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Corrigendum to “Occurrence, transfer rule, and dietary risk assessment of aflatoxins in medicinal and edible seeds” [Food Bioscience 63 (2025) 105830]

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Food Bioscience 2025年 68卷

作者： Jianxin Lv Huiru zhang xuwen Wang Jiaoyang Luo Yudan Wang Dandan Kong Chuang Wang Mengyue Guo Meihua Yang Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of Education Institute of Medicinal Plant Development Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100193 China Hainan Branch of the Institute of Medicinal Plant Development Chinese Academy of Medical Sciences & Peking Union Medical College Haikou 570311 China

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“血-脉-心-神”一体观辨治糖尿病心肌病思路探析

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中华中医药杂志 2024年第2期39卷 831-835页

作者：黄旭文严志鹏施琦耿小飞漫富婧祁含章范新彪朱亚萍张军平天津中医药大学第一附属医院天津300381 国家中医针灸临床医学研究中心天津300381

糖尿病心肌病(DCM)早期发病隐匿,易被忽视,延误治疗,后期心肌严重损伤,预后较差。除对症治疗外,目前尚无统一有效的特异性治疗方案。基于“血-脉-心-神”一体观,DCM病程可分为血脉共病,心体受损,体用俱衰、神机失用3个阶段。治疗时,抓... 详细信息

糖尿病心肌病(DCM)早期发病隐匿,易被忽视,延误治疗,后期心肌严重损伤,预后较差。除对症治疗外,目前尚无统一有效的特异性治疗方案。基于“血-脉-心-神”一体观,DCM病程可分为血脉共病,心体受损,体用俱衰、神机失用3个阶段。治疗时,抓住各阶段主要矛盾,辨证施治。在血脉共病阶段,运用活血解毒法,调血畅脉,及早截断病程;心体受损阶段,软坚散结,调气和血以保护心肌、延缓病程;心之体用俱衰,神机失用阶段,育心保脉,调神安神以缓解症状、改善预后。以期为中医药分阶段、全时程防治DCM提供新的思路。

关键词：糖尿病心肌病 “血-脉-心-神”一体观分阶段辨治思路中医药

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Magnetic dispersive solid-phase extraction for the determination of three different glycosides in the Kang'ai injection

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MICROCHEMICAL JOURNAL 2019年 146卷 575-581页

作者： Li, Peng Ye, Junfeng zhang, Yue Wang, Ziqi Ren, Sujuan Jin, Yongri Li, xuwen Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China Jilin Univ Hosp 1 Changchun 130012 Jilin Peoples R China

In this work, the content of flavonoid glycosides and astragalosides in a Chinese medicine injection was determined by using the magnetic dispersive solid phase extraction method coupled with high performance liquid chromatography with an evaporative light-scattering detector (HPLC/ELSD). Multi-walled carbon nanotubes (MWCNTs) modified by Fe3O4 nanoparticles obtained in a one-step synthetic procedure, were used as a sorbent to conveniently remove the analytes from water. Single factor experiments were performed to investigate the factors that affected the extraction efficiency, such as the hydroxyl content of the modified MWCNTs, the amount of sorbent, the type of elution solvent, the volume of elution solvent, sample pH and salting-out effect. Under the appropriate optimal conditions, the limits of detection (LODs) and limits of quantification (LOQs) for the three glycosides ranged from 0.16 mu g/mL to 0.60 mu g/mL and 0.58 mu g/mL to 1.24 mu g/mL, respectively. Moreover, the intra- and interday precision ranged from 1.9% to 2.9% and 2.4% to 4.5%, respectively. Meanwhile, the recoveries of spiked samples were measured, which ranged from 91.1% to 96.2% with relative standard deviations (RSDs) ranging from 2.8% to 3.8%. Accordingly, the application of the proposed method for the analysis of Chinese medicine injection samples offered several advantages: an acceptable precision, low LODs and good recoveries. This work might provide a research basis for the quality control of Chinese medicine injections and the further study of the Kang'ai injection.

关键词： Dispersive solid phase extraction Evaporative light-scattering detector Kang'ai injection

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Novel androgen receptor antagonist identified by structure-based virtual screening, structural optimization, and biological evaluation

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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY 2020年 192卷 112156-000页

作者： Tang, Qin Fu, Weitao zhang, Minkui Wang, Ercheng Shan, Lvhu Chai, Xin Pang, Jinping Wang, xuwen Xu, Xiaohong Xu, Lei Li, Dan Sheng, Rong Hou, Tingjun Zhejiang Univ Coll Pharmaceut Sci Hangzhou 310058 Zhejiang Peoples R China Chinese Acad Sci Univ Chinese Acad Sci Zhejiang Canc Hosp Inst Canc Res & Basic Med SciCanc Hosp Hangzhou 310022 Zhejiang Peoples R China Jiangsu Univ Technol Sch Elect & Informat Engn Inst Bioinformat & Med Engn Changzhou 213001 Jiangsu Peoples R China

