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动物营养学报 2022年第1期34卷 589-599页

作者：施羽露杨晓曦郑尧裘丽萍胡庚东邴旭文陈家长南京农业大学无锡渔业学院无锡214081 中国水产科学研究院淡水渔业研究中心/农业农村部长江下游渔业生态环境监测中心/农业农村部水产品质量安全环境因子风险评估实验室无锡214081

本试验旨在探究饲料中添加白藜芦醇(Res)对高脂摄食红罗非鱼生长性能、腹脂沉积、肠道组织结构及炎症因子含量的影响。选用红罗非鱼360尾,设置8%脂肪(A组)、10%脂肪(B组)、10%脂肪+Res(C组)以及10%脂肪+Res+抑制剂EX527(D组)4个组,每组... 详细信息

本试验旨在探究饲料中添加白藜芦醇(Res)对高脂摄食红罗非鱼生长性能、腹脂沉积、肠道组织结构及炎症因子含量的影响。选用红罗非鱼360尾,设置8%脂肪(A组)、10%脂肪(B组)、10%脂肪+Res(C组)以及10%脂肪+Res+抑制剂EX527(D组)4个组,每组3个重复,每个重复30尾,试验期为63 d。分别在第3、6、9周测定末体重,并采集中肠组织、肠道内容物和血清,测定炎症因子及脂多糖(LPS)含量。结果表明:与A组相比,B组体重、增重率和特定生长率显著升高(P<0.05),且第3、6周腹脂率显著升高(P<0.05),第9周中肠组织白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量显著升高(P<0.05),中肠组织转化生长因子-β(TGF-β)含量显著降低(P<0.05);血清LPS含量显著升高(P<0.05),肠道内容物LPS含量随时间呈上升趋势。与B组相比,C组体重、增重率、特定生长率、饲料系数显著降低(P<0.05);血清LPS含量显著降低(P<0.05),且肠道内容物LPS含量随时间呈下降趋势;第9周中肠组织IL-1β、白细胞介素-6(IL-6)和TNF-α含量显著降低(P<0.05),TGF-β含量显著升高(P<0.05)。B组肠道环状皱襞均存在破损现象,C组肠道杯状细胞较B组和D组有所增加。综上所述,添加Res可通过降低LPS含量、提高杯状细胞数量缓解因高脂饲料导致的腹腔脂肪过度沉积、肠道炎症及组织病变,以调控鱼生长,有效保护肠道健康。

关键词：白藜芦醇红罗非鱼肠道炎症因子肠道显微结构

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Varieties of immunity activities and gut contents in tilapia with seasonal changes

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FISH & SHELLFISH IMMUNOLOGY 2019年 90卷 466-476页

作者： zheng, Yao Wu, Wei Hu, Gengdong Qiu, Liping Bing, xuwen Chen, Jiazhang Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr 9 Shanshui East Rd Wuxi 214081 Jiangsu Peoples R China

We performed 16S rDNA sequencing of tilapia fecal samples to analyze changes in tilapia gut contents after cultivation of the fish in the presence of sandwich-like floating beds of Chinese medicinal herbs (5 and 10% planting-areas;5% Polygonum cuspidatum). The interactive effects between water quality and blood and hepatic pro- and anti-inflammatory concentrations were also assessed. Our results showed that the water quality (i.e., NO3--N, NO2--N, TP removal rates) improved, and the abundance of Chloroflexi and Cyanobacteria increased. The abundance of Bacteroidetes, Verrucomicrobia, Saccharibacteria, and Actinobacteria showed both significant seasonal decreases and increases in the presence of P. cuspidatum (increases in August and decreases in July). Fish blood and hepatic IL-10 and IFN-gamma levels (together with fish sampled in September) significantly increased in the P. cuspidatum group sampled in August, while those of TNF-alpha (10% sandwich-like, P. cuspidatum), IL-1 beta (P. cuspidatum), IL-8 (5% sandwich-like in September, S905S) significantly decreased. Heat shock proteins 60 and 70 levels significantly increased in the P. cuspidatum group, and complement C3 and C4 concentrations significantly increased in S905S. This study demonstrated that enhanced immunity through the regulation of pro- and anti-inflammatory proteins was sustained throughout development until harvest, particularly in fish grown with P. cuspidation.

