic copolymerization of styrene and dimethyl-[4-(1-phenyl-vinyl)phenyl]silane (DPE-SiH)

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POLYMER 2022年 244卷 124663-124663页

作者： li, xuwen Han, li Zhang, Ruixue li, Chao Zhang, Songbo Bai, Hongyuan Wang, Xuefei Wang, Bo Ma, Hongwei Dalian Univ Technol Sch Chem Engn Dept Polymer Sci & Engn State Key Lab Fine Chem Dalian 116024 Peoples R China

A facile strategy for achieving sequence-controlled polymers with a sufficient molecular diversity is of great importance. The periodic and gradient copolymers of styrene (St) and dimethyl-[4-(1-phenylvinyl)phenyl]silane (DPE-SiH) obtained through the living anionic copolymerization (LACOP) under the variable feed ratios (St:DPE-SiH = 0.5-4) in a nonpolar solvent without additives were well studied in our prior work (Zhang et al., 2019) [34]. Herein, a further study on the critical feed ratio of St (S) and DPE-SiH (D) at 3:1 is performed during the LACOP, where a peculiar interest lies whether a novel sequence will emerge between the periodic and gradient sequence with the additives of t-BuOK and THF. As the whole chain propagation process is monitored using in situ H-1 NMR spectroscopy, the copolymerization kinetics, i.e., the reactivity ratio (r), apparent rate parameters (K-app.), and the copolymer microstructures are investigated. Both t-BuOK and THF favors the incorporation of St into LACOP, as the trends r(St) = 0.1 (gradient sequence without additives) < r(St) = 0.22-0.29 (near periodic sequence for t-BuOK) < r(St) = 0.7-0.76 (near periodic sequence for THF). The near periodic sequences exhibit different periodicities distributed as "DSSDSS " for the t-BuOK and "DSSSDSSS " for the THF in the major. The strategy achieves the sequence regulation from statistically gradient to near periodic copolymers. Furthermore, we observe that the near periodic copolymers exhibit the similar T-g values but over the gradient copolymers.

关键词： living anionic copolymerization Near periodic sequence Additives

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Chemical Constituents of the Marine Traditional Chinese Medicine of Pegasus laternarius Cuvier (Hai-E Yu)

Pharmaceutical Fronts

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Pharmaceutical Fronts 2024年第2期6卷 E149-E154页

作者： Sun, Shuqian Gao, Zhen Wang, Mengxue Chen, Sha Guo, Wenjuan li, xuwen School of Chemistry and Chemical Engineering University of Jinan Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug Discovery State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences

Pegasus laternarius Cuvier (Hai-E Yu) is a marine traditional Chinese medicine that has been used to treat cancers and reduce inflammation. Previous chemical investigations have only revealed the occurrence of high levels of protein, fatty acids, and a large number of steroids, thusmore active compounds in P. laternarius still need to be further discovered. The present study aims to search for new bioactive constituents of P. laternarius with cytotoxic effects and nitric oxide (NO) inhibitory activities. In this work, 16 pure compounds from the ethyl acetate fraction of Pegasus laternarius Cuvier were successively obtained by various chromatographic techniques, and the structure of the isolates was elucidated by spectroscopic analyses. The isolated and identified compounds included one 2H-1,2,3-triazole-4-carboxylate derivative (1), two oxadiazines (2, 3), two amino acids (4, 5), three nucleosides (6-8), three steroids (9-11), and five others (12-16). All the obtained compounds were evaluated for their antitumor activity on A549 and HCT-116 cell lines along with their inhibitory effects on lipopolysaccharide-induced NO production in RAW264.7 cells. The triazole compound 1 was found to exhibit moderate cytotoxicity against two human cell lines (A549 and HCT-116) with IC₅₀ values of 44.5 and 32.9 μmol/L, respectively. The steroid compound 10 inhibited NO production with IC₅₀ values lower than 50 μmol/L. Consequently, this study provides insight into the cytotoxic and NO inhibitory activities of the marine traditional Chinese medicines in Hai-E-Yu. © 2024. The Author(s).

