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The immunopotentiator CVC1302 enhances immune efficacy and protective ability of foot-and-mouth disease virus vaccine in pigs

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VACCINE 2018年第52期36卷 7929-7935页

作者： Chen, Jin Yu, Xiaoming zheng, Qisheng Hou, Liting Du, Luping Zhang, Yuanpeng Qiao, xuwen Hou, Jibo Huang, Kehe Nanjing Agr Univ Coll Vet Med Nanjing 210095 Jiangsu Peoples R China Nanjing Agr Univ China Inst Nutr & Metab Disorders Domest Anim & F Nanjing 210095 Jiangsu Peoples R China Jiangsu Acad Agr Sci Inst Vet Immunol & Engn Nanjing 210014 Jiangsu Peoples R China Jiangsu Coinnovat Ctr Prevent & Control Important Yangzhou 225009 Jiangsu Peoples R China

The immunological enhancement characteristics of the immunopotentiator CVC1302 were evaluated for foot-and-mouth disease virus (FMDV) inactivated vaccine in pigs. Eight-week-old piglets were vaccinated with the foot-and-mouth disease (FMD) vaccine alone (FMD-vaccine group) or with the addition of CVC1302 (FMD-CVC1302 group), and the serum liquid phase blocking ELISA (LPB-ELISA) antibody titers, IgG1 and IgG2 levels, and the levels of four cytokines secreted by peripheral blood lymphocytes were measured at 28 days post vaccination (dpv). In the FMD-CVC1302 group, the LPB-ELISA antibody titers, IgG1, and IgG2 titers, and IL-2, IL-4, IL-6, and IFN-gamma levels at 28 dpv were significantly higher than those in the FMD-vaccine group. The FMD-CVC1302 group had long-lasting antibody titers (>7.8 log(2)), lasting for at least 6 months. In addition, piglets were vaccinated with or without addition of CVC1302 to the FMD vaccine at three different doses (1, 1/3, and 1/9 of the standard vaccine dose) and the serum LPBELISA antibody and serum neutralizing (SN) antibody titers were detected at 28 dpv. Then all pigs were challenged with virulent FMDV for PD50 value, and the levels of FMDV-specific RNA copies for the two full-dose groups at 3 and 10 days post challenge (dpc) were measured. The LPB-ELISA and SN antibody titers for the three doses in the FMD-CVC1302 groups were significantly higher than those in the FMD-vaccine groups at the same doses (p < 0.05). Post-virus challenge, the FMDV-specific RNA copy number in the FMD-CVC1302 group was lower than that in the FMD-vaccine group at 3 and 10 dpc. The PD50 value was 15.85 for the FMD-CVC1302 group, which was obviously higher than that for the FMD-vaccine group (10.96), and in the 1/9-dose of FMD-vaccine group only 3/5 pigs were protected. These results indicate that CVC1302 can enhance the immune efficacy and protective ability of the FMD vaccine in pigs. (C) 2018 Elsevier Ltd. All rights reserved.

关键词： Foot-and-mouth disease virus Inactivated vaccine Immunopotentiator CVC1302 Immune efficacy

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Fabrication of CoNPs-embedded porous carbon composites based on morphochemical imprinting strategy for detection of H2O2 released from living cells

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ELECTROCHIMICA ACTA 2019年 321卷

作者： Lu, Nannan zheng, Bo Gu, Yue Yan, Xiaoyi Zhang, Tingting Liu, He Xu, Haixin Xu, Zhiqian Li, xuwen Zhang, Zhiquan Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China Weinan Normal Univ Coll Chem & Mat Weinan City 714099 Peoples R China

Rational construction of biosensor to determine reactive oxygen species (ROS) generated from living cells is one of the great challenges in physiological and pathological fields. Herein, for the first time, a novel Co nanoparticles (CoNPs) embedded walnut-like N-doped porous carbon microsphere (Co@NCS) can be synthesized using dual imprinting strategy by pyrolysis and acid leaching. The distinct morphology and chemical characteristic of melamine cyanurate (MC) aggregates can be imprinted in the carbon source derived from glucose during the pyrolysis process. According to our research, with synergistic effect of CoHPs and NCS, the Co@NCS shows an excellent electrochemical activity toward the reduction of hydrogen peroxide (H2O2). The Co@NCS modified electrode exhibits a wider linear detection response with the range from 0.5 mu M to 7500 mu M and a low detection limit of 88 nM (S/N = 3). Moreover, with desirable selectivity, decent reproducibility and satisfied anti-interference performance, the fabricated biosensor can be applied to determine H2O2 in human serum samples and that released from living cell, indicating the potential applications in the fields of pathological and physiological sensing. (C) 2019 Elsevier Ltd. All rights reserved.

