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Preparation of CeO2 by ion-exchange membrane electrolysis method

HYDROMETALLURGY 2019年 186卷 126-131页

作者： Meng, Deliang Zhao, Qiuyue Pan, Xijuan Lv, Guozhi Zhang, Ting'an Northeastern Univ Minist Educ Sch Met Key Lab Ecol Met Multimet Intergrown Ores Shenyang 110819 Liaoning Peoples R China

The method of ionic membrane electrolysis was applied to prepare cerium (IV) oxide from cerium (III) chloride aqueous solution. This research focused on the effect of different current densities on electrolytic product. The results indicated that the method of ion-exchange membrane electrolysis was feasible to produce pure CeO2 particles with the chlorine content of lower than 0.020%. Under the conditions of this study, when the current density changed from 100 ***(-2) to 400 mAcm(-2), the current efficiency of CeO2 decreased monotonically from about 89.0% to 75.0%, the median particle sizes (D-50) of CeO2 samples were about 9 mu m similar to 24 mu m. After 1.5 h of roasting at 800 degrees C, the crystals of CeO2 continued to grow, and the roasted product was still CeO2, whose particle size of D50 increased from about 17 mu m (before roasting) to 27 mu m (after roasting). Additionally, it was obvious that the crystal after roasting was composed of nano-sized spherical particles.

关键词： Cerium (III) chloride Ion-exchange membrane electrolysis Cerium (IV) oxide Current efficiency

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Identification and characterization of a new long noncoding RNA iab-1 in the Hox cluster of silkworm, Bombyx mori identification of iab-1

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JOURNAL OF CELLULAR BIOCHEMISTRY 2019年第10期120卷 17283-17292页

作者： Wang, Honglei Hu, Hai Xiang, Zhonghuai Lu, Cheng Dai, Fangyin Tong, Xiaoling Southwest Univ State Key Lab Silkworm Genome Biol Key Lab Sericultural Biol & Genet Breeding Minist Agr Chongqing 400715 Peoples R China

Long noncoding RNAs (lncRNAs) areinvolvedin a variety of biological processes. In silkworm, numerous lncRNAs have been predicted through deep transcriptome sequencing, but no functional role has been experimentally validated yet. Here, we characterized a new lncRNA iab-1 that was mainly encoded by the intergenic region between Bmabd-A and Bmabd-B in the Homeobox (Hox) cluster of the silkworm, Bombyx mori. More than seven alternative splicing isoforms of lncRNA iab-1 were cloned, which were subgrouped into types 1 and 2 based on the location of the 3 '-ends. The iab-1 was expressed at a low level, but the expression of iab-1 peaked at several specific development stages, including 3 to 4 days during the embryonic stage, stages before fourth molting, and the sixth hour after the fourth molting, and early stages during metamorphosis. It was highly expressed in the nervus and epidermis, especially the epidermis of the posterior abdomen at the fourth instar premolting stage. The relationship between iab-1 and nearby Hox genes was analyzed at different developmental stages. Iab-1 expression was highly associated with Bmabd-A as well as Bmabd-B in the embryonic and larval stages, while this association was decreased at the metamorphic stage;iab-1 expression was highly associated with BmUbx only in the embryonic stage. Downregulation of iab-1 expression by small interfering RNA led to the death of most of the treated individuals at the larval stage, suggesting that iab-1 transcript expression might be involved in certain relevant physiological processes. The expression of Bmabd-A and Bmabd-B did not change in iab-1 downregulated individuals, indicating that the relevance between the two genes and iab-1 was not induced by iab-1 transcript. Collectively, the results showed that the newly identified iab-1 may be involved in some physiological processes, and the interaction between iab-1 and Hox genes was also preliminarily analyzed.

关键词： Hox iab-1 insects lncRNA silkworm

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3D-Printed Sensor for Unshielded Scalar Magnetometry Based on Nonlinear Magneto-Optical Rotation with Amplitude Modulated Light

3D-Printed Sensor for Unshielded Scalar Magnetometry Based o...