关键词： Tilapia Gut contents Floating bed cultivation 16S rDNA Disease

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基于横波检测柱状材料内部缺陷的数值模拟研究

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应用激光 2022年第5期42卷 109-119页

作者：崔缙郭华玲郑宾刘辉张钰龙邓祎昕贺旭文中北大学电气与控制工程学院山西太原030051

小口径金属圆柱体的缺陷检测是无损检测领域的一个难题。通过有限元方法对金属圆柱中横波传播特性以及与缺陷作用机理进行数值模拟。探究了横波的方向性变化、圆孔缺陷半径对横波传播影响、不同位置和不同长度的缺陷对横波传播的影响。... 详细信息

小口径金属圆柱体的缺陷检测是无损检测领域的一个难题。通过有限元方法对金属圆柱中横波传播特性以及与缺陷作用机理进行数值模拟。探究了横波的方向性变化、圆孔缺陷半径对横波传播影响、不同位置和不同长度的缺陷对横波传播的影响。研究表明横波在传播过程中主要能量范围由15°~36°变为20°~35°,提出了近似正三角形的传播路径并进行验证,发现横波到达表面后发生模态转换,一部分声波以略面纵波的速度沿表面传播;对于圆孔缺陷,圆孔半径在3.4~4.6mm范围内可通过幅值变化判断孔径信息,半径在4.6~5.8mm范围内可通过接收时间判断孔径信息;对于不同位置的矩形缺陷,当缺陷进入到横波主要能量范围内,C扫图中出现近似线性的高能量区域,探测角度随着缺陷位置增加而增大,此范围内的缺陷可通过反射信号的探测角度判断位置信息;对于不同长度的矩形缺陷,随着缺陷长度增加,透射波能量减弱并且探测角度减小,呈现出近似线性的能量分布,通过探测透射波到达外表面的角度可判断缺陷长度信息。提出了相应的传播路径和长度计算公式,计算结果得到的误差百分比控制在6.5%以内,验证了该缺陷长度检测方法的有效性。

关键词：横波柱状材料激光超声内部缺陷数值模拟

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Reshaping fecal gut microbiota composition by growing with Polygonum cuspidatum, Houttuynia cordata, and Ipomoea aquatica

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CANADIAN JOURNAL OF MICROBIOLOGY 2019年第7期65卷 522-529页

作者： zheng, Yao Hu, Gengdong Wu, Wei Qiu, Liping Li, Dandan Bing, xuwen Chen, Jiazhang Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Wuxi Jiangsu Peoples R China

We carried out sequencing of samples cultivated in floating beds with different Chinese medicinal herbs (Control, Houttuynia cordata Thunb., Polygonum cuspidatum, and a combination of H. cordata with Ipomoea aquatica Forssk.;named groups A, B, C, D, respectively) to analyze changes in the composition of gut microbiota of tilapia feces. Fusobacteria (ranging from 49.0% to 73.3%), Firmicutes (12.3%-37.8%), and Proteobacteria (5.1%-23.0%) were found to be the most dominant phyla present in all samples. The operational taxonomic units and the Ace and Chao1 indices of groups A and D were significantly higher than those of group C. Polygonum cuspidatum decreased the species richness and diversity of microbial communities in tilapia intestinal feces. The phylum WCHB1-60, order Enterobacteriales, and genus Plesiomonas significantly decreased (in group A);the species Plesiomonas shigelloides significantly decreased (in groups B and C);and the genus Leucobacter significantly increased (in group D) when compared with the control. The relative abundance of the class Verrucomicrobiae (groups B vs C) significantly decreased. In the presence of I. aquatica, the phylum Bacteroidetes significantly decreased, while the genera Leucobacter and Pelotomaculum significantly increased. The ratio of Bacteroidetes to Firmicutes was significantly higher in groups B and C relative to the controls, while it decreased significantly in group D. The algae (i. e., Anabaena and Microcystis) and beneficial pathogenic bacteria decreased in groups C and D, respectively. In addition, Enterovibrio decreased in all treatment groups. The present data demonstrate that floating bed cultivation with Chinese medicinal herbs significantly alters the gut microbiota of tilapia, which may enhance its immune activity.

