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Charge Transfer Platform and Catalytic Amplification of Phenanthroimidazole Derivative: A New Strategy for DNA Bases Recognition

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ANALYTICAL CHEMISTRY 2019年第18期91卷 11938-11945页

作者： Lu, Nannan Liu, He Huang, Rui Gu, Yue Yan, Xiaoyi zhang, Tinging Xu, Zhiqian Xu, Haixin Xing, Yue Song, Yu Li, xuwen zhang, Zhiquan Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China

Research about DNA composition has been concentrated on DNA damage in the past few decades. However, it still remains a great challenge to construct a rapid, facile, and accurate approach for simultaneously monitoring four DNA bases, guanine (G), adenine (A), thymine (T), and cytosine (C). Herein, a novel electrochemical sensor based on phenanthroimidazole derivative, 2-(4-bromophenyl)-1-phenyl-1H-phenanthro[9,10-d]-imidazole (PPI), is successfully fabricated by a simple electrochemical method. The bromophenyl group in PI could expand their aromatic plane, induce the pi-conjugated extension, and enhance the charge transfer and pi-pi interaction. The phenyl group at N1 position could regulate the intermolecular interaction, which could promote the possibility of intermolecular connection. The PPI polymer (poly(PPI)) with pi-electron enriched conjugation architecture has been applied in simultaneous determination of G, A, T, and C in neutral solution by square wave voltammetry (SWV) method with well-separated peak potentials at 0.714, 1.004, 1.177, and 1.353 V, respectively. The sensor functionalized with poly(PPI) exhibits wide linear response for G, A, T, and C in the concentration ranges of 3-300, 1-300, 30-800, and 20-750 mu M, respectively. With favorable selectivity, stability, and reproducibility, the sensor is successfully utilized to monitor four DNA bases in real samples, displaying a promising prospect for electrochemical sensing devices.

关键词： Cytosine Genetics Electrodes Oxidation Acid and base chemistry

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The immunopotentiator CVC1302 enhances immune efficacy and protective ability of foot-and-mouth disease virus vaccine in pigs

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VACCINE 2018年第52期36卷 7929-7935页

作者： Chen, Jin Yu, Xiaoming Zheng, Qisheng Hou, Liting Du, Luping zhang, Yuanpeng Qiao, xuwen Hou, Jibo Huang, Kehe Nanjing Agr Univ Coll Vet Med Nanjing 210095 Jiangsu Peoples R China Nanjing Agr Univ China Inst Nutr & Metab Disorders Domest Anim & F Nanjing 210095 Jiangsu Peoples R China Jiangsu Acad Agr Sci Inst Vet Immunol & Engn Nanjing 210014 Jiangsu Peoples R China Jiangsu Coinnovat Ctr Prevent & Control Important Yangzhou 225009 Jiangsu Peoples R China

The immunological enhancement characteristics of the immunopotentiator CVC1302 were evaluated for foot-and-mouth disease virus (FMDV) inactivated vaccine in pigs. Eight-week-old piglets were vaccinated with the foot-and-mouth disease (FMD) vaccine alone (FMD-vaccine group) or with the addition of CVC1302 (FMD-CVC1302 group), and the serum liquid phase blocking ELISA (LPB-ELISA) antibody titers, IgG1 and IgG2 levels, and the levels of four cytokines secreted by peripheral blood lymphocytes were measured at 28 days post vaccination (dpv). In the FMD-CVC1302 group, the LPB-ELISA antibody titers, IgG1, and IgG2 titers, and IL-2, IL-4, IL-6, and IFN-gamma levels at 28 dpv were significantly higher than those in the FMD-vaccine group. The FMD-CVC1302 group had long-lasting antibody titers (>7.8 log(2)), lasting for at least 6 months. In addition, piglets were vaccinated with or without addition of CVC1302 to the FMD vaccine at three different doses (1, 1/3, and 1/9 of the standard vaccine dose) and the serum LPBELISA antibody and serum neutralizing (SN) antibody titers were detected at 28 dpv. Then all pigs were challenged with virulent FMDV for PD50 value, and the levels of FMDV-specific RNA copies for the two full-dose groups at 3 and 10 days post challenge (dpc) were measured. The LPB-ELISA and SN antibody titers for the three doses in the FMD-CVC1302 groups were significantly higher than those in the FMD-vaccine groups at the same doses (p < 0.05). Post-virus challenge, the FMDV-specific RNA copy number in the FMD-CVC1302 group was lower than that in the FMD-vaccine group at 3 and 10 dpc. The PD50 value was 15.85 for the FMD-CVC1302 group, which was obviously higher than that for the FMD-vaccine group (10.96), and in the 1/9-dose of FMD-vaccine group only 3/5 pigs were protected. These results indicate that CVC1302 can enhance the immune efficacy and protective ability of the FMD vaccine in pigs. (C) 2018 Elsevier Ltd. All rights reserved.

