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A Micro-Environment Regulator for Filling the Clinical Treatment Gap after a Glioblastoma Operation

ADVANCED HEALTHCARE MATERIALS 2022年第3期11卷 2101578-2101578页

作者： Zhang, Yiwen You, Haoyu Wang, Yaoben Chen, Qinjun Guo, Qin Chu, Yongchao Li, Chao Zhou, Wenxi Chen, Hongyi Liu, Peixin Wang, Yu Zhao, Zhenhao Zhou, zheng Luo, Yifan Li, xuwen Zhang, Tongyu Song, Haolin Li, Chufeng Su, Boyu Sun, Tao Bi, Yunke Yu, Lin Jiang, Chen Fudan Univ Sch Pharm Dept PharmaceutState Key Lab Med Neurobiol & MOE Inst Brain SciMinist EducKey Lab Smart Drug Del Shanghai 201203 Peoples R China Fudan Univ Dept Macromol Sci State Key Lab Mol Engn Polymers Shanghai 200438 Peoples R China Shanghai Jiao Tong Univ Sch Med Shanghai Gen Hosp Dept Neurosurg Shanghai 201203 Peoples R China

The rapid postoperative recurrence and short survival time of glioblastoma (GBM) patients necessitate immediate and effective postoperative treatment. Herein, an immediate and mild postoperative local treatment strategy is developed that regulates the postoperative microenvironment and delays GBM recurrence. Briefly, an injectable hydrogel system (imGEL) loaded with Zn(II)(2)-AMD3100 (AMD-Zn) and CpG oligonucleotide nanoparticles (CpG NPs) is injected into the operation cavity, with long-term function to block the recruitment of microglia/ macrophages and activate cytotoxic T cells. The finding indicated that the imGEL can regulate the immune microenvironment, inhibit GBM recurrence, and gain valuable time for subsequent adjuvant clinical chemotherapy.

关键词： drug delivery glioblastoma injectable hydrogels microenvironments post-operative therapy

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Surface-displayed porcine reproductive and respiratory syndrome virus from cell culture onto gram-positive enhancer matrix particles

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JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2018年第10期45卷 889-898页

作者： Li, Lan Qiao, xuwen Chen, Jin Zhang, Yuanpeng zheng, Qisheng Hou, Jibo Jiangsu Acad Agr Sci Ctr Engn & Technol Vet Biol Natl Res Nanjing 210014 Jiangsu Peoples R China Jiangsu Coinnovat Ctr Prevent & Control Important Yangzhou 225009 Jiangsu Peoples R China

Vaccine immunization is now one of the most effective ways to control porcine reproductive and respiratory syndrome virus (PRRSV) infection. Impurity is one of the main factors affecting vaccine safety and efficacy. Here we present a novel innovative PRRSV purification approach based on surface display technology. First, a bifunctional protein PA-GRFT (protein anchor-griffithsin), the crucial factor in the purification process, was successfully produced in Escherichia coli yielding 80mg/L of broth culture. Then PRRSV purification was performed by incubation of PA-GRFT with PRRSV and gram-positive enhancer matrix (GEM) particles, followed by centrifugation to collect virions loaded onto GEM particles. Our results showed that most of the bulk impurities had been removed, and PA-GRFT could capture PRRSV onto GEM particles. Our lactic acid bacteria-based purification method, which is promising as ease of operation, low cost and easy to scale-up, may represent a candidate method for the large-scale purification of this virus for vaccine production.

关键词： Lactic acid bacteria GEM particles Purification PRRSV Griffithsin

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Biomimetic Dendrimer-Peptide Conjugates for Early Multi-Target Therapy of Alzheimer's Disease by Inflammatory Microenvironment Modulation

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ADVANCED MATERIALS 2021年第26期33卷 2100746-2100746页