关键词： tilapia fecal gut microbiota floating bed cultivation 16S rDNA disease

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再生骨料混凝土结构抗震设计关键问题

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土木工程学报 2020年第11期53卷 46-54页

作者：肖建庄王春晖郑振鹏肖绪文同济大学上海200092 同济大学上海200092 上海市城市建设设计研究总院(集团)有限公司上海200125 同济大学上海200092

随着砂石骨料日益短缺、价格上涨,以及国家对生态环保事业的日益重视,在结构工程中使用再生骨料混凝土(简称为“再生混凝土”)的时机已经基本成熟.然而,在实际工程应用中,结构设计人员对再生混凝土结构设计关键参数的选取方法不明确,大... 详细信息

随着砂石骨料日益短缺、价格上涨,以及国家对生态环保事业的日益重视,在结构工程中使用再生骨料混凝土(简称为“再生混凝土”)的时机已经基本成熟.然而,在实际工程应用中,结构设计人员对再生混凝土结构设计关键参数的选取方法不明确,大多沿用普通混凝土结构的设计参数,这给再生混凝土结构的应用埋下了安全隐患.文章基于将再生混凝土应用于上海12层框架-剪力墙结构设计中遇到的诸多问题及解决方法,结合近年来对再生混凝土结构抗震性能的试验研究和现场监测结果进行深入分析和讨论,旨在提出安全、可靠的再生混凝土结构设计及非线性分析过程中的关键参数,包括力学参数、本构模型、阻尼比参数等输入参数,以及轴压比限值和层间位移角限值等控制指标,并讨论了设计软件的选取,为推广再生混凝土结构抗震设计技术提供理论和实践依据.

关键词：再生骨料混凝土结构抗震设计力学参数本构模型阻尼比参数轴压比层间位移角

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Development of GEM-PA-nanotrap for purification of foot-and-mouth disease virus

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VACCINE 2019年第24期37卷 3205-3213页

作者： Cheng, Haiwei Chen, Jin Cai, Zizheng Du, Luping Hou, Jibo Qiao, xuwen zheng, Qisheng Jiangsu Acad Agr Sci Inst Vet Immunol & Engn Nanjing 210014 Jiangsu Peoples R China Jiangsu Acad Agr Sci Key Lab Vet Biol Engn & Technol Minist Agr Natl Res Ctr Engn & Technol Vet Biol Nanjing 210014 Jiangsu Peoples R China Jiangsu Coinnovat Ctr Prevent & Control Important Yangzhou 225009 Jiangsu Peoples R China Nanjing Agr Univ Nanjing 210095 Jiangsu Peoples R China

Vaccination is the primary preventative measure against outbreaks of foot-and-mouth disease (FMD). The efficacy of inactivated FMD vaccines is mainly determined by the integrity of foot-and-mouth disease virus (FMDV) particles (referred to as 146S particles), and impurities in the inactivated vaccines could result in side effects. In this study, we developed an effective affinity purification method for the purification of FMDV from cellular lysates, referred to as GEM-PA-nanotrap. To develop the GEM-PA-nanotrap, a nanobody (Nb205) against FMDV vaccine strain O/MYA98/BY/2010 146S particles was selected from a non-immunized library and fused to a peptidoglycan-binding protein anchor (PA). The PA-Nb205 fusion protein was non-covalently coupled to the surface of Gram-positive enhancer matrix (GEM) particles, which were prepared from the non-living, non-genetically modified, Gram-positive, food-grade Lactococcus lactis bacteria. The GEM-PA-nanotrap was used to purify FMDV from cellular lysates through a simple incubation and centrifugation step. The FMDV recovery rate was more than 99%, the efficiency of nonviral protein removal was about 98.3%, and the purification process had almost no effect on the integrity and immunogenicity of 146S particles. Therefore, the GEM-PA-nanotrap has potential as an effective method for the recovery and purification of FMDV during the vaccine manufacturing process. (C) 2019 Elsevier Ltd. All rights reserved.