关键词： Co nanoparticles Porous carbon microspheres Biosensor Hydrogen peroxide Living cells

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Parasitic modes suppression in CW cold tests of 1.3 GHz 9-cell high Q cavities at IHEP

arXiv

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arXiv 2021年

作者： Mi, zhenghui He, Feisi Pan, Weimin Sha, Peng Zhai, Jiyuan Dai, xuwen Jin, Song Zhang, Zhanjun Dong, Chao Liu, Baiqi Zhao, Hui Ge, Rui Zhao, Jianbing Mu, Zhihui Du, Lei Sun, Liangrui Zhang, Liang Yang, Conglai zheng, Xiaobing Lin, Haiying Wang, Guangwei He, Xiangcong Institute of High Energy Physics Chinese Academy of Sciences Beijing100049 China Key Laboratory of Particle Acceleration Physics & Technology Institute of High Energy Physics Chinese Academy of Sciences Beijing100049 China Center for Superconducting RF and Cryogenics Institute of High Energy Physics Chinese Academy of Sciences Beijing100049 China University of Chinese Academy of Sciences Beijing100049 China

The CW RF test of 1.3 GHz 9-cell cavity in liquid helium bath at 2 K is a very important key point in the cavity procurement. Some problems can be found through the test, according which to optimized and improve the process of cavity. Recently, Medium temperature (mid-T) furnace bake of 1.3 GHz 9-cell cavities have been carried out at IHEP. Through the proceed of mid-T bake, the quality factor of cavity has been greatly improved. While the excitation of the parasitic modes in the high Q cavities CW cold test has been encountered, which implies an error source for the cavity gradient and quality factor determination. In order to ensure the testing accuracy of superconducting cavity, we have improved the testing system. Finally, the parasitic mode is completely suppressed and the CW RF cold test of high Q cavity is guaranteed. © 2021, CC BY.

关键词： Superfluid helium

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基于家庭医生签约服务的社区老年2型糖尿病患者居家护理方案构建研究

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哈尔滨医药 2021年第6期41卷 40-42页

作者：郑连花何旭文刘国莲牛萌仇艳敏买娟娟白亚茹宁夏医科大学护理学院宁夏银川750004 三二○一医院陕西汉中723000 宁夏医科大学总医院宁夏银川750004

目的在家庭医生签约服务的基础上,构建一套较为科学、实用的社区老年2型糖尿病患者居家护理方案。方法通过文献回顾、问卷调查、半结构式访谈及专家会议,确定基于家庭医生签约服务的社区老年2型糖尿病患者居家护理方案的框架及具体内容... 详细信息

目的在家庭医生签约服务的基础上,构建一套较为科学、实用的社区老年2型糖尿病患者居家护理方案。方法通过文献回顾、问卷调查、半结构式访谈及专家会议,确定基于家庭医生签约服务的社区老年2型糖尿病患者居家护理方案的框架及具体内容。结果参会专家判断依据系数为0.910,熟悉程度系数为0.820,权威系数为0.865,构建基于家庭医生签约服务的社区老年2型糖尿病患者居家护理方案内容包括居家护理方案的目标和宗旨、居家护理服务团队、居家护理服务流程及内容三部分。结论本研究构建的居家护理方案科学、合理、可靠,可为社区医疗机构开展老年2型糖尿病患者居家护理服务提供依据。

关键词：家庭医生签约服务老年人 2型糖尿病居家护理方案专家会议法

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Gut microbiota analysis of juvenile genetically improved farmed tilapia (Oreochromis niloticus) by dietary supplementation of different resveratrol concentrations

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FISH & SHELLFISH IMMUNOLOGY 2018年 77卷 200-207页