作者： Liu, Peixin Zhang, Tongyu Chen, Qinjun Li, Chao Chu, Yongchao Guo, Qin Zhang, Yiwen Zhou, Wenxi Chen, Hongyi Zhou, zheng Wang, Yu Zhao, Zhenhao Luo, Yifan Li, xuwen Song, Haolin Su, Boyu Li, Chufeng Sun, Tao Jiang, Chen Fudan Univ Key Lab Smart Drug Delivery Minist Educ State Key Lab Med Neurobiol Shanghai 201203 Peoples R China Fudan Univ MOE Frontiers Ctr Brain Sci Dept Pharmaceut Sch Pharm Shanghai 201203 Peoples R China

Current therapeutic strategies for Alzheimer's disease (AD) treatments mainly focus on beta-amyloid (A beta) targeting. However, such therapeutic strategies have limited clinical outcomes due to the chronic and irreversible impairment of the nervous system in the late stage of AD. Recently, inflammatory responses, manifested in oxidative stress and glial cell activation, have been reported as hallmarks in the early stages of AD. Based on the crosstalk between inflammatory response and brain cells, a reactive oxygen species (ROS)-responsive dendrimer-peptide conjugate (APBP) is devised to target the AD microenvironment and inhibit inflammatory responses at an early stage. With the modification of the targeting peptide, this nanoconjugate can efficiently deliver peptides to the infected regions and restore the antioxidant ability of neurons by activating the nuclear factor (erythroid-derived 2)-like 2 signaling pathway. Moreover, this multi-target strategy exhibits a synergistic function of ROS scavenging, promoting A beta phagocytosis, and normalizing the glial cell phenotype. As a result, the nanoconjugate can reduce ROS level, decrease A beta burden, alleviate glial cell activation, and eventually enhance cognitive functions in APPswe/PSEN1dE9 model mice. These results indicate that APBP can be a promising candidate for the multi-target treatment of AD.

关键词： Alzheimer&apos s disease antioxidant ability glial cell activation inflammatory responses multi‐ target treatments synergistic function

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High Q and high gradient performance of the first medium-temperature baking 1.3 GHz cryomodule

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Physical Review Accelerators and Beams 2024年第9期27卷 092003页

作者： Weimin Pan Jiyuan Zhai Feisi He Rui Ge zhenghui Mi Peng Sha Song Jin Ruixiong Han Qunyao Wang Haiying Lin Guangwei Wang xuwen Dai Zhanjun Zhang Mei Li Minjing Sang Liangrui Sun Rui Ye Tongxian Zhao Shaopeng Li Keyu Zhu Baiqi Liu Xiaolong Wang Xiangchen Yang Xiaojuan Bian Xiangzhen Zhang Huizhou Ma Jianbing Zhao Liang Zhang Hui Zhao Runbing Guo Zhihui Mu Conglai Yang Xiaobing zheng Chao Dong Hongjuan zheng zhengze Chang Xiaochen Yang Tongming Huang Qiang Ma Zihan Wang Ming Liu Wenzhong Zhou Senyu Chen Chinese Academy of Sciences Beijing 100049 China Beijing 100049 China

The world’s first 1.3 GHz cryomodule containing eight 9-cell superconducting radio-frequency (rf) cavities treated by medium-temperature furnace baking (mid-T bake) was developed at the Institute of High Energy Physics, Chinese Academy of Sciences. The 9-cell cavities in the cryomodule achieved an unprecedented high average intrinsic quality factor (Q0) of 3.8×1010 at 16 MV/m and 3.6×1010 at 21 MV/m in the horizontal test. The cryomodule can operate stably up to a total continuous wave rf voltage greater than 193 MV, with an average cavity usable accelerating gradient of more than 23 MV/m. The results significantly exceed the specifications of Circular Electron Positron Collider and Dalian advanced light source and the other high repetition rate free electron laser facilities [Linac Coherent Light Source II (LCLS-II), LCLS-II-high energy, Shanghai High Repetition Rate X-ray FEL and Extreme Light Facility, Shenzhen Superconducting Soft X-Ray Free Electron Laser, etc.]. There is evidence that the mid-T bake cavity may not require fast cooldown or long processing time in the cryomodule. This paper reviews the cryomodule performance and discusses some important issues in cryomodule assembly and testing.