Androgen receptor (AR) plays important roles in the development of prostate cancer (PCa), and therefore it has been regarded as the most important therapeutic target for both hormone-sensitive prostate cancer (HSPC) and advanced PCa. In this study, a novel hit (C18) with IC50 of 2.4 mu M against AR transcriptional activity in LNCaP cell was identified through structure-based virtual screening based on molecular docking and free energy calculations. The structure-activity relationship analysis and structural optimization of C18 resulted in the discovery of a structural analogue (AT2), a more potent AR antagonist with 16-fold improved anti-AR potency. Further assays indicated that AT2 was capable of effectively inhibiting the transcriptional function of AR and blocking the nuclear translocation of AR like the second-generation AR antagonists. The antagonists discovered in this study may be served as the promising lead compounds for the development of AR-driven PCa therapeutics. (c) 2020 Elsevier Masson SAS. All rights reserved.

关键词： Prostate cancer Androgen receptor Structure-based virtual screening Antagonist Molecular docking

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靶向B细胞的口蹄疫GHloop多肽抗原的制备及免疫效力鉴定

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中国动物传染病学报 2023年第3期31卷 74-81页

作者：侯立婷张媛媛于晓明杜露平张元鹏程海卫乔绪稳李丁郑其升陈瑾江苏省农业科学院动物免疫工程研究所南京210014 江苏省农业科学院国家兽用生物制品工程技术研究中心南京210014 兽用生物制品(泰州)国泰技术创新中心泰州225300 省部共建国家重点实验室培育基地—江苏省食品质量安全重点实验室南京210014

本研究将葡萄球菌A蛋白人工合成类似物的二聚体(DD)与口蹄疫的GHloop串联,按照大肠杆菌偏好性密码子优化合成GHloop-DD,并评价其免疫原性。将GHloop-DD插入到原核表达载体pET-32a中,鉴定阳性后转入*** BL21进行诱导表达。利用SDS-PAGE与... 详细信息

本研究将葡萄球菌A蛋白人工合成类似物的二聚体(DD)与口蹄疫的GHloop串联,按照大肠杆菌偏好性密码子优化合成GHloop-DD,并评价其免疫原性。将GHloop-DD插入到原核表达载体pET-32a中,鉴定阳性后转入*** BL21进行诱导表达。利用SDS-PAGE与Western blot对目的基因表达产物的可溶性进行分析。利用镍亲和层析柱纯化目的蛋白;利用BCA试剂盒测定重组蛋白的浓度,将重组蛋白(2.5μg/只)与206佐剂乳化,制备疫苗,免疫5周龄小鼠,同时设商品化合成肽苗对照与空白对照。免疫后28 d采集试验小鼠血清,利用口蹄疫病毒多肽ELISA抗体检测试剂盒检测免疫小鼠血清的抗体水平;利用流式细胞仪测定淋巴结B细胞的数量及骨髓中长寿浆细胞的数量;利用荧光定量检测相关趋化因子的表达水平;利用细胞因子检测试剂盒检测脾脏淋巴细胞刺激后上清液中的细胞因子的水平。SDS-PAGE结果表明,GHloop-DD基因在大肠杆菌中以可溶形式获得高水平表达;Western blot结果显示,表达的重组蛋白能够与特异性抗体发生反应。免疫试验结果表明,GHloop-DD不但能够刺激免疫小鼠产生高水平的多肽特异性ELISA抗体,而且能够提高淋巴结B细胞的数目及相关趋化因子水平,使骨髓中长寿浆细胞的数量和脾脏淋巴细胞刺激后上清液中细胞因子的水平显著提高。以上结果表明,本研究制备的GHloop-DD具有良好的免疫原性,为口蹄疫多肽疫苗的研制提供了新的方向。

关键词： GHloop-DD 体液免疫 B细胞长寿浆细胞

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Identification of mechanisms conferring an enhanced immune response in mice induced by CVC1302-adjuvanted killed serotype O foot-and-mouth virus vaccine

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VACCINE 2019年第43期37卷 6362-6370页

作者： Du, Luping Yu, Xiaoming Hou, Liting zhang, Dong zhang, Yuanpeng Qiao, xuwen Hou, Jibo Chen, Jin Zheng, Qisheng Jiangsu Acad Agr Sci Inst Vet Immunol & Engn Nanjing 210014 Jiangsu Peoples R China Jiangsu Acad Agr Sci Natl Res Ctr Engn & Technol Vet Biol Nanjing 210014 Jiangsu Peoples R China Jiangsu Coinnovat Ctr Prevent & Control Important Yangzhou 225009 Jiangsu Peoples R China Minist Sci & Technol Nanjing Jiangsu Key Lab Food Qual Nanjing 210014 Jiangsu Peoples R China Minist Sci & Technol Nanjing Safety State Key Lab Cultivat Base Nanjing 210014 Jiangsu Peoples R China Shandong Prov Ctr Anim Dis Control & Prevent Jinan 250022 Shandong Peoples R China