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Joint Conference of the IEEE International Frequency Control Symposium / Conference of the European-Frequency-and-Time-Forum (EFTF/IFC)

作者： Zhang, Rui Pang, Bo Ding, Yudong Li, Wenhao Chen, Jingbiao Peng, Xiang Guo, Hong Peking Univ State Key Lab Adv Opt Commun Syst & Network Sch Elect Engn & Comp Sci Beijing 100871 Peoples R China Peking Univ Ctr Quantum Informat Technol Beijing 100871 Peoples R China Natl Univ Def Technol Coll Liberal Arts & Sci Changsha 410073 Peoples R China Natl Univ Def Technol Interdisciplinary Ctr Quantum Informat Changsha 410073 Peoples R China

In this article, we build and test a 3D-printed sensor for a scalar atomic magnetometer. The sensor works well and reaches a power spectral density of 0.1 pT/root Hz in an unshielded environment. The design freedom an... 详细信息

ISBN: (纸本)9781538683057

关键词： 3D-printing atomic magnetometer sensor

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The beta-1,4-N-acetylglucosaminidase 1 gene,selected by domestication and breeding,is involved in cocoon construction of Bombyx mori

The beta-1,4-N-acetylglucosaminidase 1 gene,selected by dome...

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中国蚕学会2021年学术年会

作者： Chunlin Li Xiaoling Tong Weidong Zuo Hai Hu Gao Xiong Minjin Han Rui Gao Yue Luan Kunpeng Lu Tingting Gai Zhonghuai Xiang Cheng Lu Fangyin Dai State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericultural Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of BiotechnologySouthwest University

Holometabolous insects have distinct larval,pupal,and adult *** pupal stage is typically immobile and can be subject to predation,but cocoon offers pupal protection for many insect *** cocoon provides a space in which the pupa to adult metamorphosis *** also protects the pupa from weather,predators and *** protein is a precursor of the silk used in cocoon *** used the silkworm as a model species to identify genes affecting silk protein synthesis and cocoon *** used quantitative genetic analysis to demonstrate that-1,4-N-acetylglucosaminidase 1(BmGlcNase1) is associated with synthesis of sericin,the main composite of ***1 has an expression pattern coupled with silk gland development and cocoon shell weight(CSW) variation,and CSW is an index of the ability to synthesize silk ***-regulated expression of BmGlcNase1 increased sericin content by 13.9% and 22.5% while down-regulation reduced sericin content by 41.2% and 27.3% in the cocoons of females and males,*** sequencing revealed that sequence variation upstream of the BmGlcNase1 transcriptional start site(TSS) is associated with the expression of BmGlcNase1 and *** pressure analysis showed that GlcNase1 was differentially selected in insects with and without cocoons(ω1=0.044 vs.ω2=0.154).This indicates that this gene has a conserved function in the cocooning process of ***1 appears to be involved in sericin synthesis and silkworm cocooning.

关键词： The beta-1,4-N-acetylglucosaminidase 1 gene,selected by domestication and breeding,is involved in cocoon construction of Bombyx mori

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Amplitude Dependence on Probe Frequency Detuning in Faraday-rotation Alkali Atomic Magnetometers

Amplitude Dependence on Probe Frequency Detuning in Faraday-...

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Joint Conference of the IEEE International Frequency Control Symposium / Conference of the European-Frequency-and-Time-Forum (EFTF/IFC)

作者： Yang, Yucheng Chen, Jingbiao Peng, Xiang Guo, Hong Peking Univ State Key Lab Adv Opt Commun Syst & Network Sch Elect Engn & Comp Sci Beijing 100871 Peoples R China Peking Univ Ctr Quantum Informat Technol Beijing 100871 Peoples R China

In this article, the dependence of the magnetic resonance signal amplitude on the frequency detuning of probe light in Faraday-rotation alkali atomic magnetometers is quantitatively studied. The sensitivity of atomic ... 详细信息

ISBN: (纸本)9781538683057

关键词： Faraday-rotation magnetometer probe frequency detuning alkali atoms transition strength

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Identification,expression,and artificial selection of silkworm epigenetic modification enzymes

Identification,expression,and artificial selection of silkwo...