关键词： Foot-and-mouth disease virus Inactivated vaccine Immunopotentiator CVC1302 Immune efficacy

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An Decomposition Algorithm for Distribution Network Reconfiguration Schedule Considering Demand Response 10

An Decomposition Algorithm for Distribution Network Reconfig...

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IEEE PES Asia-Pacific Power and Energy Engineering Conference (APPEEC)

作者： Li, Zihao Wu, Wenchuan zhang, Boming Tai, Xue Yang, Jinxi Han, xuwen zhang, Chen Qian, Yuhao Wang, Bo Tsinghua Univ Dept Elect Engineer Beijing 100084 Peoples R China Elect Power Co Ltd State Grid Jiangsu Yangzhou Yangzhou 225000 Jiangsu Peoples R China

ISBN: (纸本)9781538656860

Network reconfiguration and demand response can both reduce the loss and improve the security of the distribution networks (DNs). In this paper, we describe a day-ahead DN reconfiguration schedule model considering demand response, in which network reconfiguration is conducted by distribution system operator (DSO) while the demand response strategies are realized by customer aggregators. To preserve information privacy and reduce computational burden, a two-level decomposition & coordination algorithm is proposed to solve this model, which is based on a log-barrier cost function. In the upper level, the DSO optimizes the network reconfiguration schedule, calculates the barrier cost of every load bus and then sends it to the corresponding customer aggregator. In the lower level, every customer aggregator exploits available controllable demands like heat pump (HP), electric vehicle (EV) to minimize the nodal daily cost based on the barrier cost calculated in DSO. Numerical tests verify the promising convergence of this decomposition algorithm and show a reduction of the total cost in DN.

关键词： distribution network network reconfiguration demand response decomposition algorithm

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Isothermal Stage Kinetics of Direct Coal Liquefaction for Shenhua Shendong Bituminous Coal

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ENERGY & FUELS 2015年第11期29卷 7526-7531页

作者： Jiang, Hongbo Wang, Xiuhui Shan, Xiangen Li, Kejian zhang, xuwen Cao, Xueping Weng, Huixin E China Univ Sci & Technol Res Inst Petr Proc Shanghai 200237 Peoples R China Natl Engn Lab Direct Coal Liquefact Shanghai 201108 Peoples R China

To study the direct coal liquefaction in the isothermal stage of Shenhua Shendong bituminous coal, the direct coal liquefaction with an iron-based catalyst was carried out in a 0.01 ton/day continuous tubular facility in the temperature range of 445-465 degrees C, with hydrogenated anthracene and wash oil as the solvent. An eight-lump kinetic model of the isothermal stage was proposed, and the kinetic parameters were estimated. The result showed that, in the isothermal stage, the oil was mainly obtained from pre-asphaltene and asphaltene (PAA) rather than from coal directly. The model was valid for the isothermal stage of direct coal liquefaction.

关键词： TEMPERATURE RANGE Mentoring Coal liquefaction absorbent oil kinetic model stream stage Kinetic parameters bituminous coal

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GSDcreator: An Efficient and Comprehensive Simulator for Genarating NGS Data with Population Genetic Information

GSDcreator: An Efficient and Comprehensive Simulator for Gen...

