Food allergy has become a worldwide problem. To find an effective way to reduce the allergic effect of allergen is an interesting topic. In this work, the interaction between ovalbumin（OVA） and tangeretin was studie...
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Food allergy has become a worldwide problem. To find an effective way to reduce the allergic effect of allergen is an interesting topic. In this work, the interaction between ovalbumin（OVA） and tangeretin was studied by means of spectroscopy, molecular docking calculation and animal experiment. As the results show, static quenching of fluorescence intensity happened due to the interaction between OVA and tangeretin. Binding constants, binding sites, and thermometric parameters were obtained. The conformation changing of OVA occurred due to the interaction between it and tangeretin. The molecular docking calculation results show that the epitopes Arginine 84（ARG84）, Leucine 87（LEU87）, Asparagine 88（ASN88）, Serine 103（SER103）, Arginine 104（ARG104）, Leucine 124（LEUI24）, Arginine 126（ARG126）, Glycine 127（GLY127）, Glycine 128（GLY128） and Tryptohan 148（TRP148） of OVA were occupied by tangeretin after the docking, which means tangeretin may have some inhibition effects. As the results of animal experiments, the amount of IgE and lung tissue sections of female BALB/c mice in different groups show that tangeretin with different concentrations has better effects than dexamethasone. In conclusion, the tangeretin may have the effects to reduce the allergic effects of OVA via occupying the IgE-binding epitopes of OVA.
BackgroundThe aim of this study was to investigate the effect of bronchopulmonary dysplasia (BPD) on the early intellectual development of preterm infants. MethodsFrom 2011 to 2015, 83 preterm infants diagnosed with B...
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BackgroundThe aim of this study was to investigate the effect of bronchopulmonary dysplasia (BPD) on the early intellectual development of preterm infants. MethodsFrom 2011 to 2015, 83 preterm infants diagnosed with BPD were recruited to the BPD group, and 89 preterm infants without BPD and 98 healthy term infants were randomly recruited to the non-BPD and term group, respectively. Neural and intellectual development according to the Gesell Development Scale were evaluated and compared between groups at 0-3 months, 3-6 months, 6-9 months, and 9-12 months of adjusted age for preterm infants and real age for term infants. Multivariate logistic regression was used to determine the associations between BPD and adverse neurological outcomes at 9-12 months of adjusted age. ResultsCompared with term infants, preterm infants had significantly lower developmental quotients for adaptability, gross motor, fine motor, language and social skills. At follow up, deficits in one or more neurofunctions related to adaptability, gross motor, fine motor, language and social skills were significantly more frequent in preterm children with BPD than in those with no history of BPD. BPD was independently associated with adverse neurological outcome at 9-12 months of adjusted age in preterm infants. ConclusionsEarly intelligence disturbances occurred significantly more frequently in BPD infants than in non-BPD infants. Monitoring of the development of the nervous system in BPD infants should be strengthened.
A vertical cavity surface emitting laser (VCSEL) device, comprising: a bracket that has a hollow cavity (2) and a VCSEL chip (1) encapsulated in the hollow cavity (2), wherein the bracket comprises a base (3) and a di...
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WO2019/165769(A1)
A vertical cavity surface emitting laser (VCSEL) device, comprising: a bracket that has a hollow cavity (2) and a VCSEL chip (1) encapsulated in the hollow cavity (2), wherein the bracket comprises a base (3) and a diffusion sheet (4) used for sealing the base (3), the VCSEL chip (1) is encapsulated on the base (3), and the diffusion sheet (4) is disposed above a light emergent surface of the VCSEL chip (1), such that light emitted from the VCSEL chip (1) traverses the diffusion sheet (4) and is emitted to the outside;a cut-off film (5) is disposed on the diffusion sheet (4), a through hole (6) is opened on the cut-off film (5), and the through hole (6) is disposed above the light emergent surface of the VCSEL chip (1), such that the light emitted from the VCSEL chip (1) penetrates the through hole (6), and then traverses the diffusion sheet (4) and is emitted to the outside. The flexible adjustment of the light-emitting angle is achieved by means of combining the VCSEL chip (1) with the diffusion sheet (4). Additionally, by means of disposing the cut-off film (5) on the diffusion sheet (4), the light scattered on the cut-off film (5) is reflected back outside of the VCSEL device, thereby improving the light emergence effect.
On the basis of fluorescence resonance energy transfer (FRET), a new rhodamine derivative (DRh) was synthesized as a ratiometric fluorescent probe for detecting Hg2+ in water and living cells samples. The recognition ...
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On the basis of fluorescence resonance energy transfer (FRET), a new rhodamine derivative (DRh) was synthesized as a ratiometric fluorescent probe for detecting Hg2+ in water and living cells samples. The recognition properties of the probe DRh with metal ions had been investigated in H2O/CH3CN (9:1, v/v;Tris-HCl 50 mmol L-1;pH = 7.0) solution by the UV-Vis spectrophotometry and the fluorescence spectrophotometry. The results showed that the probe DRh exhibited the selective recognition of Hg2+. Upon the addition of Hg2+, the spirolactam ring of probe DRh was opened. The 1:1 stoichiometric structure between DRh and Hg2+ were supported by Job's plot, MS and OFT theoretical calculations. The linearly fluorescence intensity ratio (I-582/I-538) is proportional to the concentration of Hg2+ in the range 0-30 mu mol L-1. The limit of detection (LOD) of Hg2+ is 0.008 mu mol L-1 (base on S/N = 3). The present probe was applied to the determination of Hg2+ in neutral water samples and gave recoveries ranging from 104.5 to 107.9%. Furthermore, the fluorescent probe also can be applied as a bioimaging reagent for Hg2+ detection in HeLa cells. (C) 2016 Elsevier B.V. All rights reserved.
A rapid and effective sample pretreatment method, dispersant-assisted dynamic microwave extraction, was first applied to the extraction of six triazine herbicides from rice. The dynamic microwave extraction was couple...
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A rapid and effective sample pretreatment method, dispersant-assisted dynamic microwave extraction, was first applied to the extraction of six triazine herbicides from rice. The dynamic microwave extraction was coupled with matrix solid-phase dispersion (MSPD). The dispersant in MSPD was used to disperse the sample and adsorb impurities. Microwave irradiation was used to promote the transfer of the analyte and accelerate the extraction. The extraction and clean-up were performed simultaneously. The effects of experimental parameters, including the solvent type, volume of the extraction solvent, microwave power, type of dispersant, mass ratio of dispersant to rice, and flow rate of the extraction solvent, on the recoveries of the analytes were investigated. The limits of detection for the analytes ranged from 0.62 to 1.79 mu g kg(-1). The recoveries of the triazine herbicides in spiked rice samples were in the range of 84.3-101%, and the relative standard deviations were lower than 6.2%.
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