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A highly sensitive one-step nanobody-based immunoassay to specifically detect antibodies against fowl adenovirus serotype 4

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POULTRY SCIENCE 2025年第4期104卷 104970页

作者： Ji, Pinpin zhang, Hao Xiri, Yangzong Li, Ziling Liu, Zhixiang Dan, Miao Li, Xiaoxuan Sun, xuwen Zhao, Qin Sun, Yani Northwest A&F Univ Coll Vet Med Dept Prevent Vet Med Yangling 712100 Shaanxi Peoples R China Minist Agr Sci Observing & Expt Stn Vet Pharmacol & Diagnost Yangling 712100 Shaanxi Peoples R China

Hepatitis-hydropericardium syndrome, caused by fowl adenovirus serotype 4 (FAdV-4), has resulted in significant economic damage to the poultry industry. To monitor viral exposure and vaccine efficacy, some traditional antibody-based immunoassays have been developed for detecting anti-FAdV-4 antibodies. However, these assays have some drawbacks including multi-step operations and higher production cost. Recently, nanobodies are regarded as a promising tool for developing immunoassays. In the study, 23 nanobodies against FAdV-4 were screened and expressed with horseradish peroxidase (HRP) in the HEK293T cells. Then, the FAdV-4-Nb28-HRP fusion protein was selected for developing competitive enzyme-linked immunoassays (cELISA) to detect antiFAdV-4 antibodies in the chicken sera. The optimal concentrations and dilutions for the coating antigen, fusion protein and testing sera were determined to be 400 ng/well, 1:80 and 1:20, respectively. After the coated plates were vacuumized and stored, the operation of cELISA to detect clinical chicken sera was only one-step and the full time was 75 min. The cELISA also exhibited high sensitivity, specificity, reproducibility and good agreement with the commercial ELISA kit. When the sequential sera from the challenged chickens were tested, the cELISA showed superior sensitivity compared with the commercial ELISA kit. Moreover, epitope mapping revealed that the nanobody specifically recognized the sites GLN235 ASN236 SER238 of the fiber-1 protein, highly conserved among different FAdV-4 isolates and different from the FAdV-1 and-8. The results indicated that cELISA can specifically detect anti-FAdV-4 antibodies. Collectively, the developed one-step nanobody-based cELISA is an ideal method for epidemiological investigation and vaccine immune evaluation of FAdV-4.

关键词： Nanobody One-step competitive ELISA FAdV-4 antibodies Fiber-1 protein

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Study on Flow Behavior and Adsorption Characteristics of Encapsulated Polymer Particles

Study on Flow Behavior and Adsorption Characteristics of Enc...

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2025 International Petroleum Technology Conference, IPTC 2025

作者： Liu, Yongsheng Wei, Bei Hou, Jian Zheng, Ningyu Qin, xuwen zhang, Yang Guo, Zhixin zhang, Dequan National Key Laboratory of Deep Oil and Gas China University of Petroleum East China Shandong Qingdao China School of Petroleum Engineering China University of Petroleum East China Shandong Qingdao China

ISBN: (纸本)9781959025436

Encapsulated polymer refers to Micro/Nano-scale particles with polymer as the core, which can release the polymer to achieve thickening. Encapsulated polymer flooding is a new type of deep profile control technology suitable for further improving the oil recovery of conventional reservoirs. However, the adsorption and temporary blockage behaviors of encapsulated polymer particles in porous media significantly affect the accurate description of their targeted viscosity enhancement. On this account, we investigated how encapsulated polymer particles adsorb and temporarily block. Using PDMS material microfluidic chips, we investigated the flow behavior of encapsulated polymer in seven different pore throat structures. High-precision pressure sensors and microscopes were employed to real-time capture microscopic flow images of microcapsule particles inside single micro-channels and observe pressure fluctuation characteristics. Based on particle image velocimetry (PIV) systems, we studied the diverting fluid behavior in parallel micro-channels with different width ratios. Building on this, we conducted dynamic adsorption experiments to quantitatively characterize the adsorption and retention behavior of encapsulated polymer particles in porous media. In this study, we observed four phenomena of free flow, surface adsorption, blockage, and multi-particle aggregation slug flow during the flow of encapsulated polymer particles in a single microchannel. In parallel microchannels, the capsule particles exhibited selectivity towards flow paths, primarily entering wider throats. With the increase of the width ratio, the encapsulated polymer particles in the narrow throat are more confined and are more likely to accumulate in the throat and cause blockage. Experiments in porous media show that the adsorption and retention of capsule particles are inversely proportional to the permeability. The particles are more inclined to flow along the high permeability dominant channel. The

