Disclosed is a method for purifying and concentrating a foot-and-mouth disease virus inactivated antigen, comprising the steps of: (1) adding an adaptor protein with the amino acid sequence as shown in SEQ ID NO: 2 in...
标准号:
WO2018137323(A1)
Disclosed is a method for purifying and concentrating a foot-and-mouth disease virus inactivated antigen, comprising the steps of: (1) adding an adaptor protein with the amino acid sequence as shown in SEQ ID NO: 2 into the foot-and-mouth disease virus inactivated antigen, mixing homogeneously, and incubating;(2) adding a purified carrier, mixing homogeneously, and incubating the mixture, wherein the purified carrier is the skeleton of Lactococcus lactis;and (3) centrifuging and obtaining a precipitate. The purifying and concentrating method can efficiently purify the foot-and-mouth disease virus inactivated antigen and efficiently remove impurities, and has a high antigen recovery efficiency, large concentration multiple, low equipment requirement, simple operation, and low cost.
The present invention provides a compound immunopotentiator, containing 5-520 μg/mL of monophosphoryl lipid, 10-520 μg/mL of muramyl dipeptide, 1-520 μg/mL of beta-glucan, and 0.05-5.2 mg/mL of Astragalus polysacch...
标准号:
WO2018227728(A1)
The present invention provides a compound immunopotentiator, containing 5-520 μg/mL of monophosphoryl lipid, 10-520 μg/mL of muramyl dipeptide, 1-520 μg/mL of beta-glucan, and 0.05-5.2 mg/mL of Astragalus polysaccharide. The present invention also provides a foot-and-mouth disease inactivated vaccine containing said compound immunopotentiator, and a method for preparing the compound immunopotentiator and the foot-and-mouth disease inactivated vaccine.
BACKGROUND: Postpartum hemorrhage (PPH) is the most common cause of parturient death worldwide [1]. However, most cases of PPH can be avoided. OBJECTIVE: This paper employs statistical methods to screen risk factors o...
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BACKGROUND: Postpartum hemorrhage (PPH) is the most common cause of parturient death worldwide [1]. However, most cases of PPH can be avoided. OBJECTIVE: This paper employs statistical methods to screen risk factors of PPH and calculate relevant parameters. METHODS: Multivariable logistic regression analysis was applied to obtain the regression equation and odds ratio (OR) value. The determined risk factors were assigned comprehensive and reasonable scores according to scientific relevance and reasoning according to the OR value. P-PPH values were calculated in order to assess the morbidity of PPH. RESULTS: The scores of pregnant women could be intuitively used to show the risk of getting PPH. CONCLUSIONS: Through the above methods, a comprehensive risk evaluation method of detecting PPH was developed.
G protein-coupled receptor, family C, group 5, member A (GPRC5A) had received attentions for its role in carcinogenesis and prognostic values in several types of cancer. However, the functional roles of GPRC5A in gast...
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G protein-coupled receptor, family C, group 5, member A (GPRC5A) had received attentions for its role in carcinogenesis and prognostic values in several types of cancer. However, the functional roles of GPRC5A in gastric cancer (GC) had never been elucidated. The expression levels of GPRC5A were detected by real-time quantitative reverse transcription PCR and Western blot in GC tissues and adjacent non-tumor tissues. GPRC5A expression in tissue sections of 106 GC samples was evaluated using immunohistochemistry. The staining results were compared with clinicopathological factors and to the prognosis of GC patients. The mRNA and protein expression levels of GPRC5A in gastric cancer tissues were higher than those in adjacent non-tumor tissues. Positive GPRC5A expression was significantly correlated with larger size of primary tumor, diffuse type (Lauren's classification), deeper serosal invasion, and more lymph node metastasis. In addition, Kaplan-Meier curve analysis demonstrated that GC patients with positive GPRC5A expression had poor prognosis than those with negative GPRC5A expression. GPRC5A expression was identified as an independent factor of the overall survival in GC patients by multivariate Cox analysis. Further, the overall survival difference existed between patients with GPRC5A positive and negative groups in GC patients with lymph node metastasis. Our results suggested that elevated levels of GPRC5A played significant roles in GC progression. GPRC5A could serve as a prognostic biomarker of GC.
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