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当代体育科技 2023年第1期13卷 104-107页

作者：李绪稳杨海航张治远西藏民族大学陕西咸阳712082

大学生作为新时代国家优质人才,推进体育强国建设的后备力量,基于健康中国视域下,大学生体质健康水平整体下滑已成为不争事实,研究大学生体质健康现状和提升对策,是高校体育教学改革的新方向。因此,该文选用契约学习法,明确其基本内涵... 详细信息

大学生作为新时代国家优质人才,推进体育强国建设的后备力量,基于健康中国视域下,大学生体质健康水平整体下滑已成为不争事实,研究大学生体质健康现状和提升对策,是高校体育教学改革的新方向。因此,该文选用契约学习法,明确其基本内涵与特征,充分认知契约学习法在高校大学生体质健康中的应用现状与价值,并从诊断大学生体质健康情况,师生共同诊断学习需求,结合学习需要制定学习目标,确定学习资源与学习策略,确定具体教学计划,商讨并签订学习契约,学习契约执行与评价7个维度,阐明契约学习法的应用流程。最后,提出契约学习法在高校大学生体质健康中应用的注意事项,包括学习契约需共同商讨确立,学习契约需不断改进完善,学习契约兼具优势和局限等,以改善大学体育教学效果,提升当代大学生体质健康水平。

关键词：契约学习法高校大学生体质健康体育教学

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Observation of Dirac nodal line states in topological semimetal candidate PrSbTe

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Physical Review B 2024年第4期109卷 045113-045113页

作者： Dengpeng Yuan Dajian Huang Xin Ma Xu Chen Huifen Ren Yun zhang Wei Feng Xiegang Zhu Bo Wang xuwen He Jian Wu Shiyong Tan Qunqing Hao Qiang zhang Yi Liu Qin Liu Zhengtai Liu Chao Cao Qiuyun Chen Xinchun Lai Science and Technology on Surface Physics and Chemistry Laboratory Mianyang 621907 China Center for Correlated Matter and School of Physics Zhejiang University Hangzhou 310058 China Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100190 China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research Institute Chinese Academy of Sciences Shanghai 201210 China

The interplay among topology, crystal symmetry, magnetic order, and strong electron correlation can give rise to a plethora of exotic physical phenomena. The ZrSiS family is known as typical topological Dirac semimetals, among them LnSbTe (Ln denotes lanthanide) compounds exhibit intriguing characteristics due to the presence of Ln 4f electrons, resulting in quantum states and unique properties. In this paper, the topological electronic structure of PrSbTe is systematically studied by angle-resolved photoemission spectroscopy (ARPES), combined with magnetic, specific heat measurements, and band structure calculations. The detailed three-dimensional electronic structure of PrSbTe has been obtained, and a diamond-shaped Fermi surface and multiple Dirac nodal lines have been observed, which are in remarkable agreement with theoretical calculations. Moreover, the 4f electrons in PrSbTe are rather localized, which can be revealed by on-resonant ARPES data and further confirmed by the rather small Sommerfeld coefficient of γ=2.6231mJ/molK2. Our results provide more detailed information about the LnSbTe family, which gives a deeper understanding of the interaction between Ln 4f electrons and the topological states.

关键词： Electronic structure Fermi surface Topological materials Dirac semimetal Node-line semimetals Angle-resolved photoemission spectroscopy Magnetic techniques Specific heat measurements

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Manginoids A-G: Seven Monoterpene-Shikimate-Conjugated Meroterpenoids with a Spiro Ring System from Guignardia mangiferae

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ORGANIC LETTERS 2017年第21期19卷 5956-5959页

作者： Chen, Keliang zhang, xuwen Sun, Weiguang Liu, Junjun Yang, Jing Chen, Chunmei Liu, Xiulan Gao, Limin Wang, Jianping Li, Hua Luo, Zengwei Xue, Yongbo Zhu, Hucheng zhang, Yonghui Huazhong Univ Sci & Technol Tongji Med Coll Sch Pharm Hubei Key Lab Nat Med Chem & Resource Evaluat Wuhan 430030 Hubei Peoples R China Huazhong Univ Sci & Technol Tongji Med Coll Tongji Hosp Wuhan 430030 Hubei Peoples R China Humanwell Healthcare Grp Co Ltd Wuhan 430073 Hubei Peoples R China Chinese Acad Sci Kunming Inst Bot State Key Lab Phytochem & Plant Resources West Ch Kunming 650204 Yunnan Peoples R China