关键词： anti-inflammatory cytotoxicity marine traditional Chinese medicine Pegasus laternarius Cuvier

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Assessing the prognostic impact of prostatic urethra involvement and developing a nomogram for T1 stage bladder cancer

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BMC UROLOGY 2023年第1期23卷 182-182页

作者： Wan, Hao Zhan, Xiangpeng li, xuwen Chen, Tao Deng, Xinxi liu, Yang Deng, Jun Fu, Bin li, Yu Nanchang Univ Dept Urol Affiliated Hosp 1 Nanchang Jiangxi Peoples R China Nanchang Univ Dept Cardiol Affiliated Hosp 2 Nanchang Jiangxi Peoples R China Jiu Jiang NO1 Peoples Hosp Dept Urol Jiujiang Jiangxi Peoples R China

Purpose To investigate prognostic values of prostatic urethra involvement (PUI) and construct a prognostic model that estimates the probability of cancer-specific survival for T1 bladder cancer patients. Method and materials We investigated the national Surveillance, Epidemiology, and End Results (SEER) database (2004-2015) to get patients diagnosed with T1 bladder cancer. An external validation cohort was obtained from the First Affiliated Hospital of Nanchang University. The Kaplan-Meier method with the log-rank test was applied to assess cancer-specific survival (CSS) and overall survival (OS). Moreover, the propensity score matching (PSM) and multivariable Cox proportional hazard model were performed. All patients were randomly divided into the development cohort and validation group at the ratio of 7:3. The performance of the model was internally validated by calibration curves and the concordance index (C-index). Results The PUI group had a lower survival rate of both CSS and overall survival OS before and after PSM when compared to non-involved patients (All P < 0.05). Multivariate analysis revealed a poor prognosis in the PUI group for cancer-specific mortality (CSM) and all-cause mortality (ACM) analyses before and after PSM (All P < 0.05). Seven variables, including age, surgery, radiotherapy, tumour size, PUI, and marital status, were incorporated in the final nomogram. The C-index in the development cohort was 0.715 (0.711-0.719), while it was 0.672 (0.667-0.677) in the validation group. Calibration plots for 3- and 5-year cancer-specific survival showed good concordance in the development and validation cohorts. Conclusions PUI was an independent risk factor of ACM and CSM in T1 bladder cancer patients. In addition, a highly discriminative and precise nomogram that predicted the individualized probability of cancer-specific survival for patients with T1 bladder cancer was constructed.

关键词： Prognosis T1 bladder cancer Prostatic urethra involved SEER Nomogram

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染料防尘剂的制备与研究

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印染助剂 2024年第10期41卷 28-31页

作者：李旭文王金剑传化智联股份有限公司浙江杭州311215

提升防尘剂中高沸点、高黏度组分含量,引入合适HLB值生物基乳化剂和双子型表面活性剂,可以降低防尘剂在高温下的挥发度,提高其防尘性能、耐电解质性能、储存稳定性和乳化性能,且对染料色光没有影响,具有良好的应用推广前景和市场竞争力。

关键词：防尘剂生物基乳化剂双子型表面活性剂乳化

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体育器材专用尼龙6复合材料的3D打印丝材制备与表征

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塑料助剂 2023年第1期 24-26,60页

作者：张琳李绪稳咸阳师范学院咸阳712000 西藏民族大学咸阳712082

为增强尼龙6在体育器材器件3D打印应用中的适用性,本次研究采用滑石粉对尼龙6实施改性处理,制备出可直接用于3D打印的滑石粉改性PA6复合丝材。通过微观形貌观测的方式来确定滑石粉的合理添加量,并对滑石粉合理添加量下改性PA6复合丝材... 详细信息

为增强尼龙6在体育器材器件3D打印应用中的适用性,本次研究采用滑石粉对尼龙6实施改性处理,制备出可直接用于3D打印的滑石粉改性PA6复合丝材。通过微观形貌观测的方式来确定滑石粉的合理添加量,并对滑石粉合理添加量下改性PA6复合丝材的力学性能进行测试。经实验研究发现,本次研究所制备的尼龙6复合3D打印丝材相比于单纯尼龙6和市售e-PA6丝材来说,整体力学性能得到显著提升,在体育器材设备轻质化方面具有一定的应用价值。