关键词： Radio frequency techniques Superconducting RF

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Medium-temperature furnace baking of 1.3 GHz 9-cell superconducting cavities at IHEP

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SUPERCONDUCTOR SCIENCE & TECHNOLOGY 2021年第9期34卷 095005-095005页

作者： He, Feisi Pan, Weimin Sha, Peng Zhai, Jiyuan Mi, zhenghui Dai, xuwen Jin, Song Zhang, Zhanjun Dong, Chao Liu, Baiqi Zhao, Hui Ge, Rui Zhao, Jianbing Mu, Zhihui Du, Lei Sun, Liangrui Zhang, Liang Yang, Conglai zheng, Xiaobing Chinese Acad Sci Inst High Energy Phys Beijing 100049 Peoples R China Chinese Acad Sci Inst High Energy Phys Key Lab Particle Accelerat Phys & Technol Beijing 100049 Peoples R China Chinese Acad Sci Ctr Superconducting RF & Cryogen Inst High Energy Phys Beijing 100049 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China

Recently, heat treatment in the range of 250 degrees C-500 degrees C has been attempted with the aim of improving the quality factor (Q) of superconducting radio-frequency (SRF) cavities worldwide. Herein, medium-temperature (mid-T) furnace baking experiments were conducted at the Institute of High Energy Physics to obtain higher Q values. First, 1.3 GHz 1-cell cavities received mid-T furnace baking at different temperatures, resulting in not only the improvement of Q but also an anti-Q-slope behavior. Furthermore, mid-T furnace baking was applied to six 1.3 GHz 9-cell cavities, all of which indicated higher Q and anti-Q-slope behavior. The average Q was 3.8 x 10(10) at 16 MV m(-1). The maximum gradients of the 9-cell cavities were 22.7-26.5 MV m(-1). Finally, the mid-T furnace baked 1.3 GHz 9-cell cavities were welded with helium vessel, with no degradation in the vertical tests. In addition, the mid-T furnace baking process was simplified in comparison with European X-ray Free Electron Laser and Linear Coherent Light Source because of the cancellation of light electro-polishing. This simplification is beneficial in the mass production of SRF cavities with high Q, which are widely adopted in free electron lasers and high-energy colliders.

关键词： superconducting cavity quality factor gradient medium-temperature furnace baking vertical test

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Stress and optical constants in thin films of BaF₂-PrF₃: A potential Th-free infrared low-index evaporation material

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OPTIK 2018年 173卷 174-179页

作者： Lva, xuwen zheng, Weimin Xie, Ping Li, Bin He, Weixiang Jing, Chao Liu, Dingquan Shanghai Univ Dept Phys Shanghai 200444 Peoples R China Shandong Univ Sch Space Sci & Phys Weihai 264209 Peoples R China Chinese Acad Sci Shanghai Inst Tech Phys Shanghai 200083 Peoples R China Shanghai Tech Univ Sch Phys Sci & Technol Shanghai 200031 Peoples R China

Although PrF3 can be regarded as a potential infrared low-index evaporation material used in infrared antireflection to substitute for radioactive ThF4 because its thin films have a good transparency, the lower refractive index n and extinction coefficient k in the spectral range of thermal infrared, the greater tensile stress emerging in its layers will deteriorate the reliability and durability of antireflection coatings. In our investigation, BaF2-PrF3 thin films were deposited using electron beam evaporation from the sintered pellets of PrF3 admixed with BaF2. Stress in thin films was calculated from Stoney's equation on the basis of measuring the changes of radius of curvature of thin silicon strips. The stoichiometry of thin films was determined using the energy dispersive X-ray analysis (EDX). The optical constants of thin films were determined by fitting the measured spectral transmittance curves using Lorentz oscillators as a dispersion model. It can be observed that stress in BaF2-PrF3 thin films is obviously reduced with the increasing of the concentration of BaF2 in thin films and the sintered pellets. Moreover, it was also demonstrated that incorporation of BaF2 into PrF3 can reduce the refractive index of thin films, although BaF2-PrF3 thin films have a greater dispersion than that of PrF3 thin films. In addition, the lower value of extinction coefficient is also presented in thin films, like those of rare-earth fluorides.