关键词： Microfluidics

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油煤浆黏温特性研究进展

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化工进展 2019年第9期38卷 4003-4011页

作者：张彦军舒歌平章序文高山松王洪学中国神华煤制油化工有限公司上海研究院煤炭直接液化国家工程实验室

煤直接液化技术是缓解石油供需矛盾、实现煤炭清洁高效利用的重要技术途径之一,油煤浆的黏温特性对其配制、输送、加压、预热及反应性能具有重要影响。本文从油煤浆黏温特性的变化规律、研究方法、变化机理、影响因素等方面的研究进展... 详细信息

煤直接液化技术是缓解石油供需矛盾、实现煤炭清洁高效利用的重要技术途径之一,油煤浆的黏温特性对其配制、输送、加压、预热及反应性能具有重要影响。本文从油煤浆黏温特性的变化规律、研究方法、变化机理、影响因素等方面的研究进展进行了综述。在升温过程中,油煤浆流变特性表现为非牛顿流体,具有剪切稀化特性;高温高压下黏度测定方法主要有4种;分析黏温特性变化机理:煤浆制备和升温阶段黏度变化主要由溶胀作用导致,高温阶段主要由煤裂解产生沥青质(尤其是前沥青烯)导致;从溶剂、煤的性质(煤阶、显微组分及粒径分布)、剪切速率、油煤浆浓度、催化剂和氢分压等方面分析了油煤浆黏温特性的影响因素。最后指出了未来油煤浆黏温特性的研究方向,为我国煤直接液化技术的进一步发展提供参考。

关键词：煤直接液化油煤浆黏温特性变化机理影响因素

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我国保障性住房“有效供给”的悖论与破解

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新疆社会科学 2020年第6期 127-133,145页

作者：李永安张旭文宜春学院经济管理学院江西宜春336000 江西财经大学经济学院江西南昌330013

我国保障性住房制度对彰显社会主义公平价值、弥补房改后的房价非理性上涨之弊意义重大。但是,我国保障性住房供给存在着有效性不足问题,至少在保障性住房制度实施过程中陷入四重悖论。一是"可负担性"成本控制与保障房制度可... 详细信息

我国保障性住房制度对彰显社会主义公平价值、弥补房改后的房价非理性上涨之弊意义重大。但是,我国保障性住房供给存在着有效性不足问题,至少在保障性住房制度实施过程中陷入四重悖论。一是"可负担性"成本控制与保障房制度可持续要求的悖论;二是保障房产权"完整性"流转要求与产权"约束性"工具的悖论;三是保障户身份的信用不足与保障户较高住房金融需求的悖论;四是保障户获得住房保障资格与导致更高生活工作成本的悖论。为此,要通过机制创新破解四重悖论,增强我国保障性住房供给的有效性。

关键词：保障性住房有效供给成本控制产权约束悖论

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适应与重构:校企创新主体资源融合路径研究

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中国科技论坛 2022年第9期 63-72页

作者：迟景明何声升张旭雯大连理工大学高等教育研究院辽宁大连116024

校企协同创新主体适应性行为是实现资源融合的重要途径,资源融合重构和深化了校企创新主体之间的协同关系。基于适应性行为视角分析校企创新主体资源融合的内在逻辑,即资源融合的过程就是适应性行为的作用过程。而适应性行为建构创新主... 详细信息