关键词： FMDV Affinity purification Gram-positive enhancer matrix particles Nanobody Nanotrap

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In-Situ Transformed Ni, S-Codoped CoO from Amorphous Co-Ni Sulfide as an Efficient Electrocatalyst for Hydrogen Evolution in Alkaline Media

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ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2019年第14期7卷 12501-12509页

作者： Lu, Wangting Li, xuwen Wei, Feng Cheng, Kun Li, Wenhui Zhou, Youhua zheng, Wanquan Pan, Lei Zhang, Geng Jianghan Univ Inst Interdisciplinary Res 8 Sanjiaohu Rd Wuhan 430056 Hubei Peoples R China Jianghan Univ Sch Chem & Environm Engn 8 Sanjiaohu Rd Wuhan 430056 Hubei Peoples R China Univ Paris Saclay Univ Paris Sud CNRS Inst Sci Mol Orsay F-91405 Orsay France Jianghan Univ Sch Life Sci 8 Sanjiaohu Rd Wuhan 430056 Hubei Peoples R China Huazhong Agr Univ Coll Sci Dept Chem 1 Shizishan St Wuhan 430070 Peoples R China

Amorphous materials have recently received much attention as catalysts for electrochemical reactions. However, there are relatively few reports focused on the structure change of amorphous catalysts during the catalysis process, although this is quite important to the identification of active species and in revealing the reaction mechanism. Herein, amorphous Co-Ni sulfide in the form of a nanoplate and nanowire hybrid on the surface of nickel foam is prepared as the electrode for the hydrogen evolution reaction (HER). During the hydrogen evolution process, the amorphous Co-Ni sulfide is in-situ transformed into crystalline Ni, S-co-doped CoO which is actually responsible for the catalysis of HER The transformation process is studied in detail by high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), and theoretical calculations. The in-situ formed electrode presents a high activity and durability toward HER in alkaline media. The Ni and S dopants, sulfurization temperature, and electrode substrate are revealed to be important factors that affect the activity. The present work will not only give more insights in the structure evolution of the electrocatalyst during HER but also provides a new strategy to the fabrication of new electrocatalysts.

关键词： Water splitting HER Amorphous material Oxide Doping

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Fast sulfurization of nickel foam-supported nickel-cobalt carbonate hydroxide nanowire array at room temperature for hydrogen evolution electrocatalysis

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ELECTROCHIMICA ACTA 2019年 318卷 252-261页

作者： Lu, Wangting Li, xuwen Wei, Feng Cheng, Kun Li, Wenhui Zhou, Youhua zheng, Wanquan Pan, Lei Zhang, Geng Jianghan Univ Inst Interdisciplinary Res Wuhan 430056 Hubei Peoples R China Jianghan Univ Sch Chem & Environm Engn Wuhan 430056 Hubei Peoples R China Univ Paris Saclay Univ Paris Sud Inst Sci Mol Orsay CNRS F-91405 Orsay France Jianghan Univ Sch Life Sci Wuhan 430056 Hubei Peoples R China Huazhong Agr Univ Coll Sci Dept Chem Wuhan 430070 Hubei Peoples R China

Transition metal sulfide is considered as a kind of promising noble metal-free material for hydrogen evolution reaction (HER). However, most of conventional sulfurization methods require high temperature and/or long reaction time. Herein, a sulfurization strategy at room temperature (25 degrees C) by using Na2S in aqueous solution is applied to sulfurize nickel foam-supported nickel-cobalt carbonate hydroxide nanowire array, during which amorphous sulfide gradually forms on the surface of nanowire and finally replaces the nanowire completely. It is found that the electrode sulfurized for 5 min in which only a thin sulfide layer is formed presents nearly identical catalytic performance for HER with the electrode that is sufficiently sulfurized for 120 min. The electrode sulfurized at 25 degrees C for 5 min only needs an overpotential of 100 mV to achieve hydrogen evolution current at 10 mA cm(-2) in 1 M KOH, and negligible loss of activity is observed after working at a current density of 20 mA cm(-2) for 25 h. The interface between carbonate hydroxide and sulfide is considered to play an important role in the catalysis of HER. The observation in this work can provide new insights into the sulfurization process and the role of sulfide in the process of HER. (C) 2019 Elsevier Ltd. All rights reserved.