关键词： Antireflection coatings Infrared Praseodymium fluoride Barium fluoride Electron beam evaporation Stress Optical constant

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CTA1-DD蛋白在枯草芽胞杆菌中的分泌表达

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畜牧与兽医 2023年第9期55卷 42-46页

作者：张元鹏侯立婷杜露平于晓明李兰杨利张浩明王义伟乔绪稳程海卫秦竹陈瑾郑其升江苏省农业科学院动物免疫工程研究所江苏南京210014 江苏省农业科学院国家兽用生物制品工程技术研究中心江苏南京210014 江苏省动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009 江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地江苏南京210014 兽用生物制品(泰州)国泰技术创新中心江苏泰州1225300

有研究证实,霍乱毒素(CT)A1亚基与葡萄球菌G蛋白的D肽偶联构建的融合蛋白CTA1-DD具有良好的佐剂效果。为了获得重组CTA1-DD蛋白在枯草芽胞杆菌中的表达,将CTA1-DD基因连接到表达载体pHT43中,构建重组枯草杆菌表达载体pHT43-CTA1-DD。将... 详细信息

有研究证实,霍乱毒素(CT)A1亚基与葡萄球菌G蛋白的D肽偶联构建的融合蛋白CTA1-DD具有良好的佐剂效果。为了获得重组CTA1-DD蛋白在枯草芽胞杆菌中的表达,将CTA1-DD基因连接到表达载体pHT43中,构建重组枯草杆菌表达载体pHT43-CTA1-DD。将重组载体转化到枯草芽胞杆菌WB600中,用IPTG诱导重组菌表达,发酵液离心后取上清液,通过SDS-PAGE和Western blot方法分析蛋白表达情况。发酵上清液经Ni-IDA琼脂糖树脂纯化,与流感病毒HA抗原混合后共同滴鼻免疫小鼠后采集小鼠血清和支气管肺泡灌洗液检测IgG和IgA的抗体水平。结果显示:重组CTA1-DD蛋白在枯草芽胞杆菌中能够分泌表达,在发酵罐中发酵36 h情况下蛋白表达量最高,目的蛋白大小在42 kDa左右,蛋白产量最高能到700μg/mL,Western blot检测目的蛋白正确表达。重组蛋白经过Ni-IDA琼脂亲和层析纯化后与HA抗原混和滴鼻免疫小鼠,ELISA结果显示CTA1-DD蛋白组针对HA抗原能够诱导更高的血清IgG和黏膜IgA抗体水平,说明枯草芽胞杆菌表达的CTA1-DD蛋白具有佐剂活性。试验结果为进一步研究CTA1-DD蛋白在黏膜佐剂的大规模生产应用奠定了基础。

关键词： CTA1-DD蛋白枯草芽胞杆菌表达黏膜免疫

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“危急事态”和基本权利限制

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湖南工业大学学报（社会科学版） 2016年第1期21卷 50-56页

作者：郑旭文福州大学法学院福建福州350108

国家在处于危急情势或存亡威胁时,可以通过限制基本权利来消除危机。但是,为了防止国家滥用危急事态权能权能,进而侵害人民基本权利,各国设计了各项程序性机制来控制危急事态可能的失范。具言之,国家采取的各项危急措施必须遵循基本权... 详细信息

国家在处于危急情势或存亡威胁时,可以通过限制基本权利来消除危机。但是,为了防止国家滥用危急事态权能权能,进而侵害人民基本权利,各国设计了各项程序性机制来控制危急事态可能的失范。具言之,国家采取的各项危急措施必须遵循基本权利保护的最低标准,以确保在任何时候人的尊严和价值都能得以维系和实现。此外,国家危急事态措施还须遵循正当程序,接受基本权利本质内容不受侵害等原则、比例原则和法律明确性原则的检验。

关键词：危急事态基本权利限制最低标准比例原则

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Metabolomic study of natrin-induced apoptosis in SMMC-7721 hepatocellular carcinoma cells by ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry

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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2019年第0期124卷 1264-1273页