The adjuvant CVC1302 was previously shown to efficiently enhance the immunogenicity of killed footand-mouth disease virus (FMDV) in mice and piglets. However, the underlining mechanism of action of CVC1302 remains unclear, especially at local injection sites and draining lymph nodes. Since the FMDV vaccine is administrated intramuscularly in field settings, we studied local immune responses to FMDV following intramuscular injection in mice, and found that CVC1302-adjuvanted killed FMDV (KV-CVC1302) induced secretion of several chemokines in murine muscle tissues, including MCP-1, MIP-1 alpha, and mIP-1 beta. The number of monocytes recruited to the site of injection was significantly higher in mice immunized with KV-CVC1302 compared with mice immunized with killed FMDV alone (MV). iTAQ-based quantitative proteomic assays were additionally employed to explore the molecular mechanisms of CVC1302 action in the draining lymph nodes. A total of 35 proteins were identified as being differentially expressed among the control group, KV-immunized group and KV-CVC1302-immunized group at 10 days post immunization (dpi). Proteins exhibiting differential expression were mainly involved in signal transduction, apoptosis, endocytosis and innate immune responses. Pathway analysis demonstrated that AMPK, phospholipase D, cAMP, Rapt, and MAPK signaling pathways were potentially induced by the immunopotentiator CVC1302. Understanding the local mechanism of CVC1302 action at injection sites and draining lymph nodes will provide new insights into the development of FMDV vaccines. (C) 2019 Elsevier Ltd. All rights reserved.

关键词： FMDV CVC1302 Local mechanism Injection sites Draining lymph nodes Mice

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[Retracted] Evaluation of Therapeutic Effect and Prognosis of Danzhi Xiaoyao Powder Combined with Photodynamic Therapy in the Treatment of Rose Acne

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Computational and Mathematical Methods in Medicine 2022年第1期2022卷

作者： Xinjian Yu Ni zhang Jing Jin xuwen Yin Jing Luo Hubei Provincial Hospital of Traditional Chinese Medicine Dermatological Department Attending Psychiatrist 430061 *** Hubei Provincial Hospital of Traditional Chinese Medicine Dermatological Department Supervising Technician 430061 ***

Background . Rose acne is a chronic inflammatory skin disease that can cause paroxysmal flushing, persistent erythema, papules or papules on the face, and pustules, and it has a greater impact on the life of patients, so it is important to treat it. Objective . To investigate the effect of Danzhi Xiaoyao Powder combined with photodynamic therapy (PDT) on the curative effect evaluation and prognosis of patients with rose acne. Patients and Methods . The clinical data of 110 rose acne patients who were treated in our hospital from January 2019 to January 2021 were selected as the subject of this retrospective study. They were divided into a control group and a treatment group according to the random residue grouping method. The new crown epidemic, loss to follow-up, etc. fell out of 5 cases in each group, and finally, 50 cases in each group were left. Among them, the control group was treated with PDT, and the treatment group was combined with Danzhi Xiaoyao Powder on the basis of the control group. Then we observe and compare the effects of skin lesion scores and clinical symptom scores and differences in clinical efficacy between the two groups. Results . The comparison of the clinical symptom scores of the two groups of patients before treatment was not statistically significant ( P > 0.05), while the burning score, tingling score, dryness score, and pruritus score of the treatment group after treatment were significantly different. The internal comparison after treatment was lower than before treatment, and the comparison between the treatment groups was significantly higher than the control group, which was statistically significant ( P < 0.05). There was no statistically significant difference in the skin lesion scores of the two groups before treatment ( P > 0.05), while the papules score, pustule score, erythema score, and telangiectasia score of the treatment group after treatment were significantly different and compared within the group. After treatment, th

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地质聚合物多孔材料的制备及应用研究进展

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金属矿山 2022年第10期51卷 226-237页

作者：张凯铭杨浪饶峰方屹宁旭文刘文彪马航福州大学紫金地质与矿业学院福建福州350116 福建省新能源金属绿色提取与高值利用重点实验室福建福州350116 云南云天化股份有限公司研发中心云南昆明650228

地质聚合物多孔材料是一种具有三维结构的无定形多孔胶凝材料,因其优异的物理化学性能及绿色简便的制备方法被认为是未来最具发展潜力的胶凝材料之一。针对国内外地质聚合物多孔材料的最新研究,对地质聚合物多孔材料的制备方法,性能以... 详细信息