校企协同创新主体适应性行为是实现资源融合的重要途径,资源融合重构和深化了校企创新主体之间的协同关系。基于适应性行为视角分析校企创新主体资源融合的内在逻辑,即资源融合的过程就是适应性行为的作用过程。而适应性行为建构创新主体资源融合的规则体系,同时适应性行为要嵌入到资源融合的规则体系中,并受规则体系所制约。然后基于扎根理论的探索性案例分析在适应性行为的作用下,创新资源需经历纳新、适应与再造3种状态,校企创新主体资源融合的过程就是在吸收、重构与再造的作用下螺旋式前进的过程。

关键词：适应性行为资源融合资源吸收资源重构资源再造

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Acute Effects of Different Electroacupuncture Point Combinations to Modulate the Gut-Brain Axis in the Minipig Model

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EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2022年第1期2022卷 4384693页

作者： zhang, xuwen Guerin, Sylvie Launay, Youenn Serrand, Yann Coquery, Nicolas Val-Laillet, David Guangzhou Univ Chinese Med Guangzhou Peoples R China Guangzhou Rui Xingtang Chinese Med Clin Guangzhou Peoples R China Univ Rennes INRAE INSERM Nutr Metabolisms & CancNuMeCan Rennes France

This study aimed to compare the gut-brain axis responses to acute electroacupuncture (EA) at different acupoint combinations in the minipig model. Four adult Yucatan minipigs were subjected twice to four acute EA treatments (25-minute acute sessions) including sham (false acupoints) and control (no EA), during anesthesia and according to a Latin-square design paradigm. Acupoint combinations (4 loci each) are head-abdomen (#70 Dafengmen, #35 Sanwan), back (bilateral #27 Pishu, #28 Weishu), leg (bilateral #79 Hangou, #63 Housanli), and sham (2 bilateral points that are not acupoints). Electrocardiograms were performed to explore heart rate variability (HRV). Infrared thermography was used to measure skin temperature at the stimulation points. Saliva (cortisol) and blood samples (leptin, total/active ghrelin, insulin, and glucose) were collected for further analyses before and after acute EA. All animals were also subjected to BOLD fMRI to investigate the brain responses to EA. Acute EA significantly modulated several physiological and metabolic parameters compared to basal, sham, and/or control conditions, with contrasting effects in terms of BOLD responses in brain regions involved in the hedonic and cognitive control of food intake. The head-abdomen combination appeared to be the most promising combination in terms of brain modulation of the corticostriatal circuit, with upregulation of the dorsolateral prefrontal cortex, dorsal striatum, and anterior cingulate cortex. It also induced significantly lower plasma ghrelin levels compared to sham, suggesting anorectic effects, as well as no temperature drop at the stimulation site. This study opens the way to a further preclinical trial aimed at investigating chronic EA in obese minipigs.

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Direct Arylation Polycondensation-Derived Polythiophene Achieves Over 16% Efficiency in Binary Organic Solar Cells via Tuning Aggregation and Miscibility

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ADVANCED ENERGY MATERIALS 2024年第47期14卷

作者： zhang, xuwen zhang, Tao Liang, Ziqi Shi, Yibo Li, Saimeng Xu, Chenhui Li, Miaomiao Ye, Long Hou, Jianhui Geng, Yanhou Tianjin Univ Sch Mat Sci & Engn Tianjin Key Lab Mol Optoelect SciKey Lab Organ In Minist EducCollaborat Innovat Ctr Chem Sci & Engn Tianjin 300350 Peoples R China Chinese Acad Sci Inst Chem CAS Res Educ Ctr Excellence Mol Sci State Key Lab Polymer Phys & ChemBeijing Natl Lab Beijing 100190 Peoples R China