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中国蚕学会2021年学术年会

作者： Rui Gao Chun-Lin Li Xiao-Ling Tong Min-Jin Han Kun-Peng Lu Shu-Bo Liang Hai Hu Yue Luan Bi-Li Zhang Yan-Yu Liu Fang-Yin Dai State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericultural Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of BiotechnologySouthwest University

Background:Understanding the genetic basis of phenotype variations during domestication and breeding is of great *** and epigenetic modification enzymes(EMEs) may play a role in phenotypic variations;however,no comprehensive study has been performed to *** silkworm(Bombyx mori) may be utilized as a model in determining how EMEs influence domestication traits.

关键词： Identification,expression,and artificial selection of silkworm epigenetic modification enzymes

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hippo pathway regulates somatic development and cell proliferation of silkworm

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GENOMICS 2019年第3期111卷 391-397页

作者： Li, Niannian Tong, Xiaoling Zeng, Jie Meng, Gang Sun, Fuze Hu, Hai Song, Jiangbo Lu, Cheng Dai, Fangyin Southwest Univ State Key Lab Silkworm Genome Biol Key Lab Sericulture Funct Genom & Biotechnol Agr Minist Chongqing 400715 Peoples R China

hippo signaling pathway (signaling pathway hippo, hereinafter referred to as the hippo pathway) was the earliest found in Drosophila (Schneck [1]), which can regulate the development of tissues and organs, even some components of the pathway were identified as tumor suppressor [2]. The pathway was more concerned in fruit flies and mice (Schneck [1]), but little attention has been given in silkworm, an important economic and lepidopteran model insect. In this manuscript, we identified major hippo pathway related genes (hippo, Salvador, Warts, Mats, Yorkie) in silkworm and named BmHpo, BmSav, BmWts, BmMats, BmYki. The domain organization of these genes was highly conserved in silkworm and other organisms suggesting that they could use similar protein-protein interactions to construct the hippo kinase cascades. The expression profiles of these genes in silkworm during embryonic, larval, wandering, pupal and adult stages were analyzed, 14 tissues/organs of the day 3, 5th instar larvae (L5D3) as well. Experimental results showed that the expression of hippo pathway had some influence on the development of silkworm. In order to find out the mechanism of hippo pathway affecting silkworm development, BmHpo and BmYki were up-regulated and de-regulated in the cell line of Bombyx mori-BmN-SWU1(NS), and the changes of cell proliferation activity and cell cycle were detected. The distribution of BmYki was detected by immunofluorescence technique. This study provides insights into the genes of hippo pathway which have a certain effect on somatic development and cell proliferation in silkworm.

关键词： hippo pathway development Cell proliferation Cell cycle Silkworm

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Ara-c induces cell cycle G1/S arrest by inducing upregulation of the INK4 family gene or directly inhibiting the formation of the cell cycle-dependent complex CDK4/cyclin D1

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CELL CYCLE 2019年第18期18卷 2293-2306页

作者： Sun, Fuze Li, Niannian Tong, Xiaoling Zeng, Jie He, Songzhen Gai, Tingting Bai, Yanmin Liu, Lanlan Lu, Kunpeng Shen, Jianghong Han, Minjin Lu, Cheng Dai, Fangyin Southwest Univ Coll Biotechnol Minist Agr State Key Lab Silkworm Genome BiolKey Lab Sericu Chongqing Peoples R China