Manginoids A-G (1-7), seven monoterpene-shikimate-conjugated meroterpenoids with a spiro ring system, were isolated from Guignardia mangiferae. Compounds 1-4 are four isomers with epimeric and double-bond isomeric features possessing a 6-oxaspiro[bicyclo[3.2.1]octane-3,5 '-indene] ring, which represent the first examples of spiro meroterpenoids bearing a bridged spirocyclohexanedione moiety. Compounds 5 and 6 possess an unexpected 2,4-dioxatricyclo[3.3.1.0(3,7)]nonane motif, which fuses with a 6-oxabicyclo[3.2.1]octane moiety. Compound 1 exhibits inhibitory activities against 11 beta-hydroxysteroid dehydrogenase type 1 with an IC50 value of 0.84 mu M.

关键词： Carbon Circular dichroism spectroscopy Chemical structure Absolute configuration Inhibition

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结构性鼻塞的病因学及机制研究进展

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临床耳鼻咽喉头颈外科杂志 2021年第7期35卷 666-672页

作者：张郅瑾杨旭雯李绚刘锦峰首都医科大学附属北京朝阳医院(第三临床医学院) 北京100020 首都医科大学附属北京朝阳医院耳鼻咽喉头颈外科

鼻塞(nasal obstruction)是耳鼻喉科的常见主诉,是多数鼻科疾病的共有症状,可严重影响患者的睡眠及生活质量[1]。鼻塞可以由炎症(过敏性及感染性)、占位病变、鼻气道结构异常、药物性、生理性、感觉神经性及心理性等因素引起[2-3]。其... 详细信息

鼻塞(nasal obstruction)是耳鼻喉科的常见主诉,是多数鼻科疾病的共有症状,可严重影响患者的睡眠及生活质量[1]。鼻塞可以由炎症(过敏性及感染性)、占位病变、鼻气道结构异常、药物性、生理性、感觉神经性及心理性等因素引起[2-3]。其中黏膜炎症引起的鼻塞多数持续时间不会太长,为急性或亚急性,药物治疗可有效缓解[4]。但对于鼻塞持续时间超过半年以上的慢性鼻塞患者,其病因更为复杂且顽固。

关键词：鼻塞鼻气道病因学

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A new nanobody-enzyme fusion protein-linked immunoassay for detecting antibodies against influenza A virus in different species

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JOURNAL OF BIOLOGICAL CHEMISTRY 2022年第12期298卷 102709页

作者： Ji, Pinpin Wang, Kun zhang, Lu Yan, Zhenda Kong, Min Sun, xuwen zhang, Qiang Zhou, Ning Liu, Baoyuan Zhou, En-Min Sun, Yani Wang, Xinjie Zhao, Qin Northwest A&F Univ Dept Vet Prevent Med Coll Vet Med Yangling Shaanxi Peoples R China Chinese Acad Agr Sci Guangdong Lab Lingnan Modern Agr Agr Genom Inst Shenzhen Shenzhen BranchGenome Anal LabMinist Agr & Rura Shenzhen Peoples R China

Circulation of influenza A virus (IAV), especially within poultry and pigs, continues to threaten public health. A simple and universal detecting method is important for monitoring IAV infection in different species. Recently, nanobodies, which show advantages of easy gene editing and low cost of production, are a promising novel diagnostic tool for the monitoring and control of global IAVs. In the present study, five nanobodies against the nucleoprotein of H9N2 IAV were screened from the immunized Bactrian camel by phage display and modified with horseradish peroxidase (HRP) tags. Out of which, we determined that H9N2-NP-Nb5-HRP can crossreact with different subtypes of IAVs, and this reaction is also blocked by positive sera for antibodies against different IAV subtypes. Epitope mapping showed that the nanobody-HRP fusion recognized a conserved conformational epitope in all subtypes of IAVs. Subsequently, we developed a nanobody-based competitive ELISA (cELISA) for detecting anti-IAV antibodies in different species. The optimized amount of coating antigen and dilutions of the fusion and testing sera were 100 ng/well, 1:4000, and 1:10, respectively. The time for operating the cELISA was approximately 35 min. The cELISA showed high sensitivity, specificity, reproducibility, and stability. In addition, we found that the cELISA and hemagglutination inhibition test showed a consistency of 100% and 87.91% for clinical and challenged chicken sera, respectively. Furthermore, the agreement rates were 90.4% and 85.7% between the cELISA and commercial IEDXX ELISA kit. Collectively, our developed nanobody-HRP fusion–based cELISA is an ideal method for monitoring IAV infection in different species.