Polythiophenes are the most appealing donor materials in organic solar cells (OSCs) due to their simple chemical structures. However, the top-performance polythiophenes are typically synthesized via Stille polycondensation, which is problematic due to significant toxicity and poor atom economy. By contrast, direct arylation polycondensation (DArP) is an eco-friendly, and atom-efficient alternative for synthesizing conjugated polymers, while the best efficiency for DArP-derived polythiophenes is below 12%. This study reports a series of polythiophene-based donors synthesized via DArP. Among these, PT4F-Th reaches a power conversion efficiency (PCE) of 16.4%, which not only matches the current record for polythiophene-based donor materials, but also marks the highest PCE achieved by DArP-derived donors to date. The superior performance of PT4F-Th is largely attributed to its optimal temperature-dependent aggregation behavior and moderate miscibility with acceptors, along with the highest crystallinity among the candidates, resulting in the most favorable blend film morphology. This study underscores the significant potential of DArP-derived polythiophenes in developing high-performance and eco-friendly OSCs. A series of polythiophene-based donors are synthesized using direct arylation polycondensation (DArP), highlighting PT4F-Th, which achieves an outstanding power conversion efficiency (PCE) of 16.4% in binary organic solar cells (OSCs). This efficiency sets a new record for DArP-derived donors. Blend film morphology is characterized by X-ray scattering and microscopy, emphasizing the synergistic effects of temperature-dependent aggregation behavior and miscibility. image

关键词： direct arylation polycondensation miscibility organic solar cells polythiophene power conversion efficiency

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吡啶抽提对不同显微组分煤结构特征的影响

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煤炭转化 2014年第2期37卷 1-5页

作者：蔺华林李克健章序文中国神华煤制油化工有限公司上海研究院上海博士高级工程师201108

对神东上湾煤(SDR)及由其岩相分离得到的镜质组富集物(SDV)和惰质组富集物(SDI)进行了吡啶抽提,得到不同煤样的抽提率,并对抽提前后的煤样进行了13C-NMR,FTIR和XRD分析表征.结果表明,三种煤样的抽提率(质量分数)顺序为:镜质组富集物(15.... 详细信息

对神东上湾煤(SDR)及由其岩相分离得到的镜质组富集物(SDV)和惰质组富集物(SDI)进行了吡啶抽提,得到不同煤样的抽提率,并对抽提前后的煤样进行了13C-NMR,FTIR和XRD分析表征.结果表明,三种煤样的抽提率(质量分数)顺序为:镜质组富集物(15.78%)>上湾煤(13.89%)>惰质组富集物(9.45%);通过对吡啶抽提前后煤样的13C-NMR,FTIR和XRD的分析表明,吡啶抽提后三种煤样的芳香度均有所增加,抽提前后煤样的骨架结构没有发生大的变化.

关键词：煤结构吡啶抽提镜质组富集物惰质组富集物 13C-NMR

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Nomogram to screen older adult patients attending the radiology department for sarcopenia

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BMC GERIATRICS 2025年第1期25卷 1-9页

作者： Yuan, Guiying Ye, Guoxi Hu, Jianguang Hu, Huimin Shi, Chanmei zhang, Ye Huang, Junbing Li, Zhiqiong Zeng, xuwen Tan, Rongshao Xiong, Yuchao Jinan Univ Guangzhou Red Cross Hosp Dept Resp Guangzhou Peoples R China Jinan Univ Guangzhou Red Cross Hosp Dept Radiol 396 Tongfu Rd Guangzhou Guangzhou 510220 Guangdong Provi Peoples R China Jinan Univ Guangzhou Red Cross Hosp Dept Nephrol Guangzhou Peoples R China Jinan Univ Guangzhou Red Cross Hosp Inst Dis Oriented Nutr Res Dept Clin Nutr Guangzhou Peoples R China

