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中华肩肘外科电子杂志 2021年第3期9卷 244-248页

作者：祝相如张聿达李绪文刘国明梁承志扈延龄青岛大学医学部 266073 青岛大学附属医院创伤外科 266000

目的探讨自体髂骨块移植结合Calcar螺钉重建内侧柱治疗肱骨近端骨折的临床疗效。方法回顾性分析我院自2016年1月至2018年12月收治的36例单侧复杂肱骨近端骨折患者,采用自体髂骨块移植结合Calcar螺钉治疗。随访时间12~24个月,平均17.04... 详细信息

目的探讨自体髂骨块移植结合Calcar螺钉重建内侧柱治疗肱骨近端骨折的临床疗效。方法回顾性分析我院自2016年1月至2018年12月收治的36例单侧复杂肱骨近端骨折患者,采用自体髂骨块移植结合Calcar螺钉治疗。随访时间12~24个月,平均17.04个月。记录术后3、6、12个月随访时Neer肩关节功能评分(Neer评分)和肩关节功能评分(Constant评分);采用视觉模拟评分(visual analogue scale,VAS)评估患者术后疼痛程度。分析患者骨折愈合时间、并发症发生情况及肩关节活动范围。结果36例患者均达到解剖复位,骨折平均愈合时间为3.71个月,没有患者出现肱骨头缺血性坏死及内翻塌陷。术后12个月随访时,患者Neer评分(84.64±3.40)分;Constant评分(74.26±4.70)分;VAS(1.24±0.72)分。患肩关节活动度:屈(132.58±8.01)°、伸(43.75±3.41)°、内旋(58.25±4.89)°、体侧外旋(58.89±4.83)°、外展(136.46±11.04)°。结论对于内侧粉碎性复杂肱骨近端骨折,自体髂骨块植骨结合Calcar螺钉能够重建可靠的内侧支撑,降低内翻塌陷及肱骨头缺血性坏死等并发症的风险,术后功能恢复满意。

关键词：肱骨近端骨折髂骨植骨内侧柱骨移植

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三氟拉嗪与八肋游仆虫中心蛋白N端半分子的结合及对蛋白功能的影响

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高等学校化学学报 2019年第11期40卷 2257-2264页

作者：叶旭文张文龙王志军赵亚琴杨斌盛山西大学分子科学研究所化学生物学与分子工程教育部重点实验室

采用荧光光谱、圆二色光谱(CD)、等温滴定量热分析(ITC)、电泳及分子对接等分析技术,研究了三氟拉嗪(TFP)与八肋游仆虫中心蛋白N端半分子(apoN-EoCen)的结合,考察了TFP对apoN-EoCen性质的影响.结果表明,在室温下10 mmol/L Hepes缓冲溶液... 详细信息

采用荧光光谱、圆二色光谱(CD)、等温滴定量热分析(ITC)、电泳及分子对接等分析技术,研究了三氟拉嗪(TFP)与八肋游仆虫中心蛋白N端半分子(apoN-EoCen)的结合,考察了TFP对apoN-EoCen性质的影响.结果表明,在室温下10 mmol/L Hepes缓冲溶液(pH=7.4)中,TFP与apoN-EoCen以摩尔比1∶1结合于apoN-EoCen的第二个EF-手的E,F螺旋之间,条件结合常数约为10^3L/mol;TFP的结合导致蛋白质二级结构发生改变,α螺旋含量减小,Tb^3+敏化荧光强度降低83%,apoN-EoCen切割DNA的类核酸酶活性明显受到抑制;Tb^3+仍可占据复合物apoN-EoCen-TFP中蛋白质的2个金属离子结合位置,条件结合常数约为7.0×10^5L/mol,TFP的结合不抑制金属离子诱导的蛋白质聚集.