关键词： influenza A virus antibodies against IAVs nucleoprotein nanobody nanobody-HRP fusion protein competitive ELISA

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WRKY15 Suppresses Tracheary Element Differentiation Upstream of VND7 During Xylem Formation

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PLANT CELL 2020年第7期32卷 2307-2324页

作者： Ge, Shating Han, Xiaofei Xu, xuwen Shao, Yiming Zhu, Qiankun Liu, Yidong Du, Juan Xu, Juan zhang, Shuqun Zhejiang Univ Coll Life Sci State Key Lab Plant Physiol & Biochem Hangzhou 310058 Zhejiang Peoples R China Nanjing Agr Univ Coll Plant Protect Nanjing 210095 Jiangsu Peoples R China Univ Missouri Div Biochem Columbia MO 65211 USA

Arabidopsis WRKY15 functions upstream of VND7, an important regulator of tracheary element (TE) differentiation, to suppress TE formation during plant vascular development. Formation of the vascular cylinder, a structure critical to water and nutrient transport in higher plants, is highly regulated. Here we identify WRKY15 as an important regulator that suppresses tracheary element (TE) differentiation in Arabidopsis (Arabidopsis thaliana). Overexpression of WRKY15 resulted in discontinuous protoxylem vessel files and TEs with reduced spiral wall thickening/lignification. Expression of a dominant-negative WRKY15 variant, WRKY15-EAR, led to extra protoxylem vessels and ectopic TEs with increased spiral wall thickening/lignification. Ectopic TE formation in the root cortex and hypocotyl/leaf epidermis reveals that the suppression of WRKY15 is sufficient to trigger the transdifferentiation of other types of cells to TEs. Expression profiling, RT-qPCR, and reporter analyses revealed that WRKY15 suppresses the expression of VASCULAR-RELATED NAC DOMAIN7 (VND7), a master transcriptional regulator that promotes TE differentiation. We propose that WRKY15 negatively regulates VND7 expression indirectly based on (1) the absence of a W-box in the promoter of VND7 and (2) the observation that WRKY15 and VND7 are expressed in different cells in the vascular cylinder, with WRKY15 expressed in the procambial cells and VND7 in the protoxylem poles of procambium and differentiating TEs. Future research is needed to reveal the details underlying the interaction of WRKY15 and VND7 in plant vascular development.

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托法替布联合来氟米特治疗类风湿关节炎疗效及对血清Janus激酶/信号转导和转录激活因子信号通路相关蛋白和基质金属蛋白酶水平的影响

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中华风湿病学杂志 2022年第11期26卷 737-744页

作者：汪珊查旭雯阮圣霆姚寿林张小雨安徽医科大学第三附属医院(合肥市第一人民医院)风湿免疫科合肥230001

目的探讨托法替布联合来氟米特治疗RA的临床疗效及其对患者血清Janus激酶(JAK)/信号转导和转录激活因子(STAT)信号通路相关蛋白和MMPs水平的影响。方法本研究为前瞻性病例对照研究。筛选2020年3月至2021年9月我院收治的80例RA患者纳入... 详细信息