作者： zheng, Yao Wu, Wei Hu, Gengdong Qiu, Liping Meng, Shunlong Song, Chao Fan, Limin Zhao, Zhixiang Bing, xuwen Chen, Jiazhang Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr 9 Shanshui East Rd Wuxi 214081 Jiangsu Peoples R China Minist Agr Fishery Ecoevironm Monitoring Ctr Lower Reaches Y Wuxi 214081 Jiangsu Peoples R China Minist Agr Lab Qual & Safety Risk Assessment Aquat Prod Envi Wuxi 214081 Jiangsu Peoples R China Nanjing Agr Univ Wuxi Fishery Coll Wuxi 214081 Jiangsu Peoples R China Minist Agr Key Lab Control Qual & Safety Aquat Prod Wuxi Jiangsu Peoples R China

The genetically improved farmed tilapia (GIFT, Oreochromis niloticus) is cultured widely for production of freshwater fish in China, while streptococcosis, likely related to pathogenic infections, occurs frequently in juvenile, mother, and operated GIFT. The gut microbiota plays an important role in nutrient digestibility in animals, and resveratrol (RES) has been used in feed for different freshwater fish species. Therefore, understanding changes in the tilapia gut microbiota across different concentrations of dietary RES supplementation is extremely important. The gut microbiota population in tilapia at 45 d after supplementation with different concentrations (0, 0.025, 0.05, 0.1 g/kg) of dietary RES was assessed by 16S rDNA gene sequencing. A total of 5445 operational taxonomic units were identified from all samples, and 14 phyla and 81 families were identified from all fecal samples. The bacteria of the phylum Firmicutes were significantly enriched in the 0.025 g/kg RES group when compared with the controls. Proteobacteria, Firmicutes and Cyanobacteria were the most dominant three phyla in all samples. With the increasing concentrations, the proportion of beneficial microbial taxa (Acetobacteraceae and Methylobacteriaceae) increased, whereas the proportion of harmful microbial taxa decreased, eg. Streptococcaceae except for 0.1 g/kg RES groups. RES did not affect the richness and diversity in tilapia gut microbiota. These findings provide information on the diversity and differences in GIFT gut microbiota database, and may contribute to developing strategies for management of diseases and long-term sustainability of O. niloticus culture.

关键词： Resveratrol GIFT Gut microbiota 16S rDNA Disease defense

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Production and Water Quality Responses in Tilapia Aquaculture Tanks Receiving Organic Fertilizers and Algae

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Agricultural Biotechnology 2019年第4期8卷 156-162页

作者： Shunlong MENG Dandan LI Liping QIU Gengdong HU Limin FAN Chao SONG Yao zheng Wei WU Jiazhang CHEN xuwen BING Freshwater Fisheries Research Center Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze RiverMinistry of Agriculture/Key Laboratory of Fishery Eco-environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze RiverChinese Academy of Fishery SciencesWuxi 214081China Wuxi Fishery College Nanjing Agricultural UniversityWuxi 214081China

A two-month trial was carried out to evaluate the possibility of using algae and animal manure in tilapia *** the effect of algae,chicken manure and algae mixture together,cattle manure and algae mixture together,chicken-cattle manure and algae mixture together on the water quality and tilapia production was *** results showed that the yield of tilapia were 3.46,4.33,3.81,2.92 and 3.76 kg in Control,Algae,Chicken-A,Cattle-A and C-C-A,respectively,following the order of Algae>Chicken-A>C-C-A>Control>Cattle-A,and tilapia yield in Algae and chicken manure treatment groups increased by 25.0%and 10.1%respectively compared with the *** and chicken manure could increase the tilapia production,but cattle manure has no the *** the effect of algae and animal manure on water quality showed that adding chicken manure into tilapia pond could make water quality decreased at the beginning 20 d,but could increase water quality after 20 d and it can stabilize the phytoplankton structure in aquaculture *** Chlorella vulgaris and Scenedesmus quadricauda into tilapia pond could make water quality in a good state during the aquaculture process and it can stabilize the phytoplankton structure in aquaculture water.