地质聚合物多孔材料是一种具有三维结构的无定形多孔胶凝材料,因其优异的物理化学性能及绿色简便的制备方法被认为是未来最具发展潜力的胶凝材料之一。针对国内外地质聚合物多孔材料的最新研究,对地质聚合物多孔材料的制备方法,性能以及相关应用进行了总结,着重阐述了溶剂挥发法、直接发泡法、添加多孔填料法等传统制备方法及其特点,同时还归纳了颗粒堆积法、3D打印法、模板法等新兴的制备方法;在相关的性能和应用研究方面,主要对其抗折压的机械性能,隔热保温的热学性能、对有机无机物的吸附和催化性能、膜分离、电磁波吸收等性能做了总结,指出了其在建筑领域、环境治理领域、电磁屏蔽领域的主要应用场景。论文论述了地质聚合物多孔材料发展现状和瓶颈,指明了当前地聚物多孔材料制备研究中存在成孔方法单一,成孔机理有待深入研究;在性能和应用研究中存在实验室固废试验与实际工业试验偏差大,规模化推广应用难。本文旨在总结最新地质聚合物多孔材料相关制备方法和应用研究,进一步推动地质聚合物多孔材料发展。

关键词：地质聚合物多孔材料制备方法

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切削液中微生物对防锈性影响的研究进展

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材料保护 2024年第11期57卷 73-81页

作者：栗旭文申媛媛刘庆坤张俊庄董丽华董耀华上海海事大学海洋科学与工程学院上海201306 中国石化润滑油有限公司上海研究院上海200080

微生物可导致切削液劣化变质,破坏切削液的防锈性能,进而导致工件和机床的腐蚀。首先概述了切削液中微生物不同的入侵途径,对切削液中微生物种类以及对不同阶段的微生物浓度进行分析,总结了微生物菌种变化规律。其次,从切削液的有效成... 详细信息

微生物可导致切削液劣化变质,破坏切削液的防锈性能,进而导致工件和机床的腐蚀。首先概述了切削液中微生物不同的入侵途径,对切削液中微生物种类以及对不同阶段的微生物浓度进行分析,总结了微生物菌种变化规律。其次,从切削液的有效成分失效、环境pH值的降低和微生物生物膜腐蚀3个方面详细总结了微生物对切削液防锈性的影响。最后,比较分析了目前用于控制和抑制切削液中微生物的方法,并提出参考建议。

关键词：切削液微生物防锈性抑制

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Mapping human tissues with highly multiplexed RNA in situ hybridization

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NATURE COMMUNICATIONS 2024年第1期15卷 2511页

作者： Kalhor, Kian Chen, Chien-Ju Lee, Ho Suk Cai, Matthew Nafisi, Mahsa Que, Richard Palmer, Carter R. Yuan, Yixu zhang, Yida Li, xuwen Song, Jinghui Knoten, Amanda Lake, Blue B. Gaut, Joseph P. Keene, C. Dirk Lein, Ed Kharchenko, Peter V. Chun, Jerold Jain, Sanjay Fan, Jian-Bing zhang, Kun Univ Calif San Diego Dept Bioengn La Jolla CA 92093 USA Univ Calif San Diego Program Bioinformat & Syst Biol La Jolla CA 92093 USA Univ Calif San Diego Dept Elect Engn La Jolla CA USA Sanford Burnham Prebys Med Discovery Inst La Jolla CA USA Univ Calif San Diego Program Biomed Sci La Jolla CA USA Harvard Med Sch Dept Biomed Informat Boston MA USA Altos Labs San Diego CA 94022 USA Washington Univ Sch Med Dept Med St Louis MO USA Washington Univ Sch Med Dept Pathol & Immunol St Louis MO USA Univ Washington Dept Lab Med & Pathol Sch Med Seattle WA USA Allen Inst Brain Sci Seattle WA 98103 USA Illumina San Diego CA USA

In situ transcriptomic techniques promise a holistic view of tissue organization and cell-cell interactions. There has been a surge of multiplexed RNA in situ mapping techniques but their application to human tissues has been limited due to their large size, general lower tissue quality and high autofluorescence. Here we report DART-FISH, a padlock probe-based technology capable of profiling hundreds to thousands of genes in centimeter-sized human tissue sections. We introduce an omni-cell type cytoplasmic stain that substantially improves the segmentation of cell bodies. Our enzyme-free isothermal decoding procedure allows us to image 121 genes in large sections from the human neocortex in <10 h. We successfully recapitulated the cytoarchitecture of 20 neuronal and non-neuronal subclasses. We further performed in situ mapping of 300 genes on a diseased human kidney, profiled >20 healthy and pathological cell states, and identified diseased niches enriched in transcriptionally altered epithelial cells and myofibroblasts.

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