关键词： apoN-EoCen 三氟拉嗪 Tb^3+离子光谱分析

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计算机辅助个体化钉道设计在髋臼前柱和耻骨上支骨折螺钉经皮置入中的应用

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精准医学杂志 2022年第2期37卷 161-164页

作者：祝相如陈新张成栋刘巧聪刘国明李绪文王天瑞扈延龄青岛大学附属医院创伤外科山东青岛266100 青岛市城阳区人民医院急诊外科

目的探讨术前计算机辅助个体化钉道设计在髋臼前柱和耻骨上支骨折螺钉经皮置入术中的临床应用价值。方法回顾性分析我院2018年12月—2020年12月40例(共45侧)使用通道螺钉技术进行治疗的髋臼前柱或耻骨上支骨折患者。根据术前是否使用计... 详细信息

目的探讨术前计算机辅助个体化钉道设计在髋臼前柱和耻骨上支骨折螺钉经皮置入术中的临床应用价值。方法回顾性分析我院2018年12月—2020年12月40例(共45侧)使用通道螺钉技术进行治疗的髋臼前柱或耻骨上支骨折患者。根据术前是否使用计算机进行个体化钉道设计分为计算机辅助组(A组)和常规手法组(B组),A组患者术前均使用Mimics软件进行螺钉预置入,确定螺钉入钉点、螺钉方向和螺钉长度。B组按照手术操作者经验及以往解剖学研究得出的螺钉角度进行螺钉置入,不进行个体化设计。记录两组患者术中每枚螺钉的置入时间(置钉时间)、术中透视次数(透视次数)、螺钉长度、骨折愈合时间和术后6个月随访时的髋关节Merle D′Aubignéand Postel评分与骨盆Majeed功能评分。结果A组患者置钉成功率高于B组(χ^(2)=8.666,P<0.05),A组置钉时间、透视次数较B组明显减少(t=5.910、8.138,P<0.05),A组螺钉长度较B组明显缩短(t=18.863,P<0.05),两组患者骨折愈合时间及术后6个月时功能评分比较差异无显著性(P>0.05)。结论术前使用计算机对髋臼前柱和耻骨上支螺钉位置进行个体化设计,能够在保证手术效果的前提下缩短螺钉长度,从而降低拉力螺钉置入难度,能够为无或轻度移位的髋臼前柱和耻骨上支骨折的治疗提供新的方法,且临床疗效满意。

关键词：髋臼耻骨骨折,闭合性骨折固定术,内骨螺丝拉力螺钉计算机辅助设计

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CTA1: Purified and display onto gram-positive enhancer matrix (GEM) particles as mucosal adjuvant

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PROTEIN EXPRESSION AND PURIFICATION 2018年 141卷 19-24页

作者： zhang, Yuanpeng Yu, Xiaoming Hou, Liting Chen, Jin Li, Pengcheng Qiao, xuwen Zheng, Qisheng Hou, Jibo Jiangsu Acad Agr Sci Inst Vet Immunol & Engn Nanjing 210014 Jiangsu Peoples R China Jiangsu Acad Agr Sci Natl Res Ctr Engn & Technol Vet Biol 50 Zhongling St Nanjing 210014 Jiangsu Peoples R China Jiangsu Coinnovat Ctr Prevent & Control Important Yangzhou 225009 Jiangsu Peoples R China

The A1 subunit of cholera toxin (CTA1) retains the adjuvant function of CT, without its toxic side effects, making the molecule a promising mucosal adjuvant. However, the methods required to obtain a pure product are both complicated and expensive, constricting its potential commercial applicability. Here, we fused the peptidoglycan binding domain (PA) to the C-terminus of CTA1, which enabled the fusion protein to be expressed by Bacillus subtilis, and secreted into the culture medium. CTA1 was then purified and displayed on GEM particles using a one step process, which resulted in the formation of CTA1-GEM complexes. Next, the CTA1-GEM complexes were used as an adjuvant to enhance the immune responses of mice to the influenza subunit vaccine. It was observed that the CTA1-GEM complexes enhanced specific systemic (IgG) and mucosal (IgA) immune responses against antigen, and induced cellular immune responses as well. The data presented here suggests that CTA1-GEM complexes can serve as a viable mucosal adjuvant. (C) 2017 Elsevier Inc. All rights reserved.