作者： Lu, Shiyin Lu, Rigang Song, Hui Wu, Jinxia Liu, xuwen Zhou, Xiaoling Yang, Jianqing Zhang, Hongye Tang, Chaoling Guo, Hongwei Hu, Jian Mao, Guifu Lin, Hanmei Su, Zhiheng zheng, Hua Guangxi Med Univ Pharmaceut Coll Nanning Peoples R China Liuzhou Tradit Chinese Med Hosp Dept Pharm Liuzhou Peoples R China Guangxi Inst Food & Drug Control Nanning Peoples R China Liuzhou Tradit Chinese Med Hosp Dept Gastroenterol Liuzhou Peoples R China Guangxi Tradit Chinese Med Univ Affiliated Hosp 1 Gynaecol Nanning Peoples R China Guangxi Med Univ Med Sci Res Ctr Nanning Peoples R China

Natrin, a new member of the cysteine-rich secretory protein (CRISP) family purified from the snake venom of Naja naja atra, has been demonstrated to have anticancer activity. However, the underlying molecular mechanisms need further elucidation. In this study, MU was used to evaluate cell viability. Apoptotic cells were analyzed by employing a transmission electron microscope (TEM). Metabolomic study of the metabolic perturbations caused by natrin-induced apoptosis in differentiated SMMC-7721 cells was performed for the first time by using integrative ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q/IDF MS). To investigate the possible mechanism in the mitochondria] pathway of natrin-induced apoptosis, we measured apoptosis-related mRNA changes using real-time fluorescent quantitative PCR (FQ-PCR). Cell proliferation was significantly inhibited after treatment with natrin in a dose dependent manner. Principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA) clearly demonstrated that metabolic profiles were affected by natrin. The results of multivariate statistical analysis showed that a total of 13 metabolites were characterized as potential biomarkers highly implicated in natrin-induced apoptosis, which corresponded to fluctuations of five pathways, including sphingolipid metabolism, fatty acid biosynthesis, fatty acid metabolism, glycerophospholipid metabolism and glycosphingolipid biosynthesis. Furthermore, natrin-induced apoptosis showed an increase in the Bax/Bcl-2 ratio in the mitochondrial pathway compared with controls. This study illustrated that rapid and holistic cell metabolomics combining molecular biological approaches might be a powerful tool for evaluating the underlying mechanisms of natrin-induced apoptosis, which would help to deepen specific insights into the anti-hepatoma mechanisms of natrin and facilitate the clinical application of natrin in the future.

关键词： Metabolomics Natrin Hepatocellular carcinoma (HCC) Apoptosis UPLC-QtrOF MS

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虎杖对水质和微生物群落结构及功能的影响

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环境科学与技术 2020年第5期43卷 25-32页

作者：陈家长赵志祥施羽露史磊磊郑尧邴旭文中国水产科学研究院淡水渔业研究中心农业部长江下游渔业生态环境监测中心农业部水产品质量安全环境因子风险评估实验室(无锡)江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214081

为研究虎杖种植后池塘水质变化和水体微生物群落碳源利用能力,该研究于室外构建了吉富罗非鱼养殖池塘5%虎杖生态浮床,2017年6-9月持续测定水质,利用Biolog技术分析池塘水体微生物群落结构及功能特征。结果表明6-7月生长期间虎杖种植能... 详细信息

为研究虎杖种植后池塘水质变化和水体微生物群落碳源利用能力,该研究于室外构建了吉富罗非鱼养殖池塘5%虎杖生态浮床,2017年6-9月持续测定水质,利用Biolog技术分析池塘水体微生物群落结构及功能特征。结果表明6-7月生长期间虎杖种植能有效削减池塘水体中TN、TP、Chl.a浓度,在温度较高的8、9月虎杖种植塘TP显著降低(P<0.05);7-9月虎杖种植塘水体微生物AWCD值显著增加(P<0.05),多样性McIntosh和Richness(除9月)指数均显著提高(P<0.05),且水体微生物对聚合糖类、氨基酸类、醇类的代谢能力显著提高(P<0.05)。在吉富罗非鱼养殖池塘种植虎杖,可在改善水质的基础上提高水体微生物群落多样性指数及对碳源的利用能力。

关键词：罗非鱼虎杖浮床微生物群落多样性碳源利用

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