关键词： Algae Chicken manure Cattle manure Tilapia production Water quality

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Effect of Organic Fertilizer on Aquaculture Water Quality and Tilapia Production

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Agricultural Biotechnology 2019年第5期8卷 92-98页

作者： Shunlong MENG Xi Chen Dandan LI Liping QIU Gengdong HU Limin FAN Chao SONG Yao zheng Wei WU Jiazhang CHEN xuwen BING Freshwater Fisheries Research Center Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze RiverMinistry of Agriculture/Key Laboratory of Fishery Eco-environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze RiverChinese Academy of Fishery SciencesWuxi 214081China Wuxi Fishery College Nanjing Agricultural UniversityWuxi 214081China

A two-month trial was carried out in China to evaluate the possibility of recycling animal manure through pond tilapia *** the effects of chicken manure,cattle manure and chicken-cattle mixture together on the water quality and tilapia production were *** results showed that the yield of tilapia was 3.46,3.89,2.49 and 3.20 kg in the control,chicken M,cattle M,and chicken-cattle M,respectively,and the tilapia yields following the order of chicken M>control>chicken-cattle M>cattle *** tilapia yield in chicken M group increased by 12.43% compared with the *** manure could increase the tilapia production,but cattle manure has no the *** the effect of animal manure on water quality showed that adding chicken manure into tilapia pond could make water quality decreased at the beginning 20 d,but could increase water quality after 20 d and stabilize the phytoplankton structure.

关键词： Chicken manure Cattle manure Tilapia production Water quality Aquaculture

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青藏高原积雪深度对延伸期预报技巧的影响

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大气科学 2019年第1期43卷 142-154页

作者：韩世茹郑志海周须文于长文车少静顾光芹卓嘎河北省气候中心石家庄050021 国家气候中心中国气象局气候研究开放实验室北京100081 珠海区域气候-环境-生态预测预警协同创新中心广东珠海519087 西藏自治区气候中心拉萨850000

高原积雪是重要的陆面因子,其变化的时间尺度长于大气而短于海洋。本文利用国家气候中心第二代月动力延伸期预测模式(DERF2.0)历史回报资料与被动微波资料(SMMR)、被动微波成像专用传感器(SSM/I)数据反演的逐日雪深资料,分析了1983～201... 详细信息

高原积雪是重要的陆面因子,其变化的时间尺度长于大气而短于海洋。本文利用国家气候中心第二代月动力延伸期预测模式(DERF2.0)历史回报资料与被动微波资料(SMMR)、被动微波成像专用传感器(SSM/I)数据反演的逐日雪深资料,分析了1983～2014年冬季和春季转换季节高原积雪对热带外地区延伸期尺度预测技巧的影响。结果表明,高原积雪异常年动力模式在高原积雪显著影响的青藏高原地区、贝加尔湖地区和北太平洋地区预报技巧明显高于正常年份。随着预报时效的延长,高原积雪偏多年的技巧衰减最慢、其次为积雪偏少年,积雪正常年最快,表明高原积雪异常年可预报时效更长,且高原积雪异常对预报技巧的改善在第1候的预报中就显现出来,尤其是积雪偏多年,其影响时段明显要早于海洋。结果显示高原积雪对延伸期预报技巧有重要贡献,暗示高原积雪异常为东亚延伸期预报的潜在可预报源。

关键词：青藏高原积雪深度延伸期预报模式评估

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基于Ecopath评估蟹-稻复合生态系统营养结构和能量流动

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生态学报 2020年第14期40卷 4852-4862页

作者：汪倩胡庚东宋超李丹丹郑尧裘丽萍范立明孟顺龙陈家长邴旭文中国水产科学研究院淡水渔业研究中心无锡214081 中国水产科学研究院内陆渔业生态环境和资源重点开放实验室无锡214081 农业农村部长江下游渔业生态环境监测中心无锡214081 南京农业大学无锡渔业学院无锡214081

蟹-稻综合种养模式为我国水产养殖以及农业从单一经营转变为复合经营提供了一种新模式,为了促进蟹-稻共作模式的推广和普及,应用Ecopath生态通道模型对该系统进行了营养结构和能量流动的分析。研究结果表明蟹-稻共作生态系统的最高营养... 详细信息