Cytosine arabinoside (Ara-c) is a pyrimidine anti-metabolite that is capable of interfering with cellular proliferation by inhibiting DNA synthesis. Each inhibitor of cyclin-dependent kinase 4 (INK4) family member has the ability to bind to cyclin-dependent kinase 4 (CDK4) and inhibit the formation of the cell cycle-dependent CDK4/cyclin D1 complex, subsequently leading to cell cycle arrest in the G1/S phase. In this study, the expression of INK4 family genes in kidney cancer and the impact of these genes on patient prognosis were examined. Additionally, the effects of INK4 family genes and Ara-c on cell proliferation and tumor formation and development were examined. Finally, a potential association between Ara-c-induced cell cycle arrest and INK4-associated gene expression was evaluated. An upregulation of INK4 family genes was found to be positively correlated with the prognosis of patients with kidney cancer. Both the INK4 family genes and Ara-c were shown to induce cell cycle arrest and inhibit tumor formation and development. Moreover, Ara-c-induced cell cycle arrest was found to be associated with an Ara-c-induced upregulation of INK4 family gene expression, which ultimately inhibited the formation of the CDK4/cyclin D1 complex. These findings suggested that an upregulation of INK4 family genes has a positive effect on kidney cancer prognosis and can inhibit the formation and development of tumors. Moreover, Ara-c was shown to promote the upregulation of INK4 family genes, at the same time, Ara-c could directly regulate the cell cycle-dependent genes CDK4 and cyclin D1 (CCND1), independent of the INK4 family genes.

关键词： INK4 family Ara-c cell proliferation cell cycle CDK4 Cyclin D1

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BE-PIGS: a base-editing tool with deaminases inlaid into Cas9 PI domain significantly expanded the editing scope

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Signal Transduction and Targeted Therapy 2019年第1期4卷 327-329页

作者： Yanhong Wang Lifang Zhou Nan Liu Shaohua Yao State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan University610041 Chengdu People’s Republic of China

Dear Editor,The discovery of base-editing tools sheds new light on both basic biomedical research and clinical therapeutics.1,2 Although showing profound performance in a wide range of applications,current base editors have their *** particular,the narrow editing windows of these base editors have significantly limited their targetable *** current base editors,the most frequent way to tether deaminase is to fuse it to the N-terminus of the Cas9 protein through flexible peptide *** editors derived from Streptococcus pyogenes Cas9(SpyCas9)or its variants can efficiently edit desired bases in an~5 nt wide window within the detached,non-target strand(NTS).Maximum editing typically occurs around positions 5–7,counting from the 5′end of the protospacer,which is about 15 nt away from the protospacer adjacent motif(PAM).

关键词： base expanded editing

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Preparation of La2O3 by ion-exchange membrane electrolysis of LaCl3 aqueous solution

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Journal of Rare Earths 2019年第9期37卷 1009-1014,I0005页

作者： Deliang Meng Qiuyue Zhao Xijuan Pan Ting’an Zhang Key Laboratory of Ecological Metallurgy of Multi-Metal Intergrown Ores of Ministry of Education School of MetallurgyNortheastern University

The ion-exchange membrane electrolysis technology was applied inventively in the preparation of lanthanum hydroxide,after calcination,lanthanum oxide was obtained *** effect of the concentration of lanthanum chlo ride solution on electrolytic product was mainly *** phase composition,micro morphology,particle size and chlorine content of the product were characterized,*** the conditions of this work,the product that forms by electrolysis of LaCl3 aqueous solution directly is La(OH)*** the initial concentration of LaCl3 changes from 0.1 to 0.5 mol/L,the current efficiency of La(OH)3 increases from 60%to 85%,the product roasted at 800℃for 1.5 h is La2O3,whose median particle size(D50)is 10-20μm,the mass fraction of chloride ion is less than 0.02 wt%.Additio nally,the prepared La2O3 particles are porous and non-spherical particle s,which are composed of small crystals with sheet structure.

关键词： Lanthanum chloride Membrane electrolysis Lanthanum hydroxide Lanthanum oxide Current efficiency Rare earths

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