目的探讨托法替布联合来氟米特治疗RA的临床疗效及其对患者血清Janus激酶(JAK)/信号转导和转录激活因子(STAT)信号通路相关蛋白和MMPs水平的影响。方法本研究为前瞻性病例对照研究。筛选2020年3月至2021年9月我院收治的80例RA患者纳入研究。按随机数字表法分成观察组40例与对照组40例。观察组采取托法替布联合来氟米特治疗,对照组单用来氟米特治疗。连续治疗12周后观察2组疗效。比较治疗前后2组典型临床表现[关节疼痛视觉模拟量表(VAS)评分、压痛关节数、肿胀关节数、晨僵时间]、DAS28评分、36项健康调查简表(SF-36)总分及血清JAK3、STAT3、IL-6、IL-17、MMPs水平;并统计2组药物不良反应情况。统计学处理采用χ^(2)检验、配对样本t检验、独立样本t检验、Fisher确切概率法。结果治疗4、8、12周后,观察组ACR20达标率分别为37.5%(15/40),62.5%(25/40),80.0%(32/40),均高于同期对照组[17.5%(7/40)、37.5%(15/40)、57.5%(23/40);χ^(2)=4.01,P=0.045,χ^(2)=5.00,P=0.025,χ^(2)=4.71,P=0.030]。治疗8、12周后,观察组ACR50达标率分别为35.0%(14/40)、47.5%(19/40),均高于同期对照组[15.0%(6/40),20.0%(8/40);χ^(2)=4.27,P=0.039,χ^(2)=6.76,P=0.009]。治疗后,观察组关节疼痛VAS评分、压痛关节数、肿胀关节数、DAS28评分、SF-36总分均低于对照组(t=5.55,P<0.001;t=9.98,P<0.001;t=11.77,P<0.001;t=4.50,P<0.001;t=5.28,P<0.001),晨僵时间均短于对照组(t=4.76,P<0.001)。治疗后,观察组血清JAK3、STAT3、IL-6、IL-17、MMP-3、MMP-9及MMP-13水平分别为(2354±476)pg/ml,(1.04±0.17)ng/ml,(12.4±2.8)pg/ml,(30±5)pg/ml,(65±14)μg/L,(76±12)μg/L,(11.5±1.8)μg/L,均低于对照组[(2715±584)pg/ml,(1.22±0.29)ng/ml,(16.8±3.6)pg/ml,(40±7)pg/ml,(98±15)μg/L,(123±20)μg/L,(14.9±2.8)μg/L,t=3.03,P<0.05;t=3.39,P<0.05;t=6.10,P<0.05;t=7.35,P<0.05;t=10.17,P<0.05;t=12.74,P<0.05;t=6.46,P<0.05]。观察组不良反应率[35.0%(14/40)]较对照组[27.5%(11/40)]差异无统计学意义(χ^(2)=0.52,P=0.469)。结论托法替布联合来氟米特治疗RA的总体疗效满意,是改善患者临床表现、疾病活动度及生活质量的安全有效途径,其作用可能与其显著下调血清JAK/STAT信号通路相关蛋白和MMPs的表达水平有关。

关键词：关节炎,类风湿 Janus激酶3 STAT3转录因子基质金属蛋白酶托法替布来氟米特

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HAZOP分析在火炸药工艺风险控制中的应用

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固体火箭技术 2024年第6期47卷 774-783页

作者：史喆邢峰康红艳赵媛媛马志伟王旭文张健杨玉林中国五洲工程设计集团有限公司北京100053 哈尔滨工业大学化工与化学学院新能源转换与存储关键材料技术工业和信息化部重点实验室哈尔滨150001

火炸药产品的加工制造过程具有显著的高能量性、高危险性和高敏感性,其工艺安全性和可靠性要求极为严格,需要对其设计、制造和生产全生命周期进行全方位分析,并做出相应的应急措施。危险与可操作性分析(HAZOP)是一种广泛应用于危险化学... 详细信息

火炸药产品的加工制造过程具有显著的高能量性、高危险性和高敏感性,其工艺安全性和可靠性要求极为严格,需要对其设计、制造和生产全生命周期进行全方位分析,并做出相应的应急措施。危险与可操作性分析(HAZOP)是一种广泛应用于危险化学品领域的安全分析方法,能够对工业过程或单元操作进行审查,识别、评估和预防潜在的危险或操作性问题,从而根据不同的问题制定相应的安全和管理措施,最终达到降低事故发生概率的目的。详述了HAZOP分析的流程和优势,就HAZOP分析应用于炸药、发射药、固体推进剂等涉火产品工艺安全风险控制领域的情况进行论述,对其在火炸药工艺风险控制中的适用性和目前在应用过程中存在的问题进行论证和展望,未来应加快火炸药产线物料的安全特性研究,提高HAZOP分析的全面性,加强HAZOP分析与故障模式与影响分析(FMEA)、保护层分析(LOPA)、安全完整性等级(SIL)及定量风险评估(QRA)等方法协同应用,使火炸药工艺安全风险控制得到进一步提升。

关键词： HAZOP分析火炸药工艺安全风险控制

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A comprehensive analysis of the microbial diversity in natural and engineered ecosystems based on high-throughput sequencing of 16S rRNA gene

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INTERNATIONAL BIODETERIORATION & BIODEGRADATION 2019年 140卷 160-168页

作者： Xia, Yu He, xuwen Feng, Zeshen zhang, Qing Yang, Hongying China Univ Min & Technol Beijing Sch Chem & Environm Engn Beijing 100083 Peoples R China Northeastern Univ Sch Met Shenyang 110819 Liaoning Peoples R China