蟹-稻综合种养模式为我国水产养殖以及农业从单一经营转变为复合经营提供了一种新模式,为了促进蟹-稻共作模式的推广和普及,应用Ecopath生态通道模型对该系统进行了营养结构和能量流动的分析。研究结果表明蟹-稻共作生态系统的最高营养级(3.140)高于稻田单作系统的最高营养级(2.676),生态系统的能量流动主要有两条途径,即以沉水植物和其他维管束植物为起点的牧食链及以碎屑为起点的腐质链;蟹-稻共作农田生态系统在6月和10月的Finn′s循环指数(Finn′s cycling index,FCI,0.55和1)和Finn′s循环平均能流路径长度(Finn′s cycling mean path length,FCL,2.066和2.077),稻田单作农田生态系统在6月和10月的FCI(1.14和0.81)和FCL(2.089和2.137),说明蟹-稻共作模式可以形成一个稳定的生态系统,中华绒螯蟹的加入可以促进该完整的生态系统的能量流动,且随着螃蟹的成长,系统成熟度有所提高,只是较单作系统的成熟度低,共作系统有更大的成熟空间;蟹-稻共作农田生态系统在6月和10月的连接指数(Connectance index,CI)分别为0.135和0.135,稻田单作系统在6月和10月的CI为0.188和0.161,共作系统的各功能组之间的连接强度较单作系统弱,稳定程度相对较低,蟹-稻共作农田生态系统的初级生产力显著高于单作农田生态系统,且蟹-稻共作农田生态系统的发育良好。

关键词：生态通道模型蟹-稻复合生态系统营养结构能量流动

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Papillomavirus can be transmitted through the blood and produce infections in blood recipients: Evidence from two animal models

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EMERGING MICROBES & INFECTIONS 2019年第1期8卷 1108-1121页

作者： Cladel, Nancy M. Jiang, Pengfei Li, Jingwei J. Peng, xuwen Cooper, Timothy K. Majerciak, Vladimir Balogh, Karla K. Meyer, Thomas J. Brendle, Sarah A. Budgeon, Lynn R. Shearer, Debra A. Munden, Regina Cam, Maggie Vallur, Raghavan Christensen, Neil D. zheng, Zhi-Ming Hu, Jiafen Penn State Univ Coll Med Jake Gittlen Labs Canc Res Hershey PA 17033 USA Penn State Univ Coll Med Dept Pathol Hershey PA 17033 USA NCI Tumor Virus RNA Biol Sect RNA Biol Lab NIH Frederick MD 21702 USA Wenzhou Med Univ Sch Basic Med Sci Dept Immunol & Microbiol Wenzhou Peoples R China Penn State Univ Coll Med Dept Comparat Med Hershey PA 17033 USA NIAID Integrated Res Facil Ft Detrick NIH Frederick MD USA NCI CCBR Ctr Canc Res NIH Bethesda MD 20892 USA Frederick Natl Lab Canc Res Adv Biomed Computat Sci Frederick MD USA Penn State Univ Coll Med Dept Microbiol & Immunol Hershey PA 17033 USA

Human papillomaviruses (HPV) contribute to most cervical cancers and are considered to be sexually transmitted. However, papillomaviruses are often found in cancers of internal organs, including the stomach, raising the question as to how the viruses gain access to these sites. A possible connection between blood transfusion and HPV-associated disease has not received much attention. Here we show, in rabbit and mouse models, that blood infected with papillomavirus yields infections at permissive sites with detectable viral DNA, RNA transcripts, and protein products. The rabbit skin tumours induced via blood infection displayed decreased expression of SLN, TAC1, MYH8, PGAM2, and APOBEC2 and increased expression of SDRC7, KRT16, S100A9, IL36G, and FABP9, as seen in tumours induced by local infections. Furthermore, we demonstrate that blood from infected mice can transmit the infection to uninfected animals. Finally, we demonstrate the presence of papillomavirus infections and virus-induced hyperplasia in the stomach tissues of animals infected via the blood. These results indicate that blood transmission could be another route for papillomavirus infection, implying that the human blood supply, which is not screened for papillomaviruses, could be a potential source of HPV infection as well as subsequent cancers in tissues not normally associated with the viruses.

关键词： Papillomavirus blood transfusion animal models CRPV/rabbit model MmuPV1/mouse model viral infection

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