BackgroundSarcopenia is an age-related syndrome that can impact the physical and mental health of older adults. However, it is often overlooked in clinical practice. Therefore, we aim to construct a nomogram based on simplified discriminant parameters for screening older adult patients for sarcopenia *** cross-sectional study included 654 patients aged >= 60 years who underwent an examination in the radiology department between October 2023 and June 2024. Patients were diagnosed with sarcopenia according to the method and cutoff value criteria proposed the Asian Working Group on Sarcopenia (AWGS) 2019 criteria. Calf circumference (CC), SARC-F score, mid-upper arm circumference (MUAC), and SARC-CalF score were used as simplified discriminant parameters for sarcopenia. The discriminative ability of these parameters for sarcopenia was assessed using receiver operating characteristic analysis. Additionally, we included each screening parameter and evaluated it's important for screening for the presence of sarcopenia via univariate and multivariate logistic regression analysis to develop a new screening nomogram model. The performance of the nomogram was evaluated using receiver operating characteristic curves, and the performance of the nomogram model was compared to that of CC, SARC-F, MUAC, and the SARC-CalF using the Delong *** the 654 subjects, 120 (18.3%) were diagnosed with sarcopenia, and the areas under the curve (AUCs) of the CC, SARC-F, MUAC, and SARC-CalF were 0.73, 0.61, 0.66, and 0.70, respectively. The multivariate analysis results revealed that older age, male sex, low CC, low MUAC, and low strength were related to sarcopenia. A nomogram model constructed with these five variables had an AUC of 0.84. The DeLong test showed that the diagnostic efficacy of the joint model was significantly higher than that of CC, SARC-F, MUAC, and *** simple nomogram based on simplified discriminant parameters offers personalized

关键词： Sarcopenia Nomogram Calf circumference SARC-F SARC-CalF

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A synergistic water-saving strategy combining stage-specific irrigation and application of exogenous thiamine enhances drought resistance of Xinjiang cotton

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PLANT AND SOIL 2025年 1-14页

作者： Zhao, Fuxiang Xia, Linjie Sun, Simin Li, Hang Rong, Yuxuan Wang, Yuxin Wang, xuwen Kong, Xianhui Zhu, Longfu zhang, Xianlong Yu, Yu Yang, Xiyan Xinjiang Acad Agr & Reclamat Sci Cotton Inst Shihezi 832000 Xinjiang Peoples R China Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan 430070 Hubei Peoples R China Hubei Hongshan Lab Wuhan Peoples R China

Background and aimsXinjiang is the largest cotton planting area in China. However, the perennial water shortage in Xinjiang restricts the development of the cotton industry, making drought one of the important limiting factors. The aim of this study was to evaluate the effectiveness of water-saving scheme and thiamine (Thi) application on alleviating the adverse effects of drought stress on the growth and development of *** this study, five Xinjiang-released cotton varieties (Jinken1775, Jinken1565, Huamian702, Zhongmian979, and Jinfeng2) were selected as the research object, and Xinluzao8 served as the drought-sensitive control. Comprehensive drought resistance index (D) of the drought resistance coefficient (DRC) of four yield and four quality traits were used to evaluate the drought resistant ability of the six cotton varieties under different water conditions and Thi spraying *** stage-specific irrigation strategy (60% during vegetative vs. 90% during the flowering and boll stage) concurrently maintained higher drought resistance across cultivars, with Jinken1565 exhibited the highest drought resistance. This scheme reduced irrigation by 26% while sustaining fiber quality. Exogenous 50 mM Thi further enhanced drought tolerance, reducing transpiration rate by 33% and blade damage by 55%. These results demonstrate that stage-specific water management optimizes drought adaptation in cotton cultivation under drought *** study suggests that a 26% water-saving scheme was proposed for most drought-resistant varieties planted in Xinjiang, and an appropriate scheme to achieve stable yield by Thi application during the early drought condition was proposed for varieties with low drought resistant ability.

关键词： Cotton Drought resistance Comprehensive drought resistance index Thiamine Water saving scheme

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