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IEEE International Conference on Bioinformatics and Biomedicine

作者： Shenjie Wang Jiayin Wang Xiao Xiao Xuanping zhang xuwen Wang Xiaoyan Zhu Xin Lai School of Computer Science and Technology Xi'an Jiaotong University School of Public Policy and Administration Xi'an Jiaotong University

ISBN: (纸本)9781728118680

In recent decades, NGS data analysis has become a major research field in bioinformatics, which presents great advantages in many application scenarios. Many algorithms and software were designed for analyzing the NGS data, while simulation datasets are urgently needed for testing software and optimizing their parameter configurations. Thus, a series of NGS data simulators have been published. However, the existing simulators cannot satisfy the requirements from many specific scenarios. First, they do not support many newly discovered variations. Second, complex structural variations are difficult to generate. In addition, along with the increase of population data, it is urgent to increase population information simulation. In this paper, we propose GSDcreator, a comprehensive NGS simulator that overcome the three weaknesses mentioned above. It can produce all known types of variation, where the complex of variations are also supported. Furthermore, it can capture many important real data features including population polymorphism, insert size distribution, adjacent site depth distribution, overall depth distribution, quality score distribution, amplification bias, sequencing errors and so on. It's highlighted that 1000 Genomes Project Database is taken as a reference and integrates population genetic information to simulate population polymorphism. To test the performance, we did a lot of experiments and found that simulated data produced by GSDcreator are quit mimic to the real sequencing data.

关键词： population genomics next-generation sequencing data analysis data simulator population information Metagenomics simulator testing software population genetics Population statistics simulated data Bioinformatics

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快速褪黑色素方法在免疫组织化学染色中的应用

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中国组织化学与细胞化学杂志 2021年第2期30卷 182-184页

作者：赖续文王蔚仝桂慧朱铭谊赖晃文王卓才张伟南部战区总医院病理科广州510010 南部战区总医院基础医学实验室广州510010

目的探讨在恶性黑色素瘤组织中如何快速褪除黑色素,便于进行免疫组织化学检测和观察。方法选取20例恶性黑色素瘤组织石蜡切片,分别应用0.5%高锰酸钾水浴法、0.25%高锰酸钾溶液滴入法、15%过氧化氢浸泡法和传统过氧化氢脱色法进行褪黑色... 详细信息

目的探讨在恶性黑色素瘤组织中如何快速褪除黑色素,便于进行免疫组织化学检测和观察。方法选取20例恶性黑色素瘤组织石蜡切片,分别应用0.5%高锰酸钾水浴法、0.25%高锰酸钾溶液滴入法、15%过氧化氢浸泡法和传统过氧化氢脱色法进行褪黑色素处理,处理过的切片分别进行免疫组织化学和HE染色,比较染色结果。结果以0.25%高锰酸钾溶液滴入法处理后的效果较为理想:HE染色显示切片完整,色素颗粒完全去除,组织形态和细胞形态清晰;免疫组织化学染色结果清晰易判读,具有较强的敏感性和特异性。结论0.25%高锰酸钾溶液滴入法可作为恶性黑色素瘤去除色素颗粒的有效方法。

关键词：免疫组织化学黑色素高锰酸钾草酸过氧化氢

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Characterisation of Ti(C, N)-based cermets with various nitrogen contents studied by EBSD/SEM and TEM

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JOURNAL OF ALLOYS AND COMPOUNDS 2017年 695卷 2857-2864页

作者： Shi, Zengmin Yin, Dezheng zhang, Dayong Liu, xuwen China Three Gorges Univ Coll Mat & Chem Engn Yichang 443002 Peoples R China Tianjin Recommend Sci & Technol Dev Co Ltd Tianjin 300050 Peoples R China

Ti(C, N)-based cermets with different N/(C + N) ratios were prepared by vacuum sintering. The effect of nitrogen content on microstructure of Ti(C, N) based cermets was analyzed using a combination of the electron backscatter diffraction in a scanning electron microscope (EBSD/SEM) and transmission electron microscope (TEM). The combination of XRD and SEM/BSE effectively showed the influence of the carbon and nitrogen contents on the core/rim microstructure features. EBSD maps gave a vivid image for hard phase grains, especially the carbonitride grain size distribution, orientation distribution and crack propagation. The combination of EBSD/SEM and TEM revealed a highly coherent interface between the core and rim. The carbonitride particle and the binder had a large high angle boundary and a coherent interface. A constant interface orientation of the carbonitride particle and binder was characterized. The major factors determining the transverse rupture strength and the fracture toughness were discussed in terms of the crack propagation. (C) 2016 Elsevier B. V. All rights reserved.

