Visual tracking integrates the technology of image processing and pattern recognition, etc., which has a lot of potential applications, such as automatic driving, safety monitoring, etc. This paper analyzes the advant...
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The cholera toxin B subunit (CTB) is a nontoxic portion of the cholera toxin that retains mucosal adjuvant properties. Expression of CTB in Escherichia coli is difficult as CTB aggregates and accumulates as insoluble ...
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The cholera toxin B subunit (CTB) is a nontoxic portion of the cholera toxin that retains mucosal adjuvant properties. Expression of CTB in Escherichia coli is difficult as CTB aggregates and accumulates as insoluble inclusion bodies. To remedy this problem, the periplasmic chaperone, SKP, was investigated as possible co-expression partner to increase the solubility of recombinant CTB (rCTB) in E. coil. The result showed co-expression of SKP enhanced the soluble expression of rCTB in E. coli. Moreover, soluble rCTB was successfully expressed and secreted into the periplasmic space through the direction of the LTB leader signal. rCTB in periplasm was purified using an immobilized D-galactose resin;GM1-ELISA ex-periments showed that rCTB retains strong GM1 ganglioside-binding activity. Intranasal administration of ovalbumin (OVA) with rCTB significantly induced both mucosal and humoral immune responses specific to OVA. These data indicate that co-expression of the molecular chaperone SKP with CTB increased the solubility of rCTB while maintaining its function. (c) 2017 Elsevier Inc. All rights reserved.
Dietary resveratrol (RES) supplementation may have some pharmacological effects including anti inflammation. Previous studies have shown that Kupffer cell activation and apoptosis induction increases the transcription...
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Dietary resveratrol (RES) supplementation may have some pharmacological effects including anti inflammation. Previous studies have shown that Kupffer cell activation and apoptosis induction increases the transcription of pro- and anti-inflammatory cytokines. The main purpose of this study was to investigate the pro- and anti-inflammatory activities of 0.1 or 0.3 g/kg RES as a dietary supplement in juvenile freshwater tilapia (Oreochromis niloticus). The results showed that hepatic and serum immunoglobulin M (IgM) significantly decreased and increased while anti- and pro-inflammatory cytokines significantly increased and decreased, respectively, in the RES-treated groups. The expression of serum and hepatic IgM and anti-inflammatory cytokines [interleukin (IL)-10] and its inverse inhibitor interferon (IFN)-gamma significantly increased while pro-inflammatory cytokine transcription significantly decreased. Hematoxylin-eosin staining and scanning electron microscopy revealed intestinal deformation, irregular goblet cells, and apoptotic cells in the 03 g/kg RES groups. RES (0.3 g/kg) also induced necrosis, apoptosis, reduction in Kupffer cell number, compressed sinusoids, and deformation of epidermal cells in the liver of the treated groups. In conclusion, the results of the present study show that high doses of RES were absorbed in the gut and then damaged the liver and intestinal tissue. (C) 2017 Published by Elsevier Ltd.
Rice-fish integrated farming （RFIF） can change the agricultural production pattern from mono-farming model to the co-farming model, and the stereoscopic and integrated farming system established by RFIF can change t...
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Rice-fish integrated farming （RFIF） can change the agricultural production pattern from mono-farming model to the co-farming model, and the stereoscopic and integrated farming system established by RFIF can change the flat production to the stereoscopic production. Therefore, RFIF is the important development direction for agriculture to change pattern and adjust structure. In order to further promote development of the research and application of RFIF, the general development situation of RFIF in China was reviewed; the effects of RFIF on increasing farmer income and enhancing agricultural efficiency were clarified. The technologies matched with RFIF, such as rice paddy engineering technology, safe plant protection technology, security fertilization technology, fish disease prevention and control technology and water quality management technology, were summarized, and the supporting role of RFIF for agriculture was pointed out. In addition, the development prospect of RFIF was proposed, as well as the key research direction of RFIF.
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