关键词： Cholera toxin A1 subunit Surface display Adjuvant GEM particles Lactococcus lactis

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Analysis of heart rate variation and foetal quiet sleep cycle correlation for normal and suspicious foetuses

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BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 2018年第4期32卷 988-992页

作者： Li, Guangfei zhang, Song Yang, Lin Li, Shufang Wang, Yan Zhao, Yangyu Yang, Yimin Hao, Dongmei Li, xuwen zhang, Lei Xu, Mingzhou Beijing Univ Technol Coll Life Sci & Bioengn Beijing Peoples R China Peking Univ Hosp 3 Obstet & Gynecol Beijing Peoples R China Beijing Yes Med Devices Co Ltd Beijing Peoples R China Beijing Aerosp Changfeng Co Ltd Beijing Peoples R China

Cardiotocography (CTG) is the basic method of monitoring foetal condition in the perinatal period. However, due to the limited insight into the CTG generation mechanism, some CTG recordings can be mistreated as a predictor of neonatal asphyxia, leading to a wrong assessment of the foetal status and improper obstetric intervention. Therefore, in case of suspicious CTG indications, a reliable verification of the true foetal status is vital for reducing the clinical diagnosis false-positive rate and avoiding the unnecessary obstetrician intervention. The paper presents the results obtained for 44 foetuses on the shares of three heart rate variation (HRV) ranges and quiet sleep cycles. This approach uses the parameters of the HRV range shares and quiet sleep cycles to analyse different types of foetuses. The results obtained show that most parameters reveal significant differences between normal and suspicious groups. This is quite lucrative for defining a set of coefficients, whose incorporation into a classifier will improve the accuracy of distinguishing the normal foetuses from suspicious ones.

关键词： Foetal heart rate foetal quiet sleep HRV range share

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CollAFL: Path Sensitive Fuzzing 39

CollAFL: Path Sensitive Fuzzing

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39th IEEE Symposium on Security and Privacy (SP)

作者： Gan, Shuitao zhang, Chao Qin, Xiaojun Tu, xuwen Li, Kang Pei, Zhongyu Chen, Zuoning State Key Lab Math Engn & Adv Comp Zhengzhou Henan Peoples R China Tsinghua Univ Inst Network Sci & Cyberspace Beijing Peoples R China Cyber Immun Lab Scottsdale AZ USA Natl Res Ctr Parallel Comp Engn & Technol Beijing Peoples R China

ISBN: (纸本)9781538643532

Coverage-guided fuzzing is a widely used and effective solution to find software vulnerabilities. Tracking code coverage and utilizing it to guide fuzzing are crucial to coverage-guided fuzzers. However, tracking full and accurate path coverage is infeasible in practice due to the high instrumentation overhead. Popular fuzzers (e.g., AFL) often use coarse coverage information, e.g., edge hit counts stored in a compact bitmap, to achieve highly efficient greybox testing. Such inaccuracy and incompleteness in coverage introduce serious limitations to fuzzers. First, it causes path collisions, which prevent fuzzers from discovering potential paths that lead to new crashes. More importantly, it prevents fuzzers from making wise decisions on fuzzing strategies. In this paper, we propose a coverage sensitive fuzzing solution CollAFL. It mitigates path collisions by providing more accurate coverage information, while still preserving low instrumentation overhead. It also utilizes the coverage information to apply three new fuzzing strategies, promoting the speed of discovering new paths and vulnerabilities. We implemented a prototype of CollAFL based on the popular fuzzer AFL and evaluated it on 24 popular applications. The results showed that path collisions are common, i.e., up to 75% of edges could collide with others in some applications, and CollAFL could reduce the edge collision ratio to nearly zero. Moreover, armed with the three fuzzing strategies, CollAFL outperforms AFL in terms of both code coverage and vulnerability discovery. On average, CollAFL covered 20% more program paths, found 320% more unique crashes and 260% more bugs than AFL in 200 hours. In total, CollAFL found 157 new security bugs with 95 new CVEs assigned.

关键词： Fuzzing Security Instruments Computer bugs Grammar Target tracking"

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FilterLAP: Filtering False-positive Mutation Calls via a Label Propagation Framework

FilterLAP: Filtering False-positive Mutation Calls via a Lab...