关键词：尼龙6 微观形貌观测 3D打印丝材力学性能

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Transcytosis Mediated Deep Tumor Penetration for Enhanced Chemotherapy and Immune Activation of Pancreatic Cancer

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ADVANCED FUNCTIONAL MATERIALS 2023年第28期33卷

作者： Chen, Hongyi Song, Haolin Luo, Yifan li, Chufeng Wang, Yu liu, Jie Luo, Feifei Fan, Hongrui li, xuwen Sun, Tao Jiang, Chen Fudan Univ Sch Pharm Dept Pharmaceut Key Lab Smart Drug DeliveryState Key Lab Med Neur Shanghai 201203 Peoples R China Fudan Univ MOE Frontiers Ctr Brain Sci Shanghai 201203 Peoples R China Fudan Univ Huashan Hosp Dept Digest Dis Shanghai 200040 Peoples R China

The hyperproliferative tumor stroma of pancreatic ductal adenocarcinoma (PDAC) severely limits drug permeation and constructs an immunosuppressive microenvironment, causing resistance to chemotherapy and immunotherapy. Traditional nanomedicine mainly focuses on manipulating nanoparticles' particle size or electrical characteristics to penetrate deep PDAC through the paracellular pathway, but the transcellular pathway is often ignored. Therefore, a versatile drug-polymer conjugate PODEA-Gem-HMI is prepared and assembled into nanoparticles for the codelivery of chemotherapy drug gemcitabine and focal adhesion kinase (FAK) inhibitor defactinib. While sensing the mild acidity in the tumor microenvironment, the nanoparticle will disintegrate and release defactinib to modulate the tumor stroma. The PODEA block of the conjugate can bind with cell membranes reversibly and trigger adsorption-mediated transcytosis (AMT) for promoted tumor penetration and cellular uptake. The internalized conjugates will release gemcitabine responding to the overexpressed glutathione (GSH) for enhanced chemotherapy, and PHMI can condensate the STING monomers for prolonged spontaneous immune stimulation.

关键词： sting activation stroma modulation transcytosis tumor penetration

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Recent advances in the synthesis of biodegradable polyesters by sustainable polymerization: lipase-catalyzed polymerization

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RSC ADVANCES 2020年第59期10卷 36230-36240页

作者： liu, Ying Song, lijie Feng, Na Jiang, Wei Jin, Yongri li, xuwen Jilin Univ Coll Chem 2699 Qianjin Rd Changchun 130012 Jilin Peoples R China Jilin Prov Acad Tradit Chinese Med Clin Hosp 1 Changchun 130021 Peoples R China Zhengzhou Univ Acad Med Sci Dept Mol Pathol Applicat Ctr Precis MedAffiliated Hosp 2 Zhengzhou 450052 Henan Peoples R China

Over the past three decades, enzymatic polymerization has dramatically developed and gradually broadened as a creative methodology in the construction of polymeric materials with tailor-made structures and properties. Compared with transition metal catalyst polymerizations, enzymatic polymerization is more attractive in the biomedicine field due to the metal-free residue, good biocompatibility, and few by-products. Meanwhile, enzymatic polymerization has far more activity towards macrolides. In this review, the synthesis of lipase-catalyzed polymer materials is systematically summarized, focusing on the synthesis of the complex and well-defined polymers. The enzymatic polyester synthesis was then discussed concerning the different reaction types, including ring-opening polymerization, polycondensation, a combination of ring-opening polymerization with polycondensation, and chemoenzymatic polymerization. Besides, exploration of novel biocatalysts and reaction media was also described, with particular emphasis on the enzymes obtainedviaimmobilization or protein engineering strategies, green solvents, and reactors. Finally, recent developments in catalytic kinetics and mechanistic studies through the use of spectroscopy, mathematics, and computer techniques have been introduced. Besides, we addressed the remaining central issues in enzymatic polymerization and discussed current studies aimed at providing answers.

关键词： Ring opening polymerization

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