关键词： Ti(C, N)-based cermet EBSD Orientation Nitrogen content Structure

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掺兑蒽油加氢制备煤直接液化循环溶剂

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石油学报（石油加工） 2016年第2期32卷 369-374页

作者：白雪梅李克健章序文高山松冯玉艳中国神华煤制油化工有限公司上海研究院煤炭直接液化国家工程实验室上海201108

在煤直接液化循环溶剂加氢原料中掺兑煤焦油蒽油,采用300mL固定床加氢实验装置考察蒽油掺兑量对循环溶剂性质的影响;采用0.5L高压釜煤液化实验考察蒽油掺兑量对煤液化反应的影响。结果表明,在相同的加氢条件下,在煤直接液化循环溶剂加... 详细信息

在煤直接液化循环溶剂加氢原料中掺兑煤焦油蒽油,采用300mL固定床加氢实验装置考察蒽油掺兑量对循环溶剂性质的影响;采用0.5L高压釜煤液化实验考察蒽油掺兑量对煤液化反应的影响。结果表明,在相同的加氢条件下,在煤直接液化循环溶剂加氢原料中掺兑5%(质量分数)的蒽油,循环溶剂的芳碳率(fa)降幅3.37%,供氢指数(PDQI)增幅3.68%,供氢性能得到提高,但加氢反应氢耗增加,循环溶剂密度、黏度及硫、氮含量增大。采用此循环溶剂进行煤液化时,煤的转化率提高了0.15%,煤液化油收率增加了0.98%。随着蒽油掺兑质量分数的增加,循环溶剂供氢性能逐渐减弱,煤液化转化率和液化油收率逐渐减小,循环溶剂密度、黏度及硫、氮含量持续增大。

关键词：煤直接液化蒽油循环溶剂溶剂加氢供氢性能

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金属有机多面体基包覆型微球制备及CO2环加成反应

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南京工业大学学报（自然科学版） 2020年第4期42卷 536-542页

作者：吕保帅周洁蒙旭雯张竹修汤吉海崔咪芬乔旭南京工业大学化工学院材料化学工程国家重点实验室江苏南京211800

采用原位合成法在铜基海藻酸-壳聚糖-SiO2(Cu-ALG-CS-SiO2)内部生长金属有机多面体(MOP),制备MOP基包覆型微球MOP-ALG-CS-SiO2。通过X线衍射(XRD)、N2吸附-脱附、透射电子显微镜(TEM)等方法对MOP-ALG-CS-SiO2的晶形、孔径和形貌进行分... 详细信息

采用原位合成法在铜基海藻酸-壳聚糖-SiO2(Cu-ALG-CS-SiO2)内部生长金属有机多面体(MOP),制备MOP基包覆型微球MOP-ALG-CS-SiO2。通过X线衍射(XRD)、N2吸附-脱附、透射电子显微镜(TEM)等方法对MOP-ALG-CS-SiO2的晶形、孔径和形貌进行分析并考察MOP-ALG-CS-SiO2催化环氧化合物与CO2环加成制备环状碳酸酯的反应性能。结果表明:MOP-ALG-CS-SiO2具有无定形的介孔结构,MOP在MOP-ALG-CS-SiO2中高度分散,解决了MOP基复合材料中普遍存在的稳定性和分散性不好的问题。MOP-ALG-CS-SiO2在催化环氧丙烷与CO2环加成反应中,环氧丙烷转化率为84.1%、碳酸丙烯酯选择性为99.7%,催化性能优于MOP、MOP-ALG-CS和HKUST-1。此外,MOP-ALG-CS-SiO2在催化其他类型环氧化物与CO2环加成反应中均表现出较高的催化活性和产物选择性。

关键词：金属有机多面体(MOP) 原位合成介孔微球环加成反应

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Mechanisms of acetylcholine receptor gene eat-2 in regulating the development of ***

Mechanisms of acetylcholine receptor gene eat-2 in regulatin...

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第五届中国秀丽线虫大会

作者： xuwen Cao Yusu Xie Pengchi zhang Liusuo zhang CAS Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciences Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Center for Ocean Mega-Science Chinese Academy of Sciences University of Chinese Academy of Sciences

＜正＞How the amount and nutritional composition of diets influence organismal development,fecundity and health are largely *** axenic chemically defined food *** maintenance medium（CeMM） is a powerful experiment material to study the impact of diet and nutrition on life-history traits such as growth and *** studies have shown that the eat-2 gene,

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