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IEEE International Conference on Bioinformatics and Biomedicine

作者： xuwen Wang Xiaoyan Zhu Xiao Xiao Shenjie Wang Xuanping zhang Xin Lai Jiayin Wang Shaanxi Engineering Research Center of Medical and Health Big Data Xi'an Jiaotong UniversityXi'anShaanxiChina710049 School of Pub

ISBN: (纸本)9781728118680

Benefiting from the recent advantages of genomic sequencing, detecting genomic mutations becomes a routine work in precise diagnoses and treatments for cancers. In clinical practices, many factors, such as tumor purity, clonal structure, etc., interfere the performance of calling mutations. The computational pipelines prefer to sensitively report the candidate calls, while a filter is applied for removing the false-positive calls. The existing filters rely on the whole genome/exome sequencing data, which can provide sufficient samples for training the filters. However, the gene-panel sequencing is more popular in clinical practices, but there is no practical filter for limited training samples. In light of this, we develop a semi-learning filter for gene-panel sequencing data, FilterLAP, which implemented via a label propagation framework. Given few labeled samples with a set of unlabeled ones, its basic idea is to predict the label information of unlabeled nodes from the label information of labeled nodes, and establishes a complete graph model by using the relationship between samples, by combining transductive inference with label propagation algorithm. For each node in the network, tags are propagated to adjacent nodes according to similarity and the probability distribution of similar nodes tends to be similar and can be divided into a class. We perform multiple sets of experiments on gene-panel sequencing data captured from Illumina platform. FilterLAP outperforms on both SNV and INDEL filtering, where the AUCs reach 0.90-0.97, and the average accuracies on overall mutation calls are over 90%. Comparing to GATK hard filters, FilterLAP present a 5% improvement on accuracy. These results demonstrate that the proposed method can better reduce the false positive mutation calls on gene-panel sequencing data. In addition, it is stable and efficient, which can be used as a practical tool for mutation call filtering for gene-panel sequencing data.

关键词： cancer data analysis genetics genomics graph theory inference mechanisms learning (artificial intelligence) medical diagnostic computing probability medical diagnostic computing Inference mechanisms Genomics Graph theory calls Filters Data analysis Analyses, Sequence Genetics Probability Filtration adjacent nodes Probability distribution

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CNTs/CNFs/GCE电化学传感器的制备及其对木犀草素的检测

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广西科技大学学报 2025年第2期36卷 118-127页

作者：朱旭文郭双李春婷张丽文程昊唐婷范广西糖资源绿色加工重点实验室(广西科技大学) 广西柳州545006 广西柳州螺蛳粉工程技术研究中心(广西科技大学) 广西柳州545006 广西科技大学生物与化学工程学院广西柳州545006 杭州微念品牌管理有限公司浙江杭州310000

以碳纳米管(carbon nanotubes,CNTs)和聚丙烯腈(polyacrylonitrile,PAN)为前驱体,通过静电纺丝得到纳米纤维,后经碳化得到CNTs/CNFs复合材料。使用扫描电子显微镜(scanning electron microscope,SEM)、X射线衍射(X-ray diffraction,XRD)、能量色谱仪(energy dispersive spectroscopy,EDS)和电化学阻抗谱(electrochemical impedance spectroscopy,EIS)对其形貌和组成进行了表征;以滴涂法将此材料修饰到玻碳电极(glassy carbon electrode,GCE)表面,考察木犀草素在该复合电极上的电化学行为;最后将此修饰电极应用到金银花露中木犀草素的测定。结果表明:此修饰电极在检测木犀草素的试验中展现出良好的重复性、重现性和抗干扰能力;当木犀草素浓度在3~10μmol/L时,CNTs/CNFs/GCE修饰电极响应电流值与木犀草素浓度呈良好的线性关系,检测限为0.18μmol/L;用此复合电极检测金银花露中的木犀草素,加标回收率在96.069%~97.623%之间,相对标准偏差小于5.0%。

关键词：木犀草素碳纳米管静电纺丝法电化学加标回收率

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Factors associated with blooms of cyanobacteria in a large shallow lake, China

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ENVIRONMENTAL SCIENCES EUROPE 2018年第1期30卷 1-15页