关键词： HER Sulfide Amorphous Interface DFT

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Smart hypoxia-responsive transformable and charge-reversible nanoparticles for the deep penetration and tumor microenvironment modulation of pancreatic cancer

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BIOMATERIALS 2022年第0期287卷 000-000页

作者： Chen, Hongyi Guo, Qin Chu, Yongchao Li, Chao Zhang, Yiwen Liu, Peixin Zhao, Zhenhao Wang, Yu Luo, Yifan Zhou, zheng Zhang, Tongyu Song, Haolin Li, xuwen Li, Chufeng Su, Boyu You, Haoyu Sun, Tao Jiang, Chen Fudan Univ Key Lab Smart Drug Delivery State Key Lab Med Neurobiol Dept PharmaceutSch PharmInst Brain SciMinist Ed Shanghai 201203 Peoples R China Fudan Univ Inst Brain Sci MOE Frontiers Ctr Brain Sci Sch PharmDept Pharmaceut Shanghai 201203 Peoples R China

The compact extracellular matrix (ECM) of pancreatic ductal adenocarcinoma (PDAC) is the major physical barrier that hinders the delivery of anti-tumor drugs, leading to strong inherent chemotherapy resistance as well as establishing an immunosuppressive tumor microenvironment (TME). However, forcibly destroying the stroma barrier would break the balance of delicate signal transduction and dependence between tumor cells and matrix components. Uncontrollable growth and metastasis would occur, making PDAC more difficult to control. Hence, we design and construct an aptamer-decorated hypoxia-responsive nanoparticle s(DGL)(n)@Apt co-loading gemcitabine monophosphate and STAT3 inhibitor HJC0152. This nanoparticle can reverse its surficial charge in the TME, and reduce the size triggered by hypoxia. The released ultra-small DGL particles loading gemcitabine monophosphate exhibit excellent deep-tumor penetration, chemotherapy drugs endocytosis promotion, and autophagy induction ability. Meanwhile, HJC0152 inhibits overactivated STAT3 in both tumor cells and tumor stroma, softens the stroma barrier, and reeducates the TME into an immune-activated state. This smart code -livery strategy provides an inspiring opportunity in PDAC treatment.

关键词： Hypoxia-responsive Sizereduction Chargereversal Deeppenetration TMEmodulation

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Development of haemagglutination assay for titration of porcine circovirus type 2

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ANALYTICAL BIOCHEMISTRY 2020年 598卷 113706-000页

作者： Cheng, Haiwei Yang, Li Cai, Zizheng Qiao, xuwen Du, Luping Hou, Jibo Chen, Jin zheng, Qisheng Jiangsu Acad Agr Sci Inst Vet Immunol & Engn Nanjing 210014 Peoples R China Jiangsu Acad Agr Sci Natl Res Ctr Engn & Technol Vet Biol Key Lab Vet Biol Engn & Technol Minist Agr Nanjing 210014 Peoples R China Jiangsu Coinnovat Ctr Prevent & Control Important Yangzhou 225009 Jiangsu Peoples R China Minist Sci & Technol Jiangsu Key Lab Food Qual & Safety State Key Lab Cultivat Base Beijing Peoples R China Nanjing Agr Univ Coll Vet Med Nanjing 210095 Peoples R China Nanjing Agr Univ Nanjing 210095 Peoples R China

Porcine circovirus type 2 (PCV2) was one of the most economically important viral pathogens in all the swineproducing countries and often resulted in tremendous economic losses for the swine industry. As PCV2 could not cause cytopathogenic effects while propagated in infected cells, many complicated experiments should be performed to titrate its virus titer. In this study we developed a simple and effective hemagglutination assay for titration of virus titer of PCV2. To develop the hemagglutination assay, a recombinant bispecific nanobody (BsNb) against PCV2 and chicken red blood cells (cRBCs) was constructed based on two nanobodies (NbPCV11 and NbRBC48) which were selected from the non-immunized nanobody library, respectively. The hemagglutination assay was used to titrate the virus titer of PCV2 propagated in cell culture by simple naked-eye observation within 30 min, with the detection limit of 10(4.09) tissue culture infective dose 50 (TCID50)/mL, excellent specificity and reproducibility. Therefore, the hemagglutination assay had potential to be a rapid, reliable, cost-effective, user-friendly qualitative and semi-quantitative tool for titration of virus titer of PCV2 during the vaccine manufacturing process.

关键词： PCV2 Titration Hemagglutination Chicken red blood cells Bispecific nanobody

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