Understanding microbial diversity across different ecosystems is important for discerning the mechanisms shaping the microbial community assembly. However, knowledge on differentiations of microbial diversity in various habitats especially engineered systems remains very limited. A comprehensive comparison of microbial diversity across natural and engineered environments, namely heavy metal polluted farm soil, forest, wastewater treatment plants (WWTPs), anaerobic digesters (ADs) and the Caspian Sea sediments, was made base on a collection of 115 samples from seven published 16S rRNA gene datasets generated by high-throughput sequencing. In each sample, 16,590 and 19,771 qualified sequences were detected, and they were classified into 615 to 3,162 operational taxonomic units (OTUs). The microbial communities in the engineered systems especially the AD showed relatively low alpha taxonomic and phylogenetic diversity. A total of 49 genera were present in more than 80% of the samples, accounting for 6.0% in population number and 25.6% in relative abundance (RA). The average abundance-weighted rRNA operon copy number of the communities from ADs and a food wastewater treatment plant (FW) was higher than that of others, suggesting that high loading rate of pollutants in ADs and FW resulted in relatively high abundance of r-strategists. Among 104 typical human bacterial pathogens (HBPs), 2 to 25 HBPs were detected in each sample, accounting for 0.05%-21.34% in RA. Mycobacterium phlei was widely present in 111 samples with an average RA of 0.12%. The number and RA of all the HBPs in each sample did not show an obvious ecosystem type related pattern. Results of detrended correspondence analysis (DCA) and cluster analysis showed that the microbial communities clustered based on habitat types. The communities of WWTPs and soil were very similar, falling into one cluster. For any two communities from the datasets, 75.6% of their beta nearest taxon index (beta NTI) values were h

关键词： Microbial community Natural environments Engineered systems Diversity Environmental conditions High-throughput sequencing

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伪狂犬活/灭活病毒接种小鼠诱导免疫效力及差异因子比较

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南京农业大学学报 2023年第1期46卷 106-111页

作者：章晨昕于晓明侯立婷张元鹏乔绪稳侯继波黄克和郑其升陈瑾南京农业大学动物医学院江苏南京210095 江苏省农业科学院动物免疫工程研究所/国家兽用生物制品工程技术研究中心江苏南京210014 江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地江苏南京210014

[目的]本研究旨在比较活/灭活病毒进入体内后对免疫系统活化的差异,为免疫增强剂的筛选和评价建立高效动物模型。[方法]本试验利用伪狂犬病毒(PRV)Bartha-K61株建立小鼠伪狂犬人工感染模型,筛选活/灭活病毒接种小鼠后免疫相关差异因子。... 详细信息

[目的]本研究旨在比较活/灭活病毒进入体内后对免疫系统活化的差异,为免疫增强剂的筛选和评价建立高效动物模型。[方法]本试验利用伪狂犬病毒(PRV)Bartha-K61株建立小鼠伪狂犬人工感染模型,筛选活/灭活病毒接种小鼠后免疫相关差异因子。将PRV Bartha-K61活毒以不同剂量、不同方式感染小鼠,通过计算小鼠存活率确定最佳感染剂量与感染方式;将筛选的最佳剂量的伪狂犬病毒活毒与相同剂量下灭活病毒按照前述筛选的最佳感染方式接种小鼠,利用ELISA试剂盒检测免疫7 d后小鼠血清中TNF-α、IL-1β、IFN-β和IL-6水平;利用PRV gB ELISA检测免疫7 d后小鼠血清PRV特异性gB抗体水平;利用灭活PRV作为特异性抗原进行淋巴细胞增殖试验并计算各组淋巴细胞刺激指数(SI)。[结果]在保证小鼠存活的前提下,6~8周龄小鼠的PRV的安全感染剂量为5×104TCID50;同等剂量下,相比于肌肉和腹腔注射,皮下注射为最安全的感染方式;同等剂量的活毒和灭活病毒接种小鼠后,与灭活病毒组相比,活病毒感染7 d后小鼠血清中TNF-α、IL-1β和IFN-β水平显著升高(P<0.001,P<0.01),与灭活病毒组相比,活病毒组小鼠血清PRV gB特异性抗体IgG水平和SI也显著升高(P<0.05,P<0.001);不同灭活方式间各细胞因子和SI差异不显著(P>0.05)。[结论]成功建立小鼠伪狂犬人工感染模型,明确活/灭活伪狂犬病毒接种小鼠后,机体早期存在与免疫相关差异因子,为后续免疫增强剂的评价筛选提供依据。

关键词：伪狂犬病毒小鼠模型免疫效力差异因子

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