作者： Li, Di Wu, Naicheng Tang, Song Su, Guanyong Li, xuwen zhang, Yong Wang, Guoxiang zhang, Junyi Liu, Hongling Hecker, Markus Giesy, John P. Yu, Hongxia Nanjing Univ Sch Environm State Key Lab Pollut Control & Resource Reuse Nanjing 210046 Jiangsu Peoples R China Jiangsu Environm Monitoring Ctr Nanjing 210036 Jiangsu Peoples R China Univ Kiel Dept Hydrol & Water Resources Management D-24118 Kiel Germany Chinese Ctr Dis Control & Prevent Natl Inst Environm Hlth Beijing 100021 Peoples R China Nanjing Univ Sci & Technol Sch Environm & Biol Engn Jiangsu Key Lab Chem Pollut Control & Resources R Nanjing 210094 Jiangsu Peoples R China Nanjing Normal Univ Sch Environm Nanjing 210023 Jiangsu Peoples R China Wuxi Environm Monitoring Ctr Wuxi 214000 Jiangsu Peoples R China Univ Saskatchewan Toxicol Ctr Saskatoon SK S7N 5B3 Canada Univ Saskatchewan Sch Environm & Sustainabil Saskatoon SK S7N 5C3 Canada Univ Saskatchewan Dept Vet Biomed Sci Saskatoon SK S7N 5B3 Canada Univ Saskatchewan Toxicol Ctr Saskatoon SK S7N 5B3 Canada Michigan State Univ Dept Zool E Lansing MI 48824 USA Michigan State Univ Ctr Integrat Toxicol E Lansing MI 48824 USA Univ Hong Kong Sch Biol Sci Hong Kong Hong Kong Peoples R China

Background: Eutrophication of freshwater systems can result in blooms of phytoplankton, in many cases cyanobacteria. This can lead to shifts in structure and functions of phytoplankton communities adversely affecting the quality of drinking water sources, which in turn impairs public health. Relationships between structures of phytoplankton communities and concentrations of the toxicant, microcystin-leucine-arginine (MC-LR), have not been well examined in large shallow lakes. The present study investigated phytoplankton communities at seven locations from January to December of 2015 in Tai Lake, and relationships between structures and diversities of phytoplankton communities and water quality parameters, including concentrations of MC-LR and metals, were analyzed. Results: A total of 124 taxa of phytoplankton were observed, and the predominant taxa were Microcystis sp. and Dolichospermum flos-aquae of Cyanophyta and Planctonema sp. of Chlorophyta. The greatest diversities of phytoplankton communities, as indicated by species richness, Simpson, Shannon-Wiener, the Berger and Parker, and the Pielou evenness indices, were observed in spring. Furthermore, productivity of phytoplankton was significantly and negatively correlated with diversities. These results demonstrated that Simpson, Shannon-Wiener, the Berger and Parker, and the Pielou evenness indices of phytoplankton communities were significantly related to trophic status and overall primary productivity in Tai Lake. In addition, temperature of surface water, pH, permanganate index, biochemical oxygen demand, total phosphorus, arsenic, total nitrogen/total phosphorous ratio, and MC-LR were the main factors associated with structures of phytoplankton communities in Tai Lake. Conclusion: The present study provided helpful information on phytoplankton community structure and diversity in Tai Lake from January to December of 2015. Our findings demonstrated that Simpson, Shannon-Wiener, the Berger and Parker, and the

关键词： Eutrophication Nutrients Phosphorus Nitrogen Phytoplankton productivity Diversity Microcystin-LR

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球磨工艺对铜基摩擦材料混合料的影响Ⅰ:对粒度分布的影响

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粉末冶金技术 2016年第6期34卷 444-449页

作者：殷德政石增敏张大勇刘旭文三峡大学材料与化工学院湖北宜昌443000 天津瑞克曼德科技发展有限公司天津300050

采用单因素试验方法,研究了行星式球磨工艺参数如球料比、球磨时间、磨球直径、醇料比等对铜基粉末冶金摩擦材料混合料粒度分布的影响规律。结果表明,随球料比的增加,混合料的平均粒径不断减小,球料比为15∶1时,混合料的平均粒径最小,为... 详细信息

采用单因素试验方法,研究了行星式球磨工艺参数如球料比、球磨时间、磨球直径、醇料比等对铜基粉末冶金摩擦材料混合料粒度分布的影响规律。结果表明,随球料比的增加,混合料的平均粒径不断减小,球料比为15∶1时,混合料的平均粒径最小,为1.345μm;球磨时间对混合料平均粒径的影响规律是随着球磨时间的增加混合料平均粒径先减小后增大;一定范围内的醇料比在湿磨过程中对研磨效率影响不大;相比于刚玉磨球,钢质磨球对于混合料的研磨效率更好。

关键词：铜基摩擦材料粉末冶金球磨工艺粒度分布

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